498 results on '"Lopes FL"'
Search Results
2. Developmental Genome-Wide DNA Methylation Asymmetry Between Mouse Placenta and Embryo
- Author
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Legault, LM, primary, Doiron, K, additional, Lemieux, A, additional, Caron, M, additional, Chan, D, additional, Lopes, FL, additional, Bourque, G, additional, Sinnett, D, additional, and McGraw, S, additional
- Published
- 2019
- Full Text
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3. Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation
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Motheo, TF, primary, Arnold, DR, additional, Padilha-Nakaghi, LC, additional, Pires-Buttler, EA, additional, Alves, AE, additional, Apparicio, M, additional, Vicente, WRR, additional, and Lopes, FL, additional
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- 2016
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4. Reduction of heart rate variability in middle-aged individuals and the effect of strength training
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Lopes,FL, Pereira,FM, Reboredo,MM, Castro,TM, Vianna,JM, Novo Jr,JM, and Silva,LP
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physical exercise ,sistema nervoso autônomo ,autonomic nervous system ,exercício físico ,heart rate ,freqüência cardíaca ,meia-idade ,middle age - Abstract
OBJETIVO: Investigar a modulação autonômica exercida sobre o nodo sinusal, por meio da análise da variabilidade da freqüência cardíaca (VFC), em indivíduos jovens e de meia-idade (MI), bem como os efeitos de um programa de treinamento de força resistência sobre tal modulação nos indivíduos de meia-idade. MÉTODO: Trinta e dois homens sadios, sedentários e não-tabagistas, sendo 10 jovens (22,2 ± 1,5 anos) e 22 de MI (49,3 ± 5,3 anos), foram submetidos à aquisição do sinal de eletrocardiograma para análise da VFC no domínio do tempo. Os indivíduos de MI foram divididos em dois grupos: experimental (n= 12) e controle (n= 10). Os indivíduos do grupo experimental foram inseridos em um programa de treinamento de força com duração de três meses. A análise dos dados foi realizada por meio dos testes de Wilcoxon e Mann-Whitney (p< 0,05). RESULTADOS: O grupo MI apresentou redução significativa, em comparação ao jovem, de todas as variáveis utilizadas para a investigação da VFC (SDNN= 33,4 vs. 49,7ms; RMSSD= 29,9 vs. 49,5ms; pNN50= 6,5 vs. 27%). O treinamento promoveu aumento significativo da força e resistência muscular em todos os grupamentos musculares e aumento não significativo das variáveis SDNN (33,4 vs. 37,6ms), RMSSD (30,2 vs. 31,3ms) e pNN50 (7,5 vs. 11,4%). CONCLUSÕES: Os achados deste estudo confirmam que o aumento da idade provoca alteração na modulação autonômica exercida sobre o nodo sinusal, retratada por uma diminuição da VFC em indivíduos de MI, que não foi modificada de maneira significativa pelo tipo de treinamento físico estudado. OBJECTIVE: To investigate autonomic modulation of the sinus node, by analyzing heart rate variability (HRV) among young and middle-aged individuals, and to assess the effect of an endurance strength training program on this modulation among middle-aged individuals. METHOD: Thirty-two healthy nonsmoking men with sedentary lifestyles, of whom 10 were young (22.2 ± 1.5 years) and 22 were middle-aged (49.3 ± 5.3 years), underwent electrocardiogram signal acquisition for time-domain HRV analysis. The middle-aged individuals were divided into two groups: experimental (n= 12) and control (n= 10). The individuals in the experimental group were enrolled in a strength training program lasting three months. The data analysis was carried out using the Wilcoxon and Mann-Whitney tests (p< 0.05). RESULTS: The middle-aged group presented significant reductions (in relation to the young group) for all the variables used in investigating HRV (SDNN= 33.4 vs. 49.7 ms; RMSSD= 29.9 vs. 49.5 ms; pNN50= 6.5 vs. 27%). The training caused a significant increase in muscle strength and endurance for all muscular groups and non-significant increases in the variables SDNN (33.4 vs. 37.6 ms), RMSSD (30.2 vs. 31.3 ms) and pNN50 (7.5 vs. 11.4%). CONCLUSIONS: The findings from this study confirm that increased age causes alteration to the autonomic modulation of the sinus node, as demonstrated by reduced HRV in middle-aged individuals, which was not significantly modified by the type of physical training studied.
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- 2007
5. Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation.
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Motheo, TF, Arnold, DR, Padilha‐Nakaghi, LC, Pires‐Buttler, EA, Alves, AE, Apparicio, M, Vicente, WRR, and Lopes, FL
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LYSINE ,HISTONE acetylation ,OVUM ,CHROMATIN ,GENE expression ,IMMUNOFLUORESCENCE - Abstract
Contents Post-translational modifications of histones, such as acetylation, are involved in regulating chromatin remodelling and gene expression. Proper in vitro maturation ( IVM) of canine oocytes, for many reasons, is up to now inefficient. This study aimed to evaluate the post-translational histone H4 acetylation at lysine 5 (H4K5) in immature and post- IVM canine oocytes. Oocyte nuclear stage was assessed using Hoechst 33342 staining. Acetylation patterns were determined by indirect immunofluorescence staining of immature and post- IVM oocytes, using an antibody against the acetylated lysine 5 residue on histone 4 (H4K5ac). The experiment was repeated four times, with a total of 7-17 oocytes evaluated per stage. Immunofluorescence signal was quantified using the NIH imagej software. Data were expressed as a percentage of the average fluorescence intensity of the specific antibody over the intensity of DNA, as determined by Hoescht staining. H4K5ac displayed a significantly higher acetylated pattern in immature oocytes (0.97 ± 0.08) when compared to post- IVM oocytes at different nuclear stages. There was a decrease in the fluorescence level of the matured oocytes with the progression of meiosis ( GVBD: 0.47 ± 0.06 and MI/ MII: 0.35 ± 0.04). Similarly to other domestic species, we hypothesized that post-translational modification of histone acetylation takes place during meiosis of in vitro matured canine oocytes. However, it remains to be investigated whether these changes occur during in vivo maturation. [ABSTRACT FROM AUTHOR]
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- 2017
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6. In vitro Culture of Bovine Embryos in Murine ES Cell Conditioned Media Negatively Affects Expression of Pluripotency‐Related Markers OCT4, SOX2 and SSEA1
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Oliveira, CS, primary, de Souza, MM, additional, Saraiva, NZ, additional, Tetzner, TAD, additional, Lima, MR, additional, Lopes, FL, additional, and Garcia, JM, additional
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- 2011
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7. Redução da variabilidade da freqüência cardíaca em indivíduos de meia-idade e o efeito do treinamento de força
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Lopes, FL, primary, Pereira, FM, additional, Reboredo, MM, additional, Castro, TM, additional, Vianna, JM, additional, Novo Jr, JM, additional, and Silva, LP, additional
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- 2007
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8. Cutting-edge application of ultrasound elastography and superb microvascular imaging in radiofrequency ablation of uterine fibroids
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Coutinho Cristiana, Werner Heron, Lopes Flávia Paiva, Zelaquett Michel, Marchiori Edson, and Araujo Edward
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Medicine (General) ,R5-920 ,Medical technology ,R855-855.5 - Published
- 2021
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9. In vitro Culture of Bovine Embryos in Murine ES Cell Conditioned Media Negatively Affects Expression of Pluripotency-Related Markers OCT4, SOX2 and SSEA1.
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Oliveira, CS, de Souza, MM, Saraiva, NZ, Tetzner, TAD, Lima, MR, Lopes, FL, and Garcia, JM
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EMBRYONIC stem cells ,GENE expression ,BIOMARKERS ,CELL culture ,CELL differentiation ,CELLULAR mechanics ,GENETIC regulation - Abstract
Contents Despite extensive efforts, establishment of bovine embryonic stem (ES) cell lines has not been successful. We hypothesized that culture conditions for in vitro-produced (IVP) embryos, the most used source of inner cell mass (ICM) to obtain ES cells, might affect their undifferentiated state. Therefore, the aim of this work was to improve pluripotency of IVP blastocysts to produce suitable ICM for further culturing. We tested KSR and foetal calf serum (FCS) supplements in SOF medium and ES cell conditioned medium (CM) on IVC (groups: KSR, KSR CM, FCS and FCS CM). Cleavage and blastocyst rates were similar between all groups. Also, embryonic quality, assessed by apoptosis rates (TUNEL assay), total cell number and ICM percentage did not differ between experimental groups. However, expression of pluripotency-related markers was affected. We detected down-regulation of OCT3/4, SOX2 and SSEA1 in ICM of FCS CM blastocysts (p < 0.05). SOX2 gene expression revealed lower levels (p < 0.05) on KSR CM blastocysts and a remarkable variation in SOX2 mRNA levels on FCS-supplemented blastocysts. In conclusion, pluripotency-related markers tend to decrease after supplementation with ES cell CM, suggesting different mechanisms regulating mouse and bovine pluripotency. KSR supplementation did not differ from FCS, but FCS replacement by KSR may produce blastocysts with stable SOX2 gene expression levels. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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10. Symbolic violence experienced by men who have sex with men in the primary health service in Fortaleza, Ceará, Brazil: negotiating identity under stigma.
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Araújo MAL, Montagner MA, da Silva RM, Lopes FL, and de Freitas MM
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In Brazil, the inclusion of minorities and the most vulnerable members of the population in the health service is still a challenge for health planners. This study analyzed the perception of men who have sex with men (MSM) treated in health units with the onset of a sexually transmitted disease (STD). It consists of qualitative research involving 21 MSM, contacted in a non-governmental organization (NGO) and in the Testing and Counseling Center (TCC) in Fortaleza, Ceará, Brazil. The data were collected during two periods: from February until May 2007 and from September until October 2008 by using focused interview, and were organized by subject matter according to the content analysis technique of Bardin. The references to the theoretical concepts of the symbolic violence by Bourdieu and stigma by Goffman were the foundation for the analysis. The results indicate that MSM experienced situations of symbolic violence and avoided seeking primary health care service fearing prejudice and being stigmatized. Even when they sought these services, they tended to demonstrate masculine behavior in order to not be indentified as to their sexual orientation. We concluded that MSM feel excluded and discriminated against in the primary health care service. More studies related to this subject matter are needed to help implement health care policies facilitating the inclusion of and to welcome this group of the population into the general health care service. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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11. Psychopathological profile of 35% CO2 challenge test-induced panic attacks: a comparison with spontaneous panic attacks.
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Nardi AE, Valença AM, Lopes FL, Nascimento I, Veras AB, Freire RC, Mezzasalma MA, de-Melo-Neto VL, and Zin WA
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- 2006
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12. Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa
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Carlos Iracilda Z, Azevedo Isabel, Bolzani Vanderlan S, Silva Dulce HS, Regasini Luis O, Rocha Ana, Pirraco Ana, Lopes Flávia CM, and Soares Raquel
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Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt. Methods Human umbilical vein endothelial cells (HUVEC) were incubated with 8 μM Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor κB (NFκB), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean ± SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used. Results A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NFκB activity. Conclusion These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development.
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- 2009
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13. Body dysmorphic disorder treated with bupropion: cases report.
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Nardi AE, Lopes FL, and Valença AM
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- 2005
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14. Is there a hypersensitive visual alarm system in panic disorder?
