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Several trials with young pigs were conducted during an experimental period of 6 weeks to evaluate the effects on growth and immune responses of dietary supplementation with 10, 25 and 50 g/kg of E-CAB-94011, a fish extract rich in proteins, unsaturated fatty acids and endogenous anti-oxidants. This extract was obtained using a lyophilization process consisting of three phases: freezing, primary drying and secondary drying. Conditions in the dryer were varied through the cycle to ensure that the resulting product had the desired physical and chemical properties, and that the required stability was achieved. A total of 360 pigs were randomly allocated according to initial bodyweight and divided into four groups of 90 pigs. The control diet contained hard red spring wheat, spray-dried whey and soybean meal. The treatment diets were the control plus 10 g/kg, 25 g/kg and 50 g/kg of E-CAB-94011. Growth performance, plasma biochemical values, hematological values and humoral and cellular immune responses were measured. Results indicated that supplementation with E-CAB-94011 affects both growth and immune response. When the growth parameters of pigs were compared, the largest percentage improvement in growth was observed from weaning to 8–10 weeks of age. These experiments confirm that the use of E-CAB-94011 results in significant improvements, even when the herd of origin has a relatively high health status. [ABSTRACT FROM AUTHOR]

Many clinical conditions exist in which it is desirable to stimulate or suppress the immune system, and many different drugs are able to do this. It is also well known that nutrition may affect human health and immune responses. Nutritional factors are crucial components of the diet and essential for normal growth and development of both vertebrate and invertebrate organisms. Many of these components have been shown to play different roles in the immune response and, under different circumstances, they can significantly modulate the immune system to create an effective response. Diet and its components are known to play an important factor in the process of inflammation and in turn on the health effects related to inflammation, such as cancer and cardiovascular diseases. Previous research so far has mainly looked at the effect of specific food stuffs or nutrients on inflammation and health outcomes. The aims of the present review was a) to underline the fact that diet as a whole plays an important role in modifying inflammation and health outcomes related to inflammation, aging, and colon cancer; b) to show the in vitro cytotoxic effect of LipoFishins (E-Congerine 10423 ® ; AntiGan™) obtained from the Atlantic Conger conger marine organism present on the Galician coast, against different human tumor cell lines; c) to show the in vivo effect of E-Congerine-10423 ® on colonic inflammation induced in mice by seven weeks' exposure to 2% of dextran sulfate sodium (DSS); and d) to show the effect of E-Congerine-10423 ® (AntiGan™) on tumor markers (TMs) in healthy subjects and in patients with different types of cancer at the time of diagnosis. Preliminary data in a limited number of cases indicate that about 50% of the patients show a reduction in the levels of tumor markers (TM), and this response was much more evident in patients with cancer, when TM values are above normal range. Finally, all the above mentioned results suggest that diet has a major role in controlling inflammation and thereby plays an important role in the development or prevention of various chronic diseases, hence public health steps should be taken to modify the individual's whole diet and to promote the intake of specific natural compounds., (Copyright © 2017. Published by Elsevier Ltd.)

Novel effective chemopreventive agents against cancer are required to improve current therapeutic rates. The aim of the present study was to investigate the anti-carcinogenesis effect of AntiGan, an extract obtained from the European conger eel, Conger conger , in vitro (human tumor cell lines) and in vivo (murine model of colitis) models. The potential apoptogenic activity after 24 h of incubation with 10, 25 and 50 µl/ml AntiGan was reported using growth inhibition and apoptosis activity assays. In vivo studies were performed in mice by inducing colitis with oral administration of 2% dextran sulphate sodium (DSS) for 5 weeks. Apoptosis was observed in HL-60, Hs 313.T, SW-480, Caco-2 and HT-29 cell lines. The highest level of growth inhibition was observed in Caco-2 (66, 75.8 and 88.1%), HT-29 (56, 73 and 87.6%) and SW-480 (38.5, 61.6, 78.6%) for AntiGan doses of 10, 25 and 50 µl/ml, respectively, compared to untreated cells, while the results of the expression of genes associated with apoptosis indicated a downregulation of B-cell lymphoma 2 (Bcl-2) in all cell lines studied. In vivo , morphopathological alterations in the colon were analyzed by immunohistochemical and staining methods. Tumoral markers, including β-catenin, cyclooxygenase 2 and Bcl-2 were expressed in cryptal cells of the dysplastic colonic mucosa, whereas the levels of interferon-γ expression were also increased when no treatment was applied. In the experimental murine model, the optimal concentration of AntiGan for an effective dose-response was 10% in diet. These results suggested that AntiGan displays a powerful anti-inflammatory effect in DSS-induced colitis, acting as a chemopreventive agent against colon carcinogenesis, most likely due to its apoptogenic peptides that contribute to the induction of apoptosis.