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14. Effect of the gene silencing of phosphorus transporters on phosphorus absorption across primary cultured duodenal epithelial cell monolayers of chick embryos

Author

LI, Ting-ting, LU, Na, SHAO, Yu-xin, ZHANG, Li-yang, LU, Lin, LIU, Zong-ping, LUO, Xu-gang, and LIAO, Xiu-dong

Abstract

The aim of the study was to investigate whether phosphorus (P) transporters, type IIb sodium-dependent phosphate cotransporter (NaP-IIb) and inorganic phosphate transporter 2 (PiT2), were directly involved in P absorption across primary cultured duodenal epithelial cell monolayers of chick embryos. The siRNAs against NaP-IIb or PiT2 were designed, synthesized and transfected into primary cultured duodenal epithelial cells of chick embryos. Then, the inhibitory efficiency of siRNAs against NaP-IIb or PiT2 was analyzed, and the most efficacious siRNAs were selected to be used for subsequent P absorption experiments. Briefly, primary cultured duodenal epithelial cells of chick embryos were transfected with either NaP-IIb or PiT2 siRNAs and grown in confluent monolayers on transwell plates. The untransfected or transfected cell monolayers were then incubated in an uptake medium containing 0 or 0.25 mmol L–1of P as KH2PO4to measure the P absorption across duodenal epithelial cell monolayers. The results showed that among the siRNAs designed, si-1372 and si-890 were demonstrated to be the most effective in inhibiting the NaP-IIb and PiT2 expressions, respectively. Supplemental P increased (P=0.065) the protein abundance of PiT2 and enhanced (P<0.0001) P absorption in primary cultured duodenal epithelial cell of chick embryos. Furthermore, NaP-IIb silencing decreased (P=0.07) P absorption across duodenal epithelial cell monolayers, while PiT2 silencing had no effect (P=0.345). It is concluded that the NaP-IIb, but not PiT2, might be directly involved in the P absorption of chick duodenal epithelial cells.

Published
2022
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19. ROS-Mediated Cell Cycle Arrest and Apoptosis Induced by Zearalenone in Mouse Sertoli Cells via ER Stress and the ATP/AMPK Pathway

Author

Zheng, Wang-Long, primary, Wang, Bing-Jie, additional, Wang, Ling, additional, Shan, Yu-Ping, additional, Zou, Hui, additional, Song, Rui-Long, additional, Wang, Tao, additional, Gu, Jian-Hong, additional, Yuan, Yan, additional, Liu, Xue-Zhong, additional, Zhu, Guo-Qiang, additional, Bai, Jian-Fa, additional, Liu, Zong-Ping, additional, and Bian, Jian-Chun, additional

Published
2018
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23. [Untitled]

Author

Liu Zong-ping

Subjects
endocrine system, Kidney, medicine.medical_specialty, Veterinary medicine, General Veterinary, biology, Globulin, Serum albumin, Albumin, Camelus bactrianus, chemistry.chemical_element, Ovary, General Medicine, biology.organism_classification, medicine.anatomical_structure, Endocrinology, chemistry, Internal medicine, medicine, biology.protein, Bactrian camel, Selenium
Abstract

Reference values were established for some haematological and serum biochemical constituents in Bactrian camels (Camelus bactrianus) in China. The contents of seven trace elements in the blood, hair, liver, kidney, heart, spleen, lung, cerebrum, cerebellum, rib, ovary, pancreas and gluteal muscle of Bactrian camels were also measured. Some of these values are reported for the first time for Bactrian camels in China. Most haematological and serum biochemical values were similar to those of cattle, yaks, sheep and dromedary camels, but the mean serum albumin concentration and the albumim/globulin ratio were significantly higher than those in other ruminants and the mean thyroxine concentration was half that in dromedary camels. The liver contained the highest concentrations of copper, zinc, manganese and iron, and the renal cortex contained the highest concentration of selenium. The concentrations of selenium, cobalt, zinc, manganese and molybdenum in the tissues were within the reference ranges for other ruminants, but the mean iron and copper concentrations in the liver were significantly higher than those in other ruminants.

