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The Sumerian culture flourished within the Tigris and Euphrates rivers floodplains and along their deltaic systems, which ca. 6000 yr were located ~250–260 km inland from the present Persian Gulf. Here, large floodplains and marshes were crossed by an intricate network of channels where several human settlements developed. In this paper, we describe in detail the paleoenvironmental context where the site of Abu Tbeirah (third millennium BC) developed, near the Sumerian capital of Ur. Our interdisciplinary approach, based on remote sensing and the geomorphological study of the area, as well as on sedimentological, paleontological, and paleobotanical analyses of trenches and boreholes deposits, reveals that the site developed along a sinuous channel in a floodplain and marshy environment, where several crevasse splays occurred. This channel was cut off following a flood event. The abandoned portion of the channel was exploited by residents and used as a small river harbor. Our research contributes to better define how the landscape of the site changed over the course of its history and how humans exploited water resources of the area during occupation of the site, a process that was pivotal for the development of the Sumerian culture.

Angiogenesis is target for antineoplastic and chemopreventive therapies. The natural phytoalexin resveratrol is found in grapes and red wine as cis and trans stereoisomers. trans-Resveratrol shows antiangiogenic activity, but its mechanism of action is not fully elucidated. Recently, trans-resveratrol has been shown to interact with the β3 integrin subunit, raising the possibility that inhibition of endothelial αvβ3 integrin function may concur to its angiosuppressive activity. To get novel insights about the antiangiogenic activity of resveratrol, we compared cis- and trans-resveratrol stereoisomers for their effect on the angiogenesis process and endothelial αvβ3 integrin function. trans-Resveratrol inhibits endothelial cell proliferation and the repair of mechanically wounded endothelial cell monolayers. Also, it prevents endothelial cell sprouting in fibrin gel, collagen gel invasion, and morphogenesis on Matrigel. In vivo, trans-resveratrol inhibits vascularization of the chick embryo area vasculosa and murine melanoma B16 tumor growth and neovascularization. In all the assays, cis-resveratrol exerts a limited, if any, effect. In keeping with these observations, trans-resveratrol, but not cis-resveratrol, inhibits αvβ3 integrin-dependent endothelial cell adhesion and the recruitment of enhanced green fluorescent protein-tagged β3 integrin in focal adhesion contacts. In conclusion, stereoisomery affects the antiangiogenic activity of resveratrol, the trans isomer being significantly more potent than the cis isoform. The different antiangiogenic potential of resveratrol stereoisomers is related, at least in part, to their different capacity to affect αvβ3 integrin function. This may have profound implications for the design of synthetic antiangiogenic/angiopreventive phytoalexin derivatives. [Mol Cancer Ther 2008;7(12):3761–70]

Supplementary Fig. S1 from αvβ3 Integrin-dependent antiangiogenic activity of resveratrol stereoisomers

During the Assyrian rule over Northern Mesopotamia (present-day northern Iraq), complex systems of canals were built to increase the surface of cultivable land and improve the movement of people and goods across the irrigated waterscape of the hinterland of Nineveh and other major urban centres, with the aim of granting socio-economic prosperity to the empire. Nevertheless, supra-regional political instability eventually led to the rapid downfall of the Assyrian state during the late seventh century BCE, causing the swift abandonment of the canal systems. In this study, we examine the post-abandonment formation processes of the natural and anthropogenic infillings of three portions of King Sennacherib’s Khinis canal system (705–681 BCE, northwestern Kurdistan Region of Iraq) through means of archaeological, sedimentological and micromorphological analyses. We identify water lain sediments, desiccation features, colluvial gravel intake and pastoral occupation layers, anchoring the deposit to radiometric dating and contextualising it against the known regional climatic history. The interpretation of pedostratigraphic evidence highlights subsequent phases of use, abandonment, and repurposing of the canals, reflecting shifts in land-use from agriculture to pastoralism and dynamic adaptation and resilience of the local ancient communities in response to Late-Holocene climate changes and geopolitical events.

In a perspective projected to reduce the atmospheric concentration of greenhouse gases, in which carbon dioxide is the master, the use of microalgae is an effective and decisive response. The review describes the bio circularity of the process of abatement of carbon dioxide through biofixation in algal biomass, highlighting the potential of its reuse in the production of high value-added products.

