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15 results on '"Lopomo, N. F."'

1. A Preliminary Study on Aerosol Jet-Printed Stretchable Dry Electrode for Electromyography

Author

Borghetti, M., Fapanni, Tiziano, Lopomo, N. F., Sardini, E., Serpelloni, M., Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Saponara, Sergio, editor, and De Gloria, Alessandro, editor

Published
2021
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2. Using LPWAN Connectivity for Elderly Activity Monitoring in Smartcity Scenarios

Author

Fernandes Carvalho, D., Ferrari, P., Sisinni, E., Bellitti, P., Lopomo, N. F., Serpelloni, M., Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Saponara, Sergio, editor, and De Gloria, Alessandro, editor

Published
2020
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7. 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Author

Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, N, Sardini, E, Borsani, E, Rodella, L, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M, Russo, D, Re F., Sartore L., Moulisova V., Cantini M., Almici C., Bianchetti A., Chinello C., Dey K., Agnelli S., Manferdini C., Bernardi S., Lopomo N. F., Sardini E., Borsani E., Rodella L. F., Savoldi F., Paganelli C., Guizzi P., Lisignoli G., Magni F., Salmeron-Sanchez M., Russo D., Re, F, Sartore, L, Moulisova, V, Cantini, M, Almici, C, Bianchetti, A, Chinello, C, Dey, K, Agnelli, S, Manferdini, C, Bernardi, S, Lopomo, N, Sardini, E, Borsani, E, Rodella, L, Savoldi, F, Paganelli, C, Guizzi, P, Lisignoli, G, Magni, F, Salmeron-Sanchez, M, Russo, D, Re F., Sartore L., Moulisova V., Cantini M., Almici C., Bianchetti A., Chinello C., Dey K., Agnelli S., Manferdini C., Bernardi S., Lopomo N. F., Sardini E., Borsani E., Rodella L. F., Savoldi F., Paganelli C., Guizzi P., Lisignoli G., Magni F., Salmeron-Sanchez M., and Russo D.

Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Published
2019

8. New models of care and multidimensional solutions for oncological patients in the post-acute SARS-COV-2 period: a “Second Phase” also for cancer patients.

Author

CONSOLI, F., CRISTINI, C., GUARINONI, M. G., LEVAGGI, R., LOPOMO, N. F., MAROLDI, R., MELCHIORI, M., MOTTA, P. C., SARDINI, E., BERRUTI, A., and BOSSI, P.

Abstract

In Italy, SARS-CoV-2 outbreak registered a high transmission and disease rates. During the acute phase, oncologists provided to re-organize services and prioritize treatments, in order to limit viral spread and to protect cancer patients. The progressive reduction of the number of infections has prompted Italian government to gradually loosen the national confinement measures and to start the “Second phase” of measures to contain the pandemic. The issue on how to organize cancer care during this postacute SARS-CoV-2 phase appears crucial and a reassessment of healthcare services is needed requiring new models of care for oncological patients. In order to address major challenges in cancer setting during post-acute SARS-CoV-2 phase, this work offers multidimensional solutions aimed to provide a new way to take care of cancer patients. [ABSTRACT FROM AUTHOR]

Published
2020

11. How cartilage status can be related to joint loads in anterior cruciate ligament reconstruction: a preliminary analysis including MRI t2 mapping and joint biomechanics

Author

Cassiolas, G., Berni, M., Marchiori, G., Bontempi, M., LAURA BRAGONZONI, Valente, G., Grassi, A., Zaffagnini, S., Visani, A., Lopomo, N. F., Cassiolas, G., Berni, M., Marchiori, G., Bontempi, M., Bragonzoni, L., Valente, G., Grassi, A., Zaffagnini, S., Visani, A., and Lopomo, N.F.

Subjects
musculoskeletal diseases, Cartilage, Articular, Cancer Research, Anterior Cruciate Ligament Reconstruction, Knee Joint, Physiology, Endocrinology, Diabetes and Metabolism, Anterior Cruciate Ligament Injuries, Immunology, musculoskeletal system, Magnetic Resonance Imaging, Biomechanical Phenomena, Endocrinology, Oncology, Physiology (medical), Immunology and Allergy, Humans, human activities
Abstract

The knee is the largest and most complex joint in the human body. Traumatic events, such as anterior cruciate ligament (ACL) tear, can lead to an alteration of joint tissues homeostasis. Literature reports an evident correlation between abnormal joint biomechanics and the status of articular tissues. These alterations, due to a sub-optimal ACL reconstruction, may result in an increasing risk of developing degenerative pathologies, such as osteoarthritis. Thus, the identification of the optimal surgical technique is a highly demanding issue in ACL reconstruction. The aim of this study was to analyze the correlation between joint cartilage conditions and knee biomechanics in ACL reconstructions, by integrating MRI T2 mapping investigations, radiostereophotogrammetry-based gait analysis and subject-specific musculoskeletal modelling.

