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Statistical bias correction (BC) is a widely used tool to post-process climate model biases in heat-stress impact studies, which are often based on the indices calculated from multiple dependent variables. This study compares four BC methods (three univariate and one multivariate) with two correction strategies (direct and indirect) for adjusting two heat-stress indices with different dependencies on temperature and relative humidity using multiple regional climate model simulations over South Korea. It would be helpful for reducing the ambiguity involved in the practical application of BC for climate modeling and end-user communities. Our results demonstrate that the multivariate approach can improve the corrected inter-variable dependence, which benefits the indirect correction of heat-stress indices depending on the adjustment of individual components, especially those indices relying equally on multiple drivers. On the other hand, the direct correction of multivariate indices using the quantile delta mapping univariate approach can also produce a comparable performance in the corrected heat-stress indices. However, our results also indicate that attention should be paid to the non-stationarity of bias brought by climate sensitivity in the modeled data, which may affect the bias-corrected results unsystematically. Careful interpretation of the correction process is required for an accurate heat-stress impact assessment.

How the added value of dynamically downscaled climate variables can be transferable to the hydrological impact assessment has been a long standing issue. This study investigates the potential benefit of highresolution climate data locally tailored over South Korea in terms of the reduction of uncertainties in hydrological projections. For this purpose, a large ensemble consisting of three Global Climate Model (GCM) projections and their downscaling products with different resolutions (i.e., 20 and 5 km), and four bias correction (BC) methods is fed into a semi-distributed hydrological model (HM) customized over Korean river basins. The in-depth comparison among the 45-members hydrological simulations proves that the added value of RCM would not be erased by the application of BC. While this study acknowledges the necessity of BC to remove the systematic bias in climate simulations, it is found that the high-resolution dynamical downscaling can significantly narrow the spread brought with different BC methods, thus reducing the uncertainty in the projected hydrological change. The projected streamflow changes for both the mean of wet season and the high flows indicate that there will be an intensified runoff, especially for the extremes, over South Korea under the warming. Altogether, this study provides a valuable exploration of uncertainty reduction in hydrological projections from the perspective of resolution effect of dynamical downscaling, which is meaningful for hydroclimate studies and climate change impact assessment.

Chemotherapic cancer treatment has suffered from side effects and poor target specificity. pH-triggered drug delivery systems offer the opportunity to better deliver anticancer drugs to cancer cells in a more controllable manner. Herein, we reported a facile method to synthesize Mg-doped Ca2SiO4 (CaMgSiO4) nanospheres with highly porous structures as pH-responsive drug delivery. Material characterizations confirmed the uniform Mg doping within calcium silicates. After the Mg doping, the surface area of calcium silicates increased from 185.68 m2/g to 212.71 m2/g. When incorporated with PEG surface coating, the porous CaMgSiO4 nanoparticles displayed over 50% increased doxorubicin (DOX) loading capacity compared with Ca2SiO4 nanoparticles. The pH-triggered DOX release behaviors and cytotoxicity of porous CaMgSiO4 nanoparticles were investigated. As a result, porous CaMgSiO4 nanoparticles were highly sensitive to pH stimulus and exhibited high biocompatibility and strong cancer cell killing effect (over 75%).

In this work, a reasonable strategy for the construction of Li2ZnTi3O8@Na2WO4 composite was employed to promote the Li storage performances of Li2ZnTi3O8. The Li2ZnTi3O8@Na2WO4 composites (5, 10, and 15 wt%) were then prepared by a solution dispersion method. The introduction of Na2WO4 does not change the structures of the samples and they show similar morphologies with particle sizes from 100 to 200 nm. Suitable amount of Na2WO4 modification effectively improves the electrochemical performance of Li2ZnTi3O8. Li2ZnTi3O8@Na2WO4 composites (0, 5, 10, and 15 wt%) deliver the discharge/charge capacities of 137.4/136.4, 164.2/162.3, 189.2/188.1, and 154.5/153.3 mAh g−1 at 0.5 A g−1 after 100 cycles, respectively. Li2ZnTi3O8@Na2WO4 composites (10 wt%) has the highest reversible capacities among all samples. The Na2WO4 shell with an excellent electronic conductivity can reduce electrode polarization, decrease the charge transfer resistance, enhance the Li-ion diffusion coefficient of Li2ZnTi3O8, and then improve the electrochemical kinetics of composites. In addition, the formation of Ti–O bonds at the interface can be helpful for the stabilization of the composite, being beneficial for the improvement of their cycling stabilities. These results reveal that Na2WO4 coating is a facile and effective strategy to promote the Li storage performance of Li2ZnTi3O8.

