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11 results on '"Liu, Shoubai"'

3. Selective Effect of DNA N6-Methyladenosine Modification on Transcriptional Genetic Variations in East Asian Samples.

Author

Luan, Meiwei, Chen, Kaining, Zhao, Wenwen, Tang, Minqiang, Wang, Lingxia, Liu, Shoubai, Zhu, Linan, and Xie, Shangqian

Subjects
GENETIC variation, RNA modification & restriction, MOLECULAR biology, DNA methylation, GENETIC transcription regulation
Abstract

Genetic variations and DNA modification are two common dominant factors ubiquitous across the entire human genome and induce human disease, especially through static genetic variations in DNA or RNA that cause human genetic diseases. DNA N6-methyladenosine (6mA) methylation, as a new epigenetic modification mark, has been widely studied for regulatory biological processes in humans. However, the effect of DNA modification on dynamic transcriptional genetic variations from DNA to RNA has rarely been reported. Here, we identified DNA, RNA and transcriptional genetic variations from Illumina short-read sequencing data in East Asian samples (HX1 and AK1) and detected global DNA 6mA modification using single-molecule, real-time sequencing (SMRT) data. We decoded the effects of DNA 6mA modification on transcriptional genetic variations in East Asian samples and the results were extensively verified in the HeLa cell line. DNA 6mA modification had a stabilized distribution in the East Asian samples and the methylated genes were less likely to mutate than the non-methylated genes. For methylated genes, the 6mA density was positively correlated with the number of variations. DNA 6mA modification had a selective effect on transcriptional genetic variations from DNA to RNA, in which the dynamic transcriptional variations of heterozygous (0/1 to 0/1) and homozygous (1/1 to 1/1) were significantly affected by 6mA modification. The effect of DNA methylation on transcriptional genetic variations provides new insights into the influencing factors of DNA to RNA transcriptional regulation in the central doctrine of molecular biology. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Modern Technologies and Solutions to Enhance Surveillance and Response Systems for Emerging Zoonotic Diseases

Author

Zhang, Li, primary, Guo, Wenqiang, additional, Zhang, Yitian, additional, Liu, Shoubai, additional, Zhu, Zelin, additional, Guo, Meng, additional, Song, Wenxi, additional, Chen, Zhen, additional, Yang, Yiyi, additional, Pu, Yuqi, additional, Ding, Shiyu, additional, Zhang, Junkai, additional, Liu, Liu, additional, and Zhao, Qiwei, additional

Published
2023
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8. Swietenine extracted from Swietenia relieves myocardial hypertrophy induced by isoprenaline in mice

Author

Yanfang Yu, Feng Chen, Jiacheng Wang, Juejin Wang, Guanhong Xu, Qian Weichun, Liu Shoubai, Pingxi Xiao, Zhengsheng Mao, Kai Li, Jingjing Ding, Youjia Yu, and Chunyan Chu

Subjects
Limonins, Male, Cell Survival, Health, Toxicology and Mutagenesis, Cardiomegaly, 010501 environmental sciences, Management, Monitoring, Policy and Law, Pharmacology, Cell Enlargement, Toxicology, 01 natural sciences, Muscle hypertrophy, Cell Line, 03 medical and health sciences, Mice, 0302 clinical medicine, Western blot, In vivo, Diabetes mellitus, Isoprenaline, medicine, Animals, Myocytes, Cardiac, Meliaceae, Protein kinase B, 0105 earth and related environmental sciences, medicine.diagnostic_test, business.industry, Plant Extracts, Myocardium, Isoproterenol, Heart, General Medicine, Organ Size, medicine.disease, In vitro, Rats, Swietenia macrophylla, 030220 oncology & carcinogenesis, Seeds, business, medicine.drug
Abstract

As a traditional plant medicine in tropical areas, Swietenia macrophylla seeds are usually applied for some chronic diseases, including hypertension, diabetes, and so on. Few studies have been carried out to identify the effective elements in seed extract and their indications. In this study, we first investigated the functions of the swietenine, an extract from S. macrophylla seeds, using a model of myocardial hypertrophy induced by isoprenaline (ISO). At cellular level, H9c2 cell hypertrophy was also established through the treatment with ISO. The cardiac pathological remodeling was evaluated by echocardiography and histological analysis. Western blot and RT-qPCR were used to detect the expression of possible hypertrophy-promoting genes. Here, our results indicated that swietenine remarkably attenuated ISO-induced myocardial hypertrophy in vivo and in vitro. Moreover, Akt phosphorylation, ANP and BNP mRNA expression were efficiently decreased. Based on these findings, we concluded that swietenine might be a promising anti-hypertrophic agent against cardiac hypertrophy.

Published
2020

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