15 results on '"Lucas Hunt"'
Search Results
2. CHILES: H imorphology and galaxy environment atz = 0.12 andz = 0.17
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Kelley M Hess, Nicholas M Luber, Ximena Fernández, Hansung B Gim, J H van Gorkom, Emmanuel Momjian, Julia Gross, Martin Meyer, Attila Popping, Luke J M Davies, Lucas Hunt, Kathryn Kreckel, Danielle Lucero, D J Pisano, Monica Sanchez-Barrantes, Min S Yun, Richard Dodson, Kevin Vinsen, Andreas Wicenec, Chen Wu, Matthew A Bershady, Aeree Chung, Julie D Davis, Jennifer Donovan Meyer, Patricia Henning, Natasha Maddox, Evan T Smith, J M van der Hulst, Marc A W Verheijen, and Eric M Wilcots
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- 2018
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3. Immortalized mammosphere-derived epithelial cells retain a bioactive secretome with antimicrobial, regenerative, and immunomodulatory properties
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Nikola Danev, Julia M. Poggi, Emilie A. Dewever, Arianna P. Bartlett, Leane Oliveira, Lucas Huntimer, Rebecca M. Harman, and Gerlinde R. Van de Walle
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Mammary epithelial cells ,Secretome ,Immortalization ,Immunomodulation ,Antimicrobial effects ,Fibroblast migration ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background The secretome of primary bovine mammosphere-derived epithelial cells (MDECs) has been shown to exert antimicrobial, regenerative, and immunomodulatory properties in vitro, which warrants its study as a potential biologic treatment with the potential to be translated to human medicine. Currently, the use of the MDEC secretome as a therapy is constrained by the limited life span of primary cell cultures and the decrease of secretome potency over cell passages. Methods To address these limitations, early-passage bovine MDECs were immortalized using hTERT, a human telomerase reverse transcriptase. The primary and immortal MDECs were compared morphologically, transcriptomically, and phenotypically. The functional properties and proteomic profiles of the secretome of both cell lines were evaluated and compared. All experiments were performed with both low and high passage cell cultures. Results We confirmed through in vitro experiments that the secretome of immortalized MDECs, unlike that of primary cells, maintained antimicrobial and pro-migratory properties over passages, while pro-angiogenic effects of the secretome from both primary and immortalized MDECs were lost when the cells reached high passage. The secretome from primary and immortalized MDECs, at low and high passages exerted immunomodulatory effects on neutrophils in vitro. Conclusions High passage immortalized MDECs retain a bioactive secretome with antimicrobial, regenerative, and immunomodulatory properties, suggesting they may serve as a consistent cell source for therapeutic use.
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- 2024
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4. PetroFit: A Python Package for Computing Petrosian Radii and Fitting Galaxy Light Profiles
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Robel Geda, Steven M. Crawford, Lucas Hunt, Matthew Bershady, Erik Tollerud, and Solohery Randriamampandry
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Space and Planetary Science ,Astrophysics of Galaxies (astro-ph.GA) ,FOS: Physical sciences ,Astronomy and Astrophysics ,Astrophysics::Cosmology and Extragalactic Astrophysics ,Astrophysics - Instrumentation and Methods for Astrophysics ,Astrophysics - Astrophysics of Galaxies ,Instrumentation and Methods for Astrophysics (astro-ph.IM) ,Astrophysics::Galaxy Astrophysics - Abstract
PetroFit is an open-source Python package, based on Astropy and Photutils, that can calculate Petrosian profiles and fit galaxy images. It offers end-to-end tools for making accurate photometric measurements, estimating morphological properties, and fitting 2D models to galaxy images. Petrosian metric radii can be used for model parameter estimation and aperture photometry to provide accurate total fluxes. Correction tools are provided for improving Petrosian radii estimates affected by galaxy morphology. PetroFit also provides tools for sampling Astropy-based models (including custom profiles and multi-component models) onto image grids and enables PSF convolution to account for the effects of seeing. These capabilities provide a robust means of modeling and fitting galaxy light profiles. We have made the PetroFit package publicly available on GitHub (PetroFit/petrofit) and PyPi (pip install petrofit)., Comment: Published AJ, 28 pages, 17 figures, see https://github.com/PetroFit/petrofit; typos corrected, footnote on PSFModel class, paper status update, DOI provided
