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130 results on '"Low, Chian-Ming"'

1. SYNAPSE: An international roadmap to large brain imaging

Author

Stampfl, Anton P.J., Liu, Zhongdong, Hu, Jun, Sawada, Kei, Takano, H., Kohmura, Yoshiki, Ishikawa, Tetsuya, Lim, Jae-Hong, Je, Jung-Ho, Low, Chian-Ming, Teo, Alvin, Tok, Eng Soon, Tan, Tin Wee, Ban, Kenneth, Libedinsky, Camilo, Tan, Francis Chee Kuan, Chen, Kuan-Peng, Yang, An-Cheng, Chuang, Chao-Chun, Chen, Nan-Yow, Shih, Chi-Tin, Lee, Ting-Kuo, Yang, De-Nian, Lai, Hsu-Chao, Shuai, Hong-Han, Cheng, Chang-Chieh, Ching, Yu-Tai, Li, Chia-Wei, Charng, Ching-Che, Lo, Chung-Chuan, Chiang, Ann-Shyn, Recur, Benoit, Petibois, Cyril, Cheng, Chia-Liang, Chen, Hsiang-Hsin, Yang, Shun-Min, Hwu, Yeukuang, Rojviriya, Catleya, Rugmai, Supagorn, Rujirawat, Saroj, and Margaritondo, Giorgio

Published
2023
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2. The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution

Author

Kohmura, Yoshiki, Yang, Shun-Min, Chen, Hsiang-Hsin, Takano, Hidekazu, Chang, Chia-Ju, Wang, Ya-Sian, Lee, Tsung-Tse, Chiu, Ching-Yu, Yang, Kai-En, Chien, Yu-Ting, Hu, Huan-Ming, Su, Tzu-Ling, Petibois, Cyril, Chen, Yi-Yun, Hsu, Cheng-Huan, Chen, Peilin, Hueng, Dueng-Yuan, Chen, Shean-Jen, Yang, Chi Lin, Chin, An-Lun, Low, Chian-Ming, Tan, Francis Chee Kuan, Teo, Alvin, Tok, Eng Soon, Cai, Xu Xiang, Lin, Hong-Ming, Boeckl, John, Stampfl, Anton P., Yamada, Jumpei, Matsuyama, Satoshi, Ishikawa, Tetsuya, Margaritondo, Giorgio, Chiang, Ann-Shyn, and Hwu, Yeukuang

Published
2022
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3. A synchrotron X-ray imaging strategy to map large animal brains

Author

Chin, An-Lun, Yang, Shun-Min, Chen, Hsiang-Hsin, Li, Min-Tsang, Lee, Tsung-Tse, Chen, Ying-Jie, Lee, Ting-Kuo, Petibois, Cyril, Cai, Xiaoqing, Low, Chian-Ming, Tan, Francis Chee Kuan, Teo, Alvin, Tok, Eng Soon, Ong, Edwin B.L., Lin, Yen-Yin, Lin, I-Jin, Tseng, Yi-Chi, Chen, Nan-Yow, Shih, Chi-Tin, Lim, Jae-Hong, Lim, Jun, Je, Jung-Ho, Kohmura, Yoshiki, Ishikawa, Tetsuya, Margaritondo, Giorgio, Chiang, Ann-Shyn, and Hwu, Yeukuang

Published
2020
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6. SYNAPSE: An international roadmap to large brain imaging

Author

Stampfl, Anton P. J., Liu, Zhongdong, Hu, Jun, Sawada, Kei, Takano, H., Kohmura, Yoshiki, Ishikawa, Tetsuya, Lim, Jae-Hong, Je, Jung-Ho, Low, Chian-Ming, Teo, Alvin, Tok, Eng Soon, Tan, Tin Wee, Ban, Kenneth, Libedinsky, Camilo, Tan, Francis Chee Kuan, Chen, Kuan-Peng, Yang, An-Cheng, Chuang, Chao-Chun, Chen, Nan-Yow, Shih, Chi-Tin, Lee, Ting-Kuo, Yang, De-Nian, Lai, Hsu-Chao, Shuai, Hong-Han, Cheng, Chang-Chieh, Ching, Yu-Tai, Li, Chia-Wei, Charng, Ching-Che, Lo, Chung-Chuan, Chiang, Ann-Shyn, Recur, Benoit, Petibois, Cyril, Cheng, Chia-Liang, Chen, Hsiang-Hsin, Yang, Shun-Min, Hwu, Yeukuang, Rojviriya, Catleya, Rugmai, Supagorn, Rujirawat, Saroj, Margaritondo, Giorgio, Stampfl, Anton P. J., Liu, Zhongdong, Hu, Jun, Sawada, Kei, Takano, H., Kohmura, Yoshiki, Ishikawa, Tetsuya, Lim, Jae-Hong, Je, Jung-Ho, Low, Chian-Ming, Teo, Alvin, Tok, Eng Soon, Tan, Tin Wee, Ban, Kenneth, Libedinsky, Camilo, Tan, Francis Chee Kuan, Chen, Kuan-Peng, Yang, An-Cheng, Chuang, Chao-Chun, Chen, Nan-Yow, Shih, Chi-Tin, Lee, Ting-Kuo, Yang, De-Nian, Lai, Hsu-Chao, Shuai, Hong-Han, Cheng, Chang-Chieh, Ching, Yu-Tai, Li, Chia-Wei, Charng, Ching-Che, Lo, Chung-Chuan, Chiang, Ann-Shyn, Recur, Benoit, Petibois, Cyril, Cheng, Chia-Liang, Chen, Hsiang-Hsin, Yang, Shun-Min, Hwu, Yeukuang, Rojviriya, Catleya, Rugmai, Supagorn, Rujirawat, Saroj, and Margaritondo, Giorgio

