179 results on '"Liping, Guan"'
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2. A strategy to reconstitute immunity without GVHD via adoptive allogeneic Tscm therapy
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Liping Guan, Yunqin Sun, Yanli Si, Qingya Yan, Ziyu Han, Youxun Liu, and Tao Han
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T memory stem cells ,graft-versus-host disease ,alloreactive T cells ,adoptive immunotherapy ,immune reconstitution ,Immunologic diseases. Allergy ,RC581-607 - Abstract
IntroductionAdoption of allogeneic T cells directly supplements the number of T cells and rapidly induces T-cell immunity, which has good efficacy for treating some tumors and immunodeficiency diseases. However, poor adoptive T-cell engraftment and graft-versus-host disease (GVHD) limit the application of these methods. Alloreactive T-cell clones were eliminated from the donor T-cell repertoire, and the remaining T-cell clones were prepared as Tscm for T-cell adoptive treatment to reconstruct recipient T-cell immunity without GVHD.MethodsThe subjects in this study included three different strains of mice. Lymphocytes from mice (C57BL/6) were used as the donor T-cell repertoire, from which the Tscm allo-reactive T cell clone was depleted (ATD-Tscm). This was confirmed by showing that the Tscm was not responsive to the alloantigen of the recipient (BALB/c). To prepare ATD-Tscm cells, we used recipient lymphocytes as a simulator, and coculture of mouse and recipient lymphocytes was carried out for 7 days. Sorting of non-proliferative cells ensured that the prepared Tscm cells were nonresponsive. The sorted lymphocytes underwent further expansion by treatment with TWS119 and cytokines for an additional 10 days, after which the number of ATD-Tscm cells increased. The prepared Tscm cells were transferred into recipient mice to observe immune reconstitution and GVHD incidence.ResultsOur protocol began with the use of 1×107 donor lymphocytes and resulted in 1 ×107 ATD-Tscm cells after 17 days of preparation. The prepared ATD-Tscm cells exhibited a nonresponse upon restimulation of the recipient lymphocytes. Importantly, the prepared ATD-Tscm cells were able to bind long and reconstitute other T-cell subsets in vivo, effectively recognizing and answering the “foreign” antigen without causing GVHD after they were transferred into the recipients.DiscussionOur strategy was succeeded to prepare ATD-Tscm cells from the donor T-cell repertoire. The prepared ATD-Tscm cells were able to reconstitute the immune system and prevent GVHD after transferred to the recipients. This study provides a good reference for generating ATD-Tscm for T-cell adoptive immunotherapy. more...
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- 2024
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3. EGCG induces degradation of active folate in serum via H2O2 generation, while L-ascorbic acid effectively reverses this effect
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Guangbin Zhou, Mengmeng Zhang, Xiaoyu Sun, Ting Huang, Kun Hou, Siqi Zhou, Jun Yin, and Liping Guan
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Epigallocatechin gallate ,5-methyltetrahydrofolate ,L-ascorbic acid ,Hydrogen peroxide ,Pro-oxidant ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Empirical studies have indicated that excessive tea consumption may potentially decrease folate levels within the human body. The main active component in green tea, epigallocatechin gallate (EGCG), significantly reduces the concentration of 5-methyltetrahydrofolate (5-MTHF) in both solution and serum. However, our findings also demonstrate that the pro-degradation effect of EGCG on 5-MTHF can be reversed by L-ascorbic acid (AA). Subsequent investigations suggest that EGCG could potentially expedite the degradation of 5-MTHF by generating hydrogen peroxide. In summary, excessive tea intake may lead to reduced folate levels in the bloodstream, yet timely supplementation of AA could potentially safeguard folate from degradation. more...
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- 2024
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4. Non-invasive prediction of preeclampsia using the maternal plasma cell-free DNA profile and clinical risk factors
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Yan Yu, Wenqiu Xu, Sufen Zhang, Suihua Feng, Feng Feng, Junshang Dai, Xiao Zhang, Peirun Tian, Shunyao Wang, Zhiguang Zhao, Wenrui Zhao, Liping Guan, Zhixu Qiu, Jianguo Zhang, Huanhuan Peng, Jiawei Lin, Qun Zhang, Weiping Chen, Huahua Li, Qiang Zhao, Gefei Xiao, Zhongzhe Li, Shihao Zhou, Can Peng, Zhen Xu, Jingjing Zhang, Rui Zhang, Xiaohong He, Hua Li, Jia Li, Xiaohong Ruan, Lijian Zhao, and Jun He more...
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preeclampsia ,non-invasive prenatal testing ,cell-free DNA ,prediction ,in vitro fertilization ,Medicine (General) ,R5-920 - Abstract
BackgroundPreeclampsia (PE) is a pregnancy complication defined by new onset hypertension and proteinuria or other maternal organ damage after 20 weeks of gestation. Although non-invasive prenatal testing (NIPT) has been widely used to detect fetal chromosomal abnormalities during pregnancy, its performance in combination with maternal risk factors to screen for PE has not been extensively validated. Our aim was to develop and validate classifiers that predict early- or late-onset PE using the maternal plasma cell-free DNA (cfDNA) profile and clinical risk factors.MethodsWe retrospectively collected and analyzed NIPT data of 2,727 pregnant women aged 24–45 years from four hospitals in China, which had previously been used to screen for fetal aneuploidy at 12 + 0 ~ 22 + 6 weeks of gestation. According to the diagnostic criteria for PE and the time of diagnosis (34 weeks of gestation), a total of 143 early-, 580 late-onset PE samples and 2,004 healthy controls were included. The wilcoxon rank sum test was used to identify the cfDNA profile for PE prediction. The Fisher’s exact test and Mann–Whitney U-test were used to compare categorical and continuous variables of clinical risk factors between PE samples and healthy controls, respectively. Machine learning methods were performed to develop and validate PE classifiers based on the cfDNA profile and clinical risk factors.ResultsBy using NIPT data to analyze cfDNA coverages in promoter regions, we found the cfDNA profile, which was differential cfDNA coverages in gene promoter regions between PE and healthy controls, could be used to predict early- and late-onset PE. Maternal age, body mass index, parity, past medical histories and method of conception were significantly differential between PE and healthy pregnant women. With a false positive rate of 10%, the classifiers based on the combination of the cfDNA profile and clinical risk factors predicted early- and late-onset PE in four datasets with an average accuracy of 89 and 80% and an average sensitivity of 63 and 48%, respectively.ConclusionIncorporating cfDNA profiles in classifiers might reduce performance variations in PE models based only on clinical risk factors, potentially expanding the application of NIPT in PE screening in the future. more...
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- 2024
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5. DREB transcription factors are crucial regulators of abiotic stress responses in Gossypium spp.
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Salisu Bello Sadau, Zhixin Liu, Vincent Ninkuu, Liping Guan, and Xuwu Sun
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Abiotic stress ,Crop improvement ,Dehydration-responsive element binding protein ,Cotton ,Plant transformation ,Transcription factors ,Plant ecology ,QK900-989 - Abstract
Plants are adversely affected by abiotic stress conditions such as heat, drought, cold, and salinity. The physiological determinants of cotton stress tolerance and its processes were investigated to mitigate the effect of abiotic stress on cotton growth and development. The DEHYDRATION-RESPONSIVE ELEMENT BINDING protein (DREB) transcription factors are stress-responsive and regulate the expression of downstream stress-inducible genes, leading to subsequent resistance to several abiotic stressors. Advanced omics technologies have recently improved our understanding of the complex molecular events of cotton that precede abiotic stress responses. We highlight the central abiotic stress resistance mechanisms in cotton, elaborating on the identified and unidentified regulatory variables. The evolutionary relationship between DREB transcription factors (TFs), divided into six subtypes in Gossypium hirsutum, Gossypium raimondii, and Arabidopsis thaliana, was reviewed. Moreover, the evolutionary antecedents, conserved motifs, and gene structure of the cotton DREB gene family have been discussed. This review offers an in-depth perspective on the contribution of DREB TFs to cotton tolerance to abiotic stress conditions, such as cold, drought, salt, heat, and heavy metals. Overall, this review identified essential genes in Gossypium spp and other species that could hasten applied studies toward their engineering into plants to mitigate abiotic stress. more...
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- 2024
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6. Comprehensive study of volatile compounds and transcriptome data providing genes for grape aroma
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Yongzhou Li, Liangliang He, Yinhua Song, Peng Zhang, Doudou Chen, Liping Guan, and Sanjun Liu
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Aroma ,Grapes ,SPME-GC-MS ,Transcriptome ,Volatile compounds ,Botany ,QK1-989 - Abstract
Abstract Background Fruit aroma is an important quality with respect to consumer preference, but the most important aroma compounds and their genetic regulatory mechanisms remain elusive. Results In this study, we qualitatively analysed volatile compounds in the pulp and skin of five table grape cultivars with three aroma types (muscat, strawberry, and neutral) using solid-phase microextraction gas chromatography/mass spectrometry. We identified 215 aroma compounds, including 88 esters, 64 terpenes, and 29 alcohols, and found significant differences in the number of compounds between the pulp and skin, especially for terpenes. Skin transcriptome data for the five grape cultivars were generated and subjected to aroma compound-gene correlation analysis. The combined transcriptomic analysis and terpene profiling data revealed 20 candidate genes, which were assessed in terms of their involvement in aroma biosynthetic regulation, including 1 VvCYP (VIT_08s0007g07730), 2 VvCCR (VIT_13s0067g00620, VIT_13s0047g00940), 3 VvADH (VIT_00s0615g00010, VIT_00s0615g00030, VIT_ 00s0615g00020), and 1 VvSDR (VIT_08s0040g01200) in the phenylpropanoids synthesis pathway, and 1 VvDXS (VIT_05s0020g02130) and 6 VvTPS (VIT_13s0067g00370, Vitis_vinifera_newGene_3216, VIT_13s0067g00380, VIT_13s0084g00010, VIT_00s0271g00010, and VIT_13s0067g00050) in the methylerythritol phosphate pathway (involved in the production and accumulation of aromatic compounds). Additionally, 2 VvMYB (VIT_17s0000g07950, VIT_03s0063g02620) and 1 VvGATA (VIT_15s0024g00980) transcription factor played important regulatory roles in the accumulation of key biosynthetic precursors of these compounds in grapes. Our results indicated that downstream genes, specifically 1 VvBGLU (VIT_03s0063g02490) and 2 VvUGT (VIT_17s0000g07070, VIT_17s0000g07060) are involved in regulating the formation and volatilization of bound compounds in grapes. Conclusions The results of this study shed light on the volatile compounds and “anchor points” of synthetic pathways in the pulp and skin of muscat and strawberry grapes, and provide new insight into the regulation of different aromas in grapes. more...
