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408 results on '"Longhi E"'

1. Anti-MAdCAM-1-Conjugated Nanocarriers Delivering Quantum Dots Enable Specific Imaging of Inflammatory Bowel Disease

Author

Truffi M, Sevieri M, Morelli L, Monieri M, Mazzucchelli S, Sorrentino L, Allevi R, Bonizzi A, Zerbi P, Marchini B, Longhi E, Sampietro GM, Colombo F, Prosperi D, Colombo M, and Corsi F

Subjects
polymeric nanoparticles, adhesion molecule, targeted imaging, Medicine (General), R5-920
Abstract

Marta Truffi,1 Marta Sevieri,2 Lucia Morelli,3 Matteo Monieri,2 Serena Mazzucchelli,2 Luca Sorrentino,2 Raffaele Allevi,2 Arianna Bonizzi,2 Pietro Zerbi,2 Beatrice Marchini,2 Erika Longhi,2 Gianluca Matteo Sampietro,4 Francesco Colombo,4 Davide Prosperi,3 Miriam Colombo,3 Fabio Corsi2,5 1Nanomedicine and Molecular Imaging Laboratory, Istituti Clinici Scientifici Maugeri IRCCS, Pavia 27100, Italy; 2Department of Biomedical and Clinical Sciences “Luigi Sacco, Università degli studi di Milano, Milano, 20157, Italy; 3NanoBioLab, Department of Biotechnology and Biosciences, Università degli studi di Milano-Bicocca, Milano, 20126, Italy; 4IBD Surgery Unit, ASST Fatebenefratelli Sacco - Ospedale “Luigi Sacco” Polo Universitario, Milano, 20157, Italy; 5Surgery Department, Breast Unit, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, 27100, ItalyCorrespondence: Fabio CorsiSurgery Department, Breast Unit, Istituti Clinici Scientifici Maugeri IRCCS, via Maugeri 4, Pavia 27100, ItalyTel +39 02 5031 9850Fax +39 02 5031 9846Email fabio.corsi@icsmaugeri.itPurpose: Assessment of inflammatory bowel disease (IBD) currently relies on aspecific clinical signs of bowel inflammation. Specific imaging of the diseased bowel regions is still lacking. Here, we investigate mucosal addressin cell adhesion molecule 1 (MAdCAM-1) as a reliable and specific endothelial target for engineered nanoparticles delivering imaging agents to obtain an exact mapping of diseased bowel foci.Materials and Methods: We generated a nanodevice composed of PLGA-PEG coupled with anti-MAdCAM-1 antibody half-chains and loaded with quantum dots (P@QD-MdC NPs). Bowel localization and systemic biodistribution of the nanoconjugate were analyzed upon injection in a murine model of chronic IBD obtained through repeated administration of dextran sulfate sodium salt. Specificity for diseased bowel regions was also assessed ex vivo in human specimens from patients with IBD. Potential for development as contrast agent in magnetic resonance imaging was assessed by preliminary study on animal model.Results: Synthesized nanoparticles revealed good stability and monodispersity. Molecular targeting properties were analyzed in vitro in a cell culture model. Upon intravenous injection, P@QD-MdC NPs were localized in the bowel of colitic mice, with enhanced accumulation at 24 h post-injection compared to untargeted nanoparticles (p< 0.05). Nanoparticles injection did not induce histologic lesions in non-target organs. Ex vivo exposure of human bowel specimens to P@QD-MdC NPs revealed specific recognition of the diseased regions vs uninvolved tracts (p< 0.0001). After loading with appropriate contrast agent, the nanoparticles enabled localized contrast enhancement of bowel mucosa in the rectum of treated mice.Conclusion: P@QD-MdC NPs efficiently detected bowel inflammation foci, accurately following the expression pattern of MAdCAM-1. Fine-tuning of this nanoconjugate with appropriate imaging agents offers a promising non-invasive tool for specific IBD diagnosis.Keywords: polymeric nanoparticles, inflamed bowel, adhesion molecule, targeted imaging

Published
2020

2. A facility for the analysis of the electronic structures of solids and their surfaces by synchrotron radiation photoelectron spectroscopy

Author

Hoesch, M., Kim, T. K., Dudin, P., Wang, H., Scott, S., Harris, P., Patel, S., Matthews, M., Hawkins, D., Alcock, S. G., Richter, T., Mudd, J. J., Basham, M., Pratt, L., Leicester, P., Longhi, E. C., Tamai, A., and Baumberger, F.

Subjects
Physics - Instrumentation and Detectors, Condensed Matter - Strongly Correlated Electrons, Condensed Matter - Superconductivity
Abstract

A synchrotron radiation beamline in the photon energy range of 18 - 240 eV and an electron spectroscopy end station have been constructed at the 3 GeV Diamond Light Source storage ring. The instrument features a variable polarisation undulator, a high resolution monochromator, a re-focussing system to form a beam spot of 50x50 micrometer^2 and an end station for angle-resolved photoelectron spectroscopy (ARPES) including a 6-degrees-of-freedom cryogenic sample manipulator. The beamline design and its performance allow for a highly productive and precise use of the ARPES technique at an energy resolution of 10 - 15 meV for fast k-space mapping studies with a photon flux up to 2 10^13 ph/sec and well below 3 meV for high resolution spectra., Comment: 10 pages, 11 figures

Published
2016
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3. Climate-related risk assessment in water safety plans: the case study of Acque Bresciane (Italy)

Author

Biasibetti, M, Longhi, E, Bozza, S, Zanotti, C, Rotiroti, M, Fumagalli, L, Caschetto, M, Redaelli, A, Bonomi, T, Biasibetti, Michela, Longhi, Elisa, Bozza, Sonia, Zanotti, Chiara, Rotiroti, Marco, Fumagalli, Letizia, Caschetto, Mariachiara, Redaelli, Agnese, Bonomi, Tullia, Biasibetti, M, Longhi, E, Bozza, S, Zanotti, C, Rotiroti, M, Fumagalli, L, Caschetto, M, Redaelli, A, Bonomi, T, Biasibetti, Michela, Longhi, Elisa, Bozza, Sonia, Zanotti, Chiara, Rotiroti, Marco, Fumagalli, Letizia, Caschetto, Mariachiara, Redaelli, Agnese, and Bonomi, Tullia

