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249 results on '"Liu, Zi Wei"'

11. Imaging for Anterior Neck Trauma

Author

Tatla, Taranjit Singh, McIntyre, Charlotte, Mills, Heide, Liu, Zi Wei, Farrell, Roy, Tatla, Taranjit Singh, editor, Manjaly, Joseph, editor, Kumar, Raekha, editor, and Weller, Alex, editor

Published
2021
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14. Extraction and Structure Analysis of Wheat Bran Starch

Author

LI Xin, ZHANG Hong-wei, LIU Ying, GAO Fei, TANG Yan-jun, and LIU Zi-wei

Subjects
bran starch, ultrasonic, enzymatic method, particle structure, alkaline protease, radial crystal structure, a-type, Food processing and manufacture, TP368-456, Nutrition. Foods and food supply, TX341-641
Abstract

Wheat bran was taken as raw material to extract bran starch by alkaline protease method and ultrasonic-assisted alkaline protease method respectively. After extraction conditions of the two methods were optimized, the granular structure and molecular structure of bran starch were analyzed. The results showed that the optimal extraction conditions by alkaline protease method were as follows: solid-liquid ratio 1∶10 (g∶mL), enzyme dosage 529.92 U/mL, enzyme reaction time 50 min, reaction temperature 45 ℃and pH 12. The yield of starch was 30.40% and its content was 59.24%, while the powder was light brown;the optimal conditions by ultrasonic-assisted alkaline protease method were as follows: solid-liquid ratio 1∶15 g/mL, ultrasonic power 150 W and ultrasonic time 15 min. The yield of bran starch was 21.06% and its content was 90.64%, while the powder was white. The purity and extraction rate of bran starch extracted by ultrasonic-assisted alkaline protease method were better than those by single alkaline protease method.Analysis results showed that branch/straight ratio of the wheat bran starch was 4.69 and broken starch content was 1.78%. The starch bran particles were ellipsoidal, with an average particle size of 15.86 μm and radial crystal structure of A-type.

Published
2020
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23. Preoperative and postoperative optimisation of patients undergoing thyroid surgery: a multicentre quality improvement project at Barts Health NHS Trust

Author

Lechner, Matt, primary, Moghul, Gulwish, additional, Chandrasekharan, Deepak, additional, Ashraf, Salman, additional, Emanuel, Oscar, additional, Magos, Tiarnan, additional, Liu, Zi Wei, additional, Crutchlow, Margaret, additional, Kinghorn, Stephen, additional, McDonnell, Gayle, additional, McArdle, Harry, additional, Salem, Amr, additional, Papesch, Mike, additional, Majumdar, Kalpita, additional, Lakhdar, Abdulfattah, additional, Ali, Safina, additional, Ahmed, Jahangir, additional, Alusi, Ghassan, additional, Stimpson, Paul, additional, Waterhouse, Mona, additional, and Ghufoor, Khalid, additional

Published
2023
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32. Additional file 1 of Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin

Author

Sun, Bing-Yao, Sui, Hua-Lu, Liu, Zi-Wei, Tao, Xin-Yi, Gao, Bei, Zhao, Ming, Ma, Yu-Shu, Zhao, Jian, Liu, Min, Wang, Feng-Qing, and Wei, Dong-Zhi

Abstract

Additional file 1: Experimental details Tables S1−2; The ratio of indirubin and indigo in the mutants (Table S3); Steady-state kinetic parameters of bFMO and the mutants toward NADPH (Table S4); Comparing the crystal structures of 2XVE and 2XVJ (Fig. S1); The 96-well-plants for screening the mutants (Fig. S2); The characterizations of the mutants (Figs. S3−S6).

Published
2022
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36. Zuo Gui Wan Promotes Osteogenesis viaPI3K/AKT Signaling Pathway: Network Pharmacology Analysis and Experimental Validation

Author

Yang, Shuo, Zhang, Bin, Wang, Yu-guo, Liu, Zi-wei, Qiao, Bo, Xu, Juan, and Zhao, Li-sheng

Abstract

Objective: Osteogenesis is vitally important for bone defect repair, and Zuo Gui Wan (ZGW) is a classic prescription in traditional Chinese medicine (TCM) for strengthening bones. However, the specific mechanism by which ZGW regulates osteogenesis is still unclear. The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis. Methods: A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells (BMSCs). Results: In total, 487 no-repeat targets corresponding to the bioactive components of ZGW were screened, and 175 target genes in the intersection of ZGW and osteogenesis were obtained. And 28 core target genes were then obtained from a PPI network analysis. A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress, metal ions, and lipopolysaccharide. Additionally, KEGG pathway enrichment analysis revealed that multiple signaling pathways, including the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) signaling pathway, were associated with ZGW-promoted osteogensis. Further experimental validation showed that ZGW could increase alkaline phosphatase (ALP) activity as well as the mRNA and protein levels of ALP, osteocalcin (OCN), and runt related transcription factor 2 (Runx 2). What’s more, Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT, and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002. Finally, the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002. Conclusion: ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.

Published
2023
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