Statistical bias correction (BC) is a widely used tool to post-process climate model biases for heat-stress impact studies, which are often based on indices calculated from multiple dependent variables. This study compares five bias correction methods (four univariate and one multivariate) with two applying strategies (direct and indirect) for correcting two heat-stress indices with different dependencies on temperature and relative humidity, using multiple Regional Climate Model simulations over South Korea. It would be helpful for reducing the ambiguity involved in the practical application of BC for climate modeling as well as end-user communities. Our results demonstrate that the multivariate approach can improve the corrected inter-variable dependence and therefore benefit the indirect correction of heat-stress indices that depend on the adjustment of individual components, especially those relying equally on multiple drivers. On the other hand, the direct correction of multivariate indices using the Quantile Delta Mapping univariate approach can also produce a comparable performance in the corrected heat-stress indices. However, our results also indicate that attention should be paid to the non-stationarity of bias brought by climate sensitivity in the modeled data, which may affect the bias-corrected results unsystematically. Careful interpretation of the correction process is required for an accurate heat-stress impact assessment.

Chronic wounds have become an important factor hindering human health, affecting tens of millions of people worldwide, especially diabetic wounds. Based on the antibacterial properties of chitosan, the angiogenesis promoting effect of vaccarin (VAC) and the anti-inflammatory effect of hypaphorine (HYP), nanoparticles with high bioavailability were prepared. VAC, HYP and chitosan nanoparticles (VAC + HYP-NPS) were used to the treatment of chronic wounds. Transmission electron microscopy (TEM) images showed the nanoparticles were spherical. ZetaPALS showed the potential of nanoparticles were -12.8 ± 5.53 mV and the size were 166.8 ± 29.95 nm. Methyl thiazolyl tetrazolium (MTT) assay showed that VAC + HYP-NPS had no toxicity and the biocompatibility was satisfactory. In the treatment of chronic wounds in diabetic rats, VAC + HYP-NPS significantly promoted the re-epithelialization of chronic wounds and accelerated the healing of chronic wounds. In the process of chronic wounds healing, VAC + HYP-NPS played the antibacterial effect of chitosan, the angiogenic effect of VAC and the anti-inflammatory effect of HYP, and finally promoted the chronic wounds healing. Overall, the developed VAC + HYP-NPS have potential application in chronic wounds healing. In view of the complexity of the causes of chronic wounds, multi-target drug administration may be an effective way to treat chronic wounds.

Wound healing is a complex, dynamic and difficult process. Much effort and attempt has been made to accelerate this process. The purpose of this study is to prepare nanoparticles loaded with vaccarin (VAC-NPS)hydrogel and evaluate its effect on promoting wound healing. In the present study, the physicochemical properties of VAC-NPS were characterized. Transmission electron microscopy (TEM) was used to observe the morphology of VAC-NPS. Human umbilical vein endothelial cells (HUVEC) was employed to assessment the biocompatibility of VAC-NPS in vitro. The wound healing function of VAC-NPS hydrogels was evaluated in the full-thickness dermal wound in a rat model. The results indicated that VAC-NPS was spherical like particles with uniform particle size distribution and no obvious aggregation with a diameter of (216.6 ± 10.1)nm. The loading capacity and encapsulation efficiency of VAC in the nanoparticles were (14.3 ± 1.2) % and (51.7 ± 1.7) % respectively. MTT assay demonstrated that the VAC-NPS had no cytotoxicity and could promote HUVEC proliferation and migration. In vivo results showed that VAC-NPS promotes wound healing, and the mechanism may be through up-regulating IL-1β and PDGF-BB, promoting angiogenesis. VAC-NPS might have a potential application value for the treatment of the wound healing and a promising performance in bio-medically relevant systems.

