Search

Your search keyword '"Liu, Aixian"' showing total 152 results
Start Over Author "Liu, Aixian"
152 results on '"Liu, Aixian"'

19. Circ_0001360 absence alleviates oxygen-glucose deprivation/reoxygenation-induced SK-N-SH cell injury via controlling the miR-671-5p/BMF pathway.

Author

Lu, Fang, Mo, Linhong, and Liu, Aixian

Subjects
ISCHEMIC stroke, CIRCULAR RNA, WOUNDS & injuries, WESTERN immunoblotting, CELL growth
Abstract

The regulatory potency of circular RNA (circRNA) has been acknowledged in multiple human diseases, including ischaemic stroke (IS). However, only a few circRNAs were investigated in this disorder. We aimed to uncover the role of circ_0001360 in cell models of IS in vitro. SK-N-SH cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to simulate IS pathology conditions in vitro. Quantitative real-time PCR (qPCR) and western blot were applied for expression detection. Cell viability, proliferation and apoptosis were investigated by CCK-8, EdU and flow cytometry assays. The predicted binding of miR-671-5p to circ_0001360 or BMF 3'UTR was validated by dual-luciferase reporter and RIP assays. Proteins on the NF-κB pathway were quantified by western blot to assess NF-κB pathway activity. Circ_0001360 was upregulated in SK-N-SH cells after OGD/R treatment. OGD/R provoked SK-N-SH cell growth impairment, apoptosis and inflammation, while circ_0001360 knockdown relieved these injuries. Circ_0001360 targeted miR-671-5p, and miR-671-5p deficiency recovered SK-N-SH cell injury that was repressed by circ_0001360 knockdown. MiR-671-5p directly combined with BMF and repressed BMF expression. Accordingly, circ_0001360 targeted miR-671-5p to regulate the expression of BMF. Circ_0001360 knockdown weakened the phosphorylated levels of P65 and IκBα, while further miR-671-5p deficiency or BMF overexpression restored their expression levels. Circ_0001360 contributed to OGD/R-caused SK-N-SH cell injury via targeting the miR-671-5p/BMF network and activating the NF-κB pathway, thus participating in the development of IS. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

42. Numerical Simulation of Effect of Structural Parameters on Cavitation Performance of Venturi Tube.

Author

DONG Bo, LUO Jingquan, ZHANG Jiahui, XU Zhen, LIU Aixian, SUN Qiang, WANG Yiwei, and GUO Xuqiang

Abstract

In order to achieve a better cavitation effect, the standard K-S turbulence model and the Schnerr-Sauer cavitation model were used to numerically simulate the cavitation flow fields of Venturi tubes based on FLUENT software. By comparing and analyzing the cavitation contours, vapor volume fraction and cavitation number obtained by simulation, the effects of structural parameters such as throat diameter to pipe diameter ratio, inlet angle, throat diameter to length ratio and outlet angle of Venturi tubes on cavitation were investigated. The results show that the cavitation effect firstly increases and then decreases along with the increase of throat diameter to pipe diameter ratio, and there is an optimum value. With the increase of inlet angle, the cavitation effect is weakened. With the increase of throat diameter to length ratio, the cavitation effect is enhanced. With the increase of outlet angle, the cavitation effect is weakened. The study results provide several references for the optimum design of Venturi tube cavitators. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results