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Roberto Teggi, Stefano Bondi, Massimiliano Grassi, Mario Bussi, Giampaolo Perna, Daniela Caldirola, Fabiana Leao Lopes, Caldirola, D, Teggi, R, Bondi, S, Lopes, Fl, Grassi, M, Bussi, Mario, Perna, G., Psychiatrie & Neuropsychologie, and RS: MHeNs School for Mental Health and Neuroscience
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Adult ,Male ,Balance ,medicine.medical_specialty ,genetic structures ,Vision ,Audiology ,Developmental psychology ,Young Adult ,medicine ,Humans ,Postural Balance ,Agoraphobia ,Biological Psychiatry ,Pain Measurement ,Balance (ability) ,Psychiatric Status Rating Scales ,Vestibular system ,Analysis of Variance ,Panic disorder ,Posturography ,Panic ,Middle Aged ,medicine.disease ,Psychiatry and Mental health ,Sensation Disorders ,Peripheral vision ,Visual Perception ,Panic Disorder ,Female ,Visual Fields ,medicine.symptom ,Psychology ,Photic Stimulation ,Anxiety disorder - Abstract
Agoraphobia in panic disorder (PD) has been related to abnormal balance system function. Vision influences balance and behavioural adaptations; peripheral vision influences orienting and fast defensive reactions whereas central vision analyzes details of objects. We have hypothesized that the abnormal balance function in PD could be mainly related to peripheral vision as part of a defensive alarm system in the brain. In 25 patients with PD and agoraphobia and 31 healthy controls we assessed, by posturography, balance system reactivity to video-films projected in peripheral and central visual fields (randomized sequence). Length, velocity and surface of body sway were calculated. Patients increased their body sway during peripheral stimulation, whereas controls did not; the two groups showed a similar increase of body sway during central stimulation. Anxiety levels during peripheral stimulation significantly influenced the postural response in the group of patients. These preliminary results suggest that the higher visual sensitivity to peripheral stimulation in patients with PD and agoraphobia may be linked to a more active "visual alarm system" involving visual, vestibular and limbic areas that might influence the development of agoraphobia in situations where environmental stimuli are uncertain.
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- 2011
15. 11C-Choline PET/CT for Restaging of Bladder Cancer
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Patrick M. Colletti, Stefano Fanti, Josè Chichero, Flávia Paiva Proença Lobo Lopes, Stefano Costa, Sotirios Chondrogiannis, Francesco Ceci, Lorenzo Bianchi, Domenico Rubello, Riccardo Schiavina, Paolo Castellucci, Tiziano Graziani, Graziani T, Ceci F, Lopes FL, Chichero J, Castellucci P, Schiavina R, Bianchi L, Chondrogiannis S, Colletti PM, Costa S, Rubello D, and Fanti S
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,Multimodal Imaging ,Choline ,medicine ,Humans ,11C-Choline PET/CT ,Radiology, Nuclear Medicine and imaging ,Carbon Radioisotopes ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Curative intent ,Bladder cancer ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,11c choline pet ct ,Radiation therapy ,Urinary Bladder Neoplasms ,Positron-Emission Tomography ,Female ,Radiology ,Radiopharmaceuticals ,Tomography, X-Ray Computed ,business - Abstract
AIM: The aim of this study was to evaluate of C-choline PET/CT in bladder cancer (BC) patients with suspected relapse after primary therapy. METHODS: Twenty-five BC patients with surgery (21 [84%]) or radiotherapy (4 [16%]) with curative intent had PET/CT for suspicion of relapse. Mean TNM was T2b N0 M0 (range, T1a N0 M0 to T4 N2 M0), whereas mean age was 71.3 years (range, 50-85 years). Nine (36%) of 25 were treated with adjuvant or salvage chemotherapy within 6 months before PET/CT. Positive findings were validated by histology or correlative imaging and/or clinical follow-up lasting at least 12 months. Age, TNM, histology, previous chemotherapy, and type of primary treatment were correlated with PET/CT positivity by univariate and multivariate binary logistic regression analysis. RESULTS: C-choline PET/CT was positive in 16 (64%) of 25. Six (37.5%) of 16 were positive in residual bladder/bladder bed, with 2 local false positive (FP) and 1 false negative (FN) on lymph nodes (LNs); 3 of 16 patients had PET-positive LNs with 1 FP; 1 of 16 patients showed distant metastases. Two (12.5%) of 16 had positive residual bladder/bladder bed and locoregional LNs; 1 (6.3%) of 16, residual bladder/bladder bed and bone; metastasis, 1 (6.3%) of 16 residual bladder/bladder bed and lung metastasis; 2 (12.5%) of 16, LN and distant metastasis. Five (56%) of 9 of PET negatives were FN in residual bladder/bladder bed. Eighteen (72%) of 25 were validated by histology, with 7 (18%) of 25 by correlative imaging and/or clinical follow-up. C-choline PET/CT was sensitive, specific, and accurate, with good positive and negative predictive values for local relapse of 66.7%, 84.6%, 76%, 80%, and 73.3% and 90%, 93.3%, 92%, 90%, and 93.3% for LNs and distant relapse, respectively. No FP or FN was detected for distant metastasis. None of the investigated factors were statistically significant. CONCLUSION: C-choline PET/CT is useful for restaging BC suspected of relapse, especially for the evaluation of LN or distant metastases.
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- 2015
16. Compliance, persistence, costs and quality of life in young patients treated with antipsychotic drugs: results from the COMETA study
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Paolo Cortesi, Claudio Mencacci, Miriam C. J. M. Sturkenboom, Elvezio Pirfo, Luciana Scalone, Lorenzo G. Mantovani, Patrizia Berto, Fabiana L Lopes, Maria Grazia Giustra, Ferrannini Luigi, Cortesi, Pa, Mencacci, C, Luigi, F, Pirfo, E, Berto, P, Sturkenboom, Mc, Lopes, Fl, Giustra, Mg, Mantovani, LORENZO GIOVANNI, Scalone, L., Medical Informatics, Cortesi, P, Ferrannini, L, Pirfo, L, Sturkenboom, M, Lopes, F, Giustra, M, Mantovani, L, and Scalone, L
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Quality of life ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Cost-Benefit Analysis ,Health Status ,medicine.medical_treatment ,Medication Adherence ,Persistence ,Indirect costs ,SDG 3 - Good Health and Well-being ,Cost of illne ,Internal medicine ,medicine ,Humans ,Longitudinal Studies ,Schizophreniform disorder ,Psychiatry ,Antipsychotic ,Cost–benefit analysis ,Medication compliance ,business.industry ,Health Care Costs ,medicine.disease ,Health Surveys ,Mental health ,Psychiatry and Mental health ,Schizophrenia ,Cost of illness ,Female ,Schizophrenic Psychology ,business ,Research Article ,Antipsychotic Agents ,Cohort study - Abstract
Background Little data is available on the real-world socio-economic burden and outcomes in schizophrenia. This study aimed to assess persistence, compliance, costs and Health-Related Quality-of-Life (HRQoL) in young patients undergoing antipsychotic treatment according to clinical practice. Methods A naturalistic, longitudinal, multicentre cohort study was conducted: we involved 637 patients aged 18–40 years, with schizophrenia or schizophreniform disorder diagnosed ≤10 years before, enrolled in 86 Italian Mental Health Centres and followed-up for 1 year. Comparisons were conducted between naïve (i.e., patients visiting the centre for the first time and starting a new treatment regimen) and non naïve patients. Results At enrolment, 84% of patients were taking atypical drugs, 3.7% typical, 10% a combination of the two classes, and 2% were untreated. During follow-up, 23% of patients switched at least once to a different class of treatment, a combination or no treatment. The mean Drug-Attitude-Inventory score was 43.4, with 94.3% of the patients considered compliant by the clinicians. On average, medical costs at baseline were 390.93€/patient-month, mostly for drug treatment (29.5%), psychotherapy (29.2%), and hospitalizations (27.1%). Patients and caregivers lost 3.5 days/patient-month of productivity. During follow-up, attitude toward treatment remained fairly similar, medical costs were generally stable, while productivity, clinical statusand HRQoL significantly improved. While no significantly different overall direct costs trends were found between naïve and non naïve patients, naïve patients showed generally a significant mean higher improvement of clinical outcomes, HRQoL and indirect costs, compared to the others. Conclusions Our results suggest how tailoring the treatment strategy according to the complex and specific patient needs make it possible to achieve benefits and to allocate more efficiently resources. This study can also provide information on the most relevant items to be considered when conducting cost-effectiveness studies comparing specific alternatives for the treatment of target patients.
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- 2013
17. Carbon dioxide-induced panic attacks and quantitative electroencephalogram in panic disorder patients
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Pedro Ribeiro, Fabiana L. Lopes, Roberto Piedade, Isabella Nascimento, Daniela Caldirola, Antonio Egidio Nardi, Giampaolo Perna, Alexandre Martins Valença, Rafael C. Freire, Walter A. Zin, Mariana Marques de Oliveira, Laura Bellodi, Lopes, Fl, Oliveira, Mm, Freire, Rc, Caldirola, D, Perna, G, Bellodi, Laura, Valenca, Am, Nascimento, I, Piedade, Ra, Ribeiro, P, Zin, Wa, and Nardi, Ae
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Adult ,Male ,medicine.medical_specialty ,Electroencephalography ,Audiology ,behavioral disciplines and activities ,Beta band ,Neuroimaging ,Double-Blind Method ,medicine ,Humans ,Beta wave ,Psychiatry ,Biological Psychiatry ,medicine.diagnostic_test ,Panic disorder ,Panic ,Brain ,Carbon Dioxide ,medicine.disease ,Frontal Lobe ,Psychiatry and Mental health ,Delta Rhythm ,Right posterior ,Anxiety ,Panic Disorder ,Female ,Occipital Lobe ,medicine.symptom ,Psychology ,Beta Rhythm - Abstract
The objective of the study was to investigate and compare the brain cortical activity, as indexed by quantitative electroencephalographic (qEEG) power, coherence and asymmetry measures, in panic disorder (PD) patients during an induced panic attack with a 35% CO(2) challenge test and also in a resting condition. Fifteen subjects with PD were randomly assigned to both 35% CO(2) mixture and atmospheric compressed air, in a double-blind study design, with EEG being recorded for a 20-min period. During induced panic attacks we found a reduced right-sided frontal orbital asymmetry in the beta band, a decreased occipital frontal intra-hemispheric coherence in the delta band at both right and left sides, a left-sided occipital delta inter-hemispheric asymmetry and an increased relative power in the beta wave at T4. Our data showed a disturbed frontal cortical processing, pointing to an imbalance of the frontal and occipital sites, common to both hemispheres, and an increased right posterior activity related to the high arousing panic attack condition. Those findings corroborate the Neuroanatomical hypothesis of PD.
