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Context: Habitat selection is a fundamental process that shapes animal spatial ecology. Species with wide geographic distribution that occupy diverse habitats have to adapt their resource acquisition strategies to maximise their effectiveness under local ecological constraints, leading to intraspecific behavioural variability. Identifying environmental determinants of habitat use pattern and regional intraspecific differences advances our understanding of the ecological underpinnings of animal behaviour and is important in strategising effective conservation and management of free-ranging populations. Aims: The aim of this study was to assess individual heterogeneity of habitat selection and use by African elephants under different seasonal and ecological constraints, in order to better understand the processes underlying their spatial behaviour. Methods: We investigated the habitat selection pattern of 19 African elephants equipped with satellite-linked GPS-collars in two different ecosystems, resource-rich bushveld bordering Kruger National Park, South Africa (six individuals) and arid savannah of Etosha National Park, Namibia (13 individuals). By constructing individual-specific and population-level resource selection functions (RSFs), we examined seasonal differences of elephant habitat use pattern to identify the underlying ecological mechanisms. Key results: Elephants were attracted to surface water in both study areas; but when water availability decreased in arid environment, they showed individual-specific preference in using natural vs artificial water sources. Road networks enabled efficient travel among resource patches, but its use differed between individuals. Areas with higher and more predictable vegetation productivity were generally preferred by elephants in dry season, but in more competitive arid savannah system there were individual/group-specific seasonal differences in resource selection patterns, likely reflecting the social dynamics among individuals. At population-level, the habitat selection pattern was less apparent due to considerable intra-population variability. Conclusions: The substantial differences in model coefficients within and between our study populations demonstrate the spatio-behavioural plasticity of elephants under various environmental conditions and suggest that population-level RSFs may over-simplify elephants' socio-ecological complexity. Implications: Within the resource competition paradigm, individual-specific habitat selection may be as essential in maintaining population resilience as is the population-level pattern of resource use. Consequently, spatio-behavioural heterogeneity within and between populations should be accounted for in resource selection studies and all subsequent conservation management policies. Habitat use pattern of animals is shaped by their biological needs and ecological dynamics of their environment. We examined habitat selection of African elephants in two different ecosystems in southern Africa, and found that individual responses to seasonally fluctuating resources varied considerably within and between populations. We conclude that individual-specific habitat selection pattern is as essential in maintaining population resilience as is the population-level pattern, and the intra- and inter-population spatio-behavioural heterogeneity have to be accounted for in conservation management. Photograph by Scott Y. S. Chui. [ABSTRACT FROM AUTHOR]

African savanna elephants are a highly mobile species that ranges widely across the diversity of ecosystems they inhabit. In xeric environments, elephant movement patterns are largely dictated by the availability of water and suitable forage resources, which can drive strong seasonal changes in their movement behavior. In this study, we analyzed a unique movement dataset from 43 collared elephants, collected over a period of 10 years, to assess the degree to which seasonal changes influences home range size of elephants in the semi‐arid, Laikipia‐Samburu ecosystem of northern Kenya. Auto‐correlated Kernel Density Estimation (AKDE) was used to estimate elephants' seasonal home range size. For each individual elephant, we also calculated seasonal home range shifts, as the distance between wet season home range centroids and dry season home range centroids. Core areas (50% AKDE isopleths) of all individual elephants ranged from 3 to 1743 km2 whereas total home range sizes (the 95% AKDE isopleths) ranged between 15 and 10,677 km2. Core areas and home range sizes were 67% and 61% larger, respectively, during the wet season than during the dry season. On average, the core area centroids for all elephants were 17 km away from the nearest river (range 0.2–150.3 km). Females had their core areas closer to the river than males (13.5 vs. 27.5 km). Females differed from males in their response to seasonal variation. Specifically, females tended to occupy areas farther from the river during the wet season, while males occupied areas further from the river during the dry season. Our study highlights how elephants adjust their space use seasonally, which can be incorporated into conservation area planning in the face of increased uncertainty in rainfall patterns due to climate change. [ABSTRACT FROM AUTHOR]

The African savanna elephant (Loxodonta africana) is the largest terrestrial animal on Earth and is found primarily in Southern and Eastern Africa. It is a hindgut, colonic fermenter and subsists on a diet of raw plant materials found in its grazing area. In this study the bacterial, archaeal and fungal populations of seven African savanna elephant fecal metagenomes were first characterized using amplicon sequencing. On the genus level it was observed that the p‐1088‐a5 gut group in the bacteriome, Methanocorpusulum and Methanobrevibacter in the archaeome and Alternaria, Aurobasidium, Didymella and Preussia in the mycome, predominated. Subsequently, metagenomic shotgun sequencing was employed to identify possible functional pathways and carbohydrate‐active enzymes (CAZymes). Carbohydrate catabolic pathways represented the main degradation pathways, and the fecal metagenome was enriched in the glycohydroside (GH) class of CAZymes. Additionally, the top GH families identified – GH43, GH2, GH13 and GH3 – are known to be associated with cellulytic, hemicellulytic and pectolytic activities. Finally, the CAZymes families identified in the African savanna elephant were compared with those found in the Asian elephant and it was demonstrated that there is a unique repository of CAZymes that could be leveraged in the biotechnological context such as the degradation of lignocellulose for the production of second‐generation biofuels and energy. [ABSTRACT FROM AUTHOR]

Context: Human modification of landscapes poses a significant threat to wildlife, particularly in Africa where population growth and land conversion are expected to increase. Habitat loss and fragmentation have led to declines in wildlife populations, highlighting the need to identify and preserve critical habitats, including core use areas and connectivity between them. Most recently, the identification of habitat corridors has become a key objective. Objectives: Our study objectives are to (1) empirically quantify connectivity across the Kunene—Etosha landscape in Northwestern Namibia using GPS tracking data on wild African elephants, and (2) assess the landscape features (i.e., geologic, biotic, and human-made) influencing connectivity and corridor types (e.g., fast movement corridors versus slow multi-use movement corridors). Methods: We used GPS telemetry data from 66 elephants collared in Northwestern Namibia to empirically quantify connectivity using a graph theoretic approach and assess landscape features influencing connectivity. Based on the ‘movescape’ approach, we identify different types of corridors and examined how landscape features differed across these corridors using multiple regression models on locations classified into different types of use categories by machine learning algorithms. Results: Our results revealed strong variation in connectivity across the landscape, with paths of high connectivity near water sources between the study areas. We found that factors related to water sources and human presence primarily influenced connectivity. Water holes serve as hubs across the ecosystem for both male and female elephants with lower use areas peripheral to areas with water. Connectivity between Kunene and Etosha National Park was relatively rare among the collared elephants, but we highlight the key areas used to move between the two regions. Conclusion: Water was the key feature structuring space use, and human presence influenced connectivity between water points, highlighting the importance of landscape planning in relation to limited water sources and human activities. Our results suggest that focusing management efforts on areas where water is limited for both elephants and humans will be important to reduce conflict and maintain ecosystem connectivity. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To evaluate the pharmacokinetics of famciclovir and its metabolite penciclovir following a single dose administered orally and rectally in African elephants (Loxodonta africana). ANIMALS: 15 African elephants (6 males and 9 females) of various ages. METHODS: Famciclovir (15 mg/kg) was administered orally or per rectum once, with at least a three-week washout period between administrations. Blood was collected at 13 different timepoints per administration for 6 elephants, occurring between February and March 2020. An additional 9 elephants were sampled at variable timepoints per administration utilizing a sparse sampling design between July 2020 and January 2021. Plasma famciclovir and penciclovir levels were measured via HPLC and fluorescence detection. Pharmacokinetic analysis was completed in the summer of 2021 using noncompartmental analysis and nonlinear mixed-effects modeling. RESULTS: Famciclovir was not detected in any sample, suggesting complete metabolism. Key pharmacokinetic parameters for penciclovir following oral administration were time to maximum concentration (tmax; 2.12 hours), area under the concentration-versus-time curve (AUC; 33.93 μg · h/mL), maximum observed concentration (Cmax; 3.73 μg/mL), and absorption half-life (t1/2; 0.65 hours). Following rectal administration, the values were: tmax, 0.65 hours; AUC, 15.62 μg·h/mL; Cmax, 2.52 μg/mL; and absorption t1/2, 0.13 hours. CONCLUSIONS: Famciclovir was rapidly metabolized to penciclovir. Oral administration resulted in slower absorption but higher maximum plasma concentration and higher AUC compared to rectal administration. CLINICAL RELEVANCE: African elephants administered famciclovir via oral and rectal routes resulted in measurable serum penciclovir, and these findings may be utilized by clinicians treating viral infections in this species. [ABSTRACT FROM AUTHOR]

