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1. Altered Liver Enzyme Markers in Patients with Asymptomatic, and Mild Omicron Infection: A Retrospective Study

Author

Cao X, Xie YL, Yi JY, Liu ZL, Han M, Duan JH, Gao Q, Mu H, and Zhou CL

Subjects
omicron variant, ‘abnormal liver enzyme markers’, male, inflammatory markers., Pathology, RB1-214, Therapeutics. Pharmacology, RM1-950
Abstract

Xi Cao,1 Yong-Li Xie,2,3 Jian-Ying Yi,1 Zhi-Li Liu,4 Meng Han,1 Ji-hui Duan,1 Qiang Gao,1 Hong Mu,1 Chun-lei Zhou1 1Department of Clinical Laboratory, Tianjin First Central Hospital, Tianjin, People’s Republic of China; 2Department of Clinical Laboratory, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin, People’s Republic of China; 3Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin, People’s Republic of China; 4Department of Clinical Laboratory, the Third Central Hospital, Tianjin, People’s Republic of ChinaCorrespondence: Hong Mu; Chun-lei Zhou, Department of Clinical Laboratory, Tianjin First Central Hospital, Tianjin, People’s Republic of China, Email mutjyzxyy@163.com; tj_zcl@hotmail.comPurpose: The emergence of the SARS-CoV-2 Omicron variant has posed a significant global public health challenge. Elucidating the laboratory profiles of individuals infected with this variant is crucial for assessing organ damage. This study aimed to investigate the variations in liver function tests and their correlation with demographic characteristics and inflammatory markers in patients with early Omicron variant infections.Patients and Methods: A retrospective cohort study was conducted on 1133 mild or asymptomatic COVID-19 cases at Tianjin First Central Hospital. Data on age, gender, body mass index (BMI), and serum markers were collected and analyzed. Statistical analyses were performed using SPSS software, version 24.0.Results: Abnormal liver function parameters, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and total bilirubin (TBIL), were observed in 314 (27.71%) patients. “Hepatocellular type” was identified in 56 (4.94%) patients, “cholestatic type” in 185 (16.33%) patients, and “mixed type” in 73 (6.44%) patients. In the mixed group, we observed a pronounced elevation in the levels of ALT, AST, and GGT. Moreover, the hepatocellular group exhibited a statistically significant increase in AST and ALT concentrations relative to both the normal and cholestatic groups. Notably, the cholestatic group demonstrated a substantial increment in ALP levels. Males had a significantly higher prevalence of “abnormal liver enzyme markers” compared to females. Patients with “abnormal liver enzyme markers” exhibited significantly decreased immunoglobulin G (IgG) levels and elevated levels of inflammatory markers, including procalcitonin (PCT), interleukin-6 (IL6), as well as C-reactive protein (CRP) compared to normal group. Logistic regression analysis revealed that male gender and PCT levels were significantly associated with the risk of abnormal liver enzyme markers. Patients in hepatocellular group were likely accompanied with high CRP levels, whereas those in the cholestatic type were associated with high IL6 levels.Conclusion: Early Omicron infection might cause liver stress response. Elevated liver enzyme marker levels were correlated with age, gender, inflammatory factors, and IgG.Keywords: Omicron variant, “abnormal liver enzyme markers”, male, inflammatory markers

Published
2024

2. Identification of a Novel Pyroptosis-Related Gene Signature Indicative of Disease Prognosis and Treatment Response in Skin Cutaneous Melanoma

Author

Li AA, Zhang Y, Tong WL, Chen JW, Huang SH, Liu JM, and Liu ZL

Subjects
skin cutaneous melanoma, pyroptosis, prognosis, signature, risk model, Medicine (General), R5-920
Abstract

An-An Li,1,2 Yu Zhang,1,2 Wei-Lai Tong,1,2 Jiang-Wei Chen,1 Shan-Hu Huang,1 Jia-Ming Liu,1 Zhi-Li Liu1,2 1Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 2Medical Innovation Center, The First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of ChinaCorrespondence: Zhi-Li Liu; Jia-Ming Liu, Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Street, Donghu District, Nanchang, 330006, People’s Republic of China, Tel/Fax +86-791-88693201, Email liuzhiliyfy@163.com; zgm7977@ncu.edu.cn; liujiamingdr@hotmail.comPurpose: Pyroptosis plays an important role in the occurrence and progression of many tumors; however, the specific mechanisms involved remain unknown. Here, we construct a pyroptosis-related gene signature that can be used to predict survival prognosis of skin cutaneous melanoma (SKCM) and provide guidance for clinical treatment.Methods: By integrating data from the two databases from the GTEx and TCGA, differentially expressed genes (DEGs) from normal tissues and skin cutaneous tumor tissues were identified. The main signaling pathways and function enrichment of these differential genes were determined. Univariate and multivariate COX regression analysis, and risk score analysis were used to construct a signature to assess its predictive value for overall survival. The mRNA expression of these five genes in melanoma cells was determined by quantitative polymerase chain reaction (qPCR). The pRRophetic algorithm was used to estimate the half-maximal inhibitory concentration (IC50) of chemotherapy drugs in SKCM patients. The expression of multiple immune checkpoint genes also was evaluated.Results: Sixteen DEGs associated with pyroptosis in SKCM and normal skin tissues were identified. Of these, 12 pyroptosis-related DEGs were associated with the prognosis of SKCM. A five-gene signature (GSDMA, GSDMC, IL-18, NLRP6, and AIM2) model was constructed. Patients were divided into high-risk and low-risk groups using the risk scores. Of these, the high-risk group had a worse survival prognosis. There are significant differences in the predicted sensitivity of the high-risk and low-risk groups to chemotherapeutic drugs. In addition, compared with the high-risk group, the low-risk group showed higher expression of PD-1, PDL-1, CTLA-4, LAG-3, and VSIR.Conclusion: In this study, we constructed a novel prognostic pyroptosis-related gene-signature for SKCM. These genes showed good predictive value for patient prognosis and could provide guidance for better treatment of SKCM patients.Keywords: skin cutaneous melanoma, pyroptosis, prognosis, signature, risk model

