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The rapid and accurate differentiation of Angelica sinensis and Levisticum officinale is a significant challenge in the quality control of traditional Chinese medicine. Angelica sinensis and Levisticum officinale are different genera of the same family. Due to their similar appearances and partially shared chemical components, unscrupulous traders often sell counterfeit Levisticum officinale as Angelica sinensis to make exorbitant profits. In this study, a fast and easy method of ultrasonic extraction and nebulization in real-time/carbon fiber ionization mass spectrometry (UENRT/CFI-MS) was introduced for the swift discrimination of these two easily confused traditional Chinese medicines. UENRT/CFI-MS primarily consists of an online extraction and nebulization unit, an ionization unit. The online extraction and nebulization unit employ an ultrasonic nebulizer chip for real-time sample extraction and nebulization. The ionization unit comprises a 10 mm long carbon fiber rod nested in a metal tube, achieving ionization through the application of a 3 kV high voltage, and the auxiliary solvent (methanol) is continuously supplied to the carbon fiber at a rate of 5 μL/min. Slices of Angelica sinensis and Levisticum officinale from different origins with similar size, appearance, and quality (diameter less than 16 mm, thickness not exceeding 0.5 cm) were selected and placed directly on the nebulizer chip. For each analysis, 200 μL methanol was continuously added using a pipette. When the nebulizer was activated, the lower end of the nebulizer chip generated a continuous spray of tiny droplets towards the carbon fiber tip for several seconds, which were then ionized before entering the mass spectrometer for detection. This device integrates online ultrasonic extraction, nebulization, and carbon fiber ionization technologies, enabling real-time analysis of complex samples without cumbersome pretreatment. By applying this technique, a characteristic ion peak of m/z 496 is uniquely identified in Levisticum officinale, serving as a distinctive marker for differentiation of Angelica sinensis and Levisticum officinale. Subsequently, comparative analysis was conducted using sample extracts on electrospray ionization mass spectrometry (ESI-MS) and carbon fiber ionization mass spectrometry (CFI-MS). These experimental results showed that CFI-MS exhibits superior selectivity and efficiency in detecting the m/z 496 characteristic peak, which is not observable in ESI-MS analysis. This finding highlights the unique ability of carbon fiber ionization to selectively detect certain compounds. The UENRT/CFI-MS technique was applied for the direct analysis of Angelica sinensis and Levisticum officinale, and can obtain the analysis results in just a few seconds. A distinctive ion of m/z 496 is detected in Levisticum officinale, facilitating swift discrimination between Angelica sinensis and Levisticum officinale. However, further research is needed to elucidate the structure of the m/z 496 characteristic peak. Additionally, the method’s applicability to a wider range of herbal medicines and its performance in more complex matrices should be investigated. This method is expected to play a role in traditional Chinese medicine identification.

Two new varieties from Xizang, China, i.e. Agapetes interdicta var. flaviflora and A. forrestii var. parvifolia, are described and illustrated. Agapetes interdicta var. flaviflora differs from the nominate variety in having yellow and smaller corollas with shorter lobes and anthers with shorter appendages at the base. Agapetes forrestii var. parvifolia is distinguished from the nominate variety in the smaller leaves with an acute apex, nearly transverse secondary veins and puberulent peduncle. Taxonomic notes on these taxa are also provided.

Abstract Background An elevated endogenous cortisol level due to the peripartum stress is one of the risk factors of postpartum bovine uterine infections. Selenium is a trace element that elicits anti-inflammation and antioxidation properties. This study aimed to reveal the modulatory effect of selenium on the inflammatory response of primary bovine endometrial stromal cells in the presence of high-level cortisol. The cells were subjected to lipopolysaccharide to establish cellular inflammation. The mRNA expression of toll-like receptor 4 (TLR4), proinflammatory factors, and selenoproteins was measured with qPCR. The activation of NF-κB and MAPK signalling pathways was detected with Western blot and immunofluorescence. Results The pretreatment with sodium selenite (2 and 4 µΜ) resulted in a down-regulation of TLR4 and genes encoding proinflammatory factors, including interleukin (IL)-1β, IL-6, IL-8, tumour necrosis factor α, cyclooxygenase 2, and inducible nitric oxide synthase. Selenium inhibited the activation of NF-κB and the phosphorylation of mitogen-activated protein kinase kinase, extracellular signal-regulated kinase, p38MAPK and c-Jun N-terminal kinase/stress-activated protein kinase. The suppression of those genes and pathways by selenium was more significant in the presence of high cortisol level (30 ng/mL). Meanwhile the gene expression of glutathione peroxidase 1 and 4 was promoted by selenium, and was even higher in the presence of cortisol and selenium. Conclusions The anti-inflammatory action of selenium is probably mediated through NF-κB and MAPK, and is augmented by cortisol in primary bovine endometrial stromal cells.