During the last century, Grotta Romanelli (Southern Italy) has been a reference site for the European Late Pleistocene stratigraphy, due to its geomorphological setting and archaeological and palaeontological content. The beginning of the sedimentation inside the cave was attributed to MISs 5e and the oldest unearthed evidence of human occupation, including remains of hearths, was therefore referred to the Middle Palaeolithic. Recent surveys and excavations produced new U/Th dates, palaeoenvironmental interpretation and a litho-, morpho- and chrono-stratigraphical reassessment, placing the oldest human frequentation of the cave between MIS 9 and MIS 7, therefore embracing Glacial and Interglacial cycles. These new data provide evidence that the sea reached the cave during the Middle Pleistocene and human occupation occurred long before MISs 5e and persisted beyond the Pleistocene- Holocene boundary.

Since the Pleistocene, the Mediterranean is a hot spot for climate change and human migrations, thus offering the opportunity to investigate how human populations have responded to environmental changes and sea level variations. This is the main topic of the SPHeritage Project (MUR grant: FIRS2019_00040, P.I.: M. Pappalardo) that proposes an interdisciplinary approach to investigate the human-environment interaction (in particular sea level variations) over the last 400,000 years using a combination of micro-invasive methods. The Project is re-investigating the well-known archaeological area of ​​the Balzi Rossi (Ventimiglia, at the border between Italy and France), which represents a unique assemblage of archaeological sites dating to the Palaeolithic, distributed in a geomorphological setting rich of markers of past sea level changes. As most of the local archaeological sequences have been extensively investigated at the beginning of the last century and large part of the deposits removed, we will combine the analyses of materials preserved in museums (including strips of sediments) and the remnants still preserved inside many rockshelters and caves of the archaeological complex. Moreover, our geomorphological survey identified new sedimentary sequences preserving information on relative sea level changes. This approach will permit to obtain innovative data submitting small samples to state-of-the-art methods for dating and palaeoenvironmental reconstruction, thus offering the opportunity to better constrain the time and steps of climate change, sea level oscillations, and human settlements. Moreover, data will converge into geoheritage analyses aimed at finding the best practices for promoting and protecting the site. Here, we present an overview of the project and preliminary results from some of the major archaeological sites.

Located along the Tigris River in the Kurdistan Region of Iraq (KRI), the Mosul Dam reservoir is the second biggest dam of the Near East, and represents an important water storage for local human activities. The Dam was built between 1981 and 1988 north of the village of Eski Mosul; along this part of the Tigris River several archaeological sites were inundated. Analysis of historical images derived from Declassified Corona satellite acquired between December 1967 and August 1968 reveals seasonal changes of the Tigris riverbed, shifting across the hydrological year from meandering to anastomosing. The geomorphological mapping was carried out on the December 1967 and. in August 1968, Corona images were taken, in order to estimate the modification of several fluvial geomorphological elements such as floodplain and point, middle and longitudinal bars. Here, such evidence is compared with Landsat data collected between the 1990ies and today, in order to detect the first phases of filling of the basin and the control of inherited Tigris channel belt over the reservoir. Moreover, we also noticed an influence of the ancient Tigris course on is recent insertion into the lake. Our work permitted to reconstruct the ancient fluvial landscape below the Mosul Dam Lake, and its evolution in response to seasonal variation of the discharge.

Marine fireworms (Annelida, Amphinomidae) are notorious for their stinging dorsal bristles (notochaetae). In the large-size species Hermodice carunculata, characterized by invasive potential and great predatory ability, notochaetae deter consumers and damage prey. The origin of fireworm defensive and offensive capacities, the mechanism of action of the stinging chaetae, and whether other chemical defenses occur are still uncertain. In the present study, we tested if the urticating system of fireworms is the result of a synergy between mechanical injury, due to notochaetae penetration, and the release of toxins vehiculated. To determine whether and where chemical or structural deterrents were present, different body parts of H. carunculata involved in inter-specific interactions were assayed (i.e. dorsal body wall, gills, dorsal and ventral parapodia). Feeding bioassays were performed to assess palatability against a fish (generalist consumer) and deterrent action on contact with two sea anemones (fireworm prey). In all the assays, only the offers bearing the dorsal chaetae (notochaetae) strongly deterred consumers and induced paralysis in prey. The notochaetae treated with organic solvents lost their deterrence against fish. Besides, the notopodia were palatable to fish and anemones after the removal of the notochaetae. These findings support a powerful synergy between chemical deterrence and physical structure. To date, the only acute inflammation inducer isolated from an amphinomid is “complanine”, a trimethylammonium compound. High-resolution LC-MS/MS and preliminary NMR analyses suggested the presence of complanine-related compounds in the notochaetae. The present results suggest that the release of compounds and their strong deterrent effect should be mediated by mechanical injury. The sharp, fragile and hollow structure of the notochaetae combined with inflammatory substances could support the success of fireworms in marine benthic environments. Further research is required to ascertain the nature and localization of these chemicals in H. carunculata, broadening knowledge on the chemical ecology of amphinomids.

