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11 results on '"Lohberger, Andrea"'

1. Widespread bacterial diversity within the bacteriome of fungi

Author

Robinson, Aaron J., House, Geoffrey L., Morales, Demosthenes P., Kelliher, Julia M., Gallegos-Graves, La Verne, LeBrun, Erick S., Davenport, Karen W., Palmieri, Fabio, Lohberger, Andrea, Bregnard, Danaé, Estoppey, Aislinn, Buffi, Matteo, Paul, Christophe, Junier, Thomas, Hervé, Vincent, Cailleau, Guillaume, Lupini, Simone, Nguyen, Hang N., Zheng, Amy O., Gimenes, Luciana Jandelli, Bindschedller, Saskia, Rodrigues, Debora F., Werner, James H., Young, Jamey D., Junier, Pilar, and Chain, Patrick S. G.

Published
2021
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3. Democratization of fungal highway columns as a tool to investigate bacteria associated with soil fungi

Author

Junier, Pilar, primary, Cailleau, Guillaume, additional, Palmieri, Ilona, additional, Vallotton, Celine, additional, Trautschold, Olivia C, additional, Junier, Thomas, additional, Paul, Christophe, additional, Bregnard, Danae, additional, Palmieri, Fabio, additional, Estoppey, Aislinn, additional, Buffi, Matteo, additional, Lohberger, Andrea, additional, Robinson, Aaron, additional, Kelliher, Julia M, additional, Davenport, Karen, additional, House, Geoffrey L, additional, Morales, Demosthenes, additional, Gallegos-Graves, La Verne, additional, Dichosa, Armand E K, additional, Lupini, Simone, additional, Nguyen, Hang N, additional, Young, Jamey D, additional, Rodrigues, Debora F, additional, Parra-Vasquez, A Nicholas G, additional, Bindschedler, Saskia, additional, and Chain, Patrick S G, additional

Published
2021
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6. Identification and antifungal susceptibility of a large collection of yeast strains isolated in Tunisian hospitals

Author

Eddouzi, Jamel, Lohberger, Andrea, Vogne, Christelle, Manai, Mohamed, Sanglard, Dominique, Eddouzi, Jamel, Lohberger, Andrea, Vogne, Christelle, Manai, Mohamed, and Sanglard, Dominique

Abstract

In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a rapid method to identify yeasts isolated from patients in Tunisian hospitals. When identification could not be exstablished with this procedure, sequencing of the internal transcribed spacer with 5.8S ribosomal DNA (rDNA) (ITS1-5.8S-ITS2) and D1/D2 domain of large-subunit (LSU rDNA) were employed as a molecular approach for species differentiation. Candida albicans was the dominant species (43.37% of all cases), followed by C. glabrata (16.55%), C. parapsilosis (13.23%), C. tropicalis (11.34%), C. dubliniensis (4.96%), and other species more rarely encountered in human diseases such as C. krusei, C. metapsilosis, C. lusitaniae, C. kefyr, C. palmioleophila, C. guilliermondii, C. intermedia, C. orthopsilosis, and C. utilis. In addition, other yeast species were obtained including Saccharomyces cerevisiae, Debaryomyces hansenii (anamorph known as C. famata), Hanseniaspora opuntiae, Kodamaea ohmeri, Pichia caribbica (anamorph known as C. fermentati), Trichosporon spp. and finally a novel yeast species, C. tunisiensis. The in vitro antifungal activities of fluconazole and voriconazole were determined by the agar disk diffusion test and Etest, while the susceptibility to additional antifungal agents was determined with the Sensititre YeastOne system. Our results showed low incidence of azole resistance in C. albicans (0.54%), C. tropicalis (2.08%) and C. glabrata (4.28%). In addition, caspofungin was active against most isolates of the collection with the exception of two K. ohmeri isolates. This is the first report to describe caspofungin resistant isolates of this yeast

