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AbstractAnticancer medications cause anemia in patients through ill-defined mechanisms, including hemolysis and eryptosis. Although α-linolenic acid (ALA) possesses anticancer properties against a variety of cancer cells, there is a dearth of evidence regarding how it modulates red blood cell (RBC) physiology. RBCs from healthy donors were subjected to anticancer concentrations of ALA (2.5, 5, 10, 20, 40, 80, and 100 μM) at 37 °C for 24 h, and colorimetric tests were used to determine hemolysis and acetylcholinesterase (AChE) activity. Meanwhile, flow cytometry was employed to identify eryptotic cells using annexin-V-FITC and forward scatter (FSC), Fluo4/AM to detect Ca2+, and H2DCFDA to assess oxidative stress. ALA significantly increased hemolysis and eryptosis in a concentration-dependent manner, along with elevated Fluo4 and DCF fluorescence, and erythrocyte sedimentation rate, and reduced FSC and AChE activity. Moreover, the addition of D4476, necrosulfonamide, melatonin, isosmotic urea, and polyethylene glycol 8000 – but not sucrose – significantly inhibited ALA toxicity. In conclusion, ALA stimulates hemolysis and eryptosis through Ca2+ buildup, oxidative stress, anticholinesterase activity, casein kinase 1α (CK1α), and mixed lineage kinase domain-like protein (MLKL). The anticancer activity of ALA may be potentiated by the use of Ca2+ channel blockers and chelators, antioxidants, and CK1α and MLKL inhibitors to ameliorate its toxicity to RBCs. [ABSTRACT FROM AUTHOR]

Photoautotrophic algal cultivation is challenging due to the shadowing effect produced by an increase in the number of cells; under such circumstances, mixotrophic growth might be an efficient alternative. We grew cultures of the autochthonous acidotolerant microalga Elliptochloris sp. on crude technical glycerin or glucose bubbled with either only air or air containing 2.5 % (v/v) CO2. We found that CO2 strongly influenced the production of Elliptochloris sp., as higher growth occurred in mixotrophy with CO2-enriched air compared to that with only air. Mixotrophy with CO2-enriched air allowed to reach higher biomass productivities and facilitated an increase in the relative abundance of saturated fatty acids. Nevertheless, mixotrophy with only air resulted in an increase of both saturated and unsaturated fatty acids, but biomass productivities were lower. These results, along with the limited biological contamination facilitated by low pH, suggest that this microalga might be attractive for large-scale production within the circular economy model. [ABSTRACT FROM AUTHOR]

The oil derived from Psidium guajava seeds (TKSO) exhibits an abundance of diverse unsaturated fatty acids, notably oleic, linoleic, and α-linolenic acids, conferring substantial health advantages in addressing metabolic irregularities and human diseases. This research endeavor focused on elucidating the impacts of TKSO on colonic inflammatory responses and intestinal microbiota alterations in a murine model of colitis induced by dextran sulfate sodium (DSS), demonstrated that substantial supplementation with TKSO reduces the severity of colitis induced by DSS. Furthermore, TKSO effectively attenuated the abundance and expression of proinflammatory mediators while augmenting the expression of tight junction proteins in DSS-challenged mice. Beyond this, TKSO intervention modulated the intestinal microbial composition in DSS-induced colitis mice, specifically by enhancing the relative presence of Lactobacillus, Norank_f_Muribaculaceae, and Lachnospiraceae_NK4A136_group, while concurrently diminishing the abundance of Turicibacter. Additionally, an analysis of short-chain fatty acids (SCFAs) revealed noteworthy elevations in acetic, propionic, isobutyric, and butyric acids, and total SCFAs levels in TKSO-treated mice. In summary, these findings underscore the potential of TKSO to reduce the severity of colitis induced by DSS in mice through intricate modulation of the intestinal microbiota, metabolite profiles, and intestinal barrier repair, thereby presenting a promising avenue for the development of therapeutic strategies against intestinal inflammatory conditions. [ABSTRACT FROM AUTHOR]