Published
2022

3. Application of Machine Learning Techniques to Predict Bone Metastasis in Patients with Prostate Cancer

Author

Liu WC, Li MX, Qian WX, Luo ZW, Liao WJ, Liu ZL, and Liu JM

Subjects
prostate cancer, bone metastasis, machine learning, prediction model, seer, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Wen-Cai Liu,1,2 Ming-Xuan Li,2 Wen-Xing Qian,3 Zhi-Wen Luo,1,4 Wei-Jie Liao,1,4 Zhi-Li Liu,1,4 Jia-Ming Liu1,4 1Department of Orthopaedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People’s Republic of China; 2The First Clinical Medical College of Nanchang University, Nanchang, 330006, People’s Republic of China; 3School of Computer and Information Technology, Beijing Jiaotong University, Beijing, 100044, People’s Republic of China; 4Institute of Spine and Spinal Cord, Nanchang University, Nanchang, 330006, People’s Republic of ChinaCorrespondence: Jia-Ming LiuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Street, Donghu District, Nanchang, Jiangxi Province, People’s Republic of ChinaTel/Fax +86-791-86319815Email liujiamingdr@hotmail.comObjective: This study aimed to develop and validate a machine learning model for predicting bone metastases (BM) in prostate cancer (PCa) patients.Methods: Demographic and clinicopathologic variables of PCa patients in the Surveillance, Epidemiology and End Results (SEER) database from 2010 to 2017 were retrospectively analyzed. We used six different machine learning algorithms, including Decision tree (DT), Random forest (RF), Multilayer Perceptron (MLP), Logistic regression (LR), Naive Bayes classifiers (NBC), and eXtreme gradient boosting (XGB), to build prediction models. External validation using data from 644 PCa patients of the First Affiliated Hospital of Nanchang University from 2010 to 2016. The performance of the models was evaluated using the area under receiver operating characteristic curve (AUC), accuracy score, sensitivity (recall rate) and specificity. A web predictor was developed based on the best performance model.Results: A total of 207,137 PCa patients from SEER were included in this study. Of whom, 6725 (3.25%) developed BM. Gleason score, Prostate-specific antigen (PSA) value, T, N stage and age were found to be the risk factors of BM. The XGB model offered the best predictive performance among these 6 models (AUC: 0.962, accuracy: 0.884, sensitivity (recall rate): 0.906, and specificity: 0.879). An XGB model-based web predictor was developed to predict BM in PCa patients.Conclusion: This study developed a machine learning model and a web predictor for predicting the risk of BM in PCa patients, which may help physicians make personalized clinical decisions and treatment strategy for patients.Keywords: prostate cancer, bone metastasis, machine learning, prediction model, SEER

Published
2021

4. Effect of Platelet-Activating Factor on Barrier Function of ARPE-19 Cells

Author

Zhang F, Liu L, Zhang H, and Liu ZL

Subjects
platelet-activating factor, arpe-19 cells, barrier function, vascular endothelial growth factor, Therapeutics. Pharmacology, RM1-950
Abstract

Fan Zhang,1 Lei Liu,2 Han Zhang,2 Zhe-Li Liu2 1Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang, Liaoning, People’s Republic of China; 2Department of Ophthalmology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of ChinaCorrespondence: Han ZhangDepartment of Ophthalmology, The First Affiliated Hospital of China Medical University, 155 Nanjing North St, Heping District, Shenyang 110001, Liaoning, People’s Republic of ChinaTel +86-24-83283590Fax +86-24-83282997Email zhanghan0614@139.comAim: To examine the effects of platelet-activating factor (PAF) on the barrier functions of cultured retinal pigment epithelial (RPE) cells.Methods: A human RPE cell line (ARPE-19) was cultured on microporous filter supports and treated with PAF and WEB 2086, a specific PAF-receptor (PAF-R) antagonist. The permeability of the RPE monolayer was measured using transepithelial electrical resistance (TER) and sodium fluorescein flux. The expression of the tight junction protein zonula occludens (ZO)-1 and the adherens junction protein N-cadherin was assessed using immunohistochemistry and Western blotting. We also measured the vascular endothelial growth factor (VEGF) concentrations in PAF-treated cultures and re-measured RPE monolayer permeability in the presence of VEGF-neutralizing antibodies.Results: PAF significantly decreased the TER and enhanced the sodium fluorescein flux of the RPE monolayer and downregulated the expression of ZO-1 and N-cadherin. These effects were abolished by WEB 2086-mediated blockage of the PAF-R. PAF stimulation increased VEGF expression in RPE cells, and the antibody-mediated neutralization of VEGF caused a partial recovery of the barrier properties.Conclusion: The barrier functions of ARPE-19 cells were altered by PAF, and these effects were partly mediated by an upregulation of VEGF expression in these cells. Our results contribute to the growing body of evidence supporting the role of PAF in choroidal neovascularization. Our findings suggest that PAF is a novel target in the development of therapies for increased permeability of the RPE monolayer.Keywords: platelet-activating factor, ARPE-19 cells, barrier function, vascular endothelial growth factor

Published
2020

5. A review on nanosystems as an effective approach against infections of Staphylococcus aureus

Author

Zhou KX, Li C, Chen DM, Pan YH, Tao YF, Qu W, Liu ZL, Wang XF, and Xie SY

Subjects
Staphylococcus aureus, Infection mechanism, Resistance, Antibiotics, Nanoparticles, Medicine (General), R5-920
Abstract

Kaixiang Zhou,1 Chao Li,1 Dongmei Chen,2 Yuanhu Pan,1 Yanfei Tao,2 Wei Qu,2 Zhenli Liu,2 Xiaofang Wang,3 Shuyu Xie1 1MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei, China; 2National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Wuhan, Hubei, China; 3Animal Husbandry and Veterinary Institute of Hebei Province, Baoding, Hebei, China Abstract: Staphylococcus aureus (S. aureus) is an important zoonotic bacteria and hazardous for the health of human beings and livestock globally. The characteristics like biofilm forming, facultative intracellular survival, and growing resistance of S. aureus pose a great challenge to its use in therapy. Nanoparticles are considered as a promising way to overcome the infections’ therapeutic problems caused by S. aureus. In this paper, the present progress and challenges of nanoparticles in the treatment of S. aureus infection are focused on stepwise. First, the survival and infection mechanism of S. aureus are analyzed. Second, the treatment challenges posed by S. aureus are provided, which is followed by the third step including the advantages of nanoparticles in improving the penetration and accumulation ability of their payload antibiotics into cell, inhibiting S. aureus biofilm formation, and enhancing the antibacterial activity against resistant isolates. Finally, the challenges and future perspective of nanoparticles for S. aureus infection therapy are introduced. This review will help the readers to realize that the nanosystems can effectively fight against the S. aureus infection by inhibiting biofilm formation, enhancing intracellular delivery, and improving activity against methicillin-resistant S. aureus and small colony variant phenotypes as well as aim to help researchers looking for more efficient nanosystems to combat the S. aureus infections. Keywords: Staphylococcus aureus, infection mechanism, resistance, antibiotics, nanoparticles

