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Z-complementary pairs (ZCPs) have been widely used in different communication systems. In this paper, we first investigate the odd-periodic correlation property of ZCPs, and propose a new class of ZCPs, called ZOC-ZCPs with zero correlation zone (ZCZ) width \begin{document}$ Z $\end{document} and zero odd-period correlation zone (ZOCZ) width \begin{document}$ Z_{odd} = Z $\end{document} by horizontal concatenation of a certain combination of some known ZCPs. Particularly, based on any known Golay pair, we can generate a class of GCPs of more flexible length whose ZOCZ width is larger than a quarter of the sequence length.

Our previous study confirmed that miR-219-5p inhibits the progression of ovarian cancer (OC) by targeting high mobility group AT-hook 2 (HMGA2), while the role of miR-219-5p on the chemoresistance of OC is unclear. HMGA2 and miR-219-5p expression in OC tumors and various types of OC cells were determined by reverse transcription-quantitative PCR (RT-qPCR) and western blotting. The miRNA profiles in A2780 and cisplatin-resistant A2780 cells were investigated via bulk miRNA sequencing, and the interactions of miR-219-5p and HMGA2 were determined by luciferase reporter activity assay. Cell function was verified through Cell Counting Kit-8, invasion assay, wound-healing, and TUNEL assays. HMGA2 level is highly expressed in cisplatin-resistant OC cell lines compared to normal OC cells, while the expression trend of miR-219-5p is the opposite. In addition, we found that miR-219-5p is one of the miRNAs that have the most significant reduction in levels in the cisplatin-resistant A2780/DDP cell line compared to A2780 cells. Then, we reveal that miR-219-5p directly targets HMGA2 in cisplatin-resistant OC cells, and upregulation of miR-219-5p significantly reduces the resistance of OC cells to cisplatin both in vitro and in vivo. Finally, our results suggest that Wnt/β-catenin signaling and autophagy pathway is involved in the role of miR-219-5p/HMGA2 on resistance of OC cells to cisplatin via gain-of-function experiments. Collectively, the present study shows that miR-219-5p decreases the resistance of OC cells to cisplatin via Wnt/β-catenin signaling and autophagy by regulating HMGA2, which provides a feasible solution for the resistance of OC to chemotherapy.

Background Alcohol use disorders (AUD) has long been one of the most disability mental disorders and a major cause of health loss. Methods Based on open access data from the 2019 Global Burden of Disease (GBD 2019) study, we extracted data of years lived with disability (YLD), years of life lost (YLL) and disability-adjusted life years (DALY) to describe the changes of AUD burden over the period of 1990-2019 stratified by sex in globe, high-income countries (HICs), upper-middle income countries (UMCs), lower-middle income countries (LMCs), low-income countries (LICs) and China. We used Joinpoint regression model to fit the changing trend of years. And pairwise comparison was applied to test the coincidence parallelism and judge whether the difference of the trend among different regions is statistically significant. Results LMCs experienced the largest changes in the YLD rate of AUD from 1990 to 2019 (average annual percent change (AAPC) = -0.7, 95% confidence interval (CI) = -0.8, -0.7, P

Auricularia auricula polysaccharide (AAP) was isolated by hot-water extraction and purified to evaluate its structural and immunomodulatory effects on RAW264.7 macrophages. The results show that three kinds of Auricularia auricula polysaccharides (c-AAP) were obtained and named as AAP-I, AAP-II, and AAP-III Their further purification found that AAP-I and AAP-II were glycoproteins, and only AAP-III was a pure polysaccharide, which we named AAP for further experiments. Structural characteristics revealed that AAP was a homogeneous galactan comprising mannose, rhamnose, gluconic acid, glucose, galactose, arabinose, and fucose in a molar ratio of 5.02:0.9:0.12:4.48:0.37:1.0:0.36, and the average molecular weight is approximately 23.51 kDa. Methylation analysis revealed that AAP mainly consisted of 1,4-linked-Glcp, 1,4,6-linked-Glcp, terminal Glcp, 1,4-linked-Manp, 1,2,6-linked-Manp, and terminal Arap. Furthermore, the in vitro immunomodulatory activities of AAP were evaluated by cell proliferation, NO production, and phagocytic ability using RAW264.7 macrophage cells. The results show that AAP not only promoted the activation of macrophages but also provided a scientific basis for the further use of AAP. PRACTICAL APPLICATIONS: Three water-soluble polysaccharides were extracted from Auricularia auricula of Changbai Mountain, two of which contained binding proteins. Determination of molecular weight, structure, and immunoreactivity of pure polysaccharide components. The result clearly demonstrated the benefits of this plant as a healthy functional food.

