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5. Study of the activity of Punica granatum-mediated silver nanoparticles against Candida albicans and Candida glabrata, alone or in combination with azoles or polyenes.

Author

Souza, José António Santos, Alves, Marta M, Barbosa, Debora Barros, Lopes, Maria Manuel, Pinto, Eugénia, Figueiral, Maria Helena, Delbem, Alberto Carlos B, and Mira, Nuno Pereira

Abstract

The continuous emergence of Candida strains resistant to currently used antifungals demands the development of new alternatives that could reduce the burden of candidiasis. In this work silver nanoparticles synthesized using a green route are efficiently used, alone or in combination with fluconazole, amphotericin B or nystatine, to inhibit growth of C. albicans and C. glabrata oral clinical strains, including in strains showing resistance to fluconazole. A potent inhibitory effect over biofilm formation prompted by the two Candida species was also observed, including in mature biofilm cells. These results foster the use of phytotherapeutics as effective treatments in oral candidiasis. [ABSTRACT FROM AUTHOR]

Published
2020
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6. Comparative genomic and transcriptomic analyses unveil novel features of azole resistance and adaptation to the human host in Candida glabrata

Author

Salazar, Sara Barbosa, primary, Wang, Can, additional, Münsterkötter, Martin, additional, Okamoto, Michiyo, additional, Takahashi-Nakaguchi, Azusa, additional, Chibana, Hiroji, additional, Lopes, Maria Manuel, additional, Güldener, Ulrich, additional, Butler, Geraldine, additional, and Mira, Nuno Pereira, additional

Published
2017
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7. A comunicação de más notícias na perspetiva dos profissionais de saúde

Author

Lopes, Maria Manuel Mateus Marques Claro, Sá, Luís Octávio de, and Coelho, Silvia Patricia Fernandes

Subjects
Revelação, Dizer a verdade, Questões de fim-de-vida, Breaking bad news, End-of-life issues, Ciências Médicas::Ciências da Saúde [Domínio/Área Científica], Communication in health care setting, Disclosure, Truth telling, Comunicação de más notícias, Comunicação em saúde
Abstract

Submitted by Margarida Vilhena Carvalho (mvc@ucp.pt) on 2022-01-20T16:52:31Z No. of bitstreams: 1 201498839.pdf: 3909186 bytes, checksum: f1ed29ce23984468c361e7c0a5582993 (MD5) Made available in DSpace on 2022-01-20T16:52:32Z (GMT). No. of bitstreams: 1 201498839.pdf: 3909186 bytes, checksum: f1ed29ce23984468c361e7c0a5582993 (MD5) Previous issue date: 2014-05-29

Published
2013

13. Antioxidant and Antimycotic Activities of Two Native Lavandula Species from Portugal.

Author

Baptista, Rafael, Madureira, Ana Margarida, Jorge, Rita, Adão, Rita, Duarte, Aida, Duarte, Noélia, Lopes, Maria Manuel, and Teixeira, Generosa

Subjects
THERAPEUTIC use of antioxidants, FLAVONOIDS, ALTERNATIVE medicine, ANALYSIS of variance, ANTIFUNGAL agents, BIOLOGICAL assay, DNA, ESSENTIAL oils, MEDICINAL plants, LIPID peroxidation (Biology), PHENOLS, STATISTICS, PLANT extracts, DATA analysis, DATA analysis software, FREE radical scavengers, DESCRIPTIVE statistics, THERAPEUTICS
Abstract