Published
2018

6. Let-7a inhibits osteosarcoma cell growth and lung metastasis by targeting Aurora-B

Author

Yu JJ, Pi WS, Cao Y, Peng AF, Cao ZY, Liu JM, Huang SH, Liu ZL, and Zhang W

Subjects
let-7a, Aurora-B, Osteosarcoma, malignant phenotype, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Jing-Jing Yu,1,* Wen-Sen Pi,2,* Yuan Cao,3 Ai-Fen Peng,4 Zhi-Yuan Cao,2 Jia-Ming Liu,2 Shan-Hu Huang,2 Zhi-Li Liu,2 Wei Zhang1 1Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang 330006, People’s Republic of China; 2Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, People’s Republic of China; 3Department of Medical Imaging, The First Clinical Medical School of Nanchang University, Nanchang 330006, People’s Republic of China; 4College of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang 330001, People’s Republic of China *These authors contributed equally to this work Purpose: Accumulating studies showed that the expression of microRNAs (miRNAs) was dysregulated in osteosarcoma (OS). In this study, we sought to investigate the effect of let-7a on OS progression and its potential molecular mechanism.Patients and methods: Quantitative real-time PCR (qRT-PCR) was performed to evaluate the expression level of let-7a and Aurora-B (AURKB) in OS tissues and cells. The OS cells were treated with let-7a mimic, let7a inhibitor, negative mimic and Lv-AURKB combined with let-7a. The ability of cell proliferation, migration and invasion was measured using Cell Counting Kit-8 (CCK-8) and wound-healing and transwell invasion assays. The protein of AURKB, NF-κβp65, MMP2 and MMP9 was measured by Western blot analysis. Xenograft model was performed to investigate the effects of let-7a on tumor growth and metastasis. The lung metastasis was measured by counting the metastatic node using H&E staining.Results: Let-7a expression was significantly underexpressed in OS cell lines and tissues compared with human osteoblast cell lines, hFOB1.19, and adjacent normal bone tissues. Exogenous let-7a inhibited the viability, migratory and invasive ability of OS cells in vitro. In addition, the overexpression of AURKB in OS cells could partly rescue let-7a-mediated tumor inhibition. Also, the overexpression of let-7a inhibited OS cell growth and lung metastasis in vivo. Furthermore, the results showed that let-7a could decrease the expression of NF-κβp65, MMP2 and MMP9 proteins by targeting AURKB in OS cells.Conclusion: Let-7a inhibits the malignant phenotype of OS cells by targeting AURKB at least partially. Targeting let-7a and AURKB/NF-κβ may be a novel therapeutic strategy for the treatment of OS. Keywords: let-7a, Aurora-B, osteosarcoma, malignant phenotype

Published
2018

7. Expressions and prognostic values of the E2F transcription factors in human breast carcinoma

Author

Liu ZL, Bi XW, Liu PP, Lei DX, Wang Y, Li ZM, Jiang WQ, and Xia Y

Subjects
E2Fs, breast carcinoma, expressions, prognostic values, Kaplan-Meier plot, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Ze-Long Liu,* Xi-Wen Bi,* Pan-Pan Liu,* De-Xin Lei, Yu Wang, Zhi-Ming Li, Wen-Qi Jiang, Yi Xia Department of Medical Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Centre of Cancer Medicine, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: E2F transcription factors (E2Fs) are a family of transcription factors involved in cell proliferation, differentiation, and apoptosis. Their important roles in the development and metastasis of breast carcinoma (BC) have been discovered by previous in vitro and in vivo studies. Yet, expressions and distinct prognostic values of these eight E2Fs in human BC remain unclear in many respects. In this study, we aimed to reveal their roles in BC through analyzing the transcription and survival data of the E2Fs in BC patients from four online databases including ONCOMINE, Breast Cancer Gene-Expression Miner v4.1, cBioPortal for Cancer Genomics, and Kaplan–Meier Plotter. We found the overexpression of E2Fs in BC tissues compared with normal breast tissues, except for E2F4. Higher expression levels of E2Fs, except for E2F4 and E2F6, were associated with higher levels of Scarff–Bloom–Richardson grade of BC. Alterations of E2Fs were found to be significantly correlated with poorer overall survival of BC patients. Through plotting the survival curve in the Kaplan–Meier Plotter, it was found that higher mRNA levels of E2F1, E2F3, E2F7, and E2F8 were associated with poorer relapse-free survival in all BC patients, indicating that they are potential targets for individualized treatments of BC patients. Conversely, higher mRNA expression level of E2F4 predicted better RFS in BC patients, suggesting E2F4 as a new biomarker for BC prognosis. Considering currently available limited evidence, further studies need to be performed to investigate the roles of E2Fs in BC. Keywords: E2Fs, breast carcinoma, expressions, prognostic values, Kaplan–Meier plot

Published
2018

8. The effect of curcumin and its nanoformulation on adjuvant-induced arthritis in rats

Author

Zheng ZL, Sun YH, Liu ZL, Zhang MQ, Li CQ, and Cai H

Subjects
Therapeutics. Pharmacology, RM1-950
Abstract

Zhaoling Zheng,1,* YanHua Sun,2,* Ziliang Liu,1 Mingqin Zhang,1 Chunqing Li,1 Hui Cai3 1Department of Traditional Chinese Medicine, Dongying People’s Hospital, Dongying, 2Shandong Provincial Key Laboratory of Microparticles Drug Delivery Technology, Jinan, 3Department of Integrated Traditional Chinese Medicine and Western Medicine, Nanjing Jinling Hospital, Nanjing, People’s Republic of China *These authors contributed equally to this work Background: Rheumatoid arthritis (RA), induced by the prolonged inappropriate inflammatory responses, is one of the most prevalent of all chronic inflammatory joint diseases. Curcumin (CM), a yellow hydrophobic polyphenol derived from the herb turmeric, has various pharmacological activities against many chronic diseases and acts by inhibiting cell proliferation and metastasis and downregulating various factors, including nuclear factor kappa B, interleukin-1β and TNF-α. Given the pathogenesis of RA, we hypothesized that the drug also has antiarthritic effects. The aims of the present study included the following: 1) examining the therapeutic effect of CM administered via intravenous (iv) injection on RA and 2) formulating the drug into oil–water nanoemulsions (Ns) to overcome the low oral bioavailability of CM and achieve oral delivery of the drug.Methods: The effect of CM administered through iv injection on adjuvant-induced arthritis in rats was studied in terms of paw swelling, weight indices of the thymus and spleen, and pathological changes in nuclear factor kappa B expression and inflammatory cytokines. Methotrexate was used as a positive control. The CM-Ns were prepared using a high-pressure homogenizing method and characterized with respect to the particle size and morphology. The stability of the CM-Ns in simulated gastrointestinal (GI) fluids and in vitro release were also investigated. A pharmacokinetic study of the CM-Ns and suspensions in which the plasma levels were determined using an high performance liquid chromatography method and the pharmacokinetic parameters were calculated based on a statistical moment theory was also performed in rats.Results: CM administered via iv injection had a therapeutic effect on RA similar to methotrexate. CM-Ns with a diameter of approximately 150 nm were successfully prepared, and the drug was well encapsulated into the Ns without degradation in simulated GI conditions. The area under the curve (AUC) and Cmax for the CM-Ns were more than threefold greater than those for the suspensions; moreover, similar decreases in the levels of TNF-α and interleukin-1β in both synovial fluid and blood serum were obtained from oral administration of CM-Ns and iv injection.Conclusion: CM was an effective antiarthritic agent, and the present N formulation appeared to be a promising system that allowed RA therapy with CM to be converted from iv to oral administration. Keywords: curcumin, nanoemulsions, inflammatory cytokines, adjuvant-induced arthritis, drug release, oral bioavailability

Published
2015

9. Intrinsically patterned two-dimensional materials for selective adsorption of molecules and nanoclusters

Author

Lin, X, Lu, JC, Shao, Y, Zhang, YY, Wu, X, Pan, JB, Gao, L, Zhu, SY, Qian, K, Zhang, YF, Bao, DL, Li, LF, Wang, YQ, Liu, ZL, Sun, JT, Lei, T, Liu, C, Wang, JO, Ibrahim, K, Leonard, DN, Zhou, W, Guo, HM, Wang, YL, Du, SX, Pantelides, ST, and Gao, H-J

