Search

The Japanese threadfin bream N. japonicus (Bloch) (Perciformes: Nemipteridae) is an important marine food fish in Asia. However, our present knowledge of the occurrence of its nematode parasites is still limited. In the present study, the species composition and infection rate of ascaridoid nematodes in N. japonicus from the South China Sea, were studied for the first time. Five ascaridoid species, namely Anisakis typica (L3), Hysterothylacium amoyense (L3), Hysterothylacium sp. IV-A (L3), adult of H. thalassini and Raphidascaris lophii (L3), were identified using integrative taxonomy. Hysterothylacium amoyense was the most prevalent species (prevalence 47.2%, mean intensity 14.9 ± 17.1). Hysterothylacium thalassini and R. lophii were reported in the Japanese threadfin bream for the first time. Two different genotypes of A. typica (overall prevalence of 3.4%; mean intensity 1.7 ± 0.9) were found in the South China Sea for the first time. The unique restriction polymorphism patterns of three species of Hysterothylacium are provided for rapid diagnosis. Our present results indicate that RFLP analysis of ITS region, using the restriction enzymes HhaI and RsaI, represents a simple and practical method for large-scale surveys of Hysterothylacium for seafood industry.

Seuratascaris numidica (Seurat, 1917) is a specialized nematode species parasitizing amphibians only. In the present study, the detailed morphology of this poorly known species was studied using light and scanning electron microscopy based on the newly material collected from Hoplobatrachus chinensis (Osbeck) (Amphibia: Anura) in China. We found that the relative length of intestinal caecum in our male specimens (representing 68.4–71.1% of oesophageal length) is slighter longer than the previously reported data (not over 60.0% of oesophageal length). Our SEM observations also revealed the presence of ca. 64–76 small conical denticles on each lip. In addition, Angusticaecum wuyiensis Wang, 1981, collected from Rana schmackeri Boettger (Amphibia: Anura) from Wuyi Mountain in Fujian Province, China was considered as a new synonym of S. numidica. The ITS and cox1 sequences of S. numidica were also sequenced for the first time and there is no nucleotide variability detected in both regions. The present supplementary morphological and molecular data (especially the ITS and cox1 sequences) obtained herein is extremely important and useful to determine the morphological variability, population genetics and phylogenetic position of S. numidica in the future.

Sulfur was impregnated into hyper-cross-linked porous polymer (HCP) with a high specific area and unique porous structure. Compared to its inorganic or carbon counterparts, the HCP has a relatively high specific surface area of 1980 m2 g–1 with a total pore volume of 2.61 cm3 g–1, resulting in sulfur content in HCP/S of as high as 80 wt %. As a benefit of the unique HCP structure, the HCP/S composite exhibits a high initial discharge specific capacity (1333 mA h g–1 at 0.2 C), high-rate property, and good cycling stability (658 mA h g–1 after 120 cycles at 0.5 C and 604 mA h g–1 after 80 cycles at 1 C). Furthermore, the capacity of cells loses less than 1% after the first 20 charge/discharge cycles, while the HCP/S cathode can be cycled with an excellent Coulombic efficiency of above 94% after 120 cycles. Compared with pristine sulfur, the superior electrochemical performance of HCP/S composite is related to the cross-linked porous framework. Such structure could provide short ionic/electronic conduction ...

A series of antibodies against vascular endothelial growth factor (VEGF) have been developed for the treatment of various types of cancer, including non-small cell lung cancer (NSCLC) in recent years. However, tumors frequently demonstrate resistance to these strategies of VEGF inhibition. Efforts to better understand the mechanism underlying the acquired resistance to anti-VEGF antibodies are warranted. In the present study, in order to develop a xenograft model of acquired resistance to anti-VEGF antibody, xenografts of human adenocarcinoma A549 cells were generated through the successive inoculation of tumor tissue explants into first (F1), second (F2) and third (F3) generations of mice treated with the anti-VEGF antibody B20. Tumor growth rate and vessel-forming ability, assessed via cluster of differentiation (CD) 31 staining, were significantly lower in the F1, F2 and F3 groups compared with in the F0 control group (P

Species of Cosmocerca Diesing, 1861 (Ascaridomorpha: Cosmocercoidea), are common nematode parasites of amphibians. In the present study, a new species of Cosmocerca, namely C. simile n. sp., was described using light and scanning electron microscopy, and sequencing different nuclear and mitochondrial genetic markers (i.e. small ribosomal DNA (18S), large ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)). Cosmocerca simile n. sp. differs from its congeners based on body size, morphology and number of plectanes, relative length of spicules and gubernaculum and spicules to total body length and morphology and length of tail. Molecular analysis showed no nucleotide polymorphisms among different individuals of the new species regarding nuclear DNA. Very low intraspecific nucleotide variation (0.52-0.78%) was detected in cox1 mtDNA. In contrast, the level of interspecific nucleotide variation between C. simile n. sp. and its congeners were distinctly higher (2.74-18.1% in the partial ITS region and 10.2-13.5% in the partial cox1 region, respectively) than that of intraspecific variation. Phylogenetic analyses using maximum likelihood (ML) inference based on the partial ITS and cox1 sequence data both supported the new species to be a member of the genus Cosmocerca, and formed a sister relationship to C. japonica. The newly obtained genetic data are important for further studies of DNA-based taxonomy, population genetics and phylogenetics of the Cosmocercoidea.