- Published
- 2010
18. Sugar signaling modulates SHOOT MERISTEMLESS expression and meristem function in Arabidopsis .
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Lopes FL, Formosa-Jordan P, Malivert A, Margalha L, Confraria A, Feil R, Lunn JE, Jönsson H, Landrein B, and Baena-González E
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- Sucrose metabolism, Sugars metabolism, Light, Homeodomain Proteins, Arabidopsis metabolism, Arabidopsis genetics, Arabidopsis growth & development, Meristem metabolism, Meristem growth & development, Meristem genetics, Arabidopsis Proteins metabolism, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant, Signal Transduction, Protein Serine-Threonine Kinases metabolism, Protein Serine-Threonine Kinases genetics
- Abstract
In plants, development of all above-ground tissues relies on the shoot apical meristem (SAM) which balances cell proliferation and differentiation to allow life-long growth. To maximize fitness and survival, meristem activity is adjusted to the prevailing conditions through a poorly understood integration of developmental signals with environmental and nutritional information. Here, we show that sugar signals influence SAM function by altering the protein levels of SHOOT MERISTEMLESS (STM), a key regulator of meristem maintenance. STM is less abundant in inflorescence meristems with lower sugar content, resulting from plants being grown or treated under limiting light conditions. Additionally, sucrose but not light is sufficient to sustain STM accumulation in excised inflorescences. Plants overexpressing the α1-subunit of SUCROSE-NON-FERMENTING1-RELATED KINASE 1 (SnRK1) accumulate less STM protein under optimal light conditions, despite higher sugar accumulation in the meristem. Furthermore, SnRK1α1 interacts physically with STM and inhibits its activity in reporter assays, suggesting that SnRK1 represses STM protein function. Contrasting the absence of growth defects in SnRK1α1 overexpressors, silencing SnRK1α in the SAM leads to meristem dysfunction and severe developmental phenotypes. This is accompanied by reduced STM transcript levels, suggesting indirect effects on STM. Altogether, we demonstrate that sugars promote STM accumulation and that the SnRK1 sugar sensor plays a dual role in the SAM, limiting STM function under unfavorable conditions but being required for overall meristem organization and integrity under favorable conditions. This highlights the importance of sugars and SnRK1 signaling for the proper coordination of meristem activities., Competing Interests: Competing interests statement:The authors declare no competing interest.
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- 2024
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19. Brazilian mangrove sediments as a source of biosurfactant-producing yeast Pichia pseudolambica for bioremediation.
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Macedo Silva JR, Petra de Oliveira Barros V, Terceiro PS, Nunes de Oliveira Í, Francisco da Silva Moura O, Duarte de Freitas J, Crispim AC, Maciel Melo VM, Thompson FL, Maraschin M, and Landell MF
- Abstract
This work highlights the biosurfactant production potential of yeasts from mangroves in northeastern Brazil. The biosurfactants were evaluated by their emulsifying capacity (EI24), with 6 isolates showing values between 50% and 62%. Surfactant properties from crude extract were measured using drop collapse, oil displacement, Parafilm® M, surface tension and critical micellar concentration tests. The effects of temperature, salinity, pH, and the ability to emulsify different hydrocarbons were analyzed, showing a promising potential of the yeast species investigated to tolerance to high temperatures and acidic pH, in addition to emulsifying different sources of hydrocarbons with environmental impact. It is important to note that the Pichia pseudolambica isolates showed a remarkable ability to reduce the surface tension of water, from 70.82 mN/m to 36.47 mN/m. In addition, the critical micellar concentration (CMC) values ranged from 7 to 16 mg/mL, highlighting the promising surfactant activity of these isolates for future applications. It was identified that the biosurfactant adhered to the yeast cell wall, and FTIR and 1H NMR spectroscopy analysis was carried out on the yeast biomass and its post-sonication supernatant. The results indicate the presence of characteristic functional groups and peaks found in biosurfactants of a glycolipid nature. Taking together the results reveals the promising potential of biosurfactant biosynthesis of P. pseudolambica yeast, a trait not reported in the literature so far for this species. P. pseudolambica presents a relevant metabolic potential for alternative substrate use and resilience to adverse conditions that could enable it to produce biosurfactants for the biotechnological remediation of areas contaminated by oil derivatives. The metabolic properties herein investigated, together with their presence in Brazilian mangroves, make P. pseudolambica an emerging candidate for developing industrial processes and sustainable strategies for the recovery of ecosystems impacted by oil spills, being positioned as a sustainable alternative to conventional surfactants., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have influenced the work reported in this article., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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20. Enhancing the efficiency of magnetically driven carbon nitride-based nanocomposites with magnetic nanoflowers for the removal of methylene blue dye at neutral pH.
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Rodovalho FL, Rosa EV, da Silva AO, Moya SE, Campos AFC, and Sousa MH
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- Hydrogen-Ion Concentration, Adsorption, Water Purification methods, Coloring Agents chemistry, Graphite chemistry, Nitrogen Compounds, Methylene Blue chemistry, Nanocomposites chemistry, Water Pollutants, Chemical chemistry, Nitriles chemistry
- Abstract
The present study focuses on the elaboration of magnetic nanocomposites by the in situ incorporation of magnetite (Fe
3 O4 ) nanoparticles (NPs) with spherical and nanoflower-like morphologies in graphitic carbon nitride (g-C3 N4 ) sheets using two different synthetic routes. Nanomaterials are characterized by TEM, SEM, XRD, FTIR, BET, zetametry, vibrating sample magnetometry, and UV-vis absorption spectroscopy. The decoration of the carbon nitride matrix with the magnetic NPs enhanced optical and textural properties. The influence of the morphology of the magnetic NPs on the adsorptive and photocatalytic properties of the nanocomposites under different pH conditions (4.5, 6.9, and 10.6) was assessed from batch tests to remove methylene blue (MB) from aqueous solutions. In extreme pH conditions, the nanocomposites exhibited lower or equivalent MB removal capacity compared to the pure g-C3 N4 . However, at neutral medium, the nanocomposite with incorporated Fe3 O4 nanoflowers showed a significantly higher removal efficiency (80.7%) due to the combination of a high adsorption capacity and a good photocatalytic activity in this pH region. The proposed nanocomposite is a promising alternative to remove cationic dyes from water by magnetic assistance, since no pH adjustment of the polluted effluent is required, reducing costs and environmental impact in the dyeing industry., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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21. Spatially resolved distribution of pancreatic hormones proteoforms by MALDI-imaging mass spectrometry.
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Sisnande T, Brum FL, Matias DO, de Sá Ribeiro F, Moulin TB, Mohana-Borges R, de Magalhães MTQ, and Lima LMTR
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- Animals, Mice, Pancreatic Hormones metabolism, Islets of Langerhans metabolism, Mice, Inbred C57BL, Pancreas metabolism, Male, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Zinc analysis, Zinc metabolism
- Abstract
Zinc plays a crucial role both in the immune system and endocrine processes. Zinc restriction in the diet has been shown to lead to degeneration of the endocrine pancreas, resulting in hormonal imbalance within the β-cells. Proteostasismay vary depending on the stage of a pathophysiological process, which underscores the need for tools aimed at directly analyzing biological status. Among proteomics methods, MALDI-ToF-MS can serve as a rapid peptidomics tool for analyzing extracts or by histological imaging. Here we report the optimization of MALDI imaging mass spectrometry analysis of histological thin sections from mouse pancreas. This optimization enables the identification of the major islet peptide hormones as well as the major accumulated precursors and/or proteolytic products of peptide hormones. Cross-validation of the identified peptide hormones was performed by LC-ESI-MS from pancreatic islet extracts. Mice subjected to a zinc-restricted diet exhibited a relatively lower amount of peptide intermediates compared to the control group. These findings provide evidence for a complex modulation of proteostasis by micronutrients imbalance, a phenomenon directly accessed by MALDI-MSI., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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22. Leishmania infantum infection modulates messenger RNA, microRNA and long non-coding RNA expression in human neutrophils in vitro.
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Scaramele NF, Troiano JA, Felix JS, Costa SF, Almeida MC, Florencio de Athayde FR, Soares MF, Lopes MFDS, Furlan AO, Lima VMF, and Lopes FL
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- Humans, Male, Leishmaniasis, Visceral immunology, Leishmaniasis, Visceral parasitology, Leishmaniasis, Visceral genetics, Adult, Gene Expression Profiling, Neutrophils immunology, Neutrophils metabolism, Leishmania infantum genetics, Leishmania infantum immunology, RNA, Long Noncoding genetics, MicroRNAs genetics, RNA, Messenger genetics, RNA, Messenger metabolism
- Abstract
In the Americas, L. infantum (syn. chagasi) is the main cause of human visceral leishmaniasis. The role of neutrophils as part of the innate response to Leishmania spp. infection is dubious and varies according to the species causing the infection. Global expression of coding RNAs, microRNAs and long non-coding RNAs changes as part of the immune response against pathogens. Changes in mRNA and non-coding RNA expression resulting from infection by Leishmania spp. are widely studied in macrophages, but scarce in neutrophils, the first cell to encounter the trypanosomatid, especially following infection by L. infantum. Herein, we aimed to understand the expression patterns of coding and non-coding transcripts during acute in vitro infection of human neutrophils by L. infantum. We isolated neutrophils from whole blood of healthy male donors (n = 5) and split into groups: 1) infected with L. infantum (MOI = 5:1), and 2) uninfected controls. After 3 hours of exposure of infected group to promastigotes of L. infantum, followed by 17 hours of incubation, total RNA was extracted and total RNA-Seq and miRNA microarray were performed. A total of 212 genes were differentially expressed in neutrophils following RNA-Seq analysis (log2(FC)±0.58, FDR≤0.05). In vitro infection with L. infantum upregulated the expression of 197 and reduced the expression of 92 miRNAs in human neutrophils (FC±2, FDR≤0.01). Lastly, 5 downregulated genes were classified as lncRNA, and of the 10 upregulated genes, there was only 1 lncRNA. Further bioinformatic analysis indicated that changes in the transcriptome and microtranscriptome of neutrophils, following in vitro infection with L. infantum, may impair phagocytosis, apoptosis and decrease nitric oxide production. Our work sheds light on several mechanisms used by L. infantum to control neutrophil-mediated immune response and identifies several targets for future functional studies, aiming at the development of preventive or curative treatments for this prevalent zoonosis., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Scaramele et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2024
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23. Evaluation of chimeric recombinant antigens for the serodiagnosis of Trypanosoma cruzi in dogs: a promising tool for Chagas disease surveillance.
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Fontes ND, Habib FL, Leony LM, Freitas NEM, Silva ÂAO, Dantas-Torres F, da Silva Sales KG, da Câmara ACJ, de Araújo-Neto VT, Amorim LDAF, Celedon PAF, Zanchin NIT, and Santos FLN
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- Animals, Dogs, Recombinant Proteins immunology, Recombinant Proteins genetics, Chagas Disease diagnosis, Chagas Disease veterinary, Chagas Disease parasitology, Trypanosoma cruzi immunology, Trypanosoma cruzi genetics, Dog Diseases diagnosis, Dog Diseases parasitology, Antigens, Protozoan immunology, Antigens, Protozoan genetics, Serologic Tests methods, Serologic Tests veterinary, Enzyme-Linked Immunosorbent Assay methods, Enzyme-Linked Immunosorbent Assay veterinary, Sensitivity and Specificity, Antibodies, Protozoan blood
- Abstract
Background: Chagas disease (CD), a neglected parasitic disease caused by Trypanosoma cruzi, poses a significant health threat in Latin America and has emerged globally because of human migration. Trypanosoma cruzi infects humans and over 100 other mammalian species, including dogs, which are important sentinels for assessing the risk of human infection. Nonetheless, the serodiagnosis of T. cruzi in dogs is still impaired by the absence of commercial tests. In this study, we investigated the diagnostic accuracy of four chimeric recombinant T. cruzi IBMP antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) for detecting anti-T. cruzi antibodies in dogs, using latent class analysis (LCA)., Methods: We examined 663 canine serum samples, employing indirect ELISA with the chimeric antigens. LCA was utilized to establish a latent variable as a gold standard for T. cruzi infection, revealing distinct response patterns for each antigen., Results: The IBMP (Portuguese acronym for the Molecular Biology Institute of Paraná) antigens achieved area under the ROC curve (AUC) values ranging from 90.9% to 97.3%. The highest sensitivity was attributed to IBMP-8.2 (89.8%), while IBMP-8.1, IBMP-8.3, and IBMP-8.4 achieved 73.5%, 79.6%, and 85.7%, respectively. The highest specificity was observed for IBMP-8.4 (98.6%), followed by IBMP-8.2, IBMP-8.3, and IBMP-8.1 with specificities of 98.3%, 94.4%, and 92.7%, respectively. Predictive values varied according to prevalence, indicating higher effectiveness in endemic settings., Conclusions: Our findings underscore the remarkable diagnostic performance of IBMP-8.2 and IBMP-8.4 for the serodiagnosis of Trypanosoma cruzi in dogs, representing a promising tool for the diagnosis of CD in dogs. These chimeric recombinant antigens may not only enhance CD surveillance strategies but also hold broader implications for public health, contributing to the global fight against this neglected tropical disease., (© 2024. The Author(s).)