Subjects
Condensed Matter - Materials Science
Abstract

Two-dimensional (2D) materials have been studied extensively as monolayers 1-5, vertical or lateral heterostructures 6-8. To achieve functionalization, monolayers are often patterned using soft lithography and selectively decorated with molecules 9,10. Here we demonstrate the growth of a family of 2D materials that are intrinsically patterned. We demonstrate that a monolayer of PtSe2 can be grown on a Pt substrate in the form of a triangular pattern of alternating 1T and 1H phases. Moreover, we show that, in a monolayer of CuSe grown on a Cu substrate, strain relaxation leads to periodic patterns of triangular nanopores with uniform size. Adsorption of different species at preferred pattern sites is also achieved, demonstrating that these materials can serve as templates for selective self-assembly of molecules or nanoclusters, as well as for the functionalization of the same substrate with two different species.

Published
2020
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12. Cytochrome C Oxidase Assembly Factor 1 Homolog Predicts Poor Prognosis and Promotes Cell Proliferation in Colorectal Cancer by Regulating PI3K/AKT Signaling

Author

Xue Y, Li PD, Tang XM, Yan ZH, Xia SS, Tian HP, Liu ZL, Zhou T, Tang XG, and Zhang GJ

Subjects
coa1, proliferation, apoptosis, colorectal cancer, pi3k/akt signaling, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Abstract

Yuan Xue,1,2,* Pei-Dong Li,1,2,* Xue-Mei Tang,3,* Zai-Hua Yan,1,2 Shu-Sen Xia,1 Hong-Peng Tian,1 Zuo-Liang Liu,1 Tong Zhou,1 Xue-Gui Tang,4 Guang-Jun Zhang1,2 1The Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China; 2Institute of Hepatobiliary, Pancreatic and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China; 3Department of Ultrasound, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China; 4Anorectal Department of Integrated Traditional Chinese and Western Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xue-Gui TangAnorectal Department of Integrated Traditional Chinese and Western Medicine, The Affiliated Hospital of North Sichuan Medical College, 63 Wenhua Road, Nanchong 637000, Sichuan, People’s Republic of ChinaTel +86 817 2262419Email txg668nc@sohu.comGuang-Jun ZhangThe Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, 63 Wenhua Road, Nanchong 637000, Sichuan, People’s Republic of ChinaTel +86 817 2262419Email zhanggj1977@126.comPurpose: Colorectal cancer (CRC) is one of the most common malignancies in the world. The prognosis of advanced CRC is still poor. The purpose of this study was to identify a gene expression profile associated with CRC that may contribute to the early diagnosis of CRC and improve patient prognosis.Patients and Methods: Five pairs of CRC tissues and paracancerous tissues were used to identify causative genes using microarray assays. The prognostic value of Cytochrome C Oxidase Assembly Factor 1 Homolog (COA1) in CRC was assessed in 90 CRC patients. Loss-of-function assays, cell proliferation assays using Celigo and MTT, colony formation assays, a subcutaneous xenograft mouse model, and apoptosis assays were used to define the effects of downregulation of COA1 in CRC cells in vitro and in vivo. The underlying molecular mechanisms of COA1 in CRC were also investigated.Results: The causative gene COA1 was identified through microarray analysis. COA1 expression in CRC was notably associated with pathologic differentiation, tumor size, and tumor depth. COA1 expression may act as an independent prognostic factor for overall survival of CRC. Knockdown of COA1 inhibited the proliferation of CRC cells in vitro and the tumorigenicity of CRC cells in vivo. Decreased COA1 expression induced apoptosis of CRC cells. Based on the microarray assay results comparing HCT116 cells transfected with lentivirus encoding anti-COA1 shRNA or negative control shRNA, ingenuity pathway analysis (IPA) revealed that the PI3K/AKT signaling pathway was significantly enriched. Moreover, CCND1, mTOR, AKT1, and MDM2 were identified as the downstream genes of COA1.Conclusion: These findings demonstrate that COA1 promotes CRC cell proliferation and inhibits apoptosis by regulating the PI3K/AKT signaling pathway. Our results implicate COA1 as a potential oncogene involved in tumor growth and progression of CRC.Keywords: colorectal cancer, COA1, proliferation, apoptosis, PI3K/AKT signaling

Published
2020

13. Silicosis-associated Severe Pneumonia Treated by Veno-veno-venous Extracorporeal Membrane Oxygenation: a Case Report

Author

Liu Zl, Xiong T, Zhao Sp, and Su Xf

Subjects
surgical procedures, operative, genetic structures, nervous system, Silicosis, business.industry, Anesthesia, medicine.medical_treatment, medicine, Extracorporeal membrane oxygenation, Pneumonia treated, medicine.disease, business
Abstract

Background: Extracorporeal membrane oxygenation (ECMO) is an extracorporeal life support system (ECLS) for patients with severe cardiopulmonary failure, there are two basic patterns of veno-arterial (V-A) and veno-venous (V-V). Case presentation: This paper mainly introduced a death case of modified ECMO Patterns which is veno-veno-venous ECMO in the treatment of silicosis with severe pneumonia and analyzed the cause of death after treatment with VV-V ECMO. Conclusions: Although VV-V ECMO is recommended for patients with extremely poor oxygenation conditions, it still be difficult to improve the prognosis of patients with severe lung structural damage, and still has a long way to go.

Published
2021

15. Clinical implication of long noncoding RNA 91H expression profile in osteosarcoma patients

Author

Xia WK, Lin QF, Shen D, Liu ZL, Su J, and Mao WD

Subjects
long non-coding RNA, 91H, osteosarcoma, overall survival, biomarker, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Abstract

Wen-Kai Xia,1 Qing-Feng Lin,2 Dong Shen,2 Zhi-Li Liu,2 Jun Su,2 Wei-Dong Mao2 1Department of Nephrology, 2Department of Oncology, The Affiliated Jiangyin Hospital, School of Medicine, Southeast University, Jiangyin, Jiangsu, People’s Republic of China Abstract: Long noncoding RNAs have been documented as having widespread roles in carcinogenesis and cancer progression. However, roles of long noncoding RNAs in osteosarcoma remain unclear. This study is to investigate the clinical relevance and biological functions of long noncoding RNA 91H in osteosarcoma. Herein, we confirmed that 91H expression was notably increased in osteosarcoma patients and cell lines compared to healthy controls and normal human bone cell lines. High expression of 91H was significantly correlated with advanced clinical stage, chemotherapy after surgery, and tumor size >5 cm. Furthermore, 91H was an independent prognostic factor for overall survival in osteosarcoma patients after treatments. Additionally, the knockdown of 91H expression inhibited osteosarcoma cells’ proliferation and promoted their apoptosis in vitro. In summary, these findings indicate that 91H may be a novel biomarker for risk prognostication and also provide a clue to the molecular etiology of osteosarcoma. Keywords: long noncoding RNA, 91H, osteosarcoma, survival