Abstract Background Sterol side-chain reductase 2 (SSR2) is a key enzyme in the synthesis of plant cholesterol pathway. Despite the importance of watermelon as a horticultural cash crop, the SSR2 gene in watermelon has not been previously studied or reported. Results In this study, 28 SSR2 genes were identified in the watermelon genome. The physicochemical properties of 28 ClaSSR proteins were predicted by bioinformatics methods, and the gene structure, conserved motif, chromosome localization, phylogenetic analysis, cis-acting elements, expression patterns, promoter activity analysis and subcellular localization of ClaSSRs were studied. The 28 ClaSSRs were unevenly distributed on 11 chromosomes, and phylogenetic analysis showed that they could be grouped into 4 groups with other related Cucurbitaceae homologous genes. Analysis of gene structure and motifs revealed similarities in exons/introns and motifs between members of the same group, further supporting phylogenetic results. The RT–qPCR results showed variations in ClaSSRs expression during watermelon fruit development. The analysis of promoter activity for ClaSSR25 showed strong activity. Subcellular localization studies confirmed that ClaSSR25 is mainly located in the cytoplasm, which aligns with the predicted outcomes. We additionally estimated the network of protein–protein interactions for ClaSSR25 and analyzed proteins that could potentially interact with ClaSSR25 in melon and Arabidopsis thaliana. Conclusions We conducted bioinformatics analysis and expression analysis of members of the watermelon SSR2 gene family in this work, and the outcomes set the stage for further investigations into the watermelon SSR2 gene. Graphical Abstract

Abstract Objective To establish a machine learning model based on radiomics and clinical features derived from non-contrast CT to predict futile recanalization (FR) in patients with anterior circulation acute ischemic stroke (AIS) undergoing endovascular treatment. Methods A retrospective analysis was conducted on 174 patients who underwent endovascular treatment for acute anterior circulation ischemic stroke between January 2020 and December 2023. FR was defined as successful recanalization but poor prognosis at 90 days (modified Rankin Scale, mRS 4–6). Radiomic features were extracted from non-contrast CT and selected using the least absolute shrinkage and selection operator (LASSO) regression method. Logistic regression (LR) model was used to build models based on radiomic and clinical features. A radiomics-clinical nomogram model was developed, and the predictive performance of the models was evaluated using area under the curve (AUC), accuracy, sensitivity, and specificity. Results A total of 174 patients were included. 2016 radiomic features were extracted from non-contrast CT, and 9 features were selected to build the radiomics model. Univariate and stepwise multivariate analyses identified admission NIHSS score, hemorrhagic transformation, NLR, and admission blood glucose as independent factors for building the clinical model. The AUC of the radiomics-clinical nomogram model in the training and testing cohorts were 0.860 (95%CI 0.801–0.919) and 0.775 (95%CI 0.605–0.945), respectively. Conclusion The radiomics-clinical nomogram model based on non-contrast CT demonstrated satisfactory performance in predicting futile recanalization in patients with anterior circulation acute ischemic stroke.

ABSTRACTStaphylococcus pseudintermedius (S. pseudintermedius) is a common pathogen that causes canine corneal ulcers. However, the pathogenesis remained unclear. In this study, it has been demonstrated that S. pseudintermedius invaded canine corneal epithelial cells (CCECs) intracellularly, mediating oxidative damage and pyroptosis by promoting the accumulation of intracellular reactive oxygen species (ROS) and activating the NLRP3 inflammasome. The canine corneal stroma was infected with S. pseudintermedius to establish the canine corneal ulcer model in vivo. The intracellular infectious model in CCECs was established in vitro to explore the mechanism of the ROS – NLRP3 signalling pathway during the S. pseudintermedius infection by adding NAC or MCC950. Results showed that the expression of NLRP3 and gasdermin D (GSDMD) proteins increased significantly in the infected corneas (p < 0.01). The intracellular infection of S. pseudintermedius was confirmed by transmission electron microscopy and immunofluorescent 3D imaging. Flow cytometry analysis revealed that ROS and pyroptosis rates increased in the experimental group in contrast to the control group (p

A reasonable ecological security pattern can improve landscape connectivity, promote biological circulation, maintain ecological processes, and improve regional ecosystem service functions and human well-being. Accordingly, systematically and scientifically analyzing the spatiotemporal evolution characteristics of the regional ecological security pattern has essential theoretical and practical significance. This research proposed that the analysis framework of the ecological security pattern should include a complete system composed of ecological sources, resistance surfaces, and corridors. Thus, this research uses the ecological network method to systematically analyze the spatiotemporal evolution characteristics of the ecological security pattern in the Qinba–Dabie intersection zone in China, a complete natural ecological unit across administrative regions, from 2000 to 2020. Results revealed the following: (1) The distribution change law of the ecological sources in the study area was consistent with the distribution change law of their centrality. (2) In the northeast of the study area, the disappearance of the small ecological sources, which play a stepping stone role, led to a longer length and a weaker centrality of ecological corridors. (3) The resistance surface in the study area showed an overall growth trend, while the ecological corridor network showed an overall deterioration trend. Based on the results, this research suggests that the systematic analysis and targeted protection of ecological sources are the basis for maintaining the ecological security pattern, and targeted improvement of ecological resistance surface is the key to improving the ecological security pattern. Therefore, measures such as the protection of core ecological sources, the rational layout of ecological sources as stepping stones, and the improvement of resistance values at ecological key points should be paid attention to in the future.

Objective: Patients with clear cell renal carcinoma (ccRCC) typically have poor prognosis. Glycosylation modification plays an important role in ccRCC. This study aimed to develop a novel signature for predicting ccRCC prognosis based on glycosylation-related genes (GRGs). Methods: Differentially expressed GRGs (DE_GRGs) were identified using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus and used for constructing the risk model. The function of key genes was validated. Results: Twenty-two DE_GRGs were intersected between GSE53757 and TCGA. Patients with ccRCC were divided into two clusters based on the expression profile of these DE_GRGs. Significant differences in the infiltration of 10 immune cell types were observed between two subclusters. Subsequently, the prognostic signatures of nine DE_GRGs (CHST9, COLGALT1, FUT3, FUT6, HS3ST2, POMGNT2, ST8SIA4, UGT3A1, and UGT8) were established. Patients in the high-risk group showed a poorer prognosis relative to the low-risk group. According to univariate and multivariate Cox regression analyses, the risk score, stage, and grade could be independent prognostic factors. A nomogram incorporating information on gender, age, risk group, TNM stage, and clinical stage showed accurate prediction in the survival probability. Except for CHST9, HS3ST2, and ST8SIA4, expression patterns of the remaining 6 DE_GRGs in Caki-1 and 786-O cells were confirmed by quantitative real-time PCR. FUT6 overexpression resulted in the inhibition of proliferation, migration, and invasion of ccRCC cells. Conclusion: This study established a nine-DE_GRG-based prognostic signature, which independently predicted ccRCC prognosis. This finding emphasizes that GRGs are stratification factors for the precise prognosis of ccRCC.