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- 2023
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7. Regulation of the tumor immune microenvironment by cancer-derived circular RNAs
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Liping Guan, Qian Hao, Fenfen Shi, Bo Gao, Mengxin Wang, Xiang Zhou, Tao Han, and Wenjie Ren
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Cytology ,QH573-671 - Abstract
Abstract Circular RNA (circRNAs) is a covalently closed circular non-coding RNA formed by reverse back-splicing from precursor messenger RNA. It is found widely in eukaryotic cells and can be released to the surrounding environment and captured by other cell types. This, circRNAs serve as connections between different cell types for the mediation of multiple signaling pathways. CircRNAs reshape the tumor microenvironment (TME), a key factor involved in all stages of cancer development, by regulating epithelial-stromal transformation, tumor vascularization, immune cell function, and inflammatory responses. Immune cells are the most abundant cellular TME components, and they have profound toxicity to cancer cells. This review summarizes circRNA regulation of immune cells, including T cells, natural killer cells, and macrophages; highlights the impact of circRNAs on tumor progression, treatment, and prognosis; and indicates new targets for tumor immunotherapy. more...
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- 2023
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8. Recent progress of the genetics of amyotrophic lateral sclerosis and challenges of gene therapy
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Hui Wang, LiPing Guan, and Min Deng
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amyotrophic lateral sclerosis ,genetics ,gene therapy ,motoneuron disease ,neurogenetics ,antisense oligonucleotide ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord. The causes of ALS are not fully understood. About 10% of ALS cases were associated with genetic factors. Since the discovery of the first familial ALS pathogenic gene SOD1 in 1993 and with the technology advancement, now over 40 ALS genes have been found. Recent studies have identified ALS related genes including ANXA11, ARPP21, CAV1, C21ORF2, CCNF, DNAJC7, GLT8D1, KIF5A, NEK1, SPTLC1, TIA1, and WDR7. These genetic discoveries contribute to a better understanding of ALS and show the potential to aid the development of better ALS treatments. Besides, several genes appear to be associated with other neurological disorders, such as CCNF and ANXA11 linked to FTD. With the deepening understanding of the classic ALS genes, rapid progress has been made in gene therapies. In this review, we summarize the latest progress on classical ALS genes and clinical trials for these gene therapies, as well as recent findings on newly discovered ALS genes. more...
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- 2023
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9. Improved cotton yield: Can we achieve this goal by regulating the coordination of source and sink?
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Aizhi Qin, Oluwaseun Olayemi Aluko, Zhixin Liu, Jincheng Yang, Mengke Hu, Liping Guan, and Xuwu Sun
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cotton ,crop yield ,coordination ,regulation mechanism ,source–sink relationship ,Plant culture ,SB1-1110 - Abstract
Cotton is one of the major cash crops globally. It is characterized by determinate growth and multiple fruiting, which makes the source–sink contradiction more obvious. Coordination between source and sink is crucial for normal growth, yield, and quality of cotton. Numerous studies reported how the assimilate transport and distribution under varying environmental cues affected crop yields. However, less is known about the functional mechanism underlying the assimilate transport between source and sink, and how their distribution impacts cotton growth. Here, we provided an overview of the assimilate transport and distribution mechanisms , and discussed the regulatory mechanisms involved in source-sink balance in relation to cotton yield. Therefore, this review enriched our knowledge of the regulatory mechanism involved in source–sink relationship for improved cotton yield. more...
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- 2023
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10. Integrative genetic and single cell RNA sequencing analysis provides new clues to the amyotrophic lateral sclerosis neurodegeneration
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Hankui Liu, Liping Guan, Min Deng, Lars Bolund, Karsten Kristiansen, Jianguo Zhang, Yonglun Luo, and Zhanchi Zhang
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amyotrophic lateral sclerosis (ALS) ,motor neurons (MNs) ,single-cell transcriptome ,genome-wide association studies (GWAS) ,neurological disorder ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
IntroductionThe gradual loss of motor neurons (MNs) in the brain and spinal cord is a hallmark of amyotrophic lateral sclerosis (ALS), but the mechanisms underlying neurodegeneration in ALS are still not fully understood.MethodsBased on 75 ALS-pathogenicity/susceptibility genes and large-scale single-cell transcriptomes of human/mouse brain/spinal cord/muscle tissues, we performed an expression enrichment analysis to identify cells involved in ALS pathogenesis. Subsequently, we created a strictness measure to estimate the dosage requirement of ALS-related genes in linked cell types.ResultsRemarkably, expression enrichment analysis showed that α- and γ-MNs, respectively, are associated with ALS-susceptibility genes and ALS-pathogenicity genes, revealing differences in biological processes between sporadic and familial ALS. In MNs, ALS-susceptibility genes exhibited high strictness, as well as the ALS-pathogenicity genes with known loss of function mechanism, indicating the main characteristic of ALS-susceptibility genes is dosage-sensitive and the loss of function mechanism of these genes may involve in sporadic ALS. In contrast, ALS-pathogenicity genes with gain of function mechanism exhibited low strictness. The significant difference of strictness between loss of function genes and gain of function genes provided a priori understanding for the pathogenesis of novel genes without an animal model. Besides MNs, we observed no statistical evidence for an association between muscle cells and ALS-related genes. This result may provide insight into the etiology that ALS is not within the domain of neuromuscular diseases. Moreover, we showed several cell types linked to other neurological diseases [i.e., spinocerebellar ataxia (SA), hereditary motor neuropathies (HMN)] and neuromuscular diseases [i.e. hereditary spastic paraplegia (SPG), spinal muscular atrophy (SMA)], including an association between Purkinje cells in brain and SA, an association between α-MNs in spinal cord and SA, an association between smooth muscle cells and SA, an association between oligodendrocyte and HMN, a suggestive association between γ-MNs and HMN, a suggestive association between mature skeletal muscle and HMN, an association between oligodendrocyte in brain and SPG, and no statistical evidence for an association between cell type and SMA.DiscussionThese cellular similarities and differences deepened our understanding of the heterogeneous cellular basis of ALS, SA, HMN, SPG, and SMA. more...
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- 2023
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11. A broken network of susceptibility genes in the monocytes of Crohn's disease patients.
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Hankui Liu, Liping Guan, Xi Su, Lijian Zhao, Qing Shu, and Jianguo Zhang
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- 2024
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12. Genome-wide association study identifies new loci associated with risk of HBV infection and disease progression
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Zheng Zeng, Hankui Liu, Huifang Xu, Haiying Lu, Yanyan Yu, Xiaoyuan Xu, Min Yu, Tao Zhang, Xiulan Tian, Hongli Xi, Liping Guan, Jianguo Zhang, Stephen J. O’Brien, and the HBVstudy consortium
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HBV infection ,Disease progression ,GWAS ,Host genetic factors ,SNPs ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Recent studies have identified susceptibility genes of HBV clearance, chronic hepatitis B, liver cirrhosis, hepatocellular carcinoma, and showed the host genetic factors play an important role in these HBV-related outcomes. Methods Collected samples from different outcomes of HBV infection and performed genotyping by Affymetrix 500 k SNP Array. GCTA tool, PLINK, and Bonferroni method were applied for analysis of genotyping and disease progression. ANOVA was used to evaluate the significance of the association between biomarkers and genotypes in healthy controls. PoMo, FST, Vcftools and Rehh package were used for building the racial tree and population analysis. FST statistics accesses 0.15 was used as a threshold to detect the signature of selection. Results There are 1031 participants passed quality control from 1104 participants, including 275 HBV clearance, 92 asymptomatic persistence infection (ASPI), 93 chronic hepatitis B (CHB), 188 HBV-related decompensated cirrhosis (DC), 214 HBV-related hepatocellular carcinoma (HCC) and 169 healthy controls (HC). In the case–control study, one novel locus significantly associated with CHB (SNP: rs1264473, Gene: GRHL2, P = 1.57 × 10−6) and HCC (SNP: rs2833856, Gene: EVA1C, P = 1.62 × 10−6; SNP: rs4661093, Gene: ETV3, P = 2.26 × 10−6). In the trend study across progressive stages post HBV infection, one novel locus (SNP: rs1537862, Gene: LACE1, P = 1.85 × 10−6), and three MHC loci (HLA-DRB1, HLA-DPB1, HLA-DPA2) showed significant increased progressive risk from ASPI to CHB. Underlying the evolutionary study of HBV-related genes in public database, the derived allele of two HBV clearance related loci, rs3077 and rs9277542, are under strong selection in European population. Conclusions In this study, we identified several novel candidate genes associated with individual HBV infectious outcomes, progressive stages, and liver enzymes. Two SNPs that show selective significance (HLA-DPA1, HLA-DPB1) in non-East Asian (European, American, South Asian) versus East Asian, indicating that host genetic factors contribute to the ethnic disparities of susceptibility of HBV infection. Taken together, these findings provided a new insight into the role of host genetic factors in HBV related outcomes and progression. more...
