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26 results on '"Liu, Haiqiong"'

3. A Novel Role of Claudin-5 in Prevention of Mitochondrial Fission Against Ischemic/Hypoxic Stress in Cardiomyocytes

Author

Luo, Tao, Liu, Haiqiong, Chen, Baihe, Liu, Han, Abdel-Latif, Ahmed, Kitakaze, Masafumi, Wang, Xianbao, Wu, Yuanzhou, Chou, Dylan, and Kim, Jin Kyung

Subjects
Medical Physiology, Biomedical and Clinical Sciences, Cardiovascular, Heart Disease, Heart Disease - Coronary Heart Disease, Aetiology, 2.1 Biological and endogenous factors, Animals, Animals, Newborn, Apoptosis, Cells, Cultured, Claudin-5, Dynamins, GTP Phosphohydrolases, Hypoxia, Membrane Proteins, Microscopy, Electron, Transmission, Mitochondria, Heart, Mitochondrial Dynamics, Mitochondrial Proteins, Myocardial Reperfusion Injury, Myocytes, Cardiac, Rats, Rats, Sprague-Dawley, Cardiorespiratory Medicine and Haematology, Cardiovascular System & Hematology, Cardiovascular medicine and haematology
Abstract

BackgroundDownregulation of claudin-5 in the heart is associated with the end-stage heart failure. However, the underlying mechanism ofclaudin-5 is unclear. Here we investigated the molecular actions of claudin-5 in perspective of mitochondria in cardiomyocytes to better understand the role of claudin-5 in cardioprotection during ischemia.MethodsMyocardial ischemia/reperfusion (I/R; 30 min/24 h) and hypoxia/reoxygenation (H/R; 24 h/4 h) were used in this study. Confocal microscopy and transmission electron microscope (TEM) were used to observe mitochondrial morphology.ResultsClaudin-5 was detected in murine heart tissue and neonatal rat cardiomyocytes (NRCM). Its protein level was severely decreased after myocardial I/R or H/R. Confocal microscopy showedclaudin-5 presented in the mitochondria of NRCM. H/R-induced claudin-5 downregulation was accompanied by mitochondrial fragmentation. The mitofusin 2 (Mfn2) expressionwas dramatically decreased while the dynamin-related protein (Drp) 1 expression was significantly increased after H/R. The TEM indicatedH/R-induced mitochondrial swelling and fission. Adenoviral claudin-5 overexpression reversed these structural disintegration of mitochondria. The mitochondria-centered intrinsic pathway of apoptosis triggered by H/R and indicated by the cytochrome c and cleaved caspase 3 in the cytoplasm of NRCMs was also reduced by overexpressing claudin-5. Claudin-5 overexpression in mouse heart also significantly decreased cleaved caspase 3 and the infarct size in ischemic heart with improved systolic function.ConclusionWe demonstrated for the first time the presence of claudin-5 in the mitochondria in cardiomyocytes and provided the firm evidence for the cardioprotective role of claudin-5 in the preservation of mitochondrial dynamics and cell fate against hypoxia- or ischemia-induced stress.

Published
2021

5. Embeddings into almost self-centered graphs of given radius

Author

Xu, Kexiang, Liu, Haiqiong, Das, Kinkar Ch., and Klavžar, Sandi

Subjects
Mathematics - Combinatorics, 05C12, 05C05, 05C75
Abstract

A graph is almost self-centered (ASC) if all but two of its vertices are central. An almost self-centered graph with radius $r$ is called an $r$-ASC graph. The $r$-ASC index $\theta_r(G)$ of a graph $G$ is the minimum number of vertices needed to be added to $G$ such that an $r$-ASC graph is obtained that contains $G$ as an induced subgraph. It is proved that $\theta_r(G)\le 2r$ holds for any graph $G$ and any $r\ge 2$ which improves the earlier known bound $\theta_r(G)\le 2r+1$. It is further proved that $\theta_r(G)\le 2r-1$ holds if $r\geq 3$ and $G$ is of order at least $2$. The $3$-ASC index of complete graphs is determined. It is proved that $\theta_3(G)\in \{3,4\}$ if $G$ has diameter $2$ and for several classes of graphs of diameter $2$ the exact value of the $3$-ASC index is obtained. For instance, if a graph $G$ of diameter $2$ does not contain a diametrical triple, then $\theta_3(G) = 4$. The $3$-ASC index of paths of order $n\geq 1$, cycles of order $n\geq 3$, and trees of order $n\geq 10$ and diameter $n-2$ are also determined, respectively, and several open problems proposed.

Published
2017

6. Spexin inhibits excessive autophagy‐induced ferroptosis to alleviate doxorubicin‐induced cardiotoxicity by upregulating Beclin 1.

Author

Ou, Wen, Liu, Haiqiong, Chen, Changhai, Yang, Chaobo, Zhao, Xiaoqing, Zhang, Yu, Zhang, Zhiyin, Huang, Shuwen, Mo, Huaqiang, Lu, Weizhe, Wang, Xianbao, Chen, Aihua, Yan, Jing, and Song, Xudong

Subjects
*DOXORUBICIN, *CARDIOTOXICITY, *LIPID peroxidation (Biology), *MITOCHONDRIAL membranes, *LIPID metabolism
Abstract