Species of Megalobatrachonema Yamaguti, 1941 (Ascaridida: Cosmocercoidea) are important nematode parasites in amphibians and reptiles. However, the phylogenetic relationship of its included two subgenera Megalobatrachonema and Chabaudgolvania remains unclear. In the present study, a new species of Megalobatrachonema, M. (Chabaudgolvania) wangi sp. nov., was described based on the specimens collected from the lesser spiny frog Quasipaa exilispinosa (Liu & Hu) (Amphibia: Anura) in China. The ribosomal [large ribosomal DNA (28S) and internal transcribed spacer (ITS1–5.8S-ITS2)] and mitochondrial [12S small subunit ribosomal DNA and cytochrome c oxidase subunit 1 (cox1)] target regions of the new species and M. (Chabaudgolvania) terdentatum, together with the 12S region of M. (Megalobatrachonema) hainanensis, were amplified and sequenced for molecular identification and phylogeny. Moreover, in order to clarify the systematic position of the new species and the phylogenetic relationship of the two subgenera Megalobatrachonema and Chabaudgolvania, phylogenetic analyses based on 28S + ITS1–5.8S-ITS2 + 12S sequence data were performed using maximum likelihood (ML) inference and Bayesian inference (BI). The molecular phylogenetic results conflicted with the current classification and challenged the validity of the subgenus Chabaudgolvania, that should be a synonym of the subgenus Megalobatrachonema. The presence or absence of valves in the oesophageal bulb as a key criterion for delimitation of the two subgenera Megalobatrachonema and Chabaudgolvania seems to be unreliable.

Subulura chinensis Schwartz, 1926 is a hitherto poorly known nematode species. The morphology of S. chinensis has not been sufficiently well described. In addition, the molecular data from species of the Subuluroidea are extremely limited. The detailed morphology of S. chinensis was studied using light microscopy and, for the first time, scanning electron microscopy, based on newly collected specimens from the little owl Athene noctua (Scopoli) (Strigiformes: Strigidae) in China. The internal transcribed spacer (ITS) of ribosomal DNA and mitochondrial cytochrome c oxidase subunit 1 (cox1) target regions of S. chinensis were first amplified by polymerase chain reaction (PCR), then sequenced and analysed for the molecular identification of this species. Our SEM observations showed for the fist time the detailed morphology of the cephalic extremity, precloacal pseudo-sucker, caudal papillae, gubernaculum, phasmids and vulva of S. chinensis, and also determined the presence of a small, single medio-ventral precloacal papilla in the male. Moreover, we detected the presence of 0.08–0.40% and 0–1.30% nucleotide divergence among different individuals of S. chinensis in the ITS and cox1 regions, respectively. The supplementary morphological characters and genetic data will be very useful for the diagnosis of this poorly known species.

To describe the fertility outcomes after hysteroscopic adhesiolysis combined with preoperative hormone treatment in women with intrauterine adhesion (IUA). This study is a retrospective cohort study. A total of 230 patients with IUA underwent hysteroscopic adhesiolysis combined with hormone treatment from Jan 2012 to Jun 2018. 148 patients who received preoperative estrogen treatment were enrolled into group A and 82 patients without preoperative estrogen treatment were enrolled into group B. All the patients underwent hysteroscopic adhesiolysis and received postoperative estrogen therapy, intrauterine indwelling device. Second or third look was performed after 2–3 months. 90.87% (209/230) patients complete the study. The AFS scores at baseline were higher in the group A than group B. After the preoperative E2 treatment, group A achieved the comparable AFS score to group B before the surgery. The cumulative fertility rate in group A was comparable in group B, both in the ITT analysis (49.32% vs. 52.44%, p = 0.651) and the PP analysis (54.07% vs.58.10%, p = 0.575).The mean conception time was also similar in group A and group B (8.30 ± 6.47 vs. 8.54 ± 5.68 months, p = 0.837). Besides, the surgery times in group A were less than group B. There was no difference in the rate of adverse events between two groups. Hysteroscopic adhesiolysis combined with preoperative oestrogen could reduce the preoperative AFS scores and the times of surgery which yield a similar conception rate in women with less severe intrauterine adhesions.