IntroductionCurrently, the large-scale consumption of fresh citrus fruits in the form of juices, jams, and purees results in significant quantities of waste consisting of citrus peels, pulp, and seeds.MethodsTo improve the utilization rate of whole citrus fruits and reduce the generation of processing waste, the best pre-optimized enzymatic fermentation conditions were used to treat whole citrus fruits and to analyse the changes in nutritional and active components (Enzymatic: pectinase, cellulase, hemicellulase added at a ratio of 1:1:1 for 63.326 min at a temperature of 60°C, with 0.506% of each enzyme added; Fermentation: Lacticaseibacillus rhamnosus TR08, Lactiplantibacillus plantarum subsp. Plantarum CICC 6257, and Limosilactobacillus fermentum CCFM1139 were added at a ratio of 1:1:1 for 30 h, with the concentration was 108 CFU/mL, and the total amount added was 6%).ResultsResults showed that after enzymatic hydrolysis, the mineral elements (Nitrogen, phosphorus), total amino acid, vitamin C, and organic acid content increased. For soluble sugars, the contents of fructose and glucose increased, whereas the sucrose content decreased. Fermentation had a minimal effect on the mineral content; however, the total amino acid, vitamin C, and soluble sugar contents decreased. Additionally, when comparing the samples after enzymatic fermentation to those after enzymatic hydrolysis alone, the total phenols, total flavonoids, carotenoids and antioxidant activities were significantly increased by 1.39, 1.49, 1.21 and 3.79 folds, respectively.DiscussionThis study addresses the challenges of utilizing fruit residue in the current market, including high treatment costs and environmental pollution, by providing a novel perspective on the enzymatic hydrolysis and fermentation of whole red oranges. Furthermore, it aims to improve the nutritional, functional, and health benefits of citrus fruit products.

This study aimed to investigate the effect of ultrasound treatment times (30 min and 60 min) and levels of quinoa protein (QPE) addition (1 % and 2 %) on the quality of Chinese style reduced-salt pork meatballs, commonly known as lion’s head. The water-holding capacity (WHC), gel and rheology characteristics, and protein conformation were assessed. The results indicated that extending the ultrasound treatment time and elevating the quinoa protein content caused conspicuous improvements (P

Due to the complexity and diversity of the chemical composition of traditional Chinese medicines (TCMs), the identification of components of TCMs can effectively guarantee the quality of TCMs and be of great significance for the development and utilization of TCMs. In this study, the ultra-high performance liquid chromatography-quadrupole-electrostatic field Orbitrap mass spectrometry (UHPLC-Q-Orbitrap/MS) was used to comprehensively analyze the chemical composition of Danggui Sini Granules. The UHPLC separation was performed on a SUPELCO C18 column (100 mm×4.6 mm×2.7 μm), and the column temperature was 30 ℃, the injection volume was 5 μL. The mobile phase was consisted of 0.1% formic acid aqueous solution (A) and acetonitrile (B) with the flow rate of 0.5 mL/min. MS data was collected under positive and negative ion modes. A self-constructed database of organic acids, flavonoids, phthalides, triterpenoids, saponins and other compounds was established by searching online databases. Rapid identification of the constituents of Danggui Sini Granules based on precise m/z and secondary fragment ion information in combination with literature data was achieved, and the herbal sources of the compounds were attributed. Seventy-two compounds are identified from Danggui Sini Granules, including 20 flavonoids, 15 organic acids, 11 saponins, 9 terpenoids, 5 phthalides, 1 phenylpropanoid, 1 aldehyde, 1 nucleoside, 1 lignan and 8 other compounds. Among them, the organic acids and phthalides are mainly from Angelicae Sinensis Radix, the flavonoids are mainly from Glycyrrhizae Radix et Rhizoma, the saponins are mainly from Akebiae Caulis, the terpenoids are mainly from Paeoniae Radix Rubra, the nucleosides are mainly from Jujubae Fructus, and other compounds are mainly from Asari Radix et Rhizoma and Cinnamomi Cortex. The results showed that the chemical composition of this compound preparation is rich and structurally diverse, which not only provides data support for comprehensive understanding of its chemical composition, but also a preliminary research basis for clarifying the material basis of its pharmacological action. However, there are still some unknown species whose specific structures have not been clarified. The structural identification and differentiation of these compounds are somewhat limited in the absence of sufficient controls, and can be followed up with deeper separation and identification by techniques such as nuclear magnetic resonance mass spectrometry (NMR-MS).