The information of when and where region-specific patterns in both mean and extreme temperatures leading to heat stress will emerge from the present-day climate variability is important to plan adaptation options, but to date studies on this issue still remain limited and fragmented. Here, we estimate the time of emergence (TOE) of temperature and wet-bulb temperature (Tw), a better indication of heat stress, using fine-scale, long-term regional climate model projections under the RCP2.6 and RCP8.5 scenarios across six different domains. Differently from previous studies, the TOE is determined using three methods applied on impact-relevant variables: two different signal-to-noise frameworks based on summer mean temperature and Tw and a statistical test to identify significant differences in daily extreme distributions. The TOE response to RCP2.6 and RCP8.5 with respect to the end of 20th century variability differs significantly regardless of which TOE metric is applied. For summer mean temperature, the land fraction reaching TOE is expected to exceed 90% by the 2050s under the RCP8.5, whereas the increase rate of land exposure to TOE tends to stagnate over time under the RCP2.6 so that more than 40% of land will not experience TOE by the end of the 21st century. Compared to temperature, the TOE of Tw is reached earlier in most of the wet tropics but is delayed in hot and dry regions because of the nonlinear response of Tw to humidity. For both temperature and Tw, the TOE appears earlier in regions with low baseline variability, such as in the tropics. Despite the uncertainties arising from the choice of TOE metrics, the vast majority of regions in Africa and southeast Asia experience TOE in the early 21st century under both the RCP2.6 and RCP8.5 scenarios, which stresses the urgent need for developing adequate adaptation strategies in these regions.

Lung cancer always ranks first in the number of cancer deaths every year, accounting for 18.4% of total cancer deaths in 2018. Metastasis is the main cause of death in lung cancer patients. The identification of bioactive components of traditional Chinese medicine is very important for the development of novel reagents against non-small cell lung cancer (NSCLC). Rosthorin A has originated from Rabdosia rosthornii (Diels) Hara which excerpts from 'Chinese materia medica', and is known to have 'clear heat phlegm' properties in the folk. Little is known about the biological functions and mechanisms of Rosthorin A in cancer cells at present. The role of EMT in metastasis of a tumor cell is self-evident. Slug is an important EMT inducer, which is related to the development of lung cancer. Cell growth, clone assay, cell migration, cell invasion, and protein expression, and NSCLC transplanted tumor growth were performed in A549, H1299, and H1975 cells. Rosthorin A significantly inhibited the growth of NSCLC cells, it could prolong the survival of nude mice. Rosthorin A inhibited the migration and invasion of A549, H1299, and H1975 cells. Rosthorin A up-regulated E-cadherin expression level and down-regulated the expression of β-catenin, N-cadherin, vimentin, Slug, and Twist. Rosthorin A could promote the expression of E-cadherin and inhibit the development of EMT by downregulating Slug, to inhibit the development and metastasis of NSCLC cells. In summary, Rosthorin A could be used as a promising candidate for the treatment of NSCLC patients with recurrence and metastasis.

In this work, a rational design and construction of porous spherical NiO@NiMoO4 wrapped with PPy was reported for the application of high-performance supercapacitor (SC). The results show that the NiMoO4 modification changes the morphology of NiO, and the hollow internal morphology combined with porous outer shell of NiO@NiMoO4 and NiO@NiMoO4@PPy hybrids shows an increased specific surface area (SSA), and then promotes the transfer of ions and electrons. The shell of NiMoO4 and PPy with high electronic conductivity decreases the charge-transfer reaction resistance of NiO, and then improves the electrochemical kinetics of NiO. At 20 A g−1, the initial capacitances of NiO, NiMoO4, NiO@NiMoO4 and NiO@NiMoO4@PPy are 456.0, 803.2, 764.4 and 941.6 F g−1, respectively. After 10,000 cycles, the corresponding capacitances are 346.8, 510.8, 641.2 and 904.8 F g−1, respectively. Especially, the initial capacitance of NiO@NiMoO4@PPy is 850.2 F g−1, and remains 655.2 F g−1 with a high retention of 77.1% at 30 A g−1 even after 30,000 cycles. The calculation result based on density function theory shows that the much stronger Mo-O bonds are crucial for stabilizing the NiO@NiMoO4 composite, resulting in a good cycling stability of these materials.