Group-living animals engage in coordinated vocalizations to depart from a location as a group, and often, to come to a consensus about the direction of movement. Here, we document for the first time, the use of coordinated vocalizations, the "let's go" rumble, in wild male African elephant group departures from a waterhole. We recorded vocalizations and collected behavioral data as known individuals engaged in these vocal bouts during June-July field seasons in 2005, 2007, 2011, and 2017 at Mushara waterhole within Etosha National Park, Namibia. During departure events, we documented which individuals were involved in the calls, the signature structure of each individual's calls, as well as the ordering of callers, the social status of the callers, and those who initiated departure. The "let's go" rumble was previously described in tight-knit family groups to keep the family together during coordinated departures. Male elephants are described as living in loose social groups, making this finding particularly striking. We found that this vocal coordination occurs in groups of closely associated, highly bonded individuals and rarely occurs between looser associates. The three individuals most likely to initiate the "let's go" rumble bouts were all highly socially integrated, and one of these individuals was also the most dominant overall. This finding suggests that more socially integrated individuals might be more likely to initiate, or lead, a close group of associates in the context of leaving the waterhole, just as a high-ranking female would do in a family group. The fact that many individuals were involved in the vocal bouts, and that departure periods could be shorter, longer, or the same amount of time as pre-departure periods, all suggest that there is consensus with regard to the act of leaving, even though the event was triggered by a lead individual. [ABSTRACT FROM AUTHOR]

Climate change, land use conversion and human population growth are reducing and fragmenting historical ranges of large animals, especially in Africa. Particularly, land conversion to agriculture is leading to coexistence challenges between humans and elephants. In this study we investigated the factors affecting the intensity of elephant crop damage in the Selous-Niassa Wildlife Corridor (Tanzania). We also predicted future conflicts (crop damage intensity) for different land management (crop types) and water scarcity (drought) scenarios using model averaging. Our results show that intensity of elephant damage (proportion of area damaged in an individual farm as a percentage) increased with crop palatability and land use conversion in the last ∼30 years, particularly deforestation. Conversely, the intensity of elephant damage decreased with increasing human activity (higher proportion of settlements and farmland), and distance to waterbodies. Most farms affected by elephants (65 %) were at short distances (< 250 m) from waterbodies. We also predicted a significant increase of elephant crop damage intensity from 17 % when the farm is covered with no palatable crops, to 54 % and 63 % when palatable crops covered 50 % and 100 % of the farmland, respectively. For the predicted water stress scenario in which all small seasonal waterbodies would dry off, we predicted a 46 % reduction in the total area of farmland susceptible to damage although we expect an increase in human-wildlife competition for water. We conclude that land use changes and water availability strongly affect elephant crop damage. We hope these results contribute to the development and better implementation of management strategies that enhance long-term peaceful coexistence between humans and elephants.

Abstract Conflict between humans and elephants is one of the more complex examples of human‐wildlife conflict, a key challenge for wildlife conservation. While interventions exist to separate humans from elephants, few exist aimed at bringing the two species closer together. This study assesses if a natural history film, The Elephant Queen (TEQ), makes communities living around elephants more tolerant of conflict with elephants. Questionnaires and interviews were conducted before and after seeing the film screened on a mobile cinema in Southern Kenya. A double robust ordinal regression analysis using 357 matching specifications to measure the effect size of viewing TEQ on the six criteria identified as being drivers of tolerance of a wild animal by the Hazard Acceptance model. This study found that students aged between 16 and 18 gained knowledge (mean effect size = 0.27) and affection (mean effect size = 0.17) towards elephants and felt the benefits of elephants more keenly (mean effect size = 0.26) following viewing TEQ. Community members aged between 16–80 also gained knowledge (mean effect size = 0.21) and saw the benefits of elephants (mean effect size = 0.15) but felt the costs of living with elephants more profoundly after viewing TEQ (mean effect size = −0.11). After 90 days a follow‐up survey also showed a significant increase in community “affection” towards elephants (mean effect size = 0.11), however the costs, benefits and knowledge gained had been reduced to a statistically insignificant level compared to baseline. Our results suggest that natural history films can serve as a valuable tool in inspiring young minds. When shown to an adult audience, changes were more nuanced and some of the changes were short lived (

Among tetrapods, grasping is an essential function involved in many vital behaviours. The selective pressures that led to this function were widely investigated in species with prehensile hands and feet. Previous studies namely highlighted a strong effect of item properties but also of the species habitat on manual grasping behaviour. African savannah elephants (Loxodonta africana) are known to display various prehensile abilities and use their trunk in a large diversity of habitats. Composed of muscles and without a rigid structure, the trunk is a muscular hydrostat with great freedom of movement. This multitasking organ is particularly recruited for grasping food items while foraging. Yet, the diet of African savannah elephants varies widely between groups living in different habitats. Moreover, they have tusks alongside the trunk which can assist in grasping behaviours, and their tusk morphologies are known to vary considerably between groups. Therefore, in this study, we investigate the food grasping techniques used by the trunk of two elephant groups that live in different habitats: an arid study site in Etosha National Park in Namibia, and an area with consistent water presence in Kruger National Park in South Africa. We characterised the tusks profiles and compared the grasping techniques and their frequencies of use for different foods. Our results show differences in food‐grasping techniques between the two groups. These differences are related to the food item property and tusk profile discrepancies highlighted between the two groups. We suggest that habitat heterogeneity, particularly aridity gaps, may induce these differences. This may reveal an optimisation of grasping types depending on habitat, food size and accessibility, as well as tusk profiles. [ABSTRACT FROM AUTHOR]

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Abstract African savanna elephants are a highly mobile species that ranges widely across the diversity of ecosystems they inhabit. In xeric environments, elephant movement patterns are largely dictated by the availability of water and suitable forage resources, which can drive strong seasonal changes in their movement behavior. In this study, we analyzed a unique movement dataset from 43 collared elephants, collected over a period of 10 years, to assess the degree to which seasonal changes influences home range size of elephants in the semi‐arid, Laikipia‐Samburu ecosystem of northern Kenya. Auto‐correlated Kernel Density Estimation (AKDE) was used to estimate elephants' seasonal home range size. For each individual elephant, we also calculated seasonal home range shifts, as the distance between wet season home range centroids and dry season home range centroids. Core areas (50% AKDE isopleths) of all individual elephants ranged from 3 to 1743 km2 whereas total home range sizes (the 95% AKDE isopleths) ranged between 15 and 10,677 km2. Core areas and home range sizes were 67% and 61% larger, respectively, during the wet season than during the dry season. On average, the core area centroids for all elephants were 17 km away from the nearest river (range 0.2–150.3 km). Females had their core areas closer to the river than males (13.5 vs. 27.5 km). Females differed from males in their response to seasonal variation. Specifically, females tended to occupy areas farther from the river during the wet season, while males occupied areas further from the river during the dry season. Our study highlights how elephants adjust their space use seasonally, which can be incorporated into conservation area planning in the face of increased uncertainty in rainfall patterns due to climate change.

