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Objective The study aimed to explore the mechanism of miR-133b regulating the invasion and migration of gastric cancer (GC) cells via the COL1A1/TGF-β axis. Methods The miRNA expression profiles of GC downloaded from TCGA database were subjected to differential analysis to determine the target miRNA of interest, and the target genes of the miRNA were predicted by bioinformatics. GSEA was used for gene enrichment analysis. qRT-PCR was carried out to detect gene expression in GC cells. The effect of miR-133b on GC cells was examined by CCK-8, wound healing and Transwell assays. Western blot was conducted to assess the protein expression of EMT-related proteins. The binding relationship between genes was verified by dual-luciferase reporter gene assay. Results The expression of miR-133b was markedly downregulated in GC tissue, while that of COL1A1 was upregulated. Overexpression of miR-133b decreased the migration and invasion of GC cells, and the EMT process was inhibited as well, while inverse results were observed when miR-133b was silenced. COL1A1 was a target gene of miR-133b and its overexpression had a significant impact on the prognosis of patients. GSEA pathway enrichment results showed that COL1A1 was markedly enriched in the TGF-β signaling pathway. In addition, COL1A1 overexpression induced the activation of the TGF-β signaling pathway to promote proliferation and migration of GC cells, whereas miR-133b overexpression suppressed the signaling pathway. Thus, overexpression of miR-133b and COL1A1 simultaneously would reverse the inhibitory effect of miR-133b on cell invasion and migration. Conclusion In this study, miR-133b was found to inhibit the invasion and migration of GC cells via the COL1A1/TGF-β axis, which provides a new research direction for the diagnosis and targeted therapy of GC.

According to the research of vertical migration and occurrence of Cd and As in acid paddy soil, the conclusions are as follows. The longitudinal migration of Cd in TZkb1, TZ1, TZ2, TZ3 and TZ5 found that Cd in the upper and lower layers of soil columns all lost to varying degrees with the increase of water injection contents (irrigation years). With the longitudinal leaching of irrigation water, when the adsorption capacity of the soil was greater than the water injection and rinsing capacity, the trend of accumulation (TZ4-TZ6) further appeared. The vertical migration results of As in TZkb1, TZ1, TZ2, TZ3 and TZ5 found that As mainly accumulated in the upper layer of soil columns. Without considering other output ways, it can be seen that the binding ability of As to acid paddy soil was stronger than Cd, and its cumulative efficiency in 20 years was higher than Cd. However Cd cumulative efficiency of TZ3 and TZ5 in acid paddy soil may be greater than As with time. In general, As was less affected by irrigation water on vertical leaching of soil columns compared with Cd.