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- 2021
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13. Comprehensive Analysis of Role of Cyclin-Dependent Kinases Family Members in Colorectal Cancer
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Liping Guan, Yuanyuan Tang, Guanghua Li, Zhao Qin, and Shaoshan Li
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colorectal cancer ,CDK ,biomarkers ,infiltration of immune cells ,therapeutic targets ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundCyclin-dependent kinases (CDKs) are cell cycle regulators, and abnormal activation can accelerate tumor cell proliferation. However, The relation between CDKs dysregulation to colorectal cancer incidence and progression have not been examined in detail. Methods:Differences in CDKs expression between colorectal cancer and normal tissues, associations between expression and clinical prognosis, incidence and frequencies of CDKs gene mutations, and the influences of CDKs on tumor infiltration by immune cells were examined by analyses of Oncomine, Gene Expression Profiling Interactive Analysis, Kaplan-Meier plotter, cBioPortal, GeneMANIA, and TIMER databases.ResultsColorectal cancer tissues showed enhanced expression levels of CDKs 1/2/4/5/6/8/12/13/19 but reduced CDK3 expression. CDK7 was highly expressed in some colorectal cancer tissues but downregulated in others. Expression levels of CDK1/3/4/7/8/10/11b/13/18/19/20 were correlated with clinical stage, and CDK 5/10/12/16 expression levels predicted prognosis and survival. Differential CDKs expression correlated with cell cycle progression, amino acid polypeptide modifications, and activation of other protein kinases. Expression levels of all CDKs except CDK16 were correlated with infiltration of CD4+T, CD8+T, B and Tregs cells.ConclusionsCDK 1 and 4 could be used as diagnostic biomarkers for CRC. CDK 5/10/12/16 can be utilized as prognostic biomarkers. more...
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- 2022
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14. An integrated peach genome structural variation map uncovers genes associated with fruit traits
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Jian Guo, Ke Cao, Cecilia Deng, Yong Li, Gengrui Zhu, Weichao Fang, Changwen Chen, Xinwei Wang, Jinlong Wu, Liping Guan, Shan Wu, Wenwu Guo, Jia-Long Yao, Zhangjun Fei, and Lirong Wang
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SVs ,GWAS ,Peach ,Fruit ,Fruit shape ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
Abstract Background Genome structural variations (SVs) have been associated with key traits in a wide range of agronomically important species; however, SV profiles of peach and their functional impacts remain largely unexplored. Results Here, we present an integrated map of 202,273 SVs from 336 peach genomes. A substantial number of SVs have been selected during peach domestication and improvement, which together affect 2268 genes. Genome-wide association studies of 26 agronomic traits using these SVs identify a number of candidate causal variants. A 9-bp insertion in Prupe.4G186800, which encodes a NAC transcription factor, is shown to be associated with early fruit maturity, and a 487-bp deletion in the promoter of PpMYB10.1 is associated with flesh color around the stone. In addition, a 1.67 Mb inversion is highly associated with fruit shape, and a gene adjacent to the inversion breakpoint, PpOFP1, regulates flat shape formation. Conclusions The integrated peach SV map and the identified candidate genes and variants represent valuable resources for future genomic research and breeding in peach. more...
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- 2020
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15. A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes
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Zhiqiang Wei, Chunjiao Dong, Liping Guan, Yafei Wang, Jianghai Huang, and Xinzhu Wen
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Osteoarthritis ,Chondrocytes ,Ligusticum wallichii ,IL-1β ,Metabolic pathways ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Osteoarthritis (OA) is a metabolic disorder and able to be relieved by traditional Chinese medicines. However, the effect of Ligusticum wallichii on OA is unknown. Methods Cytokine IL-1β and L. wallichii extracts were used to stimulate the primary mouse chondrocytes. MTT assay was used to measure the cell viability. The mRNA and protein level of each gene were test by qRT-PCR and western blotting, respectively. The rate of apoptotic cell was measured by flow cytometry. GC/MS-based metabolomics was utilized to characterize the variation of metabolome. Results Here, we found that L. wallichii attenuated the IL-1β-induced apoptosis, inflammatory response, and extracellular matrix (ECM) degradation in mouse chondrocytes. Then we used GC/MS-based metabolomics to characterize the variation of metabolomes. The established metabolic profile of mouse chondrocytes showed that the abundance of most metabolites (n = 40) altered by IL-1β stimulation could be repressed by L. wallichii treatment. Multivariate data analysis identified that cholesterol, linoleic acid, hexadecandioic acid, proline, l-valine, l-leucine, pyruvate, palmitic acid, and proline are the most key biomarkers for understanding the metabolic role of L. wallichii in IL-1β-treated chondrocytes. Further pathway analysis using these metabolites enriched fourteen metabolic pathways, which were dramatically changed in IL-1β-treated chondrocytes and capable of being reprogrammed by L. wallichii incubation. These enriched pathways were involved in carbon metabolisms, fatty acid biosynthesis, and amino acid metabolisms. Conclusions These findings provide potential clues that metabolic strategies are linked to protective mechanisms of L. wallichii treatment in IL-1β-stimulated chondrocytes and emphasize the importance of metabolic strategies against inflammatory responses in OA development. more...
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- 2020
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16. Synthesis, Characterization and Biological Evaluation of Benzothiazole–Isoquinoline Derivative
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Weihua Liu, Donghai Zhao, Zhiwen He, Yiming Hu, Yuxia Zhu, Lingjian Zhang, Lianhai Jin, Liping Guan, and Sihong Wang
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neurodegenerative disease ,antidepressant ,benzothiazole–isoquinoline derivatives ,MAO–B ,BuChE ,molecular docking ,Organic chemistry ,QD241-441 - Abstract
Currently, no suitable clinical drugs are available for patients with neurodegenerative diseases complicated by depression. Based on a fusion technique to create effective multi–target–directed ligands (MTDLs), we synthesized a series of (R)–N–(benzo[d]thiazol–2–yl)–2–(1–phenyl–3,4–dihydroisoquinolin–2(1H)–yl) acetamides with substituted benzothiazoles and (S)–1–phenyl–1,2,3,4–tetrahydroisoquinoline. All compounds were tested for their inhibitory potency against monoamine oxidase (MAO) and cholinesterase (ChE) by in vitro enzyme activity assays, and further tested for their specific inhibitory potency against monoamine oxidase B (MAO–B) and butyrylcholinesterase (BuChE). Among them, six compounds (4b–4d, 4f, 4g and 4i) displayed excellent activity. The classical antidepressant forced swim test (FST) was used to verify the in vitro results, revealing that six compounds reduced the immobility time significantly, especially compound 4g. The cytotoxicity of the compounds was assessed by the MTT method and Acridine Orange (AO) staining, with cell viability found to be above 90% at effective compound concentrations, and not toxic to L929 cells reversibility, kinetics and molecular docking studies were also performed using compound 4g, which showed the highest MAO–B and BuChE inhibitory activities. The results of these studies showed that compound 4g binds to the primary interaction sites of both enzymes and has good blood–brain barrier (BBB) penetration. This study provides new strategies for future research on neurodegenerative diseases complicated by depression. more...
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- 2022
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17. Long Non-Coding RNA GRIK1-AS1 Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating the miR-375/IFIT2 Axis
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Qi Zhou, Yuan Li, Lujun Chen, Xiao Zheng, Tianwei Jiang, Juan Liu, Yuanyuan Fu, Liping Guan, Jingfang Ju, and Changping Wu
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long non-coding RNA ,lncRNA GRIK1-AS1 ,miR-375 ,IFIT2 ,gastric cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Long non-coding RNAs (lncRNAs) play important roles in various biological processes and human diseases, including cancer. In this study, we demonstrated a regulatory relationship between lncRNA GRIK1-AS1 and miR-375/IFIT2 axis in gastric cancer. Our results show a decreased expression of GRIK1-AS1 in gastric cancer tissues compared to adjacent normal gastric tissues. Gastric cell lines also have reduced levels of GRIK1-AS1 compared to gastric epithelial cell line GES-1. Ectopic expression of GRIK1-AS1 in gastric cancer cell lines significantly inhibits cellular viability, migration, and invasion. RNA-pull down and the luciferase activity assays show that GRIK1-AS1 mainly interacts specifically with miR-375. We further demonstrate a negatively regulatory relationship between lncRNA GRIK1-AS1 and miR-375. We discovered that IFIT2 was one of the direct key downstream target genes of miR-375, and established the important role of the GRIK1-AS1/miR-375/IFIT2 axis in the progression of gastric cancer. Taken together, our results revealed a novel mechanism of GRIK1-AS1 as a sponge to miR-375 that impacts gastric cancer progression via modulating target mRNA IFIT2 translation, and as a result, opens a new strategy to future GRIK1-AS1 based therapeutic development. more...