The antioxidant and antimycotic activities of the essential oils and extracts of two native Portuguese Lavandula species, L. stoechas subsp. luisieri and L. pedunculata, were evaluated by in vitro assays. The total phenolics and flavonoids content were also determined. The antioxidant potential was assessed through DPPH radical scavenging, inhibition of lipid peroxidation (ILP), and DNA protection assays. All samples displayed a high DPPH scavenging activity, some of them showing concentration dependence. The majority of the samples were also able to inhibit lipid peroxidation. A strong correlation was observed between the results of DPPH and ILP assays and the flavonoids content of the samples. In the DNA protection assay, all the extracts were able to preserve DNA integrity. The antimycotic activity was performed against twelve fungi belonging to Basidiomycota and Ascomycota Divisions. L. stoechas subsp. luisieri exhibited the broadest activity spectra. L. pedunculata extracts were active against five fungi. Cryptococcus neoformans was the most sensitive, being inhibited by all the extracts. Our results led to the conclusion that L. stoechas subsp. luisieri and L. pedunculata can be useful as new sources of natural antioxidants and antimycotic agents, providing a possible valorization of the existing biodiversity and resources of Portuguese flora. [ABSTRACT FROM AUTHOR]

Published
2015
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14. Hipoglucemia sin cetosis. Caso clínico

Author

Ferraz, Claudia, primary, Limões Role Reis, Marta Esmeralda, additional, Lopes, Maria Manuel, additional, Moral Westerman Cardoso, Maria Luís, additional, and Barbosa, Célia Rosa, additional

Published
2005
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18. Epidemiological survey of the pattern of colonization and infection caused by species of the Candida genus in Hospitals of the Lisbon area: emphasis on azole resistance and emergence of non-conventional species

Author

Mendonça, Catarina Ribeiro, Mira, Nuno Pereira, and Lopes, Maria Manuel Pereira

Subjects
Ciências da Saúde, Mestrado Integrado - 2019, Candidose, Candidemia, Colonização por Candida, Candida albicans, Espécies de Candida não-albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Resistência aos antifúngicos, Candida africana
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2019 Made available in DSpace on 2020-05-04T22:59:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-13 Instituto Superior Técnico da Universidade de Lisboa; Farmácia Ibéria

Published
2019

19. Classification of dermatophytes by a multilocus phylogenetic approach based on tef-1α, beta tubulin and its genes

Author

Tomé, Carolina Pedro Gomes, Sacheli, Rosalie, and Lopes, Maria Manuel Pereira

Subjects
Trichophyton mentagrophytes, Ciências da Saúde, Mestrado Integrado - 2019, Tinea capitis, Trichophyton interdigitale, Trichophyton violaceum, Trichophyton soudanense
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2019 Made available in DSpace on 2020-05-04T22:59:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-30 Centre Hospitalier Universitaire de Liège; Farmácia Oriental de Lisboa; Hospital Cuf Descobertas

Published
2019

20. Efeito inibitório de compostos naturais em culturas planctónicas e biofilmes de Candida

Author

Balau, Bruna Filipa Mendes, Silva, João Inácio, and Lopes, Maria Manuel

Subjects
natural products, Candida
Abstract

"The incidence and prevalence of invasive fungal infections has been increasing, especially in immunocompromised and hospitalized patients with serious underlying diseases. Several species of Candida are the main etiological agents of these types of infection, particularly Candida albicans. Pathogenicity of Candida species is attributed to several virulence factors including the capability to form biofilms, which show higher levels of antifungal resistance. The use of natural products, such as extracts of plant’s essential oils, have been explored as alternative antifungal therapeutic strategies. However, these naturally occurring compounds have not yet found any established role in the formulation of conventional pharmaceutical antifungal products. In addition, most published works assessed the antifungal activity of these compounds in planktonic yeast cells, existing a knowledge gap on the potential action these compounds may have against yeast biofilms.This work aimed to assess the antifungal activity of extracts of essential oils, and the eventual synergy these compounds may have with conventional antifungal drugs, against isolates of Candida growing as planktonic cultures and as biofilms.(...)" N/A

Published
2018

21. Estudo epidemiológico do isolamento de espécies do género Candida em amostras recolhidas em Hospitais da zona de Lisboa

