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215 results on '"Liu, Zongjun"'

5. Conformational variability of loops in the SARS-CoV-2 spike protein

Author

Wong, Samuel W. K. and Liu, Zongjun

Subjects
Quantitative Biology - Biomolecules, Statistics - Applications
Abstract

The SARS-CoV-2 spike (S) protein facilitates viral infection, and has been the focus of many structure determination efforts. Its flexible loop regions are known to be involved in protein binding and may adopt multiple conformations. This paper identifies the S protein loops and studies their conformational variability based on the available Protein Data Bank (PDB) structures. While most loops had essentially one stable conformation, 17 of 44 loop regions were observed to be structurally variable with multiple substantively distinct conformations based on a cluster analysis. Loop modeling methods were then applied to the S protein loop targets, and the prediction accuracies discussed in relation to the characteristics of the conformational clusters identified. Loops with multiple conformations were found to be challenging to model based on a single structural template., Comment: 24 pages

Published
2021

18. Wogonin alleviates BaP‐induced DNA damage and oxidative stress in human airway epithelial cells by dual inhibiting CYP1A1 activity and expression.

Author

Ni, Zhenhua, Ma, Honghong, Li, Xiaodong, Zou, Liwei, Liu, Zongjun, Wang, Xiongbiao, Ma, Hong, and Yang, Ling

Subjects
BENZOPYRENE, CYTOCHROME P-450 CYP1A1, DNA damage, EPITHELIAL cells, OXIDATIVE stress, METHOXY group
Abstract

Benzo[a]pyrene (BaP) is a common air pollutant that has been reported to cause oxidative stress and carcinogenesis. Wogonin, a flavonoid compound extracted from the roots of Scutellaria baicalensis, has been found to possess a variety of pharmacological activities, including anti‐inflammatory and anti‐cancer effects. The purpose of this study was to examine the ability of wogonin to alleviate the cytotoxicity induced by BaP in human airway epithelial cells and explore the corresponding mechanism. Our study found that wogonin treatment inhibited DNA damage and reactive oxygen species overproduction induced by BaP in human airway epithelial cells. In vitro enzyme assays showed that wogonin significantly inhibited the enzymatic activity of CYP1A1. In addition, wogonin decreased the basal level of CYP1A1 and inhibited the CYP1A1 overexpression induced by BaP, whereas overexpression of CYP1A1 partially reversed the effect of wogonin on BaP‐induced DNA damage. Meanwhile, a CYP1A1 inhibitor and CYP1A1 knockdown also showed these same effects. Further studies showed that wogonin regulates CYP1A1 expression by inhibiting CDK7 and CDK9 activity. The use of CDK7 or CDK9 inhibitors decreased BaP‐induced cytotoxicity and CYP1A1 expression. Finally, we found that the methoxy group of wogonin was crucial for its inhibitory activity. In conclusion, our data indicated that wogonin could effectively relieve BaP induced cytotoxicity, and its mechanism was related to the dual inhibition of CYP1A1 activity and expression. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Divergent indicator changes in different exercise states in non-hypertensive individuals and patients with hypertension

Author

Zhang, Hui, primary, Xu, Youlong, additional, Gao, Junqing, additional, Li, Min, additional, Fa, Jingjing, additional, Ding, Xinyue, additional, Yang, Chenhao, additional, Zhang, Lingxiao, additional, Luan, Yuling, additional, Xia, Yinjuan, additional, Wang, Mingyu, additional, Li, Qingli, additional, Xing, Lina, additional, and Liu, Zongjun, additional

Published
2023
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38. Effects of Intracoronary Pro-urokinase or Tirofiban on Coronary Flow During Primary Percutaneous Coronary Intervention for Acute Myocardial Infarction: A Multi-Center, Placebo-Controlled, Single-Blind, Randomized Clinical Trial

Author

Huang, Dong, primary, Qian, Juying, additional, Liu, Zongjun, additional, Xu, Yawei, additional, Zhao, Xianxian, additional, Qiao, Zengyong, additional, Fang, Weiyi, additional, Jiang, Li, additional, Hu, Wei, additional, Shen, Chengxing, additional, Liang, Chun, additional, Zhang, Qi, additional, and Ge, Junbo, additional

Published
2021
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44. Conformational variability of loops in the SARS‐CoV‐2 spike protein.

