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17 results on '"Lokesh, Veeresh"'

3. Nano-structured hydrotrope-caged cytochrome c with boosted stability in harsh environments: a molecular insight.

Author

Bharadwaj, Pranav, Sarkar, Dheeraj Kumar, Bisht, Meena, Shet, Sachin M., Kotrappanavar, Nataraj Sanna, Lokesh, Veeresh, Franklin, Gregory, Brezovsky, Jan, and Mondal, Dibyendu

Subjects
ENZYMES, PROTEOLYSIS, REACTIVE oxygen species, BIOCATALYSIS, BINDING sites, CYTOCHROME c
Abstract

Green and nano-structured catalytic media are vital for biocatalysis to attenuate the denaturation tendency of biocatalysts under severe reaction conditions. Hydrotropes with multi-faceted physiochemical properties represent promising systems for sustainable protein packaging. Herein, the ability of adenosine-5′-triphosphate (ATP) and cholinium salicylate ([Cho][Sal]) ionic liquid (IL) to form nano-structures and to nano-confine Cytochrome c (Cyt c) enhanced the stability and activity under multiple stresses. Experimental and computational analyses were undertaken to explain the nano-structured phenomenon of ATP and IL, structural organizations of nano-confined Cyt c, and site-specific interactions that stabilize the protein structure. Both ATP and IL form nano-structures in aqueous media and could cage Cyt c via multiple nonspecific soft interactions. Remarkably, the engineered molecular nano-cages of ATP (5–10 mM), IL (300 mg mL−1), and ATP + IL surrounding Cyt c resulted in 9-to-72-fold higher peroxidase activity than native Cyt c with exceptionally high thermal tolerance (110 °C). The polar interactions with the cardiolipin binding site of Cyt c, mediated by hydrotropes, were well correlated with the increased peroxidase activity. Furthermore, higher activity trends were observed in the presence of urea, GuHCl, and trypsin without any protein degradation. Specific binding of hydrotropes in highly mobile regions of Cyt c (Ω 40–54 residues) and enhanced H-bonding with Lys and Arg offered excellent stability under extreme conditions. Additionally, ATP effectively counteracted reactive oxygen species (ROS)-induced denaturation of Cyt c, which was enhanced by the [Sal] counterpart of IL. Overall, this study explored the robustness of nano-structured hydrotropes to have a higher potential for protein packaging with improved stability and activity under extreme conditions. Thus, the present work highlights a novel strategy for real-time industrial biocatalysis to protect mitochondrial cells from ROS-instigated apoptosis. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development

Author

Kamireddy Kiran, Nandini P. Shetty, Lokesh Veeresh, Priyanka Purushottam Sonbarse, and Giridhar Parvatam

Subjects
0301 basic medicine, Phenylpropanoid, biology, Chemistry, Metabolite, Vanillin, Flavour, food and beverages, 04 agricultural and veterinary sciences, Phenylalanine ammonia-lyase, Environmental Science (miscellaneous), Decalepis hamiltonii, biology.organism_classification, Agricultural and Biological Sciences (miscellaneous), 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Biochemistry, Biosynthesis, Gene expression, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Original Article, Biotechnology
Abstract

To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and their mRNA expression profiles were evaluated using quantitative real-time PCR. Flavour metabolite quantification by HPLC analysis confirmed 10, 170 and 500 µg/g 2-hydroxy-4-methoxy benzaldehyde and 4, 20 and 40 µg/g vanillin in first- (3-month-old plant), second- (18-month-old plant) and third-stage tubers (60-month-old matured plant), respectively. The expression of all four genes phenylalanine ammonia lyase (DhPAL), cinnamate-4-hydroxylase (DhC4H), caffeic acid-O-methyltransferase (DhCOMT) and vanillin synthase (DhVAN) increased with flavour development from first stage to second stage. A decrease in expression from 1.9-folds to 0.1-folds and 19.2-folds to 5.2-folds for DhCOMT and DhVAN was recorded for second stage to third stage, respectively. However, a gradual increase in expression of DhPAL (up to 26.4-folds) and a constant expression pattern for DhC4H (up to 7.1-folds) was evident from second stage to third stage of flavour development. The decrease in the expression levels of DhCOMT and DhVAN in third stage shows that the second-stage tubers are more transcriptionally active towards flavour biosynthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13205-018-1388-7) contains supplementary material, which is available to authorized users.

Published
2018

12. Evaluation of folate-binding proteins and stability of folates in plant foliages

Author

Peethambaran Divya, Bhagyalakshmi Neelwarne, Bijesh Puthusseri, Lokesh Veeresh, and Gyanendra Kumar

Subjects
0106 biological sciences, 0301 basic medicine, Arabidopsis, Coriandrum, Biology, 01 natural sciences, Analytical Chemistry, Metabolic engineering, 03 medical and health sciences, chemistry.chemical_compound, Folic Acid, Gene Expression Regulation, Plant, Gene, Plant Proteins, Folate binding, Microarray analysis techniques, Protein Stability, General Medicine, biology.organism_classification, 030104 developmental biology, chemistry, Biochemistry, Metabolic Engineering, Folate receptor, Salicylic Acid, Salicylic acid, 010606 plant biology & botany, Food Science, Biosynthetic genes
Abstract

The present study reports the presence of folate binding proteins (FBPs) in the plants, coriander and Arabidopsis, and their contributions toward folate enhancement. After observing that salicylic acid (SA) enhanced the accumulation of folates in coriander, a study was conducted in Arabidopsis, where twofold increase in folates occurred in foliage upon SA treatment. For obtaining insights into genes involved in SA-induced folate accumulation, microarray data of responsive genes in Arabidopsis were screened. Based on the expression profiles, 19 genes were further analysed by qPCR. The results revealed that folate biosynthetic genes were largely down-regulated, whereas a gene of a putative folate-binding protein (FPB) was up-regulated, which correlated with a significant increase of FPBs in foliage. This new information on a plant FBP appears useful for metabolic engineering of a wide range of crops to enhance the content and stability of the folates during post-harvest storage.

Published
2017

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