Abstract

Since 2020, synchrotron radiation facilities in several Asia-Pacific countries have been collaborating in a major project called "SYNAPSE"(Synchrotrons for Neuroscience: an Asia-Pacific Scientific Enterprise). They use x-ray imaging to attack in a coordinated fashion one of the major issues in modern science: the structure of animal and human brains, including neurons and connections. The objective is to develop Google-like maps also including detailed structural and functional information for selected regions of interest. The sheer mass of data needed for the objective poses huge problems for the acquisition, processing, storage and use of images. In order to complete the task within a reasonable time, the key element of the SYNAPSE strategy is the parallel and coordinated work of several facilities on the same specimens. This article reviews different aspects of the enterprise, including the foundations of synchrotron radiation, coherence and of its role in advanced imaging, electron accelerators, x-ray optics and detectors. This will provide the foundation for an extensive presentation of the different components of SYNAPSE, with an overview of results already obtained within the consortium.

Published
2023
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10. Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels

Author

Hansen, Kasper B., primary, Wollmuth, Lonnie P., additional, Bowie, Derek, additional, Furukawa, Hiro, additional, Menniti, Frank S., additional, Sobolevsky, Alexander I., additional, Swanson, Geoffrey T., additional, Swanger, Sharon A., additional, Greger, Ingo H., additional, Nakagawa, Terunaga, additional, McBain, Chris J., additional, Jayaraman, Vasanthi, additional, Low, Chian-Ming, additional, Dell’Acqua, Mark L., additional, Diamond, Jeffrey S., additional, Camp, Chad R., additional, Perszyk, Riley E., additional, Yuan, Hongjie, additional, and Traynelis, Stephen F., additional

Published
2021
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11. High-resolution fast-tomography brain-imaging beamline at the Taiwan Photon Source

Author

Chen, Hsiang Hsin, primary, Yang, Shun-Min, additional, Yang, Kai-En, additional, Chiu, Ching-Yu, additional, Chang, Chia-Ju, additional, Wang, Ya-Sian, additional, Lee, Tsung-Tse, additional, Huang, Yu-Fen, additional, Chen, Yi-Yun, additional, Petibois, Cyril, additional, Chang, Shih-Hung, additional, Cai, Xiaoqing, additional, Low, Chian-Ming, additional, Tan, Francis Chee Kuan, additional, Teo, Alvin, additional, Tok, Eng Soon, additional, Lim, Jae-Hong, additional, Je, Jun-Ho, additional, Kohmura, Yoshiki, additional, Ishikawa, Tetsuya, additional, Margaritondo, Giorgio, additional, and Hwu, Yeukuang, additional

Published
2021
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20. Structural insights into phenylethanolamines high-affinity binding site in NR2B from binding and molecular modeling studies

Author

Ng Fui-Mee, Geballe Matthew T, Snyder James P, Traynelis Stephen F, and Low Chian-Ming