Author

Velez, Noémi Magro, Mira, Nuno Pereira, and Lopes, Maria Manuel

Subjects
Mestrado Integrado - 2017, Ciências da Saúde, Candidíase, C. albicans, Resistência a antifúngicos, Candidemia, Candida não-albicans
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2017 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2018-12-21T15:14:13Z No. of bitstreams: 1 MICF_Noemi_Magro_Velez.pdf: 2086310 bytes, checksum: e842f246f17b919e846a7908197cabbb (MD5) Made available in DSpace on 2018-12-21T15:49:32Z (GMT). No. of bitstreams: 1 MICF_Noemi_Magro_Velez.pdf: 2086310 bytes, checksum: e842f246f17b919e846a7908197cabbb (MD5) Previous issue date: 2017-12-28

Published
2017

22. Dermatophytes identification and susceptibility testing

Author

Henriques, Cátia Sofia Ministro, Lopes, Maria Manuel, and Hayette, Marie-Pierre

Subjects
Ciências da Saúde, Mestrado Integrado - 2016
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2016 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2018-12-10T16:27:06Z No. of bitstreams: 1 MICF_Catia_Henriques.pdf: 1711895 bytes, checksum: 115be3fadc4ae6a77dda78be4d29da7d (MD5) Made available in DSpace on 2018-12-10T17:36:44Z (GMT). No. of bitstreams: 1 MICF_Catia_Henriques.pdf: 1711895 bytes, checksum: 115be3fadc4ae6a77dda78be4d29da7d (MD5) Previous issue date: 2016-11-25

Published
2016

23. Infecções fúngicas superficiais prevalentes nos atletas

Author

Costa, Liliana Sofia Matos da and Lopes, Maria Manuel

Subjects
Ciências da Saúde, Mestrado Integrado - 2014
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014 Made available in DSpace on 2017-03-06T18:36:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2015

Published
2015

24. Aspetos epidemiológicos da doença de Chagas no Mundo

Author

Costa, Joana Rita da Silva and Lopes, Maria Manuel

Subjects
Ciências da Saúde, Trypanosoma cruzi, Transmissão, Triatomíneos, Epidemiologia, Doença de Chagas, Mestrado Integrado - 2015
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Made available in DSpace on 2017-03-07T09:54:17Z (GMT). No. of bitstreams: 3 MICF_Joana_Costa.pdf: 598130 bytes, checksum: d1283ff18936145dc1f528dea1dd0305 (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Joana_Costa.pdf: 598130 bytes, checksum: d1283ff18936145dc1f528dea1dd0305 (MD5) Previous issue date: 2015

Published
2015

25. Relatório de Estágio: Laboratório de Patologia Clínica do IPO de Lisboa [Relatório de estágio]

Author

Correia, Estela de Jesus Guerreiro Batista Pinheiro, Vieira, Cidália, Meireles, Glória, Lourenço, Cesaltina, Coimbra, Filomena, Ornelas, Carmo, and Lopes, Maria Manuel

Subjects
Bioquímica, Virologia, Teses de mestrado - 2015, Ciências da Saúde, SIDA, Ciências Médicas, Imunologia, HIV, Infeções Fúngicas, Controlo de qualidade
Abstract

Relatório de estágio de mestrado, Análises Clínicas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2015-12-10T13:21:10Z No. of bitstreams: 2 Monografia (2).pdf: 7480425 bytes, checksum: 285597b117e68f8ef16cba9b6e8b0e67 (MD5) Relatório final.pdf: 7722738 bytes, checksum: 8e59b3dbe73063e3269ca67b0e00c039 (MD5) Made available in DSpace on 2015-12-10T13:21:32Z (GMT). No. of bitstreams: 2 Monografia (2).pdf: 7480425 bytes, checksum: 285597b117e68f8ef16cba9b6e8b0e67 (MD5) Relatório final.pdf: 7722738 bytes, checksum: 8e59b3dbe73063e3269ca67b0e00c039 (MD5) Previous issue date: 2015

