3 results on '"Lisa Hallock"'
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2. ABSTRACTS FROM THE 2019 ANNUAL MEETING OF THE SOCIETY FOR NORTHWESTERN VERTEBRATE BIOLOGY, HELD JOINTLY WITH THE WASHINGTON CHAPTER OF THE WILDLIFE SOCIETY, AND IN ASSOCIATION WITH NORTHWEST PARTNERS IN AMPHIBIAN AND REPTILE CONSERVATION, AT GREAT WOLF LODGE, GRAND MOUND, WASHINGTON, 25 FEBRUARY–1 M
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Robyn P Angliss, Megan C Ferguson, Cara L Appel, Jeremy Brown, Claire Bortot, William T Bean, Katie M Moriarty, Sean M Matthews, David S Green, Stacy Anderson, Evan King, J Scott Yaeger, Ivan Arismendi, Stan Gregory, Randy Wildman, Linda Ashkenas, David Anderson, Laurie Shuster, Joseph R Evenson, Jessica L Huggins, John Calambokidis, Don Ashton, Scott Mcbain, Steve Railsback, R. Bruce Bury, Gwen W. Bury, C. Scott Baker, Angie Sremba, Logan Pallin, Shannon Atkinson, Andy Rogan, Iain Kerr, Jamie B. Bettaso, Justin M. Garwood, Ryan M. Bourque, Christopher J. West, Daniel M Bingham, Adam Sepulveda, Sally Painter, Evan M. Bredeweg, Tiffany S. Garcia, Anita T. Morzillo, Nathan Schumaker, Joseph B. Buchanan, Don T. Ashton, James Bettaso, David J. Germano, Frank Slavens, Kate Slavens, Gwendolynn W. Bury, Arianna Ilharreguy, Kiirsten Flynn, Gretchen Steiger, Elana Dobson, Mark Malleson, Brian Gisborne, Susan Berta, Alie Perez, Meghan Camp, Lisa A. Shipley, Johanna Varner, Tara Chestnut, Patrice K. Connors, Jessica E. Light, Brian P. Tanis, Joshua A. Drew, Chris N. Anderson, Anali M. Perry, Charon E. Henning, Mary Casillas, Katie Hinde, C. Toby St. Clair, Kyle Routledge, Charlie Palmer, Felix Martinez-Nunez, Christopher Cousins, Tiffany Garcia, Evan Bredeweg, Taal Levi, Jennifer Allen, Jeanne Dodds, Kristina Ernest, Erica Escajeda, Kate Stafford, Rebecca Woodgate, Kristin Laidre, Ann Froschauer, Bronwyn Hogan, Kimberly Dickerson, Jennifer Smith-Castro, Jonathan Reichard, Jeremy Coleman, James Goerz, Jared Oyster, Rich Harris, AJ Kroll, Claudine Reynolds, Josh Johnson, David Shaw, Caren S. Goldberg, Karen Pope, Nicolette Nelson, Jonah Piovia-Scott, Jessica R. Hale, Kristin L. Laidre, M. Tim Tinker, Ronald J. Jameson, Steven J. Jeffries, Shawn E. Larson, James L. Bodkin, Katherine Haman, Lisa Hallock, Lameace Kalisz, Ilai Keren, Jeff Harris, Patti Happe, Laura Hauck, Brooke Penaluna, Richard Cronn, Kevin Weitemier, Jessica A. Homyack, Matt Hane, Storm Beech, Michael J. Rochelle, Blake Hossack, Ken Honeycutt, Rebecca Mccaffery, Robin Russell, Iver T. Hull, Stephanie L. Berry, Chris Loggers, Timothy R. Johnson, Steven Jeffries, Dyanna Lambourn, Josh Oliver, Robert Delong, Sharon Melin, Pat Gearin, Tony Orr, Jeff Laake, Katie Jones, Glen P. Kalisz, Kelly Mcallister, Victoria Kaufman, Matthew Wilson, Tina Blewett, Travis King, Daniel Thornton, Jeffrey M. Kozma, Andrew J. Kroll, Jamie Thornton, Dyanna. M. Lambourn, Steve. J. Jeffries, Erin D'Agnese, Woutrina Smith, Kristin Wilkinson, Jessica Huggins, James Rice, Deborah Duffield, Michael Grigg, Stephen A. Raverty, Shawn Larson, Mark Leppin, R. B. Bury, Jeffrey C. Lewis, Jason Ransom, David Werntz, Anna O. Mangan, Jody C. Vogeler, Ian K. Breckheimer, Wendy M. King, Keith E. Bagnall, Katie M. Dugger, Brent M. Matsuda, Lorraine Andrusiak, Erin Clement, Purnima Govindarajulu, Katie Bell, Kurt Jenkins, Kim Sager-Fradkin, Thomas Mcintyre, Lisa Shipley, Stacey A. Nerkowski, Janet L. Rachlow, Lisette P. Waits, Stephanie M. Demay, Jon A. Gallie, Paul A. Hohenlohe, Jennifer R. Adams, Dawn P. Noren, Stephen Raverty, Joseph K. Gaydos, Judy A. ST. Leger, Gina M. Ylitalo, Stephen Nyman, Deanna H. Olson, Adrian Ares, Klaus J. Puettmann, Michael S. Parker, Kim M. Parsons, David S. Pilliod, Mark B. Hausner, Rick D. Scherer, Chad Mellison, Nathaniel D. Reynolds, Erik White, Stefanie Bergh, Eric Holman, Nicholle Stephens, James M. Wainwright, John Romansic, Matt Gray, Davis Carter, Deb Miller, Roger Rodriguez, Thomas J. Rodhouse, Pat Ormsbee, Kathryn Irvine, Jenny Barnett, Sarah Reif, Chris Rombough, Laura Trunk, Doug Sandilands, Hannah A. Sipe, Sarah J. Converse, Suzanne A. Stone, Stewart W. Breck, Jesse Timberlake, Peter M. Haswell, Fernando Najera, Brian S. Bean, Daniel J. Thornhill, Mira Sytsma, Laura Prugh, Beth Gardner, Tania Lewis, Austen C. Thomas, Jesse Howard, Phong Nguyen, Tracie A. Seimon, Kyle S. Tidwell, Brett A. Carrothers, Kristen N. Bayley, Lindsay N. Magill, Bjorn K. Van Der Leeuw, Andrew W. Trites, Jenny Urbina, Donelle Schwalm, Azzurra Valerio, Mariacristina Valerio, Luca Casadei, Matthew Vander Haegen, Christi Norman, Trina Bayard, Amanda Warlick, Eric Ward, Sandra O'Neill, Brad Hanson, Gina Ylitalo, Katy Weil, Logan Whiles, Jodi Wilmoth, Tlell Wolf, Jesse Short, Jay Bowerman, Christian Yarber, Caren Goldberg, Allan Pessier, Jesse Brunner, and Alexandre N. Zerbini
