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145 results on '"Lobasso, Simona"'

4. Prefrontal dysfunction in post-COVID-19 hyposmia: an EEG/fNIRS study

Author

Clemente, Livio, primary, La Rocca, Marianna, additional, Quaranta, Nicola, additional, Iannuzzi, Lucia, additional, Vecchio, Eleonora, additional, Brunetti, Antonio, additional, Gentile, Eleonora, additional, Dibattista, Michele, additional, Lobasso, Simona, additional, Bevilacqua, Vitoantonio, additional, Stramaglia, Sebastiano, additional, and de Tommaso, Marina, additional

Published
2023
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18. Use of MALDI-TOF mass spectrometry to reveal oxidized cardiolipin species

Author

Lobasso, Simona, Angelini, Roberto, Gorgoglione, Ruggiero, Lopalco, Patrizia, Belfiore, Anna, and Corcelli, Angela

Subjects
lipids (amino acids, peptides, and proteins)
Abstract

Detection, identification, characterization and quantitation of oxidized cardiolipin forms in vivo is considered a challenging task, because these lipid species exist in low abundance in the biological systems. Recently it has been shown that chain-shortened products of oxidized standard phosphatidylcholines could be successfully identified by matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) (1). Here we have characterized oxidation products of standard tetra-linoleoyl cardiolipin, such as hydroxide- and hydroperoxide-derivatives and truncated forms, by MALDI-TOF/MS analysis. In addition, by following a recently developed protocol (2), we have analysed MALDI/TOF cardiolipin fingerprints of intact leukocytes isolated from controls and aged chronic heart failure patients, in order to check for the presence of oxidized cardiolipin species. The comparison of the two sets of analyses has showed MALDI signals potentially attributable to oxidized cardiolipin species in the mass spectra of patients. In conclusion, the use of MALDI-TOF/MS analyses on intact cells or organelles might offer novel opportunities to detect oxidized lipid species in native biomembranes., Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 3 (2016)

Published
2016
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20. Lipid profiling of parkin-mutant human skin fibroblasts.

Author

Lobasso, Simona, Tanzarella, Paola, Vergara, Daniele, Maffia, Michele, Cocco, Tiziana, and Corcelli, Angela

Subjects
*PARKINSON'S disease, *MITOCHONDRIA, *PARKIN (Protein), *AUTOPHAGY, *CELL physiology, *FIBROBLASTS
Abstract

Parkin mutations are a major cause of early-onset Parkinson's disease (PD). The impairment of protein quality control system together with defects in mitochondria and autophagy process are consequences of the lack of parkin, which leads to neurodegeneration. Little is known about the role of lipids in these alterations of cell functions. In the present study, parkin-mutant human skin primary fibroblasts have been considered as cellular model of PD to investigate on possible lipid alterations associated with the lack of parkin protein. Dermal fibroblasts were obtained from two unrelated PD patients with different parkin mutations and their lipid compositions were compared with that of two control fibroblasts. The lipid extracts of fibroblasts have been analyzed by combined matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) and thin-layer chromatography (TLC). In parallel, we have performed direct MALDI-TOF/MS lipid analyses of intact fibroblasts by skipping lipid extraction steps. Results show that the proportions of some phospholipids and glycosphingolipids were altered in the lipid profiles of parkin-mutant fibroblasts. The detected higher level of gangliosides, phosphatidylinositol, and phosphatidylserine could be linked to dysfunction of autophagy and mitochondrial turnover; in addition, the lysophosphatidylcholine increase could represent the marker of neuroinflammatory state, a well-known component of PD. [ABSTRACT FROM AUTHOR]

Published
2017
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21. Mammalian olfactory receptors : GPCRs exhibiting a functionality outside lipid rafts

Author

Sanz, Guenhaël, Persuy, Marie-Annick, Vidic, Jasmina, Wade, Fallou, Péchoux, Christine, Chat, Sophie, Lobasso, Simona, Angelini, R, Monnerie, Regine, Corcelli, Angela, Pajot-Augy, Edith, Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche 144 (NeuroSud Paris) (IFR 144), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Unité de recherche génomique et physiologie de la lactation (GPL), and Università degli Studi di Bari Aldo Moro

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2010

27. Localisation des récepteurs olfactifs exprimés de façon hétérologue dans la levure S. cerevisiae

Author

Sanz, Guenhaël, Persuy, Marie-Annick, Vidic, Jasmina, Wade, Serigne Fallou, Longin, Christine, Corcelli, Angela, Lobasso, Simona, Monnerie, Regine, Pajot-Augy, Edith, Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche 144 (NeuroSud Paris) (IFR 144), Unité de recherche génomique et physiologie de la lactation (GPL), and Università degli Studi di Bari Aldo Moro

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2009

35. Role of palmitic acid on isolation and properties of halorhodopsin

Author

Corcelli Angela, Lobasso Simona, Lopez Francesco, Palmisano Francesco, Guerrieri Antonio, and Trotta Massimo

Subjects
Haloropsin, retinal protein
Abstract

Halorhodopsin, a photoactivated Cl- pump of halobacteria, shows several analogies with bovine rhodopsin. As it is known that bovine rhodopsin is acylated, we have examined the possibility that fatty acids ore covalently bound to halorhodopsin too. Purified holorhodopsin was isolated from Halobacterium halobium as previously described (Duschl Aet ol, 263:17016-17022, 1988). Two purple fractions were eluted from phenylsepharose column; the absorption spectra of the two holorhodopsin fractions in the dark were identical. The two fractions showed the same 20 kDa protein bond on SDS-PAGE (Blonck A, Oesterhelt D, 6: 265-273, 2987). Lipids released by alkaline hydrolysis of the two halorhodopsin fractions were esterified with HCl/MeOH, analysed by gas chromutogruphy/mass spectrometry and quantitated using an internal standard. We have identified methyl palmitute among derivotized lipids released from both halorhodopsin fractions; the paImitate content of the first eluted fraction wus higher than the second. By udding palmitote to buffers used in the phenylsephorose chromatography, only one sharp purple bond was collected. The halorhodopsin photoreaction, studied by an optical multichunnel analyzer, was found to be dependent on the amount of polmitate associated to purified halorhodopsin.

Published
1995

48. Relationship between cardiolipin metabolism and oxygen availability in Bacillus subtilis.

Author

Lobasso, Simona, Palese, Luigi L., Angelini, Roberto, and Corcelli, Angela

Subjects
CARDIOLIPIN, METABOLISM, BACILLUS subtilis, OXYGEN, PHOSPHOLIPIDS, HYPOXEMIA, CYANIDES
Abstract

Abstract: We report changes of the content of anionic phospholipids in Bacillus subtilis in response to hypoxic conditions and inhibition of terminal respiration. Cardiolipin accumulates rapidly when bacteria are suspended in non-growth medium under reduced aeration or exposed to the inhibitor cyanide; the increase of cardiolipin occurs at the expense of its precursor phosphatidylglycerol and is temperature-dependent. Depending on the extent of hypoxic stress, membranes containing different levels of cardiolipin can be isolated from B. subtilis cells. The NADH oxidase activity in cardiolipin-enriched membranes is cyanide-resistant; furthermore O2 consumption measurements indicated that cardiolipin-enriched cells are resistant to cyanide. Results point out a possible interdependence between the effect of cyanide on cardiolipin metabolism and the effect of cardiolipin on the effectiveness of cyanide inhibition. [Copyright &y& Elsevier]

Published
2013
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