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- 2022
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5. The mammosphere-derived epithelial cell secretome modulates neutrophil functions in the bovine model
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Rebecca M. Harman, Anja Sipka, Kelly A. Oxford, Leane Oliveira, Lucas Huntimer, Daryl V. Nydam, and Gerlinde R. Van de Walle
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bovine ,mammosphere-derived epithelial cells ,neutrophils ,secretome ,host-directed immunotherapy ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundInnovative therapies against bacterial infections are needed. One approach is to focus on host-directed immunotherapy (HDT), with treatments that exploit natural processes of the host immune system. The goals of this type of therapy are to stimulate protective immunity while minimizing inflammation-induced tissue damage. We use non-traditional large animal models to explore the potential of the mammosphere-derived epithelial cell (MDEC) secretome, consisting of all bioactive factors released by the cells, to modulate host immune functions. MDEC cultures are enriched for mammary stem and progenitor cells and can be generated from virtually any mammal. We previously demonstrated that the bovine MDEC secretome, collected and delivered as conditioned medium (CM), inhibits the growth of bacteria in vitro and stimulates functions related to tissue repair in cultured endothelial and epithelial cells.MethodsThe immunomodulatory effects of the bovine MDEC secretome on bovine neutrophils, an innate immune cell type critical for resolving bacterial infections, were determined in vitro using functional assays. The effects of MDEC CM on neutrophil molecular pathways were explored by evaluating the production of specific cytokines by neutrophils and examining global gene expression patterns in MDEC CM-treated neutrophils. Enzyme linked immunosorbent assays were used to determine the concentrations of select proteins in MDEC CM and siRNAs were used to reduce the expression of specific MDEC-secreted proteins, allowing for the identification of bioactive factors modulating neutrophil functions.ResultsNeutrophils exposed to MDEC secretome exhibited increased chemotaxis and phagocytosis and decreased intracellular reactive oxygen species and extracellular trap formation, when compared to neutrophils exposed to control medium. C-X-C motif chemokine 6, superoxide dismutase, peroxiredoxin-2, and catalase, each present in the bovine MDEC secretome, were found to modulate neutrophil functions.ConclusionThe MDEC secretome administered to treat bacterial infections may increase neutrophil recruitment to the site of infection, stimulate pathogen phagocytosis by neutrophils, and reduce neutrophil-produced ROS accumulation. As a result, pathogen clearance might be improved and local inflammation and tissue damage reduced.
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- 2024
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6. The Evolution of the Luminosity Function for Luminous Compact Blue Galaxies to z=1
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Daniel J. Pisano, Matthew A. Bershady, Lucas Hunt, Gregory D. Wirth, and Steven M. Crawford
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010504 meteorology & atmospheric sciences ,media_common.quotation_subject ,Population ,FOS: Physical sciences ,Astrophysics ,Astrophysics::Cosmology and Extragalactic Astrophysics ,01 natural sciences ,Luminosity ,0103 physical sciences ,Galaxy formation and evolution ,Astrophysics::Solar and Stellar Astrophysics ,education ,010303 astronomy & astrophysics ,Astrophysics::Galaxy Astrophysics ,0105 earth and related environmental sciences ,media_common ,Luminosity function (astronomy) ,Physics ,education.field_of_study ,Star formation ,Astronomy and Astrophysics ,Astrophysics - Astrophysics of Galaxies ,Galaxy ,Redshift ,Universe ,Space and Planetary Science ,Astrophysics of Galaxies (astro-ph.GA) - Abstract