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- 2021
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18. Detection and application of genome-wide variations in peach for association and genetic relationship analysis
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Liping Guan, Ke Cao, Yong Li, Jian Guo, Qiang Xu, and Lirong Wang
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Genome-wide variations ,Detection ,Application ,Peach ,Genetics ,QH426-470 - Abstract
Abstract Background Peach (Prunus persica L.) is a diploid species and model plant of the Rosaceae family. In the past decade, significant progress has been made in peach genetic research via DNA markers, but the number of these markers remains limited. Results In this study, we performed a genome-wide DNA markers detection based on sequencing data of six distantly related peach accessions. A total of 650,693~1,053,547 single nucleotide polymorphisms (SNPs), 114,227~178,968 small insertion/deletions (InDels), 8386~12,298 structure variants (SVs), 2111~2581 copy number variants (CNVs) and 229,357~346,940 simple sequence repeats (SSRs) were detected and annotated. To demonstrate the application of DNA markers, 944 SNPs were filtered for association study of fruit ripening time and 15 highly polymorphic SSRs were selected to analyze the genetic relationship among 221 accessions. Conclusions The results showed that the use of high-throughput sequencing to develop DNA markers is fast and effective. Comprehensive identification of DNA markers, including SVs and SSRs, would be of benefit to genetic diversity evaluation, genetic mapping, and molecular breeding of peach. more...
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- 2019
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19. Genomic analyses of an extensive collection of wild and cultivated accessions provide new insights into peach breeding history
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Yong Li, Ke Cao, Gengrui Zhu, Weichao Fang, Changwen Chen, Xinwei Wang, Pei Zhao, Jian Guo, Tiyu Ding, Liping Guan, Qian Zhang, Wenwu Guo, Zhangjun Fei, and Lirong Wang
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Genomics ,Whole genome resequencing ,Domestication ,Improvement ,Human selection ,Breeding history ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
Abstract Background Human selection has a long history of transforming crop genomes. Peach (Prunus persica) has undergone more than 5000 years of domestication that led to remarkable changes in a series of agronomically important traits, but genetic bases underlying these changes and the effects of artificial selection on genomic diversity are not well understood. Results Here, we report a comprehensive analysis of peach evolution based on genome sequences of 480 wild and cultivated accessions. By focusing on a set of quantitative trait loci (QTLs), we provide evidence supporting that distinct phases of domestication and improvement have led to an increase in fruit size and taste and extended its geographic distribution. Fruit size was predominantly selected during domestication, and selection for large fruits has led to the loss of genetic diversity in several fruit weight QTLs. In contrast, fruit taste-related QTLs were successively selected for by domestication and improvement, with more QTLs selected for during improvement. Genome-wide association studies of 11 agronomic traits suggest a set of candidate genes controlling these traits and potential markers for molecular breeding. Candidate loci for genes that contributed to the adaption to low-chill regions were identified. Furthermore, the genomic bases of divergent selection for fruit texture and local breeding for different flavors between Asian and European/North American cultivars were also determined. Conclusions Our results elucidate the genetic basis of peach evolution and provide new resources for future genomics-guided peach breeding. more...
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- 2019
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20. Antigen-specific CD8+ memory stem T cells generated from human peripheral blood effectively eradicate allogeneic targets in mice
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Liping Guan, Xiaoyi Li, Jiali Wei, Zhihui Liang, Jing Yang, Xiufang Weng, and Xiongwen Wu
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T memory stem cells ,Allogeneic antigen specificity ,Preparation in vitro ,Adoptive immunotherapy ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background As the implantation and long-term existence of tumor-specific T cells in host are the prerequisite for adoptive immunotherapy, memory stem T cells (TSCM) with self-renewal and differentiation capacity show the greatest potential to implant and long-term exhibit function in vivo, compared with other T cells of differentiation stages. Hence, tumor-specific TSCM have become potential candidate for adoptive T cell therapy of cancer. Here, we reported a protocol to generate allogeneic antigen-specific CD8+ TSCM cells from human PBLs. Methods To prepare allogeneic antigen-specific CD8+ TSCM, we used an LCL named E007 of defined HLA allotyping as simulator, a co-culture of E007 and allogeneic PBLs was carried out in the presence of differentiation inhibitor TWS119 for 7 days. Sorting of proliferation cells ensured the E007-specificity of the prepared TSCM cells. The sorted lymphocytes underwent further expansion by cytokines IL-7 and IL-15 for further 7 days, making the E007-specific CD8 + TSCM expanded in number. The stem cell and T memory cell properties of the prepared CD8+ TSCM were observed in NOD-SCID mice. Results Our protocol began with 1 × 107 PBLs and resulted in 2 × 107 E007-specific CD8+ TSCM cells in 2 weeks of preparation. The prepared TSCM cells exhibited a proliferative history and rapid differentiation into effector cells upon the E007 re-stimulation. Importantly, the prepared TSCM cells were able to exist long and reconstitute other T cell subsets in vivo, eradicating the E007 cells effectively after transferred into the LCL burden mice. Conclusions This protocol was able to prepare allogeneic antigen-specific CD8+ TSCM cells from human PBLs. The prepared TSCM showed the properties of stem cells and T memory cells. This study provided a reference method for generation of antigen-specific TSCM for T cell adoptive immunotherapy. more...
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- 2018
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21. Revealing Alzheimer’s disease genes spectrum in the whole-genome by machine learning
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Xiaoyan Huang, Hankui Liu, Xinming Li, Liping Guan, Jiankang Li, Laurent Christian Asker M. Tellier, Huanming Yang, Jian Wang, and Jianguo Zhang
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Alzheimer’s disease ,Gene ,Machine learning ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Alzheimer’s disease (AD) is an important, progressive neurodegenerative disease, with a complex genetic architecture. A key goal of biomedical research is to seek out disease risk genes, and to elucidate the function of these risk genes in the development of disease. For this purpose, expanding the AD-associated gene set is necessary. In past research, the prediction methods for AD related genes has been limited in their exploration of the target genome regions. We here present a genome-wide method for AD candidate genes predictions. Methods We present a machine learning approach (SVM), based upon integrating gene expression data with human brain-specific gene network data, to discover the full spectrum of AD genes across the whole genome. Results We classified AD candidate genes with an accuracy and the area under the receiver operating characteristic (ROC) curve of 84.56% and 94%. Our approach provides a supplement for the spectrum of AD-associated genes extracted from more than 20,000 genes in a genome wide scale. Conclusions In this study, we have elucidated the whole-genome spectrum of AD, using a machine learning approach. Through this method, we expect for the candidate gene catalogue to provide a more comprehensive annotation of AD for researchers. more...
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- 2018
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22. A phrase-based model to discover hidden factors and hidden topics in recommender systems.
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Liping Guan, Md. Hijbul Alam, Woo-Jong Ryu, and SangKeun Lee 0001
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- 2016
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23. Comparative Transcriptome and Microscopy Analyses Provide Insights into Flat Shape Formation in Peach (Prunus persica)
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Jian Guo, Ke Cao, Yong Li, Jia-Long Yao, Cecilia Deng, Qi Wang, Gengrui Zhu, Weichao Fang, Changwen Chen, Xinwei Wang, Liping Guan, Tiyu Ding, and Lirong Wang
- Subjects
fruit shape ,peach ,transcriptome ,cell number and size ,fruit diameter ,fruit development ,Plant culture ,SB1-1110 - Abstract
Fruit shape is an important external characteristic that consumers use to select preferred fruit cultivars. In peach, the flat fruit cultivars have become more and more popular worldwide. Genetic markers closely linking to the flat fruit trait have been identified and are useful for marker-assisted breeding. However, the cellular and genetic mechanisms underpinning flat fruit formation are still poorly understood. In this study, we have revealed the differences in fruit cell number, cell size, and in gene expression pattern between the traditional round fruit and modern flat fruit cultivars. Flat peach cultivars possessed significantly lower number of cells in the vertical axis because cell division in the vertical direction stopped early in the flat fruit cultivars at 15 DAFB (day after full bloom) than in round fruit cultivars at 35 DAFB. This resulted in the reduction in vertical development in the flat fruit. Significant linear relationship was observed between fruit vertical diameter and cell number in vertical axis for the four examined peach cultivars (R2 = 0.9964) at maturation stage, and was also observed between fruit vertical diameter and fruit weight (R2 = 0.9605), which indicated that cell number in vertical direction contributed to the flat shape formation. Furthermore, in RNA-seq analysis, 4165 differentially expressed genes (DEGs) were detected by comparing RNA-seq data between flat and round peach cultivars at different fruit development stages. In contrast to previous studies, we discovered 28 candidate genes potentially responsible for the flat shape formation, including 19 located in the mapping site and 9 downstream genes. Our study indicates that flat and round fruit shape in peach is primarily determined by the regulation of cell production in the vertical direction during early fruit development. more...
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- 2018
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24. Synthesis, potential anticonvulsant and antidepressant effects of 2-(5-methyl-2,3-dioxoindolin-1-yl)acetamide derivatives
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Xinghua Zhen, Zhou Peng, Shuilian Zhao, Yan Han, Qinghao Jin, and Liping Guan
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2,3-Dioxoindolin-1-acetamide ,Synthesis ,Anticonvulsant activity ,Antidepressant activity ,Pentylenetetrazole ,Therapeutics. Pharmacology ,RM1-950 - Abstract
A new series of 2-(5-methyl-2,3-dioxoindolin-1-yl)acetamide derivatives were synthesized and evaluated for their anticonvulsive activity in a pentylenetetrazole (PTZ)-evoked convulsion model and antidepressant activity in the forced swimming test (FST) model. Eleven synthesized compounds were found to be protective against PTZ-induced seizure and showed the anticonvulsant activity. In addition, four of the synthesized compounds (4l, 4m, 4p and 4q) showed potent antidepressant-like activity. Among these compounds, compound 4l was found to have the most potent antidepressant-like activity, and significantly reduced the duration of immobility time at 100 mg/kg dose level when compared to the vehicle control, which is similar to the reference drug fluoxetine. more...