The ginsenoside isomers were analyzed and distinguished by using inlet ionization-high resolution mass spectrometry technology under positive and negative ion modes with optimum inlet temperatures. The results showed that under the full scanning mode of positive and negative ion modes with the optimum experimental temperature, different types of ions were formed according to the characteristics of different numbers of polymers formed by isomeric monomers of ginsenoside, and the identification of ginsenoside isomers was realized. For the identification of ginsenoside CK and Rh2, there was no obvious difference in their MS spectra under positive ion mode. However, under negative ion mode with the inlet temperature of 200 ℃, ginsenoside CK tended to form polymers of [M+Cl]－, Rh2 tended to form polymers of [M+COOH]－, and the number of polymers of CK was significantly higher than that of Rh2. For the identification of ginsenoside Rb2 and Rc, under positive ion mode with the inlet temperature of 200 ℃, ginsenoside Rb2 and Rc could produce polymers of [M+Na]+, but the polymers peak intensity of Rb2 was significantly higher than that of Rc. Under negative ion mode with the inlet temperature of 200 ℃, Rb2 tended to form polymers of [M+Cl]－, and Rc tended to form polymers of [M+COOH]－. For the identification of ginsenoside Rg1 and Rf, there was no obvious difference in their MS spectra under the positive ion mode. Under negative ion mode with the inlet temperature of 200 ℃, Rg1 and Rf tended to form polymers of [M+Cl]－ and [M−H]−, respectively. In this study, the three pairs of isomers in ginsenosides were basically identified by using the full MS scanning method and the control of the mass spectrometry inlet temperature. The experimental method is simple, fast, and has the characteristics of low consumption. This study confirms that the entrance ionization mass spectrometry technique can provide a theoretical reference for the rapid discrimination and identification of ginsenoside isomers.

Abstract Grassland caterpillars (Lepidoptera: Lymantriinae: Gynaephora) are the most damaging pests to alpine meadows in the Qinghai–Tibetan Plateau (QTP). Here, we conducted extensive sampling from 39 geographic populations covering almost the entire distribution of the eight QTP Gynaephora (Hübner) species to investigate phylogeographic patterns and speciation based on two mitochondrial genes (COI and ND5). A total of 40 haplotypes were detected in the 39 populations, with >70% of all haplotypes not shared between populations. The monophyletic QTP Gynaephora migrated from non‐QTP regions during the Pliocene, corresponding to the uplift of the QTP, suggesting a mode of transport into the QTP. Among the eight QTP Gynaephora species described by morphological characteristics, two species (G. alpherakii and G. menyuanensis) were recovered as monophyletic groups (Clades B and C), while the remaining six formed two monophyletic clades: Clade A (G. qinghaiensis, G. jiuzhiensis, and G. qumalaiensis) and Clade D (G. aureata, G. ruoergensis, and G. minora). These results suggested that the number of the QTP Gynaephora species may be overestimated and further studies based on both morphological and nuclear gene data are needed. Genetic differentiation and speciation of the QTP Gynaephora were likely driven by the QTP uplifts and associated climate fluctuations during the Pleistocene, indicated by divergence time estimation, suggesting that isolation and subsequent divergence was the dominant mode of speciation. The Sanjiangyuan region (i.e., Clade A, characterized by high genetic diversity) may have been a glacial refugium of the QTP Gynaephora, as supported by analyses of gene flow and biogeography. High levels of genetic diversity were found in QTP Gynaephora, without population expansion, which may explain the high‐altitude adaptation and outbreaks of grassland caterpillars in alpine meadows of the QTP. This study provides the largest phylogeographic analysis of QTP Gynaephora and improves our understanding of the diversity and speciation of QTP insects.

AimComparing the safety, effectiveness, and mid-term survival rates of robot-assisted minimally invasive esophagectomy (RAMIE) and video-assisted minimally invasive esophagectomy (VAMIE).MethodsA total of 842 patients undergoing minimally invasive esophagectomy were analyzed, including 694 patients in VAMIE group and 148 in RAMIE group. PSM analysis was applied to generate matched pairs for further comparison. Operative outcomes, postoperative complications and Mid-term outcomes were compared between all patients in matched groups.ResultsAfter 1:4 PSM, 148 patients in the RAMIE and 592 patients in the VAMIE. Compared to VAMIE, RAMIE exhibited earlier removal of chest and neck drainage tubes, shorter postoperative hospital stays, and a higher number of lymph node dissections. However, the surgical duration of RAMIE was longer than that of VAMIE. Postoperative complications were no statistically significant between the RAMIE and VAMIE groups. There was no statistically significant difference in the 3-year OS and DFS between the two groups.ConclusionCompared to VAMIE, RAMIE emerges as a viable and safe surgical approach and suggests RAMIE as a potential alternative to minimally invasive esophagectomy.

Angelica dahurica root (ADR), a commonly utilized herbal medicine in China and other Asian nations, which has anti-inflammatory effects on diverse inflammatory ailments. However, the bioactive components and underlying mechanism responsible for the anti-inflammatory effect of ADR are still unclear. This work attempted to discover the anti-inflammatory bioactive compounds and explore their underlying mechanism in ADR based on spectrum-effect relationship analysis and NF-κB signaling pathway. Chromatographic fingerprints of ADR samples were established by high performance liquid chromatography with diode array detection (HPLC-DAD), and a total of eleven common peaks were selected. Then, high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS) was employed for identification of eleven common peaks in ADR Meanwhile, the anti-inflammatory activities of ADR samples were assessed by inhibition of NO, interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) production in LPS-induced RAW264.7 cells. The spectrum-effect relationships between the eleven common peaks in HPLC fingerprints and anti-inflammatory effects of ADR samples were investigated to identify the potential anti-inflammatory bioactive compounds by grey relational analysis (GRA) and partial least squares regression (PLSR). The spectrum-effect relationship analysis results indicated that six coumarin compounds, including bergapten, xanthotoxin, phellopterin, isoimperatorin, xanthotoxol and imperatorin could be potential anti-inflammatory bioactive compounds in ADR. The further validation experiments also showed that these six coumarins demonstrated significant inhibition of NO, IL-1β, IL-6, and TNF-α production in LPS-induced RAW264.7 cells. In addition, western blot analysis was conducted to explore the mechanisms of two potential anti-inflammatory bioactive compounds (phellopterin and isoimperatorin) by assessing the protein levels in the NF-κB signaling pathway. The western blot results illustrated that phellopterin and isoimperatorin could significantly down-regulate the phosphorylated NF-κB p65 (p-p65), phosphorylated IκBα (p-IκBα) and iNOS, and depress the pro-portion of p-p65/p65 and p-IκBα/IκBα, which indicated that these two coumarins in ADR could potentially exert anti-inflammatory effects by suppressing of NF-κB pathway.