Abstract Understanding drivers of space use by African elephants is critical to their conservation and management, particularly given their large home-ranges, extensive resource requirements, ecological role as ecosystem engineers, involvement in human-elephant conflict and as a target species for ivory poaching. In this study we investigated resource selection by elephants inhabiting the Greater Mara Ecosystem in Southwestern Kenya in relation to three distinct but spatially contiguous management zones: (i) the government protected Maasai Mara National Reserve (ii) community-owned wildlife conservancies, and (iii) elephant range outside any formal wildlife protected area. We combined GPS tracking data from 49 elephants with spatial covariate information to compare elephant selection across these management zones using a hierarchical Bayesian framework, providing insight regarding how human activities structure elephant spatial behavior. We also contrasted differences in selection by zone across several data strata: sex, season and time-of-day. Our results showed that the strongest selection by elephants was for closed-canopy forest and the strongest avoidance was for open-cover, but that selection behavior varied significantly by management zone and selection for cover was accentuated in human-dominated areas. When contrasting selection parameters according to strata, variability in selection parameter values reduced along a protection gradient whereby elephants tended to behave more similarly (limited plasticity) in the human dominated, unprotected zone and more variably (greater plasticity) in the protected reserve. However, avoidance of slope was consistent across all zones. Differences in selection behavior was greatest between sexes, followed by time-of-day, then management zone and finally season (where seasonal selection showed the least differentiation of the contrasts assessed). By contrasting selection coefficients across strata, our analysis quantifies behavioural switching related to human presence and impact displayed by a cognitively advanced megaherbivore. Our study broadens the knowledge base about the movement ecology of African elephants and builds our capacity for both management and conservation.

Beehive deterrents are commonly used to mitigate human–elephant conflict and protect woody vegetation. To ensure hive activity, reduce abscondment risks, and maintain deterrent effectiveness, resident bee colonies require supplementary feeding during periods of low resource availability. However, our study found that ants frequently consume the supplementary feed in open feeders intended for bees. Anoplolepis custodiens was the most numerically dominant species that excluded bees from the feeders, followed by Camponotus and Crematogaster spp. With higher ant abundance, the predicted probability of zero bees being present at feeders increased up to 82%. This competition may undermine the efficacy of beehive deterrents as a conflict mitigation tool. We developed a simple and effective ant exclusion method that raised the overall predicted probability of bees' presence at supplementary feeding stations from 32% to 68%. Our findings suggest that innovative solutions to exclude ants from supplementary feed may improve the implementation and success of this conflict mitigation method across Africa. [ABSTRACT FROM AUTHOR]

Abstract Among tetrapods, grasping is an essential function involved in many vital behaviours. The selective pressures that led to this function were widely investigated in species with prehensile hands and feet. Previous studies namely highlighted a strong effect of item properties but also of the species habitat on manual grasping behaviour. African savannah elephants (Loxodonta africana) are known to display various prehensile abilities and use their trunk in a large diversity of habitats. Composed of muscles and without a rigid structure, the trunk is a muscular hydrostat with great freedom of movement. This multitasking organ is particularly recruited for grasping food items while foraging. Yet, the diet of African savannah elephants varies widely between groups living in different habitats. Moreover, they have tusks alongside the trunk which can assist in grasping behaviours, and their tusk morphologies are known to vary considerably between groups. Therefore, in this study, we investigate the food grasping techniques used by the trunk of two elephant groups that live in different habitats: an arid study site in Etosha National Park in Namibia, and an area with consistent water presence in Kruger National Park in South Africa. We characterised the tusks profiles and compared the grasping techniques and their frequencies of use for different foods. Our results show differences in food‐grasping techniques between the two groups. These differences are related to the food item property and tusk profile discrepancies highlighted between the two groups. We suggest that habitat heterogeneity, particularly aridity gaps, may induce these differences. This may reveal an optimisation of grasping types depending on habitat, food size and accessibility, as well as tusk profiles.

This study evaluated the proximate and mineral composition of various parts of Balanites aegyptiaca, Mitragyna inermis, Cassia sieberiena, Kigelia africana, Detarium microcarpum, Terminalia laxiflora, Ficus platyphylla and Climber plant (Liana) as feeds of elephants in the savannah ecological zone of Ghana. The proximate composition was carried out using the methods of the Association of official analytical chemists (AOAC) and the mineral profiles were determined by atomic absorption and flame photometric methods. The highest content of Crude Protein, Crude Fibre, Ash, Moisture and Nitrogen free extract were 12.0% ± 0.01, 15.3% ± 0.01, 14.6% ± 0.0, 7.5% ± 0.01, 76.6% ± 0.01 for Ficus platyphylla, Tamarindus indica, Balanites aegyptiaca and Detarium microcarpum, respectively. The mineral profiling also found appreciable levels of Ca, Mg, P, K, Na, Zn, Cu, Fe, Se and Mn at concentrations (mg/kg) of 4.6 ± 0.00, 0.4 ± 0.01, 0.4 ± 0.01, 5.1 ± 0.01, 0.11 ± 0.01, 1.07 ± 0.008, 0.25 ± 0.005, 0.063 ± 0.0005, 37.48 ± 0.001 for Ficus platyphylla bark, Liana, Terminalia laxiflora bark and leaves, Mitragyna inermis bark, Terminalia laxiflora leaves and Mitragyna inermis leaves, respectively. Presentation of the presence of these important nutrients would be a guide to zoo managers and wildlife practitioners on the best quality of feeds to select for wild elephants.

This study evaluated the proximate and mineral composition of various parts of Balanites aegyptiaca, Mitragyna inermis, Cassia sieberiena, Kigelia africana, Detarium microcarpum, Terminalia laxiflora, Ficus platyphylla and Climber plant (Liana) as feeds of elephants in the savannah ecological zone of Ghana. The proximate composition was carried out using the methods of the Association of official analytical chemists (AOAC) and the mineral profiles were determined by atomic absorption and flame photometric methods. The highest content of Crude Protein, Crude Fibre, Ash, Moisture and Nitrogen free extract were 12.0% ± 0.01, 15.3% ± 0.01, 14.6% ± 0.0, 7.5% ± 0.01, 76.6% ± 0.01 for Ficus platyphylla, Tamarindus indica, Balanites aegyptiaca and Detarium microcarpum, respectively. The mineral profiling also found appreciable levels of Ca, Mg, P, K, Na, Zn, Cu, Fe, Se and Mn at concentrations (mg/kg) of 4.6 ± 0.00, 0.4 ± 0.01, 0.4 ± 0.01, 5.1 ± 0.01, 0.11 ± 0.01, 1.07 ± 0.008, 0.25 ± 0.005, 0.063 ± 0.0005, 37.48 ± 0.001 for Ficus platyphylla bark, Liana, Terminalia laxiflora bark and leaves, Mitragyna inermis bark, Terminalia laxiflora leaves and Mitragyna inermis leaves, respectively. Presentation of the presence of these important nutrients would be a guide to zoo managers and wildlife practitioners on the best quality of feeds to select for wild elephants. [ABSTRACT FROM AUTHOR]