In a very recent book called Sensory Biology of Plants, published by renowned publisher Springer Nature, the authors stated that the scientific literature gathered so far regarding knowledge around the field of Plant Acoustics allows us to divert the focus from the question “whether plants perceive sound” toward the questions “how and why they are doing it” Some phenomena are well known: roots perceive the sound of flowing water and display a sound-mediated growth toward the water source, while the buzz pollination process allows plants to minimize the pollen lost and maximize which is collected by true pollinators. But plants are far more perceptive and responsive to their environment than we generally consider them to be, and they are communicating far more information than we realize if we only took all their signals (VOCs, sound, exudates, etc.) into a greater picture. Could Volatile Organic Compounds (VOCs) be involved in mediating more responses than we imagine? VOC synthesis and release is known to be elicited also by electrical signals caused by mechanical stimuli, touching and wounding being among these, serving as info-chemicals in the communication between plants (“eavesdropping”), and within the organs of the same plant, in order for it to get synchronized with its surroundings. This paper is an overview of the discoveries around plant perception with a focus on the link between mechanical stimuli, as sound vibrations are, and changes in plant physiology leading to VOC emission.

Life evolved in an acoustic world. Sound is perceived in different ways by the species that inhabit the Planet. Among organisms, also some algal species seem to respond to sound stimuli with increased cell growth and productivity. The purpose of this Short Communication is to provide an overview of the current literature about various organisms and sound, with particular attention to algal organisms, which, when subjected to sound applications, can change their metabolism accordingly.

During the late Quaternary, Iraqi Kurdistan was the scenario of several fundamental human-relatedevents including the dispersion of Homo in Asia and Europe, the origin of agriculture, the beginningof urbanization, and the formation of the first state entities. We present the initial results of ageoarchaeological investigation in this area, which aims to reconstruct a detailed framework of therelationship between climatic changes, landscape responses, human adaptation, and settlementdistribution during the Late Quaternary. Paleoenvironmental and paleoclimatic data were collectedfrom two key areas: the territory of the Navkur and Faideh plains, in northern Kurdistan, and a portionof the Erbil plain, in southern Kurdistan. In the two regions, the Land of Niniveh and MAIPEarchaeological missions are operating. Remote sensing, GIS analyses, and geomorphological surveyare the tools used for the geomorphological reconstruction of ancient hydrology (fluvial pattern) andthe evolution of distinct landforms. Geochemical and geochronological analyses on speleothems fromthe Zagros piedmont caves of same region provide information on Holocene climatic variability inthe area. Whereas environmental settings and human land use are investigated on the basis ofsedimentological, palynological, micropaleontological, and geochemical analyses of a fluvio-lacustrine sequences preliminary dated between 40 and 9 ka BP. The lacustrine sequence is composedby clayey and silt-sandy sediments alternating calcareous and organic matter-rich layers.Environmental and geomorphological data have been compared with archaeological information(mostly the chronological distribution of the archaeological sites) to interpret exploitation of naturalresources, the settlement dynamics and shift in land use.

Fruits and vegetables are a good source of potentially biologically active compounds. Their regular consumption in the human diet can help reduce the risk of developing chronic diseases such as cardiovascular diseases and cancer. Plants produce additional chemical substances when subject to abiotic stress or infected by microorganisms. The phytochemical profile of spinach leaves (Spinacia oleracea L.), which is a vegetable with widely recognized health-promoting activity, has been affected by applying root hypoxic and re-oxygenation stress during plant growth. Leaf juice at different sampling times has been subject to liquid chromatography mass spectrometry (LC-MSn) analysis and tested on the human colorectal adenocarcinoma cell line HT29 by using the Comet assay. The cells were previously treated with H2O2 to simulate the presence of an oxidative stress (as in colon cancer condition) and the leaf juice application resulted in a significant antioxidant and protective in vitro effect. The duration of the hypoxic/re-oxygenation stress imposed on the plant reflects the antioxidant leaf juice content. After hypoxic stress (24 h) and reoxygenation (2 h), we show a decrease (50%) of the relative abundance of the principal identified antioxidant molecules but a higher antioxidant activity of the spinach juice on HT29 cells (20%). Data shows a complex relation between plant growing conditions and the modulation of secondary metabolites content in leaf juice that results in different chemo-protective activities in colon cancer cells.

