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4 results on '"Liqian Qin"'

1. Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

Author

Dongjiao Wang, Wei Wang, Shoujian Zang, Liqian Qin, Yanlan Liang, Peixia Lin, Yachun Su, and Youxiong Que

Subjects
Disease resistance, Expression profile, Transcriptome analysis, WRKY transcription factors, Agriculture, Agriculture (General), S1-972
Abstract

WRKY transcription factors, transcriptional regulators unique to plants, play an important role in defense response to pathogen infection. However, the resistance mechanisms of WRKY genes in sugarcane remain unclear. In the present study, gene ontology (GO) enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense response. We identified gene ScWRKY4, a class IIc member of the WRKY gene family, in sugarcane cultivar ROC22. This gene was induced by salicylic acid (SA) and methyl jasmonate (MeJA) stress. Interestingly, expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smut-susceptible sugarcane cultivars infected with Sporisorium scitamineum. Moreover, stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var. coeruleum and caused down-regulated expression of immune marker-related genes. Transcriptome analysis indicated suppressed expression of most JAZ genes in the signal transduction pathway. ScWRKY4 interacted with ScJAZ13 to repress its expression. We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance, most likely through the JA signaling pathway. The present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.

Published
2024
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2. Identification and characterization of WAK gene family in Saccharum and the negative roles of ScWAK1 under the pathogen stress

Author

Dongjiao Wang, Liqian Qin, Mingxing Wu, Wenhui Zou, Shoujian Zang, Zhennan Zhao, Peixia Lin, Jinlong Guo, Hengbo Wang, and Youxiong Que

Subjects
Structural Biology, General Medicine, Molecular Biology, Biochemistry
Abstract

Wall-associated kinase (WAK) is widely involved in signal transduction, reproductive growth, responses to pathogen infection and metal ion stress in plants. In this study, 19, 12, and 37 SsWAK genes were identified in Saccharum spontaneum, Saccharum hybrid and Sorghum bicolor, respectively. Phylogenetic tree showed that they could be divided into three groups. These WAK genes contained multiple cis-acting elements related to stress, growth and hormone response. RNA-seq analysis demonstrated that SsWAK genes were constitutively expressed in different sugarcane tissues and involved in response to smut pathogen (Sporisorium scitamineum) stress. Additionally, ScWAK1 (GenBank Accession No. OP479864), was then isolated from sugarcane cultivar ROC22. It was highly expressed in leaves and roots and its expression could be induced under SA and MeJA stress. Besides, ScWAK1 was significantly downregulated in both smut-resistant and susceptible sugarcane cultivars in response to S. scitamineum infection. ScWAK1 was a membrane protein without self-activating activity. Furthermore, transient expression of ScWAK1 in Nicotiana benthamiana enhanced the susceptibility of tobacco to the inoculation of Ralstonia solanacearum and Fusarium solani var. coeruleum, suggesting its negative role in disease resistance. The present study reveals the origin, distribution and evolution of WAK gene family and provides potential gene resources for sugarcane molecular breeding.

Published
2022

3. Genome-Wide Identification of Auxin-Responsive

Author

Wenhui, Zou, Peixia, Lin, Zhennan, Zhao, Dongjiao, Wang, Liqian, Qin, Fu, Xu, Yachun, Su, Qibin, Wu, and Youxiong, Que

Subjects
Plant Breeding, Indoleacetic Acids, Gene Expression Regulation, Plant, Salicylic Acid, Plants, Genetically Modified, Saccharum, Plant Proteins
Abstract

Gretchen Hagen3 (

Published
2022

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