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- 2015
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25. Minimum distance clustering algorithm based on an improved differential evolution.
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Xiangyuan Yin, Zhihao Ling, Liping Guan, and Feng Liang
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- 2014
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26. Study on synthesis of naringenin derivatives and cholinesterase inhibitory activity in marine Chinese medicine.
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Yuxia Zhu, Lingjian Zhang, Yiming Hu, Weihua Liu, Liping Guan, and Lin Lin
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CHINESE medicine ,CHALCONE ,NARINGENIN ,COUMARINS ,LEAD compounds ,DRUG design ,MOLECULAR docking - Abstract
Copyright of Journal of Chinese Pharmaceutical Sciences is the property of Journal of Chinese Pharmaceutical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2023
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27. Research on Automatic Incident Detection Algorithm Based on Fusion of Freeway Mainline Information and Toll Collection information.
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Xiangyuan Yin, Weiming Liu 0003, and Liping Guan
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- 2006
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28. A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.
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Ximing Chen, Lizhe An, Xiaochuan Fan, Furong Ju, Binglin Zhang, Haili Sun, Jianxi Xiao, Wei Hu, Tao Qu, Liping Guan, Shukun Tang, Tuo Chen, Guangxiu Liu, and Paul Dyson
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Genetics ,QH426-470 - Abstract
The dissacharide trehalose is an important intracellular osmoprotectant and the OtsA/B pathway is the principal pathway for trehalose biosynthesis in a wide range of bacterial species. Scaffolding proteins and other cytoskeletal elements play an essential role in morphogenetic processes in bacteria. Here we describe how OtsA, in addition to its role in trehalose biosynthesis, functions as an osmotic stress sensor to regulate cell morphology in Arthrobacter strain A3. In response to osmotic stress, this and other Arthrobacter species undergo a transition from bacillary to myceloid growth. An otsA null mutant exhibits constitutive myceloid growth. Osmotic stress leads to a depletion of trehalose-6-phosphate, the product of the OtsA enzyme, and experimental depletion of this metabolite also leads to constitutive myceloid growth independent of OtsA function. In vitro analyses indicate that OtsA can self-assemble into protein networks, promoted by trehalose-6-phosphate, a property that is not shared by the equivalent enzyme from E. coli, despite the latter's enzymatic activity when expressed in Arthrobacter. This, and the localization of the protein in non-stressed cells at the mid-cell and poles, indicates that OtsA from Arthrobacter likely functions as a cytoskeletal element regulating cell morphology. Recruiting a biosynthetic enzyme for this morphogenetic function represents an intriguing adaptation in bacteria that can survive in extreme environments. more...
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- 2017
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29. Syntheses of Benzo[d]Thiazol-2(3H)-One Derivatives and Their Antidepressant and Anticonvulsant Effects
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Qinghao Jin, Zhiyang Fu, Liping Guan, and Haiying Jiang
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benzo[d]thiazol ,synthesis ,antarctic-derived fungus ,antidepressant ,anticonvulsant ,Biology (General) ,QH301-705.5 - Abstract
Thirty-four new benzo[d]thiazol derivatives 2a−2i, 3a−3r, and 4a−4g were synthesized and investigated for their potential antidepressant and anticonvulsant effects. In a forced swimming test, 2c and 2d showed the highest antidepressant and anticonvulsant effects. 2c and 2d displayed a higher percentage decrease in immobility duration (89.96% and 89.62%, respectively) than that of fluoxetine (83.62%). In the maximal electroshock seizure test, 3n and 3q showed the highest anticonvulsant effect, with ED50 values of 46.1 and 64.3 mg kg−1, and protective indices of 6.34 and 4.11, respectively, which were similar to those of phenobarbital or valproate. We also found that the mechanism for the antidepressant activity of 2c and 2d may be via increasing the concentrations of serotonin and norepinephrine. more...
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- 2019
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30. Genomic analyses provide insights into peach local adaptation and responses to climate change
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Wenwu Guo, Weichao Fang, Zhangjun Fei, Sanwen Huang, Changwen Chen, Pere Arús, Jiao Wang, Jian Guo, Liping Guan, Xinwei Wang, Junxiu Wang, Lirong Wang, Qi Wang, Ke Cao, Kuozhan Liu, Nan Li, Gengrui Zhu, Tiyu Ding, Yong Li, National Natural Science Foundation of China, National Science Foundation (US), Producció Vegetal, and Genòmica i Biotecnologia more...
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Prunus persica ,0303 health sciences ,Candidate gene ,Natural selection ,Human evolutionary genetics ,Research ,Climate Change ,Genomics ,Biology ,Adaptation, Physiological ,Genome ,03 medical and health sciences ,0302 clinical medicine ,Evolutionary biology ,Genetics ,Adaptation ,Genome, Plant ,030217 neurology & neurosurgery ,Genetics (clinical) ,Selection (genetic algorithm) ,030304 developmental biology ,Genetic association ,Local adaptation - Abstract
The environment has constantly shaped plant genomes, but the genetic bases underlying how plants adapt to environmental influences remain largely unknown. We constructed a high-density genomic variation map of 263 geographically representative peach landraces and wild relatives. A combination of whole-genome selection scans and genome-wide environmental association studies (GWEAS) was performed to reveal the genomic bases of peach adaptation to diverse climates. A total of 2092 selective sweeps that underlie local adaptation to both mild and extreme climates were identified, including 339 sweeps conferring genomic pattern of adaptation to high altitudes. Using genome-wide environmental association studies (GWEAS), a total of 2755 genomic loci strongly associated with 51 specific environmental variables were detected. The molecular mechanism underlying adaptive evolution of high drought, strong UVB, cold hardiness, sugar content, flesh color, and bloom date were revealed. Finally, based on 30 yr of observation, a candidate gene associated with bloom date advance, representing peach responses to global warming, was identified. Collectively, our study provides insights into molecular bases of how environments have shaped peach genomes by natural selection and adds candidate genes for future studies on evolutionary genetics, adaptation to climate changes, and breeding., This work was supported by grants from the Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2020-ZFRI01), the National Natural Science Foundation of China (31572094), the Crop Germplasm Resources Conservation Project (2016NWB041), and the U.S. National Science Foundation (IOS-1339287 and IOS-1855585). more...
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- 2021
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31. A systematic analysis in efficacy and safety of nimotuzumab combined with chemoradiotherapy in treatment of advanced nasopharyngeal carcinoma
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Yunqin Sun, Yaofeng Wang, and Liping Guan
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Otorhinolaryngology ,General Medicine - Abstract
To analyze the clinical effect of nitorzumab injection combined with chemoradiotherapy in the treatment of advanced nasopharyngeal carcinoma.The databases, such as CNKI, Wanfang, VIP, China Biology Medicine (CBM), PubMed, Cochrane Library, Wiley Online Library, and Google Academic were searched. The randomized controlled trials (RCT) of nimotuzumab combined with concurrent chemoradiotherapy (experimental group) and concurrent chemoradiotherapy (control group) were searched. The between-group differences of objective remission rate (ORR), disease control rate (DCR), and drug-related adverse reactions were analyzed by RevMan5.3 software.Totally, 11 studies were included in meta-analysis, including 655 patients. All 11 articles mentioned random grouping and no blind method was used. The objective remission rate, disease control rate, and adverse drug reactions are given in 11 articles. In this study, 11 literatures were analyzed by fixed effect model after heterogeneity and sensitivity analysis. The meta analysis showed that in 10 literatures, the objective remission rate and disease control rate of patients in the experimental group were significantly higher than those in the control group (RR = 1.32, 95% CI: 1.2-1.45, Z = 5.72, P 0.00001); (RR = 1.07, 95% CI: 1.02-1.11, Z = 3.04, P = 0.002 0.01. There was no significant difference in adverse reactions between the two groups (RR = 0.95, 95% CI: 0.79-1.15, Z = 0.52, P = 0.6 0.05).The efficacy and safety of nituozumab injection combined with concurrent chemoradiotherapy are reliable and definite. more...
- Published
- 2022
32. Exome Sequencing of a Pedigree Reveals S339L Mutation in the TLN2 Gene as a Cause of Fifth Finger Camptodactyly.
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Hao Deng, Sheng Deng, Hongbo Xu, Han-Xiang Deng, Yulan Chen, Lamei Yuan, Xiong Deng, Shengbo Yang, Liping Guan, Jianguo Zhang, Hong Yuan, and Yi Guo
- Subjects
Medicine ,Science - Abstract
Camptodactyly is a digit deformity characterized by permanent flexion contracture of one or both fifth fingers at the proximal interphalangeal joints. Though over 60 distinct types of syndromic camptodactyly have been described, only one disease locus (3q11.2-q13.12) for nonsyndromic camptodactyly has been identified. To identify the genetic defect for camptodactyly in a four-generation Chinese Han family, exome and Sanger sequencings were conducted and a missense variant, c.1016C>T (p.S339L), in the talin 2 gene (TLN2) was identified. The variant co-segregated with disease in the family and was not observed in 12 unaffected family members or 1,000 normal controls, suggesting that p.S339L is a pathogenic mutation. Two asymptomatic carriers in the family indicated incomplete penetrance or more complicated compensated mechanism. Most of p.S339L carriers also have relatively benign cardiac phenotypes. Expression of wild and mutant TLN2 in HEK293 cells suggested the predominant localization in cytoplasm. Our data suggest a potential molecular link between TLN2 and camptodactyly pathogenesis. more...
- Published
- 2016
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33. Identification of a Novel Missense FBN2 Mutation in a Chinese Family with Congenital Contractural Arachnodactyly Using Exome Sequencing.
