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Cemeteries from the European Romantic period (18th–19th centuries) are often compared to small cities that hold memories, art, and history. Portuguese public cemeteries were first established in 1835 and became an interesting combination of fauna, flora, and monumental sculptures to mourn the dead at a location outside the limits of the city. Over the past 187 years, laws have been created and amended taking into consideration the needs of the population and the scientific knowledge available at each time point in history. Nevertheless, cemeteries have long been struggling with the lack of burial space which has been emphasised during the two years of the COVID pandemic. This work aims to review the development of Portuguese public cemeteries since their establishment, highlighting the imposed measures for the inhumation and exhumation of the deceased. It will also discuss the importance of soil as an abiotic agent, focusing on eight specific soil properties and their significance on the characterisation of graves. It is expected that a better understanding of the impact of soil on human taphonomy supports the role of city halls in managing public cemeteries, particularly the lack of burial space.

The first regional topsoil sampling campaign since 2011 maps elevated background concentrations of potentially toxic elements in soils across north-west Syria following more than a decade of conflict.

Soil trace evidence can be useful in criminal investigations. A homicide which had occurred in South Brazil been concluded through the courts with a guilty conviction. A spade with soil traces adhering to it was seized from the confessed killer's house, it having been established that it had been used to bury parts of the victim's body. In the context of this confession, it provided an opportunity to test a protocol of analysis and verify the potential of discriminate soil sample analysis in such case works. This allowed us to test the practice of sequential analysis which had been developed for forensic case works in Brazil, with three sequential extractions: (i) 0.2 mol/L pH 3.0 ammonium oxalate; (ii) dithionite-citrate-bicarbonate; and (iii) 0.5 mol/L NaOH. It was possible to predict the sequence of events related to the homicide by using the sequential extraction technique and to conclude that: (i) the A horizon soil from the burial location of the torso was found to be very similar to the soil samples which had been recovered from the spade, which was able to be established despite there only being a small amount of soil adhering to the spade; (ii) the location where the legs were buried contributed a low amount of soil adhering to the spade. Therefore, it is suggested that, where possible, sequential extractions should be prioritized from a questioned sample to best provide information about the likely sequence of contact places and this test likely scenarios and criminal events.

The forensic scenario, on which the round robin study was based, simulated a suspected intentional manipulation of a real estate rental agreement consisting of a total of three pages. The aims of this study were to (i) establish the amount and reliability of information extractable from a single type of evidence and to (ii) provide suggestions on the most suitable combination of compatible techniques for a multi-modal imaging approach to forgery detection. To address these aims, seventeen laboratories from sixteen countries were invited to answer the following tasks questions: (i) which printing technique was used? (ii) were the three pages printed with the same printer? (iii) were the three pages made from the same paper? (iv) were the three pages originally stapled? (v) were the headings and signatures written with the same ink? and (vi) were headings and signatures of the same age on all pages? The methods used were classified into the following categories: Optical spectroscopy, including multispectral imaging, smartphone mapping, UV-luminescence and LIBS ; Infrared spectroscopy, including Raman and FTIR (micro-)spectroscopy ; X-ray spectroscopy, including SEM-EDX, PIXE and XPS ; Mass spectrometry, including ICPMS, SIMS, MALDI and LDIMS ; Electrostatic imaging, as well as non- imaging methods, such as non-multimodal visual inspection, (micro-)spectroscopy, physical testing and thin layer chromatography. The performance of the techniques was evaluated as the proportion of discriminated sample pairs to all possible sample pairs. For the undiscriminated sample pairs, a distinction was made between undecidability and false positive claims. It was found that none of the methods used were able to solve all tasks completely and/or correctly and that certain methods were a priori judged unsuitable by the laboratories for some tasks. Correct results were generally achieved for the discrimination of printer toners, whereas incorrect results in the discrimination of inks. For the discrimination of paper, solid state analytical methods proved to be superior to mass spectrometric methods. None of the participating laboratories deemed addressing ink age feasible. It was concluded that correct forensic statements can only be achieved by the complementary application of different methods and that the classical approach of round robin studies to send standardised subsamples to the participants is not feasible for a true multimodal approach if the techniques are not available at one location.