Published
2016

17. Computation on dislocation-based crystal plasticity at micron-nano scales

Author

Zhuang, Z, Liu, ZL, and Cui, YN

Subjects
Condensed Matter::Materials Science, Engineering, Civil, Engineering, Aerospace
Abstract

Plastic flow in crystal at micron-nano scales involves many new interesting issues. Some results are obtained for uniaxial compression experiments conducted on FCC single crystal micro-pillars, e.g. size effect and strain burst, etc.. In these experiments, the surfaces are transmissible and loading gradients are absent. Therefore, the strain gradient theory could not well explain these new mechanical behaviors. This in turn has led to several hypotheses based on intuitive insights, classical theory and dislocation plasticity in order to study the size effect at submicron scale. In the model proposed, mobile dislocations may escape from the free surface leading to the state of dislocation starved whereby an increase in the applied stress is necessary to nucleate or activate new dislocation sources. By performing in-situ TEM, the dislocation motion affected the material properties is observed. However, the atypical plastic behavior at submicron scales cannot be effectively investigated by either traditional crystal plastic theory or large-scale molecule dynamics simulation. Accordingly, the discrete dislocation dynamics (DDD) coupling with finite element method (FEM), so a discrete-continuous crystal plastic model (DCM) is developed. Three kinds of plastic deformation mechanisms for the single crystal pillar at submicron scale are investigated. (1) Single arm dislocation source (SAS) controlled plastic flow. It is found that strain hardening is virtually absent due to continuous operation of stable SAS and weak dislocation interactions. When the dislocation density finally reaches stable value, a ratio between the stable SAS length and pillar diameter obeys a constant value. A theoretical model is developed to predict DDD simulation results and experimental data. (2) Confined plasticity in coated micropillars. Based on the simulation results and stochastic distribution of SAS, a theoretical model is established to predict the upper and lower bounds of stress-strain curve in the coated micropillars. (3) Dislocation starvation under low amplitude cyclic loading. This work argued that the dislocation junctions can be gradually destroyed during cyclic deformation, even when the cyclic peak stress is much lower than that required to break them under monotonic deformation. The cumulative irreversible slip is found to be the key factor of leading to junction destruction and promoting dislocation starvation under low amplitude cyclic loadings. Based on this mechanism, a proposed theoretical model successfully reproduces dislocation annihilation behavior observed experimentally for small pillar and dislocation accumulation behavior for large pillar. The predicted critical conditions of dislocation starvation agree well with the experimental data.

Published
2016

18. Gas Chromaotography-Mass Spectrometry Analysis of Insecticidal Essential Oil Derived from Chinese Ainsliaea fragrans Champ ex Benth (Compositae)

Author

Zhao, MP, Liu, XC, Liu, QZ, and Liu, ZL

Subjects
Ainsliaea fragrans, Sitophilus zeamais, Contact toxicity, Elemicine, Essential oil, Grain storage
Abstract

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Ainsliaea fragrans against maize weevils (Sitophilus zeamais).Methods: The essential oil of A. fragrans aerial parts was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). Contact toxicity of the essential oil and its major constituents was determined by topical application against S. zeamais.Results: A total of 30 components of the essential oil were identified. The major constituents were myristicin (41.3 %), elemicine (11.9 %), cis-isosafrole (11.5 %), borneol (9.1 %) and caryophyllene (8.8 %). The essential oil of A. fragrans exhibited contact toxicity against S. zeamais with LC50 value of 50.7 μg/adult. Elemicine possessed the strongest contact toxicity (LC50 = 13.5 μg/adult) while cis-isosafrole, myristicin, caryophyllene and borneol had LC50 values of 31.2 μg/adult, 43.4 μg/adult, 57.9 μg/adult, and 98.4 μg/adult, respectively.Conclusion: The study indicates that the essential oil of A. fragrans aerial parts and its major constituents have a potential for development into natural insecticides for the control of grain storage insects.Keywords: Ainsliaea fragrans, Sitophilus zeamais, Contact toxicity, Elemicine, Essential oil, Grain storage

Published
2015

19. Chemical Composition of Salvia plebeian R.Br. Essential Oil and its Larvicidal Activity against Aedes aegypti L

Author

Zhu, S, Liu, XC, Liu, ZL, and Xu, X

Subjects
Salvia plebeian, Essential oil, Larvicidal activity, Aedes aegypti, Caryophyllene oxide, γ- Eudesmol, τ-Cadinol, Calamenene
Abstract

Purpose: To evaluate the larvicidal activity of the essential oil of Salvia plebeian R.Br. (Labiatae) aerial parts against the larvae of Aedes aegypti L.Methods: The essential oil of S. plebeian aerial parts was obtained by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The essential oil was evaluated for larvicidal activity using World Health Organization (WHO) procedures, against the fourth larvae of A. aegypti within 24 h, and activity was recorded for various concentrations of the ranging from 12.5 – 200.0 μg/mL.Results: A total of 33 components of the essential oil of S. plebeian were identified. The major compounds of the essential oil were caryophyllene oxide (15.54 %), γ-eudesmol (14.03 %), τ-cadinol (10.21 %), calamenene (9.63 %), copaene (5.70 %), γ-cadinene (5.30 %), cadalene (5.28 %), α- muurolene (5.19 %), ledol (5.14 %) and α-cadinol (5.08 %). The essential oil exhibited larvicidal activity against A. aegypti at a median lethal concentration (LC50) of 46.26 μg/mL.Conclusion: The findings indicate that the essential oil of S. plebeian aerial parts has potentials for use in the control of A. aegypti larvae and may be useful in the search for newer, safer and more effective natural compounds as larvicides.Keywords: Salvia plebeian, Essential oil, Larvicidal activity, Aedes aegypti, Caryophyllene oxide, γ- Eudesmol, τ-Cadinol, Calamenene

Published
2015

20. Larvicidal Activity of Essential Oil Derived from Illicium henryi Diels (Illiciaceae) Leaf

Author

Liu, XC, Liu, QY, Zhou, L, and Liu, ZL

Subjects
Illicium henryi, Aedes albopictus, Larvicidal activity, Mosquito, Essential oil, Phenylpropanoids, Monoterpenoids, Sesquiternoids
Abstract

Purpose: To determine larvicidal activity of the essential oil derived from Illicium henryi Diels (Illiciaceae) leaf and stem against the larvae of Aedes albopictus Skuse．Methods: The essential oil of I. henryi leaves was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). The activity of the oil was evaluated, using World Health Organization (WHO) procedures, against the fourth instar larvae of A. albopictus for 24 h, and larval mortalities recorded at various essential oil concentrations ranging from 12.5 - 200 μg/mL.Results: A total of 25 components of the essential oil of I. henryi were identified and the principal compounds in the essential oil were safrole (54.09 %), myristicin (22.24 %), and 1, 8-cineole (5.43 %). The essential oil has higher content of (76.48 %) of phenylpropanoids than monoterpenoids (10.79 %) and sesquiternoids (11.72 %). The essential oil exhibited larvicidal activity against A. albopictus with a median lethal concentration (LC50) of 35.43 μg/mL.Conclusion: The findings obtained indicate that the essential oil of I. henryi leaves has potentials for use in the control of A. albopictus larvae and may be useful in the search for newer, safer and more effective natural compounds as larvicides.Keywords: Illicium henryi, Aedes albopictus, Larvicidal activity, Mosquito, Essential oil, Phenylpropanoids, Monoterpenoids, Sesquiternoids