Marine fishes represent the important components of the diet in the coastal areas of China and they are also natural hosts of various parasites. However, to date, little is known about the occurrence of ascaridoid parasites in the frequently consumed marine fishes in China. In order to determine the presence of ascaridoid parasites in the frequently consumed marine fishes in the coastal town Huizhou, Guangdong Province, China, 211 fish representing 45 species caught from the South China Sea (off Daya Gulf) were examined. Five species of ascaridoid nematodes at different developmental stages were detected in the marine fishes examined herein, including third-stage larva of Anisakis typica (Diesing, 1860), third and fourth-stage larvae of Hysterothylacium sp. IV-A of Shamsi, Gasser & Beveridge, 2013, adult and third-stage larvae of Hysterothylacium zhoushanense Li, Liu & Zhang, 2014, adults and third-stage larvae of Raphidascaris lophii (Wu, 1949) and adults of Raphidascaris longispicula Li, Liu & Zhang, 2012. The overall prevalence of infection is 18.0%. Of them, Hysterothylacium sp. IV-A with the highest prevalence (17.5%) and intensity (mean=14.6) of infection was the predominant species. The prevalence and intensity of A. typica were very low (1/211 of marine fish infected with an intensity of one parasite per fish). The morphological and molecular characterization of all nematode species was provided. A cladistic analysis based on ITS sequence was constructed in order to determine the phylogenetic relationships of these ascaridoid parasites obtained herein. The present study provided important information on the occurrence and diagnosis of ascaridoid nematodes in the commercially important marine fishes from the South China Sea. The low level of infection and the species composition of ascaridoid nematodes seem to indicate the presence of low risk of human anisakidosis when local population consumed these marine fishes examined herein.

The genus Megalobatrachonema is a rare group of nematode parasites within Ascaridida. The systematic status of Megalobatrachonema in the superfamily Cosmocercoidea (Ascaridida) has long been controversial. The relationship of Megalobatrachonema and Chabaudgolvania remains unsolved. In the present study, a new species of Megalobatrachonema, M. hainanensis sp. nov., was described based on specimens collected in Amolops hainanensis (Boulenger) and Hylarana spinulosa (Smith) (Amphibia: Anura) from Hainan Island, China. The large ribosomal DNA (28S) and internal transcribed spacer (ITS1-5.8S-ITS2) were also sequenced for molecular identification and phylogenetic study. Phylogenetic analyses using maximum likelihood (ML) inference and Bayesian inference (BI) based on 28S and ITS1 sequence data, respectively, supported that Megalobatrachonema is a member of the family Kathlaniidae. In addition, the genetic comparison and phylogenetic results based on ITS1 sequence data also supported that the genus Chabaudgolvania should be considered as a synonym of Megalobatrachonema.

A new species of nematode,Orientatractis longicaudatan. sp. is described from the intestine ofIndotestudo elongata(Blyth) (Testudinidae) from Zoo of Tianjin, Tianjin, China. The new species can be easily distinguished from its congers by having longer tail, by the length of gubernaculum and left spicule, and by the numbers of caudal papillae. This is first species ofOrientatractisPetter, 1966 reported in China. A key to the species ofOrientatractisis provided.

A catalogue, based on both examined specimens and the published literature, of all the ascaridoid nematodes recorded in China is presented. A total of 95 recognised species, representing 26 genera in five families, are reported. Detailed information on the type-host, type-locality, original reference, synonyms, annotated subsequent references of taxonomic importance, other host records, site of infection, location of type-specimens and distribution are listed for each recognised species. Additional comments on the taxonomic status of some species are also given. Moreover, some nomenclatural changes are proposed: (i) Toxascaris selenarctis Wang, 1965 and T. ailuri Wu, He & Hu, 1987 are placed in synonymy with Baylisascaris transfuga (Rudolphi, 1819); (ii) Raphidascaris lophii Wang & Wu, 1991 is a secondary homonym of R. lophii (Wu, 1949) and a replacement name, R. wangi nom. nov., is proposed for the former species; (iii) Aliascaris aetoplatea Luo, 2001 is transferred to Terranova Leiper & Atkinson, 1914, as T. aetoplatea (Luo, 2001) n. comb., and should be considered a species inquirenda; (iv) Ophidascaris orientalis (Wang, 1965) is resurrected as a valid species; (v) Phocascaris longispiculum Wang & Wu, 1991 and Ophidascaris agkistrodontis Wang, 1979 are treated as incertae sedis; and (vi) Hysterothylacium sauridae Li, Xu & Zhang, 2008 is listed as a nomen nudum.

SnO2 shells with micromesopores are synthesized using the template sacrifice method from silica sphere templates. The pores and specific surface area are characterized with SEM, TEM, and BET absorptions. Sulfur is introduced into the SnO2 shells up to 66 wt % according to TGA results. Extra sulfur can only be located at the outer surface of SnO2, resulting in a drastically reduced specific surface area. Because of the unique structure, the S/SnO2 composites with 66 wt % sulfur content exhibit a high initial capacity of 1517 mA·h·g–1 at a current density of 0.2 C, and 1176 mA·h·g–1 at 0.5 C, and remaining capacity of 1176 and 736.6 mA·h·g–1 after 50 cycles, respectively. The performance is much better than that of pure sulfur or S/SnO2 at higher sulfur content. Better performance of S/SnO2 at 66 wt % is attributed to the micromesopores and the shell framework of SnO2, while the performance fading at higher sulfur content is owing to the coating of extra sulfur on the outer surface of SnO2 shells.

