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32. Circ ubiquitin‐like‐containing plant homeodomain and RING finger domains protein 1 increases the stability of G9a and ubiquitin‐like‐containing plant homeodomain and RING finger domains protein 1 messenger RNA through recruiting eukaryotic translation initiation factor 4A3, transcriptionally inhibiting PDZ and homeobox protein domain protein 1, and promotes the metastasis of hepatocellular carcinoma

Author

Huang, Xin, primary, Zhou, Li‐Zhi, additional, Feng, Wan‐Jiang, additional, Liu, Yu‐Qing, additional, Chen, Mi, additional, Tang, Lan‐Yan, additional, Gan, Ze‐Ying, additional, and Zhang, Pan, additional

Published
2023
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41. Circ ubiquitin‐like‐containing plant homeodomain and RING finger domains protein 1 increases the stability of G9a and ubiquitin‐like‐containing plant homeodomain and RING finger domains protein 1 messenger RNA through recruiting eukaryotic translation initiation factor 4A3, transcriptionally inhibiting PDZ and homeobox protein domain protein 1, and promotes the metastasis of hepatocellular carcinoma

Author

Huang, Xin, Zhou, Li‐Zhi, Feng, Wan‐Jiang, Liu, Yu‐Qing, Chen, Mi, Tang, Lan‐Yan, Gan, Ze‐Ying, and Zhang, Pan

Subjects
PROTEIN domains, HOMEOBOX proteins, MESSENGER RNA, HEPATOCELLULAR carcinoma, HIPPO signaling pathway, METASTATIC breast cancer
Abstract

Background and Aim: Circular ubiquitin‐like, containing PHD and ring finger domains 1 (circUHRF1) is aberrantly upregulated in human hepatocellular carcinoma (HCC) tissues. However, the underlying molecular mechanisms remain obscure. The present study aimed at elucidating the interactive function of circUHRF1–G9a–ubiquitin‐like, containing PHD and ring finger domains 1 (UHRF1) mRNA–eukaryotic translation initiation factor 4A3 (EIF4A3)–PDZ and LIM domain 1 (PDLIM1) network in HCC. Methods: Expression of circUHRF1, mRNAs of G9a, UHRF1, PDLIM1, epithelial–mesenchymal transition (EMT)‐related proteins, and Hippo–Yap pathway components was determined by quantitative polymerase chain reaction (Q‐PCR), immunofluorescence, or Western blot analysis. Tumorigenic and metastatic capacities of HCC cells were examined by cellular assays including Cell Counting Kit‐8, colony formation, wound healing, and transwell assays. Molecular interactions between EIF4A3 and UHRF1 mRNA were detected by RNA pull‐down experiment. Complex formation between UHRF1 and PDLIM1 promoter was detected by chromatin immunoprecipitation assay. Co‐immunoprecipitation was performed to examine the binding between UHRF1 and G9a. Results: Circular ubiquitin‐like, containing PHD and ring finger domains 1, G9a, and UHRF1 were upregulated, while PDLIM1 was downregulated in HCC tissue samples and cell lines. Cellular silencing of circUHRF1 repressed HCC proliferation, invasion, migration, and EMT. G9a formed a complex with UHRF1 and inhibited PDLIM1 transcription. Conclusion: Eukaryotic translation initiation factor 4A3 regulated circUHRF1 expression by binding to UHRF1 mRNA promoter. circUHRF1 increased the stability of G9a and UHRF1 mRNAs through recruiting EIF4A3. Overexpression of circUHRF1 aggravated HCC progression through Hippo–Yap pathway and PDLIM1 inhibition. By elucidating the molecular function of circUHRF1–G9a–UHRF1 mRNA–EIF4A3–PDLIM1 network, our data shed light on the HCC pathogenesis and suggest a novel therapeutic strategy for future HCC treatment. [ABSTRACT FROM AUTHOR]

Published
2024
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45. Genomic profiling and prognostic factors of H3 K27M-mutant spinal cord diffuse glioma

Author

Chai, Rui-Chao, Yan, Hao, An, Song-Yuan, Pang, Bo, Chen, Hui-Yuan, Mu, Quanhua, Zhang, Ke-Nan, Zhang, Yao-Wu, Liu, Yu-Qing, Liu, Xing, Zhao, Zheng, Jiang, Tao, Wang, Yong-Zhi, Jia, Wen-Qing, Chai, Rui-Chao, Yan, Hao, An, Song-Yuan, Pang, Bo, Chen, Hui-Yuan, Mu, Quanhua, Zhang, Ke-Nan, Zhang, Yao-Wu, Liu, Yu-Qing, Liu, Xing, Zhao, Zheng, Jiang, Tao, Wang, Yong-Zhi, and Jia, Wen-Qing

Abstract

H3 K27-altered diffuse midline glioma is a highly lethal pediatric-type tumor without efficacious treatments. Recent findings have highlighted the heterogeneity among diffuse midline gliomas with different locations and ages. Compared to tumors located in the brain stem and thalamus, the molecular and clinicopathological features of H3 K27-altered spinal cord glioma are still largely elusive, thus hindering the accurate management of patients. Here we aimed to characterize the clinicopathological and molecular features of H3 K27M-mutant spinal cord glioma in 77 consecutive cases. We found that the H3 K27M-mutant spinal cord glioma, with a median age of 35 years old, had a significantly better prognosis than H3 K27M-mutant brain tumors. We noticed a molecular heterogeneity of H3 K27M-mutant spinal cord astrocytoma via targeted sequencing with 34 cases. TP53 mutation which occurred in 58.8% of cases is mutually exclusive with PPM1D (26%) and NF1 (44%) mutations. The TP53-mutant cases had a significantly higher number of copy number variants (CNV) and a marginally higher proportion of pediatric patients (age at diagnosis <18 years old, p = 0.056). Cox regression and Kaplan–Meier curve analysis showed that the higher number of CNV events (≥3), chromosome (Chr) 9p deletion, Chr 10p deletion, ATRX mutation, CDK6 amplification, and retinoblastoma protein (RB) pathway alteration are associated with worse survival. Cox regression analysis with clinicopathological features showed that glioblastoma histological type and a high Ki-67 index (>10%) are associated with a worse prognosis. Interestingly, the histological type, an independent prognostic factor in multivariate Cox regression, can also stratify molecular features of H3 K27M-mutant spinal cord glioma, including the RB pathway, KRAS/PI3K pathway, and chromosome arms CNV. In conclusion, although all H3 K27M-mutant spinal cord diffuse glioma were diagnosed as WHO Grade 4, the histological type, molecular features represent

Published
2023

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