Abstract Background Endometritis is a common bovine postpartum disease. Rapid endometrial repair is beneficial for forming natural defense barriers and lets cows enter the next breeding cycle as soon as possible. Selenium (Se) is an essential trace element closely related to growth and development in animals. This study aims to observe the effect of Se on the proliferation of bovine endometrial epithelial cells (BEECs) induced by lipopolysaccharide (LPS) and to elucidate the possible underlying mechanism. Results In this study, we developed a BEECs damage model using LPS. Flow cytometry, cell scratch test and EdU proliferation assay were used to evaluate the cell cycle, migration and proliferation. The mRNA transcriptions of growth factors were detected by quantitative reverse transcription-polymerase chain reaction. The activation of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) and Wnt/β-catenin pathways were detected by Western blotting and immunofluorescence. The results showed that the cell viability and BCL-2/BAX protein ratio were significantly decreased, and the cell apoptosis rate was significantly increased in the LPS group. Compared with the LPS group, Se promoted cell cycle progression, increased cell migration and proliferation, and significantly increased the gene expressions of TGFB1, TGFB3 and VEGFA. Se decreased the BCL-2/BAX protein ratio, promoted β-catenin translocation from the cytoplasm to the nucleus and activated the Wnt/β-catenin and PI3K/AKT signaling pathways inhibited by LPS. Conclusions In conclusion, Se can attenuate LPS-induced damage to BEECs and promote cell proliferation and migration in vitro by enhancing growth factors gene expression and activating the PI3K/AKT and Wnt/β-catenin signaling pathways.

Plectasin, a novel antimicrobial peptide, has the potential to disrupt bacterial cell walls and alter the rumen fermentation mode, making it a superior alternative to antibiotics. However, there is limited research on the effects of plectasin on rumen microbiota. This study aimed to evaluate the effects of plectasin (0.057 μmol/L) on in vitro rumen fermentation characteristics and select groups of rumen bacterial communities in comparison with monensin (5 μmol/L), one of the most commonly used ionophores in ruminants, and as a control treatment with the basal substrate. Unlike monensin, plectasin was found to increase the molar proportions of butyrate and acetate/propionate ratio (p < 0.001) while decreasing pH and the molar proportions of propionate (p < 0.05). Principal component analysis of bacterial 16S rRNA gene amplicons clearly showed a separation between the bacteria shaped by plectasin and monensin. Comparative analysis also revealed differences in the relative abundance of certain bacteria in different taxa between plectasin and monensin. The divergent effects of plectasin and monensin on bacterial communities are likely responsible for the differences in their ability to alter rumen fermentation. Plectasin may have advantages over monensin in modulating ruminal bacterial communities and increasing the butyrate and the acetate/propionate ratio. Therefore, it may be considered as a potential additive for ruminant feed.

High-resolution topographic and geomorphic data are important basic data for the study of active structures. Here, multisource remote sensing data were used to reinterpret the active faults in the northern segment of the Red River Fault (China). First, we obtained airborne light detection and ranging (LiDAR) data, high-resolution GaoFen-7 (GF-7) remote sensing image data, and historical aerial photographs, and a high-resolution digital elevation model (DEM) was generated based on the airborne LiDAR data and GF-7 data. According to the remote sensing interpretation, the main active faults were identified. We subsequently verified the faults in the field and constrained the geographic locations. The current activity was confirmed to be dominantly normal faulting, with some dextral strike-slip components, and the latest active age was the Late Holocene. It reflects the coordination of structural deformation between the rotation of the secondary block and the sliding of the boundary fault within the Sichuan–Yunnan Block. The results show that airborne LiDAR and GF-7 remote sensing data have a great application value in providing high-resolution topographic and geomorphologic data for the study of active structures. The comprehensive application of multisource remote sensing data can greatly improve the reliability of active fault interpretations and provide a reference for follow-up research within the study area.

Endometritis represents a prevalent condition in perinatal dairy cows. Bovine endometrial epithelial cells (BEECs), as the primary interface between cavity and the external environment, are particularly vulnerable to infection by pathogenic bacteria following parturition. A20 is essential for regulating inflammation and modulating immune responses. Nevertheless, the exact role of A20 in the BEECs in response to inflammatory response is not fully understood. An endometritis model infected by Escherichia coli (E. coli) in vivo and a BEECs inflammation model induced with lipopolysaccharide (LPS) in vitro were built to investigate the function and governing mechanisms of A20 in endometritis. The results showed that infection with E. coli resulted in endometrial damage, inflammatory cell infiltration, and upregulation of inflammatory factors in dairy cows. Furthermore, A20 expression was upregulated in the endometrium of cows with endometritis and in BEECs following LPS stimulation. A20 overexpression attenuated the level of proinflammatory cytokines in LPS-stimulated BEECs; conversely, A20 knockdown lead to an exacerbated response to LPS stimulation. The overexpression of A20 was shown to activate autophagy and suppress the NF-κB signaling pathway in LPS-stimulated BEECs. However, blocking autophagy with chloroquine notably attenuated the anti-inflammatory effect of A20, leading to the activation of the NF-κB signaling pathway. In summary, the study demonstrated that A20’s suppression of inflammation in LPS-stimulated BEECs is associated with the activation of autophagy. Therefore, the A20 protein showed potential as a novel treatment focus for managing endometritis in dairy cows.

