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Objective Gastrointestinal cancer is one of the most common malignant tumors in the world, and its incidence rate is always high. In recent years, research has shown that microorganisms may play a broad role in the diagnosis, pathogenesis, and treatment of cancer. Methods In this study, samples were first classified according to the microbial expression data of Gastrointestinal cancer, followed by functional enrichment and Immunoassay. In order to better understand the role of intratumor microorganisms in the prognosis, we screened gene signatures and constructed risk model through univariate cox and lasso regression and multivariable cox, then screened microbial signatures using zero-inflated model regression model and constructed risk index (RI), and finally predicted the immunotherapeutic effect of the risk model. Results The results indicate that the composition of tumor microorganisms in the C3 subtype is closely related to tumor angiogenesis, and there is a significant difference in the proportion of innate and acquired immune cells between the C2 and C1 subtypes, as well as differences in the physiological functions of immune cells. There are significant differences in the expression of microbial signatures between high and low risk subtypes, with 9 microbial signatures upregulated in high risk subtypes and 15 microbial signatures upregulated in low risk subtypes. These microbial signatures were significantly correlated with the prognosis of patients. The results of immunotherapy indicate that immunotherapy for high-risk subtypes is more effective. Conclusion Overall, we analyze from the perspective of microorganisms within tumors, pointing out new directions for the diagnosis and treatment of cancer. [ABSTRACT FROM AUTHOR]

A successful method of preparative high-speed counter-current chromatography (HSCCC) was established for direct isolation and purification of hydrolysis products ofEpimedium koreanumNakai to obtain the secondary glycosides and aglycons of flavonoids inE. koreanum. The two-phase solvent systems of HSCCC composed n-hexane-ethyl acetate-methanol-water at the ratios of 6:3.5:6:5 (v/v) and 6:3.5:8:5 (v/v) were used in a stepwise elution. Totally, 60.1 mg of FractionIthat would be isolated and purified by preparative HPLC, 9.4 mg of icartin (4), 3.5 mg of desmethylicartin (5), 4.7 mg of 3′′-ethyoxyl-icartin (6), 7.3 mg of anhydroicaritin (7), and 10.1 mg of β-anhydroicaritin (8) were obtained in one-step separation from 100 mg of the crude materials with purities of 97.7%, 96.2%, 99.2%, 99.5%, and 91.0%, respectively. And FractionIof HSCCC was purified by the preparative HPLC to obtain 12.7 mg of pletypetaloside (I-1), 6.8 mg of icariside I (I-2) and 16.3 mg of 3′′-ethyoxyl-pletypetaloside (I-3) with purities of 90.8%, 93.4%, and 99.2%. All of the chemical structures were identified on the basis of widely spectral technology including 2D-NMR. Among them, compoundsI-3and6are novel. [ABSTRACT FROM AUTHOR]

HB(N5)3M1∼2(N5)3BH (M = Be, Mg, Ca, Zn, and Cd) have been investigated as potential high-energy density materials in this article by means of density functional theory. They are all of high-energy content and show kinetic stability as to the breakup of N5 ring, especially HB(N5)3Mg(N5)3BH. The M&bond;N bondings in the mononuclear compounds are stronger than those in the corresponding dinuclear ones, except in the Be analogues. Furthermore, except HB(N5)3Zn2(N5)3BH, all the dinuclear compounds are shown to be energetically stable with respect to disproportionation. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2010 [ABSTRACT FROM AUTHOR]

Electrical power system forecasting has been a main focus for researchers who want to improve the effectiveness of a power station. Although some traditional models have been proved suitable for short-term electric load forecasting, its nature of ignoring the significance of parameter optimization and data preprocessing usually results in low forecasting accuracy. This paper proposes a short-term hybrid forecasting approach which consists of the three following modules: Data preprocessing, parameter optimization algorithm, and forecasting. This hybrid model overcomes the disadvantages of the conventional model and achieves high forecasting performance. To verify the forecasting effectiveness of the hybrid method, 30-minutes of electric load data from power stations in New South Wales and Queensland are used for conducting experiments. A comprehensive evaluation, including a Diebold-Mariano (DM) test and forecasting effectiveness, is applied to verify the ability of the hybrid approach. Experimental results indicated that the new hybrid method can perform accurate electric load forecasting, which can be regarded as a powerful assist in managing smart grids. [ABSTRACT FROM AUTHOR]

Objective: To establish a culture system for Psammosilene tunicoides hairy roots, and provide technological aid for the large-scale production of P. tunicoides material., Method: The young leaves and stem segments of sterile plantlets were infected with ACCC10060 strain, and subsequently a culture system suitable for hairy roots growth was further established., Result: When explants were co-cultured with ACCC10060 (A600 0.8) on B5 media containing 20 mg x L(-1) Acetosyringo (AS) for 48 h, the hairy roots could be successfully induced, and it could achieve a higher induction rate using young leaves as explants than that of stem segments. The transfected hairy roots possessed the ability of kanamycin resistance and growth on hormone-free media, and synthesis of opines. All above results demonstrated that the present hairy roots originated in the infection of P. tunicoides tissues by ACCC10060 strains. After 35 d culture in liquid hormone-free MS (1/2 strength), the biomass of hairy roots increased 14.11 times (fresh weight) and 8. 39 times (dry weight), respectively, and the content of total saponins in hairy roots reached to 0.857% (DW), by contrast, it's only 0.388% and 0.217% in callus and seedlings respectively., Conclusion: Establishment of hairy roots culture of P. tunicoides provided a foundation for industrial production of active components from P. tunicoides culture.