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129 results on '"Lu, Wei-dong"'

46. Study on Preparation of Chitosan Coated Influenza Vaccine Liposome.

Author

QIAO Jian-bin, CHEN Na, LI qiang, LI Pei-hui, YAN He-gui, and LU Wei-dong

Abstract

Objective To prepare chitosan microparticles based on influenza vaccine liposome, and determine the preparation technology of chitosan liposome vaccine. Methods We prepared chitosan microparticles with different N/P ratio based on influenza vaccine liposomes which were produced by film dispersion method. Then we observed the morphology and determined the average particle size and particle size distribution, entrapment efficiency and the binding rate. By comparing the encapsulation rate and binding rate of different N/P ratio chitosan nanoparticles, the preparation technology of chitosan liposome vaccine was preliminary determined. Results Influenza vaccine liposomes coated with chitosan were round or oval with uniformed particle size distribution. The average particle size increased from 2.14 μ m to 4.05 μ m, and the average encapsulation rate increased from 80.41% to 84.37% after coated liposomes with chitosan . By comparing 6 N/P ratio, the ration of 5:1 was the best ratio in terms of binding rate which was 19.40% . Conclusions Chitosan coated liposomes is circular with increased particle size and encapsulation rate. 5:1 ratio of N/P is the optimum proportion concerning the incubation of chitosan and liposome. [ABSTRACT FROM AUTHOR]

Published
2014

47. Analysis of Protein Expression Profiles of Halobacillus dabanensis D-8T Under Optimal and High Salinity Conditions.

Author

Feng, De Qin, Yang, Li Fu, Lu, Wei Dong, and Yang, Su Sheng

Subjects
GEL electrophoresis, BACTERIAL proteins, SALINITY, MATRIX-assisted laser desorption-ionization, TIME-of-flight mass spectrometry
Abstract

Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8T under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemaster™ 2D Platinum software. The molecular masses of the majority of intracellular proteins were distributed in the range of 17.5 kDa–66 kDa and isoelectric points of 4.0–5.9. In total 133 protein spots were observed with a changed expression level under different salinity conditions. Sixty-two protein spots showed upregulation and 26 new protein spots were found under high salinity conditions, while 25 protein spots were downregulated and 20 spots disappeared. Twenty-seven proteins with a markedly changed expression in hypersaline environments were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF/MS) and MASCOT. A changed expression pattern was observed for proteins related to energy-producing pathways, stress regulators, and proteins involved in the survival of strain D-8T under high salt challenges. Many proteins play necessary roles in the adaptation to high salt or as a general stress protein, and some proteins are salt-stressed specific proteins that improve the capability of salt-tolerance of strain D-8T growth under extremely hypersaline condition. [ABSTRACT FROM AUTHOR]

Published
2007
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49. Two New Compounds from Saussurea graminea.

Author

Hu, Jiang, Shi, Xiaodong, Chen, Jiangang, Lu, Wei Dong, and Mao, Xia

Subjects
SAUSSUREA, PENTHRANE, ACETYL group, ETHANOL, HYDROXYCINNAMIC acids
Abstract

Two new compounds, 5,7-dihydroxy-4′-methoxyflavanone(3′→6)-5,7-dihydroxy-4′-methoxyflavone ( 1) and 1- O-[6- O-acetyl-β- D-glucopyranosyl]-3-hydroxycinnamic acid ( 2), were isolated from the 70% EtOH extract of Saussurea graminea. Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) analyses. [ABSTRACT FROM AUTHOR]

Published
2013
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50. Two New Compounds from Saussurea graminea.

Author

Hu, Jiang, Shi, Xiaodong, Chen, Jiangang, Lu, Wei Dong, and Mao, Xia

Subjects
*SAUSSUREA, *PENTHRANE, *ACETYL group, *ETHANOL, *HYDROXYCINNAMIC acids
Abstract

Two new compounds, 5,7-dihydroxy-4′-methoxyflavanone(3′→6)-5,7-dihydroxy-4′-methoxyflavone ( 1) and 1- O-[6- O-acetyl-β- D-glucopyranosyl]-3-hydroxycinnamic acid ( 2), were isolated from the 70% EtOH extract of Saussurea graminea. Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) analyses. [ABSTRACT FROM AUTHOR]

Published
2013
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