The gut microbiota plays a crucial role in animal health and homeostasis, particularly in endangered species conservation. This study investigated the fecal microbiota composition of European captive-bred African savanna elephants (Loxodonta africana) housed in French zoos, and compared it with wild African savanna elephants. Fecal samples were collected and processed for DNA extraction and amplicon sequencing of the 16S rRNA gene. The analysis of α and ß diversity revealed significant effects of factors such as diet, daily activity, and institution on microbiota composition. Specifically, provision of branches as part of the diet positively impacted microbiota diversity. Comparative analyses demonstrated distinct differences between captive and wild elephant microbiomes, characterized by lower bacterial diversity and altered co-occurrence patterns in the captive population. Notably, specific taxa were differentially abundant in captive and wild elephants, suggesting the influence of the environment on microbiota composition. Furthermore, the study identified a core association network shared by both captive and wild elephants, emphasizing the importance of certain taxa in maintaining microbial interactions. These findings underscore the impact of environment and husbandry factors on elephant gut microbiota, highlighting the benefits of dietary enrichment strategies in zoos to promote microbiome diversity and health. The study contributes to the broader understanding of host-microbiota interactions and provides insights applicable to conservation medicine and captive animal management. [ABSTRACT FROM AUTHOR]

Simple Summary: In the wild, African elephant calves must stay close to their mothers and the family unit as the African environment holds many threats. African elephant calves in zoos are raised in a protected environment. Therefore, we hypothesize that calves ex situ hold bigger distances and behave differently than in situ. Additionally, those differences are likely to increase with further zoo generations. This study used ethological research methods to compare the mother–calf bond of African elephant calves in situ and ex situ (first and second generation). The results showed that ex situ living calves of both generations maintain greater distances to their mothers and show a wider variation (positive and negative) in behavior than in situ. The detected differences indicate that calves ex situ can behave more freely as they are in a protected environment. Therefore, they can develop faster than in the wild, which agrees with similar findings on African elephant calf development and adult African elephants. The hypothesis that differences between in situ and ex situ increase with the zoo generations could not be verified. Hence, modifications in behavior under different environmental selection pressures may be adaptive. African zoo elephants live in safe environments with sufficient resources, are protected from threats, and have their health and body conditions cared for. Calves ex situ undergo the same developmental stages as in situ and are raised by the whole family unit. However, due to environmental differences, there might be behavioral modifications between calves in situ and ex situ. We hypothesize that these differences increase with ongoing generations. This ethological study compares social and general behavior and the distance calves kept to their mothers' between calves of the first (F1) and second (F2) zoo generation and the wild. Using ethological methods, data were collected for ~90 in situ calves and 16 ex situ (8 F1, 8 F2) between the ages of 0.5 to 4 years (120 observation hours per group). Results showed that in situ calves spent significantly more time close to mothers than the F1 and the F2 zoo generations (F1/in situ: p = <0.001; F2/in situ: p = 0.007). The behaviors of eating, drinking, trunk movement, washing, and affiliative behaviors showed significant differences between in situ and ex situ calves. The amount and distribution of affiliative and agonistic behavior initiated and received by calves was displayed with a greater variety ex situ. Ex situ calves not only performed affiliative but, in contrast to the in situ, also agonistic behavior (F1/in situ: initiated p = 0.002, received p = 0.010; F2/in situ: initiated p = 0.050, received p = 0.037). The comparison of zoo generations suggests that differences did not increase with the generation. The more casual binding between mothers and offspring in zoos and the age-dependent improvement of social behavior of zoo-born calves are seen as a result of elephants' adaptation to secure zoo conditions. The results of this study agree with the faster development of ex situ African elephants, like earlier puberty and more frequent breeding patterns, as known from the literature. [ABSTRACT FROM AUTHOR]

When wide ranging, herbivorous species such as African elephants (Loxodonta africana) are confined and their population densities increase, the vegetation may be negatively affected. Any changes to vegetation structure and complexity may then result in a series of ecological cascades on other plant and animal species that rely on the vegetation for survival. We assessed the composition and structure of the woody and succulent plants within the locally endemic Albany Thicket Biome at five protected areas (reserves) with elephants in the Eastern Cape province, South Africa in 2006 and 2016. We show that when elephant populations in this region are actively managed, wholesale negative impacts on woody thicket plant physiognomy are not observed. In fact, the only statistically detectable change over time, presumably due to elephants, was the toppling of larger, single-stemmed trees. We believe that because the Albany Thicket Biome evolved in the presence of large, top-down browsers such as elephants, the generally negative effects that have been observed of elephants on vegetation in savanna systems are not evident. However, the reserves with elephants in the Eastern Cape are all relatively small (< 400 km2) and fenced which prevents elephant dispersal. Thus, continued monitoring of elephant browsing effects is crucial for the future conservation of this unique biome. [ABSTRACT FROM AUTHOR]

The gut microbiota plays a crucial role in animal health and homeostasis, particularly in endangered species conservation. This study investigated the fecal microbiota composition of European captive-bred African savanna elephants (Loxodonta africana) housed in French zoos, and compared it with wild African savanna elephants. Fecal samples were collected and processed for DNA extraction and amplicon sequencing of the 16S rRNA gene. The analysis of α and β diversity revealed significant effects of factors such as diet, daily activity, and institution on microbiota composition. Specifically, provision of branches as part of the diet positively impacted microbiota diversity. Comparative analyses demonstrated distinct differences between captive and wild elephant microbiomes, characterized by lower bacterial diversity and altered co-occurrence patterns in the captive population. Notably, specific taxa were differentially abundant in captive and wild elephants, suggesting the influence of the environment on microbiota composition. Furthermore, the study identified a core association network shared by both captive and wild elephants, emphasizing the importance of certain taxa in maintaining microbial interactions. These findings underscore the impact of environment and husbandry factors on elephant gut microbiota, highlighting the benefits of dietary enrichment strategies in zoos to promote microbiome diversity and health. The study contributes to the broader understanding of host-microbiota interactions and provides insights applicable to conservation medicine and captive animal management.

Background: The distribution of resources can affect animal range sizes, which in turn may alter infectious disease dynamics in heterogenous environments. The risk of pathogen exposure or the spatial extent of outbreaks may vary with host range size. This study examined the range sizes of herbivorous anthrax host species in two ecosystems and relationships between spatial movement behavior and patterns of disease outbreaks for a multi-host environmentally transmitted pathogen. Methods: We examined range sizes for seven host species and the spatial extent of anthrax outbreaks in Etosha National Park, Namibia and Kruger National Park, South Africa, where the main host species and outbreak sizes differ. We evaluated host range sizes using the local convex hull method at different temporal scales, within-individual temporal range overlap, and relationships between ranging behavior and species contributions to anthrax cases in each park. We estimated the spatial extent of annual anthrax mortalities and evaluated whether the extent was correlated with case numbers of a given host species. Results: Range size differences among species were not linearly related to anthrax case numbers. In Kruger the main host species had small range sizes and high range overlap, which may heighten exposure when outbreaks occur within their ranges. However, different patterns were observed in Etosha, where the main host species had large range sizes and relatively little overlap. The spatial extent of anthrax mortalities was similar between parks but less variable in Etosha than Kruger. In Kruger outbreaks varied from small local clusters to large areas and the spatial extent correlated with case numbers and species affected. Secondary host species contributed relatively few cases to outbreaks; however, for these species with large range sizes, case numbers positively correlated with outbreak extent. Conclusions: Our results provide new information on the spatiotemporal structuring of ranging movements of anthrax host species in two ecosystems. The results linking anthrax dynamics to host space use are correlative, yet suggest that, though partial and proximate, host range size and overlap may be contributing factors in outbreak characteristics for environmentally transmitted pathogens. [ABSTRACT FROM AUTHOR]