Published
2015

26. Impacto ambiental do uso de fungicidas na agricultura

Author

Grilo, Regina Sofia Carminhos and Lopes, Maria Manuel

Subjects
Ambiente, Ciências da Saúde, Fungicidas, Agricultura, Resistências, Fungos, Ecossistemas, Mestrado Integrado - 2015
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Made available in DSpace on 2017-03-07T10:09:12Z (GMT). No. of bitstreams: 3 MICF_Regina_Grilo.pdf: 848287 bytes, checksum: 7f6c7795c6927a5cf643def57f1e1da7 (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Regina_Grilo.pdf: 848287 bytes, checksum: 7f6c7795c6927a5cf643def57f1e1da7 (MD5) Previous issue date: 2015

Published
2015

27. Cryptosporidium e Criptosporidiose

Author

Teixeira, Diana Lercas and Lopes, Maria Manuel

Subjects
Ciências da Saúde, Cryptosporidium, Paramomicina nitazoxanida, Imunocomprometidos, Criptosporidiose, Mestrado Integrado - 2015
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Made available in DSpace on 2017-03-07T11:00:51Z (GMT). No. of bitstreams: 3 MICF_Diana_Teixeira.pdf: 961906 bytes, checksum: f3fb326b008d02749d3fc2ad6d9bd9ff (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Diana_Teixeira.pdf: 961906 bytes, checksum: f3fb326b008d02749d3fc2ad6d9bd9ff (MD5) Previous issue date: 2015

Published
2015

28. O impacto das micotoxinas na segurança alimentar

Author

Mariano, Maria Duarte Carrasco Godinho and Lopes, Maria Manuel

Subjects
Micotoxinas, Ciências da Saúde, Micotoxicose, Aflatoxinas, Saúde pública, Patulina, Fungos, Mestrado Integrado - 2015
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Made available in DSpace on 2017-03-07T10:19:53Z (GMT). No. of bitstreams: 3 MICF_Maria_Mariano.pdf: 846611 bytes, checksum: 13b49075076175c65b36813652a7869b (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Maria_Mariano.pdf: 846611 bytes, checksum: 13b49075076175c65b36813652a7869b (MD5) Previous issue date: 2015

Published
2015

29. Papel do farmacêutico na prevenção e controlo da infeção nosocomial

Author

Mira, Lara Sofia Margarido Loureiro and Lopes, Maria Manuel

Subjects
Ciências da Saúde, Farmacêutico, Infeção, Controlo, Mestrado Integrado - 2014, Prevenção, Risco
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014 Made available in DSpace on 2017-03-06T19:01:23Z (GMT). No. of bitstreams: 3 MICF_Lara_Mira.pdf: 1281586 bytes, checksum: e5c218d46ee8f9b58b3d19a05858e4e9 (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Lara_Mira.pdf: 1281586 bytes, checksum: e5c218d46ee8f9b58b3d19a05858e4e9 (MD5) Previous issue date: 2015

Published
2015

30. Contaminação Ambiental Fúngica em Unidades de Cuidados de Saúde

Author

Silva, Filipa Tatiana Peixoto Serra Saudade e and Lopes, Maria Manuel

Subjects
Qualidade do ar interior, Ciências da Saúde, Fungos, Infeção fúngica invasiva, Infeção associada a cuidados de saúde, Mestrado Integrado - 2014
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2016-12-29T18:00:55Z No. of bitstreams: 1 MICF_Filipa_Saudade_Silva.pdf: 529505 bytes, checksum: 7326ba9a18928ab89ae555ca2c09ad2b (MD5) Made available in DSpace on 2017-01-04T17:25:06Z (GMT). No. of bitstreams: 1 MICF_Filipa_Saudade_Silva.pdf: 529505 bytes, checksum: 7326ba9a18928ab89ae555ca2c09ad2b (MD5) Previous issue date: 2014