The starry batfish Halieutaea stellata (Vahl) is a small, benthic fish found in Indo-West Pacific Oceans. However, our present knowledge of the helminth parasites of this fish is still fragmentary. In this study, a total of 29 fish collected from the East and South China Sea were examined to determine the prevalence, intensity and species composition of helminth parasites in H. stellata. Using morphological and molecular approaches, four species of nematodes were found parasitic in this fish host, including the adults and fourth-stage larvae of Raphidascaroides nipponensis Yamaguti 1941; adults and third-stage larvae of Raphidascaris lophii (Wu 1949), third- and fourth-stage larvae of Hysterothylacium larval type IV-A of Shamsi, Gasser & Beveridge 2013 and third-stage larvae of Hysterothylacium amoyense (Hsu 1993). Halieutaea stellata represents a new host record for the three last-named nematodes. Raphidascaroides nipponensis with the highest prevalence (82.5%) and intensity (mean = 13.5) of infection was considered as the dominant parasite species in H. stellata. The detailed morphology of the different developmental stages of the four nematode species was studied using light and scanning electron microscopy. All nematode species were also genetically characterized by sequencing and analysing the internal transcribed spacer (ITS) of the ribosomal DNA. This study provides further data on the occurrence of nematode parasites in H. stellata and also contributes to facilitate an accurate and rapid diagnosis of the infection by these little-known nematodes.

A new cosmocercid species, Cosmocercoides qingtianensis sp. n., collected from the intestine of the Asiatic toad Bufo gargarizans Cantor (Amphibia: Anura) is described using integrated approaches, including light and scanning electron microscopy, and sequencing and analyzing the ribosomal [small ribosomal DNA (18S) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions, respectively. The new species can be distinguished from its congeners by the combination of the following morphological characters, including the large body size, the presence of lateral alae and somatic papillae in both sexes, the length of spicules, the particular morphology and length of gubernaculum, the number, arrangement and morphology of caudal rosettes, the presence of large medioventral precloacal papilla and the long tail. Our molecular analysis revealed the level of intraspecific genetic variation of C. qingtianensis sp. n. distinctly lower than that of the interspecific genetic variation in the ITS and cox1 regions. However, there are some overlaps in the range of intra- and interspecific 18S sequence divergence between the new species and some closely related species. The results of molecular analysis supported the validity of the new species based on the morphological observations. The 18S, ITS, and cox1 regions of C. pulcher collected from Bufo japonicus formosus in Japan were also sequenced and analyzed. The results showed a low level of intraspecific genetic variation in 18S and ITS regions (0-0.12% and 0-0.23% nucleotide differences, respectively), but a relatively high level of intraspecific genetic variation in cox1 region (0.78-4.69% nucleotide differences). In addition, it seems more powerful and practical to use the cox1 region as a genetic marker for the accurate identification and differentiation of species of Cosmocercoides than the 18S and ITS regions, especially for the closely related species.

To obtain microRNA (miRNA) profile and clarify their biological function in tumorigenic Sca-1(+) CD34(+) cells during carcinogenesis of lung adenocarcinoma. After intranasal infection with recombinant Adeno-Cre viruses (AdV-Cre), lung adenocarcinoma was identified pathologically in Lox-stop-lox Kras (LSL-Kras) G12D mice. Sca-1(+) CD34(+) cells were sorted by flow cytometry and tested for tumor-initiating ability, self-renewal and tumorigenicity. MiRNA profiles were obtained using microarray and further confirmed by real-time RT-PCR (qRT-PCR). MiRNA functions were predicted bioinformatically, and miR-294 function was verified to explore its role in tumor migration and invasion. Lung adenocarcinoma was induced in LSL-Kras G12D mice within 30 days. In vivo, the tumorigenicity of Sca-1(+) CD34(+) cells was 25 times stronger than Sca-1(-) CD34(-) cells. During tumorigenesis of lung adenocarcinoma, the expression of 145 miRNAs in Sca-1(+) CD34(+) cells increased and 72 miRNAs decreased (P < 0.01). Four successively up-regulated miRNAs (miR-15a*, miR-203, miR-294 and miR-295*) and three successively down-regulated ones (miR-19b, miR-483 and miR-615-5p) were identified. Among them, miR-294 could constitutively bind to 3'-UTR of matrix metalloproteinase 3 (MMP3), and down-regulate MMP3 protein expression. MiR-294 also significantly inhibited migration and invasion of Lewis lung cancer cells. MiRNAs are characteristically expressed in tumor-initiating Sca-1(+) CD34(+) cells of lung adenocarcinoma, and may play important roles during the carcinogenesis of lung adenocarcinoma.

Ascaridoids are among the commonest groups of zooparasitic nematodes (roundworms) and occur in the alimentary canal of all major vertebrate groups, including humans. They have an extremely high diversity and are of major socio-economic importance. However, their evolutionary history remains poorly known. Herein, we performed a comprehensive phylogenetic analysis of the Ascaridoidea. Our results divided the Ascaridoidea into six monophyletic major clades, i.e., the Heterocheilidae, Acanthocheilidae, Anisakidae, Ascarididae, Toxocaridae, and Raphidascarididae, among which the Heterocheilidae, rather than the Acanthocheilidae, represents the sister clade to the remaining ascaridoids. The phylogeny was calibrated using an approach that involves time priors from fossils of the co-evolving hosts, and dates the common ancestor of the Ascaridoidea back to the Early Carboniferous (approximately 360.47-325.27 Ma). The divergence dates and ancestral host types indicated by our study suggest that members of the Ascaridoidea first parasitized terrestrial tetrapods, and subsequently, extended their host range to elasmobranchs and teleosts. We also propose that the fundamental terrestrial-aquatic switches of these nematodes were affected by changes in sea-level during the Triassic to the Early Cretaceous.

