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Straw mulching cultivation technology can improve the soil environment of farmland, and it is applied in the dry farming area of Northwestern China. There are few studies on the effect of corn straw mulching on the soil temperature and yield of potato fields in dry land in Northwestern China. In this paper, three treatments, black film ridge (HM), corn straw mulching mechanized no-tillage planting (JG) and no-covering open field flat planting (CK), were set up in the period of 2022 to 2023. A field plot experiment was carried out to determine the soil temperature, growth index, and yield data during the key growth period. The statistical analysis results show that JG and HM significantly increased the potato yield, by 12.27~18.30% and 13.09~18.10%, compared with CK, but there was no significant difference between JG and HM. The yield was significantly positively correlated with tuber weight per plant at the tuber expansion stage, starch accumulation stage, and harvest stage (0.47 *~0.60 **), and significantly negatively correlated with the number of tubers at the harvest stage (−0.54 *). Compared with CK, HM increased the average soil temperature over the whole growth period by 0.27~0.92 °C. In 2022, the increase in the 5 cm soil layer in the tuber expansion period was the largest, reaching 0.83 °C. In 2023, the increase in the 5 cm soil layer in the starch accumulation period was the largest, reaching 3.08 °C. JG reduced the soil temperature over the whole growth period by 0.52 °C, and the 20 cm soil layer in the tuber formation period decreased the most, reaching 1.45 °C, which aggravated the soil temperature change over the whole growth period (the amplitude was 4.13~4.53 °C). The temperature difference between day and night in different growth periods in 2022 was 2.14~5.41 °C, and the soil temperature in some growth periods in 2022 even exceeded that with HM. The results showed that JG could regulate soil temperature and optimize the relationship between tuber weight per plant, tuber number per plant, and biomass allocation during tuber formation, which are beneficial for the improvement of the potato yield in the dry farming area of Northwestern China. [ABSTRACT FROM AUTHOR]

To explore the yield-increasing mechanism of mechanized potato planting with corn straw mulching, a two-year (2021 and 2022) field experiment was conducted to study the effects of mechanized no-tillage with straw mulching on potato yield and water use efficiency. This experiment included mechanized no-tillage potato planting with corn straw mulch covering (JG), plastic film mulching (HM), and open flat planting (CK). The results showed that mechanical no-tillage with straw mulching significantly affected soil water content in the 0–100 cm soil layer, yield, and water use efficiency (p < 0.05). There was no significant difference in yield between JG and HM, but it was significantly higher than that of CK. The yield of JG was 3.09~12.27% higher than that of CK. The yield increase was mainly achieved by increasing the potato weight per plant (0.697~0.862 **) and the average single potato weight (0.048~0.631). The tuber weight per plant was positively correlated with the plant height at the seedling stage (0.03~0.92 **) and positively correlated with the dry weight of stems and leaves at the tuber expansion stage and starch accumulation stage (0.74 **~0.95 **). It was negatively correlated with the number of branches at the tuber formation stage (−0.33~−0.88 **). Compared with CK, JG could significantly improve the water use efficiency of potatoes and reduce water consumption during the whole growth period of crops. In 2021, JG was 6.5% higher than CK, and HM was 6.88% lower than CK. In 2022, JG and HM increased water use efficiency by 26.17% and 14.50% compared with CK. When HM is applied in heavy soil areas, soil compaction can easily occur, which affects seedling emergence and reduces yield. At the same time, JG has strong adaptability to soil types and good yield stability. It can be seen that JG is a green and efficient mechanized potato cultivation technology suitable for dry farming areas. [ABSTRACT FROM AUTHOR]

RFC4 is required for DNA polymerase δ and DNA polymerase ε to initiate DNA template expansion. Downregulated RFC4 inhibits tumour proliferation by causing S‐phase arrest and inhibiting mitosis, resulting in the reduction of tumour cells. RFC4 has been implicated that it plays an important role in the initiation and progression of cancers, but a comprehensive analysis of the role of RFC4 in cancer has not been performed. We comprehensively analysed the expression, prognosis, methylation level, splicing level, relationship of RFC4 and immune infiltration, and pan‐cancer immunotherapy response used various databases (including TCGA, GTEx, UALCAN, Oncosplicing, TIDE, TISCH, HPA and CAMOIP), and experimented its biological function in HCC. Through pan‐cancer analysis, we found that RFC4 is significantly upregulated in most tumours. The tumour patients with high expression of RFC4 have poor prognosis. The methylation level and variable splicing level of RFC4 were abnormal in most tumours compared with the adjacent tissues. Furthermore, RFC4 was closely associated with immune cell infiltration in various cancers. RFC4 was significantly co‐expressed with immune checkpoints and other immune‐related genes. The expression of RFC4 could indicate the immunotherapy efficacy of some tumours. The RFC4 expression was associated with sensitivity to specific small molecule drugs. Cell experiments have shown that downregulated RFC4 can inhibit cell cycle and tumour cell proliferation. We conducted a systematic pan‐cancer analysis of RFC4, and the results showed that RFC4 can serve as a biomarker for cancer diagnosis and prognosis. These findings open new perspectives for precision medicine. [ABSTRACT FROM AUTHOR]

Atherosclerosis is an inflammatory disease. However, its etiology has not been yet fully elucidated. Endothelial dysfunction is currently considered to be one of the most important steps in the initiation of atherosclerosis. In addition, vascular smooth muscle cells, which are the main cellular component of de novo and in-stent restenosis lesions, play an important role in the development of atherosclerosis. Promoting the regeneration of endothelial cells and inhibiting the proliferation of smooth muscle cells are pivotal for the prevention and treatment of vascular injury. Recently, some studies have demonstrated that mesenchymal stem cells can home to the site of injury and differentiate into endothelial cells to repair damaged blood vessels. On the contrary, other researches have revealed that mesenchymal stem cells can differentiate into vascular smooth muscle cells that are involved in the development of restenosis. Here, we review the fundamental researches of mesenchymal stem cell therapy for atherosclerosis and address the perspectives of mesenchymal stem cells in atherosclerosis treatment.