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311 results on '"Liu, Qiu‐ning"'

19. Transcriptome Analysis Reveals Immune and Antioxidant Defense Mechanisms in the Eriocheir japonica sinensis after Exposure to Ammonia.

Author

Zhu, Xi-Rong, Jin, Ye, Zhang, Xue, Liu, Qiu-Ning, and Tang, Bo-Ping

Subjects
CHINESE mitten crab, OXIDANT status, ACID phosphatase, GENE expression, ALANINE aminotransferase
Abstract

Simple Summary: Ammonia poses a significant environmental hazard in aquaculture, with elevated concentrations exerting various detrimental effects on Chinese mitten crabs (Eriocheir japonica sinensis), including reduced growth rates, tissue damage, and increased mortality. This study aimed to investigate gene expression in the midgut of E. j. sinensis under ammonia exposure, and contribute to a more profound understanding of the species' energy metabolism and immune responses under such stress. The findings provide essential baseline data to support the sustainable development of the industry. As a key species in freshwater aquaculture, Eriocheir japonica sinensis was subjected to ammonia stress to assess its impact on the hepatopancreas. A total of 4007 differentially expressed genes (DEGs) were identified between control and treatment groups, comprising 1838 upregulated and 2169 downregulated genes. Following exposure to 300 mg/L of ammonia, the oxidative phosphorylation pathway was activated, while the lysosomal pathway was suppressed, thereby influencing immune functions. Thirteen DEGs from these pathways were further validated via qRT-PCR, revealing gene expression changes of one- to two-fold. Both acid phosphatase (ACP) and alkaline phosphatase (AKP) levels in the hepatopancreas and hemolymph initially increased and then decreased, indicating a disruption in immune functionality. Additionally, alanine transaminase (ALT) and triglyceride (TG) levels were measured, alongside catalase (CAT) activity, total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content, all of which showed an upward trend, signifying oxidative stress and tissue damage. These results offer critical insights into the antioxidant and immune mechanisms of E. j. sinensis in ammonia-enriched environments. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Transcriptome Analysis Reveals Antioxidant Defense Mechanisms in the Silkworm Bombyx mori after Exposure to Lead.

Author

Ye, Yang, Shi, Yan-Xia, Jiang, Qi, Jin, Ye, Chen, Fan-Xing, Tang, Wen-Hui, Peng, Qin, Liu, Qiu-Ning, Tang, Bo-Ping, and Wang, Jia-Lian

Subjects
LEAD exposure, SILKWORMS, ANTIOXIDANT analysis, HEAVY metal toxicology, GENE expression
Abstract

Simple Summary: Lead is a major source of heavy metal pollution, which can harm both the environment and people's health. High levels of Pb can slow down the growth and development of insects and may even cause them to die by damaging their cells. The aim of this study was to explore gene expression in the midgut of Bombyx mori when exposed to lead (Pb). This study gives us new insights into how insects protect themselves from Pb challenge and helps us understand the complex ways insects get rid of harmful substances at a deeper level. Lead (Pb) is a major source of heavy metal contamination, and poses a threat to biodiversity and human health. Elevated levels of Pb can hinder insect growth and development, leading to apoptosis via mechanisms like oxidative damage. The midgut of silkworms is the main organ exposed to heavy metals. As an economically important lepidopteran model insect in China, heavy metal-induced stress on silkworms causes considerable losses in sericulture, thereby causing substantial economic damage. This study aimed to investigate Pb-induced detoxification-related genes in the midgut of silkworms using high-throughput sequencing methods to achieve a deeper comprehension of the genes' reactions to lead exposure. This study identified 11,567 unigenes and 14,978 transcripts. A total of 1265 differentially expressed genes (DEGs) were screened, comprising 907 upregulated and 358 downregulated genes. Subsequently, Gene Ontology (GO) classification analysis revealed that the 1265 DEGs were distributed across biological processes, cellular components, and molecular functions. This suggests that the silkworm midgut may affect various organelle functions and biological processes, providing crucial clues for further exploration of DEG function. Additionally, the expression levels of 12 selected detoxification-related DEGs were validated using qRT-PCR, which confirmed the reliability of the RNA-seq results. This study not only provides new insights into the detoxification defense mechanisms of silkworms after Pb exposure, but also establishes a valuable foundation for further investigation into the molecular detoxification mechanisms in silkworms. [ABSTRACT FROM AUTHOR]

Published
2024
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