To date, the only known occurrence of ambrein, an important perfumery organic molecule, is in coproliths found in about one in a hundred sperm whales. Jetsam ambergris coproliths from the whale are also found occasionally on beaches worldwide. Here we report on the surprising occurrence of ambrein in human adipocere. Adipocere is a waxy substance formed post-mortem during incomplete anaerobic decomposition of soft tissues. Adipocere samples obtained from grave exhumations were analysed using gas chromatography-mass spectrometry (GC-MS). In addition to the typical fatty acids of adipocere, lesser amounts of ambrein were identified in the samples, in abundances similar to those of the major accompanying faecal steroids. The distribution of these compounds suggests that ambrein was produced post-mortem during the microbial decomposition of faecal residues and tissues. It is assumed that the adipocere matrix of saturated fatty acidsaided the preservation of ambrein over extended periods of time, because adipocere is stable against degradation. The association of ambrein formation in ageing faecal material, under moist, oxygen-depleted conditions, now requires more attention in studies of other mammalian and geological samples. Indeed, ambrein and its transformation products may be useful novel chemical indicators of aged faecal matter and decomposed bodies.

Soil forensics utilizes extensive soil information to answer legal questions and test hypotheses. The main difficulty often is the determination of different variables from a small amount of soil sample collected on the suspect. We developed a sequential mineralogical and chemical analyses to assess a limited quantity of soil vestiges (0.5 g) from a suspect's vehicle (adhered to the outside rear-view mirror and to the left front fender) involved in a murder case and compared them with the surface samples found at the victim's body disposal site at the Graciosa Road, Parana State, Brazil. All results affirm that the suspect’s vehicle could have been in contact with the edge of the Graciosa Road, approximately the place where the victim’s body was located. As a result of the soil analysis and comparison, the results support the likely contact of the suspect’s vehicle with the crime scene.

International audience

Summary • This study examined morphological and topological responses of nodal root axes to defoliation in a fast- and a slow-growing grass species. • Vegetative tillers of both Lolium perenne and Festuca ovina were grown on slant boards and either left intact or subjected to repeated defoliation, under both a high nitrogen (N) and a low N supply. Root length, diameter and branching characteristics were measured on individual nodal root axes. • The total axis root length of F. ovina was less when plants had been defoliated. Root axis weight, primary root axis length and primary root diameter were also less with defoliation than an undefoliated control, under high N. Under low N conditions the root axes of F. ovina had a more randomly branched topology without defoliation. For L. perenne under low N conditions, the length of the primary root axis was longer with defoliation than in an undefoliated control, while the primary root axis diameter decreased. By contrast to F. ovina, the root axes of L. perenne had a more randomly branched topology without defoliation only when supplied with high N. • The greatest plasticity in branching caused by defoliation was observed under high N for L. perenne and under low N for F. ovina. Although grass root axis topology has, in general, a herringbone in structure, the nodal root system can alter root axis structure in response to defoliation.

This paper outlines the construction of a White Paper on Geoethics in Forensic Geology. It focuses on forensic geology, although it also relates to the wider sphere of the forensic geosciences. Forensic geology is rapidly evolving to provide assistance in police investigations and in criminal and civil courts with providing scientific advice and evidence, but there also should be associated clear guidelines to benefit both the practitioner and the justice system. Examples of where forensic geology delivers to society in a vital way are required and also where potential malpractice could happen. The paper discusses where forensic geology should pursue social justice in compliance with current legal systems. In order to achieve this goal, it outlines the main areas that we suggest should be developed within the discipline: the competence of the scientist in forensic geology; the creation of best practice guidelines; the establishing of clear duties of the expert in forensic geology; and consideration of ethical aspects in forensic geological activities and ethical aspects in communicating geoscience evidence. When developing geoethics within forensic geology, the following practices were identified as of prime importance: improved standardization of methods; the use of appropriate methods and/or combination of complementary methods; greater clarity of approach used for the location of areas of interest; collection and recovery of evidence; scene examination and sample collection evaluation of data; construction and appropriate use of databases, background information, documentation, cartography and communication of forensic data; and summary of evidence and acknowledgement and consideration of uncertainty and bias. Honesty, integrity, respect, transparency, competence and reliability are vital for the forensic geoscientist to adhere to. Raising the ethical profile of the forensic geoscience profession aims to pave the way for forensic geoscientists to be empowered now and into the future to serve society: acting responsibly and adopting effective ethical codes is vitally important for a safe society. This paper highlights the necessity to hold urgent discussions on the ethical and social implications of forensic geology and their potential repercussions on societal justice. Forensic geology is a very useful tool, but like any other tool in human hands, it presupposes responsibility in its application. Professionalism and honesty in forensic geology are fundamental to assure the public that geoscientists involved have the highest scientific respectability, social credibility and community respect for their role to help pursue judicial truth. The aim of this draft White Paper is to stimulate an open and informed debate on geoethics.