Abstract

Acque Bresciane is a public company that manages the integrated water cycle for more than 580,000 inhabitants in the Province of Brescia, in the north of Italy, providing drinking water, waste water treatment, and sewer systems. Drinking water systems are supplied with different types of groundwater, springs, and surface water sources (from lakes and rivers) whose availability and quality can be affected by climate change events. A multidisciplinary team, in collaboration with the University of Milano Bicocca, developed a specific Water Safety Plan (WSP) risk matrix focusing on the evaluation of climate-related hazardous events and calculation of their likelihood of occurrence, also using thematic maps. Moreover, to reduce the residual risks, in the risk matrix, possible control measures are suggested, such as the activation of an emergency plan, the use of other water sources, storage tanks, and interconnection with other water distribution networks. This work shows a simple and effective tool that can be applied by drinking water utilities to evaluate climate-related catchment risks, using a WSP risk matrix, thematic maps, and possible control measures to reduce risks in terms of water quality and availability and to respond with resilience to changes.

Published
2024

4. Rationale and design of the CV-PREVITAL study: an Italian multiple cohort randomised controlled trial investigating innovative digital strategies in primary cardiovascular prevention

Author

Baldassarre, D, Iacoviello, L, Baetta, R, Roncaglioni, M, Condorelli, G, Remuzzi, G, Gensini, G, Frati, L, Ricciardi, W, Conaldi, P, Uccelli, A, Blandini, F, Bosari, S, Scambia, G, Fini, M, Di Malta, A, Amato, M, Veglia, F, Bonomi, A, Klersy, C, Colazzo, F, Pengo, M, Gorini, F, Auteri, L, Ferrante, G, Baviera, M, Ambrosio, G, Catapano, A, Gialluisi, A, Malavazos, A, Castelvecchio, S, Corsi-Romanelli, M, Cardani, R, La Rovere, M, Agnese, V, Pane, B, Prati, D, Spinardi, L, Liuzzo, G, Arbustini, E, Volterrani, M, Visconti, M, Werba, J, Genovese, S, Bilo, G, Invitti, C, Di Blasio, A, Lombardi, C, Faini, A, Rosa, D, Ojeda-Fernandez, L, Foresta, A, De Curtis, A, Di Castelnuovo, A, Scalvini, S, Pierobon, A, Gorini, A, Valenti, L, Luzi, L, Racca, A, Bandi, M, Tremoli, E, Menicanti, L, Parati, G, Pompilio, G, Colombo, G, Vavassori, C, Biondi, M, Frigerio, B, Ravani, A, Sansaro, D, Coggi, D, Romandini, A, Giroli, M, Giuliani, M, Bonmi, A, Rondinelli, M, Trudu, C, Cinieri, C, Monturano, M, Colazo, F, Inviti, C, Di Blasi, A, Torlasco, C, Gilardini, L, Soranna, D, Zambon, A, Perger, E, Zanotti, L, Badano, L, Cova, L, Gentilini, D, Grappiolo, L, Condoreli, G, Ferante, G, Papa, L, Savevski, V, Ieva, F, Romano, I, Remzzi, G, Ojeda, L, Clerici, F, Palumbo, A, Genini, G, Catpano, A, Mattioli, R, Longhi, E, Mantovani, L, Madotto, F, Bonaccio, M, Gianfagna, F, Ghulam, A, Magnacca, S, Noro, F, Costanzo, S, Esposito, S, Orlandi, S, Persichillo, M, Bracone, F, Panzera, T, Ruggiero, E, Parisi, R, Franciosa, S, Morelli, M, De Rita, F, Cerletti, C, de Gaetano, G, Donati, M, Mencanti, L, Romanelli, M, Cerri, A, Dubini, C, Trevisan, M, Renna, L, Milani, V, Boveri, S, Giubbilini, P, Ramputi, L, Baroni, I, De Angeli, G, Riciardi, W, Olmetti, F, Bussotti, M, Gaetano, C, Baiardi, P, Bachetti, T, Balbi, M, Comini, L, Lorenzoni, M, Olivares, A, Traversi, E, Garre, C, Sideri, R, Clemenza, F, Gentile, G, Caruana, G, Cuscino, N, Di Gesaro, G, Greco, A, Loddo, I, Tuzzolino, F, Ucelli, A, Palombo, D, Spinella, G, Mozzetta, G, Ameri, P, Zoppoli, G, Finotello, A, Porto, I, Pratesi, G, Bladini, F, Spnardi, L, Clerici, M, Pelusi, S, Bianco, C, Carpani, R, Periti, G, Margarita, S, Lanza, G, Severino, A, Pedicino, D, D'Amario, D, D'Aiello, A, Vinci, R, Bonanni, A, Brecciaroli, M, Filomia, S, Pastorino, R, Boccia, S, Urbani, A, Sanguinetti, M, Santoliquido, A, Proto, L, Tarquini, D, Grimaldi, M, Leonardi, S, Elia, A, Currao, A, Urtis, M, Di Toro, A, Giuliani, L, Caminiti, G, Marcolongo, F, Sposato, B, Guadagni, F, Morsella, V, Marziale, A, Protti, G, Baldassarre D., Iacoviello L., Baetta R., Roncaglioni M. C., Condorelli G., Remuzzi G., Gensini G., Frati L., Ricciardi W., Conaldi P. G., Uccelli A., Blandini F., Bosari S., Scambia G., Fini M., Di Malta A., Amato M., Veglia F., Bonomi A., Klersy C., Colazzo F., Pengo M., Gorini F., Auteri L., Ferrante G., Baviera M., Ambrosio G., Catapano A., Gialluisi A., Malavazos A. E., Castelvecchio S., Corsi-Romanelli M. M., Cardani R., La Rovere M. T., Agnese V., Pane B., Prati D., Spinardi L., Liuzzo G., Arbustini E., Volterrani M., Visconti M., Werba J. P., Genovese S., Bilo G., Invitti C., Di Blasio A., Lombardi C., Faini A., Rosa D., Ojeda-Fernandez L., Foresta A., De Curtis A., Di Castelnuovo A., Scalvini S., Pierobon A., Gorini A., Valenti L., Luzi L., Racca A., Bandi M., Tremoli E., Menicanti L., Parati G., Pompilio G., Colombo G., Vavassori C., Biondi M. L., Frigerio B., Ravani A., Sansaro D., Coggi D., Romandini A., Giroli M., Giuliani M., Bonmi A., Rondinelli M., Trudu C., Cinieri C., Monturano M., Colazo F., Inviti C., Di Blasi A., Torlasco C., Gilardini L., Soranna D., Zambon A., Perger E., Zanotti L., Badano L., Cova L., Gentilini D., Grappiolo L., Condoreli G., Ferante G., Papa L., Savevski V., Ieva F., Romano I., Remzzi G., Ojeda L., Clerici F., Palumbo A., Genini G. F., Catpano A., Mattioli R., Longhi E., Mantovani L. G., Madotto F., Bonaccio M., Gianfagna F., Ghulam A., Magnacca S., Noro F., Costanzo S., Esposito S., Orlandi S., Persichillo M., Bracone F., Panzera T., Ruggiero E., Parisi R., Franciosa S., Morelli M., De Rita F., Cerletti C., de Gaetano G., Donati M. B., Mencanti L., Romanelli M. M. C., Cerri A., Dubini C., Trevisan M. B., Renna L. V., Milani V., Boveri S., Giubbilini P., Ramputi L., Baroni I., De Angeli G., Riciardi W., Olmetti F., Bussotti M., Gaetano C., Baiardi P., Bachetti T., Balbi M., Comini