The rising consumer demand for “natural” foodstuffs has encouraged a growing part of both the academic and industrial scientific communities to develop novel biocatalysts for producing flavoring molecules. In this context, nonconventional yeasts (NCYs) have attracted increasing interest due to their biochemical characteristics and potential applications, being able to produce aroma compounds from a variety of carbon sources, including sugars, alkanes, plant oils, starch hydrolysates, ethanol, and glycerol. Apart from classical fermentation processes (de novo synthesis), bioconversion of appropriate precursor compounds are also being developed to produce food aromas. An overview on the potential of NCYs’ whole cell for producing food flavors by biotransformation is illustrated in this chapter by a discussion of the production of different class of compounds, namely alcohols, aldehydes, ketones, lactones, terpenes, and terpenoids, alkenes, phenols, and sulfur compounds.

The antibacterial activity of Pimpinella anisum L., Cinnamomum zeylanicum, Syzygium aromaticum, and Cuminum cyminum L. essential oils (EOs) against some common pathogenic microorganisms (Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212, Streptococcus pyogenes ATCC 1915, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 27853, Aeromonas hydrophila ATCC 7966, Proteus mirabilis ATCC 10005, Klebsiella pneumoniae ATCC 13883, and Candida albicans ATCC 10231) and their biofilms was studied. The EOs inhibitory effects were evaluated by both Agar Well Diffusion assay and Minimum Inhibitory Concentration (MIC) determination. The most active EOs, cinnamon and cloves, were also tested on 18, 24, 48, 72 hours mature biofilms. Cinnamon and cloves exhibited the best results showing a significant activity against all the tested bacteria. Concerning biofilm, results suggest that Cinnamomum zeylanicum oil may be a useful approach to impair the biofilm produced by the tested Gram-negative bacteria.

In this study a photobioreactor prototype is presented for the culture growth of microalgae model organism Neochloris oleoabundans by using chicken manure waste as feedstock along with the optimum combination of led light wavelengths and light intensity. Particularly interesting results are observed on the strains fed by chicken manure medium under the proper combination of red and blue LED light illumination, the microalgal growth resulted comparable with the strains fed by the costly commercial microalgal growth medium (BG 11 medium). Cell concentration, optical density, growth rate, cell size, total lipid and photosynthetic pigment content have been monitored during a time-course experiment. The data suggest that there are difficulties due to white light diffusion into the dark chicken medium, which leads to a generally lower intensity scattered along all wavelengths; blue or combined red and blue lights resulted in a higher irradiation density, affecting microalgae cell growth.

Mucus secretions play a number of functions related to polychaete physiology and ecology. Under stress conditions, the polychaete Halla parthenopeia (Oenonidae) produces a purple mucus after mechanical stimulation, whose function is still unknown. Here, we assessed the toxicity of this purple mucus by means of both acute toxicity bioassays on the polychaete Dinophilus gyrociliatus and commercial ecotoxicological kits (Microtox®, Rotoxkit® and Artoxkit®). Palatability was also tested with the fish Oryzias melastigma . After emitting purple mucus, H. parthenopeia quickly moves away and starts releasing transparent mucus. Acute toxicity bioassays showed that the mucus was harmless (transparent), or lethal even when diluted about 1000 times (purple). Purple mucus was toxic at different concentrations, the LC50 ranging from 0.7-0.3 g l –1 for D. gyrociliatus to 76 g l –1 for Artemia franciscana (Artoxkit®). Freeze-dried brine shrimp coated with transparent or purple muci were both consumed by O. melastigma . We hypothesized that the purple mucus is involved in the chemical defence of H. parthenopeia against competitors and parasites, and that its colour and toxicity are due to hallachrome, a 1,2-anthraquinone found in the skin of H. parthenopeia .