The Scottish Landscape Alliance is a grouping of over 65 organisations with a common interest in raising awareness of the importance of Scotland’s landscapes to climate resilience and biodiversity, our economic performance and public health and wellbeing and, in doing this, gain public and political support for the better care of Scotland’s landscape and places to maximise future benefits. Its vision is a Scotland where the benefits of landscape are recognised, and where landscape is designed and cared for to strengthen its role in delivering Scotland’s national outcomes, the UN Sustainable Development Goals[i] and the principles of the European Landscape Convention [ii] This presentation (Landscape, Land Use and Economy) accompanies the launch, on 20th October 2020, of a Position Statement[iii]describingthe issues, and makingrecommendations, regarding Scotland's landscapeswith respect to land use and the economy. The Position Statement is accessible at:https://scotlandslandscapealliance.org/position-statement-on-landscape-land-use-and-the-economy-28-09-2020/ References: [i] The 17 UN Sustainable Development Goals were adopted by all United Nations Member States in 2015 as a universal call to action to end poverty, protect the planet and ensure that all people enjoy peace and prosperity by 2030. https://www.undp.org/content/undp/en/home/sustainable-development-goals.html. [ii] The European Landscape Convention (ELC) is the first international treaty dedicated to the protection, management and planning of all landscapes in Europe. Signed by the UK government in 2006 and introduced in March 2007, the ELC provides a people-centred way to reconcile management of the environment with the social and economic challenges of the future, and aims to help people reconnect with place. The ELC covers land and water (inland and seas), and natural, rural, urban and peri-urban landscapes. It includes every-day or degraded landscapes as well as those that might be considered outstanding. https://www.landscapeinstitute.org/policy/13732-2/. [iii]Miller, D.R.; Thomson, J.; Barron, S.; Bate, B.; Brooks, S.; Campbell, G.; Crofts, R.; Dawson, L.A.; Hawkins, M.; Hearns, D.; Hughes. R.; McFarlane, A.; Pretence, P.; Rankine, D.; Thomson, L.; Wade, R.; Younie, S.; Saunders, G.(2020)Position statement on landscape, land use and economy, September 2020., Scotland's Alliance - Working Group 3 Report, September 2020, 17pp.

Most of the forensic comparison of soils has focused on the clay and silt fractions at the expense of the coarser particles. This study aimed to test the potential of elemental and physical analyses in the sand fraction from subtropical soils to discriminate samples collected in areas under different parent material (claystone and marble) and in areas with the same parent material at a simulated crime scene. Scanning electron microscopy coupled to an energy dispersive X-ray spectrometer (SEM-EDS) analysis was used on the finer sized sand particles (0.05-0.25 mm). X-ray diffraction (XRD) and particle size distribution (PSD) analyses were performed on the whole sand fraction. These methods did not provide clear discrimination of the sand of the soils sampled in the subtropical environment. This can be explained by the large homogenization observed in the sand fraction related to its chemical (EDS), physical (particle size distribution), morphological (SEM), and mineralogical (XRD). Under tropical and subtropical conditions, the chemical weathering processes dissolve most of the primary minerals, such as the feldspars, biotite, and Fe-bearing particles, and concentrates quartz in the sand fraction. In these environments, we recommend the prioritization of the finer soil fractions for forensic studies, both inorganic and organic.

Our global food system is under immense pressure. Feeding a growing human population well while simultaneously delivering required climate, biodiversity and other key outcomes arguably represents the biggest challenge of our civilization in the twenty-first century. Here we discuss this growing challenge in the context of Scotland, its progress to date, its new target of “net zero” greenhouse gas emissions by 2045, and its potential to be an exemplar for well-integrated land use policy that delivers on multiple aims. We highlight the role of research in informing rural policy and landowner actions and stress the importance of social science in helping to ensure a sustainable net zero transition that takes full account of socioeconomic contexts and avoids the big potential pitfalls of ignoring local contexts.