L., Lorenzoni M., Olivares A., Traversi E., Garre C., Sideri R., Clemenza F., Gentile G., Caruana G., Cuscino N., Di Gesaro G., Greco A., Loddo I., Tuzzolino F., Ucelli A., Palombo D., Spinella G., Mozzetta G., Ameri P., Zoppoli G., Finotello A., Porto I., Pratesi G., Bladini F., Spnardi L., Clerici M., Pelusi S., Bianco C., Carpani R., Periti G., Margarita S., Lanza G. A., Severino A., Pedicino D., D'Amario D., D'Aiello A., Vinci R., Bonanni A., Brecciaroli M., Filomia S., Pastorino R., Boccia S., Urbani A., Sanguinetti M., Santoliquido A., Proto L., Tarquini D., Grimaldi M. C., Leonardi S., Elia A., Currao A., Urtis M., Di Toro A., Giuliani L., Caminiti G., Marcolongo F., Sposato B., Guadagni F., Morsella V., Marziale A., Protti G., Baldassarre, D, Iacoviello, L, Baetta, R, Roncaglioni, M, Condorelli, G, Remuzzi, G, Gensini, G, Frati, L, Ricciardi, W, Conaldi, P, Uccelli, A, Blandini, F, Bosari, S, Scambia, G, Fini, M, Di Malta, A, Amato, M, Veglia, F, Bonomi, A, Klersy, C, Colazzo, F, Pengo, M, Gorini, F, Auteri, L, Ferrante, G, Baviera, M, Ambrosio, G, Catapano, A, Gialluisi, A, Malavazos, A, Castelvecchio, S, Corsi-Romanelli, M, Cardani, R, La Rovere, M, Agnese, V, Pane, B, Prati, D, Spinardi, L, Liuzzo, G, Arbustini, E, Volterrani, M, Visconti, M, Werba, J, Genovese, S, Bilo, G, Invitti, C, Di Blasio, A, Lombardi, C, Faini, A, Rosa, D, Ojeda-Fernandez, L, Foresta, A, De Curtis, A, Di Castelnuovo, A, Scalvini, S, Pierobon, A, Gorini, A, Valenti, L, Luzi, L, Racca, A, Bandi, M, Tremoli, E, Menicanti, L, Parati, G, Pompilio, G, Colombo, G, Vavassori, C, Biondi, M, Frigerio, B, Ravani, A, Sansaro, D, Coggi, D, Romandini, A, Giroli, M, Giuliani, M, Bonmi, A, Rondinelli, M, Trudu, C, Cinieri, C, Monturano, M, Colazo, F, Inviti, C, Di Blasi, A, Torlasco, C, Gilardini, L, Soranna, D, Zambon, A, Perger, E, Zanotti, L, Badano, L, Cova, L, Gentilini, D, Grappiolo, L, Condoreli, G, Ferante, G, Papa, L, Savevski, V, Ieva, F, Romano, I, Remzzi, G, Ojeda, L, Clerici, F, Palumbo, A, Genini, G, Catpano, A, Mattioli, R, Longhi, E, Mantovani, L, Madotto, F, Bonaccio, M, Gianfagna, F, Ghulam, A, Magnacca, S, Noro, F, Costanzo, S, Esposito, S, Orlandi, S, Persichillo, M, Bracone, F, Panzera, T, Ruggiero, E, Parisi, R, Franciosa, S, Morelli, M, De Rita, F, Cerletti, C, de Gaetano, G, Donati, M, Mencanti, L, Romanelli, M, Cerri, A, Dubini, C, Trevisan, M, Renna, L, Milani, V, Boveri, S, Giubbilini, P, Ramputi, L, Baroni, I, De Angeli, G, Riciardi, W, Olmetti, F, Bussotti, M, Gaetano, C, Baiardi, P, Bachetti, T, Balbi, M, Comini, L, Lorenzoni, M, Olivares, A, Traversi, E, Garre, C, Sideri, R, Clemenza, F, Gentile, G, Caruana, G, Cuscino, N, Di Gesaro, G, Greco, A, Loddo, I, Tuzzolino, F, Ucelli, A, Palombo, D, Spinella, G, Mozzetta, G, Ameri, P, Zoppoli, G, Finotello, A, Porto, I, Pratesi, G, Bladini, F, Spnardi, L, Clerici, M, Pelusi, S, Bianco, C, Carpani, R, Periti, G, Margarita, S, Lanza, G, Severino, A, Pedicino, D, D'Amario, D, D'Aiello, A, Vinci, R, Bonanni, A, Brecciaroli, M, Filomia, S, Pastorino, R, Boccia, S, Urbani, A, Sanguinetti, M, Santoliquido, A, Proto, L, Tarquini, D, Grimaldi, M, Leonardi, S, Elia, A, Currao, A, Urtis, M, Di Toro, A, Giuliani, L, Caminiti, G, Marcolongo, F, Sposato, B, Guadagni, F, Morsella, V, Marziale, A, Protti, G, Baldassarre D., Iacoviello L., Baetta R., Roncaglioni M. C., Condorelli G., Remuzzi G., Gensini G., Frati L., Ricciardi W., Conaldi P. G., Uccelli A., Blandini F., Bosari S., Scambia G., Fini M., Di Malta A., Amato M., Veglia F., Bonomi A., Klersy C., Colazzo F., Pengo M., Gorini F., Auteri L., Ferrante G., Baviera M., Ambrosio G., Catapano A., Gialluisi A., Malavazos A. E., Castelvecchio S., Corsi-Romanelli M. M., Cardani R., La Rovere M. T., Agnese V., Pane B., Prati D., Spinardi L., Liuzzo G., Arbustini E., Volterrani M., Visconti M., Werba J. P., Genovese S., Bilo G., Invitti C., Di Blasio A., Lombardi C., Faini A., Rosa D., Ojeda-Fernandez L., Foresta A., De Curtis A., Di Castelnuovo A., Scalvini S., Pierobon A., Gorini A., Valenti L., Luzi L., Racca A., Bandi M., Tremoli E., Menicanti L., Parati G., Pompilio G., Colombo G., Vavassori C., Biondi M. L., Frigerio B., Ravani A., Sansaro D., Coggi D., Romandini A., Giroli M., Giuliani M., Bonmi A., Rondinelli M., Trudu C., Cinieri C., Monturano M., Colazo F., Inviti C., Di Blasi A., Torlasco C., Gilardini L., Soranna D., Zambon A., Perger E., Zanotti L., Badano L., Cova L., Gentilini D., Grappiolo L., Condoreli G., Ferante G., Papa L., Savevski V., Ieva F., Romano I., Remzzi G., Ojeda L., Clerici F., Palumbo A., Genini G. F., Catpano A., Mattioli R., Longhi E., Mantovani L. G., Madotto F., Bonaccio M., Gianfagna F., Ghulam A., Magnacca S., Noro F., Costanzo S., Esposito S., Orlandi S., Persichillo M., Bracone F., Panzera T., Ruggiero E., Parisi R., Franciosa S., Morelli M., De Rita F., Cerletti C., de Gaetano G., Donati M. B., Mencanti L., Romanelli M. M. C., Cerri A., Dubini C., Trevisan M. B., Renna L. V., Milani V., Boveri S., Giubbilini P., Ramputi L., Baroni I., De Angeli G., Riciardi W., Olmetti F., Bussotti M., Gaetano C., Baiardi P., Bachetti T., Balbi M., Comini L., Lorenzoni M., Olivares A., Traversi E., Garre C., Sideri R., Clemenza F., Gentile G., Caruana G., Cuscino N., Di Gesaro G., Greco A., Loddo I., Tuzzolino F., Ucelli A., Palombo D., Spinella G., Mozzetta G., Ameri P., Zoppoli G., Finotello A., Porto I., Pratesi G., Bladini F., Spnardi L., Clerici M., Pelusi S., Bianco C., Carpani R., Periti G., Margarita S., Lanza G. A., Severino A., Pedicino D., D'Amario D., D'Aiello A., Vinci R., Bonanni A., Brecciaroli M., Filomia S., Pastorino R., Boccia S., Urbani A., Sanguinetti M., Santoliquido A., Proto L., Tarquini D., Grimaldi M. C., Leonardi S., Elia A., Currao A., Urtis M., Di Toro A., Giuliani L., Caminiti G., Marcolongo F., Sposato B., Guadagni F., Morsella V., Marziale A., and Protti G.