Published
2015

21. Larvicidal activity of Illicium difengpi BN Chang (Schisandraceae) Stem Bark and its Constituent Compounds against Aedes aegypti L

Author

Liu, Y, Liu, XC, Liu, QY, Niu, C, and Liu, ZL

Subjects
Illicium difengpi, Aedes aegypti, Larvicidal activity, Myristicin, Safrole, 1,8-Cineole, Linalool
Abstract

Purpose: To determine the larvicidal activity of the essential oil derived from Illicium difengpi B.N. Chang stem bark (Schisandraceae) and its major constituents against the larvae of Aedes aegypti L.Methods: Essential oil of I. difengpi stem bark was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mas spectrometry (GC-MS). The activity of the essential oil and its major constituents was evaluated, using World Health Organization (WHO) procedures, against the fourth instar larvae of A. aegypti for 24 h, and larval mortalities recorded at essential oil/compound concentrations ranging from 6.0 - 200 μg/mL.Results: A total of 36 components of the essential oil of I. difengpi were identified. The principal compounds are safrole (18.21 %), linalool (13.47 %), 1,8-cineole (12.84 %), and myristicin (8.06 %) followed by α-terpineol (4.77 %), β-pinene (4.45 %) and 4-terpineol (4.38 %). The essential oil exhibited larvicidal activity against A. aegypti with LC50 (median lethal concentration) of 31.68 μg/mL. The major constituents, myristicin, safrole, and 1, 8-cineole, exhibited LC50 of 15.26, 39.45, and 72.18 μg/mL, respectively.Conclusion: The findings obtained indicate that the essential oil of I. difengpi and its major constituents have potentials for use in the control of A. aegypti larvae and may therefore be useful in the search for newer, safer and more effective natural compounds as larvicides.Keywords: Illicium difengpi, Aedes aegypti, Larvicidal activity, Myristicin, Safrole, 1,8-Cineole, Linalool

Published
2015

22. Insecticidal activity of essential oil of Cinnamomum cassia and its main constituent, trans-Cinnamaldehyde, against the booklice, Liposcelis bostrychophila

Author

Liu, XC, Cheng, J, Zhao, NN, and Liu, ZL

Subjects
Liposcelis bostrychophila, Cinnamomum cassis, Contact toxicity, Fumigant, trans- Cinnamaldehyde, Essential oil
Abstract

Purpose: To investigate the insecticidal activity of the essential oil of Cinnamomum cassis and its main constituent compound, trans-cinnamaldehyde, against the booklice, Liposcelis bostrychophila．Methods: Steam distillation of C. cassis twigs was carried out using a Clavenger apparatus in order to obtain the volatile oils. Gas chromatography/mass spectrometric (GC/MS) analyses (HP-5MS column) of the essential oil were performed and its contact (using impregnated filter paper method) and fumigant toxicity (sealed space) determined. The bioactive constituent compound, trans-cinnamaldehyde was isolated and identified from the oil based on bioactivity-directed fractionation.Results: A total of 35 components, accounting for 97.44 % of the essential oil of C. cassis, were identified. The principal compounds in the essential oil were trans-cinnamaldehyde (49.33 %), acetophenone (6.94 %), trans-cinnamic acid (5.45 %) and cis-cinnamaldehyde (4.44 %) followed by omethoxycinnamaldehyde (3.48 %), coumarin (3.42 %) and (E)-cinnamyl alcohol (3.21 %). The essential oil displayed contact toxicity against adult L. bostrychophila with a median lethal concentration (LC50) of 55.68 μg/cm2 as well as fumigant toxicity (LC50, 1.33 mg/l air). Trans-cinnamaldehyde exhibited strong contact and fumigant toxicity with LC50 and 1.29 mg/l air, respectively.Conclusion: The findings suggest that the essential oil of C. cassis and its constituent compound, trans-cinnamaldehyde, possess potentials for development into natural fumigants/insecticides for the control of booklice.Keywords: Liposcelis bostrychophila, Cinnamomum cassis, Contact toxicity, Fumigant, trans- Cinnamaldehyde, Essential oil

Published
2014

25. Larvicidal Activity of Isodon japonicus var. glaucocalyx (Maxim.) H.W.Li Essential Oil to Aedes aegypti L. and its Chemical Composition

Author

Liu, XC, Liu, QY, Zhou, L, and Liu, ZL

Subjects
Isodon japonicus var. glaucocalyx, Aedes aegypti, Larvicidal activity, Mosquito, Essential oil
Abstract

Purpose: To determine the larvicidal activity of the essential oil derived from Isodon japonicus var. glaucocalyx (Maxim.) H.W.Li (Labiatae) aerial parts at flowering stage against the larvae of Aedes aegypti L.Methods: The essential oil of I. japonicus var. glaucocalyx aerial parts was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mas spectrometry (GC-MS). The activity of the essential oil was evaluated, using World Health Organization (WHO) procedures, against the fourth larvae of A. aegypti for 24 h, and larval mortality recorded at various essential oil concentrations ranging from 12.5 - 200 µg/mL.Results: A total of 34 components of the essential oil of I. japonicus var. glaucocalyx were identified. The principal compounds of the essential oil were thujone (9.65 %), morillol (8.14 %), caryophyllene oxide (7.68 %), β-caryophyllene (7.60 %), α-terpineol (7.22 %), 1,8-cineole (7.09 %), linalool (5.56 %), Z-caryophyllene (5.10 %) and γ-eudesmol (4.71 %). The essential oil exhibited larvicidal activity against A. aegypti with a median lethal concentration (LC50) of 40.82 µg/mL.Conclusion: The findings indicate that the essential oil of I. japonicus var. glaucocalyx aerial parts has potentials for use in the control of A. aegypti larvae and may be useful in the search for newer, safer and more effective natural compounds for use as larvicides.Keywords: Isodon japonicus var. glaucocalyx, Aedes aegypti, Larvicidal activity, Mosquito, Essential oil

Published
2014

26. Chemical Composition and Insecticidal Activity of Essential Oil of Artemisia frigida Willd (Compositae) against Two Grain Storage Insects

Author

Liu, XC, Li, Y, Wang, T, Wang, Q, and Liu, ZL

Subjects
Artemisia frigida, Sitophilus zeamais, Liposcelis bostrychophila, Insecticidal activity, Essential oil, Cis-p-Menth-2-en-1-ol, 1,8-Cineole
Abstract

