Search

This article, titled "[Retracted] MicroRNA-378 regulates cell proliferation and migration by repressing RNF31 in pituitary adenoma," was published in Oncology Letters in 2018. However, it has since been retracted due to concerns raised by a reader. The reader pointed out similarities between the scratch-wound assay data in Figure 3 of this article and data from a different paper submitted to the same journal. Additionally, there were possible anomalies in the "Control" panel. The editor decided to retract the paper due to a lack of confidence in the data and the authors did not provide an explanation. [Extracted from the article]

MicroRNA-378 (miR-378) is dysregulated in multiple malignancies and is associated with tumor progression. However, the expression and mechanism of miR-378 in pituitary adenoma (PA) remains to be elucidated. In the present study, the role and mechanism of miR-378 in PA tumorigenesis and development was investigated. It was revealed that the levels of miR-378 expression were markedly downregulated in PA tissues. CCK-8 and wound healing assays revealed that transfection with miR-378 mimics was able to markedly inhibit the proliferation and migration of GH3 cells. Furthermore, quantitative polymerase chain reaction analysis demonstrated that ring finger protein 31 (RNF31) was upregulated in PA specimens and the levels of RNF31 expression was negatively regulated by miR-378. In addition, knockdown of RNF31 markedly suppressed cell proliferation and migration in GH3 cells. In conclusion, the present study provides a molecular basis for the function of miR-378/RNF31 in the progression of human PA, indicating a potential novel target for the treatment of PA. [ABSTRACT FROM AUTHOR]

Objective Methods Results Conclusion As the population ages and technology advances, lateral lumbar intervertebral fusion (LLIF) is gaining popularity for the treatment of degenerative lumbar scoliosis (DLS). This study investigated the feasibility, minimally invasive concept, and benefits of LLIF for the treatment of DLS by observing and assessing the clinical efficacy, imaging changes, and complications following the procedure.A retrospective analysis was performed for 52 DLS patients (12 men and 40 women, aged 65.84 ± 9.873 years) who underwent LLIF from January 2019 to January 2023. The operation time, blood loss, complications, clinical efficacy indicators (visual analogue scale [VAS], Oswestry disability index [ODI], and 36‐Item Short Form Survey), and imaging indicators (coronal position: Cobb angle and center sacral vertical line–C7 plumbline [CSVL–C7PL]; and sagittal position: sagittal vertical axis [SVA], lumbar lordosis [LL], pelvic incidence angle [PI], and thoracic kyphosis angle [TK] were measured). All patients were followed up. The above clinical evaluation indexes and imaging outcomes of patients postoperatively and at last follow‐up were compared to their preoperative results.Compared to the preoperative values, the Cobb angle and LL angle were significantly improved after surgery (p < 0.001). Meanwhile, CSVL–C7PL, SVA, and TK did not change much after surgery (p > 0.05) but improved significantly at follow‐up (p < 0.001). There was no significant change in PI at either the postoperative or follow‐up timepoint. The operation took 283.90 ± 81.62 min and resulted in a total blood loss of 257.27 ± 213.44 mL. No significant complications occurred. Patients were followed up for to 21.7 ± 9.8 months. VAS, ODI, and SF‐36 scores improved considerably at postoperative and final follow‐up compared to preoperative levels (p < 0.001). After surgery, the Cobb angle and LL angle had improved significantly compared to preoperative values (p < 0.001). CSVL–C7PL, SVA, and TK were stable after surgery (p > 0.05) but considerably improved during follow‐up (p < 0.001). PI showed no significant change at either the postoperative or follow‐up timepoints.Lateral lumbar intervertebral fusion treatment of DLS significantly improved sagittal and coronal balance of the lumbar spine, as well as compensatory thoracic scoliosis, with good clinical and radiological findings. Furthermore, there was less blood, less trauma, and quicker recovery from surgery. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effect of different bone cement injection methods during percutaneous vertebroplasty(PVP) on vertebral morphology and cement diffusion., Methods: The clinical data of 52 patients with single-segment osteoporotic vertebral compression fracture treated from January 2016 to December 2017 were retrospectively analyzed. The patients were divided into hydraumatic group (28 cases) and pusher group (24 cases) according to bone cement injection method during PVP. By comparing visual analogue scale(VAS), height of anterior vertebral body, compression ratio, kyphosis angle before and after operation and analyzing filling ratio of bone cement in the first 1/3, median line and back 1/3 of the vertebral body in lateral X-rays and the conditions of bone cement diffusion in AP X-rays were to evaluate the effect of different bone cement injection methods on vertebral morphology and cement diffusion., Results: Postoperative VAS was obviously improved in all patients and hydraumatic group was better than pusher group( P <0.05). Postoperative height of anterior vertebral body, compression ratio and kyphosis angle obviously restored in all patients while there was no significant difference between two groups( P >0.05). There was no significant difference in filling ratio of bone cement in the first 1/3 and median line of the vertebral body by lateral X-ray films between two groups( P >0.05), but in the back 1/3 of the vertebral body filling ratio of bone cement in hydraumatic group was better than in pusher group( P <0.05). The distribution of bone cement from AP X-ray films were more significant in hydraumatic group( P <0.05)., Conclusions: Hydraulic delivery vertebroplasty (HDVP) has better clinical efficacy and it can guarantee sufficient distribution of bone cement into the fractured vertebra and preferably restore the morphology of vertebral body, which is worthy of clinical application., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© 2019 by the China Journal of Orthopaedics and Traumatology Press.)