Abstract

Introduction Prevention of cardiovascular disease (CVD) is of key importance in reducing morbidity, disability and mortality worldwide. Observational studies suggest that digital health interventions can be an effective strategy to reduce cardiovascular (CV) risk. However, evidence from large randomised clinical trials is lacking. Methods and analysis The CV-PREVITAL study is a multicentre, prospective, randomised, controlled, open-label interventional trial designed to compare the effectiveness of an educational and motivational mobile health (mHealth) intervention versus usual care in reducing CV risk. The intervention aims at improving diet, physical activity, sleep quality, psycho-behavioural aspects, as well as promoting smoking cessation and adherence to pharmacological treatment for CV risk factors. The trial aims to enrol approximately 80 000 subjects without overt CVDs referring to general practitioners' offices, community pharmacies or clinics of Scientific Institute for Research, Hospitalization and Health Care (Italian acronym IRCCS) affiliated with the Italian Cardiology Network. All participants are evaluated at baseline and after 12 months to assess the effectiveness of the intervention on short-term endpoints, namely improvement in CV risk score and reduction of major CV risk factors. Beyond the funded life of the study, a long-term (7 years) follow-up is also planned to assess the effectiveness of the intervention on the incidence of major adverse CV events. A series of ancillary studies designed to evaluate the effect of the mHealth intervention on additional risk biomarkers are also performed. Ethics and dissemination This study received ethics approval from the ethics committee of the coordinating centre (Monzino Cardiology Center; R1256/20-CCM 1319) and from all other relevant IRBs and ethics committees. Findings are disseminated through scientific meetings and peer-reviewed journals and via social media. Partners are informed about the study's

Published
2023

5. Fluctuations in granular materials

Author

Behringer, R. P., primary, Geng, Junfei, additional, Howell, D., additional, Longhi, E., additional, Reydellet, G., additional, Vanel, L., additional, Clément, E., additional, and Luding, S., additional

Published
2020
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6. Footprints in Sand: The Response of a Granular Material to Local Perturbations

Author

Geng, Junfei, Howell, D., Longhi, E., Behringer, R. P., Reydellet, G., Vanel, L., Clement, E., and Luding, S.

Subjects
Condensed Matter - Materials Science
Abstract

We experimentally determine ensemble-averaged responses of granular packings to point forces, and we compare these results to recent models for force propagation in a granular material. We used 2D granular arrays consisting of photoelastic particles: either disks or pentagons, thus spanning the range from ordered to disordered packings. A key finding is that spatial ordering of the particles is a key factor in the force response. Ordered packings have a propagative component that does not occur in disordered packings., Comment: 5 pages, 4 eps figures, Phys. Rev. Lett. 87, 035506 (2001)

Published
2000
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8. Work function and energy level alignment tuning at Ti3C2Tx MXene surfaces and interfaces using metal organic donor acceptor molecules

Author

Schultz, T., Bärmann, P., Longhi, E., Meena, R., Geerts, Y., Gogotsi, Y., Barlow, S., Marder, S., Petit, T., and Koch, N.