Published
2017

10. Distinct Roles of Candida albicansDrug Resistance Transcription Factors TAC1, MRR1, and UPC2in Virulence

Author

Lohberger, Andrea, Coste, Alix T., and Sanglard, Dominique

Abstract

ABSTRACTAzoles are widely used in antifungal therapy in medicine. Resistance to azoles can occur in Candida albicansprincipally by overexpression of multidrug transporter gene CDR1, CDR2, or MDR1or by overexpression of ERG11, which encodes the azole target. The expression of these genes is controlled by the transcription factors (TFs) TAC1(involved in the control of CDR1and CDR2), MRR1(involved in the control of MDR1), and UPC2(involved in the control of ERG11). Several gain-of-function (GOF) mutations are present in hyperactive alleles of these TFs, resulting in the overexpression of target genes. While these mutations are beneficial to C. albicanssurvival in the presence of the antifungal drugs, their effects could potentially alter the fitness and virulence of C. albicansin the absence of the selective drug pressure. In this work, the effect of GOF mutations on C. albicansvirulence was addressed in a systemic model of intravenous infection by mouse survival and kidney fungal burden assays. We engineered a set of strains with identical genetic backgrounds in which hyperactive alleles were reintroduced in one or two copies at their genomic loci. The results obtained showed that neither TAC1nor MRR1GOF mutations had a significant effect on C. albicansvirulence. In contrast, the presence of two hyperactive UPC2alleles in C. albicansresulted in a significant decrease in virulence, correlating with diminished kidney colonization compared to that by the wild type. In agreement with the effect on virulence, the decreased fitness of an isolate with UPC2hyperactive alleles was observed in competition experiments with the wild type in vivobut not in vitro. Interestingly, UPC2hyperactivity delayed filamentation of C. albicansafter phagocytosis by murine macrophages, which may at least partially explain the virulence defects. Combining the UPC2GOF mutation with another hyperactive TF did not compensate for the negative effect of UPC2on virulence. In conclusion, among the major TFs involved in azole resistance, only UPC2had a negative impact on virulence and fitness, which may therefore have consequences for the epidemiology of antifungal resistance.

Published
2013
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11. Identification and antifungal susceptibility of a large collection of yeast strains isolated in Tunisian hospitals

Author

Eddouzi, Jamel, Lohberger, Andrea, Vogne, Christelle, Manai, Mohamed, Sanglard, Dominique, Eddouzi, Jamel, Lohberger, Andrea, Vogne, Christelle, Manai, Mohamed, and Sanglard, Dominique

Abstract

In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a rapid method to identify yeasts isolated from patients in Tunisian hospitals. When identification could not be exstablished with this procedure, sequencing of the internal transcribed spacer with 5.8S ribosomal DNA (rDNA) (ITS1-5.8S-ITS2) and D1/D2 domain of large-subunit (LSU rDNA) were employed as a molecular approach for species differentiation. Candida albicans was the dominant species (43.37% of all cases), followed by C. glabrata (16.55%), C. parapsilosis (13.23%), C. tropicalis (11.34%), C. dubliniensis (4.96%), and other species more rarely encountered in human diseases such as C. krusei, C. metapsilosis, C. lusitaniae, C. kefyr, C. palmioleophila, C. guilliermondii, C. intermedia, C. orthopsilosis, and C. utilis. In addition, other yeast species were obtained including Saccharomyces cerevisiae, Debaryomyces hansenii (anamorph known as C. famata), Hanseniaspora opuntiae, Kodamaea ohmeri, Pichia caribbica (anamorph known as C. fermentati), Trichosporon spp. and finally a novel yeast species, C. tunisiensis. The in vitro antifungal activities of fluconazole and voriconazole were determined by the agar disk diffusion test and Etest, while the susceptibility to additional antifungal agents was determined with the Sensititre YeastOne system. Our results showed low incidence of azole resistance in C. albicans (0.54%), C. tropicalis (2.08%) and C. glabrata (4.28%). In addition, caspofungin was active against most isolates of the collection with the exception of two K. ohmeri isolates. This is the first report to describe caspofungin resistant isolates of this yeast

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