Published
2014

31. Recursos Alternativos para o Tratamento de Fungos

Author

Sousa, Diana Lopes de and Lopes, Maria Manuel

Subjects
Micoses sistémicas e superficiais, Ciências da Saúde, Recursos alternativos, Mestrado Integrado - 2014
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2016-12-30T13:06:57Z No. of bitstreams: 1 MICF_Diana_Sousa.pdf: 1233792 bytes, checksum: 151310cdb67d4557883ad85120037f99 (MD5) Made available in DSpace on 2017-01-04T17:31:04Z (GMT). No. of bitstreams: 1 MICF_Diana_Sousa.pdf: 1233792 bytes, checksum: 151310cdb67d4557883ad85120037f99 (MD5) Previous issue date: 2014

Published
2014

32. Biofilmes fúngicos : impacto em saúde pública

Author

Gonçalves, Rita Monteiro Macedo Ribeiro and Lopes, Maria Manuel

Subjects
Ciências da Saúde, Resistência aos fármacos, Candida albicans, Candida não-albicans, Antifúngicos, Biofilmes, Mestrado Integrado - 2015
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015 Made available in DSpace on 2017-03-06T18:50:26Z (GMT). No. of bitstreams: 3 MICF_Rita_Goncalves.pdf: 958574 bytes, checksum: 69b6cd9f08c3c03879b167530dba88ec (MD5) license.txt: 1195 bytes, checksum: 25733b0b84e8845b1d09ead22e7c5a2c (MD5) MICF_Rita_Goncalves.pdf: 958574 bytes, checksum: 69b6cd9f08c3c03879b167530dba88ec (MD5) Previous issue date: 2015

Published
2014

33. Resistência aos antifúngicos e medidas de prevenção

Author

Cairrão, Tiago Alexandre Marques and Lopes, Maria Manuel Pereira

Subjects
Terapêutica, Micoses, Ciências Médicas::Ciências da Saúde [Domínio/Área Científica], Resistência, Epidemiologia, Prevenção, Mestrado Integrado - 2013
Abstract

Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2013 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2021-02-03T16:35:33Z No. of bitstreams: 2 MICF_Tiago_Cairrao.pdf: 828306 bytes, checksum: d06679f72dabc1715b96a6f003011c64 (MD5) MICF_Tiago_Cairrao_PPT.pdf: 2079037 bytes, checksum: 2e8f9bc92a76436d27f6a7cc91f8501a (MD5) Made available in DSpace on 2021-02-03T20:12:57Z (GMT). No. of bitstreams: 2 MICF_Tiago_Cairrao.pdf: 828306 bytes, checksum: d06679f72dabc1715b96a6f003011c64 (MD5) MICF_Tiago_Cairrao_PPT.pdf: 2079037 bytes, checksum: 2e8f9bc92a76436d27f6a7cc91f8501a (MD5) Previous issue date: 2013-10-04

Published
2013

34. Estudo epidemiológico de Candidíase invasiva na Unidade de Cuidados Intensivos do Hospital Egas Moniz-Centro Hospitalar Lisboa Ocidental

Author

Paróla, Ana Gomes, 1981, Freitas, Graciete, 1946, and Lopes, Maria Manuel Pereira, 1960

Subjects
Teses de mestrado - 2011, Susceptibilidade aos antifúngicos, Candidíase invasiva, Cuidados intensivos, Epidemiologia
Abstract

Tese de mestrado, Microbiologia Clínica, Faculdade de Medicina, Universidade de Lisboa, 2011 Submitted by Fernando João Machado (fjmachado@fm.ul.pt) on 2011-09-30T10:43:38Z No. of bitstreams: 1 620654_Tese.pdf: 1706204 bytes, checksum: db51485f7b01ea17bda666140ab6f4a7 (MD5) Made available in DSpace on 2011-09-30T10:43:49Z (GMT). No. of bitstreams: 1 620654_Tese.pdf: 1706204 bytes, checksum: db51485f7b01ea17bda666140ab6f4a7 (MD5) Previous issue date: 2010