Subjects
no topic specified
Abstract

Two dimensional MXenes, with Ti3C2Tx being the most prominent member, show properties that make them promising for a manifold of applications, including electrodes in light emitting diodes, solar cells, and field effect transistors based on organic semiconductors. In these cases, the work function of MXenes plays an important role in the energy level alignment to the subsequently deposited organic layer, as it determines the electron and hole injection barriers. Therefore, methods for controlling the Ti3C2Tx work function should be developed. We demonstrate that, by using thin layers of metal organic donor acceptor molecules, the work function of Ti3C2Tx can be tuned over a range of gt;3 eV. This enables tuning the energy level alignment to a subsequently deposited organic semiconductor, all the way from intrinsic Fermi level pinning at the highest occupied molecular energy level minimal hole injection barrier to pinning at the lowest unoccupied level minimal electron injection barrier . Furthermore, it is shown that a predominantly oxygen terminated surface does not lead to an extraordinary high work function, in contrast to theoretical predictions. The proposed strategy may greatly expand the use of MXenes in conjunction with organic hole and electron transport layers in optoelectronic devices

Published
2023

9. Occupational exposure in the lombardy region (Italy) to sars-cov-2 infection: Results from the mustang–occupation–covid-19 study

Author

Valle, P, Fabbri, M, Madotto, F, Ferrara, P, Cozzolino, P, Calabretto, E, D'Orso, M, Longhi, E, Polosa, R, Riva, M, Mazzaglia, G, Sommese, C, Mantovani, L, Agazzi, G, Anzaldi, E, Berardi, C, Beretta, P, Clemente, F, Crotti, G, Harari, S, Lorelli, S, Mancino, M, Pedretti, R, Polizzotti, G, Raucci, P, Spatafora, C, Squassabia, G, Staine, T, Zuccato, C, Valle P. D., Fabbri M., Madotto F., Ferrara P., Cozzolino P., Calabretto E., D'orso M. I., Longhi E., Polosa R., Riva M. A., Mazzaglia G., Sommese C., Mantovani L. G., Agazzi G., Anzaldi E., Berardi C., Beretta P., Clemente F., Crotti G., Harari S., Lorelli S., Mancino M., Pedretti R. F. E., Polizzotti G., Raucci P., Spatafora C., Squassabia G., Staine T., Zuccato C., Valle, P, Fabbri, M, Madotto, F, Ferrara, P, Cozzolino, P, Calabretto, E, D'Orso, M, Longhi, E, Polosa, R, Riva, M, Mazzaglia, G, Sommese, C, Mantovani, L, Agazzi, G, Anzaldi, E, Berardi, C, Beretta, P, Clemente, F, Crotti, G, Harari, S, Lorelli, S, Mancino, M, Pedretti, R, Polizzotti, G, Raucci, P, Spatafora, C, Squassabia, G, Staine, T, Zuccato, C, Valle P. D., Fabbri M., Madotto F., Ferrara P., Cozzolino P., Calabretto E., D'orso M. I., Longhi E., Polosa R., Riva M. A., Mazzaglia G., Sommese C., Mantovani L. G., Agazzi G., Anzaldi E., Berardi C., Beretta P., Clemente F., Crotti G., Harari S., Lorelli S., Mancino M., Pedretti R. F. E., Polizzotti G., Raucci P., Spatafora C., Squassabia G., Staine T., and Zuccato C.

Abstract

Sero-epidemiological surveys are valuable attempts to estimate the circulation of SARS-CoV-2 in general or selected populations. Within this context, a prospective observational study was conducted to estimate the prevalence and persistence of SARS-CoV-2 antibodies in different categories of workers and factors associated with positivity, through the detection of virus-specific immunoglobulin G and M (IgG/IgM) in serum samples. Enrollees were divided in low exposure and medium-high groups on the basis of their work activity. Antibody responders were re-contacted after 3 months for the follow-up. Of 2255 sampled workers, 4.8% tested positive for SARS-CoV-2 IgG/IgM antibodies, with 81.7% to IgG only. Workers who continued to go to their place of work, were healthcare workers, or experienced at least one COVID-19-related symptom were more likely to test positive for SARS-CoV-2 antibodies. SARS-CoV-2 antibodies prevalence was significantly higher in the medium-high risk vs. low-risk group (7.2% vs. 3.0%, p < 0.0001). At 3-month follow-up, 81.3% of subjects still had antibody response. This study provided important information of SARS-CoV-2 infection prevalence among workers in northern Italy, where the impact of COVID-19 was particularly intense. The presented surveillance data give a contribution to refine current estimates of the disease burden expected from the SARS-CoV-2.

Published
2021

10. Anti-madcam-1-conjugated nanocarriers delivering quantum dots enable specific imaging of inflammatory bowel disease

Author

Truffi, M, Sevieri, M, Morelli, L, Monieri, M, Mazzucchelli, S, Sorrentino, L, Allevi, R, Bonizzi, A, Zerbi, P, Marchini, B, Longhi, E, Sampietro, G, Colombo, F, Prosperi, D, Colombo, M, Corsi, F, Truffi M., Sevieri M., Morelli L., Monieri M., Mazzucchelli S., Sorrentino L., Allevi R., Bonizzi A., Zerbi P., Marchini B., Longhi E., Sampietro G. M., Colombo F., Prosperi D., Colombo M., Corsi F., Truffi, M, Sevieri, M, Morelli, L, Monieri, M, Mazzucchelli, S, Sorrentino, L, Allevi, R, Bonizzi, A, Zerbi, P, Marchini, B, Longhi, E, Sampietro, G, Colombo, F, Prosperi, D, Colombo, M, Corsi, F, Truffi M., Sevieri M., Morelli L., Monieri M., Mazzucchelli S., Sorrentino L., Allevi R., Bonizzi A., Zerbi P., Marchini B., Longhi E., Sampietro G. M., Colombo F., Prosperi D., Colombo M., and Corsi F.