This paper developed a composite indicator for evaluating the performance of low-carbon economy for the cities in Jiangsu province, China. The empirical results show that the performance of low carbon economy of cities in Jiangsu province may be divided into three levels. The first level include Yangzhou, Zhenjiang, and Nanjing, that the performance is below the province average level; the second level including Huaian, Suqian, Lianyungang, and Nantong which, that the performance is similar with the province average level; the rest of the cities in Jiangsu are set as third level. At same time, this paper analyze some cities’ performance of low carbon economy.

Online long-term tracking is a challenging problem as data streams change over time. In this paper, sparse representation has been applied to visual tracking by finding the most correct sample with minimal reconstruction error using compressed Haar-like features. However, most sparse representation tracking algorithm introduce l1 regularization into the PCA reconstruction using samples directly, which leads to complexity computation and can not adapt to occlusion, rotation and change in size. Our model updating not only uses the samples from the training set, but also generates the warped versions (include scale variation, rotation, occlusion and illumination changes) for the previous tracking result. Also, we do not use the samples in models for sparse representation directly, but the Haar-like features instead which are compressed in a very low-dimensional space. In addition, we use a robust and fast algorithm which exploits the spatio-temporal context for predicting the target location in the next frame. This step will lead to the reduction of the searching range by the detector. We demonstrate the proposed method is able to track objects well under pose and scale variation, rotation, occlusion and illumination with great real-time performance on challenging image sequences.

Objective To establish a modified method, mutant-enriched PCR combined with pyrosequencing, for the detection of K-ras mutations in plasma and cancer tissue of patients with NSCLC. Methods The cancer tissue and matched blood samples were collected and DNA was extracted from 43 NSCLC patients. The mutations of codon 12 and 13 in expressed region 2 of K-ras gene were detected by the established mutant-enriched PCR-pyrosequencing method. Cell line possessing mutant type/wild type of K-ras gene (A549/HCC827) was used to evaluate the sensitivity of the newly developed method. Results Four K-ras mutations were detected from cancer tissue samples (4/43, 9.3%), and 3 mutations were detected from plasma samples (3/43, 7.0%), all the mutations were 12 codon mutation. The consistency of K-ras mutation observed in both plasma and its matched tissue was 75%, and the sensitivity of the newly developed method was 0.1%. Conclusion The newly developed mutant-enriched PCR-pyrosequencing method has such advantages as rapid, accurate and high sensitivity. So it may be used for clinical screening of K-ras gene mutation in NSCLC patients. DOI: 10.11855/j.issn.0577-7402.2014.03.07

A variable scale compressive tracking algorithm based on structural constraint sample is presented to solve the variable scale problem in this paper. A number of scanning windows with different scales and positions are obtained by structural constraint sampling.Some sparse random sensing matrices with different scales that can be computed offline easily are adopted to extract the features of different foreground target and background sample image patches with relevant scales online, the sample patch having a maximal score is regarded as the new tracking result by classifying the compressive features via a naive bayesian classifier,meanwhile,to update the location and scale. Experimental results show the proposed algorithm performs favorably against state-of-the-art algorithms on challenging sequences in terms of the basic attitude and scale change, which is robust and does not depend on the scale selection of the initial tracking area.

The detection of small moving target in the context of complex background is a difficult issue. A method combining interframe differential registration and adaptive wiener filtering aimed to suppress background to detect moving object in complex background is proposed. The fixed background in the fore-and-aft frames can be filtered out by the interframe registration which preserves the moving target, parts of background and noise due to interframe movement and the gray-scale fluctuation. On one hand the complex background is estimated by an adaptive wiener filter, and the background suppression leaves the high-frequency regions containing the moving target in image. On the other hand, most of the high-frequency regions corresponding to non-target area are eliminated by the inter-frame registration in the differential images. The motion of target is continual in image sequences, while the position of the leaked background is relatively fixed and the noise is of small size. The fusion of the background suppression and inter-frame registration makes the discrimination of targets, background and noise possible. The small moving target is detected by trajectory association based on its interframe trajectory continuity. Experiment results verify the feasibility of the method.

A new detecting algorithm based on gray-weighted kernel function is proposed for the moving target detection (MTD) in dynamic series of image.This algorithm firstly realizes image sequence registration by using the biggest gradient point. Then divides the image into 32*32 sub-images. The moving target can be finally detected based on the changes of gray-weighted kernel function. The testing result shows that the algorithm can detect the moving target in real-time and can suppress the influence caused by image registration error and gray fluctuation effectively.