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Hao Deng, Qian Lu, Hongbo Xu, Xiong Deng, Lamei Yuan, Zhijian Yang, Yi Guo, Qiongfen Lin, Jingjing Xiao, Liping Guan, and Zhi Song
- Subjects
Medicine ,Science - Abstract
Congenital contractural arachnodactyly (CCA, OMIM 121050), also known as Beals-Hecht syndrome, is an autosomal dominant disorder of connective tissue. CCA is characterized by arachnodactyly, dolichostenomelia, pectus deformities, kyphoscoliosis, congenital contractures and a crumpled appearance of the helix of the ear. The aim of this study is to identify the genetic cause of a 4-generation Chinese family of Tujia ethnicity with congenital contractural arachnodactyly by exome sequencing. The clinical features of patients in this family are consistent with CCA. A novel missense mutation, c.3769T>C (p.C1257R), in the fibrillin 2 gene (FBN2) was identified responsible for the genetic cause of our family with CCA. The p.C1257R mutation occurs in the 19th calcium-binding epidermal growth factor-like (cbEGF) domain. The amino acid residue cysteine in this domain is conserved among different species. Our findings suggest that exome sequencing is a powerful tool to discover mutation(s) in CCA. Our results may also provide new insights into the cause and diagnosis of CCA, and may have implications for genetic counseling and clinical management. more...
- Published
- 2016
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34. A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes
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Xinzhu Wen, Yafei Wang, Liping Guan, Chunjiao Dong, Zhiqiang Wei, and Jianghai Huang
- Subjects
Linoleic acid ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Metabolomics ,Chondrocytes ,Osteoarthritis ,medicine ,Metabolome ,MTT assay ,Viability assay ,Ligusticum wallichii ,030304 developmental biology ,030203 arthritis & rheumatology ,Pharmacology ,0303 health sciences ,Ligusticum ,biology ,Chemistry ,Research ,Metabolic disorder ,lcsh:Other systems of medicine ,medicine.disease ,biology.organism_classification ,lcsh:RZ201-999 ,Metabolic pathway ,Complementary and alternative medicine ,Biochemistry ,IL-1β ,Metabolic pathways - Abstract
Background Osteoarthritis (OA) is a metabolic disorder and able to be relieved by traditional Chinese medicines. However, the effect of Ligusticum wallichii on OA is unknown. Methods Cytokine IL-1β and L. wallichii extracts were used to stimulate the primary mouse chondrocytes. MTT assay was used to measure the cell viability. The mRNA and protein level of each gene were test by qRT-PCR and western blotting, respectively. The rate of apoptotic cell was measured by flow cytometry. GC/MS-based metabolomics was utilized to characterize the variation of metabolome. Results Here, we found that L. wallichii attenuated the IL-1β-induced apoptosis, inflammatory response, and extracellular matrix (ECM) degradation in mouse chondrocytes. Then we used GC/MS-based metabolomics to characterize the variation of metabolomes. The established metabolic profile of mouse chondrocytes showed that the abundance of most metabolites (n = 40) altered by IL-1β stimulation could be repressed by L. wallichii treatment. Multivariate data analysis identified that cholesterol, linoleic acid, hexadecandioic acid, proline, l-valine, l-leucine, pyruvate, palmitic acid, and proline are the most key biomarkers for understanding the metabolic role of L. wallichii in IL-1β-treated chondrocytes. Further pathway analysis using these metabolites enriched fourteen metabolic pathways, which were dramatically changed in IL-1β-treated chondrocytes and capable of being reprogrammed by L. wallichii incubation. These enriched pathways were involved in carbon metabolisms, fatty acid biosynthesis, and amino acid metabolisms. Conclusions These findings provide potential clues that metabolic strategies are linked to protective mechanisms of L. wallichii treatment in IL-1β-stimulated chondrocytes and emphasize the importance of metabolic strategies against inflammatory responses in OA development. more...
- Published
- 2020
35. Synthesis and Biological Activity Evaluation of Benzothiazole-Isoquinoline Derivatives
- Author
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Wei-Hua Liu, liping guan, Dong-Hai Zhao, Zhi-Wen He, Yi-Ming Hu, Yu-Xia Zhu, Ling-Jian Zhang, Lian-Hai Jin, Li-Ping Guan, and Si-Hong Wang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
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36. Large-Scale Screening of Thalassemia in Ji’an, P.R. China
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Yu Qiu, Liangwei Mao, Shiping Chen, Hao Li, Hairong Wang, Liping Guan, Jin Huang, Xuan Wu, Yu Liu, Jie Xiao, and Yuan Fang
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congenital, hereditary, and neonatal diseases and abnormalities ,hemic and lymphatic diseases - Abstract
Background: To evaluate the prevalence of alpha- and beta-thalassemia in Ji'an City, Jiangxi Province, 28,941 people in the region were genetically screened to identify various thalassemia genotypes. Methods: High-throughput amplicon sequencing and gap-PCR was used to screen 301 thalassemia alleles in 28,941 people in the region. Pregnant women were the focus of this screening, and if a pregnant woman harbored mutations in a thalassemia-inducing gene, her spouse was also genetically tested. Results: Of the participants, 2,380 people were carriers of thalassemia, with at least one thalassemia allele, including 1,742 alpha-thalassemia carriers, 686 beta-thalassemia carriers and 48 composite alpha- and beta-thalassemia carriers. The total carrying rate of thalassemia in Ji'an was 8.22%, and the carrying rates of alpha- and beta-thalassemia were 6% and 2.37%, respectively. In addition, the first measured carrier rate of composite alpha- and beta-thalassemia in Ji'an was 0.17%. According to the geographical distribution of the 1,742 alpha -thalassemia carriers, the city with the highest carrier rate was Suichuan, followed by Wan’an and Taihe. According to the geographical distribution of the 686 beta-thalassemia carriers, the top three cities with high carrier rates were Suichuan, Wan'an and Xiajiang, sequentially. Conclusions: This research emphasizes the importance of large-scale population screening and that comprehensive molecular epidemiology data are necessary for the proper prevention and treatment of thalassemia. The epidemiological data updated in this research may enable the local government to focus on the severity of this disease and determine a method for effective resource allocation under limited resource conditions. more...
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- 2022
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37. Identification of Novel Sphydrofuran-Derived Derivatives with Lipid-Lowering Activity from the Active Crude Extracts of Nocardiopsis sp. ZHD001
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Yuhong Tian, Yongjun Jiang, Zhengshun Wen, Liping Guan, Xiaokun Ouyang, Wanjing Ding, and Zhongjun Ma
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Inorganic Chemistry ,Nocardiopsis sp ,structure elucidation ,Organic Chemistry ,General Medicine ,sphydrofuran derivatives ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,lipid-lowering ,Computer Science Applications - Abstract
Lipid-lowering is one of the most effective methods of prevention and treatment for cardiovascular diseases. However, most clinical lipid-lowering drugs have adverse effects and cannot achieve the desired efficacy in some complex hyperlipidemia patients, so it is of great significance to develop safe and effective novel lipid-lowering drugs. In the course of our project aimed at discovering the chemical novelty and bioactive natural products of marine-derived actinomycetes, we found that the organic crude extracts (OCEs) of Nocardiopsis sp. ZHD001 exhibited strong in vivo efficacies in reducing weight gain, lowering LDL-C, TC, and TG levels, and improving HDL-C levels in high-fat-diet-fed mice models. Chemical investigations of the active OCEs led to identifying two new sphydrofuran-derived compounds (1–2) and one known 2-methyl-4-(1-glycerol)-furan (3). Their structures were elucidated by the analysis of HRESIMS, 1D and 2D NMR spectroscopic data, and ECD calculations. Among these compounds, compound 1 represents a novel rearranged sphydrofuran-derived derivative. Bioactivity evaluations of these pure compounds showed that all the compounds exhibited significant lipid-lowering activity with lower cytotoxicity in vitro compared to simvastatin. Our results demonstrate that sphydrofuran-derived derivatives might be promising candidates for lipid-lowering drugs. more...
- Published
- 2023
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38. Application of Whole Exome Sequencing in Six Families with an Initial Diagnosis of Autosomal Dominant Retinitis Pigmentosa: Lessons Learned.
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Berta Almoguera, Jiankang Li, Patricia Fernandez-San Jose, Yichuan Liu, Michael March, Renata Pellegrino, Ryan Golhar, Marta Corton, Fiona Blanco-Kelly, Maria Isabel López-Molina, Blanca García-Sandoval, Yiran Guo, Lifeng Tian, Xuanzhu Liu, Liping Guan, Jianguo Zhang, Brendan Keating, Xun Xu, Hakon Hakonarson, and Carmen Ayuso more...
- Subjects
Medicine ,Science - Abstract
This study aimed to identify the genetics underlying dominant forms of inherited retinal dystrophies using whole exome sequencing (WES) in six families extensively screened for known mutations or genes. Thirty-eight individuals were subjected to WES. Causative variants were searched among single nucleotide variants (SNVs) and insertion/deletion variants (indels) and whenever no potential candidate emerged, copy number variant (CNV) analysis was performed. Variants or regions harboring a candidate variant were prioritized and segregation of the variant with the disease was further assessed using Sanger sequencing in case of SNVs and indels, and quantitative PCR (qPCR) for CNVs. SNV and indel analysis led to the identification of a previously reported mutation in PRPH2. Two additional mutations linked to different forms of retinal dystrophies were identified in two families: a known frameshift deletion in RPGR, a gene responsible for X-linked retinitis pigmentosa and p.Ser163Arg in C1QTNF5 associated with Late-Onset Retinal Degeneration. A novel heterozygous deletion spanning the entire region of PRPF31 was also identified in the affected members of a fourth family, which was confirmed with qPCR. This study allowed the identification of the genetic cause of the retinal dystrophy and the establishment of a correct diagnosis in four families, including a large heterozygous deletion in PRPF31, typically considered one of the pitfalls of this method. Since all findings in this study are restricted to known genes, we propose that targeted sequencing using gene-panel is an optimal first approach for the genetic screening and that once known genetic causes are ruled out, WES might be used to uncover new genes involved in inherited retinal dystrophies. more...