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Amphibian ,Geography ,biology ,biology.animal ,Wildlife ,General Earth and Planetary Sciences ,Vertebrate Biology ,Archaeology ,General Environmental Science - Published
- 2019
3. High-Throughput Amplification and Detection of Human Erythrocyte Antigen (HEA) Nucleotide Polymorphisms From Leukocyte Reduced Red Blood Cell (RBC) Units: Implications for Minor RBC Antigen Inventory Management by Hospital Transfusion Services
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Adelbert James, Xin Lin, Courtney K. Hopkins, Ermelina Enriquez, John D. Roback, Irene DeMezzo, Sheilagh Barclay, Cassandra D. Josephson, Robert M. Myers, Lisa Hallock, Christopher Tanzi, and Ghazala Hashmi
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business.industry ,Immunology ,Cell Biology ,Hematology ,Biochemistry ,Serology ,Red blood cell ,Leukoreduction ,medicine.anatomical_structure ,Antigen ,Genotype ,Medicine ,Multiplex ,business ,Genotyping ,Whole blood - Abstract
Abstract 3348 Background: Technologies have recently been developed for rapid determination of extended human erythrocyte antigen (xHEA) phenotypes. For example, a semi-automated method using allele-specific oligonucleotides targeted against 32 clinically significant minor RBC antigens has been used to determine donor xHEA phenotypes from whole blood samples. This approach is currently used by blood collection centers and medical centers with blood collection facilities (both sites have access to linked donor whole blood samples). Broader access to xHEA information closer to the point-of-care (e.g. Transfusion Services at a Medical Center without a blood collection facility) may provide an opportunity to enhance patient care by more quickly and broadly providing units with xHEA phenotypes (Klapper et al., 2010.) However, transfusion services would need to use integrally attached segments for testing, and with leukoreduced (LR) RBC units these segments have very low numbers of white blood cells (WBC) (and therefore DNA), potentially limiting analysis. This study was performed to determine whether a HEA-elongation mediated multiplex assay in solution (HEA-eMAP-S) (Xin et al., 2010) could accurately genotype segments from LR-RBC units for 32 clinically significant minor RBC antigens. Methods: Segments from pre-storage LR-RBC units (American Red Cross), < 14 days old, were obtained from a large tertiary care Children's Hospital in the Southeastern US and residual WBC were quantified by flow cytometry. DNA was extracted using an extraction method developed at BioArray SolutionS (BAS) using commercial reagents (Qiagen, Inc., Valencia, CA), and then amplified with the Universal Beadchip™ package (HEA LR-eMAP-S Beadchip™ Kits) which contains allele specific oligonucleotides directed to 32 clinically significant blood group antigens (c, C, e, E, V, VS, K, k, Kpa, Kpa, Jsa, Jsb, Jka, Jkb, Fya, Fyb, M, N, S, s, Lua, Lub, Dia, Dib, Coa, Cob, Doa, Dob, Joa, Hy, Yta, Ytb mutation for hemoglobin S). DNA analysis results were correlated with RBC storage solution, WBC filter type, and serologic minor RBC antigen phenotypes of the units. Results: 102 LR-RBC units from whole blood donations were studied, 74 /102 (73 %) stored in AS-1 and 28 /103 (27 %) in CPDA-1 solution. All AS-1 units were pre-storage LR with Fenwal Sepacell Flex Excel Filters and all CPDA-1 units were pre-storage LR with Whole Blood Fenwal Filters (Fenwal Inc. Lake Zurich, IL). All units demonstrated < 5 × 106 WBC/unit with 47 % having < 4 × 104 WBC/unit, which is at or below the limit of flow cytometric detection. Complete genotyping data was obtained from all samples. Ten samples showing initial indeterminate results on Diego and one for N antigens produced complete results after repeat testing. Fifty-four percent of units were serologically phenotyped for 1–8 antigens by the blood collection center; there was 100% correlation between predicted phenotype from DNA analysis and serology for these units. Conclusions: The HEA LR-eMAP-S DNA analysis can be applied to optimally pre-storage LR-RBC units yielding > 99 % accuracy for all minor red blood cell antigens tested. The ability to perform this type of testing in a hospital transfusion service opens up new possibilities for transfusion services to select from their existing inventory and more efficiently allocate units to recipients with specific phenotypic requirements for RBC units. Disclosures: Josephson: Immucor: Speakers Bureau. DeMezzo:Immucor: Employment. Tanzi:Immucor: Employment. Enriquez:Immucor: Employment. Lin:Immucor: Employment. Hashmi:Immucor: Employment.
- Published
- 2010
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