BACKGROUND In the human diet, the consumption of fresh fruits and vegetables is important in maintaining good health and in preventing chronic diseases. It is known that plant-derived food is a powerful source of chemopreventive molecules, i.e. antioxidants, and spinach (Spinacia oleracea L., Chenopodiaceae) possesses a wide range of metabolites with such biological activity. Plant stress response could lead to the production of metabolites with high value for human health and this could be a tool to enhance the production of molecules with antioxidant activity in plants. RESULTS Data reported in this paper confirm the antioxidant properties of spinach plants, and show a strong antiproliferative activity of leaf extract on HT-29 human cell line. Besides, the hypoxic stress seems to affect the pool of antioxidant molecules present in spinach leaves, as verified by means of HPLC-MS/MS analysis and the aluminium chloride and ABTS assays. CONCLUSION Our findings represent a basis for improving the biological and pharmacological properties of spinach plants, including the use of different growth conditions to modulate the phytocomplex profile of spinach. © 2014 Society of Chemical Industry

Triterpenoids are natural compounds synthesized by plants through cyclization of squalene, known for their weak anti-inflammatory activity. 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid (CDDO), and its C28 modified derivative, methyl-ester (CDDO-Me, also known as bardoxolone methyl), are two synthetic derivatives of oleanolic acid, synthesized more than 20 years ago, in an attempt to enhance the anti-inflammatory behavior of the natural compound. These molecules have been extensively investigated for their strong ability to exert antiproliferative, antiangiogenic, and antimetastatic activities, and to induce apoptosis and differentiation in cancer cells. Here, we discuss the chemical properties of natural triterpenoids, the pathways of synthesis and the biological effects of CDDO and its derivative CDDO-Me. At nanomolar doses, CDDO and CDDO-Me have been shown to protect cells and tissues from oxidative stress by increasing the transcriptional activity of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2). At doses higher than 100 nM, CDDO and CDDO-Me are able to modulate the differentiation of a variety of cell types, both tumor cell lines or primary culture cell, while at micromolar doses these compounds exert an anticancer effect in multiple manners; by inducing extrinsic or intrinsic apoptotic pathways, or autophagic cell death, by inhibiting telomerase activity, by disrupting mitochondrial functions through Lon protease inhibition, and by blocking the deubiquitylating enzyme USP7. CDDO-Me demonstrated its efficacy as anticancer drugs in different mouse models, and versus several types of cancer. Several clinical trials have been started in humans for evaluating CDDO-Me efficacy as anticancer and anti-inflammatory drug; despite promising results, significant increase in heart failure events represented an obstacle for the clinical use of CDDO-Me.

As part of a program aiming at the selection of yeast strains which might be of interest as sources of natural flavours and fragrances, the bioreduction of (4R)-(-)-carvone and (1R)-(-)-myrtenal by whole-cells of non-conventional yeasts (NCYs) belonging to the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma and Wickerhamomyces was studied. Volatiles produced were sampled by means of headspace solid-phase microextraction (SPME) and the compounds were analysed and identified by gas chromatography-mass spectroscopy (GC-MS). Yields (expressed as % of biotransformation) varied in dependence of the strain. The reduction of both (4R)-(-)-carvone and (1R)-(-)-myrtenal were catalyzed by some ene-reductases (ERs) and/or carbonyl reductases (CRs), which determined the formation of (1R,4R)-dihydrocarvone and (1R)-myrtenol respectively, as main flavouring products. The potential of NCYs as novel whole-cell biocatalysts for selective biotransformation of electron-poor alkenes for producing flavours and fragrances of industrial interest is discussed.

Background The ability of some microorganisms to accumulate lipids is well known; however, only recently the number of studies on microbial lipid biosynthesis for obtaining oleochemical products, namely biofuels and some building blocks for chemistry, is rapidly and spectacularly increased. Since 1990s, some oleaginous yeasts were studied for their ability to accumulate lipids up to 60–70% of their dry weight. Due to the vast array of engineering techniques currently available, the recombinant DNA technology was the main approach followed so far for obtaining lipid-overproducing yeasts, mainly belonging to the Yarrowia lipolytica. However, an alternative approach can be offered by worldwide diversity as source of novel oleaginous yeasts. Lipogenic aptitude of a number of yeast strains has been reviewed, but many of these studies utilized a limited number of species and/or different culture conditions that make impossible the comparison of different results. Accordingly, the lipogenic aptitude inside the yeast world is still far from being fully explored, and finding new oleaginous yeast species can acquire a strategic importance. Results Holtermanniella wattica, Leucosporidium creatinivorum, Naganishia adeliensis, Solicoccozyma aeria, and Solicoccozyma terricola strains were selected as a result of a large-scale screening on 706 yeasts (both Ascomycota and Basidiomycota). Lipid yields and fatty acid profiles of selected strains were evaluated at 20 and 25 °C on glucose, and on glycerol, xylose, galactose, sucrose, maltose, and cellobiose. A variable fatty acid profile was observed in dependence of both temperature and different carbon sources. On the whole, L. creatinivorum exhibited the highest performances: total lipid yield (YL) >7 g/l on glucose and glycerol, % of intracellular lipids on cell biomass (YL/DW) >70% at 20 °C on glucose, lipid coefficient (YL/Glu) around 20% on glucose, and daily productivity (YL/d) on glucose and sucrose >1.6 g/(l*d). Conclusions This study provides some meaningful information about the lipogenic ability of some yeast species. Variable lipid yields and fatty acid profiles were observed in dependence of both temperature and different carbon sources. L. creatinivorum exhibited the highest lipogenic performances. Electronic supplementary material The online version of this article (doi:10.1186/s13068-016-0672-1) contains supplementary material, which is available to authorized users.

