Search

Your search keyword '"Liu, Meng-hua"' showing total 20 results
Start Over Author "Liu, Meng-hua"
20 results on '"Liu, Meng-hua"'

13. Codonopsis tangshenOliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin

Author

Lu, Xian-Yuan, Zhou, Feng-Hua, Dong, Ya-Qian, Gong, Lin-Na, Li, Qing-Yun, Tang, Lan, Cai, Zheng, He, Jing-Yu, and Liu, Meng-Hua

Abstract

Diabetic kidney disease (DKD) is the most serious microvascular complication during the development of diabetes with the characterizations of glomerular basement membrane thickening, mesangial expansion, and glomerular sclerosis, eventually leading to end-stage renal disease. This study aimed to investigate the melioration effect of Codonopisis tangshenOliv. (COD) on the DKD model, which was established by unilateral nephrectomy (UN)-high fat diet feeding (HFD) combined with streptozotocin (STZ). After the DKD rats were oral treated with COD at a dose of 2.7 mg/kg for 4 consecutive weeks, the blood glucose, lipid metabolism, renal function, inflammatory mediators, and fibrosis-associated proteins were examined. In vivo, the COD administration obviously relieved the weight loss, water intake, and blood glucose; decreased the total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels; and improved the renal function by reducing the expression of serum creatinine, uric acid, and urinary protein compared with the model group. The levels of pro-inflammatory cytokines of tumor necrosis factor-α, interleukin-1β, and IL-6 were significantly inhibited by COD. Meanwhile, the deposition of collagen fiber was markedly increased, and the protein and mRNA expressions of transforming growth factor-β1 and α-smooth muscle actin were markedly elevated in DKD rats, but they were decreased to some extent after the COD treatment. In conclusion, COD exhibited a protective effect on the UN-HFD feeding combined with STZ-induced DKD model by improving the blood glucose and lipid metabolism, relieving the inflammatory response, and mitigating the renal fibrosis, which provided scientific evidence for its applications in clinic.

Published
2018
Full Text
View/download PDF

18. Study on impurities in naringin extracted from Citrus grandis 'tomentosa'.

Author

Liu MH, Liu HB, Su WW, Liu XR, and Li JH

Subjects
Disaccharides chemistry, Flavanones analysis, Flavanones isolation & purification, Flavonoids chemistry, Fruit chemistry, Glycosides chemistry, Hypolipidemic Agents chemistry, Molecular Structure, Reproducibility of Results, Chromatography, High Pressure Liquid methods, Citrus chemistry, Disaccharides analysis, Flavanones chemistry, Flavonoids analysis, Glycosides analysis, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods
Abstract

Objective: To investigate the impurities in naringin extracted from Citrus grandis 'Tomentosa'., Methods: High performance liquid chromatographies coupled with photodiode array and electrospray ionization mass spectrometry detectors (HPLC-PDA/ESI-MS/MS) were applied to investigate the impurities, and their structures were elucidated by spectral data analyses. Quantification was carried out by main component self-compare with correction factor according to ICH guidelines., Results: Rhoifolin and neoeriocitrin were identified as major impurities. The correction factors of rhoifolin and neoeriocitrin were 1.82 and 1.02, respectively tested by HPLC method. The content of rhoifolin ranged from 0.742% to 0.926%, and the content of neoeriocitrin ranged from 0.335% to 0.464%. The gross impurities were less than 1.5%., Conclusion: The categories and quantities of impurities in naringin product are relatively stable. The research provides a way of specification and verification for the analysis of impurities and objective evidence for security assessment of naringin product.

Published
2012

19. Study on the interference of rat feed to pharmacokinetics of flavonoids.

Author

Yang CP, Liu MH, Zou W, Fang SQ, and Su Ww

Subjects
Animals, Citrus chemistry, Flavanones blood, Flavanones chemistry, Isoflavones blood, Isoflavones chemistry, Molecular Structure, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Electrospray Ionization methods, Animal Feed analysis, Chromatography, Liquid methods, Flavanones pharmacokinetics, Isoflavones pharmacokinetics
Abstract

Objective: To investigate the interference of rat feed to pharmacokinetic of flavonoids., Methods: Flavonoids in rat feed and plasma samples were separated by rapid resolution reversed-phase HPLC and characterized by LC-ESI-MS/MS analysis with an electrospray ionization source (ESI) and a triple quadrupole analyzer by fragmentation pattern., Results: Five Citrus flavonoids (naringin, naringenin, quercetin, hesperidin and hesperetin) and two soybean isoflavonoids (daizin,daidzein) were identified in common formula rat feed. The presence of these flavonoids in plasma from rats receiving the feed was also confirmed., Conclusion: The results showed the flavonoids in animal food might interfere with pharmacological and pharmacokinetic study of foreign natural compounds. This paper could be taken as a reference to pharmacological and pharmacokinetic studies of all the related substances.

Published
2011

20. [Studies on the alkaloids of Senecio scandens growing in Guangdong].

Author

Guo XF, Liu MH, Peng W, Wang YG, Yang CP, and Su WW

Subjects
Chromatography, High Pressure Liquid methods, Lactones analysis, Lactones chemistry, Monocrotaline analysis, Pyrrolizidine Alkaloids chemistry, Spectrometry, Mass, Electrospray Ionization methods, Plants, Medicinal chemistry, Pyrrolizidine Alkaloids analysis, Senecio chemistry
Abstract

Objective: To study alkaloids of Senecio scandens growing in Guangdong., Methods: The rapid resolution liquid chromatography-electrospray ionization mass spectrometry (RRLC-ESI-MS/MS) was used to analyse alkaloids of Senecio scandens growing in Guangdong, and senkirkine was isolated and purified by silica gel column chromatography., Results: Four alkaloids were identified as senkirkine, dehydrosenkirkine, monocrotaline and adonifoline, and senkirkine was firstly isolated from Senecio scandens growing in Guangdong., Conclusion: Senkirkine is the main component of Senecio scandens growing in Guangdong.

Published
2011

Catalog

Books, media, physical & digital resources
See catalog results