Abstract

Purpose: Assessment of inflammatory bowel disease (IBD) currently relies on aspecific clinical signs of bowel inflammation. Specific imaging of the diseased bowel regions is still lacking. Here, we investigate mucosal addressin cell adhesion molecule 1 (MAdCAM-1) as a reliable and specific endothelial target for engineered nanoparticles delivering imaging agents to obtain an exact mapping of diseased bowel foci. Materials and Methods: We generated a nanodevice composed of PLGA-PEG coupled with anti-MAdCAM-1 antibody half-chains and loaded with quantum dots (P@QD-MdC NPs). Bowel localization and systemic biodistribution of the nanoconjugate were analyzed upon injection in a murine model of chronic IBD obtained through repeated administration of dextran sulfate sodium salt. Specificity for diseased bowel regions was also assessed ex vivo in human specimens from patients with IBD. Potential for development as contrast agent in magnetic resonance imaging was assessed by preliminary study on animal model. Results: Synthesized nanoparticles revealed good stability and monodispersity. Molecular targeting properties were analyzed in vitro in a cell culture model. Upon intravenous injection, P@QD-MdC NPs were localized in the bowel of colitic mice, with enhanced accumulation at 24 h post-injection compared to untargeted nanoparticles (p<0.05). Nanoparticles injection did not induce histologic lesions in non-target organs. Ex vivo exposure of human bowel specimens to P@QD-MdC NPs revealed specific recognition of the diseased regions vs uninvolved tracts (p<0.0001). After loading with appropriate contrast agent, the nanoparticles enabled localized contrast enhancement of bowel mucosa in the rectum of treated mice. Conclusion: P@QD-MdC NPs efficiently detected bowel inflammation foci, accurately following the expression pattern of MAdCAM-1. Fine-tuning of this nanoconjugate with appropriate imaging agents offers a promising non-invasive tool for specific IBD diagnosis.

Published
2020

11. Co-administration of H-ferritin-doxorubicin and Trastuzumab in neoadjuvant setting improves efficacy and prevents cardiotoxicity in HER2 + murine breast cancer model

Author

Andreata, F, Bonizzi, A, Sevieri, M, Truffi, M, Monieri, M, Sitia, L, Silva, F, Sorrentino, L, Allevi, R, Zerbi, P, Marchini, B, Longhi, E, Ottria, R, Casati, S, Vanna, R, Morasso, C, Bellini, M, Prosperi, D, Corsi, F, Mazzucchelli, S, Andreata F., Bonizzi A., Sevieri M., Truffi M., Monieri M., Sitia L., Silva F., Sorrentino L., Allevi R., Zerbi P., Marchini B., Longhi E., Ottria R., Casati S., Vanna R., Morasso C., Bellini M., Prosperi D., Corsi F., Mazzucchelli S., Andreata, F, Bonizzi, A, Sevieri, M, Truffi, M, Monieri, M, Sitia, L, Silva, F, Sorrentino, L, Allevi, R, Zerbi, P, Marchini, B, Longhi, E, Ottria, R, Casati, S, Vanna, R, Morasso, C, Bellini, M, Prosperi, D, Corsi, F, Mazzucchelli, S, Andreata F., Bonizzi A., Sevieri M., Truffi M., Monieri M., Sitia L., Silva F., Sorrentino L., Allevi R., Zerbi P., Marchini B., Longhi E., Ottria R., Casati S., Vanna R., Morasso C., Bellini M., Prosperi D., Corsi F., and Mazzucchelli S.

Abstract

Neoadjuvant chemotherapy has been established as the standard of care for HER2-positive breast cancer since it allows cancer down-staging, up to pathological complete response. The standard of care in the neoadjuvant setting for HER2-positive breast cancer is a combination of highly cytotoxic drugs such as anthracyclines and the anti-HER2 monoclonal antibody. Despite this cocktail allows a pathological complete response in up to 50%, their co-administration is strongly limited by intrinsic cardiotoxicity. Therefore, only a sequential administration of anthracyclines and the anti-HER2 treatment is allowed. Here, we propose the anthracycline formulation in H-Ferritin nanocages as promising candidate to solve this unmet clinical need, thanks to its capability to increase anthracyclines efficacy while reducing their cardiotoxicity. Treating a murine model of HER2-positive breast cancer with co-administration of Trastuzumab and H-Ferritin anthracycline nanoformulation, we demonstrate an improved tumor penetration of drugs, leading to increased anticancer efficacy and reduced of cardiotoxicity.

Published
2020

12. SARS-CoV-2 Immunization Orchestrates the Amplification of IFNγ-Producing T Cell and NK Cell Persistence

Author

La Sala, L., Gandini, S., Bruno, A., Allevi, R., Gallazzi, M., Senesi, P., Palano, M. T., Meregalli, P., Longhi, E., Sommese, C., Luzi, L., and Trabucchi, E.

Subjects
Adult, Male, B-Lymphocytes, NK, CD4, CD8, immune response, SARS-CoV-2, vaccines, BNT162 Vaccine, COVID-19, Female, Humans, Interferon-gamma, Interleukin-4, Killer Cells, Natural, Leukocytes, Mononuclear, Lymphocyte Subsets, T-Lymphocytes, Th1-Th2 Balance, Immunization, Mononuclear, Natural, Leukocytes, Killer Cells
Published
2022

13. Occupational Exposure in the Lombardy Region (Italy) to SARS-CoV-2 Infection: Results from the MUSTANG–OCCUPATION–COVID-19 Study

Author

Valle P. D., Fabbri M., Madotto F., Ferrara P., Cozzolino P., Calabretto E., D'orso M. I., Longhi E., Polosa R., Riva M. A., Mazzaglia G., Sommese C., Mantovani L. G., Agazzi G., Anzaldi E., Berardi C., Beretta P., Clemente F., Crotti G., Harari S., Lorelli S., Mancino M., Pedretti R. F. E., Polizzotti G., Raucci P., Spatafora C., Squassabia G., Staine T., Zuccato C., Valle, P, Fabbri, M, Madotto, F, Ferrara, P, Cozzolino, P, Calabretto, E, D'Orso, M, Longhi, E, Polosa, R, Riva, M, Mazzaglia, G, Sommese, C, Mantovani, L, Agazzi, G, Anzaldi, E, Berardi, C, Beretta, P, Clemente, F, Crotti, G, Harari, S, Lorelli, S, Mancino, M, Pedretti, R, Polizzotti, G, Raucci, P, Spatafora, C, Squassabia, G, Staine, T, and Zuccato, C