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- 2024
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24. Genome-wide association study of major anxiety disorders in 122,341 European-ancestry cases identifies 58 loci and highlights GABAergic signaling.
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Strom NI, Verhulst B, Bacanu SA, Cheesman R, Purves KL, Gedik H, Mitchell BL, Kwong AS, Faucon AB, Singh K, Medland S, Colodro-Conde L, Krebs K, Hoffmann P, Herms S, Gehlen J, Ripke S, Awasthi S, Palviainen T, Tasanko EM, Peterson RE, Adkins DE, Shabalin AA, Adams MJ, Iveson MH, Campbell A, Thomas LF, Winsvold BS, Drange OK, Børte S, Ter Kuile AR, Nguyen TH, Meier SM, Corfield EC, Hannigan L, Levey DF, Czamara D, Weber H, Choi KW, Pistis G, Couvy-Duchesne B, Van der Auwera S, Teumer A, Karlsson R, Garcia-Argibay M, Lee D, Wang R, Bjerkeset O, Stordal E, Bäckmann J, Salum GA, Zai CC, Kennedy JL, Zai G, Tiwari AK, Heilmann-Heimbach S, Schmidt B, Kaprio J, Kennedy MM, Boden J, Havdahl A, Middeldorp CM, Lopes FL, Akula N, McMahon FJ, Binder EB, Fehm L, Ströhle A, Castelao E, Tiemeier H, Stein DJ, Whiteman D, Olsen C, Fuller Z, Wang X, Wray NR, Byrne EM, Lewis G, Timpson NJ, Davis LK, Hickie IB, Gillespie NA, Milani L, Schumacher J, Woldbye DP, Forstner AJ, Nöthen MM, Hovatta I, Horwood J, Copeland WE, Maes HH, McIntosh AM, Andreassen OA, Zwart JA, Mors O, Børglum AD, Mortensen PB, Ask H, Reichborn-Kjennerud T, Najman JM, Stein MB, Gelernter J, Milaneschi Y, Penninx BW, Boomsma DI, Maron E, Erhardt-Lehmann A, Rück C, Kircher TT, Melzig CA, Alpers GW, Arolt V, Domschke K, Smoller JW, Preisig M, Martin NG, Lupton MK, Luik AI, Reif A, Grabe HJ, Larsson H, Magnusson PK, Oldehinkel AJ, Hartman CA, Breen G, Docherty AR, Coon H, Conrad R, Lehto K, Deckert J, Eley TC, Mattheisen M, and Hettema JM
- Abstract
The major anxiety disorders (ANX; including generalized anxiety disorder, panic disorder, and phobias) are highly prevalent, often onset early, persist throughout life, and cause substantial global disability. Although distinct in their clinical presentations, they likely represent differential expressions of a dysregulated threat-response system. Here we present a genome-wide association meta-analysis comprising 122,341 European ancestry ANX cases and 729,881 controls. We identified 58 independent genome-wide significant ANX risk variants and 66 genes with robust biological support. In an independent sample of 1,175,012 self-report ANX cases and 1,956,379 controls, 51 of the 58 associated variants were replicated. As predicted by twin studies, we found substantial genetic correlation between ANX and depression, neuroticism, and other internalizing phenotypes. Follow-up analyses demonstrated enrichment in all major brain regions and highlighted GABAergic signaling as one potential mechanism underlying ANX genetic risk. These results advance our understanding of the genetic architecture of ANX and prioritize genes for functional follow-up studies., Competing Interests: Per Hoffmann receives Salary from the Life & Brain GmbH, Bonn, Germany. James L. Kennedy is a member of the Scientific Advisory Board for Myriad Neuroscience Inc. Ian B. Hickie was an inaugural Commissioner on Australia’s National Mental Health Commission (2012-18). He is the Co-Director, Health and Policy at the Brain and Mind Centre (BMC) University of Sydney. The BMC operates an early-intervention youth services at Camperdown under contract to headspace. He is the Chief Scientific Advisor to, and a 5% equity shareholder in, InnoWell Pty Ltd. InnoWell was formed by the University of Sydney (45% equity) and PwC (Australia; 45% equity) to deliver the $30 M Australian Government-funded Project Synergy (2017-20; a three-year program for the transformation of mental health services) and to lead transformation of mental health services internationally through the use of innovative technologies. Andrew M. Mcintosh has received research support from Eli Lilly, Janssen, and The Sackler Trust. AMM has also received speaker fees from Illumina and Janssen. Murray B. Stein has in the past 3 years received consulting income from Acadia Pharmaceuticals, Aptinyx, atai Life Sciences, Boehringer Ingelheim, Bionomics, BioXcel Therapeutics, Clexio, Eisai, EmpowerPharm, Engrail Therapeutics, Janssen, Jazz Pharmaceuticals, and Roche/Genentech. Dr. Stein has stock options in Oxeia Biopharmaceuticals and EpiVario. He is paid for his editorial work on Depression and Anxiety (Editor-in-Chief), Biological Psychiatry (Deputy Editor), and UpToDate (Co-Editor-in-Chief for Psychiatry). He has also received research support from NIH, Department of Veterans Affairs, and the Department of Defense. He is on the scientific advisory board for the Brain and Behavior Research Foundation and the Anxiety and Depression Association of America. Joel Gelernter is named as an inventor on PCT patent application #15/878,640 entitled: “Genotype-guided dosing of opioid agonists,” filed January 24, 2018 and issued on January 26, 2021 as U.S. Patent No. 10,900,082; and is paid for editorial work for the journal “Complex Psychiatry.” Iiris Hovatta received speaker’s honoraria from Lundbeck. Ole A. Andreassen received speaker’s honorarium from Lundbeck and Sunovion, consultant for Cortechs.ai and Precision Health AS. Katharina Domschke has been a member of the Steering Committee Neurosciences, Janssen, Inc. until 2022 and is currently a member of the Board of the German National Society of Psychiatry (DGPPN) and the Neurotorium Editorial Board of the Lundbeck Foundation. Jordan W. Smoller is a member of the Scientific Advisory Board of Sensorium Therapeutics (with equity) and has received an honorarium for an internal seminar Tempus Labs. He is PI of a collaborative study of the genetics of depression and bipolar disorder sponsored by 23andMe for which 23andMe provides analysis time as in-kind support but no payments. Eduard Maron has received research support and has also received speaker fees from Lundbeck. Hans J. Grabe has received travel grants and speakers honoraria from Indorsia, Neuraxpharm, Servier and Janssen Cilag. Henrik Larsson has served as a speaker for Evolan Pharma, Medici and Shire/Takeda and has received research grants from Shire/Takeda; all outside the submitted work. Gerome Breen is an advisory board member for Compass Pathways. Jürgen Deckert is a member of the board of the German Society of Biological Psychiatry and is on the scientific advisory boards of non-profit organizations and foundations. Volker Arolt worked as an advisor for Sanofi-Adventis Germany. Zach Fuller and Xin Wang are employees of 23andMe and hold stock or stock options in 23andMe. All other authors have no competing interests to declare.
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- 2024
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25. Phylogenetic inferences based on distinct molecular markers reveals a novel Babesia (Babesia pantanalensis nov. sp.) and a Hepatozoon americanum-related genotype in crab-eating foxes (Cerdocyon thous).
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Calchi AC, Braga LQV, Bassini-Silva R, Castro-Santiago AC, Herrera HM, Soares JF, Barros-Battesti DM, Machado RZ, Rocha FL, and André MR
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- Animals, Brazil epidemiology, Eucoccidiida genetics, Eucoccidiida classification, Eucoccidiida isolation & purification, Cyclooxygenase 1 genetics, Polymerase Chain Reaction veterinary, HSP70 Heat-Shock Proteins genetics, Coinfection veterinary, Coinfection parasitology, Foxes parasitology, Canidae parasitology, Electron Transport Complex IV genetics, Phylogeny, Babesia genetics, Babesia classification, Babesia isolation & purification, RNA, Ribosomal, 18S genetics, Babesiosis parasitology, Babesiosis epidemiology, Coccidiosis veterinary, Coccidiosis parasitology, Coccidiosis epidemiology, Genotype, DNA, Protozoan chemistry, DNA, Protozoan isolation & purification
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Piroplasmids and Hepatozoon spp. Are apicomplexan protozoa that may cause disease in several canid species. The present study aimed to expand the knowledge on the diversity of piroplasmids and Hepatozoon in crab-eating foxes (Cerdocyon thous; n = 12) sampled in the Pantanal of Mato Grosso do Sul State, central-western Brazil. PCR assays based on the 18S rRNA were used as screening. Three (25%) and 11 (91.7%) were positive for piroplasmids and Hepatozoon spp., respectively. Co-infection was found in three C. thous. Phylogenetic analyses based on the near-complete 18S rRNA, cox-1 and hsp70 genes evidenced the occurrence of a novel of Babesia spp. (namely Babesia pantanalensis nov. sp.) closely related to Rangelia vitalii and Babesia sp. 'Coco'. This finding was supported by the genetic divergence analysis which showed (i) high divergence, ranging from 4.17 to 5.62% for 18 S rRNA, 6.16% for hps70 and 4.91-9.25% for cox-1 and (ii) the genotype network (which displayed sequences separated from the previously described Piroplasmida species by median vectors and several mutational events). Also, phylogenetic analysis based on the 18S rRNA gene of Hepatozoon spp. positioned the sequences obtained herein in a clade phylogenetically related to Hepatozoon sp. 'Curupira 2', Hepatozoon sp. detected in domestic and wild canids from Uruguay and Hepatozoon americanum. The present study described Babesia pantanalensis nov sp. and Hepatozoon closely related to H. americanum in crab-eating foxes from Brazil. Moreover, the coinfection by piroplasmids and Hepatozoon sp. for the first time in crab-eating foxes strongly suggesting that this wild canid species potentially acts as a bio-accumulate of hemoprotozoan in wild environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this article., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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26. RELATIONSHIP BETWEEN RESIDUAL DIURESIS AND SARCOPENIA IN PATIENTS ON HEMODIALYSIS.
