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This study experimentally investigated the effects of selected factors on the bending moment capacity (BMC) of case furniture joints. The main aim was to explore mixed applications of wood-based materials and fasteners in manufacturing case furniture to reduce material costs. The study examined the effects of the face member material—particle board (PB), plywood (PL), and block board (BB)—edge member material (PB, PL, and BB), and joint shape (T-shape and L-shape) on BMC. Additionally, the study evaluated the effects of joint type (two eccentrics (TE), two dowels (TD), and one eccentric and one dowel (ED)), and material type (PB, PL, and BB) on BMC for L-shaped joints. The results showed that joint shape and face member material significantly affected the BMC of case furniture joint. The BMCs of T-shaped joints were significantly greater than those of L-shaped joints, regardless of the material of the face and edge members, except when the face member was made of PL. For L-shaped joints with PL face members, the BMCs were significantly higher compared to others. Joints constructed with TE exhibited significantly higher BMC compared to ED and TD for the same material type. For PB, TE joints exhibited an increase of approximately 3.0 Nm and 2.0 Nm compared to TD and ED, respectively. For PL, TE showed an increase of 9.1 Nm and 4.1 Nm compared to ED and TD, respectively. For BB, the increases were 7.0 Nm and 6.6 Nm compared to ED and TD. The BMC of joints made with PL and constructed with TE and ED was significantly greater than those of BB, followed by PB. However, for joints assembled with TD, there was no significant difference among the three materials. The ratios of BMC for joints constructed with ED compared to the half-sum of TE and TD were 0.73, 1.04, and 0.79 for PB, PL, and BB, respectively. These results suggest that the face member material predominantly influences the BMC of case furniture joints, indicating the potential to reduce costs by combining different materials and joint types. [ABSTRACT FROM AUTHOR]

Surface suction provides an efficient way to delay boundary layer transitions. In order to verify the suction effects and determine the mechanism of suction control in transonic swept wing boundary layers, wind tunnel transition measurements in a hybrid laminar flow control (HLFC) wind tunnel model uses an infrared thermography technique in the Aircraft Research Association (ARA) 2.74 m × 2.44 m low turbulence level transonic wind tunnel. Based on the experimental data of stationary crossflow dominant transitions without and with surface suction in transonic swept wing boundary layers, in this paper, the effects on the receptivity and linear and nonlinear evolution of stationary crossflow vortices have been analyzed with the consideration of curvature. Theoretical analysis agreed with the experimental observations in regard to the transition delay caused by boundary layer suction near the leading-edge region. [ABSTRACT FROM AUTHOR]

In the efforts to explore an aptamer-based approach for target sensing and detection with higher sensitivity and specificity, instead of directly labeling aptamer with fluorophores, we proposed a new strategy by attaching a polymerase chain reaction (PCR) template to an oligonucleotide aptamer selected by systematic evolution of ligands by exponential enrichment (SELEX), so that after aptamer target binding, the template moiety serves as the PCR template in real-time quantitative PCR (RT-PCR), and therefore, the binding event can be reported by the following RT-PCR signals. Using the subtractive SELEX method, the oligonucleotide aptamers specific for the Fc fragment of mouse IgG were selected and subjected to coupling with the PCR dsDNA template by using overlap and the asymmetric extension PCR method. The target binding affinity of the PCR template tethered aptamer has been proven by electrophoretic mobility shift assay (EMSA), and further template tethered aptamer mediated real-time quantitative PCR (A-PCR) was conducted to validate the application for such a template tethered aptamer to be a sensitive probe for IgG detection. The results show that the protocols of A-PCR can detect 10-fold serial dilutions of the target, demonstrating a new mechanism to convert aptamer target binding events to amplified RT-PCR signal, and the feasibility of the PCR template tethered aptamer as a facile, specific, and sensitive target probing and detection is established. This new approach also has potential applications in multiple parallel target detection and analysis in a wide range of research fields.