Chronic wound repair is a common complication in patients with diabetes mellitus, which causes a heavy burden on social medical resources and the economy. Hypaphorine (HYP) has good anti-inflammatory effect, and chitosan (CS) is used in the treatment of wounds because of its good antibacterial effect. The purpose of this research was to investigate the role and mechanism of HYP-nano-microspheres in the treatment of wounds for diabetic rats. The morphology of HYP-NPS was observed by transmission electron microscopy (TEM). RAW 264.7 macrophages were used to assess the bio-compatibility of HYP-NPS. A full-thickness dermal wound in a diabetic rat model was performed to evaluate the wound healing function of HYP-NPS. The results revealed that HYP-NPS nanoparticles were spherical with an average diameter of approximately 50 nm. The cell experiments hinted that HYP-NPS had the potential as a trauma material. The wound test in diabetic rats indicated that HYP-NPS fostered the healing of chronic wounds. The mechanism was through down-regulating the expression of pro-inflammatory cytokines IL-1β and TNF-α in the skin of the wound, and accelerating the transition of chronic wound from inflammation to tissue regeneration. These results indicate that HYP-NPS has a good application prospect in the treatment of chronic wounds.

This study investigates the added value of very high resolution in long-term climate simulations over South Korea using the Weather Research and Forecasting (WRF) model. A one-way double-nested modeling system consisting of a mother domain (20-km resolution) and nested domain (5-km resolution) is customized for simulating the distinct climatological patterns in Korea, where the region-specific climate is largely influenced by the area’s complex geographical features. The ERA-Interim reanalysis data are used for the initial and boundary conditions, and the simulation spans the period from December 1, 1985 to December 31, 2005 (20-year analysis period with 1-month spin-up). Simulations from both the mother and the nested domain show reasonable performance in capturing the general characteristics of summer temperature and precipitation in terms of temporally and spatially averaged quantities. However, the added value from the nested domain with its higher resolution is apparently found in the reproduction of the intensity and frequency of extreme events and in the physical realism related to the partitioning of convective and large-scale precipitation. The nested domain not only better resolves the sharp gradients of temperature variation over short distances but also substantially reduces the systematic cold bias seen in temperature extremes produced by the mother domain. Furthermore, the nested domain is better able to simulate the upper tail of precipitation distributions and thus of extreme events. The higher resolution also improves the simulation of partitioning between convective and large-scale precipitation, leading to a plausible relationship between extreme precipitation and temperature and showing good agreement with in situ observation. Given the different behaviors of convective and large-scale precipitation in response to temperature changes, their realistic partitioning in the model has important potential for enhancing the reliability of precipitation projection under global warming.

Trichinellosis is a severe zoonosis involving the activation of inflammatory cells, accompanied by the prominent expressions of proinflammatory cytokines in the host. Semen vaccariae, the seeds of Vaccaria segetalis (Neck.) Garcke. ex Asch. (Caryophyllaceae), is a famous traditional herb that is rich in vaccaria n-butanol extract (VNE). Vaccarin is one major active component of VNE, and it is reported in the treatment of stranguria disease. Hypaphorine is another main active component of VNE and has good anti-inflammatory effect, whereas the potential bioactivity of VNE in trichinellosis treatment is still unknown. This study was designed to evaluate the potential anthelmintic and anti-inflammatory activity of VNE toward T. spiralis infection. ICR mice were used to assess the effect of VNE on repression larvae and adult worms in vivo. Immunohistochemistry analysis was performed to evaluate the expression levels of IL-1β, IL-6, TNF-α, and COX-2. Our results showed that VNE could effectively depress the expressions of proinflammatory cytokines, including IL-1β, IL-6, TNF-α, and COX-2. The adult worms were decreased by 79.53%, while the muscle larvae were diminished by 77.70% as compared to the control. These results demonstrated that VNE may be a promising therapeutic agent against the inflammation and diseases caused by T. spiralis infection.