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Rodrigues VGB, Silva WT, da Silva ACR, Campos PC, Santos LMM, Lacerda ACR, Alves FL, Maciel EHB, Prates MCSM, Costa HS, de Lima VP, Mendonça VA, and Figueiredo PHS
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Objective: To assess the association of residual diuresis with sarcopenia in patients with Chronic Kidney Disease (CKD) on hemodialysis., Methods: Through a cross-sectional study, patients on hemodialysis were subjected to a Dual Energy Radiologic Absorption (DEXA) exam to record muscle mass. Based on the volume of urine collected in 24 hours, patients were classified as anuric (diuresis ≤ 100 mL/day) or non-anuric (diuresis > 100 mL/day). Functional performance was evaluated by Short Physical Performance Battery (SPPB) and muscle strength by handgrip strength and 5-repetition sit-to-stand test. The association between the absence of residual urine and the presence of sarcopenia, low SPPB, and low muscle strength was analyzed using a binary logistic regression model., Results: Ninety-two patients, with a mean age of 54.4 years (95% CI 51.3 - 57.4) and with a mean diuresis volume of 476.3 mL/day (95% CI 320.4 - 632.2) were evaluated (48 anuric and 44 non-anuric). Anuric patients had a 2.77 (95% CI 1.14 - 6.73) times greater probability of sarcopenia and had a 3.55 (1.14 - 11.0) times greater probability of low SPPB, regardless of gender, age, and time on dialysis. Gender was the other associated variable for the presence of sarcopenia, with males having a 3.30 (95% CI 1.34 - 8.13) times higher risk. There were no associations with muscle strength., Conclusion: The absence of residual diuresis in patients on hemodialysis is associated with a higher risk of sarcopenia and low functional performance., (Copyright © 2024. Published by Elsevier Inc.)
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- 2024
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27. The genomic and phylogenetic analysis of Marseillevirus cajuinensis raises questions about the evolution of Marseilleviridae lineages and their taxonomical organization.
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de Azevedo BL, Queiroz VF, de Aquino ILM, Machado TB, de Assis FL, Reis E, Araújo Júnior JP, Ullmann LS, Colson P, Greub G, Aylward F, Rodrigues RAL, and Abrahão JS
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- Brazil, Evolution, Molecular, Genomics methods, Open Reading Frames, Phylogeny, RNA, Viral genetics, Genome, Viral, Viruses classification, Viruses genetics
- Abstract
Marseilleviruses (MsV) are a group of viruses that compose the Marseilleviridae family within the Nucleocytoviricota phylum. They have been found in different samples, mainly in freshwater. MsV are classically organized into five phylogenetic lineages (A/B/C/D/E), but the current taxonomy does not fully represent all the diversity of the MsV lineages. Here, we describe a novel strain isolated from a Brazilian saltwater sample named Marseillevirus cajuinensis. Based on genomics and phylogenetic analyses, M. cajuinensis exhibits a 380,653-bp genome that encodes 515 open reading frames. Additionally, M. cajuinensis encodes a transfer RNA, a feature that is rarely described for Marseilleviridae. Phylogeny suggests that M. cajuinensis forms a divergent branch within the MsV lineage A. Furthermore, our analysis suggests that the common ancestor for the five classical lineages of MsV diversified into three major groups. The organization of MsV into three main groups is reinforced by a comprehensive analysis of clusters of orthologous groups, sequence identities, and evolutionary distances considering several MsV isolates. Taken together, our results highlight the importance of discovering new viruses to expand the knowledge about known viruses that belong to the same lineages or families. This work proposes a new perspective on the Marseilleviridae lineages organization that could be helpful to a future update in the taxonomy of the Marseilleviridae family., Importance: Marseilleviridae is a family of viruses whose members were mostly isolated from freshwater samples. In this work, we describe the first Marseillevirus isolated from saltwater samples, which we called Marseillevirus cajuinensis . Most of M. cajuinensis genomic features are comparable to other Marseilleviridae members, such as its high number of unknown proteins. On the other hand, M. cajuinensis encodes a transfer RNA, which is a gene category involved in protein translation that is rarely described in this viral family. Additionally, our phylogenetic analyses suggested the existence of, at least, three major Marseilleviridae groups. These observations provide a new perspective on Marseilleviridae lineages organization, which will be valuable in future updates to the taxonomy of the family since the current official classification does not capture all the Marseilleviridae known diversity., Competing Interests: The authors declare no conflict of interest.
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- 2024
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28. Anti-RBD IgG antibodies from endemic coronaviruses do not protect against the acquisition of SARS-CoV-2 infection among exposed uninfected individuals.
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Adami FL, de Castro MV, Almeida BDS, Daher IP, Yamamoto MM, Souza Santos K, Zatz M, Naslavsky MS, Rosa DS, Cunha-Neto E, de Oliveira VL, Kalil J, and Boscardin SB
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- Humans, Male, Female, Adult, Middle Aged, Coronavirus immunology, Endemic Diseases, Cross Reactions immunology, COVID-19 immunology, COVID-19 prevention & control, SARS-CoV-2 immunology, Immunoglobulin G immunology, Immunoglobulin G blood, Antibodies, Viral immunology, Antibodies, Viral blood, Spike Glycoprotein, Coronavirus immunology
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Background: The Coronaviridae family comprises seven viruses known to infect humans, classified into alphacoronaviruses (HCoV-229E and HCoV-NL63) and betacoronaviruses (HCoV-OC43 and HCoV-HKU1), which are considered endemic. Additionally, it includes SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East respiratory syndrome), and the novel coronavirus SARS-CoV-2, responsible for COVID-19. SARS-CoV-2 induces severe respiratory complications, particularly in the elderly, immunocompromised individuals and those with underlying diseases. An essential question since the onset of the COVID-19 pandemic has been to determine whether prior exposure to seasonal coronaviruses influences immunity or protection against SARS-CoV-2., Methods: In this study, we investigated a cohort of 47 couples (N=94), where one partner tested positive for SARS-CoV-2 infection via real-time PCR while the other remained negative. Plasma samples, collected at least 30 days post-PCR reaction, were assessed using indirect ELISA and competition assays to measure specific antibodies against the receptor-binding domain (RBD) portion of the Spike (S) protein from SARS-CoV-2, HCoV-229E, HCoV-NL63, HCoV-OC43, and HCoV-HKU1., Results: IgG antibody levels against the four endemic coronavirus RBD proteins were similar between the PCR-positive and PCR-negative individuals, suggesting that IgG against endemic coronavirus RBD regions was not associated with protection from infection. Moreover, we found no significant IgG antibody cross-reactivity between endemic coronaviruses and SARS-CoV-2 RBDs., Conclusions: Taken together, results suggest that anti-RBD antibodies induced by a previous infection with endemic HCoVs do not protect against acquisition of COVID-19 among exposed uninfected individuals., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Adami, de Castro, Almeida, Daher, Yamamoto, Souza Santos, Zatz, Naslavsky, Rosa, Cunha-Neto, de Oliveira, Kalil and Boscardin.)
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- 2024
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29. Biosurfactants production by marine yeasts isolated from zoanthids and characterization of an emulsifier produced by Yarrowia lipolytica LMS 24B.
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Petra de Oliveira Barros V, Macedo Silva JR, Maciel Melo VM, Terceiro PS, Nunes de Oliveira I, Duarte de Freitas J, Francisco da Silva Moura O, Xavier de Araújo-Júnior J, Erlanny da Silva Rodrigues E, Maraschin M, Thompson FL, and Landell MF
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- Surface-Active Agents chemistry, Kerosene, Hydrocarbons metabolism, Carbon metabolism, Biodegradation, Environmental, Yarrowia metabolism, Petroleum analysis
- Abstract
The present study investigates the potential for biosurfactant production of 19 marine yeast species obtained from zoanthids. Using the emulsification index test to screen the samples produced by the marine yeasts, we verified that five isolates exhibited an emulsification index ≥50%. Additional tests were performed on such isolates, including oil displacement, drop collapse, Parafilm M assay, and surface tension measurement. The tolerance of produced biosurfactants for environmental conditions was also analyzed, especially considering the media's temperature, pH, and salinity. Moreover, the surfactant's ability to emulsify different hydrocarbon sources and to metabolize kerosene as the sole carbon source was evaluated in vitro. Our results demonstrate that yeast biosurfactants can emulsify hydrocarbon sources under different physicochemical conditions and metabolize kerosene as a carbon source. Considering the Yarrowia lipolytica LMS 24B as the yeast model for biosurfactant production from the cell's wall biomass, emulsification indexes of 61.2% were obtained, even at a high temperature of 120 °C. Furthermore, the Fourier-transform middle infrared spectroscopy (FTIR) analysis of the biosurfactant's chemical composition revealed the presence of distinct functional groups assigned to a glycoprotein complex. Considering the status of developing new bioproducts and bioprocesses nowadays, our findings bring a new perspective to biosurfactant production by marine yeasts, especially Y. lipolytica LMS 24B. In particular, the presented results validate the relevance of marine environments as valuable sources of genetic resources, i.e., yeast strains capable of metabolizing and emulsifying petroleum derivatives., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have influenced the work reported in this article., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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30. Performance Assessment of Treponemal and Nontreponemal Tests for the Diagnosis of Acquired Syphilis.
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Silva ÂAO, Lima AA, Vasconcelos LCM, de Almeida RA, de Freitas NEM, Habib FL, Oliva TA, da Silva MFCR, de Siqueira IC, and Santos FLN
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- Humans, Male, Antibodies, Bacterial blood, Reagent Kits, Diagnostic standards, Female, Fluorescent Treponemal Antibody-Absorption Test, Adult, Syphilis diagnosis, Syphilis blood, Syphilis Serodiagnosis methods, Syphilis Serodiagnosis standards, Sensitivity and Specificity, Enzyme-Linked Immunosorbent Assay methods, Treponema pallidum immunology, Treponema pallidum isolation & purification
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There are a variety of nontreponemal test (NTT) and treponemal test (TT) kits for the serologic diagnosis of syphilis. Because of the complexity of the infection (multiple clinical stages) and the different antigens used in these kits, a systematic evaluation of the accuracy of the currently available commercial tests is warranted. Our objective was to evaluate the performance of commercially available tests for the diagnosis of syphilis infection. In this study, we analyzed one NTT (Venereal Disease Research Laboratory [VDRL] test, Wiener Laboratories, Rosario, Argentina) and two TTs (fluorescent treponemal antibody absorption [FTA-ABS] test, Euroimmun, Lübeck, Germany, and syphilis recombinant ELISA v. 4.0 test [ELISA], Wiener Laboratories, Rosario, Argentina) using a panel of 187 samples, including serum samples from 31 individuals with primary syphilis, 77 with secondary syphilis, and 79 with latent syphilis. An additional 192 samples from uninfected individuals and 323 serum samples from individuals with other diseases were included. The sensitivities of the VDRL, ELISA, and FTA-ABS tests were 97.9%, 100%, and 96.3%, respectively. The VDRL and ELISA tests showed a specificity of 100%, and the FTA-ABS test showed a specificity of 99.5%. Accuracy was 98.9% for the VDRL test, 100% for the ELISA, and 97.9% for the FTA-ABS test. For primary, secondary, and latent syphilis, the ELISA achieved a diagnostic performance of 100%, whereas the sensitivity for the VDRL and FTA-ABS tests ranged from 96.8% to 98.7% and 93.7% to 98.7%, respectively. No difference was observed when the tests were used as traditional or reverse algorithms. In general, all three tests are able to discriminate positive and negative samples for syphilis, regardless of the diagnostic algorithm.
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- 2024
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31. Comparing the activity of propolis-loaded nanoparticles or hydroethanolic extract on cytokine production by peripheral blood mononuclear cells.
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Sartori AA, de Oliveira Cardoso E, Santiago KB, Conte FL, Tasca KI, Justino IA, Marincek A, Marcato PD, Bastos JK, and Sforcin JM
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- Leukocytes, Mononuclear, Gene Expression, Cytokines, Propolis pharmacology, Nanoparticles
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- 2024
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32. Impact of historical soil management on the interaction of plant-growth-promoting bacteria with maize (Zea mays L.).