Subjects
Health, Toxicology and Mutagenesis, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), lcsh:Medicine, Context (language use), 030204 cardiovascular system & hematology, Antibodies, Viral, Article, Immunoglobulin G, Persistence (computer science), 03 medical and health sciences, 0302 clinical medicine, Humans, Medicine, Seroprevalence, 030212 general & internal medicine, Occupations, antibody persistence, Disease burden, biology, seroprevalence, business.industry, SARS-CoV-2, lcsh:R, Public Health, Environmental and Occupational Health, COVID-19, occupational exposure, Italy, biology.protein, Observational study, Antibody, business, Demography
Abstract

Sero-epidemiological surveys are valuable attempts to estimate the circulation of SARS-CoV-2 in general or selected populations. Within this context, a prospective observational study was conducted to estimate the prevalence and persistence of SARS-CoV-2 antibodies in different categories of workers and factors associated with positivity, through the detection of virus-specific immunoglobulin G and M (IgG/IgM) in serum samples. Enrollees were divided in low exposure and medium-high groups on the basis of their work activity. Antibody responders were re-contacted after 3 months for the follow-up. Of 2255 sampled workers, 4.8% tested positive for SARS-CoV-2 IgG/IgM antibodies, with 81.7% to IgG only. Workers who continued to go to their place of work, were healthcare workers, or experienced at least one COVID-19-related symptom were more likely to test positive for SARS-CoV-2 antibodies. SARS-CoV-2 antibodies prevalence was significantly higher in the medium-high risk vs. low-risk group (7.2% vs. 3.0%, p &lt, 0.0001). At 3-month follow-up, 81.3% of subjects still had antibody response. This study provided important information of SARS-CoV-2 infection prevalence among workers in northern Italy, where the impact of COVID-19 was particularly intense. The presented surveillance data give a contribution to refine current estimates of the disease burden expected from the SARS-CoV-2.

Published
2021
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14. Use of DPB1 T-cell epitope algorithm among italian transplant centers: A survey on behalf of Associazione Italiana di Immunogenetica e Biologia dei Trapianti

Author

Crocchiolo, R., Mele, L., Testi, M., Scollo Chiara, M., Murgia, B., Rossi, A., Vecchiato, C., Grammatico, P., Mininni, D., Longhi, E., Manfroi, S., Giuliodori, S., Castellani, L., Carella, G., Lai, S., Azzaro Maria, P., Mazzi, B., Perotti, L., Penta, R., Lombardo, C., Tognellini, R., Andreani, M., Albergoni Maria, P., Nesci, S., Cappuzzo, V., Chiusolo, Patrizia, Garino, E., Cappucci, G., Ceschini, N., Bevilacqua, E., Guizzardi, E., Tagliaferri Cinzia, M., Piazza, A., Carcassi, C., Miotti, V., Chiusolo P. (ORCID:0000-0002-1355-1587), Crocchiolo, R., Mele, L., Testi, M., Scollo Chiara, M., Murgia, B., Rossi, A., Vecchiato, C., Grammatico, P., Mininni, D., Longhi, E., Manfroi, S., Giuliodori, S., Castellani, L., Carella, G., Lai, S., Azzaro Maria, P., Mazzi, B., Perotti, L., Penta, R., Lombardo, C., Tognellini, R., Andreani, M., Albergoni Maria, P., Nesci, S., Cappuzzo, V., Chiusolo, Patrizia, Garino, E., Cappucci, G., Ceschini, N., Bevilacqua, E., Guizzardi, E., Tagliaferri Cinzia, M., Piazza, A., Carcassi, C., Miotti, V., and Chiusolo P. (ORCID:0000-0002-1355-1587)

Abstract

The HLA-DPB1 locus has been demonstrated to have a significant role on patients' outcome after allogeneic HSCT, and the so-called T-cell epitope (TCE) algorithm has been incorporated in international guidelines for the selection of unrelated donors. The purpose of the present study is to measure, through a national survey conducted on behalf of the Associazione Italiana di Immunogenetica e Biologia dei Trapianti (AIBT), the extent of awareness and use of HLA-DPB1 TCE-based algorithms during the donor search. 89% of the HLA laboratories answered to a short questionnaire and the results showed a progressive increase of the laboratories typing DPB1 in patients and their potential donors during the search (from 44% to 79% during the 2010–2019 period) as well as the application of a TCE-based algorithm for the donor choice whenever possible (from 24% to 65% during the same period). The DP-permissiveness status is detailed in the official HLA typing report by 12%, 32% and 50% of laboratories in 2010, 2015 and 2019, respectively. The present data indicate an encouraging raise in the awareness of the HLA-DPB1 role in unrelated donor selection; noteworthy, mentioning the TCE-based permissiveness status in the HLA typing report of each potential unrelated donor represents a notable mean to raise awareness among transplant physicians and to support them in their task of choosing the best donor. Nonetheless, despite the compelling evidence of the predictive ability of TCE-based algorithms, further efforts are still needed to extend its application to all transplant centers in Italy.

Published
2021

19. MAdCAM-1 nanotargeting uncovers bowel inflammation foci in experimental model of colitis

Author

Truffi, M, Colombo, M, Sorrentino, L, Mazzucchelli, S, Monieri, M, Longhi, E, Prosperi, D, Corsi, F, Truffi M., Colombo M., Sorrentino L., Mazzucchelli S., Monieri M., Longhi E., Prosperi D., Corsi F., Truffi, M, Colombo, M, Sorrentino, L, Mazzucchelli, S, Monieri, M, Longhi, E, Prosperi, D, Corsi, F, Truffi M., Colombo M., Sorrentino L., Mazzucchelli S., Monieri M., Longhi E., Prosperi D., and Corsi F.