Previous studies have shown that polymethoxylated flavonoids-loaded citral emulsion (PCT) can inhibit the growth and reproduction of Penicillium in citrus; however, PCT is difficult to apply to fruit preservation due to its high fluidity and volatility. Therefore, in this study, we combined PCT with chitosan (CS) to investigate the effect of a composite coating on citrus preservation. The results showed that compared to the control group, the CS-PCT group could effectively reduce the decay rate and maintain moisture availability, color difference, and hardness. Moreover, the contents of nonenzymatic antioxidants and volatile substances with antimicrobial activity were better preserved. In addition, the activities of related antioxidant enzymes were greater in the treatment group, and the expression of the corresponding enzyme-encoding genes was upregulated. Consequently, CS-PCT treatment could effectively maintain fruit quality and improve the resistance of citrus fruits during storage; moreover, it can be considered a nontoxic and efficient citrus preservative.

ObjectiveEphedra, widely used in clinical practice as a medicinal herb, belongs to the genus Ephedra in the family Ephedraceae. However, the presence of numerous Ephedra varieties and variants requires differentiation for accurate identification.MethodsIn this study, we employed headspace gas chromatography mass spectrometry (HS-GC-MS), ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and global natural products social molecular networking (GNPS) for chemical component identification. Chemometric analysis was used to analyze the differential components. Metabolic analysis and Kyoto encyclopedia of genes and genomes (KEGG) enrichment were utilized to explore the synthesis pathways of different components.ResultA total of 83 volatile and 79 non-volatile components were identified in Ephedra species. Differential analysis revealed that among the eight Ephedra stems, 18 volatile and 19 non-volatile differential compounds were discovered, whereas Ephedra roots exhibited 21 volatile and 17 non-volatile markers. Volatile compounds were enriched in four synthetic pathways, while non-volatile components were enriched in five pathways among the differentiated components.ConclusionThis study is the first to conduct a comparative analysis of chemical components in different Ephedra species and parts. It provides a foundational reference for authenticating Ephedra herbs, evaluating medicinal resources, and comparing quality in future studies.

Unilateral Tracheobronchial foreign body (TFB) present a common clinical disease, whereas bilateral TFB is a rare and acute condition associated with high mortality rates. This case study discusses a pediatric patient hospitalized due to respiratory distress following accidental ingestion of peanut kernels. A plain chest CT scan revealed obstructive emphysema in the right main bronchus and a foreign body at the opening of the left main bronchus. Surgical removal of the bilateral TFB under extracorporeal circulation resulted in a successful postoperative recovery, leading to discharge on the 9th day. A comprehensive literature search was conducted across databases including PubMed, Web of Science, EMBASE, Cochrane Library, and CNKI, spanning publications from January 2014 to October 2023, utilizing keywords “bronchial foreign body” and “Peanut”. After deduplication and relevance screening, 9 pertinent literature sources were included. The objective of this study is to enhance clinical practitioners' understanding of TFB management and improve diagnostic and treatment capabilities through analysis of age of onset, clinical manifestations, diagnosis, and treatment approaches in critically ill pediatric patients.

Forsythia suspensa (Thunb.) Vahl (FS) is an important plant with high edible and medicinal values. The edible fruit of FS is a common traditional medicine in China, Japan and Korea. Compared to the research on the phytochemistry and pharmacology of the fruits, study on the other parts of FS, such as leaves and flowers is still limited. In this study, an integrated strategy based on ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS), plant metabolomics and correlation analysis was established for comprehensively chemical characterization of fruits, leaves and flowers of FS, and discovery of α-glucosidase and pancreatic lipase inhibitory metabolites. The plant metabolic profiling of fruits, leaves and flowers of FS was performed by UPLC-QTOF-MS/MS, and a total of 74 secondary metabolites, including 15 phenylethanoid glycosides, 20 lignans, 10 cyclohexanol derivatives, 11 organic acids, 9 flavonoids, 3 triterpenes, and 10 other compounds were identified. Then, 29 differential secondary metabolites that responsible to distinguish the fruits, leaves and flowers of FS were further screened out by multivariate statistical analysis. Meanwhile, the α-glucosidase and pancreatic lipase inhibition of different parts of FS were evaluated and compared by in vitro experiments. The results demonstrated that the leaves of FS showed the highest inhibition on α-glucosidase and pancreatic lipase with IC50 of 0.17 ± 0.04 mg/mL and 0.56 ± 0.33 mg/mL, respectively. Then, the correlation between differential metabolites and enzyme inhibitory activities were investigated by Pearson correlation analysis, and 12 potential α-glucosidase and pancreatic lipase inhibitors were screen out. Additionally, the α-glucosidase and pancreatic lipase inhibitory activities of five potential enzyme inhibitory components, including quercitrin, rutin, kaempferol-3-O-rutinoside, pinoresinol-4-O-glucoside and phillyrin were further validated by in vitro assays and molecular docking analysis. The results showed that all the five potential inhibitors showed good inhibitory effects on α-glucosidase and pancreatic lipase, and the binding of the five inhibitors to enzymes mainly through hydrogen bonding, hydrophobic force, and ionic bonding. This study provided a feasible strategy for comparison and discrimination of different parts of medicinal plant and discovery of bioactive components, and also provided useful information for future utilization of different parts of FS.