The present study was performed to investigate the effect of retinoic acid amide (RAA) on the expression of integrin α3β1, rate of cell proliferation and migration in p53-deficient glioma cell line, LN-308. The results revealed promotion of integrin α3 expression, reduction in proliferation and migration in RAA treated cells compared to the control LN-308 glioma cells. Promotion of RAA induced integrin α3β1 expression led to the enhancement in cyclin-dependent kinase nuclear localization and activation of Akt pathway. In addition, RAA treatment inhibited the expression of nuclear factor-κB, Bcl-2 and epidermal growth factor receptor (EGFR). These factors are responsible for promoting the rate of cell proliferation and survival in the carcinoma cells. Thus RAA treatment inhibits rate of LN-308 glioma cell proliferation and migration through increase in integrin α3β1 expression and activation of Akt pathway. Therefore, RAA can be of therapeutic importance for the treatment of glioma.

Objective: To study the source of infection, the scope of epidemic and control measures in an outbreak involving students having symptoms as fever, dizziness, headache, vomiting and nausea., Methods: The suspected-case was defined as fever (armpit temperature ≥ 37°C) and with one or more of the following symptoms: dizziness, headache, vomiting and nausea, among students and teachers at school from Mar 1, 2012. Confirmed-case was among suspected case accompanied by both throat and rectal swabs enterovirus positive by RT-PCR. All the cases were collected through checking the medical records from 4 hospitals as well as through the absence records of students and teachers, from Mar 1, 2012. We conducted a case-control study with ratio of 1:2 and data on the exposures to water among students and teachers was collected prior to the illness. 27 cases' throat and rectal swabs were collected and analyzed by RT-PCR and PCR sequence methods. 2 warm-water samples were collected for testing the counts on total bacteria and E. coli., Results: 103 students' cases were identified in school L, with the attack rate as 4.6% (103/2255). Students from Grade three had the high attack rate as 18.1% (72/397) and 77.7% (80/103) of the cases located in the building with 'multiple-functions'. Epidemic curve of the outbreak showed a pattern with continuous common source of infection. It seemed that the exposure to warm-water appeared to be the major risk factor (OR = 18.3, 95%CI: 2.0 - 169.5) together with the intake of un-boiled water (OR = 15.5, 95%CI: 1.7 - 141.8). Specimens from 27 students (81.5%, 22/27) were identified enterovirus positive by RT-PCR, and 7 of the 9 students were confirmed carrying Echo 30. Bacteria and coli were negative from the 2 warm-water samples., Conclusion: This viral meningitis-outbreak was caused by Echo 30, with drinking water as the major risk factor.