Simple Summary: It is paramount to preserve the genetic material of endangered wildlife species for future generations, including that of the African savanna elephant (Loxodonta africana). By analyzing their genetic information, it is possible to decipher how endangered species such as elephants adapted to live on our planet and how they are related to one another and to their extinct ancestors. This information can further assist us in the protection of endangered animals and the habitats they live in. One way of doing this is through the cryopreservation of elephants' primary cell lines. This protocol presents the successful establishment of primary dermal fibroblast cell lines using a simple explant outgrowth method, starting from a small punch biopsy sample, which is minimally invasive and allows sample collection of free-roaming elephants. The average explant outgrowth, doubling time and further characterization of the dermal fibroblast cells of different elephants have been evaluated and compared. This preliminary method illustrates the potential to establish cell lines from living elephants, which contributes to the conservation of their genetic material and allows further research on the cancer suppressing ability of elephants. Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material. [ABSTRACT FROM AUTHOR]

Elephant endotheliotropic herpesvirus (EEHV) hemorrhagic disease (EEHV-HD) threatens Asian elephant (Elephas maximus) population sustainability in North America. Clusters of cases have also been reported in African elephants (Loxodonta africana). Risk to range country elephant populations is unknown. Currently, EEHV detection depends upon sampling elephants trained for invasive blood and trunk wash collection. To evaluate noninvasive sample collection options, paired invasively collected (blood, trunk wash and oral swabs), and noninvasively collected (chewed plant and fecal) samples were compared over 6 wk from 9 Asian elephants and 12 African elephants. EEHV shedding was detected simultaneously in a paired trunk wash and fecal sample from one African elephant. Elephant γ herpesvirus-1 shedding was identified in six chewed plant samples collected from four Asian elephants. Noninvasively collected samples can be used to detect elephant herpesvirus shedding. Longer sampling periods are needed to evaluate the clinical usefulness of noninvasive sampling for EEHV detection.

While spontaneous yawning is common across all vertebrate classes, contagious yawning is less common and has been observed only in a few species of social animals. Interspecific contagious yawning in response to yawning by humans has been observed only by chimpanzees and dogs. After confirming additional occurrences of intraspecific contagious yawning in a group of captive African elephants previously studied, we further investigated the potential for the same group of elephants to engage in interspecific contagious yawning with familiar human handlers. Ten captive African elephants, most of whom had been previously studied, were observed over 13 nights for evidence of intraspecific contagious yawning. Seven of these elephants were also involved in trials where familiar handlers performed staged yawns, as well as trials with staged non-yawning gapes, or trials with no yawns or gapes. Incorporating previously collected contagious yawning data, we describe nine instances of intraspecific contagious yawning in the elephants. Three of the seven elephants yawned contagiously in response to humans during the interspecific yawning trials. This is the first report of interspecific contagious yawning by elephants in response to yawns by familiar humans.

Blood samples are routinely collected from wild populations in remote locations with limited electricity, minimal diagnostic capabilities, and extreme environmental conditions. Under these conditions, serum samples may be stored for prolonged time under varied temperatures prior to processing, which could affect the ability to interpretation the results. This study’s objective was to evaluate the effects of delayed processing of serum samples and varied storage temperatures on biochemical values in African savanna elephants (Loxodonta africana). Blood samples were collected from six elephants managed by the North Carolina Zoo. For each elephant, seven red top tubes were collected. One serum sample for each elephant was analyzed on Day 0 (control group). The remaining samples were stored under different temperatures including room temperature (23 °C), refrigeration (2.2 °C), and incubation (32.2 °C), with samples from each temperature group being analyzed on Day 5 and Day 10. Many of analytes (10 out of 18) did not change significantly regardless of storage temperature or time. Refrigeration improved stability in an additional four analytes over prolonged storage. We conclude that if serum is properly separated shortly after collection, many serum biochemical analytes can be accurately measured even after suboptimal serum storage, but refrigeration and prompt evaluation are still required for some analytes.

Abstract The African savannah ecosystem is populated by nomadic pastoralists who herd livestock in the day and corral them at night in temporary enclosures, called bomas, to protect them. The number and distribution of bomas on the savannah is important from an ecological perspective and may have a significant impact on wildlife movement. However, no study has yet examined this relationship. Here, using very high‐resolution satellite imagery from two time periods, we quanitified changes in boma distribution and density across an area of 3377 km2 in the Laikipia‐Samburu ecosystem of northern Kenya between 2011 and 2019. To assess wildlife movement in relation to bomas, we used a GPS data set on African bush elephant Loxodonta africana movement from 27 collared matriarchs representing herds of 9–15, covering 112 467 hourly GPS fixes over 31 months between 2018 and 2020. Our results showed a more than 46% increase in the total number of human‐built structures between 2011 and 2019, the majority of which were bomas, representing a 21.9% increase in human‐modified land area. Elephants readily adjusted their foraging habits and itineraries in this habitat shared with humans, who were also nomadic in space and time. Assessing the night–day activity ratio, we found elephants move more nocturnally when in closer proximity to bomas, particularly during the dry season. This temporal separation means elephants avoid the times humans are active in and around bomas while still accessing required resources—water and forage. The temporal shift was stronger during the dry season when shared resources are scarce. Using daily travel distance as a metric, we show elephants moved further in closer proximity to bomas which was likely linked to the need to travel between forage patches. Given the rise in human settlements, understanding the consequences of animals' behavioral adjustments is critical to understand the long‐term population viability of elephant populations.

This study compared hematocrit measured with the EPOC and i-STAT Alinity v point-of-care analyzers and manual measurement of packed cell volume in managed African savanna elephants (Loxodonta africana) and southern white rhinoceros (Ceratotherium simum simum). Biochemical analytes were also measured with the EPOC, i-STAT Alinity v, and a veterinary diagnostic laboratory in the same animals. Analytes assessed included blood urea nitrogen, chloride, creatinine, glucose, ionized calcium, potassium, and sodium. There were no differences for hematocrit values for African savanna elephants or southern white rhinoceros (p ≤ 0.05). In African savanna elephants, there were no differences between the EPOC and i-STAT Alinity v analyzers for any measured analytes except ionized calcium. When compared to a veterinary diagnostic laboratory, there were differences for a majority of the biochemical analytes measured on the EPOC and i-STAT Alinity v analyzers in African savanna elephants. In southern white rhinoceros, there were differences for a majority of analytes among all three analyzers. While differences existed among the portable analyzers and a veterinary diagnostic laboratory for biochemical analytes in both species, these numerically small differences are unlikely to be clinically significant. For routine health care of African savanna elephants and southern white rhinoceros, these point-of-care analyzers may be a useful alternative to commercial analyzers for the parameters evaluated.