Understanding the nutritional diversity in Perilla (Perilla frutescens L.) is essential for selecting and developing superior varieties with enhanced nutritional profiles in the North Eastern Himalayan (NEH) region of India. In this study, we assessed the nutritional composition of 45 diverse perilla germplasm collected from five NEH states using standard protocols and advanced analytical techniques. Significant variability was observed in moisture (0.39–11.67%), ash (2.59–7.13%), oil (28.65–74.20%), protein (11.05–23.15%), total soluble sugars (0.34–3.67%), starch (0.01–0.55%), phenols (0.03–0.87%), ferric reducing antioxidant power (0.45–1.36%), palmitic acid (7.06–10.75%), stearic acid (1.96–2.29%), oleic acid (8.11–13.31%), linoleic acid (15.18–22.74%), and linolenic acid (55.47–67.07%). Similarly, significant variability in mineral content (ppm) was also observed for aluminium, calcium, cobalt, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, sodium, nickel, phosphorus, and zinc. Multivariate analyses, including hierarchical clustering analysis (HCA) and principal component analysis (PCA), revealed the enriched nutritional diversity within the germplasm. Correlation analysis indicated significant positive and negative relationships between nutritional parameters, indicating potential biochemical and metabolic interactions present in the perilla seeds. TOPSIS-based ranking identified promising genotypes for functional foods, pharmaceuticals, and nutritional applications. This study provides a first in-depth report of the nutritional composition and diversity of perilla germplasm in the NEH region, thus aiding in the identification of superior varieties for food and nutritional diversification and security. [ABSTRACT FROM AUTHOR]

Liquid state fermentation is now a commonly used route to obtain triterpenoids from Antrodia cinnamomea, and linolenic acid can significantly promote triterpenoids synthesis, whereas its action mechanism has not been studied. Here, we comprehensively performed an investigation on the mechanism of linolenic acid to promote triterpenoids production in liquid‐state fermentation of A. cinnamomea. Results showed that the addition of linolenic acid increased the unsaturated fatty acid index, fluidity, and permeability in the cell membrane of A. cinnamomea mycelia, favored the absorption of nutrients in the medium by the mycelium, enhanced the material exchange inside and outside, and thus promoted mycelial growth and triterpenoids synthesis. Moreover, 767 significantly differentially expressed genes were detected by adding linolenic acid, including 212 upregulated genes and 555 downregulated genes. The upregulated genes were mainly enriched in metabolism, glycolytic pathway, TCA cycle, and pyruvate metabolism. It was seen that the addition of linolenic acid improved the cell metabolic activity and promoted the synthesis of secondary metabolites, proving that the addition of linolenic acid improved the metabolic viability of cells and promoted secondary metabolite synthesis. [ABSTRACT FROM AUTHOR]

Background & Objective: The research was carried out in order to investigate the yield, yield components and quantitative and qualitative characteristics of the oil extracted from 19 double haploid lines of Camelina plant in the rainfed conditions of Kermanshah. Material and Methods: A field experiment was carried out as randomized complete block design (RCBD) with 20 treatments and three replications at the Serarud Rainfed Agricultural Research Institute of Kermanshah, Iran in 2021 growing season. The experimental treatments included Soheil cultivar as control and 19 double haploid lines (15 different crosses that were produced by the method of anther cultivation in Razi University of Kermanshah, Iran). Results: The results demonstrated that the highest seed yield (345.2 kg/ha) of camelina was obtained in DH100 line, which showed an increase of 43.5% compared to the control variety. Also, the lowest seed yield was related to DH105 line, which showed a decrease of 67.3% compared to the control variety. In addition, the highest oil percentage and oil yield were obtained in DH100 line, which showed an increase of 2.8% and 34.1% compared to the control variety. Chemical analysis of camelina oil showed that the major fatty acids constituents in this plant's oil include linolenic acid (31.16-34.69%), linoleic acid (16.74-18.93%), oleic acid (14.12-17-31.) and eicosenoic acid (9.51-11.39). The highest amount of oleic, linoleic and linolenic acid was observed in DH100 line. In addition, the highest amount of eicosenoic acid belonged to the DH10 followed by DH100 line. It is worth mentioning that the amount of oleic, linoleic, linolenic and eicosenoic fatty acids increased by 10.9, 5.6, 6.4 and 8.7%, respectively, when compared with control variety. Conclusion: Generally, it can be concluded that the DH100 line is recommended as the best line for releasing new cultivars of this oil-seed crop in rainfed conditions due to the highest oil quantity and quality in comparison with control variety and other double haploid lines. [ABSTRACT FROM AUTHOR]