The resonant excitation of Bloch Surface Waves (BSWs) in dielectric one-dimensional photonic crystals is becoming a realistic photonic solution for surface integration in many domains, from spectroscopy to local field management. Bringing BSWs to ultrafast and nonlinear regimes requires a deep knowledge of the effects that the photonic crystal dispersion and the resonant surface wave excitation have on the ultrashort laser pulses. We report on the experimental evidence of spectral and temporal modifications of the radiation leaving a planar one-dimensional photonic crystal after coupling to BSWs. In such a resonant condition, a characteristic long temporal tail is observed in the outgoing pulses. Observations are performed by employing both frequency-resolved optical gating and field cross-correlation techniques.

Scientific quantitative measurement of the coupling relationship between urban population (UP) and urban land (UL) is conducive to promoting intensive land use, coordinated human–land development, and new urbanization development. This research proposed an improved coupling model of polar coordinates to study the spatiotemporal coupling characteristics between UP and UL at the prefecture-level city scale in China from 2010 to 2020. The study results include the following: (1) The growth rate of UL in China’s prefecture-level cities was higher than that of UP. The per capita urban land (PUL) was always within the ideal range, and the dynamic human–land coupling state remained reasonable. (2) The UP, UL, and dynamic human–land coupling rationality in China showed characteristics that were high in the east and low in the west. The PUL showed characteristics that were high in the northwest and low in the southeast. (3) This study divided China’s prefecture-level cities into four regions according to the coupling characteristics and formulated regionally differentiated optimization strategies. In summary, this study carried out a more scientific and reasonable quantitative measurement of the coupling relationship between UP and UL in China, resulting in a more reliable and targeted formulation of optimization strategies.

Boric acid (BA) is commercially acceptable and economically feasible material to enhance the shelf life of pears, oranges and other horticultural plants. Here, we investigated the effect of BA on the shelf life and post-harvest quality of table grapes (cv. 'Kyoho'). The grapes were immersed in a BA solution with different concentrations [0 (as the control), 10, 30, 50 mM] for 10 min and stored at 25 ± 1 °C for 10 days. Compared to the control, the BA treatments maintained higher berry firmness by inhibiting the activity of polygalacturonase (PG) and cellulase. In addition, the BA-treated grapes maintained higher antioxidant enzyme activities, such as catalase (CAT) and superoxide dismutase (SOD), and lower metabolic toxic products, like the superoxide anion (O2-) production rate, malondialdehyde (MDA) and hydrogen peroxide (H2O2) content than the control. The experimental results showed that the post-harvest application of BA effectively delays the senescence of grapes compared with the control, and the 10 mM BA treatment had the most obvious effect.

In recent years, the types and quantities of fentanyl analogs have increased rapidly. It has become a hotspot in the illicit drug control field of how to quickly identify novel fentanyl analogs and to shorten the blank regulatory period. At present, the identification methods of fentanyl analogs that have been developed mostly rely on reference materials to target fentanyl analogs or their metabolites with known chemical structures, but these methods face challenges when analyzing new compounds with unknown structures. In recent years, emerging machine learning technology can quickly and automatically extract valuable features from massive data, which provides inspiration for the non-targeted screening of fentanyl analogs. For example, the wide application of instruments like Raman spectroscopy, nuclear magnetic resonance spectroscopy, high resolution mass spectrometry, and other instruments can maximize the mining of the characteristic data related to fentanyl analogs in samples. Combining this data with an appropriate machine learning model, researchers may create a variety of high-performance non-targeted fentanyl identification methods. This paper reviews the recent research on the application of machine learning assisted non-targeted screening strategy for the identification of fentanyl analogs, and looks forward to the future development trend in this field.

Angelica dahurica is a medicinal herb of the Umbelliferae family. The dried root of A. dahurica, also known as Angelicae dahuricae Radix, is widely used in clinical treatment. However, the aboveground part of A. dahurica which accounted for over 70% of the total plant was abandoned in the field. In order to develop the value of the aboveground part of A. dahurica, the chemical constituents and arginine kinase (AK) inhibitory activity of A. dahurica leaves were studied. 85 volatile components were identified from A. dahurica leaves by GC-MS; 39 non-volatile components including sugars, amino acids and organic acids were identified by pre-column derivatization GC-MS analysis; and 7 coumarins were qualitatively and quantitatively analyzed by HPLC. Then, an inhibitory enzyme-linked immunosorbent assay (iEIA) was applied for evaluation of AK inhibitory activity. The extracts of A. dahurica leaves exhibited well inhibitory effects on AK. Further, potential AK inhibitors were screened by grey relational analysis and their inhibitory activities were validated by iEIA. l-aspartic acid exhibited strongest inhibitory effect on AK with its IC50 value was 0.558 mM, which was much lower than that of chlorpheniramine (6.644 mM). The obtained chemical profiles displayed chemical diversity of A. dahurica leaves and will provide data support for the future development and utilization of A. dahurica leaves. The screened potential AK inhibitors from A. dahurica leaves could be candidates for development of antiallergic substances or insecticides.