Abstract Background Mycobacterium tuberculosis complex (MTC) that causes the chronic infectious disease- tuberculosis (TB), often presents with a complicated epidemiological pattern where the transmission chain may include humans, domestic animals and wildlife, including elephants. TB has been reported globally in both captive and wild elephants. The One Health approach might be the most effective way of understanding the shared MTC infection dynamics in captive and wild animals like Asian elephants. This systematic review accumulates evidence on occurrence, transmission pathways, and preventive measures of TB in elephants from a One Health perspective. Results The prevalence of TB reported in elephant populations ranges from 0 to 23.33% and high prevalence’s are reported for elephants that are in close proximity to infected humans. The risk of elephant to human infection transmission increased significantly with exposure duration and contact with infected elephants. Some studies described the plausible TB transmission to captive elephants from other animals (wild and domestic), suggesting inter- and intra-species transmission. The results of this systematic review based on 27 relevant published works, suggest three overarching interrelated transmission pathways for M. tuberculosis infections in Asian elephants- i) humans and elephants, ii) other animals (wild or domestic) and elephants and iii) unclear sources of infection. Conclusions The progress made with new TB diagnostic tools provides multiple methods to choose from. However, lack of harmonization of TB testing in elephants and their human contacts remains a challenge to prevent TB in those animals. Routine TB screening among elephants and caretakers by setting up an occupational health program for early diagnosis of infection through combined efforts of public health, veterinary medicine, and occupational health experts is suggested. This implies the need for a One Health approach to elephant TB control. This review reveals the need for more research on Mycobacterium tuberculosis complex transmission pathways at the human-animal interface.

Blood samples are routinely collected from wild populations in remote locations with limited electricity, minimal diagnostic capabilities, and extreme environmental conditions. Under these conditions, serum samples may be stored for prolonged time under varied temperatures prior to processing, which could affect the ability to interpretation the results. This study's objective was to evaluate the effects of delayed processing of serum samples and varied storage temperatures on biochemical values in African savanna elephants (Loxodonta africana). Blood samples were collected from six elephants managed by the North Carolina Zoo. For each elephant, seven red top tubes were collected. One serum sample for each elephant was analyzed on Day 0 (control group). The remaining samples were stored under different temperatures including room temperature (23 °C), refrigeration (2.2 °C), and incubation (32.2 °C), with samples from each temperature group being analyzed on Day 5 and Day 10. Many of analytes (10 out of 18) did not change significantly regardless of storage temperature or time. Refrigeration improved stability in an additional four analytes over prolonged storage. We conclude that if serum is properly separated shortly after collection, many serum biochemical analytes can be accurately measured even after suboptimal serum storage, but refrigeration and prompt evaluation are still required for some analytes. [ABSTRACT FROM AUTHOR]

African savanna elephants (Loxodonta africana) are a keystone species in African ecosystems. As a result of increasing anthropogenic pressure, elephant populations have declined significantly in the last two centuries. Research on a broad sample of these populations is necessary to inform management strategies over a range of environmental and socio-political conditions. In order to evaluate the current state of literature that is informing evidence-based management and conservation of elephants, we systematically reviewed all research published on the ecology of African elephants from the last 20 years (492 publications). We contrasted the geographic distribution of published research against the 2016 IUCN elephant census. We found several statistically significant biases in the geographic distribution of elephant research. South Africa has 4.54% of the total elephant population and accounted for 28.28% of all research publications. Kenya has 5.49% the total elephant population but accounts for 20.6% of the research. Conversely, Botswana has 31.68% of the total elephant population but accounts for only 9.29% of the research and Zimbabwe has 19.89% of the total elephant population with only 10.50% of research. We also found that 41.85% of areas with ~60,100 elephants have not had any research published on their populations in the last 20 years. This publication imbalance may encourage management strategies that are overly dependent on misrepresentative information from a small subset of the elephant population. We recommend that (1) marginalised areas with large elephant populations (e.g., Botswana and Zimbabwe) should receive higher priority for future research, (2) new research and proposals should design theoretical frameworks to account for and overcome the present biases, and (3) local community-based management approaches should be prioritised and amplified in order to overcome the barriers to conducting research in priority areas. [ABSTRACT FROM AUTHOR]

Elephants face diverse threats from human activities and use temporal and social strategies to reduce human‐induced mortality risk. We used data from camera trap surveys in 2018–2019 (n = 1625 independent detection events from 11,751 sampling days) to investigate elephant responses to anthropogenic risk in the Ruaha‐Rungwa ecosystem, Tanzania. The study was conducted in one low‐risk and three high‐risk sites using 26–40 paired camera trap stations per site. Risk influenced the active periods, use of roads and water sources, social associations and behaviour of elephants. Elephants demonstrated significantly more night‐time and reduced daytime activity in the high‐risk sites relative to the low‐risk site. This higher night‐time activity in the high‐risk sites was observed for both males and females, though it was more pronounced for cow–calf groups than lone males. Foraging events and use of water sources were more frequent at night in the high‐risk sites. Elephants used roads as movement routes in the low‐risk site but avoided roads in the high‐risk sites. Males were significantly more likely to associate with other males and cow–calf groups in the high‐risk sites. Fewer occurrences of relaxed behaviours were observed in the high‐risk sites compared to the low‐risk site. We discuss the potential implications of our findings for elephant survival and reproduction. [ABSTRACT FROM AUTHOR]

Simple Summary: African elephants are known for their long memory; this is also valid for their olfactory sense and their ability to discriminate scents. This feature is highly important for these mammals to maintain their family bonds and to differentiate between familiar and unfamiliar individuals. Thus far, scientific data only testify to an olfactory memory of up to one year for African elephants. This study investigated the long-term olfactory memory of two mother-daughter pairs that were separated for 2 and 12 years, respectively. Results showed that all four elephants were able to recognize their separated relatives just by the scent of feces, thereby giving the empirical implication of olfactory memory in African elephants of up to 12 years. African elephants are capable of discriminating scents up to a single changed molecule and show the largest reported repertoire of olfactory receptor genes. Olfaction plays an important role in family bonding. However, to the best of our knowledge, no empirical data exist on their ability to remember familiar scents long-term. In an ethological experiment, two mother-daughter pairs were presented with feces of absent kin, absent non-kin, and present non-kin. Video recordings showed reactions of elephants recognizing kin after long-term separation but only minor reactions to non-kin. Results give the empirical implication that elephants have an olfactory memory longer than 1 year and up to 12 years and can distinguish between kin and non-kin just by scent. These findings confirm the significance of scent for family bonds in African elephants. [ABSTRACT FROM AUTHOR]