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Guidinelle RB, Burak DL, Rangel OJP, Peçanha AL, Passos RR, Rocha LOD, Olivares FL, and Mendonça ES
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Edaphic factors can modulate the effects of microbial inoculants on crop yield promotion. Given the potential complexity of microbial inoculant responses to diverse soil management practices, we hypothesize that sustainable management of soil and water irrigation may improve soil quality and enhance the effects of plant growth-promoting bacteria (PGPB). Consequently, the primary objective was to assess the effectiveness of microbial inoculants formulated with Herbaspirillum seropedicae (Hs) and Azospirillum brasilense (Ab) on maize growth in soils impacted by different historical conservation management systems. We evaluated two soil management systems, two irrigation conditions, and four treatments: T0 - without bioinoculant and 100% doses of NPK fertilization; T1 - Hs + humic substances and 40% of NPK fertilization; T2 - Ab and 40% of NPK fertilization; T3 - co-inoculation (Hs + Ab) and 40% of NPK fertilization. Using a reduced fertilization dose (40% NPK) associated with microbial inoculants proved efficient in increasing maize shoot dry mass : on average, there was a 16% reduction compared to the treatment with 100% fertilization. In co-inoculation (Hs + Ab), the microbial inoculants showed a mutualistic effect on plant response, higher than isolate ones, especially increasing the nitrogen content in no-tillage systems irrigated by swine wastewater. Under lower nutrient availability and higher biological soil quality, the microbial bioinputs positively influenced root development, instantaneous water use efficiency, stomatal conductance, and nitrogen contents., Competing Interests: The authors declare that there is no competing interest in this research., (© 2024 The Authors. Published by Elsevier Ltd.)
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- 2024
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33. Activity of extracts and terpenoids from Tontelea micrantha (Mart. ex Schult.) A.C.Sm. (Celastraceae) against pathogenic bacteria.
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Lopes RP, Ferreira FL, Faria de Sousa G, Cruz Nizer WSD, Magalhães CLB, Ferreira JMS, Tótola AH, Duarte LP, and de Magalhães JC
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The pharmacological properties of plant extracts and phytochemicals, such as flavonoids and terpenoids, remain of great interest. In this work, the effect of extracts, friedelan-3,21-dione, and 3β- O -D-glucosyl-sitosterol isolated from Tontelea micrantha roots was evaluated against Staphylococcus aureus , Bacillus subtilis , Klebsiella pneumoniae , Klebsiella oxytoca and Escherichia coli . The antibacterial activity was evaluated by the minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively), and the synergistic effect was assessed by the Checkerboard assay. Furthermore, the cytotoxicity of the plant-derived compounds against Vero cells was measured by the 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide (MTT) method. The biological effects of the isolated compounds were predicted using the PASS online software. The chloroform and hexane extracts of T. micrantha roots showed promising antibacterial effect, with MIC in the range of 4.8-78.0 µg/mL. Further analyses showed that these compounds do not affect the integrity of the membrane. The combination with streptomycin strongly reduced the MIC of this antibiotic and extracts. The extracts were highly toxic to Vero cells, and no cytotoxicity was detected for the two terpenoids isolated from them (i.e. friedelan-3,21-dione and 3β- O -D-glucosyl-sitosterol; CC
50 > 1000 μg/mL). Therefore, extracts obtained from T. micrantha roots significantly inhibited bacterial growth and are considered promising agents against pathogenic bacteria. The cytotoxicity results were very relevant and can be tested in bioassays.- Published
- 2024
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34. Brazilian Guideline for Exercise Test in the Adult Population - 2024.
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Carvalho T, Freitas OGA, Chalela WA, Hossri CAC, Milani M, Buglia S, Precoma DB, Falcão AMGM, Mastrocola LE, Castro I, Albuquerque PF, Coutinho RQ, Brito FS, Alves JC, Serra SM, Santos MAD, Colombo CSSS, Stein R, Herdy AH, Silveira ADD, Castro CLB, Silva MMFD, Meneghello RS, Ritt LEF, Malafaia FL, Marinucci LFB, Pena JLB, Almeida AEM, Vieira MLC, and Stier Júnior AL
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- Humans, Brazil, Adult, Female, Male, Cardiovascular Diseases, Exercise Test standards, Exercise Test methods
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- 2024
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35. Reliability and validity of the Patient Generated Index (PGI) in patients with chronic kidney disease (CKD) on dialysis, a new approach to quality of life.
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Campos PC, Silva ACRD, Fleury Cardoso R, Rodrigues VGB, Alves FL, Maciel EHB, Prates MCSM, Costa HS, Figueiredo PHS, and Lima VP
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- Adult, Humans, Quality of Life psychology, Reproducibility of Results, Emotions, Surveys and Questionnaires, Renal Dialysis, Renal Insufficiency, Chronic therapy
- Abstract
Purpose: To evaluate the test-retest reliability and validity of the Patient Generated Index (PGI) in individuals with Chronic Kidney Disease (CDK) undergoing hemodialysis., Methods: Through a non-experimental study with repeated measures, PGI was applied twice to assess internal consistency and test-retest reliability. Correlations with the Kidney Disease Quality of Life Short Form (KDQOL-SF), the Human Activity Profile (HAP) questionnaire, the Social Participation Scale, and the Glittre ADL Test were used., Results: 91 individuals with CKD were evaluated. There was high reliability for the PGI (ICC= 0.97) PGI correlated with KQDOL - SF in Functional Capacity r = 0.38 ( p < 0.001), Emotional Well-Being r = 0.31 ( p = 0.003), Social Aspect r = 0.22 ( p = 0.036), Emotional Function r = 0.22 ( p = 0.038) and Effect of Kidney Disease r = 0.21 ( p = 0.042), and Physical scores r = 0.24 ( p = 0.021)), Mental r = 0.21 ( p = 0.05) and General r = 0.22 ( p = 0.037) summarized. There was a significant correlation between PGI and HAP r = 0.40 ( p < 0.001) and the Social Participation Scale r = -0.36 ( p < 0.001). There was no correlation between the PGI and Glittre ADL scores r = 0.12 ( p = 0.247)., Conclusion: In adults receiving hemodialysis, the PGI proved to be an accurate and reliable instrument for the assessment of the quality of life from the perspective of the patient.IMPLICATIONS FOR REHABILITATIONAlthough hemodialysis treatment is associated with increased survival and symptom control, there is a significant change in the patient's lifestyle.In order to provide a more focused view of the individual, the Patient Generated Index (PGI) was created to evaluate the quality of life.PGI is reliable and correlates with KQDOL - SF and the Social Participation Scale in this population.
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- 2024
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36. Biofortification with selenium as an alternative to increase the total phenolic compounds in brassicas: a systematic review and meta-analysis.
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Kamchen CM, de Oliveira FL, de Souza TR, Vieira BS, Telles B, and Morzelle MC
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- Humans, Antioxidants, Biofortification methods, Phenols analysis, Brassica chemistry, Selenium analysis
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The ability of brassicas to accumulate selenium is crucial for their positive effects on health. Selenium improves the immune system and the antioxidant defenses. Selenium biofortification of brassicas has therefore been explored to increase dietary selenium intake in humans. However, the effects of selenium biofortification on bioactive compounds, mainly phenolic compounds, are not clear. So, this systematic review and meta-analysis aimed to answer the question 'What are effects of the biofortification of brassicas with selenium on total phenolic compounds?' Ten studies, which assessed the effect of selenium biofortification on total phenolic compounds, were selected for qualitative synthesis and four studies were included in the meta-analysis after a thorough literature review of the PubMed, Science Direct, and Web of Knowledge databases. The quality of the evidence ranged from high to moderate. The meta-analysis results indicated that the total phenolic compound content was significantly higher (P = 0.002) in the supplemented group but the results showed considerable heterogeneity (P < 0.00001, I
2 = 97%) between studies. This systematic review and meta-analysis summarizes the effect of Se biofortification on the increase in the content of total phenolic compounds and it suggests that several factors can affect this relationship. © 2023 Society of Chemical Industry., (© 2023 Society of Chemical Industry.)- Published
- 2024
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37. Metabolic disease and the liver: A review.
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Vargas M, Cardoso Toniasso SC, Riedel PG, Baldin CP, Dos Reis FL, Pereira RM, Brum MCB, Joveleviths D, and Alvares-da-Silva MR
- Abstract
Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common liver disease worldwide, with an estimated prevalence of 31% in Latin America. The presence of metabolic comorbidities coexisting with liver disease varies substantially among populations. It is acknowledged that obesity is boosting the type 2 diabetes mellitus "epidemic," and both conditions are significant contributors to the increasing number of patients with MASLD. Non-alcoholic steatohepatitis represents a condition of chronic liver inflammation and is considered the most severe form of MASLD. MASLD diagnosis is based on the presence of steatosis, noninvasive scores and altered liver tests. Noninvasive scores of liver fibrosis, such as serum biomarkers, which should be used in primary care to rule out advanced fibrosis, are simple, inexpensive, and widely available. Currently, guidelines from international hepatology societies recommend using noninvasive strategies to simplify case finding and management of high-risk patients with MASLD in clinical practice. Unfortunately, there is no definite pharmacological treatment for the condition. Creating public health policies to treat patients with risk factors for MASLD prevention is essential., Competing Interests: Conflict-of-interest statement: All the authors declare that they have no conflicts of interest., (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
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- 2024
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38. Age- and sex-specific normative values for muscle mass parameters in 18,625 Brazilian adults.
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Coelho-Júnior HJ, Marques FL, Sousa CV, Marzetti E, and Aguiar SDS
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- Male, Adult, Humans, Female, Middle Aged, Muscle, Skeletal pathology, Muscle, Skeletal physiology, Brazil, Exercise, Sarcopenia
- Abstract
Background: The present study aimed to provide age- and sex-specific normative values for muscle mass parameters in Brazilian adults., Methods: Data pertaining to Brazilian adults (18+ years) who attended a nutritional clinical between January 2018 and July 2022 were analyzed. Muscle mass parameters were assessed using a bioimpedance digital scale (InBody 230, GBC BioMed NZ). Assessments were conducted under standard conditions, with participants refraining from physical exercise for 96 h and from eating or drinking (including water) for 8 h before evaluations., Results: A total of 18,625 Brazilian adults were analyzed. Normative values for absolute and relative (height, m
2 ) muscle mass and appendicular muscle mass (ASM) were calculated. In addition, specific age-related changes in muscle mass parameters were observed. In women, muscle mass peaked between the ages of 40-49 before gradually declining at an average rate of 5.7% per decade from the sixth decade of life onwards. ASM reached its peak earlier, during the third decade of life, and started to decline later, from 50 to 59 years. In contrast, absolute and ASM peaked at 40-49 years and declined from the sixth decade of life in men. Both sexes displayed a slightly greater decline in ASM than in muscle mass (13 vs. 12%)., Conclusions: The present study provides normative values for absolute and relative muscle mass and ASM in Brazilian adults. Furthermore, important specific age-related changes in muscle mass parameters were observed. These data have public health implications and might serve as a reference tool to guide health professionals., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author (s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Coelho-Júnior, Marques, Sousa, Marzetti and Aguiar.)- Published
- 2024
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39. Prostate histological investigation in rats exposed to bisphenol a and phytochemicals during the perinatal period and subjected to hormonal stimulus in adulthood.