Luminous Compact Blue Galaxies (LCBGs) are compact, star-forming galaxies that are rarely observed in the local universe but abundant at z=1. This increase in LCBG number density over cosmic lookback time roughly follows the increase in the star formation rate density of the universe over the same period. We use publicly available data in the COSMOS field to study the evolution of the largest homogeneous sample of LCBGs to date by deriving their luminosity function in four redshift bins over the range $0.1\leq~z\leq1$. We find that over this redshift range, the characteristic luminosity (M$^{*}$) increases by $\sim$0.2 mag, and the number density increases by a factor of four. While LCBGs make up only about $18\%$ of galaxies more luminous than M$_{B}=-$18.5 at $z\sim0.2$, they constitute roughly $54\%$ at z$\sim$0.9. The strong evolution in number density indicates that LCBGs are an important population of galaxies to study in order to better understand the decrease in the star formation rate density of the universe since $z\sim1$., 19 pages, 12 Figures, Accepted in ApJ
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- 2021
7. The H<scp>i</scp> Neighborhoods Around STARBIRDS
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John M. Cannon, Megan Johnson, Tobias Westmeier, Jeremy Bailin, Charlotte P. Martinkus, Kristen B. W. McQuinn, P. Kamphuis, Evan D. Skillman, O. Ivy Wong, Lucas Hunt, and H. Alyson Ford
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Physics ,Spiral galaxy ,Star formation ,Green Bank Telescope ,Astronomy and Astrophysics ,Astrophysics::Cosmology and Extragalactic Astrophysics ,Astrophysics ,Galaxy ,law.invention ,Telescope ,Gravitation ,Space and Planetary Science ,law ,Astrophysics::Solar and Stellar Astrophysics ,Astrophysics::Earth and Planetary Astrophysics ,Surface brightness ,Astrophysics::Galaxy Astrophysics ,Dwarf galaxy - Abstract
Starbursts are finite periods of intense star formation (SF) that can dramatically impact the evolutionary state of a galaxy. Recent results suggest that starbursts in dwarf galaxies last longer and are distributed over more of the galaxy than previously thought, with star formation efficiencies (SFEs) comparable to spiral galaxies, much higher than those typical of non-bursting dwarfs. This difference might be explainable if the starburst mode is externally triggered by gravitational interactions with other nearby systems. We present new, sensitive neutral hydrogen observations of 18 starburst dwarf galaxies, which are part of the STARburst IRregular Dwarf Survey (STARBIRDS) and each were mapped with the Green Bank Telescope (GBT) and/or Parkes Telescope in order to study the low surface brightness gas distributions, a common tracer for tidal interactions.
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- 2018
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8. The Evolution of Luminous Compact Blue Galaxies in COSMOS between z~0.0-1.0
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Lucas Hunt
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Physics ,Cosmos (category theory) ,Astronomy ,Galaxy - Published
- 2019
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9. CHANG-ES XX. High Resolution Radio Continuum Images of Edge-on Galaxies and their AGNs -- Data Release 3
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Timothy T. Braun, Philip Schmidt, Marita Krause, Arpad Miskolczi, Ralf-Jürgen Dettmar, Alison Merritt, Theresa Wiegert, Marek Wezgowiec, Mark L. A. Richardson, Jiang-Tao Li, Lucas Hunt, Yelena Stein, Megan Johnson, D. J. Saikia, Ancor Damas-Segovia, Alex Woodfinden, Jisung Im, Jayanne English, Q. Daniel Wang, Judith A. Irwin, Observatoire astronomique de Strasbourg (ObAS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), and Natural Sciences and Engineering Research Council of Canada
