Search

Your search keyword '"Lozic, Mirela"' showing total 8 results
Start Over Author "Lozic, Mirela"
8 results on '"Lozic, Mirela"'

5. Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development

Author

Racetin, Anita, Raguž, Fila, Durdov, Merica Glavina, Kunac, Nenad, Saraga, Marijan, Sanna-Cherchi, Simone, Šoljić, Violeta, Martinović, Vlatka, Petričević, Joško, Kostić, Sandra, Mardešić, Snježana, Tomaš, Sandra Zekić, Kablar, Boris, Restović, Ivana, Lozić, Mirela, Filipović, Natalija, Saraga-Babić, Mirna, and Vukojević, Katarina

Published
2019
Full Text
View/download PDF

7. CRKL , AIFM3 , AIF , BCL2 , and UBASH3A during Human Kidney Development.

Author

Lozic, Mirela, Minarik, Luka, Racetin, Anita, Filipovic, Natalija, Saraga Babic, Mirna, and Vukojevic, Katarina

Subjects
*KIDNEY development, *CELL survival, *NEPHRONS, *FETAL tissues, *KIDNEY tubules, *PROXIMAL kidney tubules
Abstract

We aimed to investigate the spatio-temporal expression of possible CAKUT candidate genes CRKL, AIFM3, and UBASH3A, as well as AIF and BCL2 during human kidney development. Human fetal kidney tissue was stained with antibodies and analyzed by fluorescence microscopy and RT-PCR. Quantification of positive cells was assessed by calculation of area percentage and counting cells in nephron structures. Results showed statistically significant differences in the temporal expression patterns of the examined markers, depending on the investigated developmental stage. Limited but strong expression of CRKL was seen in developing kidneys, with increasing expression up to the period where the majority of nephrons are formed. Results also lead us to conclude that AIFM3 and AIF are important for promoting cell survival, but only AIFM3 is considered a CAKUT candidate gene due to the lack of AIF in nephron developmental structures. Our findings imply great importance of AIFM3 in energy production in nephrogenesis and tubular maturation. UBASH3A raw scores showed greater immunoreactivity in developing structures than mature ones which would point to a meaningful role in nephrogenesis. The fact that mRNA and proteins of CRKL, UBASH3A, and AIFM3 were detected in all phases of kidney development implies their role as renal development control genes. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results