Search

Simple Summary: Epithelial-to-mesenchymal transition (EMT) plays a critical role in cancer progression, contributing to the invasive and migratory abilities of tumor cells. In this study, we show that FBXO11 promotes the degradation of ZEB1, a key EMT regulator, via ubiquitination. Loss of FBXO11 increases ZEB1 levels, enhancing the invasiveness of lung cancer cells, while its overexpression reduces ZEB1 and suppresses invasion. Importantly, higher FBXO11 expression is associated with better prognosis in non-small cell lung cancer (NSCLC), highlighting its potential role as a therapeutic target for controlling EMT and cancer metastasis. Epithelial-to-mesenchymal transition (EMT) affects the invasion and migration of cancer cells. Here, we show that FBXO11 recognizes and promotes ubiquitin-mediated degradation of ZEB1. There is a strong association between FBXO11 and ZEB1 in non-small cell lung cancer (NSLC) in a clinical database. FBXO11 interacts with ZEB1, a core inducer of EMT. FBXO11 leads to increased ubiquitination and proteasomal degradation of ZEB1. Depletion of endogenous FBXO11 causes ZEB1 protein accumulation and EMT in A549 and H1299 cells, while overexpression of FBXO11 reduces ZEB1 protein abundance and cellular invasiveness. Importantly, the depletion of ZEB1 suppresses the increased migration and invasion of A549 and H1299 cells promoted by the depletion of FBXO11. The same results are shown in xenograft tumors. High FBXO11 expression is associated with a favorable prognosis in NSLC. Collectively, our study demonstrates that FBXO11 modulates EMT by mediating the stability of ZEB1 in lung cancer cells. [ABSTRACT FROM AUTHOR]

The hormone auxin is involved in many biological processes throughout a plant's lifecycle. However, genes in the GH3 (Gretchen Hagen3) family, one of the three major auxin-responsive gene families, have not yet been identified in oilseed rape (Brassica napus). In this study, we identified 63 BnaGH3 genes in oilseed rape using homology searches. We analyzed the chromosome locations, gene structures, and phylogenetic relationships of the BnaGH3 genes, as well as the cis-elements in their promoters. Most BnaGH3 genes are located on chromosomes A03, A09, C02, C03, and C09, each with 4-7 members. In addition, we analyzed the expression patterns of BnaGH3 genes in seven tissues by transcriptome sequencing and quantitative RT-PCR analysis of plants under exogenous IAA treatment. The BnaGH3 genes showed different expression patterns in various tissues. BnaA.GH3.2-1 and BnaC.GH3.2-1 were expressed in the seed and seed coat during development and in response to IAA treatment. These results shed light on the possible roles of the GH3 gene family in oilseed rape. Key words: GH3, Brassica napus, expression analysis, auxin, stress response. L'auxine est une hormone impliquee dans plusieurs processus biologiques tout au long du cycle de vie d'une plante. Cependant, aucun gene GH3 (Gretchen Hagen3), une des trois principales familles de genes reagissant aux auxines, n'a encore ete identifie chez le colza (Brassica napus). Dans ce travail, les auteurs ont identifie 63 genes BnaGH3 sur la base de l'homologie. Les auteurs ont determine la position chromosomique, la structure genique et les relations phylogeniques entre ces genes ainsi que les elements cis dans leurs promoteurs. La plupart des genes BnaGH3 etaient situes sur les chromosomes A03, A09, C02, C03 et C09, chacun de ceux-ci comptant 4 a 7 membres. De plus, les auteurs ont analyse l'expression des genes BnaGH3 chez sept tissus par sequencage du transcriptome et par analyse RT-PCR quantitative de plantes exposees a un traitement a l'AIA exogene. Les genes BnaGH3 presentaient des expressions variees au sein des differents tissus. BnaA.GH3.2-1et BnaCGH3.2-1 etaient tous deux exprimes chez la graine et le tegument au cours de developpement et en reponse au traitement a l'AIA. Ces resultats jettent un eclairage sur les roles possibles des membres de la famille de genes GH3 chez le colza. [Traduit par la Redaction] Mots-cles : GH3, Brassica napus, expression genique, auxine, reponse aux stress., Introduction Auxin, a crucial phytohormone, plays roles in many aspects of plant growth and development, including cell elongation and division, light and gravity responses, root architecture determination, organ patterning, reproduction, [...]

Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. The genome of EHEC O157:H7 contains 177 unique O islands (OIs). Certain OIs significantly contribute to the heightened virulence and pathogenicity exhibited by EHEC O157:H7. However, the function of most OI genes remains unknown. We demonstrated here that EHEC O157:H7 adherence to and colonization of the mouse large intestine are both dependent on OI-97. Z3495, which is annotated as a LysR-type transcriptional regulator and encoded in OI-97, contributes to this phenotype. Z3495 activated the locus of enterocyte effacement (LEE) gene expression, promoting bacterial adherence. Deletion of z3495 significantly decreased the transcription of ler and other LEE genes, the ability to adhere to the host cells, and colonization in the mouse large intestine. Furthermore, the ChIP-seq results confirmed that Z3495 can directly bind to the promoter region of rcsF, which is a well-known activator of Ler, and increase LEE gene expression. Finally, phylogenetic analysis revealed that Z3495 is a widespread transcriptional regulator in enterohemorrhagic and enteropathogenic Escherichia coli. As a result of this study, we have gained a deeper understanding of how bacteria control their virulence and provide another example of a laterally acquired regulator that regulates LEE gene expression in bacteria. [ABSTRACT FROM AUTHOR]

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the most common cause of liver cirrhosis and cancer. Lonicerae flos polysaccharides (LPs) have been shown to be effective in treating metabolic diseases; however, the therapeutic effects and underlying molecular mechanisms of LPs in NAFLD remain unclear. PURPOSE: The objective of this study was to investigate the morphological characterization of Lonicerae flos polysaccharides (LPs) and the mechanism of LPs in relieving NAFLD. METHODS: The morphology of LPs was observed using atomic force microscopy (AFM), X‐ray diffraction (XRD), thermal weight (TG), and thermal weight derivative (DTG); NAFLD mice were treated with LPs at the same time as they were induced with a Western diet, and then the indexes related to glycolipid metabolism, fibrosis, inflammation, and autophagy in the serum and liver of the mice were detected. RESULTS: The atomic force microscope analysis results indicated that the LPs displayed sugar‐chain aggregates, exhibited an amorphous structure, and were relatively stable in thermal cracking at 150 °C. It was also found that LPs exerted therapeutic effects in NAFLD. The LPs prevented high‐fat and ‐cholesterol diet‐induced NAFLD progression by regulating glucose metabolism dysregulation, insulin resistance, lipid accumulation, inflammation, fibrosis, and autophagy. Adenosine 5′‐monophosphate (AMP)‐activated protein kinase (AMPK) inhibitor compound C abrogated LP‐induced hepatoprotection in mice with NAFLD. The LPs further treated NAFLD by reshaping the structure of the gut microbiota, in which Desulfovibrio bacteria plays a key roles. CONCLUSION: Lonicerae flos polysaccharides exert protective effects against NAFLD in mice by improving the structure of the intestinal flora and activating the AMPK signaling pathway. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Full‐color 3D printing technology has become more and more popular for industrial manufacturing applications. The voxelization of color 3D models and the expression of appearance colors become more and more important. To illustrate the complex appearance and internal characteristics of 3D models, we present a voxelization algorithm of the color 3D models for color 3D printing in this paper. Specifically, we use the 3D model of 3D Manufacturing Format (3MF) with color appearance features for surface voxelization first. Then, we propose an improved scan line filling algorithm to realize the voxelization of the interior of the model and obtain the color voxel model. Finally, to improve the high quality of color printing, we propose a surface color inward diffusion algorithm, which can make the surface color of the voxel model diffuse inward and further express the surface color of the voxel model. The experimental results show our proposed method can obtain an effective color voxel model and accurately express the color of the voxel model surface, which can enhance the color effect of the color model and realize the control of voxels. [ABSTRACT FROM AUTHOR]

With the development and progress of new energy electricity generation technology, a detailed study and test is required on new energy power generation equipment and micro-network system. As of the advantage of both numerical simulation and physical simulation, power connection type digital physical mixed simulation system could accomplish such research and tests. The simulation interface is the key to realise hybrid simulation. This paper proposes a current-mode damping impedance interface model based on adaptive impedance matching. The model is analysed from the interface stability, the accuracy and the dynamic response ability according to the structure of the interface model. Both theoretical analysis and simulation results show that current-mode damping impedance interface model based on adaptive impedance has high stability and accuracy, and also good dynamic response capability.

BACKGROUND: Hyperlipidemia is regarded as a public health matter, and its effective prevention and treatment are urgently required. However, the treatment of hyperlipidemia is still relatively scarce. RESULTS: Fermented Cerasus humilis fruit (FCHF) had higher total flavonoid, total phenolic, procyanidin, and organic and free amino acid content, and lower total sugar content, than non‐fermented C. humilis fruit (NFCHF). Both FCHF and NFCHF treatment significantly prevent putting on weight. Furthermore, FCHF administration ameliorated hyperlipidemia and cholesterol over‐accumulation. In addition, FCHF administration activated the antioxidase system and decreased the malondialdehyde content to relieve oxidative stress, and showed more efficaciously than NFCHF administration. FCHF treatments significantly reverse the fat deposition in high‐fat diet rat liver. FCHF supplementation can relieve the dysbacteriosis induced by hyperlipidemia, and regulate the composition of rat gut microbiota by increasing the abundance of Prevotella and norank_f_Muribaculaceae. CONCLUSION: Lactobacillus plantarum and Saccharomyces cerevisiae fermentation enhanced the antihyperlipidemic property of C. humilis fruits by promoting gut microbiota regulation. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]