Author

Wong, Samuel W. K. and Liu, Zongjun

Abstract

The SARS‐CoV‐2 spike (S) protein facilitates viral infection, and has been the focus of many structure determination efforts. Its flexible loop regions are known to be involved in protein binding and may adopt multiple conformations. This article identifies the S protein loops and studies their conformational variability based on the available Protein Data Bank structures. While most loops had essentially one stable conformation, 17 of 44 loop regions were observed to be structurally variable with multiple substantively distinct conformations based on a cluster analysis. Loop modeling methods were then applied to the S protein loop targets, and the prediction accuracies discussed in relation to the characteristics of the conformational clusters identified. Loops with multiple conformations were found to be challenging to model based on a single structural template. [ABSTRACT FROM AUTHOR]

Published
2022
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50. Dalbergia odorifera extract promotes angiogenesis through upregulation of VEGFRs and PI3K/MAPK signaling pathways

Author

Youhua Wang, Zhu-Ming Fan, Liu Zongjun, Zhi-Xiu Lin, Ai-Lin Yang, Xin Zhou, Jian-Mei Yang, Yun-Xiao Lin, Su-yun Yuan, Min Cao, Hua Fan, and Wang Daying

Subjects
0301 basic medicine, MAPK/ERK pathway, Angiogenesis, Dalbergia, Neovascularization, Physiologic, Biology, Animals, Genetically Modified, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Western blot, Cell Movement, In vivo, Drug Discovery, Human Umbilical Vein Endothelial Cells, medicine, Animals, Humans, Therapeutic angiogenesis, Zebrafish, Cells, Cultured, PI3K/AKT/mTOR pathway, Cell Proliferation, Pharmacology, medicine.diagnostic_test, Plant Extracts, biology.organism_classification, Molecular biology, Cell biology, Receptors, Vascular Endothelial Growth Factor, 030104 developmental biology, 030220 oncology & carcinogenesis, cardiovascular system, Angiogenesis Inducing Agents, Mitogen-Activated Protein Kinases, Signal Transduction
Abstract

Ethnopharmacological relevance The heart wood of Dalbergia odorifera is a Chinese herbal medicine commonly used for the treatment of various ischemic diseases in Chinese medicine practice. Aim of the study In this study, therapeutic angiogenesis effects of the Dalbergia odorifera extract (DOE) were investigated on transgenic zebrafish in vivo and human umbilical vein endothelial cells (HUVECs) in vitro. Materials and methods The pro-angiogenic effects of DOE on zebrafish were examined by subintestinal vessels (SIVs) sprouting assay and intersegmental vessels (ISVs) injury assay. And the pro-angiogenic effects of DOE on HUVECs were examined by MTT, scratch assay, protein chip and western blot. Results In the in vivo studies, we found that DOE was able to dose-dependently promote angiogenesis in zebrafish SIVs area. In addition, DOE could also restore the injury in zebrafish ISVs area and upregulate the reduced mRNA expression of VEGFRs including kdr, kdrl and flt-1 induced by VEGF receptor kinase inhibitor II (VRI). In the in vitro studies, we observed that DOE promoted the proliferation, migration of HUVECs and also restored the injury induced by VRI. Moreover, protein chip and western blot experiments showed the PI3K/MAPK cell proliferation/migration pathway were activated by DOE. Conclusions DOE has a therapeutic effects on angiogenesis, and its mechanism may be related to adjusting the VEGFRs mRNA and activation of PI3K/MAPK signaling pathway. These results suggest a strong potential for Dalbergia odorifera to be developed as an angiogenesis-promoting therapeutic.

Published
2017

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