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17. DeepCristae, a CNN for the restoration of mitochondria cristae in live microscopy images.

Author

Papereux, Salomé, Leconte, Ludovic, Valades-Cruz, Cesar Augusto, Liu, Tianyan, Dumont, Julien, Chen, Zhixing, Salamero, Jean, Kervrann, Charles, and Badoual, Anaïs

Subjects
LIFE sciences, LIFE cycles (Biology), STIMULATED emission, CYTOLOGY, CELL anatomy
Abstract

Mitochondria play an essential role in the life cycle of eukaryotic cells. However, we still don't know how their ultrastructure, like the cristae of the inner membrane, dynamically evolves to regulate these fundamental functions, in response to external conditions or during interaction with other cell components. Although high-resolution fluorescent microscopy coupled with recently developed innovative probes can reveal this structural organization, their long-term, fast and live 3D imaging remains challenging. To address this problem, we have developed a CNN, called DeepCristae, to restore mitochondria cristae in low spatial resolution microscopy images. Our network is trained from 2D STED images using a novel loss specifically designed for cristae restoration. To efficiently increase the size of the training set, we also developed a random image patch sampling centered on mitochondrial areas. To evaluate DeepCristae, quantitative assessments are carried out using metrics we derived by focusing on the mitochondria and cristae pixels rather than on the whole image as usual. Depending on the conditions of use indicated, DeepCristae works well on broad microscopy modalities (Stimulated Emission Depletion (STED), Live-SR, AiryScan and LLSM). It is ultimately applied in the context of mitochondrial network dynamics during interaction with endo/lysosome membranes. DeepCristae is a convolutional network that reveals cristae in images taken with low photon illumination. It is a powerful tool to study cristae dynamics without compromising their structural integrity or functionality [ABSTRACT FROM AUTHOR]

Published
2025
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22. Gentle rhodamines for live-cell fluorescence microscopy

Author

Liu, Tianyan, primary, Kompa, Julian, additional, Ling, Jing, additional, Lardon, Nicolas, additional, Zhang, Yuan, additional, Chen, Jingting, additional, Reymond, Luc, additional, Chen, Peng, additional, Tran, Mai, additional, Yang, Zhongtian, additional, Zhang, Haolin, additional, Liu, Yitong, additional, Pitsch, Stefan, additional, Zou, Peng, additional, Wang, Lu, additional, Johnsson, Kai, additional, and Chen, Zhixing, additional

Published
2024
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37. Inside Cover: Red‐ and Far‐Red‐Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion (Angew. Chem. Int. Ed. 49/2021)

Author

Zhang, Junwei, primary, Peng, Xiaohong, additional, Wu, Yunxiang, additional, Ren, Huixia, additional, Sun, Jingfu, additional, Tong, Shiyan, additional, Liu, Tianyan, additional, Zhao, Yiwen, additional, Wang, Shusen, additional, Tang, Chao, additional, Chen, Liangyi, additional, and Chen, Zhixing, additional

Published
2021
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40. Combatiendo la desinformación: Cinco métodos para contrarrestar las teorías de conspiración en la pandemia de Covid-19

Author

Tianru, Guan, Liu, Tianyan, Randong, Yuan, Tianru, Guan, Liu, Tianyan, and Randong, Yuan

Abstract

Among the burgeoning discussions on the argumentative styles of conspiracy theories and the related cognitive processes of their audiences, research thus far is limited in regard to developing methods and strategies that could effectively debunk conspiracy theories and reduce the harmful influences of conspiracist media exposure. The present study critically evaluates the effectiveness of five approaches to reducing conspiratorial belief, through experiments (N=607) conducted on Amazon Mechanical Turk. Our results demonstrate that the content-based methods of counter conspiracy theory can partly mitigate conspiratorial belief. Specifically, the science- and fact-focused corrections were able to effectively mitigate conspiracy beliefs, whereas media literacy and inoculation strategies did not produce significant change. More crucially, our findings illustrate that both audience-focused methods, which involve decoding the myth of conspiracy theory and re-imagining intergroup relationships, were effective in reducing the cognitive acceptance of conspiracy theory. Building on these insights, this study contributes to a systematic examination of different epistemic means to influence (or not) conspiracy beliefs -an urgent task in the face of the infodemic threat apparent both during and after the COVID-19 pandemic., Entre las crecientes discusiones sobre los estilos argumentativos de las teorías de conspiración y los procesos cognitivos relacionados de su público, los estudios hasta ahora son limitados en lo que respecta al desarrollo de métodos y estrategias que podrían desacreditar eficazmente las teorías de conspiración y reducir las influencias dañinas de la exposición a los medios de comunicación conspirativos. El presente estudio evalúa de manera crítica la efectividad de cinco enfoques para reducir la creencia en conspiraciones, a través de experimentos (N=607) realizados en Amazon Mechanical Turk. Nuestros resultados demuestran que los métodos basados en el contenido al enfrentar las teorías de la conspiración pueden mitigar parcialmente la creencia conspiratoria. Específicamente, las correcciones centradas en la ciencia y los hechos fueron capaces de mitigar eficazmente las creencias en la conspiración, mientras que las estrategias de alfabetización mediática e inoculación no produjeron cambios significativos. Más importante aún, nuestros hallazgos ilustran que ambos métodos centrados en el público, que implican decodificar el mito de la teoría de la conspiración y reimaginar las relaciones intergrupales, fueron efectivos para reducir la aceptación cognitiva de la teoría de la conspiración. Basado en estos conocimientos, este estudio contribuye a un examen sistemático de distintos medios epistemológicos para influir (o no) en las creencias conspirativas, una tarea urgente frente a la evidente amenaza infodémica, tanto durante como después de la pandemia de COVID-19.