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Cavalleri Sousa T, de Oliveira CB, Silva Ricardo ML, Musa de Aquino A, Scarano WR, Cruz Veras AS, Almeida Tavares ME, Teixeira GR, Castillho ACD, Pacagnelli FL, Zalotti Brandt J, and de Oliveira Mendes L
- Subjects
- Male, Pregnancy, Female, Rats, Animals, Genistein pharmacology, Benzhydryl Compounds toxicity, Phytochemicals pharmacology, Collagen pharmacology, Prostate pathology, Estrogens, Phenols
- Abstract
Bisphenol A (BPA) is an environmentally dispersed chemical associated with tumor development. Phytochemicals such as indole-3-carbinol (I3C) and genistein (GEN) have chemoprotective effects on tumor cells. Thus, this study aimed to evaluate the prostatic morphological aspects of rats exposed to BPA, GEN, and I3C during the perinatal period and submitted to hormonal stimulus in adulthood. Blood was collected to obtain hormone concentrations. Slides stained with hematoxylin & eosin, and picrosirius were subjected to fractal, stereological, morphometric, and collagen quantification analysis. I3C decreased the plasma dihydrotestosterone levels, and both phytochemicals increased the plasma estrogen levels. Unlike phytochemicals, BPA did not alter any of the parameters evaluated. GEN reduced the epithelial height, while I3C increased the fractal dimension and stromal collagen. Although BPA did not alter the prostate morphology, the phytochemicals provided beneficial effects for the prostate histological organization in adult animals subjected to hormonal stimulus.
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- 2024
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40. Effects of long-acting injectable progesterone supplementation at early dioestrus on pregnancy maintenance in beef and dairy recipient cattle.
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Neto AL, Ruas FL, Santo BSE, Mattos ACD, Silva JB, Júnior MFD, Pereira DFC, Lacerda IP, Carvalho JO, and Pugliesi G
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- Pregnancy, Cattle, Animals, Female, Pregnancy Rate, Pregnancy Maintenance, Dietary Supplements, Estrus Synchronization methods, Progesterone pharmacology, Insemination, Artificial veterinary
- Abstract
We tested in the present study the hypothesis that supplementation with long-acting P4 (iP4) at different times of the initial dioestrus improves pregnancy rates in dairy and beef recipients submitted to fixed-time embryo transfer (FTET). Recipients from commercial farms had their oestrous cycle synchronized with an E2/P4-based protocol in three experiments (Exp. 1 to 3). In Exp. 1, dairy heifers (n = 76) and cows (n = 104) were randomly assigned to two experimental groups: the control group (n = 89) and the iP4D4 group (n = 91). For Exps. 2 and 3, suckled beef recipients were used. In Exp. 2, recipients were assigned to two experimental groups: Control group (n = 147) and iP4D7 group (n = 144); whereas in Exp. 3, recipients were randomly assigned to three experimental groups: Control group (n = 85), iP4-D4 group (n = 86) and iP4D7 group (n = 81). Recipients in the iP4D4 and iP4-D7 groups received an i.m. administration of 150 mg iP4, on D4 or D7 (D0 was the day of expected oestrus). On D7, all recipients were evaluated by transrectal ultrasonography and those that had a CL received a fresh or vitrified in vitro-produced embryo. In Exp. 2 and 3, the CL area was also determined by ultrasonography at the time of FTET. The pregnancy diagnosis was performed at 30 days in Exp. 1, 57 days in Exp. 2, and between 40 and 72 days of pregnancy in Exp. 3. In Exp. 1, the pregnancy rate did not differ (p > .1) between the Control group (38.2% [34/89]) and iP4D4 group (49.5% [45/91]); yet, a parity effect indicated a greater (p < .05) pregnancy rate in heifers (57.9% [44/76]) than cows (30.8% [32/104]). In Exp. 2, the pregnancy rate was greater (p < .05) in the iP4D7 group (45.0% [65/144]) than in the Control group (34.0% [50/147]). Also, a greater (p = .08) pregnancy rate was observed for recipients with a small CL (≤2.75 cm
2 ) that were treated with iP4 on the day of FTET than the control recipients (46.4% [32/69] vs. 32.6% [28/86]). In Exp. 3, no significant effects (p > .1) of the treatment group or CL size were detected on pregnancy rates at days 30 and 60. In conclusion, the beneficial effects of iP4 supplementation at early dioestrus on pregnancy maintenance may vary according to the experimental conditions, but its use at the time of FTET can be used as an alternative to enhance the fertility of beef recipients in challenging conditions in commercial herds., (© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.)- Published
- 2024
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41. Preoperative respiratory muscle training reduces the risk of pulmonary complications and the length of hospital stay after cardiac surgery: a systematic review.
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Cursino de Moura JF, Oliveira CB, Coelho Figueira Freire AP, Elkins MR, and Pacagnelli FL
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- Adult, Humans, Length of Stay, Breathing Exercises methods, Respiratory Muscles physiology, Postoperative Complications prevention & control, Cardiac Surgical Procedures adverse effects, Pneumonia prevention & control
- Abstract
Questions: What is the effect of preoperative respiratory muscle training (RMT) on the incidence of postoperative pulmonary complications (PPCs) after open cardiac surgery? What is the effect of RMT on the duration of mechanical ventilation, postoperative length of stay and respiratory muscle strength?, Design: Systematic review of randomised trials with meta-analysis., Participants: Adults undergoing elective open cardiac surgery., Intervention: The experimental groups received preoperative RMT and the comparison groups received no intervention., Outcome Measures: The primary outcomes were PPCs, length of hospital stay, respiratory muscle strength, oxygenation and duration of mechanical ventilation. The methodological quality of studies was assessed using the PEDro scale and the overall certainty of the evidence was assessed using the GRADE approach., Results: Eight trials involving 696 participants were included. Compared with the control group, the respiratory training group had fewer PPCs (RR 0.51, 95% CI 0.38 to 0.70), less pneumonia (RR 0.44, 95% CI 0.25 to 0.78), shorter hospital stay (MD -1.7 days, 95% CI -2.4 to -1.1) and higher maximal inspiratory pressure values at the end of the training protocol (MD 12 cmH
2 O, 95% CI 8 to 16). The mechanical ventilation time was similar in both groups. The quality of evidence was high for pneumonia, length of hospital stay and maximal inspiratory pressure., Conclusion: Preoperative RMT reduced the risk of PPCs and pneumonia after cardiac surgery. The training also improved the maximal inspiratory pressure and reduced hospital stay. The effects on PPCs were large enough to warrant use of RMT in this population., Registration: CRD42021227779., (Copyright © 2023 Australian Physiotherapy Association. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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42. Croton cajucara : Patents and Nanotechnological Advances.
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Lima LR, da Silva Júnior FL, Arcanjo DDR, and Medeiros Maciel MA
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- Humans, Nanotechnology, COVID-19 Drug Treatment, Plant Extracts chemistry, Plant Extracts therapeutic use, Plant Extracts pharmacology, Animals, SARS-CoV-2 drug effects, Phytotherapy, Patents as Topic, Croton chemistry, Diterpenes, Clerodane chemistry, Diterpenes, Clerodane therapeutic use, Diterpenes, Clerodane isolation & purification
- Abstract
Croton cajucara Benth showed several pharmacological properties such as: antiinflammatory, antinociceptive hypoglycemic, lipid-lowering, antiulcer, antiestrogenic, antitumor, antigenotoxic, antimutagenic, and cardiovascular. The 19- nor -clerodane diterpene transdehydrocrotonin ( t -DCTN or DCTN) is the major bioactive constituent extracted from the bark of this Croton . Patents for Croton cajucara Benth in the period 2015 to 2022 comprises 14 published documents. Among them 4 patents are colloidal systems (SM/SNEDDS) loading t -DCTN for pharmacological applications. Patent registrations highlighted the huge promising biotechnological potential of Croton cajucara Benth especially in the phytotherapy field, and the correlation with its bioactive constituents of which t -DCTN showed the foremost results, so this herbal could become an alternative in the treatment of COVID-19. However, investigation of more recently published patents for clerodane diterpenes with similar chemical structure of t -DCTN, who previously showed antiviral property must be carried out and should be searched on several patent data bases., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Published
- 2024
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43. Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil.
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Soares Júnior JC, Itoyama BF, Beretta BMS, Hossotani CMS, Silva MSC, Silva GSD, Nakamura AA, Lopes FL, and Meireles MV
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- Animals, Chickens parasitology, Brazil, Poultry genetics, Nigeria, DNA, Protozoan genetics, Eimeria genetics, Coccidiosis diagnosis, Coccidiosis epidemiology, Coccidiosis veterinary, Poultry Diseases epidemiology, Poultry Diseases parasitology
- Abstract
The objective of this study was to identify Eimeria spp. in alternative poultry production systems (APPS) in the State of São Paulo, Brazil. Fecal samples (168) and DNA extracted from fecal samples obtained in APPS located in different Municipalities in the State of São Paulo (93) were examined by microscopy or genera-specific PCR (ITS-1 locus). Samples positive for Eimeria spp. were examined using Eimeria lata, Eimeria nagambie, and Eimeria zaria species-specific PCR protocols (ITS-2 locus) and another E. lata-specific PCR (candidate IMP1 genomic locus) followed by molecular cloning (E. lata and E. zaria ITS-2 amplicons) and genetic sequencing. All positive DNA samples were also submitted to genera-specific nested PCR (18S rRNA gene) followed by next-generation sequencing to identify Eimeria spp. Eimeria nagambie, E. zaria, and Eimeria sp. were identified by ITS2-targeted species-specific PCRs and genetic sequencing. Next-generation sequencing identified, in order of prevalence: E. nagambie; Eimeria acervulina; Eimeria mivati; Eimeria praecox; Eimeria brunetti; Eimeria mitis; Eimeria sp.; Eimeria maxima; E. zaria, and Eimeria necatrix/tenella. Our results confirmed, for the first time in Brazil, the identification of E. nagambie, E. zaria, and Eimeria spp. ITS-2 and 18S rRNA gene sequences not yet described in Brazil.
- Published
- 2023
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44. Genome-wide significant risk loci for mood disorders in the Old Order Amish founder population.
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Humphries EM, Ahn K, Kember RL, Lopes FL, Mocci E, Peralta JM, Blangero J, Glahn DC, Goes FS, Zandi PP, Kochunov P, Van Hout C, Shuldiner AR, Pollin TI, Mitchell BD, Bucan M, Hong LE, McMahon FJ, and Ament SA
- Subjects
- Humans, Male, Female, Middle Aged, Adult, Case-Control Studies, Transcription Factors genetics, Risk Factors, Genetic Loci, Repressor Proteins genetics, Aged, Homeodomain Proteins genetics, Founder Effect, Nuclear Proteins genetics, Genome-Wide Association Study methods, Genetic Predisposition to Disease genetics, Mood Disorders genetics, Polymorphism, Single Nucleotide genetics, Amish genetics
- Abstract
Genome-wide association studies (GWAS) of mood disorders in large case-control cohorts have identified numerous risk loci, yet pathophysiological mechanisms remain elusive, primarily due to the very small effects of common variants. We sought to discover risk variants with larger effects by conducting a genome-wide association study of mood disorders in a founder population, the Old Order Amish (OOA, n = 1,672). Our analysis revealed four genome-wide significant risk loci, all of which were associated with >2-fold relative risk. Quantitative behavioral and neurocognitive assessments (n = 314) revealed effects of risk variants on sub-clinical depressive symptoms and information processing speed. Network analysis suggested that OOA-specific risk loci harbor novel risk-associated genes that interact with known neuropsychiatry-associated genes via gene interaction networks. Annotation of the variants at these risk loci revealed population-enriched, non-synonymous variants in two genes encoding neurodevelopmental transcription factors, CUX1 and CNOT1. Our findings provide insight into the genetic architecture of mood disorders and a substrate for mechanistic and clinical studies., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
- Published
- 2023
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45. Freshwater decapod crustaceans from the state of Minas Gerais, Brazil: species composition and distribution.