Published
2011

35. Qualidade de amendoins consumidos em Portugal : flora fúngica e pesquisa de micotoxinas

Author

Silvério, André Ferreira Alves, 1981 and Lopes, Maria Manuel Pereira, 1960

Subjects
Micotoxinas, Cromatografia líquida de alta resolução, Portugal, Ocratoxina A, Fungos, Teses de mestrado - 2009, Aflatoxina B1
Abstract

Tese de mestrado, Microbiologia Clínica, Faculdade de Medicina, Universidade de Lisboa, 2009 Nível de preservação: critical, Criado em: 2010-01-28 17:38:28, Criado por: creator:MARTA, Alterado em: 2010-01-28 17:55:33, Modificado por: super:MARTA Made available in DSpace on 2010-07-22T08:47:49Z (GMT). No. of bitstreams: 2 21036_ulsd057607_tm2.pdf: 1477400 bytes, checksum: 4b1282433adde64a4f9d69d09649e26e (MD5) 21036.xml: 8994 bytes, checksum: 1878baaa7d912f229fa6a5d5de8c0bb2 (MD5) Previous issue date: 2009

Published
2009

36. Infecção do local cirúrgico em doentes submetidos a artroplastias no Hospital Ortopédico Dr. José de Almeida

Author

Saraiva, Raquel Cristina Borges Melo Pinto, Lopes, Maria Manuel Pereira, and Ferreira, Ana Maria Gomes

Subjects
Infecção do local cirúrgico, Profilaxia antimicrobiana cirúrgica, Factores de risco, Artroplastia primária, Teses de mestrado - 2009
Abstract

Tese de mestrado, Farmácia Hospitalar, Universidade de Lisboa, Faculdade de Farmácia, 2009 Submitted by Repositório FFUL (repositorio@ff.ul.pt) on 2014-11-11T15:02:24Z No. of bitstreams: 3 capa.pdf: 40026 bytes, checksum: 1bb0d55f53d1f2b1561a437f63a999c1 (MD5) Parte 1.pdf: 144668 bytes, checksum: edc5a6a879a312f31dea738d57f99203 (MD5) Parte 2.pdf: 1024994 bytes, checksum: 326585b5b71d56f187ea1aa91e8d81cf (MD5) Made available in DSpace on 2014-11-11T16:04:49Z (GMT). No. of bitstreams: 3 capa.pdf: 40026 bytes, checksum: 1bb0d55f53d1f2b1561a437f63a999c1 (MD5) Parte 1.pdf: 144668 bytes, checksum: edc5a6a879a312f31dea738d57f99203 (MD5) Parte 2.pdf: 1024994 bytes, checksum: 326585b5b71d56f187ea1aa91e8d81cf (MD5) Previous issue date: 2009

Published
2009

37. Avaliação do potencial de leveduras dos géneros Pseudozyma e Rhodosporidium no controlo biológico pós-colheita de bolores

Author

Alves, Maria Leonor Nobre, Sampaio, José Paulo, and Lopes, Maria Manuel

Subjects
Controle de qualidade, Alimentação, Toxicologia, Teses de mestrado
Abstract

Tese de mestrado em Controlo da Qualidade e Toxicologia dos Alimentos apresentada à Universidade de Lisboa através da Faculdade de Farmácia, 2007 Nível de preservação: critical, Criado em: 2009-11-12 20:32:04, Criado por: creator:SLOPES, Alterado em: 2009-11-12 20:46:47, Modificado por: viewer:internal Made available in DSpace on 2010-06-16T09:46:12Z (GMT). No. of bitstreams: 2 20030_ULFACD000213_TM.pdf: 2267631 bytes, checksum: 1128ccea27a7d73a886d915004b78955 (MD5) 20030.xml: 9948 bytes, checksum: 0b733213cc7ba79497941e9db1d1c04e (MD5) Previous issue date: 2007

Published
2007

38. Comparative genomic and transcriptomic analyses unveil novel features of azole resistance and adaptation to the human host in Candida glabrata.