Destructive glucose and lipid metabolism in the liver is a crucial characteristic of type 2 diabetes mellitus (T2DM), eventually leading to insulin resistance and subsequent hyperglycemia and hyperlipidemia. Vaccarin is an active flavonoid glycoside associated with various biological functions, but its effects on glucose and lipid metabolic disorder in T2DM are still unclear. In this study, glucosamine (GlcN) and free fatty acids (FFAs, oleate/palmitate, 2:1 ratio) were applied to mimic insulin resistance and lipid deposition in HepG2 cells, respectively. Type 2 diabetic mice were induced using a high-fat diet (HFD) together with streptozotocin (STZ). GlcN stimulation was found to upregulate glucose production and gluconeogenesis but downregulate glycogen synthesis and phosphorylation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) in HepG2 cells, abnormal changes that were reversed by vaccarin. Furthermore, lipid accumulation was enhanced, but AMPK was inactivated in FFAs-exposed HepG2 cells, issues that were rectified by vaccarin treatment. These ameliorative effects of vaccarin on insulin resistance and steatosis were eliminated by AMPK inhibitor Compound C. In type 2 diabetic mice, chronic vaccarin administration decreased fasting blood glucose and lipid levels in both serum and the liver, and improved insulin sensitivity and steatosis in the liver. Taken together, these results suggest that vaccarin attenuates insulin resistance and steatosis by activating the AMPK signaling pathway.

The original version of the article contained error in the Fig. 8 caption was swapped and below is the corrected caption.

To investigate the efficacy of the extract from Yiyuan Yiliu Tang (, YYYLT) on human lung adenocarcinoma cells A549 and human hepatoma cells Bel7402.The cancer cell lines were treated with various concentrations (0, 100, 200, 300, and 400 μg/mL) of the crude water extract of YYYLT and then cell viability, toxicity, cytokine secretion, and cell cycle/apoptosis were determined by MTT assay, LDH assay, and flow cytometry, respectively.The extract from YYYLT significantly suppressed the proliferation of the cancer cell lines and the release of interleukin-2 and tumor necrosis factor-α in a dose-dependent manner. The extract also promoted apoptosis, caused cell cycle arrest at G0/G1 phase, and increased the expression of caspase-3, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X proteins.The extract from YYYLT might be a potential treatment for human lung and liver cancers.

Introduction Toxoplasma gondii (T. gondii), a parasitic protozoa that is associated with various psychiatric disorders. Both T. gondii infection and disturbed host's lipid profile are common in schizophrenia patients. However, the underlying pathophysiological mechanisms remain speculative. Also, the characteristics of serum lipid levels in schizophrenia patients comorbid with T. gondii infection are not clear. Therefore, it is necessary to explore the influence of chronic T. gondii infection on the characteristic physiological indexes of schizophrenia patients so as to provide some insights into finding target therapeutic drugs. Methods In this study, the effect of chronic T. gondii infection on serum lipid profile was retrospectively analysed in 1719 schizophrenic patients and 1552 healthy subjects from Eastern China. Results The overall prevalence of Immunoglobulin G (IgG) antibodies against T. gondii (17.98%) in schizophrenia patients was significantly higher than healthy controls (7.35%, χ2 = 81.831, P = 0.000). Compared to T. gondii IgG-seronegative schizophrenia patients, IgG-seropositive group had higher high-density lipoprotein (HDL) (P = 0.000) and triglycerides (TG) (P = 0.000) levels, while total cholesterol (TC) (P = 0.000) levels showed an opposite tendency in IgG-seropositive cases. We also found significant correlation between T. gondii seropositivity and increased TG (P = 0.000) and TC levels (P = 0.000) in schizophrenia patients. Binary regression analysis also showed that decreased TC level was more common among schizophrenia patients with T. gondii seropositivity compared to seronegative subjects (OR = 0.617, 95%CI = 0.509–0.749, P = 0.000). Conclusion Patients with chronic T. gondii infection and comorbid schizophrenia had higher HDL and TG levels, while cholesterol levels showed an opposite trend. To the best of our knowledge, this is the first report focus on the host's lipid profile of chronic T. gondii infection and comorbid schizophrenia patients.