Grape seeds are rich in bioactive substances, including polyphenols, terpenoids, and phytosterols. Linseed (Linum usitatissimum L.) boasts a high concentration of polyunsaturated fatty acids (PUFAs), lignans, phytoestrogens, and soluble fibers, all contributing to its therapeutic potential. In this study, we pioneered the formulation of an oil blend (GL) combining grape seed oil (G) and golden linseed oil (GL) in equal volumes (1:1 (v/v)) and we evaluated in terms of the nutritional, physical, and chemical properties and their influence in an in vivo experimental model. We analyzed the oils by performing physical–chemical analyses, examining the oxidative stability using Rancimat; conducting thermal analyses via thermogravimetry/derivative thermogravimetry (TG/DTG) and differential scanning calorimetry (DSC), performing optical UV–vis absorption analyses; examining the fluorescence emission–excitation matrix, total carotenoids, and color, and conducting metabolic assessments in an in vivo experimental trial. The fatty acid profile presented a higher fraction of linoleic acid (C18:2) in G and GL and alpha-linolenic acid (C18:3) in L. The acidity and peroxide indices were within the recommended ranges. The TG/DTG, DSC, and Rancimat analyses revealed similar behaviors, and the optical analyses revealed color variations caused by carotenoid contents in L and GL. In the in vivo trial, G (G2: 2000 mg/kg/day) promoted lower total consumption, and the blend (GL: 2000 mg/kg/day) group exhibited less weight gain per gram of consumed food. The group with G supplementation (G2: 2000 mg/kg/day) and GL had the highest levels of HDL-c. The group with L supplementation (L2: 2000 mg/kg/day) had the lowest total cholesterol level. The L2, G1 (1000 mg/kg/day), and G2 groups exhibited the lowest MCP-1 and TNF-α values. Additionally, the lowest adipocyte areas occurred in G and GL. Our results suggest that this combination is of high quality for consumption and can influence lipid profiles, markers of inflammation, and antioxidant status. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan University of Technology Natural Science Edition is the property of Henan University of Technology Journal Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Dry bean (Phaseolus vulgaris L.) is a crop of high nutritional interest widespread throughout the world. This research had two objectives. On the one hand, the development and validation of an analytical method to quantify fatty acids in dry beans based on the extraction and derivatization in a single step and later quantification by gas chromatography. On the other, its application to characterize the fatty acid content in a diversity panel consisting of 172 lines. The method was successfully validated in terms of accuracy, precision and robustness. Among the 14 fatty acids that constitute the fatty acid profile of dry bean, the most quantitatively important were linolenic acid, the major fatty acid in all cases, with an average value of 6.7 mg/g, followed by linoleic acid (3.9 mg/g), palmitic acid (2.9 mg/g) and oleic acid (1.5 mg/g). The concentrations of fatty acids in dry bean were influenced by the gene pool, with the Mesoamerican gene pool showing a higher content of palmitic, stearic, linoleic and linolenic acids and the Andean gene pool a higher level of cis-vaccenic acid. Also, the expression of fatty acid content showed high heritability. The information generated constitutes a robust database of interest in food technology, nutrition and breeding programs. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In a bid to explore and enhance the use of natural products for medicinal purposes, this study analyzed the crude extract of Ricinodendron heudelotii seeds and isolated four biologically active compounds from ethyl acetate fraction of R. heudelotii seeds extract. The study utilized GC-MS in the analysis of the crude extract. Various spectroscopic and spectrometric methods in addition to reported data were employed in characterizing isolates. Twenty-two compounds were detected in the crude extract via GC-MS. Palmitic acid had the highest abundance of 23.9%, followed by α-linolenic acid (14.72%). The ethyl acetate fraction by means of chromatographic techniques yielded four compounds namely glucogallin (1), (6Z,9Z,11Z)-6,9,11-octadecatrienoic acid (2), (6Z,9E,11E)-6,9,11-octadecatrienoic acid (3) and (6Z,9Z,13Z)-6,9,13-octadecatrienoic acid (4). All four isolated compounds have biological and pharmaceutical properties including anti-inflammatory and anti-diabetic. This study, therefore, proposes linolenic acid and gallic acid derivatives as chemophenetic markers of R. heudelotii and suggests the medicinal use of R. heudelotii seeds for anti-inflammatory and anti-diabetic disorders. [ABSTRACT FROM AUTHOR]

