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Abstract Following an application from DuPont Nutrition Biosciences ApS submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Ireland, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to Bifidobacterium animalis subsp. lactis Bi‐07 (Bi‐07) and contribution to increasing lactose digestion. The scope of the application was proposed to fall under a health claim based on newly developed scientific evidence. The food proposed by the applicant as the subject of the health claim is Bi‐07. The Panel considers that Bi‐07 is sufficiently characterised. The claimed effect proposed by the applicant is ‘improvement of lactose digestion’. The Panel considers that increasing lactose digestion is a beneficial physiological effect for individuals with lactose maldigestion provided that the symptoms of lactose maldigestion are improved. Two human intervention studies which investigated a single dose effect of Bi‐07 on lactose digestion using the hydrogen breath test, as well as on gastrointestinal symptoms were submitted. These studies show that consumption of Bi‐07 (1012 CFU) increases lactose digestion in individuals with lactose maldigestion and that Bi‐07 exhibits lactase activity in vitro. However, these studies provide no evidence that increasing lactose digestion through the consumption of Bi‐07 (1012 CFU) improves gastrointestinal symptoms of lactose maldigestion, which is considered a beneficial physiological effect. The Panel concludes that a cause and effect relationship has not been established between the consumption of Bifidobacterium animalis subsp. lactis Bi‐07 and a beneficial physiological effect (i.e. the improvement of symptoms of lactose maldigestion) in individuals with lactose maldigestion.

Novel metabolites of the anabolic androgenic steroid 17α-methyltestosterone have been detected in HepG2 cell in vitro metabolic model and in human urine. Their detection was accomplished through targeted gas chromatography-(tandem) mass spectrometry analysis that has been based on microscale synthesized standards. The related synthesis and the gas chromatography-(tandem) mass spectrometry characterization of the analytical standards are described. All newly presented metabolites have a fully reduced steroid A-ring with either an 17,17-dimethyl-18-nor-Δ13 structure or they have been further oxidized at position 16 of the steroid backbone. Metabolites with 17,17-dimethyl-18-nor-Δ13 structure may be considered as side products of phase II metabolic sulfation of the 17β-hydroxy group of methyltestosterone or its reduced tetrahydro-methyltestosterone metabolites 17α-methyl-5β-androstane-3α,17β-diol and 17α-methyl-5α-androstane-3α,17β-diol that produce the known epimeric 17β-methyl-5β-androstane-3α,17α-diol and 17β-methyl-5α-androstane-3α,17α-diol metabolites. The prospective of these new metabolites to increase detection time windows and improve identification was investigated by applying the World Anti-doping Agency TD2021IDCR criteria. The new metabolites, presented herein, complement the current knowledge on the 17α-methyltestosterone metabolism and in some cases can be used as additional long-term markers in the frame of sport doping drug testing., (© 2023 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.)

The present study examined differences between inflectional and derivational morphology using Greek nouns and verbs with masked priming (with both short and long stimulus onset asynchrony) and long-lag priming. A lexical decision task to inflected noun and verb targets was used to test whether their processing is differentially facilitated by prior presentation of their stem in words of the same grammatical class (inflectional morphology) or of a different grammatical class (derivational morphology). Differences in semantics, syntactic information, and morphological complexity between inflected and derived word pairs (both nouns and verbs) were minimized by unusually tight control of stimuli as permitted by Greek morphology. Results showed that morphological relations affected processing of morphologically complex Greek words (nouns and verbs) across prime durations (50-250ms) as well as when items intervened between primes and targets. In two of the four experiments (Experiments 1 and 3), inflectionally related primes produced significantly greater effects than derivationally related primes suggesting differences in processing inflectional versus derivational morphological relations, which may disappear when processing is less dependent on semantic effects (Experiment 4). Priming effects differed for verb vs. noun targets with long SOA priming (Experiment 3), consistent with processing differences between complex words of different grammatical class (nouns and verbs) when semantic effects are maximized. Taken together, results demonstrate that inflectional and derivational relations differentially affect processing complex words of different grammatical class (nouns and verbs). This finding indicates that distinctions of morphological relation (inflectional vs. derivational) are not of the same kind as distinctions of grammatical class (nouns vs. verbs). Asymmetric differences among inflected and derived verbs and nouns seem to depend on semantic effects and/or processing demands modulating priming effects very early in lexical processing of morphologically complex written words, consistent with models of lexical processing positing early access to morphological structure and early influence of semantics., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Loui, Protopapas and Orfanidou.)