In the context of the forensic sciences, geochemical data can give important information as to the sample origin or help to refine and narrow the probable origin. The current study employs ...

We studied the changes in concentration and relative abundance of human-derived fatty acids (FAs) in soil over a period of one year. The study is based on analysis of soil underneath a human body that lay on the soil surface for 18 days before it was discovered. Soil samples were taken when the body was removed, and also 358 days later. Large amounts of the total FA concentration at the start of the measurement period were still present one year after the removal of the body. The FA profile suggested that extensive saturated FA reduction occurred during the first 18 days after deposition. 10-Hydroxystearic acid and FA salts, which are characteristic of adipocere, were abundant in all soil samples from beneath the body. Myristic acid and 10-hydroxystearic acid concentration declined over time. It was therefore concluded that FAs, still detectable one year after the temporal deposition of a body, can serve as an important indicator of human decomposition fluid in soil. Furthermore, the study suggests that in-situ processes such as the formation of adipocere promoted the preservation of human-derived FAs over the period of the study.

Forensic soil science is now an accepted discipline in many nations worldwide such as the UK, the Netherlands, Germany, Australia, Russia, Italy, Japan and the USA. Other nations are adopting the approach suitable for their own types of soils, crimes and land cover. There are a range of methods which can be used, with new methods being researched, developed and tested all the time. This chapter will not discuss the specific analytical methods used but instead outlines suggested strategies for examination and analysis and the presentation and communication of these results in court. The methods adopted for analysis in case work will often depend on the case in question, the examiner, the soil type and the individual country and legal system involved. Forensic soil science application can in general be divided into two main areas: (1) for intelligence gathering, such as providing information on search and narrowing down areas of interest, or in crime reconstruction and (2) for trace evidence comparison, evidence provision, evaluation of data and presentation in court. As the area of forensic soil science is relatively well established, this chapter concentrates on legal aspects of the use, acceptance and application of new methods, particularly acceptance and admissibility in court.

Soils are a complex mixture with a variety of mineralogical, chemical, biological and physical properties, which can be explored within forensic case work. This study aimed to apply energy-dispersive X-ray fluorescence (EDXRF) and Fourier transform infrared (FTIR) spectroscopic techniques to discriminate soil samples collected in southern Brazil in a forensic context. Four replicates of soil were collected at four sites: two sites from the same parent material (claystone) and two other sites from limestone and granite/gneiss respectively. The physical and chemical (organic and mineral composition) and spectroscopic techniques (EDXRF and FTIR) produced 16 quantitative variables from only 2 g of sample. The main results from a forensic context were the separation of the soils collected from close neighbourhoods developed on the same parent material and the separation of soils collected in the A and B horizons of the same soil profile. The highest degree of similarity in the clustering of samples collected at the same site was 98.6% (B horizon in claystone domain). In addition to the parent material, the effect of organic matter on the chemical and mineralogical characteristics of the A horizon was important in the grouping dynamics of samples. This work demonstrated the potential of spectroscopic techniques in a forensic context.

Degradation of soil resources is a serious global environmental issue and it may be made worse by climate change. It is important that soils should be handled with a careful consideration for sustainable agricultural production using environmental friendly techniques. The use of the biosurfactants and also biosurfactants producing microorganisms to improve soil health and to bioremediate polluted soils is an emerging approach. This review is primarily focused on the use of biosurfactants produced by microorganisms and their application to restore soil health and improve agricultural production in a sustainable way. As a perspective of this review, it is proposed that future studies should explain how and when these approaches can be feasible in terms of production costs and availability of these compounds. To the best of our knowledge, this is the first review highlighting the potential applicability of biosurfactants and biosurfactants producing microorganisms for sustainable soil management.