Subjects
Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Background Phenylethanolamines selectively bind to NR2B subunit-containing N-methyl-D-aspartate-subtype of ionotropic glutamate receptors and negatively modulate receptor activity. To investigate the structural and functional properties of the ifenprodil binding domain on the NR2B protein, we have purified a soluble recombinant rat NR2B protein fragment comprising the first ~400 amino acid amino-terminal domain (ATD2B) expressed in E. coli. Spectral measurements on refolded ATD2B protein demonstrated specific binding to ifenprodil. We have used site-directed mutagenesis, circular dichroism spectroscopy and molecular modeling to obtain structural information on the interactions between critical amino acid residues and ifenprodil of our soluble refolded ATD2B proteins. Ligand-induced changes in protein structure were inferred from changes in the circular dichroism spectrum, and the concentration dependence of these changes was used to determine binding constants for ifenprodil and its analogues. Results Ligand binding of ifenprodil, RO25,6981 and haloperidol on soluble recombinant ATD2B determined from circular dichroism spectroscopy yielded low-to-high micromolar equilibrium constants which concurred with functional IC50 measurement determined in heterologously expressed NR1/NR2B receptors in Xenopus oocytes. Amino acid residue substitutions of Asp101, Ile150 and Phe176 with alanine residue within the ATD2B protein altered the recombinant protein dissociation constants for ifenprodil, mirroring the pattern of their functional phenotypes. Molecular modeling of ATD2B as a clam-shell-like structure places these critical residues near a putative ligand binding site. Conclusion We report for the first time biochemical measurements show that the functional measurements actually reflect binding to the ATD of NR2B subunit. Insights gained from this study help advance the theory that ifenprodil is a ligand for the ATD of NR2B subunit.

Published
2008
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22. A new generation of arachidonic acid analogues targeting cytosolic phospholipase A2

Author

Srinivasaraghavan Kannan, Ong Wee Yong, Go Mei Lin, Chen Yong Jun, Low Chian-Ming, Tan Francis Chee Kuan, Yulin Lam, Ng Cheng Yang, and S Verma Chandra

Subjects
Cytosol, chemistry.chemical_compound, Phospholipase A2, biology, Biochemistry, Chemistry, Applied Mathematics, General Mathematics, biology.protein, Arachidonic acid, Neuroinflammation
Published
2018

23. Curcumin Ameliorates Neuroinflammation, Neurodegeneration, and Memory Deficits in p25 Transgenic Mouse Model that Bears Hallmarks of Alzheimer’s Disease

Author

Sundaram, Jeyapriya Raja, primary, Poore, Charlene Priscilla, additional, Sulaimee, Noor Hazim Bin, additional, Pareek, Tej, additional, Cheong, Wei Fun, additional, Wenk, Markus R., additional, Pant, Harish C., additional, Frautschy, Sally A., additional, Low, Chian-Ming, additional, and Kesavapany, Sashi, additional

Published
2017
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27. GluN2D-Containing N-methyl-d-Aspartate Receptors Mediate Synaptic Transmission in Hippocampal Interneurons and Regulate Interneuron Activity

Author

Perszyk, Riley E., primary, DiRaddo, John O., additional, Strong, Katie L., additional, Low, Chian-Ming, additional, Ogden, Kevin K., additional, Khatri, Alpa, additional, Vargish, Geoffrey A., additional, Pelkey, Kenneth A., additional, Tricoire, Ludovic, additional, Liotta, Dennis C., additional, Smith, Yoland, additional, McBain, Chris J., additional, and Traynelis, Stephen F., additional

Published
2016
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29. Cleavage of the NR2B Subunit Amino Terminus of N-Methyl-d-aspartate (NMDA) Receptor by Tissue Plasminogen Activator: IDENTIFICATION OF THE CLEAVAGE SITE AND CHARACTERIZATION OF IFENPRODIL AND GLYCINE AFFINITIES ON TRUNCATED NMDA RECEPTOR*

Author

Ng, Kay-Siong, Leung, How-Wing, Wong, Peter T.-H., and Low, Chian-Ming

Subjects
Male, Time Factors, integumentary system, Dose-Response Relationship, Drug, Glycine, Glycine Agents, Receptors, N-Methyl-D-Aspartate, Recombinant Proteins, Protein Structure, Tertiary, Rats, Rats, Sprague-Dawley, Neurobiology, Piperidines, Sequence Analysis, Protein, Tissue Plasminogen Activator, Proteolysis, Mutagenesis, Site-Directed, Animals, Humans, Excitatory Amino Acid Antagonists
Abstract

Thrombolysis using tissue plasminogen activator (tPA) has been the key treatment for patients with acute ischemic stroke for the past decade. Recent studies, however, suggest that this clot-busting protease also plays various roles in brain physiological and pathophysiological glutamatergic-dependent processes, such as synaptic plasticity and neurodegeneration. In addition, increasing evidence implicates tPA as an important neuromodulator of the N-methyl-d-aspartate (NMDA) receptors. Here, we demonstrate that recombinant human tPA cleaves the NR2B subunit of NMDA receptor. Analysis of NR2B in rat brain lysates and cortical neurons treated with tPA revealed concentration- and time-dependent degradation of NR2B proteins. Peptide sequencing studies performed on the cleaved-off products obtained from the tPA treatment on a recombinant fusion protein of the amino-terminal domain of NR2B revealed that tPA-mediated cleavage occurred at arginine 67 (Arg(67)). This cleavage is tPA-specific, plasmin-independent, and removes a predicted ~4-kDa fragment (Arg(27)-Arg(67)) from the amino-terminal domain of the NR2B protein. Site-directed mutagenesis of putative cleavage site Arg(67) to Ala(67) impeded tPA-mediated degradation of recombinant protein. This analysis revealed that NR2B is a novel substrate of tPA and suggested that an Arg(27)-Arg(67)-truncated NR2B-containing NMDA receptor could be formed. Heterologous expression of NR2B with Gln(29)-Arg(67) deleted is functional but exhibits reduced ifenprodil inhibition and increased glycine EC(50) with no change in glutamate EC(50). Our results confirmed NR2B as a novel proteolytic substrate of tPA, where tPA may directly interact with NR2B subunits leading to a change in pharmacological properties of NR2B-containing NMDA receptors.