Βeta-thalassemia, is a hemoglobinopathy characterized by reduced beta-globin chain synthesis, leading to imbalanced globin chain production, ineffective erythropoiesis and anemia. Increasing gamma-globin gene expression is a promising therapeutic approach as it reduces this imbalance by combining with the excess alpha globin chains and producing fetal hemoglobin (HbF). Furthermore, increased iron absorption and repeated blood transfusions lead to iron overload and tissue damage secondary to reactive oxygen species. Compounds exhibiting both antioxidant and HbF inducing activities are, therefore, highly desirable therapeutic agents. Resveratrol, a natural phytoalexin, combines these two activities but is also cytotoxic. Nine hydroxystilbenic resveratrol derivatives were synthesized in an attempt to identify compounds that retain the HbF-inducing and antioxidant activities of resveratrol but exhibit reduced cytotoxicity. Three derivatives (P1, P4 and P11) exhibited similar hemoglobin-inducing properties to resveratrol in K562 cells, however, only P11 showed reduced cytotoxicity. All three derivatives demonstrated variable HbF-inducing activity in primary erythroid progenitor cells from healthy donors. Resveratrol and P11 increased HbF induction significantly, with P11 having the highest activity. Additionally, P4 significantly increased progenitor numbers. A combinatorial treatment in K562 cells using resveratrol and decitabine resulted in a statistically significant increase in hemoglobin-inducing activity only above the level shown by resveratrol alone.

This work focuses on treatment of lead polluted waste glass from urban collection (Pb≈5% wt) for its valorization as support in the immobilization of lipase, because soda-lime glasses are used in enzymatic catalysis. First of all, an extraction process of surface Pb was performed. The process is based on nitrilotriacetic acid (NTA) chelating agent. The operating conditions are: T=80°C, t=1h, pH 10, solid/liquid weight ratio=1/10, reagent concentration=0.1 M. Leaching tests at controlled pH performed on treated glass confirmed the typical inert nature of the glass and the effectiveness of the NTA treatment. Afterwards lead polluted waste glasses, both untreated and treated with NTA, together with a commercial reference glass, were used as physical supports for the adsorption of Rhizopus oryzae lipase, in order to investigate their immobilization capability after specific washing/activation treatments to make functional their surface. The biocatalytic activity of the immobilized enzyme on the glasses was tested through the hydrolysis of para-nitrophenyl acetate to para-nitrophenol, quantifying the conversion percentage after 60 min of reaction by means of UV analyses at 410 nm. From the first results, lipase immobilization on waste glasses appears to be encouraging. In particular, lipase immobilized on lead polluted glass from urban collection showed an activity comparable to that of reference glass

Response surface methodology was applied in optimizing the asymmetric bioreduction of (4S)-(+)-carvone to dihydrocarvone (with low incidence of unsought side reactions) by using whole-cells of Cryptococcus gastricus. A factorial design (2(5)) including five independent variables was performed: X(1)=incubation time; X(2)=pH; X(3)=amount of whole-cells; X(4)=concentration of (4S)-(+)-carvone; X(5)=concentration of cofactor-recycling system. The utilization of glucose and glycerol as cofactor-recycling systems was checked. On the basis of the results of factorial design, three independent variables (X(1), X(3) and X(4)) out of five were further selected for performing a central composite design (CCD). First and second order polynomial equations obtained by CCD were used to select the optimal values of independent variables in order to maximize the bioreduction yield of (4S)-(+)-carvone and, at the same time, to minimize the occurrence of side reactions (i.e. further reduction of dihydrocarvone to dihydrocarveol).