12. Carbon on poly(ε-caprolactone) (PCL) ink-jet printed sensor for monitoring cell cultures of myoblasts

Author

Mariagrazia Marziano, Sarah Tonello, Maurizio Memo, Maurizio Toselli, Michela Borghetti, Marialaura Serzanti, Emilio Sardini, Maria Letizia Focarete, Stefano Pandini, Daniela Uberti, Patrizia Dell'Era, Mauro Serpelloni, Chiara Gualandi, Andrea Merlettini, Massimo Messori, Nicola Lopomo, Marziano, M., Tonello, S., Serzanti, M., Borghetti, M., Lopomo, N. F., Serpelloni, M., Pandini, S., Merlettini, A., Gualandi, C., Focarete, M. L., Messori, M., Toselli, M., Uberti, D., Memo, M., Dell’Era, P., and Sardini, E.

Subjects
Scaffold, Materials science, Biocompatibility, Biomedical Engineering, Bioengineering, 02 engineering and technology, Substrate (printing), 010402 general chemistry, 01 natural sciences, Cell monitoring, Electrochemical Cell-substrate Impedance Spectroscopy (ECIS), Ink-jet printed sensors, chemistry.chemical_compound, Composite material, Cell adhesion, Inkwell, Ink-jet printed sensor, Adhesion, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Dielectric spectroscopy, chemistry, 0210 nano-technology, Caprolactone, Biomedical engineering
Abstract

Nowadays techniques for sensitive non-invasive, real-time monitoring of cell differentiation and maturation are highly demanded. In light of this, the development of electrochemical printed sensors impedance-based could represent a promising tool. In the present work, we developed 2D ink-jet printed sensors for myoblasts adhesion monitoring, using carbon-based ink on a substrate consisting in non-woven electrospun mats made in crosslinked poly(ε-caprolactone) (PCL). First of all, sensors printability was optimized and the biocompatibility tested. In order to determine the possibility to employ the prepared systems as scaffolds for dynamic cellular cultures, the mechanical response of the PCL scaffold was evaluated through the application of cyclic deformation tests. After that, electrical characterization of ink and substrate was performed, followed by electrochemical impedance-based measurements to evaluate myoblasts adhesion. Biocompatibility assessment showed good results for both carbon and PCL. Mechanical tests findings suggested that a training of 50 cycles and a proper value of strain should be applied before the cell seeding, in order to ensure a subsequent controlled strain amplitude. The sensorized scaffold allowed us to correlate cell adhesion with an increase of impedance module, in agreement with biocompatibility testing. Thus, this first preliminary testing suggested that this non-invasive impedance spectroscopy-based measurement system can be used for sensitive monitoring of cells adhesion, in static and moreover, as suggested from mechanical characterization, in dynamic conditions.

Published
2017

13. Monitoring Caco-2 to enterocyte-like cells differentiation by means of electric impedance analysis on printed sensors.

Author

Marziano M, Tonello S, Cantù E, Abate G, Vezzoli M, Rungratanawanich W, Serpelloni M, Lopomo NF, Memo M, Sardini E, and Uberti D

Subjects
Caco-2 Cells, Cell Proliferation, Electrodes, Humans, Cell Differentiation, Electric Impedance, Electrochemical Techniques, Printing, Three-Dimensional
Abstract

Background: Colorectal adenocarcinoma cells (Caco-2) are a widely used model of intestinal barrier to study cancer development, toxicological assessments, absorption and metabolism in food science or drug discovery. Caco-2 spontaneously differentiate into a monolayer expressing several specific characteristics, typically showed by mature enterocytes. For in vitro experiments, it is crucial to identify non-invasive and non-destructive techniques able to evaluate the integrity and differentiation of the cells monolayer. Thus, we aimed to assess these properties by analyzing electrical impedance measurements., Methods: Caco-2 cells were differentiated for 21 days. The monolayer integrity and differentiation were primarily evaluated by means of morphological, biochemical and molecular data. Impedance measurements in a range of frequencies from 400 Hz to 50 kHz were performed using a dedicated set up, including customized Aerosol Jet Printed carbon-based sensors., Results: The trends of RI observed at three different frequencies were able to describe cell growth and differentiation. In order to evaluate which frequencies better correlate with cell differentiation, Principal Component Analysis have been employed and the concordance analysis between RI magnitude and morphological, biochemical and molecular data, highlighted 40 kHz as the optimal frequency to assess Caco-2 cells differentiation process., Conclusion: We demonstrated the feasibility and reliability of applying impedance-based measurements not only to provide information about the monolayer status, but also for cell differentiation monitoring., General Significance: This study underlined the possibility to use a dedicated sensor to assess the integrity and differentiation of Caco-2 monolayer, as a reliable non-destructive alternative to conventional approaches., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Published
2019
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14. How cartilage status can be related to joint loads in anterior cruciate ligament reconstruction: a preliminary analysis including MRI t2 mapping and joint biomechanics.