The potential dependence of virus populations on soil types was examined by electron microscopy, and the total abundance of virus particles in four soil types was similar to that previously observed in soil samples. The four soil types examined differed in the relative abundances of four morphological groups of viruses. Machair, a unique type of coastal soil in western Scotland and Ireland, differed from the others tested in having a higher proportion of tailed bacteriophages. The other soils examined contained predominantly spherical and thin filamentous virus particles, but the Machair soil had a more even distribution of the virus types. As the first step in looking at differences in populations in detail, virus sequences from Machair and brown earth (agricultural pasture) soils were examined by metagenomic sequencing after enriching for circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) virus genomes. Sequences from the family Microviridae (icosahedral viruses mainly infecting bacteria) of CRESS-DNA viruses were predominant in both soils. Phylogenetic analysis of Microviridae major coat protein sequences from the Machair viruses showed that they spanned most of the diversity of the subfamily Gokushovirinae , whose members mainly infect obligate intracellular parasites. The brown earth soil had a higher proportion of sequences that matched the morphologically similar family Circoviridae in BLAST searches. However, analysis of putative replicase proteins that were similar to those of viruses in the Circoviridae showed that they are a novel clade of Circoviridae -related CRESS-DNA viruses distinct from known Circoviridae genera. Different soils have substantially different taxonomic biodiversities even within ssDNA viruses, which may be driven by physicochemical factors.

Explosives are a common soil contaminant at a range of sites, including explosives manufacturing plants and areas associated with landmine detonations. As many explosives are toxic and may cause adverse environmental effects, a large body of research has targeted the remediation of explosives residues in soil. Studies in this area have largely involved spiking 'pristine' soils using explosives solutions. Here we investigate the fate of explosives present in soils following an actual detonation process and compare this to the fate of explosives spiked into 'pristine' undetonated soils. We also assess the effects of the detonations on the physical properties of the soils. Our scanning electron microscopy analyses reveal that detonations result in newly-fractured planes within the soil aggregates, and novel micro Computed Tomography analyses of the soils reveal, for the first time, the effect of the detonations on the internal architecture of the soils. We demonstrate that detonations cause an increase in soil porosity, and this correlates to an increased rate of TNT transformation and loss within the detonated soils, compared to spiked pristine soils. We propose that this increased TNT transformation is due to an increased bioavailability of the TNT within the now more porous post-detonation soils, making the TNT more easily accessible by soil-borne bacteria for potential biodegradation. This new discovery potentially exposes novel remediation methods for explosive contaminated soils where actual detonation of the soil significantly promotes subsequent TNT degradation. This work also suggests previously unexplored ramifications associated with high energy soil disruption.

Forensic geology has developed in each country dependent on the history, political and social setting, anthropological influences and geology. The aim of this section is to provide a global overview of forensic geology, including the history, developments and future challenges in Africa, Asia, Australia, Canada, Europe, Middle East, Latin America and the Caribbean, Russia and Commonwealth Independent States (CIS), and USA.

Most methods used in the identification of counterfeit whisky have focused on the profiling of volatile organic congeners determined by gas chromatography. We tested the use of total reflection x-ray fluorescence (TXRF) for trace element analysis of whisky and application of the data as a potential tool in the identification of counterfeit samples. Twenty five whiskies that were produced in different regions of Scotland or were blends, 5 counterfeit whiskies, 1 unmatured grain whisky, and 1 matured grain whisky were analysed for 11 elements (P, S, Cl, K, Ca, Mn, Fe, Cu, Zn, Br and Rb). The effect of cold plasma ashing with oxygen on whisky residues evaporated on the TXRF reflector on the instrument performance was investigated. Cold plasma ashing with oxygen reduced beam scatter and improved the limits of detection but was ultimately deemed unnecessary. The element concentration data for whisky obtained by TXRF (after log transformation) was compared with the values obtained by inductively coupled plasma spectroscopy and showed correlation values (R2) ≥ 0.942 for K, Mn and Cu: ≥ 0.800 for Ca, Fe and Rb; and ≥0.535 for P, S and Zn. The range of concentration values for individual elements was variable and principal components analysis of the elemental concentrations partially differentiated the whiskies by region or type but showed clear separation of the counterfeit samples from the other samples. Using the principal component scores of the elemental concentration data, linear discriminant analysis also distinguished the counterfeits from the other samples.