Fund is one of the key elements for Energy Industry to make a development aiming at low-carbon emission. This paper analyzes the connotation of low-carbon emission for Energy Industry and principles of financial support for the Energy Industry of low-carbon development, pointing out the short-coming of the current financial support for the development of China 's Energy Industry to achieve low carbon emission, and recommending the ways to enhance the current financial support such as financing, financial innovation, and cooperation of banking enterprises, etc.

From 35 species of marine fishes (n = 327) from the South China Sea, 237 nematode larvae were collected and identified morphologically as Anisakis. Genomic DNA was isolated from each larva and subjected to PCR-based RFLP and targeted sequencing of a nuclear ribosomal DNA region between the 3'-end of the small subunit and 5'-end of the large subunit of the rRNA genes (= internal transcribed spacers, ITS+). Four different RFLP profile combinations (sets) were detected for all restriction endonucleases (HinfI, HhaI, and TaqI), of which three were characteristic of Anisakis typica, A. pegreffii, and A. physeteris, respectively. One profile set (for sample CA-2012) was linked to an ITS+ sequence that was identical to a previously published sequence of Anisakis sp. (sample HC-2005; originating from the African shelf) and another sequence (PH-2010; Madeira, Portugal). Phylogenetic analysis was carried out using the ITS+ sequence data from this study and reference sequences from the GenBank database. Neighbor joining and maximum parsimony trees displayed three clades. Clades I and II included nine described species of Anisakis, including all type I and type II larvae; clade III represented some undescribed species of Anisakis. Morphological comparison showed that Anisakis sp. CA-2012 was distinct from type I and type II larvae based on its tail shape and ratio of tail length to body length. The phylogenetic analysis and morphological characters suggest that Anisakis sp. CA-2012 represents a new record, now called Anisakis type III larvae.

Heliconema hainanensis sp. nov. collected from Uroconger lepturus (Richardson) (Anguilliformes: Congridae), Muraenesox cinereus (Forsskål) and Congresox talabonoides (Bleeker) (Anguilliformes: Muraenesocidae) in the South China Sea was described using light and scanning electron microscopy. The new species differs from its congeners by the following morphology: pseudolabia, the number and arrangement of caudal papillae (4 pairs of pedunculate precloacal papillae arranged in 2 groups of 2 and 2 pairs and 6 pairs of pedunculate postcloacal papillae arranged in 4 groups of 1, 2, 1 and 2 pairs), the length of spicules [left spicule 0.51-0.69 mm, right spicule 0.20-0.27 mm, spicule (right:left) ratio 1:2.20-2.69] and the morphology of the female tail tip. In addition, specimens of the new species collected from the three different hosts and specimens of an unidentified species of Heliconema collected from U. lepturus were characterised using molecular methods by sequencing the internal transcribed spacer (ITS) of ribosomal DNA. Analyses and comparison of the ITS sequence of H. hainanensis sp. nov. with Heliconema sp. support the validity of the new species based on morphological observations. An identification key to the species of Heliconema is also provided.

Durettenema guangdongense gen. et sp. nov. is described from Hipposideros larvatus (Horsfield) (Chiroptera: Rhinolophidae) in Guangdong Province, China. The new genus differs from the other genera of subfamily Molineinae in the structure of the synlophe, the absence of lateral alae, the arrangement of the bursa rays, the shape of the spicules, the female tail and the presence of gubernaculum. Meanwhile, considering the morphological characters of Macielia rhinolophi Yin, 1980, including the pattern of the bursa ray, the shape of the spicules, and the female tail, this species should be transferred to the genus Durettenema, as D. rhinolophi (Yin, 1980) comb. nov., which can be distinguished from D. guangdongense in the shape of the ovejector. In addition, the ITS-1 sequences of D. guangdongense were also analysed, these sequence added new data for the molecular diagnosis of trichostrongylid nematodes.

Habronematid nematodes were collected from the stomachs of donkeys, Equus asinus L., in the Tarim Basin, Xinjiang, China. After examination by light and scanning electron microscopy, Habronema muscae (Carter, 1861) and H. majus (Creplin, 1849) were identified. The morphology of our specimens representing H. muscae (Carter, 1861) agreed well with previous redescriptions in the shape of the lateral lips, origin of the lateral alae, ratio of left and right spicules, and number and arrangement of caudal papillae. However, H. majus (Creplin, 1849) differs from H. microstoma (Schneider, 1866) in the arrangement of the caudal papillae in the male. Moreover, molecular analysis also showed interspecific differences of 26.2-28.2% in ITS2 and 8.6-8.9% in cox1 between H. majus and H. microstoma, a divergence much higher than the known intraspecific variation of Habronema spp. (6.6-8.7% in ITS2; 0.2-2.2% in cox1). The results indicate that both H. microstoma (Schneider, 1866) and H. majus (Creplin, 1849) are valid species.