Background and Purpose Experimental Approach Key Results Conclusion and Implications Doxorubicin is widely used in the treatment of malignant tumours, but doxorubicin‐induced cardiotoxicity severely limits its clinical application. Spexin is a neuropeptide that acts as a novel biomarker in cardiovascular disease. However, the effects of spexin on doxorubicin‐induced cardiotoxicity is unclear.We established a model of doxorubicin‐induced cardiotoxicity both in vivo and in vitro. Levels of cardiac damage in mice was assessed through cardiac function assessment, determination of serum cardiac troponin T and CKMB levels and histological examination. CCK8 and PI staining were used to assess the doxorubicin‐induced toxicity in cultures of cardiomyocytes in vitro. Ferroptosis was assessed using FerroOrange staining, determination of MDA and 4‐HNE content and ferroptosis‐associated proteins SLC7A11 and GPX4. Mitochondrial membrane potential and lipid peroxidation levels were measured using TMRE and C11‐BODIPY 581/591 probes, respectively. Myocardial autophagy was assessed by expression of P62 and Beclin1.Spexin treatment improved heart function of mice with doxorubicin‐induced cardiotoxicity, and attenuated doxorubicin‐induced cardiotoxicity by decreasing iron accumulation, abnormal lipid metabolism and inhibiting ferroptosis. Interestingly, doxorubicin caused excessive autophagy in cardiomyocyte in culture, which could be alleviated by treatment with spexin. Knockdown of Beclin 1 eliminated the protective effects of spexin in mice with DIC.Spexin ameliorated doxorubicin‐induced cardiotoxicity by inhibiting excessive autophagy‐induced ferroptosis, suggesting that spexin could be a drug candidate against doxorubicin‐induced cardiotoxicity. Beclin 1 might be critical in mediating the protective effect of spexin against doxorubicin‐induced cardiotoxicity. [ABSTRACT FROM AUTHOR]

Published
2024
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17. A novel function of ATF3 in suppression of ferroptosis in mouse heart suffered ischemia/reperfusion

Author

Liu, Haiqiong, primary, Mo, Huaqiang, additional, Yang, Chaobo, additional, Mei, Xiheng, additional, Song, Xudong, additional, Lu, Weizhe, additional, Xiao, Hua, additional, Yan, Jianyun, additional, Wang, Xianbao, additional, Yan, Jing, additional, Luo, Tao, additional, Lin, Yuhao, additional, Wen, Daojun, additional, Chen, Guiming, additional, Chen, Aihua, additional, and Ling, Yuanna, additional

Published
2022
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22. Research and Application of Secondary Equipment Management and Control System in Smart Grid

Author

Zhan Qingcai, Liu Haiqiong, Liu Quan, Zhang Shupei, Xu Cuijuan, Chen Lihui, and Xiong Hongying

Subjects
Information management, Engineering, business.industry, Control (management), computer.software_genre, Fault (power engineering), Maintenance engineering, Reliability engineering, Smart grid, Control system, Systems engineering, Information system, business, computer, Data integration
Abstract

Starting from the secondary equipment information management goal, through two equipment data integration and fault information system in production management system, build a control platform two equipment, equipment for the two time information of multi dimension analysis, improve the efficiency of operation and maintenance personnel. The two equipment control system has been successfully applied to the demonstration project site, and achieved good operating results.

Published
2017

23. Evaluation and Application of Relay Protection Based on Protection and Fault Information System

Author

Zhu Juepei, Zhan Qingcai, Liu Haiqiong, Peng Wenying, Ye Zhixiang, Sun Can, Zhu Hongjie, Li Jin, Chen Lihui, and Xu Cuijuan

Subjects
Engineering, business.industry, media_common.quotation_subject, Fault (power engineering), Automation, law.invention, Reliability engineering, Smart grid, Promotion (rank), Work (electrical), Relay, law, Information system, State (computer science), business, media_common
Abstract

With the rapid growth of the secondary equipment promotion and substation unattended substation, the traditional protection inspection and inspection work problems and difficulties are gradually emerging, which gradually changes to the state maintenance overhaul. In this paper, based on the trust system, the secondary equipment state evaluation scheme is designed, and the evaluation results provide the basis for the establishment of the secondary equipment maintenance plan.

Published
2017

24. Characteristics of Myocardial Structure and Central Carbon Metabolism during the Early and Compensatory Stages of Cardiac Hypertrophy

Author

Chen, Yuan, Luo, Yu-Mei, Li, Dong, Liu, Haiqiong, Luo, Xiaoqin, Zhang, Xinlei, Ling, Yuanna, and Ouyang, Wei

Abstract

Cardiac hypertrophy is a classical forerunner of heart failure and myocardial structural and metabolic remodeling are closely associated with cardiac hypertrophy. We aim to investigate the characteristics of myocardial structure and central carbon metabolism of cardiac hypertrophy at different stages. Using echocardiography and pathological staining, early and compensatory cardiac hypertrophy were respectively defined as within 7 days and from 7 to 14 days after transverse aortic constriction (TAC) in mice. Among mass-spectrometry-based metabolomics, we identified 45 central carbon metabolites. Differential metabolite analysis showed that six metabolites, including citrate, cis-aconitate and so on, decreased significantly on day 1 after TAC. Ten metabolites, including l-lactate, (S)-2-hydroxyglutarate and so on, were obviously changed on days 10 and 14. Pathway analysis showed that these metabolites were involved in seven metabolic pathways, including carbohydrates, amino acids and so on. Western blot showed the expression of ATP-citrate lyase, malate dehydrogenase 1 and lactate dehydrogenase A in myocardium changed markedly on day 3, while the phosphorylation level of AMP-activated protein kinase did not show significantly difference. We hope our research will promote deeper understanding and early diagnosis of cardiac hypertrophy in clinical practice. All raw data were deposited in MetaboLights (MTBLS10555).

Published
2024
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