Author

Salazar SB, Wang C, Münsterkötter M, Okamoto M, Takahashi-Nakaguchi A, Chibana H, Lopes MM, Güldener U, Butler G, and Mira NP

Subjects
Alleles, Antifungal Agents pharmacology, Computational Biology methods, Fluconazole pharmacology, Gene Deletion, Gene Expression Profiling, Gene Frequency, Genome, Fungal, Humans, Molecular Sequence Annotation, Voriconazole pharmacology, Candida glabrata drug effects, Candida glabrata physiology, Candidiasis microbiology, Drug Resistance, Fungal, Gene Expression Regulation, Fungal, Genomics methods, Host-Pathogen Interactions, Transcriptome
Abstract

The frequent emergence of azole resistance among Candida glabrata strains contributes to increase the incidence of infections caused by this species. Whole-genome sequencing of a fluconazole and voriconazole-resistant clinical isolate (FFUL887) and subsequent comparison with the genome of the susceptible strain CBS138 revealed prominent differences in several genes documented to promote azole resistance in C. glabrata. Among these was the transcriptional regulator CgPdr1. The CgPdr1 FFUL887 allele included a K274Q modification not documented in other azole-resistant strains. Transcriptomic profiling evidenced the upregulation of 92 documented targets of CgPdr1 in the FFUL887 strain, supporting the idea that the K274Q substitution originates a CgPdr1 gain-of-function mutant. The expression of CgPDR1K274Q in the FFUL887 background sensitised the cells against high concentrations of organic acids at a low pH (4.5), but had no detectable effect in tolerance towards other environmental stressors. Comparison of the genome of FFUL887 and CBS138 also revealed prominent differences in the sequence of adhesin-encoding genes, while comparison of the transcriptome of the two strains showed a significant remodelling of the expression of genes involved in metabolism of carbohydrates, nitrogen and sulphur in the FFUL887 strain; these responses likely reflecting adaptive responses evolved by the clinical strain during colonisation of the host., (© FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2018
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39. Value of morphotyping for the characterization of Candida albicans clinical isolates.

Author

Giammanco GM, Lopes MM, Coimbra RS, Pignato S, Grimont PA, Grimont F, Freitas G, and Giammanco G

Subjects
Candida albicans genetics, Candida albicans isolation & purification, DNA Fingerprinting methods, Humans, Reproducibility of Results, AIDS-Related Opportunistic Infections microbiology, Candida albicans classification, Candidiasis, Oral microbiology, Mycological Typing Techniques methods
Abstract

Until recently, morphotyping, a method evaluating fringe and surface characteristics of streak colonies grown on malt agar, has been recommended as a simple and unexpensive typing method for Candida albicans isolates. The discriminatory power and reproducibility of Hunter's modified scheme of Phongpaichit's morphotyping has been evaluated on 28 C. albicans isolates recovered from the oral cavity of asymptomatic human immunodeficiency virus-positive subjects, and compared to two molecular typing methods: randomly amplified polymorphic DNA (RAPD) fingerprinting, and contour clamped homogeneous electric field (CHEF) electrophoretic karyotyping. Morphological features of streak colonies allowed to distinguish 11 different morphotypes while RAPD fingerprinting yielded 25 different patterns and CHEF electrophoresis recognized 9 karyotypes. The discriminatory power calculated with the formula of Hunter and Gaston was 0.780 for morphotyping, 0.984 for RAPD fingerprinting, and 0.630 for karyotyping. Reproducibility was tested using 43 serial isolates from 15 subjects (2 to 6 isolates per subject) and by repeating the test after one year storage of the isolates. While genetic methods generally recognized a single type for all serial isolates from each of the subjects studied, morphotyping detected strain variations in five subjects in the absence of genetic confirmation. Poor reproducibility was demonstrated repeating morphotyping after one year storage of the isolates since differences in at least one character were detected in 92.9% of the strains.

Published
2005
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