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Allergic diseases such as asthma and rhinitis are common in many countries. Globally the most common allergen associated with symptoms is produced by house dust mites. Although the bed has often been cited as the main site of exposure to mite allergens, surprisingly this has not yet been directly established by measurement due to a lack of suitable methods. Here we report on the development of novel methods to determine the pattern of personal exposure to mite allergen bioaerosols over 24-hour periods and applied this in a small field study using 10 normal adults. Air was sampled using a miniature time-based air-sampler of in-house design located close to the breathing zone of the participants, co-located with a miniature time-lapse camera. Airborne particles, drawn into the sampler at 2L/min via a narrow slot, were impacted onto the peripheral surface of a disk mounted on the hour-hand of either a 12 or 24 hour clock motor. The impaction surface was either an electret cloth, or an adhesive film; both novel for these purposes. Following a review of the time-lapse images, disks were post-hoc cut into subsamples corresponding to eight predetermined categories of indoor or outdoor location, extracted and analysed for mite allergen Der p 1 by an amplified ELISA. Allergen was detected in 57.2% of the total of 353 subsamples collected during 20 days of sampling. Exposure patterns varied over time. Higher concentrations of airborne mite allergen were typically measured in samples collected from domestic locations in the day and evening. Indoor domestic Der p 1 exposures accounted for 59.5% of total exposure, whereas total in-bed-asleep exposure, which varied 80 fold between individuals, accounted overall for 9.85% of total exposure, suggesting beds are not often the main site of exposure. This study establishes the feasibility of novel methods for determining the time-geography of personal exposure to many bioaerosols and identifies new areas for future technical development and clinical applications.

Background: Carpets are large reservoirs of the dust mite allergen Der p 1. The effect of vacuum cleaning on the distribution of Der p 1 in carpets is poorly understood., Methods: Samples were cut from 7 used household carpets, all over 5 years of age. From each carpet, 10 samples were left untreated, 10 vacuumed with an upright vacuum cleaner, and 10 vacuumed with a canister vacuum cleaner. Each section was then cut into 3 horizontal layers: the top 2mm, the remainder of the carpet pile, and the carpet base. The mass of Der p 1 as a proportion of carpet volume was then determined., Results: The concentrations of Der p 1 in each depth layer varied considerably between the 7 untreated carpets. In the top layer, Der p 1 concentrations were (median; 25th-75th percentiles): 41.9; 28.3-92.6 pg/mm3. For the middle layer they were similar (38.1; 22.4-108.5 pg/mm3), and for the carpet base, higher (212.4; 98.8-456.2 pg/mm3). In most cases, cleaning using either type of vacuum cleaner resulted in no significant reduction in allergen concentration throughout all depth layers. A subset of carpets showed an apparent increase in Der p 1 concentration in one or more layers following vacuum cleaning. In all tests Der p 1 was collected in the vacuum cleaners' filter bags., Conclusions: The depth-distribution of Der p 1 differs widely amongst used carpets. Vacuum cleaning changes the distribution of Der p 1 within such carpets but does not necessarily result in a reduction in the overall content.

Seventeen pungent oleoresin principles of ginger (Zingiber officinale, Roscoe) and synthetic analogues were evaluated for inhibition of cyclooxygenase-2 (COX-2) enzyme activity in the intact cell. These compounds exhibited a concentration and structure dependent inhibition of the enzyme, with IC(50) values in the range of 1-25 microM. Ginger constituents, [8]-paradol and [8]-shogaol, as well as two synthetic analogues, 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decane and 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)dodecane, showed strong inhibitory effects on COX-2 enzyme activity. The SAR analysis of these phenolic compounds revealed three important structural features that affect COX-2 inhibition: (i) lipophilicity of the alkyl side chain, (ii) substitution pattern of hydroxy and carbonyl groups on the side chain, and (iii) substitution pattern of hydroxy and methoxy groups on the aromatic moiety., (Copyright 2001 Academic Press.)