Supplemental material, Supplementary_Material for Systematic review and meta-analysis of randomized controlled trials assessing the impact of Baduanjin exercise on cognition and memory in patients with mild cognitive impairment by Liqiang Yu, Fang Liu, Pingying Nie, Cuiling Shen, Junying Chen and Liqun Yao in Clinical Rehabilitation

Supplemental material, supplementary_Material for Effects of MOTOmed movement therapy on the mobility and activities of daily living of stroke patients with hemiplegia: a systematic review and meta-analysis by Cuiling Shen, Fang Liu, Liqun Yao, Zhongyuan Li, Li Qiu and Suzhu Fang in Clinical Rehabilitation

Grainy head (Grh) is a conserved transcription factor (TF) controlling epithelial differentiation and regeneration. To elucidate Grh functions, we identified embryonic Grh targets by ChIP-seq and gene expression analysis. We show that Grh controls hundreds of target genes. Repression or activation correlates with the distance of Grh binding sites to the transcription start sites of its targets. Analysis of 54 Grh-responsive enhancers during development and upon wounding suggests cooperation with distinct TFs in different contexts. In the airways, Grh repressed genes encode key TFs involved in branching and cell differentiation. Reduction of the POU-domain TF, Vvl, (ventral veins lacking) largely ameliorates the airway morphogenesis defects of grh mutants. Vvl and Grh proteins additionally interact with each other and regulate a set of common enhancers during epithelial morphogenesis. We conclude that Grh and Vvl participate in a regulatory network controlling epithelial maturation.

Metazoans have evolved efficient mechanisms for epidermal repair and survival following injury. Several cellular responses and key signaling molecules that are involved in wound healing have been identified in Drosophila, but the coordination of cytoskeletal rearrangements and the activation of gene expression during barrier repair are poorly understood. The Ret-like receptor tyrosine kinase (RTK) Stitcher (Stit, also known as Cad96Ca) regulates both re-epithelialization and transcriptional activation by Grainy head (Grh) to induce restoration of the extracellular barrier. Here, we describe the immediate downstream effectors of Stit signaling in vivo. Drk (Downstream of receptor kinase) and Src family tyrosine kinases bind to the same docking site in the Stit intracellular domain. Drk is required for the full activation of transcriptional responses but is dispensable for re-epithelialization. By contrast, Src family kinases (SFKs) control both the assembly of a contractile actin ring at the wound periphery and Grh-dependent activation of barrier-repair genes. Our analysis identifies distinct pathways mediating injury responses and reveals an RTK-dependent activation mode for Src kinases and their central functions during epidermal wound healing in vivo.

Grainy head (Grh) is a conserved transcription factor (TF) controlling epithelial differentiation and regeneration. To elucidate Grh functions we identified embryonic Grh targets by ChIP-seq and gene expression analysis. We show that Grh controls hundreds of target genes. Repression or activation correlates with the distance of Grh-binding sites to the transcription start sites of its targets. Analysis of 54 Grh-responsive enhancers during development and upon wounding suggests cooperation with distinct TFs in different contexts. In the airways, Grh-repressed genes encode key TFs involved in branching and cell differentiation. Reduction of the POU domain TF Ventral veins lacking (Vvl) largely ameliorates the airway morphogenesis defects of grh mutants. Vvl and Grh proteins additionally interact with each other and regulate a set of common enhancers during epithelial morphogenesis. We conclude that Grh and Vvl participate in a regulatory network controlling epithelial maturation.

Knowledge-elicitation is a common technique used to produce rules about the operation of a plant from the knowledge that is available from human expertise. Similarly, data-mining is becoming a popular technique to extract rules from the data available from the operation of a plant. In the work reported here knowledge was required to enable the supervisory control of an aluminium hot strip mill by the determination of mill set-points. A method was developed to fuse knowledge-elicitation and data-mining to incorporate the best aspects of each technique, whilst avoiding known problems. Utilisation of the knowledge was through an expert system, which determined schedules of set-points and provided information to human operators. The results show that the method proposed in this paper was effective in producing rules for the on-line control of a complex industrial process.

Alcoa Kitts Green has identified rolling longer plate as a key step to increase the capacity of hot mills. The two most problematic issues for rolling longer plates are: (1) to identify accurately the availability of power, and (2) to improve the rolled shape quality, e.g., reduce “banana-ing”. Models were developed to calculate the available power and a new tilt control formula was developed to deal with the issue of banana-ing. Trials were successfully completed to test the capability with increased rolling length. A 36% increase in hot mill capacity was achieved.

This paper describes the design, implementation and testing of an intelligent knowledge-based supervisory control (IKBSC) system for a hot rolling mill process. A novel architecture is used to integrate an expert system with an existing supervisory control system and a new optimization methodology for scheduling the soaking pits in which the material is heated prior to rolling. The resulting IKBSC system was applied to an aluminium hot rolling mill process to improve the shape quality of low-gauge plate and to optimise the use of the soaking pits to reduce energy consumption. The results from the trials demonstrate the advantages to be gained from the IKBSC system that integrates knowledge contained within data, plant and human resources with existing model-based systems.

The packing structures of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) thiolates on implicit gold surfaces were studied in explicit aqueous solutions of 1M NaCl using molecular dynamics simulations. The simulations were based on individual DNA chains placed in hexagonal simulation boxes of different sizes, representing various packing densities. The total potential energy per DNA chain was compared. The optimal packing structures were determined based on the minimal potential energy within the limits of the conditions that were evaluated in this study. The optimal packing density of ssDNA was found to be 0.19 DNA chains/nm(2), which is consistent with that determined experimentally. Furthermore, the optimal packing density of dsDNA was shown to be approximately 58% of the packing density for ssDNA, indicating that the packing of ssDNA should be approximately 58% of its optimal packing in order to achieve the best hybridization.

Results of the application of an advanced nonlinear controller to the problem of joint motion control for a robot are presented. For a class of desired state motions a control strategy is presented which ensures that the system asymptotically tracks the desired motion to any desired degree of accuracy. The results are applied to a three degree of freedom Tetrahedral robot. Simulation results and experimental studies are described.