International audience; The ubiquity and transferability of soil makes it a resource for the forensic investigator, as it can provide a link between agents and scenes. However, the information contained in soils, such as chemical compounds, physical particles or biological entities, is seldom used in forensic investigations; due mainly to the associated costs, lack of available expertise, and the lack of soil databases. The microbial DNA in soil is relatively easy to access and analyse, having thus the potential to provide a powerful means for discriminating soil samples or linking them to a common origin. We compared the effectiveness and reliability of multiple methods and genes for bacterial characterisation in the differentiation of soil samples: ribosomal intergenic spacer analysis (RISA), terminal restriction fragment length polymorphism (TRFLP) of the rpoB gene, and five methods using the 16S rRNA gene: phylogenetic microarrays, TRFLP, and high throughput sequencing with Roche 454, Illumina MiSeq and IonTorrent PGM platforms. All these methods were also compared to long-chain hydrocarbons (n-alkanes) and fatty alcohol profiling of the same soil samples. RISA, 16S TRFLP and MiSeq performed best, reliably and significantly discriminating between adjacent, similar soil types. As TRFLP employs the same capillary electrophoresis equipment and procedures used to analyse human DNA, it is readily available for use in most forensic laboratories. TRFLP was optimized for forensic usage in five parameters: choice of primer pair, fluorescent tagging, concentrating DNA after digestion, number of PCR amplifications per sample and number of capillary electrophoresis runs per PCR amplification. This study shows that molecular microbial ecology methodologies are robust in discriminating between soil samples, illustrating their potential usage as an evaluative forensic tool.

International audience; Soil complexity, heterogeneity and transferability make it valuable in forensic investigations to help obtain clues as to the origin of an unknown sample, or to compare samples from a suspect or object with samples collected at a crime scene. In a few countries, soil analysis is used in matters from site verification to estimates of time after death. However, up to date the application or use of soil information in criminal investigations has been limited. In particular, comparing bacterial communities in soil samples could be a useful tool for forensic science. To evaluate the relevance of this approach, a blind test was performed to determine the origin of two questioned samples (one from the mock crime scene and the other from a 50:50 mixture of the crime scene and the alibi site) compared to three control samples (soil samples from the crime scene, from a context site 25 m away from the crime scene and from the alibi site which was the suspect’s home). Two biological methods were used, Ribosomal Intergenic Spacer Analysis (RISA), and 16S rRNA gene sequencing with Illumina Miseq, to evaluate the discriminating power of soil bacterial communities. Both techniques discriminated well between soils from a single source, but a combination of both techniques was necessary to show that the origin was a mixture of soils. This study illustrates the potential of applying microbial ecology methodologies in soil as an evaluative forensic tool.

A system combining a national soils database with a neural network was developed for prediction of source location for soil samples. The neural network was trained to predict environmental characteristics, which can be of crucial importance to investigating officers in a police operation or to environmental agencies attempting to locate the source of a pollutant. When coupled with maps of environmental conditions and a generalized opinion pool approach, the system was used to produce weighted maps of source location. The system was capable of reducing search areas of a sample source to less than 0.1% of the total area.

Interpretation of sedimentary n-alkyl lipid d2H data is complicated by a limited understanding of factors controlling interspecies variation in biomarker 2H/1H composition. To distinguish between the effects of interrelated environmental, physical and biochemical controls on the hydrogen isotope composition of n-alkyl lipids, we conducted linked d2H analyses of soil water, xylem water, leaf water and n-alkanes from a range of C3 and C4 plants growing at a UK saltmarsh (i) across multiple\ud sampling sites, (ii) throughout the 2012 growing season, and (iii) at different times of the day. Soil waters varied isotopically by up to 35& depending on marsh sub-environment, and exhibited site-specific seasonal shifts in d2H up to a maximum of 31 per mil. Maximum interspecies variation in xylem water was 38 per mil, while leaf waters differed seasonally by a maximum of 29 per mil. Leaf wax n-alkane 2H/1H, however, consistently varied by over 100 per mil throughout the 2012 growing season, resulting in an interspecies range in the ewax/leaf water values of -79 per mil to –227 per mil. From the discrepancy in the magnitude of these isotopic differences, we conclude that mechanisms driving variation in the 2H/1H composition of leaf water, including (i) spatial changes in soil water 2H/1H, (ii) temporal changes in soil water 2H/1H, (iii) differences in xylem water 2H/1H, and (iv) differences in leaf water evaporative 2H-enrichment due to varied plant life forms, cannot explain the range of n-alkane d2H values we observed. Results from this study suggests that accurate reconstructions of palaeoclimate regimes from sedimentary n-alkane d2H require further research to constrain those biological mechanisms influencing species-specific differences in 2H/1H fractionation during lipid biosynthesis, in particular where plants have developed biochemical adaptations to water-stressed conditions. Understanding how these mechanisms interact with environmental conditions will be crucial to ensure accurate\ud interpretation of hydrogen isotope signals from the geological record.