Published
2003

26. Corrigendum to “Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of sertoli cells” [Environ. Toxicol. Pharmacol. 42 (2016) 146–155]

Author

Zheng, WangLong, primary, Pan, ShunYe, additional, Wang, Guangguang, additional, Wang, Ya Jun, additional, Liu, Qing, additional, Gu, JianHong, additional, Yuan, Yan, additional, Liu, Xue Zhong, additional, Liu, Zong Ping, additional, and Bian, Jian Chun, additional

Published
2016
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35. Avian influenza A/H7N9 risk perception, information trust and adoption of protective behaviours among poultry farmers in Jiangsu Province, China.

Author

Bin Cui, Qiuyan Liao, Wendy Wing Tak Lam, Zong Ping Liu, Fielding, Richard, Cui, Bin, Liao, Qiuyan, Lam, Wendy Wing Tak, and Liu, Zong Ping

Subjects
AVIAN influenza A virus, HEALTH of farmers, POULTRY farms, MASS media, STRUCTURAL equation modeling, INFLUENZA prevention, AGE distribution, ANIMALS, INDUSTRIAL hygiene, INTENTION, MEDICINE information services, SENSORY perception, POULTRY, SELF-efficacy, SEX distribution, TRUST, OCCUPATIONAL hazards, ENVIRONMENTAL exposure, INFLUENZA A virus, RELATIVE medical risk, HEALTH information services
Abstract

Background: Poultry farmers are at high-risk from avian influenza A/H7N9 infection due to sustained occupational exposures to live poultry. This study examined factors associated with poultry farmers' adoption of personal protective behaviours (PPBs) based on Protection Motivation Theory (PMT).Methods: Totally, 297 poultry farmers in three cities of Jiangsu Province, China were interviewed during November 2013-January 2014. Data on PMT constructs, perceived trustworthiness of A/H7N9 information from mass media (formal sources), friends and family (informal sources), intention to adopt and actual adoption of PPBs and respondents' demographics were collected. Structural equation modeling (SEM) identified associations between demographic factors and PMT constructs associated with A/H7N9-oriented PPB intention. Moderated mediation analysis examined how demographics moderated the effects of information trust on PPB intention via risk perceptions of A/H7N9.Results: Respondents generally perceived low vulnerability to A/H7N9 infection. The SEM found that male respondents perceived lower severity of (β = -0.23), and lower vulnerability to (β = -0.15) A/H7N9 infection; age was positively associated with both perceived personal vulnerability to (β = 0.21) and perceived self-efficacy (β = 0.24) in controlling A/H7N9; education was positively associated with perceived response efficacy (β = 0.40). Furthermore, perceived vulnerability (β = 0.16), perceived self-efficacy (β = 0.21) and response efficacy (β = 0.67) were positively associated with intention to adopt PPBs against A/H7N9. More trust in informal information (TII) was only significantly associated with greater PPB intention through its positive association with perceived response efficacy. Age significantly moderated the associations of TII with perceived Self-efficacy and perceived response efficacy, with younger farmers who had greater TII perceiving lower self-efficacy but higher response efficacy.Conclusion: Poultry farmers perceive A/H7N9 as a personally-irrelevant risk. Interventions designed to enhance perceived response efficacy, particularly among lower educated respondents may effectively motivate adoption of PPBs. Informal information may be an important resource for enhancing response efficacy. [ABSTRACT FROM AUTHOR]

Published
2017
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36. Studies on rickets and osteomalacia in Bactrian camels (Camelus bactrianus)

Author

Liu Zong-ping

Subjects
medicine.medical_specialty, China, Camelus, chemistry.chemical_element, Camelus bactrianus, Rickets, Bone meal, Animal science, Internal medicine, medicine, Animals, Bactrian camel, Phosphorus deficiency, Osteomalacia, General Veterinary, biology, business.industry, Phosphorus, biology.organism_classification, medicine.disease, Animal Feed, Endocrinology, chemistry, Liver, Alkaline phosphatase, Animal Science and Zoology, business, Blood Chemical Analysis, Hair
Abstract