Abstract The objective was to evaluate the oxidative stability of flaxseed cake and its inclusion in the diet of lactating cows, on milk production and composition and blood parameters (total cholesterol, triglycerides, high density lipoprotein -HDL and low density lipoprotein - LDL). Oxidative stability was evaluated by acidity index (AI) and peroxides (PI), determined from day zero to the ninth day of storage. Eighteen crossbreed Holstein x Jersey cows were used, nine animals per treatment (without and with the inclusion of cake). The experimental design was completely randomized, with two treatments and nine replications. There was an increase in PI during the first six days of storage, followed by a decrease on the ninth day. Supplying 4.5% flaxseed cake to lactating cows for 90 days did not promote changes in production, chemical composition and fatty acid profile of milk, somatic cell counts and blood parameters. Thus, flaxseed cake can be used to feed lactating cows.

One of the main components of sustainable agricultural strategies in crop production is substitution of chemical fertilizers with alternatives. This study aimed to examine the effects of partial replacement of mineral fertilizers with bio-stimulants (yeast and/or moringa leaves extract). Field experiment was conducted during two successive seasons 2018/2019 and 2019/2020. The fertilization treatments were distributed in a factorial design. Treatments included 75%, 50% or 0% NPK combined with yeast, moringa extract, or their combination in comparison with control treatment (100% NPK). The partial substitution of mineral fertilizers with yeast and/or moringa extract had significant impacts on growth, yield and quality components. Decreasing NPK level significant decreased growth and yield components of chia plants, except for 75% NPK combined with yeast and/or moringa. In contrast, decreasing NPK level significantly increased the chemical and quality components of chia. Treating with 75% NPK combined with yeast and/or moringa gave the highest micronutrients and fatty acid content. GC analysis showed definition of nine main components, and the major components were linolenic and linoleic acids. Linolenic acid increased with decreasing NPK, and the highest values obtained with bio-stimulant treatments with zero % NPK. The results of this study recommended that partial substitution of 25% of mineral fertilizers with bio-stimulants (yeast and/or moringa) is the best alternative not only for maintaining the growth and yield component, but also improving the quality of chia plants. [ABSTRACT FROM AUTHOR]