Published
2021

41. Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Author

Liu, Tianyan, primary, Zhang, Yu, additional, Cao, Yukai, additional, Jiang, Shan, additional, Sun, Rui, additional, Yin, Jiechao, additional, Gao, Zhenqiu, additional, Ren, Guiping, additional, Wang, Zhenzhong, additional, Yu, Qingzhong, additional, Sui, Guangchao, additional, Sun, Xu, additional, Sun, Wenying, additional, Xiao, Wei, additional, and Li, Deshan, additional

Published
2020
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42. The immune enhancement effects of recombinant NDV expressing chicken granulocyte-macrophage colony-stimulating factor on the different avian influenza vaccine subtypes

Author

Guo, Xiaochen, primary, Zhang, Teng, additional, Wang, Xiangxiang, additional, Su, Han, additional, Sun, Wenying, additional, Liu, Yunye, additional, Kang, Kai, additional, Liu, Tianyan, additional, Jiang, Shan, additional, Wang, Yaoqun, additional, Wang, Dan, additional, Yin, He, additional, Tian, Limin, additional, Li, Deshan, additional, and Ren, Guiping, additional

Published
2020
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44. Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Author

Liu, Tianyan, Zhang, Yu, Cao, Yukai, Jiang, Shan, Sun, Rui, Yin, Jiechao, Gao, Zhenqiu, Ren, Guiping, Wang, Zhenzhong, Yu, Qingzhong, Sui, Guangchao, Sun, Xu, Sun, Wenying, Xiao, Wei, and Li, Deshan

Abstract

The direct oncolytic effect of Newcastle disease virus (NDV) depends on the following two aspects: the susceptibility of cancer cells to virus infection and the ability of virus itself to lyse cancer cells. First, we investigate the susceptibility of cancer cells to NDV infection, HepG2, MDA-MB-231, and SH-SY5Y cells were susceptible, A549, MCF7, and LoVo cells were less susceptible. To investigate the molecular mechanism responsible for cancer cell susceptibility, transcriptome sequencing was carried out. We found that the levels of alpha-sialic acid acyltransferase were upregulated in MDA-MB-231 cells compared with MCF7 cells, and the interferon was downregulated. Second, to optimize the oncolytic capacity of the wild-type rClone30, a series of chimeric viruses rClone30-Anh(HN), rClone30-Anh(F), and rClone30-Anh(HN-F) were constructed by exchanging the HN gene, F gene or both of non-lytic rClone30 strain with lytic strain Anhinga. rClone30-Anh(F) and rClone30-Anh(HN-F) enhanced the oncolytic effect of the rClone30, and this enhancement is more obvious in the susceptible cells. The oncolytic mechanism of rClone30-Anh(F) was analyzed by transcriptome analyses, in comparison with rClone30, rClone30-Anh(F) upregulated the expression of ATG5, Beclin 1, and MAP1LC3B, thus activating autophagy and promoting the production of syncytia. In conclusion, our study provides a strategy to enhance the oncolytic effect of rClone30.

Published
2021
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48. NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy

Author

Tian, Xiaohe, primary, Liu, Tianyan, additional, Fang, Bin, additional, Wang, Aidong, additional, Zhang, Mingzhu, additional, Hussain, Sajid, additional, Luo, Lei, additional, Zhang, Ruilong, additional, Zhang, Qiong, additional, Wu, Jieying, additional, Battaglia, Giuseppe, additional, Li, Lin, additional, Zhang, Zhongping, additional, and Tian, Yupeng, additional

Published
2018
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49. Nitenpyram analogues with 1,4-dihydropyridine fixed cis-configuration:synthesis,insecticidal activities and molecular docking studies

Author

XUE Sijia, PANG Chuncheng, CHEN Yanxia, and LIU Tianyan

Subjects
synthesis, 1,4-dihydropyridine, insecticidal acvitity, molecular docking, lcsh:Science (General), cis-Nitenpyram analogue, lcsh:Q1-390
Abstract

A novel series of Nitenpyram analogues(Ia-Ij) with 1,4-dihydropyridine fixed cis-configuration were designed and synthesized.Preliminary bioassays showed that most of them exhibited good insecticidal activities against Aphis medicagini and Brown rice planthopper at 500 mg/L and 100 mg/L.The analogue Ij afforded the best activity in vitro,that had 100% mortality at 4 mg/L against Brown rice planthopper and Aphis medicagin.In addition,the molecular docking simulations revealed that the structural uniqueness of these analogues may lead to a unique molecular recognition and binding mode,and the results explained the SARs observed in vitro, which shed light on the novel insecticidal mechanism of these novel nitenpyam analogues.

Published
2013

50. Numerical Analysis of Electromagneto-Mechanical Coupling Dynamic Response of a Typical Tokomak Structure

Author

Liu Tianyan, Li Yong, Pei Cuixiang, Chen Zhenmao, Wu Wenjing, and Yuan Zhensheng (袁振圣)

Subjects
Coupling, Physics, Tokamak, Numerical analysis, Mechanics, Condensed Matter Physics, law.invention, Magnetic field, Classical mechanics, law, Electric field, Magnetic damping, Eddy current, Vector potential
Abstract

In an effort to simulate the dynamic behavior of a non-ferromagnetic conducting structure with consideration of the magnetic damping effect, a finite element code is developed, which is based on the reduced vector potential (Ar) method, the step-by-step integration algorithm and a time-partitioned strategy. An additional term is introduced to the conventional governing equations of eddy current problems to take into account the velocity-induced electric field corresponding to the magnetic damping effect. The TEAM-16 benchmark problem is simulated using the proposed method in conjunction with the commercial code ANSYS. The simulation results indicate that the proposed method has better simulation accuracy, especially in the presence of a high-intensity external magnetic field.

Published
2013
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