- Published
- 2015
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39. Detection and application of genome-wide variations in peach for association and genetic relationship analysis
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Jian Guo, Lirong Wang, Qiang Xu, Yong Li, Ke Cao, and Liping Guan
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0106 biological sciences ,0301 basic medicine ,Genetic Markers ,DNA Copy Number Variations ,lcsh:QH426-470 ,Application ,Single-nucleotide polymorphism ,Biology ,01 natural sciences ,Genome ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Gene mapping ,INDEL Mutation ,Genetic variation ,Genetics ,Copy-number variation ,Genome-wide variations ,Genetics (clinical) ,Prunus persica ,Genetic diversity ,Whole Genome Sequencing ,Chromosome Mapping ,High-Throughput Nucleotide Sequencing ,food and beverages ,Peach ,Detection ,lcsh:Genetics ,030104 developmental biology ,Genetic marker ,Microsatellite ,010606 plant biology & botany ,Research Article ,Microsatellite Repeats - Abstract
Background Peach (Prunus persica L.) is a diploid species and model plant of the Rosaceae family. In the past decade, significant progress has been made in peach genetic research via DNA markers, but the number of these markers remains limited. Results In this study, we performed a genome-wide DNA markers detection based on sequencing data of six distantly related peach accessions. A total of 650,693~1,053,547 single nucleotide polymorphisms (SNPs), 114,227~178,968 small insertion/deletions (InDels), 8386~12,298 structure variants (SVs), 2111~2581 copy number variants (CNVs) and 229,357~346,940 simple sequence repeats (SSRs) were detected and annotated. To demonstrate the application of DNA markers, 944 SNPs were filtered for association study of fruit ripening time and 15 highly polymorphic SSRs were selected to analyze the genetic relationship among 221 accessions. Conclusions The results showed that the use of high-throughput sequencing to develop DNA markers is fast and effective. Comprehensive identification of DNA markers, including SVs and SSRs, would be of benefit to genetic diversity evaluation, genetic mapping, and molecular breeding of peach. more...
- Published
- 2019
40. Genomic analyses of an extensive collection of wild and cultivated accessions provide new insights into peach breeding history
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Xinwei Wang, Liping Guan, Wenwu Guo, Weichao Fang, Zhangjun Fei, Pei Zhao, Zhang Qian, Ke Cao, Lirong Wang, Yong Li, Changwen Chen, Gengrui Zhu, Jian Guo, and Tiyu Ding
- Subjects
Candidate gene ,lcsh:QH426-470 ,Quantitative Trait Loci ,Genomics ,Quantitative trait locus ,Biology ,Breeding history ,Genome ,Domestication ,03 medical and health sciences ,0302 clinical medicine ,Improvement ,Human selection ,Selection, Genetic ,lcsh:QH301-705.5 ,Selection (genetic algorithm) ,030304 developmental biology ,2. Zero hunger ,Molecular breeding ,Prunus persica ,0303 health sciences ,Genetic diversity ,Research ,food and beverages ,Cold Temperature ,Plant Breeding ,lcsh:Genetics ,Whole genome resequencing ,lcsh:Biology (General) ,Evolutionary biology ,Fruit ,030217 neurology & neurosurgery ,Genome, Plant - Abstract
Background Human selection has a long history of transforming crop genomes. Peach (Prunus persica) has undergone more than 5000 years of domestication that led to remarkable changes in a series of agronomically important traits, but genetic bases underlying these changes and the effects of artificial selection on genomic diversity are not well understood. Results Here, we report a comprehensive analysis of peach evolution based on genome sequences of 480 wild and cultivated accessions. By focusing on a set of quantitative trait loci (QTLs), we provide evidence supporting that distinct phases of domestication and improvement have led to an increase in fruit size and taste and extended its geographic distribution. Fruit size was predominantly selected during domestication, and selection for large fruits has led to the loss of genetic diversity in several fruit weight QTLs. In contrast, fruit taste-related QTLs were successively selected for by domestication and improvement, with more QTLs selected for during improvement. Genome-wide association studies of 11 agronomic traits suggest a set of candidate genes controlling these traits and potential markers for molecular breeding. Candidate loci for genes that contributed to the adaption to low-chill regions were identified. Furthermore, the genomic bases of divergent selection for fruit texture and local breeding for different flavors between Asian and European/North American cultivars were also determined. Conclusions Our results elucidate the genetic basis of peach evolution and provide new resources for future genomics-guided peach breeding. Electronic supplementary material The online version of this article (10.1186/s13059-019-1648-9) contains supplementary material, which is available to authorized users. more...
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- 2019
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41. A novel DFNA36 mutation in TMC1 orthologous to the Beethoven (Bth) mouse associated with autosomal dominant hearing loss in a Chinese family.
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Yali Zhao, Dayong Wang, Liang Zong, Feifan Zhao, Liping Guan, Peng Zhang, Wei Shi, Lan Lan, Hongyang Wang, Qian Li, Bing Han, Ling Yang, Xin Jin, Jian Wang, Jun Wang, and Qiuju Wang
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Medicine ,Science - Abstract
Mutations in the transmembrane channel-like gene 1 (TMC1) can cause both DFNA36 and DFNB7/11 hearing loss. More than thirty DFNB7/11 mutations have been reported, but only three DFNA36 mutations were reported previously. In this study, we found a large Chinese family with 222 family members showing post-lingual, progressive sensorineural hearing loss which were consistent with DFNA36 hearing loss. Auditory brainstem response (ABR) test of the youngest patient showed a special result with nearly normal threshold but prolonged latency, decreased amplitude, and the abnormal waveform morphology. Exome sequencing of the proband found four candidate variants in known hearing loss genes. Sanger sequencing in all family members found a novel variant c.1253T>A (p.M418K) in TMC1 at DFNA36 that co-segregated with the phenotype. This mutation in TMC1 is orthologous to the mutation found in the hearing loss mouse model named Bth ten years ago. In another 51 Chinese autosomal dominant hearing loss families, we screened the segments containing the dominant mutations of TMC1 and no functional variants were found. TMC1 is expressed in the hair cells in inner ear. Given the already known roles of TMC1 in the mechanotransduction in the cochlea and its expression in inner ear, our results may provide an interesting perspective into its function in inner ear. more...
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- 2014
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42. 浙江地区新生儿耳聋基因多中心深度筛查研究
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Liping Guan
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Multiisolate ,Medical ,Phenotype or Genotype - Abstract
本项目对浙江地区多中心(东西南北地区及舟山群岛的10个主要医院和助产机构)分娩的5120例新生儿进行听力和22个耳聋基因159个位点的联合筛查,及对基因突变或听力筛查未通过的新生儿在3月龄和6月龄进行听力学诊断。
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- 2021
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43. Additional file 1 of Genome-wide association study identifies new loci associated with risk of HBV infection and disease progression
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Zeng, Zheng, Hankui Liu, Huifang Xu, Haiying Lu, Yanyan Yu, Xiaoyuan Xu, Yu, Min, Zhang, Tao, Xiulan Tian, Hongli Xi, Liping Guan, Jianguo Zhang, and O’Brien, Stephen J.
- Abstract
Additional file 1: Figure S1. The summary of final SNPs characteristic, including MAF, call rate, and p-value of Hardy-Weinberg equilibrium test.. Figure S2. Principal component analyses indicated there are no population stratification among 6 subgroups. Abbreviation: ASPI, asymptomatic persistence infection; CHB, chronic hepatitis B; DC, decompensated cirrhosis; HC: healthy controls; HCC, hepatocellular carcinoma. Figure S3. Effective population sizes inferred using Related-package across all individuals of each population in two loci (HLA-DPA1, HLA-DPB1). Recentsize histories (26000 years ago) in European (purple) population showed modest difference compared with East Asian (red) population. Abbreviation: EUR, European; AMR, American; SAS, South Asian; AFR, African. Figure S4. Boxplots of rs2395166 genotype and serum liver enzyme levels in HC. Figure S5. Boxplots of rs615672 genotype and serum liver enzyme levels in HC. Figure S6. Boxplots of rs3077 genotype and serum liver enzyme levels in HC. Figure S7. Boxplots of rs1264473 genotype and serum liver enzyme levels in HC. Figure S8. Boxplots of rs2833856 genotype and serum liver enzyme levels in HC. Figure S9. Boxplots of rs6942409 genotype and serum liver enzyme levels in HC. Figure S10. The summary of associated SNPs contributed to HBV-related outcomes and the progression. Abbreviation: PI, persistence infection; ASPI, asymptomatic persistence infection; CHB, chronic hepatitis B; DC, decompensated cirrhosis; HCC, hepatocellular carcinoma. more...
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- 2021
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44. Exome sequencing and linkage analysis identified tenascin-C (TNC) as a novel causative gene in nonsyndromic hearing loss.
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Yali Zhao, Feifan Zhao, Liang Zong, Peng Zhang, Liping Guan, Jianguo Zhang, Dayong Wang, Jing Wang, Wei Chai, Lan Lan, Qian Li, Bing Han, Ling Yang, Xin Jin, Weiyan Yang, Xiaoxiang Hu, Xiaoning Wang, Ning Li, Yingrui Li, Christine Petit, Jun Wang, Huanming Yang Jian Wang, and Qiuju Wang more...