Abstract

Evaluation of acute colitis relies on invasive endoscopic examination to assess the presence and the severity of the disease, and on aspecific radiological signs of bowel inflammation, such as contrast enhancement of bowel wall thickening. Consequently, the current best clinical management only offers an approximation of the real clinical scenario, and has a negative impact on the quality of life of patients. An accurate stadiation of inflammatory bowel diseases for assessment of response to therapies or indication for surgery remains challenging. Here, mucosal addressin cell-adhesion molecule-1 (MAdCAM-1) was investigated as a reliable and specific target of active bowel inflammation, to be exploited for site-specific nanotheranostics in a preclinical murine model of colitis. MAdCAM-1 is a cell adhesion molecule, which is upregulated on gut endothelium in case of inflammation at very early stages of the disease, and is responsible for T cells recruitment in involved bowel tracts. Moreover, it is finely related to activity of inflammatory bowel diseases and correlates with response to therapy. Anti-MAdCAM-1 antibodies were conjugated to the surface of smart and safe delivery nanoparticles, consisting of a manganese oxide core endowed with paramagnetic properties. The aim of the study was to assess the in vivo targeting efficacy and the accuracy of these innovative low-toxic MAdCAM-1-targeted nanoparticles, specifically designed to detect active bowel inflammation foci at early stages of the disease. Manganese oxide nanoparticles revealed good stability and polydispersity. Their negligible citotoxicity was demonstrated on endothelial cell cultures by analysis of viability and apoptosis, and on blood samples by hemolysis assay. Colitis was induced in mice by oral treatment with dextran sulfate sodium, and MAdCAM-1 overexpression was assessed on the intestinal vascular endothelium by immunohistochemistry and western blotting. Fluorescent anti-MAdCAM-1 nanoparticles wer

Published
2018

47. Abstract P1-20-04: Nanoformulation of doxorubicin inside H- ferritin nanocages allows a cardio-safe combined therapy with trastuzumab: De-escalating cardiotoxicity in HER2-positive breast cancer

Author

Mazzucchelli, S, primary, Andreata, F, additional, Bonizzi, A, additional, Monieri, M, additional, Bellini, M, additional, Longhi, E, additional, Ottria, R, additional, Sorrentino, L, additional, Truffi, M, additional, Prosperi, D, additional, Zerbi, P, additional, and Corsi, F, additional

Published
2019
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48. Dynamic facial expressions of emotions are discriminated at birth

Author

Addabbo, M, Longhi, E, Marchis, I, Tagliabue, P, Turati, C, Turati, C., Addabbo, M, Longhi, E, Marchis, I, Tagliabue, P, Turati, C, and Turati, C.

Abstract

The ability to discriminate between different facial expressions is fundamental since the first stages of postnatal life. The aim of this study is to investigate whether 2-days-old newborns are capable to discriminate facial expressions of emotions as they naturally take place in everyday interactions, that is in motion. When two dynamic displays depicting a happy and a disgusted facial expression were simultaneously presented (i.e., visual preference paradigm), newborns did not manifest any visual preference (Experiment 1). Nonetheless, after being habituated to a happy or disgusted dynamic emotional expression (i.e., habituation paradigm), newborns successfully discriminated between the two (Experiment 2). These results indicate that at birth newborns are sensitive to dynamic faces expressing emotions.

Published
2018

49. Nano-targeting of Mucosal Addressin Cell Adhesion Molecule-1 identifies bowel inflammation foci in murine model

Author

Truffi, M, Colombo, M, Peñaranda Avila, J, Sorrentino, L, Monieri, M, Collico, V, Zerbi, P, Longhi, E, Allevi, R, Prosperi, D, Corsi, F, Corsi, F., COLOMBO, MIRIAM, COLLICO, VERONICA, PROSPERI, DAVIDE, Truffi, M, Colombo, M, Peñaranda Avila, J, Sorrentino, L, Monieri, M, Collico, V, Zerbi, P, Longhi, E, Allevi, R, Prosperi, D, Corsi, F, Corsi, F., COLOMBO, MIRIAM, COLLICO, VERONICA, and PROSPERI, DAVIDE

Abstract

Aim: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics. Materials & methods: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology. Results: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs. Conclusion: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

Published
2017

50. Nanometronomic treatment of 4T1 breast cancer with nanocaged doxorubicin prevents drug resistance and circumvents cardiotoxicity

Author

Mazzucchelli, S, Bellini, M, Fiandra, L, Truffi, M, Rizzuto, M, Sorrentino, L, Longhi, E, Nebuloni, M, Prosperi, D, Corsi, F, BELLINI, MICHELA, FIANDRA, LUISA, RIZZUTO, MARIA ANTONIETTA, PROSPERI, DAVIDE, Corsi, F., Mazzucchelli, S, Bellini, M, Fiandra, L, Truffi, M, Rizzuto, M, Sorrentino, L, Longhi, E, Nebuloni, M, Prosperi, D, Corsi, F, BELLINI, MICHELA, FIANDRA, LUISA, RIZZUTO, MARIA ANTONIETTA, PROSPERI, DAVIDE, and Corsi, F.

Abstract

Chemotherapeutic treatment of breast cancer is based on maximum tolerated dose (MTD) approach. However, advanced stage tumors are not effectively eradicated by MTD owing to suboptimal drug targeting, onset of therapeutic resistance and neoangiogenesis. In contrast, "metronomic" chemotherapy is based on frequent drug administrations at lower doses, resulting in neovascularization inhibition and induction of tumor dormancy. Here we show the potential of H-ferritin (HFn)-mediated targeted nanodelivery of metronomic doxorubicin (DOX) in the setting of a highly aggressive and metastatic 4T1 breast cancer mouse model with DOX-inducible expression of chemoresistance. We find that HFn-DOX administered at repeated doses of 1.24 mg kg-1 strongly improves the antitumor potential of DOX chemotherapy arresting the tumor progression. We find that such a potent antitumor effect is attributable to multiple nanodrug actions beyond cell killing, including inhibition of tumor angiogenesis and avoidance of chemoresistance. Multiparametric assessment of heart tissues, including histology, ultrastructural analysis of tissue morphology, and measurement of markers of reactive oxygen species and hepatic/renal conditions, provided evidence that metronomic HFn-DOX allowed us to overcome cardiotoxicity. Our results suggest that HFn-DOX has tremendous potential for the development of "nanometronomic" chemotherapy toward safe and tailored oncological treatments.

Published
2017

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