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Jacobucci GB, Bueno AAP, Almeida AC, Alves DFR, Barros-Alves SP, Magalhes C, Souza-Carvalho EA, Mossolin EC, Carvalho FL, and Mantelatto FL
- Subjects
- Humans, Animals, Brazil, Fresh Water, Decapoda, Palaemonidae, Anomura
- Abstract
Brazil contains about 5% (~120 spp.) of the total known species of freshwater decapods. Information on the occurrence of freshwater decapod species in the state of Minas Gerais is dispersed and much of this remains unpublished. We hereby propose to present the first inventory of freshwater decapods from the state, based on data compiled from publications and scientific collections and obtained from fieldwork in the state. Eleven species of freshwater decapods were recorded in Minas Gerais, including three species of crabs (Trichodactylidae), seven of shrimps (Palaemonidae) and one species of freshwater squat lobster (Aeglidae), classified into three infraorders, three families, and four genera. The genus Macrobrachium C.S. Bate, 1868 comprised most of the decapod records. The reduced number of species in relation to coastal states is related, at least in part, to the absence of amphidromic species. Environmental degradation and the construction of dams in several watersheds may be possible causes of this absence. The records obtained from surveys carried out in national parks situated in Minas Gerais and its surroundings indicate the importance of these areas for the conservation of decapod fauna in the state.
- Published
- 2023
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46. Co-expression analysis suggests lncRNA-mRNA interactions enhance antiviral immune response during acute Chikungunya fever in whole blood of pediatric patients.
- Author
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Felix JS, Almeida MC, Lopes MFDS, de Athayde FRF, Troiano JA, Scaramele NF, Furlan AO, and Lopes FL
- Subjects
- Male, Adolescent, Humans, Child, RNA, Messenger genetics, RNA, Messenger metabolism, Transcriptome, Immunity, Innate genetics, Gene Expression Profiling, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Chikungunya Fever genetics
- Abstract
Chikungunya virus is an arbovirus that causes the neglected tropical disease chikungunya fever, common in tropical areas worldwide. There is evidence that arboviruses alter host transcriptome and modulate immune response; this modulation may involve transcriptional and post-transcriptional control mechanisms mediated by long non-coding RNAs (lncRNAs). Herein, we employed bioinformatic analysis to evaluate co-expression of lncRNAs and their putative target mRNAs in whole blood during natural Chikungunya infection in adolescent boys. Sequencing data from GSE99992 was uploaded to the Galaxy web server, where data was aligned with HISAT2, gene counts were estimated with HTSeq-count, and differential expression was run with DESeq2. After gene classification with Biomart, Pearson's correlation was applied to identify potential interactions between lncRNAs and mRNAs, which were later classified into cis and trans according to genomic location (FEELnc) and binding potential (LncTar), respectively. We identified 1,975 mRNAs and 793 lncRNAs that were differentially expressed between the acute and convalescent stages of infection in the blood. Of the co-expressed lncRNAs and mRNAs, 357 potentially interact in trans and 9 in cis; their target mRNAs enriched pathways related to immune response and viral infections. Out of 52 enriched KEGG pathways, the RIG-I like receptor signaling is enriched by the highest number of target mRNAs. This pathway starts with the recognition of viral pathogens, leading to innate immune response mediated by the production of IFN-I and inflammatory cytokines. Our findings indicate that alterations in lncRNA expression in adolescent boys, induced by acute Chikungunya infection, potentially modulate mRNAs that contribute to antiviral immune responses., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Felix et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
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47. Comparative study using propensity score matching analysis in patients undergoing surgery for colorectal cancer with or without multimodal prehabilitation.
- Author
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Estrada DML, de Queiroz FL, Guerra LI, França-Neto PR, Lacerda-Filho A, de Miranda Silvestre SC, and Coelho JM
- Subjects
- Humans, Propensity Score, Patient Readmission, Postoperative Complications etiology, Postoperative Complications prevention & control, Preoperative Exercise, Colorectal Neoplasms surgery
- Abstract
Introduction: The complication rate after major abdominal surgery is from 35 to 50%. The multimodal prehabilitation covers all the pre-operative problems to guarantee a faster recovery and reduce the rate of morbidity and mortality after a colorectal procedure., Methods: Observational study, in patients with CRC who underwent surgical treatment between November 2020 and September 2022. The data of the patients were placed in 2 groups: prehabilitation group (PPH) and no prehabilitation group (NPPH). Demographic data, type of cancer, operative data, and postoperative data were collected. Characteristics between the groups were compared after a propensity score matching (PSM) analysis for the detection of differences., Results: After the PSM analysis, 46 patients were in PPH, and 63 patients were in NPPH. There was no significant difference in postoperative complications (p = 0.192). The median of comprehensive complication index (CCI) was 0 (p = 0.552). Patients in the NPPH had more hospital readmissions (p = 0.273) and more emergency room visits (p = 0.092). Multivariate log binomial regression adjusted for complications showed that pre-habilitation reduces the risk of a pos-operative complication (OR: 0.659, 95%CI, 0.434-1.00, p = 0.019)., Conclusions: The postoperative complication rate and LOS were similar between patients who receive operative multimodal prehabilitation for CRC surgery and those who did not. Prehabilitation was associated with reduced risk of postoperative complication after multivariate log binomial regression adjusted for complications. Patients who underwent prehabilitation had a slightly lower tendency for postoperative ER visits and hospital readmissions., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
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48. Polyp detection in the cecum and ascending colon by dye based chromoendoscopy - Is its routine use justified?
- Author
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Paiva RA, Queiroz FL, França Neto PR, Costa BXMD, Cardoso LAB, Estrada DML, Mota FFD, and Lacerda Filho A
- Subjects
- Humans, Colon, Ascending pathology, Indigo Carmine, Prospective Studies, Colonoscopy, Cecum pathology, Colonic Polyps diagnosis, Colonic Polyps pathology, Adenoma diagnosis, Adenoma pathology
- Abstract
Introduction: colonoscopy is the best method for detecting polyps, with a reduction in colorectal cancer mortality of 29% and reaching 47% for distal tumors. However, it fails to demonstrate a significant reduction in proximal colon cancer mortality, and is the most common segment with interval neoplasm. The present study aimed to evaluate the impact on detection of polyps of a second sequential evaluation of cecum and ascending colon, with or without the use of indigo carmine chromoendoscopy., Methods: prospective, non-randomized clinical trial. Patients were divided into two groups. The first (G1) underwent a routine colonoscopy, followed by a second endoscopy assessment of ascending colon and cecum. The second group (G2) underwent a routine colonoscopy, followed by a second assessment of the ascending colon and cecum with indigo carmine chromoendoscopy., Results: In total, 203 patients were analyzed, 101 in the G1 and 102 in the G2. Newer polyps were identified in both groups after the second assessment with a significantly higher number of polyps detected in the patients in the G2 (p=0.0001). The number of patients who had at least one polyp in the two endoscopic assessments was significantly higher in the G2 (53 or 52% vs 27 or 26.7%, p=0.0002). In the second endoscopic assessment, the number of polyps found was also significantly higher in the G2 (50 or 76.9%) compared to the G1 (15 or 23.1%), p<0.0001., Conclusions: the second assessment with dye-based chromoendoscopy increases the detection of polyps in the ascending colon and cecum.
- Published
- 2023
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49. Fractal analysis is a useful tool for evaluating prostate tissue remodeling caused by ethanol consumption and androgen therapy.
- Author
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Lima BJP, de Oliveira GRL, Sousa TC, de Aquino AM, Hinokuma KD, Ricardo MLS, Scarano WR, Castilho ACS, Pacagnelli FL, Martinez FE, and Mendes LO
- Abstract
Alcohol has been widely consumed for centuries and is linked to the aggravation of diseases. Several studies have shown that excessive consumption of ethanol results in morphophysiological changes in the male reproductive system. One of the effects of ethanol is the decrease in testosterone concentration and hormonal therapies are an alternative to minimize the changes resulting from chronic alcoholism. Qualitative studies were commonly carried out to evaluate the male histopathological alterations resulting from ethanol consumption, being necessary quantitative and non-subjective techniques. This study analyzes the importance of fractal analysis as a useful tool to identify and quantify tissue remodeling in rats submitted to ethanol consumption and hormone therapy with testosterone. Prostate of animals submitted to chronic ethanol consumption showed tissue disorganization, which was confirmed by an increasing of fractal dimension. Regarding the prostatic stroma, collagen fractal dimension and quantification revealed lower values in animals that were only submitted to androgen therapy. Thus, we can conclude that the fractal analysis was a useful tool to quantify tissue changes caused by ethanol consumption and androgen therapy., Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
- Published
- 2023
- Full Text
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50. Glucocorticoid Receptor Gene ( NR3C1 ) Polymorphisms and Metabolic Syndrome: Insights from the Mennonite Population.
- Author
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Kolb KL, Mira ALS, Auer ED, Bucco ID, de Lima E Silva CE, Dos Santos PI, Hoch VB, Oliveira LC, Hauser AB, Hundt JE, Shuldiner AR, Lopes FL, Boysen TJ, Franke A, Pinto LFR, Soares-Lima SC, Kretzschmar GC, and Boldt ABW
- Subjects
- Humans, DNA Methylation genetics, Genotype, Glucocorticoids, Ethnicity, Metabolic Syndrome genetics, Receptors, Glucocorticoid genetics, Receptors, Glucocorticoid metabolism
- Abstract
The regulation of the hypothalamic-pituitary-adrenal (HPA) axis is associated with polymorphisms and the methylation degree of the glucocorticoid receptor gene ( NR3C1 ) and is potentially involved in the development of metabolic syndrome (MetS). In order to evaluate the association between MetS with the polymorphisms, methylation, and gene expression of the NR3C1 in the genetically isolated Brazilian Mennonite population, we genotyped 20 NR3C1 polymorphisms in 74 affected (MetS) and 138 unaffected individuals without affected first-degree relatives (Co), using exome sequencing, as well as five variants from non-exonic regions, in 70 MetS and 166 Co, using mass spectrometry. The methylation levels of 11 1F CpG sites were quantified using pyrosequencing (66 MetS and 141 Co), and the NR3C1 expression was evaluated via RT-qPCR (14 MetS and 25 Co). Age, physical activity, and family environment during childhood were associated with MetS. Susceptibility to MetS, independent of these factors, was associated with homozygosity for rs10482605*C (OR = 4.74, p corr = 0.024) and the haplotype containing TTCGTTGATT ( rs3806855*T_ rs3806854*T_rs10482605*C_rs10482614*G_rs6188*T_rs258813*T_rs33944801*G_rs34176759*A_rs17209258*T_rs6196*T , OR = 4.74, p corr = 0.048), as well as for the CCT haplotype ( rs41423247*C_ rs6877893*C_rs258763*T ), OR = 6.02, p corr = 0.030), but not to the differences in methylation or gene expression. Thus, NR3C1 polymorphisms seem to modulate the susceptibility to MetS in Mennonites, independently of lifestyle and early childhood events, and their role seems to be unrelated to DNA methylation and gene expression.
- Published
- 2023
- Full Text
- View/download PDF
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