In July 2013 the International School Science Fair (ISSF) was hosted by Camborne Science and International Academy, Cornwall, UK. This meeting brings young talented scientists together from around the world to participate in workshops and activities highlighting current scientific developments. As part of ISSF 2013, a workshop on forensic geology was delivered to some of the international participants. This included the preparation of a map to show the mineralogical composition of the soils of the participating schools. The soil mineralogy was determined using automated mineral analysis based on scanning electron microscopy. In addition there were workshops on the recovery of geological trace evidence in a forensic context and the theory and practice of carrying out a geophysical search for hidden items. Data generated as part of this workshop are available to download from the International Union of Geological Sciences, Initiative on Forensic Geology website (http://www.forensicgeologyinternational.com).

This study was carried out to evaluate the potential of using cholesterol and coprostanol, as indicators for the detection of decomposition fluid of buried pigs (S. s. domesticus) in soils. In May 2007, four pig carcasses (~35. kg) were buried in shallow graves (~40. cm depth) at the University of Ontario Institute of Technology in Canada. Two pigs were exhumed after three months (Pig 1, Pig 2) and six months (Pig 3, Pig 4) post burial. Soil samples were collected beneath the pig carcasses (~40. cm depth) and from grave walls (~15-20. cm depth) as well as from a parallel control site. Coprostanol and cholesterol were extracted from soils, purified with solid phase extraction (SPE) and analysed with gas chromatography/mass spectrometry (GC/MS). A significant increase in cholesterol concentrations (p

The use of chemical enhancement techniques on porous substrates, such as fabrics, poses several challenges predominantly due to the occurrence of background staining and diffusion as well as visualization difficulties. A range of readily available chemical and lighting techniques were utilized to enhance footwear impressions made in blood, soil, and urine on dark and patterned fabrics. Footwear impressions were all prepared at a set force using a specifically built footwear rig. In most cases, results demonstrated that fluorescent chemical techniques were required for visualization as nonfluorescent techniques provided little or no contrast with the background. Occasionally, this contrast was improved by oblique lighting. Successful results were obtained for the enhancement of footwear impressions in blood; however, the enhancement of footwear impressions in urine and soil on dark and patterned fabrics was much more limited. The results demonstrate that visualization and fluorescent enhancement on porous substrates such as fabrics is possible.

Many subdisciplines within the Earth Sciences make use of either geophysical instruments to investigate the subsurface environment or use analytical methods to determine the origin, or provenance, of geological materials. These same instruments and analytical methods can be used either directly, or adapted to suit, the acquisition of data that pertain to a wide range of forensic science investigations. Such approaches, as discussed below, are generally not new but, in recent years, there has been a significant resurgence globally in the application of geological and geophysical methods to aid forensic investigations. Traditionally, such methods were used in forensic investigations related to serious criminal cases such as terrorism, murder, abduction and serious sexual assaults, and to a lesser extent in the investigation of cases of fraud and theft. But with increasing concern into the environmental impact of human activity, with the release of contaminants into the atmosphere, hydrosphere and lithosphere, and their potential uptake into the biosphere, there has been an increased amount of environmental legislation. In turn, there has commonly been an increase in the costs associated with the legal discharge or disposal of wastes. Consequently, it is unsurprising that the illegal discharge and disposal of wastes has also increased. Identifying the distribution, impact and source of such waste materials can, in part, be addressed through the application of geological techniques, in much the same way as used traditionally, for example, in the investigation of murder cases. The diversification of the use of geological techniques into the investigation of environmental crime will, potentially, significantly increase the range of investigations in which geologists may be asked to assist but will also lead to a new array of research questions to be addressed, hence the need for this Special Publication. In this short introduction, the aim is to place the current work into its broad historical context and summarize the key findings from the papers presented within this volume. The papers within this Special Publication were presented at two separate conferences in 2010: the Third International Soil Forensics Conference held at Long Beach California USA and organized by the SFI Group; and a conference on Environmental and Criminal Forensics, organized by the Forensic Geoscience Group of the Geological Society of London, and held at Burlington House, London, in December 2010.