Author

Cassiolas G, Berni M, Marchiori G, Bontempi M, Bragonzoni L, Valente G, Grassi A, Zaffagnini S, Visani A, and Lopomo NF

Subjects
Biomechanical Phenomena, Humans, Knee Joint diagnostic imaging, Magnetic Resonance Imaging, Anterior Cruciate Ligament Injuries diagnostic imaging, Anterior Cruciate Ligament Reconstruction, Cartilage, Articular diagnostic imaging
Abstract

The knee is the largest and most complex joint in the human body. Traumatic events, such as anterior cruciate ligament (ACL) tear, can lead to an alteration of joint tissues homeostasis. Literature reports an evident correlation between abnormal joint biomechanics and the status of articular tissues. These alterations, due to a sub-optimal ACL reconstruction, may result in an increasing risk of developing degenerative pathologies, such as osteoarthritis. Thus, the identification of the optimal surgical technique is a highly demanding issue in ACL reconstruction. The aim of this study was to analyze the correlation between joint cartilage conditions and knee biomechanics in ACL reconstructions, by integrating MRI T2 mapping investigations, radiostereophotogrammetry-based gait analysis and subject-specific musculoskeletal modelling.

Published
2018

15. Effects of working gas pressure on zirconium dioxide thin film prepared by pulsed plasma deposition: roughness, wettability, friction and wear characteristics.

Author

Berni M, Marchiori G, Gambardella A, Boi M, Bianchi M, Russo A, Visani A, Marcacci M, Pavan PG, and Lopomo NF

Subjects
Friction, Humans, Plasma Gases, Polyethylenes, Pressure, Surface Properties, Wettability, Materials Testing, Prostheses and Implants, Zirconium analysis
Abstract

In joint arthroplasty one of the main issues related to the failure of prosthetic implants is due to the wear of the ultra-high molecular weight polyethylene (UHMWPE) component. Surface treatments and coatings have been recognized as enhancing methods, able to improve the tribological properties of the implants. Therefore, the main objective of this work was to investigate the possibility to fabricate yttria-stabilized zirconia (YSZ) coatings on a metal (AISI 316-L) substrate by means of Pulsed Electron Deposition, in order to improve the tribological behavior of the polymer-metal coupling, by reducing the initial wear of the UHMWPE component. In order to optimize the coating characteristics, the effects of working gas pressure on both its morphological and tribological properties were analyzed. Morphological characterization of the films was evaluated by Atomic Force Microscopy (AFM). Coating wettability was also estimated by contact angle (CA) measurement. Tribological performance (coupling friction and wear of UHMWPE) was evaluated by using a ball-on-disc tribometer during highly-stressing tests in dry and lubricated (i.e. NaCl and serum) conditions; friction and wear were specifically evaluated at the initial sliding distances - to highlight the main effect of coating morphology - and after 100m - where the influence of the intrinsic materials properties prevails. AFM analysis highlighted that the working pressure heavily affected the morphological characteristics of the realized films. The wettability of the coating at the highest and lowest deposition pressures (CA ~ 60°, closed to substrate value) decreased for intermediate pressures, reaching a maximum CA of ~ 90°. Regarding tribological tests, a strong correlation was found in the initial steps between friction coefficient and wettability, which decreased as the distance increased. Concerning UHMWPE wear associated to coated counterpart, at 100m a reduction rate of about 7% in dry, 12% in NaCl and 5% in presence of serum was obtained compared to the uncoated counterpart. Differently from what highlighted for friction, no correlation was found between wear rate and morphological parameters. These findings, in agreement with literature, underlined the effect of the deposition pressure on the morphological properties, but suggested that physical characteristics are influenced too. Further research on the deposition process will be required in order to improve the tribological performance of the coating at long distances, addressing - above all - orthopedic applications., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Published
2017
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