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On September 7 and 8, 2022, Healthy Environment and Endocrine Disruptors Strategies, an Environmental Health Sciences program, convened a scientific workshop of relevant stakeholders involved in obesity, toxicology, or obesogen research to review the state of the science regarding the role of obesogenic chemicals that might be contributing to the obesity pandemic. The workshops objectives were to examine the evidence supporting the hypothesis that obesogens contribute to the etiology of human obesity; to discuss opportunities for improved understanding, acceptance, and dissemination of obesogens as contributors to the obesity pandemic; and to consider the need for future research and potential mitigation strategies. This report details the discussions, key areas of agreement, and future opportunities to prevent obesity. The attendees agreed that environmental obesogens are real, significant, and a contributor at some degree to weight gain at the individual level and to the global obesity and metabolic disease pandemic at a societal level; moreover, it is at least, in theory, remediable.

Nearly one fifth of the world's children are experiencing overweight or obesity--that is, an estimated 39 million children younger than five years and 340 million children aged 5-19 years. (1) [...]

Tackling childhood overweight and obesity is critical not only to improve the health and well-being of children and adolescents, but also for entire populations and future generations. This paper provides the latest evidence on the extent of, and risk factors for, childhood overweight and obesity in Vietnam. The landscape analysis tool developed by the United Nations Children’s Fund (UNICEF) and World Health Organization (WHO) was used. A search for peer-reviewed articles in English on online databases was undertaken. Peer-reviewed Vietnamese articles were also retrieved from a range of sources. The prevalence of overweight among children aged under 5 years increased from 5.6% in 2010 to 7.4% in 2019. For overweight and obesity among children aged 5 to 19 years, prevalence rose from 8.5% and 2.5% in 2010 to 19% and 8.1% in 2020, respectively. Maternal malnutrition, gestational diabetes during pregnancy, and inadequate infant and young child feeding practices are all risk factors for early childhood overweight. Unhealthy diets, insufficient physical activity, and lack of sleep are among the risk factors for overweight and obesity among school aged children and adolescents. The prevention of overweight and obesity among Vietnamese children requires a whole-of-government, cross-sectoral approach to addresses the obesogenic environment that is negatively influencing the nutrition of children.

Efforts to prevent and treat obesity need to be grounded in science. A historical focus on individual responsibility has been ineffective in halting the rise in obesity prevalence. There needs to be a better understanding of environmental and biological drivers of weight gain to help reduce weight bias and stigma and identify more effective policies for action.

Summary Obesity prevention policies are a priority for many governments and intergovernmental agencies. Policy makers not only use systematic reviews of effectiveness but also consider contextual issues including cost and cost‐effectiveness, equity, rights, acceptability and feasibility. To support their work, the present narrative review examines three contextual issues (costs, equity and acceptability) in relation to three policies for obesity prevention: sweetened beverage taxes, front‐of‐pack nutrition labelling and restrictions on advertising to children. Literature searches led to over 1100 documents, of which 125 informed the present review. Beverage taxes were found likely to be highly cost‐effective, moderately favourable for health equity, supported by the public (depending on the use of revenues) and by health professionals and civil society groups and opposed by commercial interests. Depending on the design, front‐of‐pack nutritional labelling is likely to be highly cost‐effective, moderately favourable for health equity, supported by the public, health professionals and civil society groups, and opposed by commercial interests. Restrictions on child‐directed advertising are likely to be highly cost‐effective in the longer term, moderately favourable for health equity, supported by the public, health professionals and civil society groups and opposed by commercial interests (unless voluntary). The evidence base needs strengthening, but the authors find that all three policies merit consideration by governmental authorities, and should be implemented to reduce obesity risk.

Let G = (V, E) be a finite graph and r ≥ 1 be an integer. For v ∈ V, let Br(v) = {x ∈ V : d(v, x) ≤ r} be the ball of radius r centered at v. A set C ⊆ V is an r-dominating code if for all v ∈ V, we have Br(v) ∩ C ≠ ∅; it is an r-locating-dominating code if for all v ∈ V, we have Br(v) ∩ C ≠ ∅, and for any two distinct non-codewords x ∈ V \ C, y ∈ V \ C, we have Br(x) ∩ C ≠ Br(y) ∩ C; it is an r-identifying code if for all v ∈ V, we have Br(v) ∩ C ≠ ∅, and for any two distinct vertices x ∈ V, y ∈ V, we have Br(x) ∩ C ≠ Br(y) ∩ C. We denote by γr(G) (respectively, ldr(G) and idr(G)) the smallest possible cardinality of an r-dominating code (respectively, an r-locating-dominating code and an r-identifying code). We study how small and how large the three differences idr(G)−ldr(G), idr(G)−γr(G) and ldr(G) − γr(G) can be.

Abstract Background Production of correctly disulfide bonded proteins to high yields remains a challenge. Recombinant protein expression in Escherichia coli is the popular choice, especially within the research community. While there is an ever growing demand for new expression strains, few strains are dedicated to post-translational modifications, such as disulfide bond formation. Thus, new protein expression strains must be engineered and the parameters involved in producing disulfide bonded proteins must be understood. Results We have engineered a new E. coli protein expression strain named SHuffle, dedicated to producing correctly disulfide bonded active proteins to high yields within its cytoplasm. This strain is based on the trxB gor suppressor strain SMG96 where its cytoplasmic reductive pathways have been diminished, allowing for the formation of disulfide bonds in the cytoplasm. We have further engineered a major improvement by integrating into its chromosome a signal sequenceless disulfide bond isomerase, DsbC. We probed the redox state of DsbC in the oxidizing cytoplasm and evaluated its role in assisting the formation of correctly folded multi-disulfide bonded proteins. We optimized protein expression conditions, varying temperature, induction conditions, strain background and the co-expression of various helper proteins. We found that temperature has the biggest impact on improving yields and that the E. coli B strain background of this strain was superior to the K12 version. We also discovered that auto-expression of substrate target proteins using this strain resulted in higher yields of active pure protein. Finally, we found that co-expression of mutant thioredoxins and PDI homologs improved yields of various substrate proteins. Conclusions This work is the first extensive characterization of the trxB gor suppressor strain. The results presented should help researchers design the appropriate protein expression conditions using SHuffle strains.