1. A multicentre verification study of the QuantiFERON ® -TB Gold Plus assay.
- Author
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Pieterman ED, Liqui Lung FG, Verbon A, Bax HI, Ang CW, Berkhout J, Blaauw G, Brandenburg A, van Burgel ND, Claessen A, van Dijk K, Heron M, Hooghiemstra M, Leussenkamp-Hummelink R, van Lochem E, van Loo IHM, Mulder B, Ott A, Pontesilli O, Reuwer A, Rombouts P, Saegeman V, Scholing M, Vainio S, and de Steenwinkel JEM
- Subjects
- Adult, Belgium, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes microbiology, CD8-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes microbiology, Female, Host-Pathogen Interactions, Humans, Interferon-gamma metabolism, Latent Tuberculosis immunology, Latent Tuberculosis microbiology, Male, Middle Aged, Netherlands, Predictive Value of Tests, Reproducibility of Results, Antigens, Bacterial immunology, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Interferon-gamma immunology, Interferon-gamma Release Tests, Latent Tuberculosis diagnosis, Mycobacterium tuberculosis immunology
- Abstract
Objectives: The aim of this verification study was to compare the QuantiFERON
® -TB Gold Plus (QFT-Plus) to the QuantiFERON® -TB Gold In Tube (QFT-GIT). The new QFT-Plus test contains an extra antigen tube which, according to the manufacturer additionally elicits a CD8+ T-cell response above the CD4+ T-cell response. We assessed the value of this tube in detecting recent latent tuberculosis infections., Methods: Between May 2015 and December 2016, 1031 subjects underwent QFT-Plus and QFT-GIT test. Overall agreement between both tests and performance for different test indications and/or immune states was assessed. A difference of >0.6 IU/mL interferon-γ release between the two antigen tubes of the QFT-Plus assay was considered a true difference and used as estimation for CD8+ T-cell response., Results: Analysis of the QuantiFERON tests resulted in an overall agreement between assays of 95%. Subjects considered to be recently exposed to tuberculosis had significantly more often a true difference in interferon-γ release compared to all other subjects (p = 0.029)., Conclusion: Results of QFT-Plus are highly comparable to QFT-GIT. Although there is an indication that a true difference in interferon-γ release between the antigen tubes is associated with recent latent tuberculosis infection, the QFT-Plus could not be used to exclude recent exposure., (Copyright © 2017. Published by Elsevier Ltd.)- Published
- 2018
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