The carbon isotope composition of terrestrial plants records valuable ecophysiological and palaeoecological information. However, interspecies variability in 13C/12C, at both the bulk and compound-specific (CS) level, requires further exploration across a range of ecosystem types. Here, we present bulk and n-alkane δ13C values, and n-alkane concentrations, from seven plants (C3 and C4) growing in a temperate UK saltmarsh. Inter- and intra-species variation in n-alkane δ13C values among C3 plants ranged from 8‰ (n-C31) to 10‰ (n-C27) across the 2011 and 2012 growing seasons, exceeding variability in bulk tissue (7‰). In contrast, the C4 monocot showed < 2‰ seasonal shifts in bulk and CS values. As a result of the variability in our CS data, we calculate that n-alkane based C3/C4 reconstructions in temperate saltmarshes have a maximum uncertainty of ∼11%. For dicots and succulents, seasonal bulk and CS δ13C trends diverged, while for C3 and C4 monocots, bulk and CS values followed similar temporal patterns. Fractionation between bulk and n-alkane carbon isotope values varied from −4 to −10‰ for C3 plants, and reached −13‰ for the C4 monocot. We explain discrepancies between bulk and n-alkane δ13C values by referring to possible interspecies variation in salinity adaptation, which may influence the partitioning of pyruvate, shifting the isotopic composition of lipid biomarkers. These findings open new avenues for empirical studies to further understand the metabolic processes fractionating carbon during the synthesis of n-alkanes, enhancing interpretation of the biomarker signal from the geological record.

Influenza A virus (IAV) is economically important in pig production and has broad public health implications. In Europe, active IAV surveillance includes demonstration of antigen in nasal swabs and/or demonstration of antibodies in serum (SER) samples; however, collecting appropriate numbers of individual pig samples can be costly and labour‐intensive. The objective of this study was to compare the probability of detecting IAV antibody positive populations using SER versus oral fluid (OF) samples. Paired pen samples, one OF and 5–14 SER samples, were collected cross‐sectional or longitudinally. A commercial nucleoprotein (NP)‐based blocking ELISA was used to test 244 OF and 1004 SER samples from 123 pens each containing 20–540 pigs located in 27 UK herds. Overall, the IAV antibody detection rate was higher in SER samples compared to OFs under the study conditions. Pig age had a significant effect on the probability of detecting positive pens. For 3–9‐week‐old pigs the probability of detecting IAV antibody positive samples in a pen with 95% confidence intervals was 40% (23–60) for OF and 61% (0.37–0.80) for SER (P = 0.04), for 10–14‐week‐old pigs it was 19% (8–40) for OF and 93% (0.71–0.99) for SER (P

Over the past 20 years, the discipline of forensic archaeology has established itself in the United Kingdom as a primary method of detection for buried human remains. This has been achieved predominantly through the recognition of specific variations, patterning and disturbances in landscape, geological, botanical and ground signatures. These interpretations have subsequently been greatly enhanced by an increasingly sophisticated understanding, adoption and utilisation of geophysical search equipment and search techniques. In addition, the application of traditional archaeological excavation methodologies to criminal investigations that involve buried human remains can be seen to have become an important milestone in optimising an investigating team’s ability to elucidate and extract evidence from the grave and burial environment (Hunter et al. 1995; Groen et al. 2015).

This study investigates the enhancement of footwear impressions prepared with soils from different locations on a variety of fabric surfaces with different morphology. Preliminary experiments using seventeen techniques were carried out and the best responding reagents were evaluated further. Results indicated that the soils investigated (a cross-section of soils from Scotland) are more likely to respond to reagents that target iron ions rather than calcium, aluminium or phosphorus ions. Furthermore, the concentration of iron and soil pH did not appear to have an effect on the performance of the enhancement techniques. For the techniques tested, colour enhancement was observed on all light coloured substrates while enhancement on dark coloured fabrics, denim and leatherette was limited due to poor contrast with the background. Of the chemical enhancement reagents tested, 2,2'-dipyridil was a suitable replacement for the more common enhancement technique using potassium thiocyanate. The main advantages are the use of less toxic and flammable solvents and improved clarity and sharpness of the enhanced impression. The surface morphology of the fabrics did not have a significant effect on the enhancement ability of the reagents apart from a slight tendency for diffusion to occur on less porous fabrics such as polyester and nylon/lycra blends.