Background Mounting evidence suggested a complex correlation between host lipid metabolism and Toxoplasma gondii (T. gondii) infection. However, the inherent association between T. gondii infection and host lipid state remains elusive either in mice or in human hosts. Methods Through a study in a sample of 1045 healthy participants from eastern China, we determined the association of T. gondii infection and host lipid levels using serological methods. We then examined the host lipid levels in C57BL/6 J mice at both acute and chronic T. gondii infection stages (for a period up to 36 weeks post infection). Results In our case-control study, T. gondii seropositive individuals had higher low-density lipoproteins (LDL) (P = 0.0043) and total cholesterol (TC) (P = 0.0134) levels compared to seronegative individuals. Furthermore, individuals with LDL (OR = 3.25; 95% CI:1.60–6.61) and TC (OR = 2.10; 95% CI:1.22–3.63) levels above the upper limit of normal range had higher odds ratio to be T. gondii IgG positive. Consistently, in vivo data revealed that a significantly increased LDL level was first observed at early acute stage but plateaued to later time (chronic infection with T. gondii). Conclusions In both healthy population and T. gondii-infected mice, seropositive individuals had higher LDL level. Individuals with positive T. gondii IgG had more odds of being with LDL and TC abnormality. Latent T. gondii infection is common worldwide, potential medical interventions to host lipid metabolism may be a breakthrough point to the prevention and control of this parasite infection.

Schistosomiasis is an immunopathogenic disease in which a T helper (Th) cell type 2-like response plays vital roles. Hepatic fibrosis is its main pathologic manifestations, which is the leading cause of hepatic cirrhosis. Co-infections of Schistosoma japonicum (Sj) with other pathogens are frequently encountered but are easily ignored in clinical studies, and effective therapeutic interventions are lacking. In this study, we explored the effect of Toxoplasma gondii (Tg) prior infection on Th1/Th2 response, community shifts in gut microbiome (GM), and the pathogenesis of schistosomiasis in murine hosts. Mice were prior infected with Tg before Sj infection. The effects of co-infection on Th1/Th2 response and hepatic fibrosis were analyzed. Furthermore, we investigated this issue by sequencing 16S rRNA from fecal specimens to define the GM profiles during co-infection. Tg prior infection markedly reduced the granuloma size and collagen deposit in livers against Sj infection. Prior infection promoted a shift toward Th1 immune response instead of Th2. Furthermore, Tg infection promoted the expansion of preponderant flora and Clostridiaceae was identified as a feature marker in the GM of the co-infection group. Redundancy analysis (RDA)/canonical correspondence analysis (CCA) results showed that liver fibrosis, Th1/Th2 cytokines were significantly correlated (P &lt, 0.05) with the GM compositions. Tg infection inhibits hepatic fibrosis by downregulating Th2 immune response against Sj infection, and further promotes the GM shifts through &ldquo, gut&ndash, liver axis&rdquo, in the murine hosts. Our study may provide insights into potential anti-fibrosis strategies in co-infection individuals.