Longicollum pagrosomi Yamaguti, 1935 (Acanthocephala: Pomphorhynchidae) collected from the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel) (Perciformes: Oplegnathidae) in the East China Sea (off Zhoushan Islands) was studied using light and scanning electron microscopy. The SEM observations revealed for the first time the presence of about 28 well-developed sensory papillae arranged in a circle on the copulatory bursa. In addition, L. pagrosomi was characterised using molecular methods by sequencing of the internal transcribed spacer (ITS) of the ribosomal DNA based on the newly collected material. Longicollum pagrosomi is the first species of the genus with the ITS region sequenced for the purpose of species identification. These new morphological and molecular data contributed to a reliable and accurate specific identification and differentiation of species.

Hysterothylacium zhoushanensis sp. nov. collected from the intestine of the flatfish Pseudorhombus oligodon (Bleeker) (Pleuronectiformes: Paralichthyidae) in the East China Sea is described and illustrated by light and scanning electron microscopy. The new species can be easily distinguished from its congeners by the presence of remarkable lateral alae, the very short intestinal caecum, the unusually long ventricular appendix (ratio of intestinal caecum to ventricular appendix 1:8.74-23.8), the short spicules (0.58-0.81 mm long, representing 1.70-2.08 % of body length) and the number and arrangement of male caudal papillae (35-42 pairs in total, arranged as 26-32 pairs of precloacal, two pairs of paracloacal and six to eight pairs of postcloacal). In addition, the adults and the putative third-stage larvae identified morphologically of the new species are characterised by sequencing and analysing the internal transcribed spacer (ITS) of the ribosomal DNA. The result reveals that they are homogeneous genetically, and all belong to the same species. Molecular analysis by comparing the ITS gene of H. zhoushanensis sp. nov. with these species of Hysterothylacium available in GenBank also seem to support the validity of the new species based on the morphological observation.

A new ascaridoid nematode Mawsonascaris zhoui sp. nov. collected from the granulated guitarfish Glaucostegus granulatus (Cuvier) (Rajiformes: Rhinobatidae) in the Taiwan Strait (off Xiamen, Fujian Province, China) is described and illustrated in this paper. The new species can easily be distinguished from its congeners by the absence of an intestinal caecum, the presence of a single, median precloacal volcaniform papilla, a pair of paracloacal double papillae and 10–11 precloacal papillae in the male. In addition, the paratypes of Raphidascaroides myliobatum Yin & Zhang, 1983 collected from the Japanese eagle ray Myliobatis tobijei Bleeker (Rajiformes: Myliobatidae) in the Taiwan Strait (off Chongwu, Fujian Province, China) were also re-examined and the morphology of the species reveals that it also belongs to the genus Mawsonascaris, as M. myliobatum (Yin & Zhang, 1983) comb. nov. An identification key to the species of Mawsonascaris is provided.

Goeziine nematodes identified as Goezia nankingensis Hsü, 1933 were collected from the Chinese paddlefish Psephurus gladius (Martens) (Acipenseriformes: Polyodontidae), a critically endangered freshwater fish, from the Yangtze River in China. Their examination, using light microscopy and, for the first time, scanning electron microscopy, revealed some erroneous and previously unreported morphological features, necessitating the redescription of this poorly known species. Goezia nankingensis is easily distinguished from its congeners by the morphology and arrangement of its cuticular spines, the short intestinal caecum and long ventricular appendix (ratio of intestinal caecum to ventricular appendix 1:10.0-18.3), the short spicules (0.34-0.49 mm long, representing 6.09-10.1% of the body length), the number and arrangement of male caudal papillae [14-16 pairs in total, approximately arranged: precloacal 7-10 pairs, paracloacal 2 pairs and postcloacal 4-6 pairs (one pair double)], and the presence of a particular medio-ventral, precloacal papilla in the male.

Nematodes of the family Anisakidae parasitize fish, mammals, birds and reptiles, with the larval stages of some species causing severe clinical disease in humans. Therefore, the accurate identification of anisakid nematodes is a key component of disease surveillance and control. An epidemiological survey of 123 fishes comprising eight different species from the Yellow Sea in China revealed that more than 25% of fish were infected with the larvae of anisakids, 200 third-stage larvae (L3s) were collected from fish and then subjected to morphological and molecular study. Larvae identified as Anisakis type I (n=197) and Hysterothylacium sp. (n=3), based on morphological criteria were characterized genetically by mutation scanning, followed by targeted sequencing of the first and second internal transcribed spacers of nuclear ribosomal DNA. Comparison of the sequences obtained from a subset of 27 specimens with those available in public gene databases showed that all samples identified morphologically as Anisakis type I (n=197) were Anisakis pegreffii, whereas those identified as Hysterothylacium sp. (n=3) were Hysterothylacium aduncum. The approach used herein was an effective means of matching incompletely identifiable larval nematodes with identifiable reference sequences, and provides a basis for exploring the composition of populations of anisakid larvae in fish as well as their ecology, particularly their life cycles and transmission patterns.

SummarySynhimantus (Dispharynx) mawsonae n. sp. is described from the boobook owl, Ninox boobook (Latham, 1802) (Aves: Strigidae), from New South Wales, Australia. The new species differs from congeners in the number and arrangement of pedunculate caudal papillae, length and shape of spicules, in having straight, only slightly sinuous cordons and in having tricuspid cervical papillae. Procyrnea dollfusi (Mawson, 1968) is redescribed from the type host, N. boobook; new features described are the imbricated cervical cuticle and the ornamentation of the tail of the male. Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) is also reported from N. boobook, confirming original records and extending its geographical range within Australia.