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Medicine ,Science - Abstract
In this study, a five-generation Chinese family (family F013) with progressive autosomal dominant hearing loss was mapped to a critical region spanning 28.54 Mb on chromosome 9q31.3-q34.3 by linkage analysis, which was a novel DFNA locus, assigned as DFNA56. In this interval, there were 398 annotated genes. Then, whole exome sequencing was applied in three patients and one normal individual from this family. Six single nucleotide variants and two indels were found co-segregated with the phenotypes. Then using mass spectrum (Sequenom, Inc.) to rank the eight sites, we found only the TNC gene be co-segregated with hearing loss in 53 subjects of F013. And this missense mutation (c.5317G>A, p.V1773M ) of TNC located exactly in the critical linked interval. Further screening to the coding region of this gene in 587 subjects with nonsyndromic hearing loss (NSHL) found a second missense mutation, c.5368A>T (p. T1796S), co-segregating with phenotype in the other family. These two mutations located in the conserved region of TNC and were absent in the 387 normal hearing individuals of matched geographical ancestry. Functional effects of the two mutations were predicted using SIFT and both mutations were deleterious. All these results supported that TNC may be the causal gene for the hearing loss inherited in these families. TNC encodes tenascin-C, a member of the extracellular matrix (ECM), is present in the basilar membrane (BM), and the osseous spiral lamina of the cochlea. It plays an important role in cochlear development. The up-regulated expression of TNC gene in tissue repair and neural regeneration was seen in human and zebrafish, and in sensory receptor recovery in the vestibular organ after ototoxic injury in birds. Then the absence of normal tenascin-C was supposed to cause irreversible injuries in cochlea and caused hearing loss. more...
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- 2013
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45. Exome sequencing of 47 chinese families with cone-rod dystrophy: mutations in 25 known causative genes.
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Li Huang, Qingyan Zhang, Shiqiang Li, Liping Guan, Xueshan Xiao, Jianguo Zhang, Xiaoyun Jia, Wenmin Sun, Zhihong Zhu, Yang Gao, Ye Yin, Panfeng Wang, Xiangming Guo, Jun Wang, and Qingjiong Zhang
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Medicine ,Science - Abstract
The goal of this study was to identify mutations in 25 known causative genes in 47 unrelated Chinese families with cone-rod dystrophy (CORD).Forty-seven probands from unrelated families with CORD were recruited. Genomic DNA prepared from leukocytes was analyzed by whole exome sequencing. Variants in the 25 genes were selected and then validated by Sanger sequencing.Fourteen potential pathogenic mutations, including nine novel and five known, were identified in 10 of the 47 families (21.28%). Homozygous, compound heterozygous, and hemizygous mutations were detected in three, four, or three families, respectively. The 14 mutations in the 10 families were distributed among CNGB3 (three families), PDE6C (two families), ABCA4 (one family), RPGRIP1 (one family), RPGR (two families), and CACNA1F (one family).This study provides a brief view on mutation spectrum of the 25 genes in a Chinese cohort with CORD. Identification of novel mutations enriched our understanding of variations in these genes and their associated phenotypes. To our knowledge, this is the first systemic exome-sequencing analysis of all of the 25 CORD-associated genes. more...
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- 2013
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46. Structural Features and Potent Antidepressant Effects of Total Sterols and β-sitosterol Extracted from Sargassum horneri
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Donghai Zhao, Lianwen Zheng, Ling Qi, Shuran Wang, Liping Guan, Yanan Xia, and Jianhui Cai
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Sargassum horneri ,sterols ,β-sitosterol ,FST ,TST ,monoamine neurotransmitters ,Biology (General) ,QH301-705.5 - Abstract
The purified total sterols and β-sitosterol extracted from Sargassum horneri were evaluated for their antidepressant-like activity using the forced swim test (FST) and tail suspension test (TST) in mice. Total sterols and β-sitosterol significantly reduced the immobility time in the FST and TST. Total sterols were administered orally for 7 days at doses of 50, 100, and 200 mg/kg, and β-sitosterol was administered intraperitoneally at doses of 10, 20, and 30 mg/kg. β-sitosterol had no effect on locomotor activity in the open field test. In addition, total sterols and β-sitosterol significantly increased NE, 5-HT, and the metabolite 5-HIAA in the mouse brain, suggesting that the antidepressant-like activity may be mediated through these neurotransmitters. more...
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- 2016
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47. Design and application of a water-soluble phosphorescent Ru(ii) complex as turn-on sensing material for Hg
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Jiaxi, Ru, Xiangquan, Mi, Liping, Guan, Xiaoliang, Tang, Zhenghua, Ju, Guolin, Zhang, Chunming, Wang, and Weisheng, Liu
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A novel phosphorescent chemodosimeter material Ruphen-1 based on a Ru(ii) complex has been designed and developed by introducing Hg
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- 2020
48. An integrated peach genome structural variation map uncovers genes associated with fruit traits
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Wenwu Guo, Lirong Wang, Xinwei Wang, Ke Cao, Jia-Long Yao, Liping Guan, Shan Wu, Changwen Chen, Cecilia H. Deng, Yong Li, Jian Guo, Weichao Fang, Zhangjun Fei, Jinlong Wu, and Gengrui Zhu
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0106 biological sciences ,Candidate gene ,lcsh:QH426-470 ,Fruit shape ,Genome-wide association study ,Biology ,01 natural sciences ,Genome ,Structural variation ,Anthocyanins ,03 medical and health sciences ,GWAS ,Domestication ,lcsh:QH301-705.5 ,Gene ,030304 developmental biology ,Genetic association ,Genetics ,Prunus persica ,0303 health sciences ,SVs ,Research ,Peach ,Human genetics ,lcsh:Genetics ,lcsh:Biology (General) ,Fruit ,Genomic Structural Variation ,Genome, Plant ,010606 plant biology & botany - Abstract
Background Genome structural variations (SVs) have been associated with key traits in a wide range of agronomically important species; however, SV profiles of peach and their functional impacts remain largely unexplored. Results Here, we present an integrated map of 202,273 SVs from 336 peach genomes. A substantial number of SVs have been selected during peach domestication and improvement, which together affect 2268 genes. Genome-wide association studies of 26 agronomic traits using these SVs identify a number of candidate causal variants. A 9-bp insertion in Prupe.4G186800, which encodes a NAC transcription factor, is shown to be associated with early fruit maturity, and a 487-bp deletion in the promoter of PpMYB10.1 is associated with flesh color around the stone. In addition, a 1.67 Mb inversion is highly associated with fruit shape, and a gene adjacent to the inversion breakpoint, PpOFP1, regulates flat shape formation. Conclusions The integrated peach SV map and the identified candidate genes and variants represent valuable resources for future genomic research and breeding in peach. more...
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- 2020
49. Adopting US Postgraduate Education to Promote High-Quality Marine Pharmaceutical Postgraduates in China
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Qinghao Jin, Haiyang Wang, and Liping Guan
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business.industry ,media_common.quotation_subject ,Political science ,Quality (business) ,Pharmacy ,Public relations ,business ,Investment (macroeconomics) ,China ,media_common ,Western education - Abstract
Postgraduate education in Postgraduate education in the United States is a typical example of Western education: it is a comparatively distinctive and successful education model. From an analysis of development trends in US postgraduate education and the postgraduate education model there, this paper aims to help improve the quality of postgraduate education in China, particularly with respect to marine pharmacy. This study concludes that the way to achieve that is through increased investment in scientific research and improved collaboration. more...
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- 2020
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50. Genomic analyses provide insights into peach local adaptation and responses to climate change
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Wenwu Guo, Junxiu Wang, Sanwen Huang, Pere Arús, Xiuli Zeng, Jiao Wang, Lirong Wang, Changwen Chen, Weichao Fang, Zhangjun Fei, Nan Li, Jian Guo, Qi Wang, Kuozhan Liu, Ke Cao, Shanshan Zhang, Yong Li, Liping Guan, Xinwei Wang, Tiyu Ding, and Gengrui Zhu more...
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0106 biological sciences ,2. Zero hunger ,0303 health sciences ,Candidate gene ,Natural selection ,Human evolutionary genetics ,15. Life on land ,Biology ,01 natural sciences ,Genome ,03 medical and health sciences ,13. Climate action ,Evolutionary biology ,Adaptation ,Selection (genetic algorithm) ,030304 developmental biology ,010606 plant biology & botany ,Local adaptation ,Genetic association - Abstract
The environment has constantly shaped plant genomes, but the genetic bases underlying how plants adapt to environmental influences remain largely unknown. We constructed a high-density genomic variation map by re-sequencing genomes of 263 geographically representative peach landraces and wild relatives. A combination of whole-genome selection scans and genome-wide environmental association studies (GWEAS) was performed to reveal the genomic bases of peach local adaptation to diverse climates comprehensively. A total of 2,092 selective sweeps that underlie local adaptation to both mild and extreme climates were identified, including 339 sweeps conferring genomic pattern of adaptation to high altitudes. Using GWEAS, a total of 3,496 genomic loci strongly associated with 51 specific environmental variables were detected. The molecular mechanism underlying adaptive evolution of high drought, strong UV-B, cold hardiness, sugar content, flesh color, and bloom date were revealed. Finally, based on 30 years of observation, a candidate gene associated with bloom date advance, representing peach responses to global warming, was identified. Collectively, our study provides insights into molecular bases of how environments have shaped peach genomes by natural selection and adds valuable genome resources and candidate genes for future studies on evolutionary genetics, adaptation to climate changes, and future breeding. more...
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- 2020
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