Background: Along with existing infection control policies, repeated education and training of environmental service workers (ESWs) improves their compliance and ultimately reduces hospital-associated infection (HAI) rates. However, only limited studies have explored the health behavioral determinants of ESWs regarding their cleaning performance after implementing an educational intervention with multi-faceted infection control strategy. Objective: To determine whether an educational intervention with multi-faceted infection control strategy improves the health behavioral determinants associated with ESWs’ cleaning performance. Methods: Twenty-eight ESWs who received an educational intervention with multi-faceted hospital infection control strategy were included. ESWs’ knowledge, perceived benefits and barriers, self-efficacy, health literacy, and cleaning performance were evaluated at pre-intervention, post-intervention, and 3-month follow-up. Results: HAI-related adenosine triphosphate (ATP) levels decreased significantly at post-intervention and 3-month follow-up compared with pre-intervention levels (all p

Magnetic reconnection and current disruption are two key processes in driving energy conversion and dissipation in planetary magnetospheres. At the Earth, the two processes usually occur at different locations: the current disruption process occurs more frequently in the near-Earth magnetotail ∼10 R _E , while the magnetotail reconnection process is expected to take place in the more distant region where the current sheet is thinner. Occasionally, under very intense solar wind perturbations, reconnection could be located closer to the Earth where current disruption processes usually operate. But it is unclear what the situation is at giant planets, in which the plasma environment is very different from the Earth. In this study, we investigate a middle-Jupiter reconnection event at ∼43 R _J . During the event, the inferred integrated cross-field currents were substantially reduced, which we argue is a signature of current disruption. In this case, we suggest that magnetic reconnection could be colocated with a current disruption process in the Jovian magnetosphere, which is roughly analogous to the situation in the extremely perturbed Earth’s magnetosphere.

Long non-coding RNAs (lncRNAs) are more than 200 bp in length and do not translate into functional proteins. They are involved in inhibiting cell growth, migration, and invasion, and in promoting apoptosis. It was found that cancer-related LINC00961 is downregulated in a variety of malignant tumors, including lung cancer, renal cell carcinoma, and hepatocellular carcinoma. LINC00961 either inhibits or promotes the expression of many genes to affect cancer progression via microRNAs such as miR-5581-3p, miR-367, miR-223-3p, miR-19-3p, and miR-125b-5p, epithelial–mesenchymal transition, and Wnt/β-catenin signaling pathway. Thus, LINC00961 may be a viable biomarker or therapeutic target for human cancers. In this review, we summarize the current evidence of the biological functions of LINC00961 in tumor development, to provide new insights and ideas for molecular targeted therapy for patients with cancer. Abbreviations: CRLS1, cardiolipin synthase 1; EMT, epithelial–mesenchymal transformation; HCC, hepatocellular carcinoma; LncRNA, long non-coding RNA; LUAD, lung adenocarcinoma; NSCLC, non-small cell lung cancer; OSCC, oral squamous cell carcinoma; PCAN, proliferating cell nuclear antigen; PTEN, phosphatase and tensin homologue; RCC, renal cell carcinoma; SPAR, small regulatory polypeptide of amino acid response; TSCC, tongue squamous cell carcinoma; VSMCs, vascular smooth muscle cell.

Abstract Background Cadmium (Cd) is a highly toxic element for plant growth. In plants, hydrogen sulfide (H2S) and methylglyoxal (MG) have emerged as vital signaling molecules that regulate plant growth processes under Cd stress. However, the effects of sodium hydrosulfide (NaHS, a donor of H2S) and MG on Cd uptake, physiological responses, and gene expression patterns of Salix to Cd toxicity have been poorly understood. Here, Salix matsudana Koidz. seedlings were planted in plastic pot with applications of MG (108 mg kg− 1) and NaHS (50 mg kg− 1) under Cd (150 mg kg− 1) stress. Results Cd treatment significantly increased the reactive oxygen species (ROS) levels and malondialdehyde (MDA) content, but decreased the growth parameters in S. matsudana. However, NaHS and MG supplementation significantly decreased Cd concentration, ROS levels, and MDA content, and finally enhanced the growth parameters. Cd stress accelerated the activities of antioxidative enzymes and the relative expression levels of stress-related genes, which were further improved by NaHS and MG supplementation. However, the activities of monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) were sharply decreased under Cd stress. Conversely, NaHS and MG applications restored the MDHAR and DHAR activities compared with Cd-treated seedlings. Furthermore, Cd stress decreased the ratios of GSH/GSSG and AsA/DHA but considerably increased the H2S and MG levels and glyoxalase I-II system in S. matsudana, while the applications of MG and NaHS restored the redox status of AsA and GSH and further improved glyoxalase II activity. In addition, compared with AsA, GSH showed a more sensitive response to exogenous applications of MG and NaHS and plays more important role in the detoxification of Cd. Conclusions The present study illustrated the crucial roles of H2S and MG in reducing ROS-mediated oxidative damage to S. matsudana and revealed the vital role of GSH metabolism in regulating Cd-induced stress.

Abstract Background Many women experience menstruation-related health issues during their child-bearing years. This study aimed to evaluate women’s tendency to seek Traditional Chinese Medicine (TCM) and/or Western Medicine (WM) when newly diagnosed with menstrual syndromes and to identify factors associated with their medical care-seeking behaviors. Methods The data of a total of 47,097 women aged between 15 and 50 years with newly diagnosed menstrual syndromes in 2005 were extracted from the Taiwan National Health Insurance Database. The follow-up period was divided equally into 6 month segments over 5 years starting with patients’ first visit for obstetric/gynecologic care. Outcomes were outpatient visits and number of TCM or WM visits during each period. Patients’ tendency for medical care utilization was estimated using Poisson regression analysis. Results Number of outpatient visits using TCM was 0.62 (29187/47097), and using WM was 1.67 (78697/47097) within 6 months after the first menstrual syndrome diagnosis. The tendency for TCM utilization increased as follow-up time increased after controlling for potential confounders, while WM utilization decreased as follow-up time increased. Age, economic status, infertility, value of prevention, baby delivery, and obstetric/gynecologic inpatient histories were significantly associated with patients’ medical care-seeking behaviors. Conclusions TCM and WM medical care-seeking patterns are significantly different among women with diagnoses associated with menstrual syndromes. Related factors affecting medical care-seeking behavior include age, economic status, infertility, value of prevention, childbirth and Ob/Gyn inpatient histories.