Simple Summary: African elephants commonly enter farmlands across their ranges and trample or consume crops, creating conflicts with farmers and challenges for conservation priorities. Farmers may retaliate and injure elephants, but the extent to which this occurs is relatively unknown. However, scars found on elephants may be evidence of past negative interactions. The objective of our study was to describe the presence of scars in an elephant population in the Greater Tsavo Ecosystem, a region of high human–elephant conflict in Kenya. We monitored and catalogued a population in the Kasigau Wildlife Corridor from 2017 to 2021, noting the number of scars and their placement on individuals. Presumably, evidence of conflict between elephants is more likely to be found on the head or rump areas of elephants, whereas humans are most likely to injure the body of elephants. We found that adult males were much more likely to have scars than adult females, older males were more likely to have scars than younger males, and more scars were located on the body as opposed to the rump and head. Understanding how many elephants in a population have scars and the location of scars can describe the potential level of human–elephant conflict in a population. This valuable information can be used to better understand the level of conflict in a community and aid in management recommendations. Human–elephant conflict (HEC) compromises crop security and threatens elephant conservation. Most commonly, HEC manifests as crop-foraging as elephants modify natural foraging strategies to incorporate crops. Farmers may retaliate by frightening or harming elephants, leaving scars from inflicted wounds. We assessed the prevalence and distribution of scars on the bodies of African savanna elephants (Loxodonta africana) observed in the Kasigau Wildlife Corridor (KWC), part of the Greater Tsavo Ecosystem of Kenya, where conflict is prevalent. We surmised that scars on the body are largely a result of HEC as opposed to scars on the rump or head, which we attributed primarily to elephant–elephant conflict. We hypothesized that: (1) male elephants would have more scars than females; (2) older males would be more likely to have scars than younger males; and (3) most scars would be located on the bodies of elephants. We assessed scars from a photographic catalogue of elephants from the KWC. In line with our hypotheses, male elephants were more likely to have scars than females (32% of males compared to 6% of females); older males had significantly more scars than younger males (61% compared to 24%); and the majority of scars (89%) were located on the body. Scar presence may be useful as an animal-centered indicator to estimate the prevalence and demographic patterns of HEC. [ABSTRACT FROM AUTHOR]

Elephants influence the structure and composition of African woodlands and, sometimes, damage cultivated trees in farms of local communities. Here, we evaluate the impact of elephant debarking on cultivated cashew trees (Anacardium occidentale) as the friction between farmers and elephant conservation is increasing. We quantified the effects of elephant debarking on tree survival, crown defoliation, and nut production by visiting elephant-damaged farms with cashew trees (n = 47 farms) along Sasawara National Forest (Tanzania). We also compared defoliation and nut production with nearby control trees (undamaged) in a paired designed. Our results reveal that elephants debarked a significant proportion of trees (36% of farms had some cashew trees damaged by elephants, with a mean of 17.3% damaged trees per farm), with a preference for medium-large tree sizes (> 20 cm of diameter at breast height). Tree mortality due to elephants was 4 trees (4.2% of the affected trees), which represents around 0.1% of the total number of trees in the affected farms. In the year of damage, elephant debarking caused no effect on defoliation whereas nut production was reduced only in trees with very high intensity of damage (> 60% of trunk circumference affected). However, a year after the damage, both defoliation and nut production were negatively affected but this effect was strongly dependent on the intensity of damage and tree diameter. These results may help reduce the social discomfort of cashew farmers and contribute to enhance human-elephant coexistence. However, further studies should analyze the future vulnerability of damaged trees to fires, pathogens or pests. [ABSTRACT FROM AUTHOR]

Elephant endotheliotropic herpesvirus (EEHV) infection can cause acute, often fatal, EEHV hemorrhagic disease in free-ranging and human-managed Asian elephants (Elephas maximus) and human-managed African elephants (Loxodonta africana). However, significant knowledge gaps exist pertaining to the presence of EEHV in free-ranging African elephant populations. We retrospectively screened 142 opportunistically collected samples (blood, n=98; bronchoalveolar lavage (BAL) fluid, n=21; trunk wash (TW) fluid, n=23) obtained between 2010 and 2020 from 98 free-ranging African elephants in the Kruger National Park, South Africa, for the presence of different EEHVs, as well as determining the real-time quantitative PCR positivity rate in this population. With the use of validated, previously published DNA extraction and real-time quantitative PCR protocols provided by the National Elephant Herpesvirus Laboratory (Washington, DC, USA), EEHV was detected in nine male African elephants from samples collected in 2011 (n=1), 2013 (n=1), 2018 (n=2), 2019 (n=4), and 2020 (n=1). Viral detection was more common in respiratory compared with blood samples. Six elephants tested positive for EEHV2 subtype (blood, n=2; BAL, n=3; TW, n=2), including one individual that tested positive on matched respiratory samples (BAL and TW). Four elephants tested positive for EEHV3-4-7 (blood, n=1; BAL, n=2; TW, n=1), whereas EEHV6 was not detected in any of the study animals. One elephant tested positive for both EEHV2 and EEHV3-4-7 in the same BAL sample. Even though the levels of viremia varied between 158 and 1,292 viral genome equivalents/mL blood and viral shedding of EEHV2 and EEHV3-4-7 was detected in respiratory samples, no clinical signs were observed in these apparently healthy elephants. These findings are consistent with reports of asymptomatic EEHV infection in human-managed African elephants. [ABSTRACT FROM AUTHOR]

This study compared hematocrit measured with the EPOC and i-STAT Alinity v point-of-care analyzers and manual measurement of packed cell volume in managed African savanna elephants (Loxodonta africana) and southern white rhinoceros (Ceratotherium simum simum). Biochemical analytes were also measured with the EPOC, i-STAT Alinity v, and a veterinary diagnostic laboratory in the same animals. Analytes assessed included blood urea nitrogen, chloride, creatinine, glucose, ionized calcium, potassium, and sodium. There were no differences for hematocrit values for African savanna elephants or southern white rhinoceros (p ≤ 0.05). In African savanna elephants, there were no differences between the EPOC and i-STAT Alinity v analyzers for any measured analytes except ionized calcium. When compared to a veterinary diagnostic laboratory, there were differences for a majority of the biochemical analytes measured on the EPOC and i-STAT Alinity v analyzers in African savanna elephants. In southern white rhinoceros, there were differences for a majority of analytes among all three analyzers. While differences existed among the portable analyzers and a veterinary diagnostic laboratory for biochemical analytes in both species, these numerically small differences are unlikely to be clinically significant. For routine health care of African savanna elephants and southern white rhinoceros, these point-of-care analyzers may be a useful alternative to commercial analyzers for the parameters evaluated. [ABSTRACT FROM AUTHOR]

Consumers, including megaherbivores and fire, are considered important limiting forces for woody plants and canopy closure in African savannas. However, climatic events like drought can also play a significant role in limiting trees and maintaining tree‐grass coexistence in savannas. The extent to which top‐down control (e.g. megaherbivores) and bottom‐up resource limitation through drought and competition interact to influence savanna tree mortality and woody structure is unclear. Here, we compared the change in the number of large trees before and after a severe drought in a savanna with elephants (Loxodonta africana) and one without elephants. Elephants and drought both limited the number of large trees at our sites, but contrary to our predictions, there was no interactive effect of these drivers on overall changes in tree densities. However, there was a synergistic effect on the dominant tree species, Senegalia nigrescens, such that tree loss post‐drought was greater where elephants were present compared to where they were absent. Hence, our results suggest that species‐specific differences in drought resistance, as well as density‐dependent factors, likely impact the severity of drought effects on savanna tree communities. In savannas, drought has the potential to exert strong control on tree survival and prevent canopy closure, thus partially filling the role of megaherbivores in limiting large trees when these consumers are absent. As drought severity and frequency are predicted to increase in the future, the influence of drought on savanna vegetation structure becomes increasingly important to consider. [ABSTRACT FROM AUTHOR]