Ophidascaris excavata Hsu & Hoeppli, 1931 is a poorly known ascaridid parasite reported from the short-tailed pit viper Gloydius brevicaudus (Stejneger) (Reptilia: Viperidae) in China. In the present paper, the detailed morphology of this nematode was studied using light and scanning electron microscopy (SEM) based on newly collected material. The results revealed several important, but previously unreported, morphological features, including the presence of one pair of small, finger-like prolongations on each lip, narrow cervical alae beginning well posterior to the base of the ventrolateral lips and the second pair of postcloacal ventro-lateral papillae being double; in addition, there is no intestinal caecum. These supplementary morphological and morphometric data, especially the detailed morphological features obtained herein under SEM, would help us to understand the relationships of O. excavata with its congeners and enable us to diagnose this species more accurately.

Moyamoya disease (MMD) is a rare chronic occlusive cerebrovascular disease, with some non-typical symptoms including ischaemic stroke, transient ischaemic attack (TIA), intracranial haemorrhage, se...

Porrocaecum parvum n. sp. is described from the grey-faced buzzard Butastur indicus (Gmelin) (Accipitriformes: Accipitridae) in China. The new species differs from its congeners in having well-developed cervical alae, small interlabia and very short intestinal caecum (0.34 mm long, representing 11.9% of oesophageal length) and in the number and arrangement of the caudal papillae (29 pairs in total, arranged as follows: 21 pairs precloacal, single double pair paracloacal and seven pairs postcloacal) and in the morphology of the male tail. In addition, Porrocaecum reticulatum (Linstow, 1899), collected from the purple heron Ardea purpurea L., the grey heron A. cinerea L. and the little egret Egretta garzetta (L.) (Pelecaniformes: Ardeidae) in China, was also studied using light and, for the first time, scanning electron microscopy. Previously unreported and erroneous morphological features of taxonomic significance are revealed, including the presence of narrow cervical alae, single pair of small, submedial pores and single, short medial ditch on each lip, interlabia with very pointed anterior prolongation, single medio-ventral precloacal papilla on anterior cloacal lip and double paracloacal papillae slightly posterior to cloaca.

Ascaridia galli (Schrank, 1788) is a common parasite of various galliform birds worldwide. Although A. galli has been extensively studied by many author, knowledge of the morphology of this species in detail is still insufficient. In the present paper, the detailed morphology of A. galli was further studied using light and scanning electron microscopy, based on specimens collected from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae) in China. The results revealed some erroneous and previously unreported morphological features, including the lips lacking real denticles, the lateral alae beginning at some distance posterior to the base of the ventrolateral lips and the caudal papillae with 4 different morphotypes. The present morphological and morphometric data complement previous descriptions and enable us to recognize this species more precisely.

Ophidascaris wangi sp. n. collected from the king rat snake Elaphe carinata (Gunther) (Serpentes: Colubridae) in China is described using both light and scanning electron microscopy. The new species differs from its congeners in the presence of narrow lateral alae originating a short distance posterior to the base of the ventrolateral lips, its relatively long oesophagus (3.57-4.54 mm long, representing 6.6-7.6% of body length), its short spicules (1.89-2.14 mm long, representing 3.9-4.3% of body length), the number and arrangement of caudal papillae (49-57 pairs in total, arranged as follows: 43-51 pairs precloacal, 2 pairs joined paracloacal and 4 pairs postcloacal), the presence of a particular papilliform medioventral, postcloacal ornamentation and the morphology of the eggs and tip of the female tail. In addition, Ophidascaris najae (Gedoelst, 1916), collected from the king cobra Ophiophagus hannah Cantor (Serpentes: Elapidae) in China, is also redescribed. The morphology of the cervical papillae, labial denticles and phasmids of the female is described for the first time.

Philometra lagocephali Moravec et Justine, 2008 was redescribed using light and scanning electron microscopy based on the new material collected from the lunartail puffer Lagocephalus lunaris (Bloch et Schneider) (Tetraodontiformes: Tetradontidae) in the South China Sea. Our specimens are identical with the original description in the body size of gravid female, the width of cephalic and caudal end, the number and arrangement of cephalic papillae, the relative length of the oesophagus to body length, the size of ventriculus, the morphology of oesophageal bulb and posterior end, and the body size of larvae. In addition, specimens of P. lagocephali were also characterized using molecular methods by sequencing and analysing the small ribosomal DNA (18S rDNA) and mitochondrial cytochrome c coxidase subunit 1 (cox1) target regions. Phylogenetic analyses of genus Philometra base on 18S rDNA sequences was made using Mega 5.2. The results confirmed P. lagocephali is a valid species. It’s the first molecular study of this species. These new morphological and molecular data contributed to a more precise and rapid diagnosis of this hitherto poorly known philometrid nematode.