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Artemisia frigida against maize weevils (Sitophilus zeamais) and booklice (Liposcelis bostrychophila)DMethods: Steam distillation of A. frigida aerial parts was carried out in a Clavenger apparatus to extract its volatile oil content. Gas chromatography/mass spectrometric (GC/MS) analyses (HP-5MS column) ofthe essential oil were performed and its contact toxicity was determined using topical application and filter paper impregnation technique while its fumigant toxicity was evaluated using sealed-space method.Results: A total of 32 components of the essential oil were identified. The principal compounds were cis-ρ-menth-2-en-1-ol (20.8%), 1,8-cineole (12.0%), borneol (10.2%), lavandulol (9.3%), camphor (6.9%), and bicyclogermacrene (5.5%). The oil exhibited contact toxicity against adult S. zeamais and L. bostrychophila with LC50 value of 17.97 µg/adult and 254.38 µg/cm2, respectively. The essential oils also possessed fumigant toxicity against S. zeamais and L. bostrychophila with LC50 value of 69.46mg/L and 1.25 mg/L air.Conclusion: The study indicates that the essential oil of A. frigida has a potential to be developed to a natural fumigant/insecticide for the control of grain storage insects.Keywords: Artemisia frigida, Sitophilus zeamais, Liposcelis bostrychophila, Insecticidal activity, Essential oil, Cis-ρ-Menth-2-en-1-ol; 1,8-Cineole

Published
2014

27. Gas Chromatography-Mass Spectrometric Analysis of Essential Oil of Aerial Parts of Glycosmis parviflora (Sims) Little (Rutaceae)

Author

Liu, ZL, Yang, K, Bai, PH, Zhou, L, Liu, SL, and Liu, QZ

Subjects
Glycosmis parviflora, Essential oil, Meloidogyne incognita, Sitophilus zeamais, Triboliumcastaneum, Contact toxicity
Abstract

Purpose: To investigate the chemical composition and toxicity of the essential oil of Glycosmis parviflora (Sims) Little aerial parts against root-knot nematode and two grain storage insects (maize weevil and red flour beetle).Methods: Steam distillation of G. parviflora was carried out using Clavenger apparatus in order to extract its volatile oil. Gas chromatography/mass spectrometric (GC/MS) analysis (HP-5MS column) of the essential oil was performed and the toxicity of the oil determined by contact test.Results: A total of 37 components comprising 98.7 % of the essential oil were identified, of which (Z)- caryophyllene (20.6 %), methyl isoeugenol (11.1 %), (Z)-β-ocimene (8.9 %), α-cubebene (6.4 %), nerolidol (5.4 %), aromandendrene (4.9 %) and γ-pyronene (4.7 %) were found to be the major components. The essential oil possessed strong nematicidal activity against M. incognita with an LC50 value of 92.84 μg/ml. The essential oil of G. parviflora exhibited strong contact toxicity against S. zeamais and T. castaneum adults with LD50 values of 41.7 and 22.6 μg/adult, respectively.Conclusion: The study indicates that the essential oil of G. parviflora aerial parts has a potential for development into a natural insecticide/nematicide for control of nematodes and grain storage insects.Keywords: Glycosmis parviflora, Essential oil, Meloidogyne incognita, Sitophilus zeamais, Triboliumcastaneum, Contact toxicity

Published
2014

29. Essential Oil Composition and Larvicidal Activity of Clinopodium gracile (Benth) Matsum (Labiatae) Aerial Parts against the Aedes albopictus Mosquito

Author

Chen, XB, Liu, XC, Zhou, L, and Liu, ZL

Subjects
Clinopodium gracile, Aedes albopictus, Larvicidal activity, Mosquito, Essential oil
Abstract

Purpose: To determine larvicidal activity of essential oil derived from Clinopodium gracile (Benth.) Matsum. (Labiatae) aerial parts against the larvae of Aedes albopictus Skuse．Methods: Essential oil of C. gracile aerial parts was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The activity of the essential oil was evaluated, using World Health Organization (WHO) procedures, against the fourth larvae of A. albopictus for 24 h and larval mortality recorded at a various essential oil concentrations ranging from 12.5 – 200.0 μg/mL.Results: A total of 34 components of the essential oil of C. gracile were identified. The essential oil has higher content of sesquiterpenoids (70.49 %) than monoterpenoids (12.21 %). The other principal compounds of the essential oil were germacrene D (20.59 %), nootkatone (8.22 %), morillol (7.74 %), β- elemene (7.38 %), α-bergamotene (6.08 %), cis-β-farnesene (5.47 %) and caryophyllene (5.17 %). The essential oil exhibited larvicidal activity against A. albopictus with a median lethal concentration (LC50) of 42.56 μg/mL.Conclusion: The findings obtained indicate that the essential oil of C. gracile has potentials for use in the control of A. albopictus larvae and could be useful in the search for new, safer and more effective natural compounds as larvicides.Keywords: Clinopodium gracile, Aedes albopictus, Larvicidal activity, Mosquito, Essential oil

Published
2013

30. Chemical Composition and Insecticidal Activity of the Essential Oil of the Aerial Parts of Ostericum grosseserratum (Maxim) Kitag (Umbelliferae)

Author

Chu, SS, Liu, QZ, Du, SS, and Liu, ZL

Subjects
Ostericum grosseserratum, Sitophilus zeamais, Insecticidal, Contact toxicity, Fumigant, Essential oil
Abstract

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Ostericum grosseserratum against the maize weevil, Sitophilus zeamaisDMethods: Steam distillation of the aerial parts of O. grosseserratum during the flowering stage was carried out using a Clavenger apparatus in order to obtain its volatile oil content. Gas chromatography/mass spectrometric (GC/MS) analyses (HP-5MS column) of the essential oil were performed and its contact and fumigant activity determined.Results: A total of 43 components of the essential oil were identified. The principal compounds were (d)-limonene (16.2 %), 4-terpineol (13.5 %), myristicin (11.3 %), ƒÁ-terpineol (8.3 %), ƒÀ-pinene (5.1 %), ƒÀ-caryophyllene (4.6 %) and linalool (4.1 %). The oil exhibited contact toxicity against adult S. zeamais with lethal concentration (LC50) value of 17.97 ƒÊg/adult. The essential oils also possessed fumigant toxicity against S. zeamais with LC50 value of 13.70 mg/L air.Conclusion: The study indicates that the essential oil of O. grosseserratum has a potential to be developed into a natural fumigant/insecticide for the control of grain storage insects.Keywords: Ostericum grosseserratum; Sitophilus zeamais; Insecticidal, Contact toxicity; Fumigant; Essential oil

Published
2013

31. GC-MS Analysis of Insecticidal Essential Oil of Aerial Parts of Paederia scandens (Lour) Merrill (Rubiaceae)

Author

Yang, K, Liu, QZ, Liu, ZL, and Du, SS

Subjects
Paederia scandens, Sitophilus zeamais, Essential oil, Contact toxicity, Fumigant
Abstract

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Paederia scandens against the maize weevil, Sitophilus zeamais.Methods: Steam distillation of the aerial parts of P. scandens was carried out using Clavenger apparatus in order to obtain the volatile oils. Gas chromatography/mass spectrometric (GC/MS) analyses (HP-5MS column) of the essential oil were performed and its composition determined. Insecticidal activity of the essential oil was measured following topical application and seal-spaced fumigation.Results: The main components of the essential oil were β-pinene (24.77 %), α-pinene (8.22 %), 1, 8-cineol (8.01 %), δ-terpinene (5.32 %), (E)-β-ocimene (5.21 %), linalool (4.92 %) and 4-terpineol (3.63 %). The essential oil exhibited strong fumigant toxicity against adult S. zeamais with LC50 (lethal concentration at 50 %) value of 7.14 mg/L air. P. scandens essential oil also possessed contact toxicity against S. zeamais with LD50 (lethal dosage at 50 %) value of 47.78 µg/adult insect.Conclusion: The study indicates that the essential oil of P. scandens has a potential for development into a natural fumigant for control of insects in stored grains.