Background: Diabetes mellitus is a growing global health issue nearly across the world. Diabetic patients who are prone to develop diabetes-related complications often exhibit progressive neuropathy (painless and sensory loss). It is usual for small wounds to progress to ulceration, which especially worsens with peripheral arterial disease and in the presence of anaerobic bacteria, culminating into gangrene. In our study, vaccarin (VAC), the main active monomer extracted from Chinese herb vaccariae semen, is proven to have a role in promoting diabetic chronic wound healing through a cytoprotective role under high glucose conditions. Materials and methods: We constructed a pressure ulcer on both VAC-treated and control mice based on a type 1 diabetes (T1DM) model. The wound healing index was evaluated by an experimental wound assessment tool (EWAT). We also determined the effect of VAC on the proliferation and cell migration of human microvascular endothelial cells (HMEC-1) by a cell counting kit (CCK-8), a scratch and transwell assay. Results: The results demonstrated that VAC could promote the proliferation and migration of high glucose-stimulated HMEC-1 cells, which depend on the activation of FOXP2/AGGF1. Activation of the angiogenic factor with G patch and FHA domains 1 (AGGF1) caused enhanced phosphorylation of serine/threonine kinase (Akt) and extracellular regulated protein kinases (Erk1/2). By silencing the expression of forkhead box p2 (FOXP2) protein by siRNA, both mRNA and protein expression of AGGF1 were downregulated, leading to a decreased proliferation and migration of HMEC-1 cells. In addition, a diabetic chronic wound model in vivo unveiled that VAC had a positive effect on chronic wound healing, which involved the activation of the above-mentioned pathways. Conclusions: In summary, our study found that VAC promoted chronic wound healing in T1DM mice by activating the FOXP2/AGGF1 pathway, indicating that VAC may be a promising candidate for the treatment of the chronic wounds of diabetic patients.

This study investigates the performance of the latest version of RegCM4 in simulating summer precipitation over South Korea, comparing nine sensitivity experiments with different combinations of convective parameterization schemes (CPSs) between land and ocean. In addition to the gross pattern of seasonal and monthly mean precipitation, the northward propagation of the intense precipitation band and statistics from extreme daily precipitation are thoroughly evaluated against gridded and in situ station observations. The comparative analysis of 10-year simulations demonstrates that no CPS shows superiority in both quantitative and qualitative aspects. Furthermore, a nontrivial discrepancy among the different observation datasets makes a robust assessment of model performance difficult. Regardless of the CPS over the ocean, the simulations with the Kain–Fritsch scheme over land show a severe dry bias, whereas the simulations with the Tiedtke scheme over land suffer from a limited accuracy in reproducing spatial distributions due to the excessive orographic precipitation. In general, the simulations with the Emanuel scheme over land are better at capturing the major characteristics of summer precipitation over South Korea, despite not all statistical metrics showing the best performance. When applying the Emanuel scheme to both land and the ocean, precipitation tends to be slightly overestimated. This deficiency can be alleviated by using either the Tiedtke or Kain–Fritsch schemes over the ocean instead. As few studies have applied and evaluated the Tiedtke and Kain–Fritsch schemes to the Korean region within the RegCM framework, and this study introduces the potential of these new CPSs compared with the more frequently selected Emanuel scheme, which is particularly beneficial to RegCM users.

Dietary copper supplementation reverses pressure overload-induced cardiac hypertrophy by copper replenishment in the heart. A copper-selective chelator, trientine (triethylenetetramine [TETA]), reverses left ventricular hypertrophy associated with diabetes also by copper replenishment in the heart. The present study was undertaken to address the critical issue how TETA delivers copper to the heart. Adult male Sprague-Dawley rats were subjected to transverse aortic constriction (TAC) to induce cardiac hypertrophy. Eight weeks after the TAC surgery, cardiac hypertrophy was developed and copper content in the heart was reduced. TETA was then administrated by gavage in two different dosages (21.9 or 87.6 mg/kg day) for six weeks. The results showed that in the lower dosage, TETA replenished copper contents in the heart, along with a decrease in the copper concentration in the blood and kidney, and an increase in the urine. In the higher dosage, TETA did not replenish copper contents in the heart, but markedly increased copper concentrations in the urine and decreased those in the blood and kidney. Neither lower nor higher TETA dosage altered copper concentrations in other organs. Corresponding to myocardial copper replenishment, the lower dose TETA suppresses cardiac hypertrophy, as judged by a reduction in the left ventricle wall thickness and a decrease in the heart size, and diminished cardiac fibrosis, as reflected by a decrease in collagen I content. TETA in the higher dose not only did not suppress cardiac hypertrophy, but also caused cardiac hypertrophy in sham-operated rats. TETA-mediated myocardial copper restoration is independent of copper transporter-1 or -2 but related to an energy-dependent transportation. This study demonstrates that low-dose TETA functions as a copper chaperone, selectively delivering copper to the copper-deprived heart through an active transportation; in higher doses, TETA simply retains its chelator function, removing copper from the body by urinary excretion. Impact statement Our study reveals that TETA, traditionally regarded as a copper chelator, in lower doses delivers copper selectively to the heart through a mechanism independent of copper transporter-1 or -2. Copper supplementation by a lower dose of TETA suppresses pressure overload-induced cardiac hypertrophy. Since ischemic heart disease and hypertrophic cardiomyopathy are accompanied by myocardial copper loss, this approach of using a lower dose of TETA to supplement copper to the heart would help treat the disease condition of patients with such cardiac events.