Published
2012

32. Fluorogenic probes to monitor cytosolic phospholipase A2 activity.

Author

Ng, Cheng Yang, Kwok, Timothy Xiong Wei, Tan, Francis Chee Kuan, Low, Chian-Ming, and Lam, Yulin

Subjects
FLUORESCENT probes, ARACHIDONIC acid derivatives, PHOSPHOLIPASE A2
Abstract

Arachidonic acid derivatives equipped with either one or two fluorescent groups attached to the tip of the alkyl chains were synthesized and shown to function as inhibitor and substrate probes of cPLA2. The inhibitor probe was demonstrated to perform dual functions of inhibition and imaging while the substrate probe could be used for activity assay. [ABSTRACT FROM AUTHOR]

Published
2017
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47. Molecular Determinants of Proton-Sensitive N-Methyl-d-aspartate Receptor Gating

Author

Low, Chian-Ming, primary, Lyuboslavsky, Polina, additional, French, Adam, additional, Le, Phuong, additional, Wyatte, Karen, additional, Thiel, William H., additional, Marchan, Edward M., additional, Igarashi, Kazuei, additional, Kashiwagi, Keiko, additional, Gernert, Kim, additional, Williams, Keith, additional, Traynelis, Stephen F., additional, and Zheng, Fang, additional

Published
2003
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49. A synchrotron X-ray imaging strategy to map large animal brains

Author

Chin, An-Lun, Yang, Shun-Min, Chen, Hsiang-Hsin, Li, Min-Tsang, Lee, Tsung-Tse, Chen, Ying-Jie, Lee, Ting-Kuo, Petibois, Cyril, Cai, Xiaoqing, Low, Chian-Ming, Tan, Francis Chee Kuan, Teo, Alvin, Tok, Eng Soon, Ong, Edwin B. L., Lin, Yen-Yin, Lin, I-Jin, Tseng, Yi-Chi, Chen, Nan-Yow, Shih, Chi-Tin, Lim, Jae-Hong, Lim, Jun, Je, Jung-Ho, Kohmura, Yoshiki, Ishikawa, Tetsuya, Margaritondo, Giorgio, Chiang, Ann-Shyn, and Hwu, Yeukuang

Subjects
microtomography, nanotomography, neural network, connectome, diffusion, x-ray imaging, microscopy, brain imaging, tomography, light
Abstract

Mapping the large neural networks of animal and human brains is a fundamental but so far elusive task, because of the massive amount of data and the consequent prohibitively long image taking and processing times. We developed an effective strategy called "AXON" (Accelerated X-ray Observation of Neurons) to solve this problem. AXON can achieve comprehensive whole-brain mapping within a reasonable time by combining fast image taking and processing, plus two other critical performances: three-dimensional (3D) imaging with high and isotropic spatial resolution, and multi-scale resolution. We successfully tested this strategy with coordinated experiments at four synchrotron facilities in Japan, Taiwan, Singapore and Korea on two animal models, Drosophila and mouse. Its performances notably allowed full 3D mapping of the Drosophila brain in a few days. With reasonable improvements, AXON can deliver full mapping of large animal and human brains on a realistic time scale of a few years.

50. Fluorogenic probes to monitor cytosolic phospholipase A 2 activity.

Author

Ng CY, Kwok TX, Tan FC, Low CM, and Lam Y

Subjects
Arachidonic Acid metabolism, Cell Line, Fluorescent Dyes analysis, Fluorescent Dyes chemical synthesis, Humans, Molecular Structure, Cytosol enzymology, Fluorescent Dyes chemistry, Phospholipases A2 analysis, Phospholipases A2 metabolism
Abstract

Arachidonic acid derivatives equipped with either one or two fluorescent groups attached to the tip of the alkyl chains were synthesized and shown to function as inhibitor and substrate probes of cPLA 2 . The inhibitor probe was demonstrated to perform dual functions of inhibition and imaging while the substrate probe could be used for activity assay.

Published
2017
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