Published
2012

34. V 1162 Ori: A multiperiodic delta Scuti star with variable period and amplitude

Author

Arentoft, T Sterken, C Handler, G Freyhammer, LM Bruch, A Niarchos, P Gazeas, K Manimanis, V Van Cauteren, P and Poretti, E Dawson, DW Liu, ZL Zhou, AY Du, BT and Shobbrock, RR Garrido, R Fried, R Akan, MC Ibanoglu, C and Evren, S Tas, G Johnson, D Blake, C Kurtz, DW

Abstract

We present the results of multisite observations of the delta Scuti star V1162 Ori. The observations were done in the period October 1999 - May 2000, when 18 telescopes at 15 observatories were used to collect 253 light extrema during a total of 290 hours of time-series observations. The purpose of the observations was to investigate amplitude and period variability previously observed in this star, and to search for low-amplitude frequencies. We detect, apart from the main frequency and its two first harmonics, four additional frequencies in the light curves, all with low amplitudes (1-3 mmag). Combining the present data set with data obtained in 1998-99 at ESO confirms the new frequencies and reveals the probable presence of yet another pulsational frequency. All five low-amplitude frequencies are statistically significant in the data, but at least one of them (f(5)) suffers from uncertainty due to aliasing. Using colour photometry we find evidence for a radial main frequency (f(1)), while most or all low-amplitude frequencies are likely non-radial. We show that the main frequency of V1162 Ori has variable amplitude and period/phase, the latter is also displayed in the O-C diagram from light extrema. The amplitude variability in our data is cyclic with a period of 282 d and a range of nearly 20 mmag, but earlier amplitude values quoted in the literature cannot be explained by this cyclic variation. O-C analysis including data from the literature show that the period of V1162 Ori displays a linear period change as well as sudden or cyclic variations on a time scale similar to that of the amplitude variations.

Published
2001

46. Study on the reverse characteristics of Ti/6H-SiC Schottky contacts

Author

Shang Yc, Liu Zl, and Wang

Subjects
Materials science, Condensed matter physics, business.industry, Schottky barrier, Schottky effect, Electrical engineering, General Physics and Astronomy, Schottky diode, Thermionic emission, Metal–semiconductor junction, Field electron emission, business, Voltage drop, Quantum tunnelling
Abstract

The reverse I(V) measurement and analytic calculation of the electron transport across a Ti/6H-SiC Schottky barrier are presented. Based on the consideration of the barrier fluctuations and the barrier height shift caused by image charge and the applied voltage drop across Ti/SiC interfical layer, a comprehensive analytical model for the reverse tunneling current is developed using a WKB calculation of the tunneling probability through a reverse biased Schottky barrier. This model takes into account the main reverse conduction mechanism, such as field emission, thermionic field emission and thermionic emission. The fact that the simulated results are in good agreement with the experimental data indicates that the barrier height shift and barrier fluctuation can lead to reverse current densities orders of magnitude higher than that obtained from a simple theory. It is shown that the field and thermionic field emission processes, in which carries can tunnel through the barrier but cannot surmount it with insufficient thermal energy, dominate the reverse characteristics of a SiC Schottky contacts in a normal working condition.

Published
2003

49. Tropism mechanism of stem cells targeting injured brain tissues by stromal cell-derived factor-1.

Author

Zhang S, Liu XZ, Liu ZL, Shang CZ, Hu QL, Zhang, Sai, Liu, Xiao-zhi, Liu, Zhen-lin, Shang, Chong-zhi, and Hu, Qun-liang

Abstract

Objective: To explore the role and function of stromal cell-derived factor-1 (SDF-1) in stem cells migrating into injured brain area.Methods: Rat-derived nerve stem cells (NSCs) were isolated and cultured routinely. Transwell system was used to observe the migration ability of NSCs into injured nerve cells. Immunocytochemistry was used to explore the expression of chemotactic factor receptor-4 (CXCR-4) in NSCs. In vivo, we applied immunofluorescence technique to observe the migration of NSCs into injured brain area. Immunofluorescence technique and Western blotting were used to test expression level of SDF-1. After AMD3100 (a special chemical blocker) blocking CXCR-4, the migration ability of NSCs was tested in vivo and in vitro, respectively.Results: NSCs displayed specific tropism for injured nerve cells or traumatic brain area in vivo and in vitro. The expression level of SDF-1 in traumatic brain area increased remarkably and the expression level of CXCR-4 in the NSCs increased simultaneously. After AMD3100 blocking the expression of CXCR-4, the migration ability of NSCs decreased significantly both in vivo and in vitro.Conclusions: SDF-1 may play a key role in stem cells migrating into injured brain area through specially combining with CXCR-4. [ABSTRACT FROM AUTHOR]

Published
2009

50. Stem cells modified by brain-derived neurotrophic factor to promote stem cells differentiation into neurons and enhance neuromotor function after brain injury.

Author

Zhang S, Liu XZ, Liu ZL, Wang YM, Hu QL, Tie-Zhu M, Sun SZ, Zhang, Sai, Liu, Xiao-zhi, Liu, Zhen-lin, Wang, Yan-min, Hu, Qun-liang, Ma, Tie-zhu, and Sun, Shi-zhong

Abstract

Objective: To promote stem cells differentiation into neurons and enhance neuromotor function after brain injury through brain-derived neurotrophic factor (BDNF) induction.Methods: Recombinant adenovirus vector was applied to the transfection of BDNF into human-derived umbilical cord mesenchymal stem cells (UCMSCs). Enzyme linked immunosorbent assay (ELISA) was used to determine the secretion phase of BDNF. The brain injury model of athymic mice induced by hydraulic pressure percussion was established for transplantation of stem cells into the edge of injury site. Nerve function scores were obtained, and the expression level of transfected and non-transfected BDNF, proportion of neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP), and the number of apoptosis cells were compared respectively.Results: The BDNF expression achieved its stabilization at a high level 72 hours after gene transfection. The mouse obtained a better score of nerve function, and the proportion of the NSE-positive cells increased significantly (P<0.05), but GFAP-positive cells decreased in BDNF-UCMSCs group compared with the other two groups (P<0.05). At the site of high expression of BDNF, the number of apoptosis cells decreased markedly.Conclusion: BDNF gene can promote the differentiation of the stem cells into neurons rather than glial cells, and enhance neuromotor function after brain injury. [ABSTRACT FROM AUTHOR]

Published
2009

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