A versatile flow cytometric strategy is developed for the sensitive detection of plant microRNA (miRNA) by coupling the target-templated click nucleic acid ligation (CNAL) with on-bead terminal enzymatic DNA polymerization (TEP). Unlike ligase-catalyzed ligation reaction, the plant miRNA-templated enzyme-free CNAL between two single-stranded DNA (ssDNA) probes, respectively modified with Aza-dibenzocyclooctyne (Aza-DBCO) and N3, can not only simplify the operation, but also achieve a much higher ligation efficiency. More importantly, the undesirable nonspecific ligation between the Aza-DBCO- and N3-modified ssDNA, can be effectively eliminated by adding Tween-20, which allows the use of cycling CNAL (CCNAL) in a background-free manner. So each plant miRNA can template many rounds of CNAL reaction to produce numerous ligation products, forming efficient signal amplification. The ligated ssDNA can be anchored on the magnetic beads (MBs) with the 3′-OH termini exposed outside. Then terminal deoxynucleotidyl ...

This study evaluates the resolution dependency of scaling precipitation with temperature from the perspective of the added value of high-resolution (5 km) dynamical downscaling using various kinds of long-term climate change projections over South Korea. Three Coupled Model Intercomparison Project Phase 5 (global climate models (GCMs) with different climate sensitivities, and one pseudo global warming (PGW) experiment, are downscaled by Weather Research and Forecasting one-way double nested modeling system with convective parameterization for the reference (1976–2005) and future (2071–2100) periods under RCP8.5 scenario. A detailed comparison of the driving GCM/PGW, 20 km mother simulation, and 5 km nested simulation demonstrates improved representation of precipitation with increasing resolution not only in the spatial pattern and magnitude for both the mean and the extremes, but also in a more realistic representation of extreme precipitation’s sensitivities to temperature. According to the projected precipitation changes downscaled from both GCM ensemble and PGW, there will be intensified precipitation, particularly for the extremes, over South Korea under the warming, which is primarily contributed by convective precipitation increase that shows higher temperature sensitivity. This study also compares the extreme precipitation-temperature scaling relations within-epoch (apparent scaling) and between-epoch (climate scaling). It confirms that the magnitude and spatial pattern of the two scaling rates can be quite different, and the precipitation change over Korea under global warming is mainly controlled by thermodynamic factors.

Aqueous rechargeable Zn-ion batteries (AZIBs) have regarded as promising secondary chemical battery system because of the excellent safety, low cost and environmental friendliness. Nonetheless, it is still a great challenge to exploit suitable cathode materials for the insertion of zinc ions. The vanadium oxides-based materials are regarded as hopeful cathode materials of AZIBs because of their various coordination numbers and oxidation states. Especially, the hydrated, monovalent (divalent)-cations co-preinserted vanadium oxides show more excellent electrochemical performance because of the enlarged interlayer space. In this review, a comprehensive overview of the energy storage mechanisms and research development of various efficient ways to improve electrochemical performance for vanadium oxides-based compounds is presented. Finally, some insights into the future developments, challenges, and prospects of vanadium oxides-based compounds for AZIBs are proposed. This review illustrates electrochemical reaction mechanism of vanadium oxides-based compounds for AZIBs, and benefits the discovery of new AZIBs cathode materials, gives a novel viewpoint to shed light on the future innovation in vanadium oxides-based cathode materials, then facilitates the fast development and large-scale applications of AZIBs. The article will provide useful guidance for future design and construction of high-performance cathode materials for AZIBs.