1. Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
- Author
-
Javier Bezos, Lucas Domínguez, Beatriz Romero, Nacho Aguilo, Irene Tomé, Mercedes Domínguez, Carlos Martin, Álvaro Roy, Eugenia Puentes, Esteban M. Rodríguez, María Ángeles Risalde, Victor Lorente-Leal, Christian Gortázar, Lucía de Juan, José Antonio Infantes-Lorenzo, Ministerio de Economía, Industria y Competitividad (España), Ministerio de Economía y Competitividad (España), European Commission, [Roy,A, Puentes,E, Rodríguez,E] BIOFABRI S.L., Porriño, Pontevedra, Spain. [Roy,A, Tomé,I, Romero,B, Lorente-Leal,V, Infantes-Lorenzo,JA, de Juan,L, Domínguez,L, Bezos,J] VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, Spain. [Domínguez,M] Servicio de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Investigación Carlos III, Majadahonda, Madrid, Spain. [Martín,C, Aguiló,N] Grupo de Genética de Micobacterias, Departamento de Microbiología y Medicina Preventiva, Facultad de Medicina, Universidad de Zaragoza, Zaragoza, Spain. [Martín,C, Aguiló,N] CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain. [Martín,C] Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain. [de Juan,L, Bezos,J] Dpto. de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain. [Risalde,MA] Dpto. de Anatomía y Anatomía Patológica Comparadas, Universidad de Córdoba, Córdoba, Spain. [Risalde,MA] Infectious Diseases Unit, Instituto Maimonides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofía de Córdoba, Universidad de Córdoba, Córdoba, Spain. [Gortázar,C] SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain., and This work was funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO, Grant Number: IPT-2012-0327-090000). AR is the recipient of an Industrial Doctorate contract (DI15-08110) funded by the MINECO and the European Social Fund. JB is the recipient of a Juan de la Cierva Incorporation Research contract (IJCI-2015-24805) funded by MINECO.
- Subjects
Vacuna BCG ,[SDV]Life Sciences [q-bio] ,0403 veterinary science ,Chemicals and Drugs::Complex Mixtures::Biological Products::Vaccines::Vaccines, Attenuated [Medical Subject Headings] ,Immunogenicity, Vaccine ,Mycoplasma ,Vacunas bacterianas ,Organisms::Eukaryota::Animals [Medical Subject Headings] ,Tuberculosis Vaccines ,Natural transmission ,0303 health sciences ,Mycobacterium bovis ,Cabra ,Immunity, Cellular ,lcsh:Veterinary medicine ,Attenuated vaccine ,Infecciones por mycobacterium ,biology ,Immunogenicity ,Phenomena and Processes::Immune System Phenomena::Immunity::Adaptive Immunity::Immunity, Cellular [Medical Subject Headings] ,Goats ,Chemicals and Drugs::Complex Mixtures::Biological Products::Vaccines::Bacterial Vaccines::Tuberculosis Vaccines [Medical Subject Headings] ,04 agricultural and veterinary sciences ,3. Good health ,Bacterial vaccine ,Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Mycobacterium tuberculosis [Medical Subject Headings] ,BCG Vaccine ,Organisms::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma [Medical Subject Headings] ,Bacterial vaccines ,Research Article ,Tuberculosis ,040301 veterinary sciences ,Transmisión natural ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Artiodactyla::Ruminants::Goats [Medical Subject Headings] ,Chemicals and Drugs::Complex Mixtures::Biological Products::Vaccines::Bacterial Vaccines::Tuberculosis Vaccines::BCG Vaccine [Medical Subject Headings] ,Vaccines, Attenuated ,Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis [Medical Subject Headings] ,Mycobacterium tuberculosis ,03 medical and health sciences ,Antigen ,medicine ,Mycobacterium infections ,Phenomena and Processes::Immune System Phenomena::Immunity::Adaptive Immunity::Immunity, Humoral [Medical Subject Headings] ,Humans ,Animals ,Diseases::Animal Diseases::Goat Diseases [Medical Subject Headings] ,030304 developmental biology ,Goat Diseases ,General Veterinary ,medicine.disease ,biology.organism_classification ,Immunity, Humoral ,Immunology ,lcsh:SF600-1100 ,BCG vaccine - Abstract
Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observed in the control group until challenge. All animals showed lesions consistent with TB at the end of the study. Goats that received either vaccine showed significantly lower scores for pulmonary lymph nodes and total lesions than unvaccinated controls. Both MTBVAC and BCG vaccines proved to be immunogenic and effective in reducing severity of TB pathology caused by M. caprae. Our model system of natural TB transmission may be useful for evaluating and optimizing vaccines., This work was funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO, Grant Number: IPT-2012-0327-090000). AR is the recipient of an Industrial Doctorate contract (DI15-08110) funded by the MINECO and the European Social Fund. JB is the recipient of a Juan de la Cierva Incorporation Research contract (IJCI-2015-24805) funded by MINECO.
- Published
- 2019
- Full Text
- View/download PDF