The African savannah ecosystem is populated by nomadic pastoralists who herd livestock in the day and corral them at night in temporary enclosures, called bomas, to protect them. The number and distribution of bomas on the savannah is important from an ecological perspective and may have a significant impact on wildlife movement. However, no study has yet examined this relationship. Here, using very high‐resolution satellite imagery from two time periods, we quanitified changes in boma distribution and density across an area of 3377 km2 in the Laikipia‐Samburu ecosystem of northern Kenya between 2011 and 2019. To assess wildlife movement in relation to bomas, we used a GPS data set on African bush elephant Loxodonta africana movement from 27 collared matriarchs representing herds of 9–15, covering 112 467 hourly GPS fixes over 31 months between 2018 and 2020. Our results showed a more than 46% increase in the total number of human‐built structures between 2011 and 2019, the majority of which were bomas, representing a 21.9% increase in human‐modified land area. Elephants readily adjusted their foraging habits and itineraries in this habitat shared with humans, who were also nomadic in space and time. Assessing the night–day activity ratio, we found elephants move more nocturnally when in closer proximity to bomas, particularly during the dry season. This temporal separation means elephants avoid the times humans are active in and around bomas while still accessing required resources—water and forage. The temporal shift was stronger during the dry season when shared resources are scarce. Using daily travel distance as a metric, we show elephants moved further in closer proximity to bomas which was likely linked to the need to travel between forage patches. Given the rise in human settlements, understanding the consequences of animals' behavioral adjustments is critical to understand the long‐term population viability of elephant populations. [ABSTRACT FROM AUTHOR]

Unique features of elephant hematology are known challenges in analytical methodology like two types of monocytes typical for members of the Order Afrotheria and platelet counts of the comparatively small elephant platelet. To investigate WBC differential and platelet data generated by an impedance-based hematology analyzer without availability of validated species-specific software for recognition of elephant WBCs and platelets, compared to manual blood film review. Blood samples preserved in ethylenediaminetetraacetic acid (EDTA) of 50 elephants (n = 35 Elephas maximus and n = 15 Loxodonta africana) were used. A Mann-Whitney test for independent samples was used to compare parameters between methods and agreement was tested using Bland-Altman bias plots. All hematological variables, including absolute numbers of heterophils, lymphocytes, monocytes, eosinophils, basophils, and platelets, were significantly different (p

Abstract The influence of environmental factors on the distribution and persistence of African elephants (Loxodonta africana) is pertinent to policy makers and managers to formulate balanced plans for different land‐use types. The study focuses on movement of elephants and how they utilize foraging areas in Sioma Ngwezi landscape in Zambia by answering the following questions: (1) Which environmental variables and land‐cover class predict the movement of elephants during the wet season in Sioma Ngwezi landscape? (2) What is the wet season suitable habitat for elephants in Sioma Ngwezi landscape? (3) What are the major wet season movement corridors for elephants in Sioma Ngwezi landscape? We used GPS telemetry data from the collared elephants to assess habitat connectivity. Maximum entropy (MaxEnt) and linkage mapper were the tools used to predict habitat suitability, movement corridors, and barriers in the landscape during the wet season. The study identified elevation, land cover, and NDVI as the most important environmental predictors that modify the dispersal of elephants in the landscape during the wet season. Additionally, a total of 36 potential wet season corridors were identified connecting 15 core areas mainly used for foraging and protection from poachers in the landscape. Of these, 24 corridors were highly utilized and are suggested as priority corridors for elephant movement in the landscape. The identified wet season habitats and functional corridors may help to combat elephant poaching by patrolling areas with high relative probability of elephant presence. The findings may also help abate human–elephant conflict such as crop‐raiding by managing identified corridors that run into agriculture zones in the game management area.

Abstract The majority of Africa's parks and conservation areas have a vast road network, facilitating motorized vehicle game viewing. These roads have an influence that is both road type‐ and species‐specific, on the surrounding ecosystem. Due to their higher traffic volumes, we hypothesized that tar roads and their immediate surrounds within the Kruger National Park, South Africa, are avoided to a greater extent by medium‐to‐large mammals than comparable dirt roads in the park. We systematically recorded the presence of medium‐to‐large mammal species from our vehicle, recording data at 401 tar and 369 dirt road stops in the Kruger National Park. In addition to species presence, we also estimated the proximity of animals to the road, as well as herd sizes. Our results indicate an equal likelihood of viewing the commonly recorded medium‐to‐large mammal species from both road types. The likelihood of observing larger herd sizes was also similar between tar and dirt roads for the three most commonly observed species, African elephant (Loxodonta africana), impala (Aepyceros melampus), and plains zebra (Equus quagga), and the likelihood of viewing impala and zebra close to the road also did not differ between tar and dirt roads. However, elephant was observed more often close to tar roads, compared to dirt roads. We interpreted this as the result of potentially increased woody cover associated with more water runoff in close proximity to tar roads compared with dirt roads. Our results not only have ecological significance, supporting the notion that many of the park's species are habituated to human infrastructure, but also management implications, informing park officials about the influence of road traffic and road type on wildlife distributions.

Chemical communication between sexes in the elephants has been well studied at the chemical and behavioural levels, but little is known about the proteins mediating the exchange of chemical signals. Two sex pheromones have been identified in Asian elephants: (Z)-7-dodecenyl acetate and frontalin, and their effects on the elephants’ behaviour have been described in detail. The genomes of both the Asian (Elephas maximus) and the African elephant (Loxodonta africana) have been poorly annotated. In particular, the complete sequences of two odorant-binding proteins and a VEG protein are available for the African elephant, together with isoforms and other members of the same families, which however are incomplete or unreliable. In a previous study, we have expressed the OBP1 of both elephant species, and investigated their binding properties. We showed that OBP1 is tuned to the pheromone (Z)-7-dodecenyl acetate and few structurally related linear esters, but also binds (E)-β-farnesene and farnesol with good affinity. In this work we have explored the characteristics of the second OBP of the African elephant (LafrOBP2). This protein, which was not found in the trunk wash, does not bind any of the above listed semiochemicals. Instead, it shows selected affinity to unsaturated linear aldehydes of 16 carbon atoms, specifically (Z)-9-hexadecenal, (Z)-11-hexadecenal and (10E,12Z)-hexadecadienal (bombykal). Fourteen and 18 carbon orthologues show only much reduced binding affinity. Some linear alcohols, fatty acids and esters also weakly bind this protein with dissociation constants about one order of magnitude higher.

African zoo elephants live in safe environments with sufficient resources, are protected from threats, and have their health and body conditions cared for. Calves ex situ undergo the same developmental stages as in situ and are raised by the whole family unit. However, due to environmental differences, there might be behavioral modifications between calves in situ and ex situ. We hypothesize that these differences increase with ongoing generations. This ethological study compares social and general behavior and the distance calves kept to their mothers’ between calves of the first (F1) and second (F2) zoo generation and the wild. Using ethological methods, data were collected for ~90 in situ calves and 16 ex situ (8 F1, 8 F2) between the ages of 0.5 to 4 years (120 observation hours per group). Results showed that in situ calves spent significantly more time close to mothers than the F1 and the F2 zoo generations (F1/in situ: p = p = 0.007). The behaviors of eating, drinking, trunk movement, washing, and affiliative behaviors showed significant differences between in situ and ex situ calves. The amount and distribution of affiliative and agonistic behavior initiated and received by calves was displayed with a greater variety ex situ. Ex situ calves not only performed affiliative but, in contrast to the in situ, also agonistic behavior (F1/in situ: initiated p = 0.002, received p = 0.010; F2/in situ: initiated p = 0.050, received p = 0.037). The comparison of zoo generations suggests that differences did not increase with the generation. The more casual binding between mothers and offspring in zoos and the age-dependent improvement of social behavior of zoo-born calves are seen as a result of elephants’ adaptation to secure zoo conditions. The results of this study agree with the faster development of ex situ African elephants, like earlier puberty and more frequent breeding patterns, as known from the literature.

Following population declines of the African savanna elephant (Loxodonta africana) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene TP53. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (n = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.