Epidemiological studies have indicated incidences of 32.9% and 27.8% for rickets and osteomalacia, respectively, in Bactrian camels (Camelus bactrianus), but there is an increased incidence under drought conditions, sometimes reaching 75%. We have found that concentrations of phosphorus and copper in forage and soil samples in a drought affected area were significantly lower than in a control area or normal reference values (P0.01) ; the mean Ca:P ratio in the forages was 50:1. The phosphorus content of blood and hair from affected camels was significantly less than that in controls (P0.01) and concentrations of copper in the liver and kidney were significantly lower in affected camels than control animals (P0.01); the concentrations of triiodothyronine (T(3)), thyroxine (T(4)) and parathyroid hormone (PTH) in the serum from affected animals were significantly higher than those from healthy controls (P0.01); serum inorganic phosphorus and ceruloplasmin levels were lower than those in the controls (P0.01 or P0.05); the concentrations of serum alpha-globulin and beta-globulin were significantly higher in the affected camels than in the healthy controls (P0.01). The pathological changes seen in camels affected with rickets included porous, brittle, light, osteoporotic bones that were susceptible to fractures and had less resistance to cutting and sawing. Wrist joints were enlarged with an apparent bowing of the long bones in forelimb and with typical broadening of the epiphyses. In adult female camels, many enlarged scars were often seen in ribs indicating earlier fractures. The disease could be cured with supplementary bone meal, phosphate or mineral mixtures and in field investigations clinical signs disappeared within 15 days. Over the same period, the concentrations of phosphorus and alkaline phosphatase in blood returned to normal. The disease may be effectively prevented by use of mineral blocks (block salt licks) or dosing orally with copper, selenium and cobalt soluble glass boluses. We conclude that rickets and osteomalacia are mainly caused by phosphorus and copper deficiencies in the pasture.

Published
2004

44. Cadmium Activates Reactive Oxygen Species-dependent AKT/mTOR and Mitochondrial Apoptotic Pathways in Neuronal Cells.

Author

YUAN, Yan, WANG, Yi, HU, Fei Fei, JIANG, Chen Yang, ZHANG, Ya Jing, YANG, Jin Long, ZHAO, Shi Wen, GU, Jian Hong, LIU, Xue Zhong, BIAN, Jian Chun, and LIU, Zong Ping

Subjects
CADMIUM analysis, REACTIVE oxygen species, MTOR protein, PROTEIN kinase B, APOPTOSIS, NEURON analysis
Abstract

Objective To examine the role of Cd-induced reactive oxygen species (ROS) generation in the apoptosis of neuronal cells. Methods Neuronal cells (primary rat cerebral cortical neurons and PC12 cells) were incubated with or without Cd post-pretreatment with rapamycin (Rap) or N-acetyl- l -cysteine (NAC). Cell viability was determined by MTT assay, apoptosis was examined using flow cytometry and fluorescence microscopy, and the activation of phosphoinositide 3′-kinase/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) and mitochondrial apoptotic pathways were measured by western blotting or immunofluorescence assays. Results Cd-induced activation of Akt/mTOR signaling, including Akt, mTOR, p70 S6 kinase (p70 S6K), and eukaryotic initiation factor 4E binding protein 1 (4E-BP1). Rap, an mTOR inhibitor and NAC, a ROS scavenger, blocked Cd-induced activation of Akt/mTOR signaling and apoptosis of neuronal cells. Furthermore, NAC blocked the decrease of B-cell lymphoma 2/Bcl-2 associated X protein (Bcl-2/Bax) ratio, release of cytochrome c, cleavage of caspase-3 and poly(ADP-ribose) polymerase (PARP), and nuclear translocation of apoptosis-inducing factor (AIF) and endonuclease G (Endo G). Conclusion Cd-induced ROS generation activates Akt/mTOR and mitochondrial pathways, leading to apoptosis of neuronal cells. Our findings suggest that mTOR inhibitors or antioxidants have potential for preventing Cd-induced neurodegenerative diseases. [ABSTRACT FROM AUTHOR]

Published
2016
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