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010504 meteorology & atmospheric sciences ,Astrophysics::High Energy Astrophysical Phenomena ,High resolution ,FOS: Physical sciences ,ComputingMilieux_LEGALASPECTSOFCOMPUTING ,Astrophysics ,galaxies [Radio continuum] ,Astrophysics::Cosmology and Extragalactic Astrophysics ,Edge (geometry) ,Surveys ,01 natural sciences ,Radio continuum: galaxies ,0103 physical sciences ,ComputingMilieux_COMPUTERSANDEDUCATION ,Natural science ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,010303 astronomy & astrophysics ,ComputingMilieux_MISCELLANEOUS ,Astrophysics::Galaxy Astrophysics ,0105 earth and related environmental sciences ,Physics ,ComputingMilieux_THECOMPUTINGPROFESSION ,Continuum (topology) ,Astronomy and Astrophysics ,Astrophysics - Astrophysics of Galaxies ,Galaxy ,Space and Planetary Science ,[SDU]Sciences of the Universe [physics] ,Astrophysics of Galaxies (astro-ph.GA) ,Data release - Abstract
The CHANG-ES galaxy sample consists of 35 nearby edge-on galaxies that have been observed using the VLA at 1.6 and 6.0 GHz. Here we present the third data release of our sample, namely, the B configuration 1.6 GHz sample. In addition, we make available the band-to-band spectral index maps between 1.6 and 6.0 GHz, the latter taken in the matching resolution C configuration. The images can be downloaded from https://www.queensu.ca/changes. These are our highest-resolution images (≈3″), and we examine the possible presence of low-luminosity active galactic nuclei (AGNs) in the sample, as well as some in-disk structure. New features can be seen in the spectral index maps that are masked in the total intensity emission, including hidden spiral arms in NGC 3448 and two previously unknown radio lobes on either side of the nucleus of NGC 3628. Our AGN detection rate, using only radio criteria, is 55%, which we take as a lower limit because some weaker embedded AGNs are likely present that could be revealed at higher resolution. Archival XMM-Newton data were used to search for further fingerprints of the AGNs in the studied sample. In galaxy disks, discrete regions of flat spectral index are seen, likely due to a thermal emission fraction that is higher than the global average.© 2019. The American Astronomical Society. All rights reserved., The first author would like to thank the Natural Sciences and Engineering Research Council of Canada for a Discovery Grant.
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- 2019
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10. Fundamental Reference AGN Monitoring Experiment (FRAMEx). I. Jumping Out of the Plane with the VLBA
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Megan Johnson, Norbert Zacharias, Nathan J. Secrest, Lucas Hunt, Henrique R. Schmitt, Travis C. Fischer, Bryan N. Dorland, Michael Koss, Luis C. Fernandez, and Phillip J. Cigan
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Active galactic nucleus ,010504 meteorology & atmospheric sciences ,Astrophysics::High Energy Astrophysical Phenomena ,FOS: Physical sciences ,Astrophysics::Cosmology and Extragalactic Astrophysics ,Astrophysics ,01 natural sciences ,Luminosity ,law.invention ,Jansky ,Telescope ,law ,0103 physical sciences ,010303 astronomy & astrophysics ,Astrophysics::Galaxy Astrophysics ,Very Long Baseline Array ,0105 earth and related environmental sciences ,Line (formation) ,High Energy Astrophysical Phenomena (astro-ph.HE) ,Physics ,Astronomy and Astrophysics ,Astrophysics - Astrophysics of Galaxies ,Black hole ,Space and Planetary Science ,Astrophysics of Galaxies (astro-ph.GA) ,Astrophysics - High Energy Astrophysical Phenomena ,Fundamental plane (elliptical galaxies) - Abstract
We present the first results from the Fundamental Reference AGN Monitoring Experiment (FRAMEx), an observational campaign dedicated to understanding the physical processes that affect the apparent positions and morphologies of AGNs. In this work, we obtained simultaneous Swift X-ray Telescope (XRT) and Very Long Baseline Array (VLBA) radio observations for a snapshot campaign of 25 local AGNs that form a volume-complete sample with hard X-ray (14-195 keV) luminosities above $10^{42}$erg s$^{-1}$, out to a distance of 40 Mpc. Despite achieving an observation depth of $\sim20$ $\mu$Jy, we find that 16 of 25 AGNs in our sample are not detected with the VLBA on milli-arcsecond (sub-parsec) scales, and the corresponding core radio luminosity upper limits are systematically below predictions from the Fundamental Plane of black hole activity. Using archival Jansky Very Large Array (VLA) radio measurements, our sample jumps back onto the Fundamental Plane, suggesting that extended radio emission is responsible for the apparent correlation between radio emission, X-ray emission, and black hole mass. We suggest that this discrepancy is likely due to extra-nuclear radio emission produced via interactions between the AGN and host environment. We compare VLBA observations of AGNs to VLA observations of nearby Galactic black holes (GBHs) and we find a mass-independent correlation between radio and X-ray luminosities of black holes of $L_\mathrm{6cm}$/$L_\mathrm{2-10 keV}$ $\sim$ 10$^{-6}$, in line with predictions for coronal emission, but allowing for the possibility of truly radio silent AGNs., Comment: 19 pages + 9 page Appendix, 18 figures, accepted for publication in ApJ
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- 2021
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11. Bovine milk-derived cells express transcriptome markers of pluripotency and secrete bioactive factors with regenerative and antimicrobial activity
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Nikola Danev, Rebecca M. Harman, Leane Oliveira, Lucas Huntimer, and Gerlinde R. Van de Walle
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Medicine ,Science - Abstract
Abstract The bovine mammary stem/progenitor cell secretome stimulates regeneration in vitro and contains proteins associated with antimicrobial defense. This has led to the exploration of the secretome as a biologic treatment for mastitis, a costly inflammation of the udder commonly caused by bacteria. This study reports on a population of bovine mammary stem/progenitor cells isolated non-invasively from milk (MiDCs). MiDCs were characterized by immunophenotyping, mammosphere formation assays, and single cell RNA sequencing. They displayed epithelial morphology, exhibited markers of mammary stem/progenitor cells, and formed mammospheres, like mammary gland tissue-isolated stem/progenitor cells. Single cell RNA sequencing revealed two sub-populations of MiDCs: epithelial cells and macrophages. Functionally, the MiDC secretome increased fibroblast migration, promoted angiogenesis of endothelial cells, and inhibited the growth of mastitis-associated bacteria, including antibiotic-resistant strains, in vitro. These qualities of MiDCs render them a source of stem cells and stem cell products that may be used to treat diseases affecting the dairy industry, including mastitis.
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- 2023
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12. Highest Redshift Image of Neutral Hydrogen in Emission: A CHILES Detection of a Starbursting Galaxy at z = 0.376
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Jay Strader, R. Salinas, Kathryn Kreckel, Julia Gross, Christopher A. Hales, Richard Dodson, Ximena Fernández, J. H. van Gorkom, Andreas Wicenec, Daniel J. Pisano, Natasha Maddox, Jennifer Donovan Meyer, Marc Verheijen, Evangelia Tremou, R. Chávez, Attila Popping, Martin Meyer, Lucas Hunt, D. M. Lucero, Min S. Yun, K. Golap, Aeree Chung, Emmanuel Momjian, Patricia A. Henning, Matthew A. Bershady, Yara L. Jaffé, Monica Sanchez-Barrantes, David Schiminovich, John E. Hibbard, Kelley M. Hess, Nick Scoville, Eric M. Wilcots, Tom Oosterloo, Laura Chomiuk, Hansung B. Gim, and Astronomy
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Stellar mass ,Large Millimeter Telescope ,FOS: Physical sciences ,galaxies: starburst ,Astrophysics ,H-I ,MU-M ,SCALING RELATIONS ,01 natural sciences ,TELESCOPE ADVANCED CAMERA ,Radio telescope ,COSMOS SURVEY ,0103 physical sciences ,MOLECULAR GAS CONTENT ,Galaxy formation and evolution ,STAR-FORMING GALAXIES ,010306 general physics ,010303 astronomy & astrophysics ,Physics ,Luminous infrared galaxy ,radio lines: galaxies ,LEGACY SURVEY ,Astronomy and Astrophysics ,Astrophysics - Astrophysics of Galaxies ,Galaxy ,Redshift ,Accretion (astrophysics) ,ATOMIC GAS ,Space and Planetary Science ,Astrophysics of Galaxies (astro-ph.GA) ,galaxies: evolution ,STELLAR MASS - Abstract
