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3 results on '"Luca, Elisa De"'

1. Use of Innovative (Micro)Extraction Techniques to Characterise Harpagophytum procumbens Root and its Commercial Food Supplements

Author

Diuzheva, Alina, Carradori, Simone, Andruch, Vasil, Locatelli, Marcello, Luca, Elisa De, Tiecco, Matteo, Germani, Raimondo, Menghini, Luigi, Nocentini, Alessio, Gratteri, Paola, and Campestre, Cristina

Subjects
Liquid Phase Microextraction, Plant Science, Sodium Chloride, DLLME, Harpagophytum procumbens, IL, MAE, NADES, Analytical Chemistry, Food Science, Biochemistry, Molecular Medicine, Drug Discovery3003 Pharmaceutical Science, Complementary and Alternative Medicine2708 Dermatology, Plant Roots, Phenols, Limit of Detection, Harpagophytum, Glycosides, Microwaves, Chromatography, High Pressure Liquid, Pyrans, Water, 2-Hydroxypropyl-beta-cyclodextrin, Glucose, Italy, Dietary Supplements, Solvents
Abstract

For the determination of harpagoside and the wide phenolic pattern in Harpagophytum procumbens root and its commercial food supplements, dispersive liquid-liquid microextraction (DLLME), ultrasound-assisted DLLME (UA-DLLME), and sugaring-out liquid-liquid extraction (SULLE) were tested and compared.In order to optimise the extraction efficiency, DLLME and UA-DLLME were performed in different solvents (water and aqueous solutions of glucose, β-cyclodextrin, (2-hydroxypropyl)-β-cyclodextrin, sodium chloride, natural deep eutectic solvent, and ionic liquid).The plant material was ground and sieved to obtain a uniform granulometry before extraction. Commercial food supplements, containing H. procumbens are commercially available in Italy.The most effective sodium chloride-aided-DLLME was then optimised and applied for analyses followed by HPLC-PDA. For comparison, microwave-assisted extraction was performed using the same solvents and the best results were obtained using 1% of β-cyclodextrin or 15% of sodium chloride.All commercial samples respected the European Pharmacopoeia monograph for this plant material, showing a harpagoside content ≥ 1.2%. Copyright © 2017 John WileySons, Ltd.

Published
2017

3. Cytotoxic and Enzyme Inhibitory Potential of Two Potentilla species (P. speciosa L. and P. reptans Willd.) and Their Chemical Composition.

Author

Uysal, Sengul, Zengin, Gokhan, Locatelli, Marcello, Bahadori, Mir B., Mocan, Andrei, Bellagamba, Giuseppe, Luca, Elisa De, Mollica, Adriano, and Aktumsek, Abdurrahman

Subjects
ENZYME inhibitors, CINQUEFOILS, DRUG development
Abstract

In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients. [ABSTRACT FROM AUTHOR]

Published
2017
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