Our current understanding of galaxy evolution still has many uncertainties associated with the details of accretion, processing, and removal of gas across cosmic time. The next generation of radio telescopes will image the neutral hydrogen (HI) in galaxies over large volumes at high redshifts, which will provide key insights into these processes. We are conducting the COSMOS HI Large Extragalactic Survey (CHILES) with the Karl G. Jansky Very Large Array, which is the first survey to simultaneously observe HI from z=0 to z~0.5. Here, we report the highest redshift HI 21-cm detection in emission to date of the luminous infrared galaxy (LIRG) COSMOS J100054.83+023126.2 at z=0.376 with the first 178 hours of CHILES data. The total HI mass is $(2.9\pm1.0)\times10^{10}~M_\odot$, and the spatial distribution is asymmetric and extends beyond the galaxy. While optically the galaxy looks undisturbed, the HI distribution suggests an interaction with candidate a candidate companion. In addition, we present follow-up Large Millimeter Telescope CO observations that show it is rich in molecular hydrogen, with a range of possible masses of $(1.8-9.9)\times10^{10}~M_\odot$. This is the first study of the HI and CO in emission for a single galaxy beyond z~0.2., 7 pages, 5 figures, accepted for publication in ApJL
- Published
- 2016
13. Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites
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Benjamin J. Evert, Shuxiong Chen, Robyn McConville, Ryan W. J. Steel, Julie Healer, Justin A. Boddey, Lucas Huntimer, and Bernd H. A. Rehm
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Immunologic diseases. Allergy ,RC581-607 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract The current Malaria RTS,S vaccine is based on virus-like particles (VLPs) comprising the NANP repetitive epitopes from the cicumsporozoite protein (CSP) of Plasmodium falciparum. This vaccine has limited efficacy, only preventing severe disease in about 30% of vaccinated individuals. A more efficacious vaccine is urgently needed to combat malaria. Here we developed a particulate malaria vaccine based on the same CSP epitopes but using biopolymer particles (BPs) as an antigen carrier system. Specific B- and T-cell epitope-coated BPs were assembled in vivo inside an engineered endotoxin-free mutant of Escherichia coli. A high-yield production process leading to ~27% BP vaccine weight over biomass was established. The epitope-coated BPs were purified and their composition, i.e., the polymer core and epitope identity, was confirmed. Epitope-coated BPs were used alongside soluble peptide epitopes and empty BPs to vaccinate sheep. Epitope-coated BPs showed enhanced immunogenicity by inducing anti-NANP antibody titre of EC50 > 150,000 that were at least 20 times higher than induced by the soluble peptides. We concluded that the additional T-cell epitope was not required as it did not enhance immunogenicity when compared with the B-cell epitope-coated BPs. Antibodies specifically bound to the surface of Plasmodium falciparum sporozoites and efficiently inhibited sporozoite motility and traversal of human hepatocytes. This study demonstrated the utility of biologically self-assembled epitope-coated BPs as an epitope carrier for inclusion in next-generation malaria vaccines.
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- 2021
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14. CHANG-ES. XX. High-resolution Radio Continuum Images of Edge-on Galaxies and Their AGNs: Data Release 3.
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Judith Irwin, Theresa Wiegert, Alison Merritt, Marek Weżgowiec, Lucas Hunt, Alex Woodfinden, Yelena Stein, Ancor Damas-Segovia, Jiangtao Li, Q. Daniel Wang, Megan Johnson, Marita Krause, Ralf-Jürgen Dettmar, Jisung Im, Philip Schmidt, Arpad Miskolczi, Timothy T. Braun, D. J. Saikia, Jayanne English, and Mark Richardson
- Published
- 2019
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15. HIGHEST REDSHIFT IMAGE OF NEUTRAL HYDROGEN IN EMISSION: A CHILES DETECTION OF A STARBURSTING GALAXY AT z = 0.376.
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Ximena Fernández, Hansung B. Gim, J. H. van Gorkom, Min S. Yun, Emmanuel Momjian, Attila Popping, Laura Chomiuk, Kelley M. Hess, Lucas Hunt, Kathryn Kreckel, Danielle Lucero, Natasha Maddox, Tom Oosterloo, D. J. Pisano, M. A. W. Verheijen, Christopher A. Hales, Aeree Chung, Richard Dodson, Kumar Golap, and Julia Gross
- Published
- 2016
- Full Text
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