Bakery products have gained prominence in modern diets due to their convenience and accessibility, often serving as staple meals across diverse regions. However, the fats used in these products are rich in saturated fatty acids and often comprise trans fatty acids, which are considered as a major biomarker for non‐communicable diseases like cardiovascular disorders, obesity and diabetes. Additionally, these fats lack the essential omega‐3 fatty acids, which are widely known for their therapeutic benefits. They play a major role in lowering the risk of cardiovascular diseases, cancer and diabetes. Thus, there is need for incorporating these essential fatty acids into bakery fats. Nevertheless, fortifying food products with polyunsaturated fatty acids (PUFAs) poses several challenges due to their high susceptibility to oxidation. This oxidative deterioration leads to not only the formation of undesirable flavors, but also a loss of nutritional value in the final products. This review focuses on the development of healthier trans‐fat‐free bakery fat enriched with omega‐3 fatty acids and its effect on the physicochemical, functional, sensory and nutritional properties of bakery fats and products. Further, the role of various technologies like physical blending, enzymatic interesterification and encapsulation to improve the stability of PUFA‐rich bakery fat is discussed, where microencapsulation emerged as a novel and effective technology to enhance the stability and shelf life. By preventing deteriorative changes, microencapsulation ensures that the nutritional, physicochemical and sensory properties of food products remain intact. Novel modification methods like interesterification and microencapsulation used for developing PUFA‐rich bakery fats have a potential to address the health risks occurring due to consumption of bakery fat having higher amount of saturated and trans fatty acids. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

α-Linolenic acid (ALA) is an important nutrient component in rapeseed oil, and rapeseed breeders want to either restrain or enhance the function of fatty acid desaturases (FADs) in the ALA biosynthesis pathway. To determine the reason for the upregulation of rapeseed BnFAD genes in two high-ALA accessions, R8Q10 and YH25005, we compared their transcriptome profiles in the seed at 24 days after pollination (DAP) with those of two low-ALA lines, A28 and SW. The expression levels of twenty-eight important genes in the seed samples at 20, 27, and 34 DAP were also investigated using an RT-qPCR. The expression levels of genes involved in flavonoid and proanthocyanidin synthesis, including BnCHS, BnCHI, BnDFR, BnFLS1, BnLDOX, BnBAN, BnTT10, and BnTT12 and genes encoding the transcription factors BnTT1, BnTT2, BnTT8, and BnTT16 were lower in R8Q10 and YH25005 than in A28 and SW. The expression levels of genes encoding master transcription factors in embryo development, such as BnLEC1, BnABI3, BnFUS3, BnL1L, BnAREB3, and BnbZIP67, were elevated significantly in the two high-ALA accessions. Combined with previous results in the Arabidopsis and rapeseed literature, we speculated that the yellow-seededness genes could elevate the activity of BnLEC1, BnABI3, BnFUS3, and BnbZIP67, etc., by reducing the expression levels of several transparent testa homologs, resulting in BnFAD3 and BnFAD7 upregulation and the acceleration of ALA synthesis. Yellow-seededness is a favorable factor to promote ALA synthesis in the two high-ALA accessions with the yellow-seeded trait. These findings provide initial insights into the transcriptomic differences between high-/low-ALA germplasms and a theoretic basis for seed quality breeding. [ABSTRACT FROM AUTHOR]

Copyright of Medicina (Buenos Aires) is the property of Medicina (Buenos Aires) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The fatty acid (FA) compositions of thirty-nine vegetable oils and fats, including nangai nut, pili nut, shea butter, tamanu oil, baobab, sea buckthorn berry, Brazil nut, grape seed, black seed, evening primrose, passion fruit, milk thistle, sunflower, pumpkin seed, sesame, soybean, flax seed, kukui, red raspberry seed, walnut, chia seed, hemp seed, rosehip, almond, avocado, carrot seed, moringa, apricot kernel, camellia seed, macadamia, olive, marula, argan, castor, jojoba, pomegranate seed, medium-chain triglyceride (MCT) coconut, roasted coconut, canola, and mustard oil, were analyzed using gas chromatography–mass spectrometry (GC-MS). Vegetable oils and fats have different profiles in terms of their fatty acid composition, and their major constituents vary significantly. However, we categorized them into different classes based on the percentages of different fatty acids they contain. The saturated fatty acids, such as palmitic acid and stearic acid, and the unsaturated fatty acids, including oleic acid, linoleic acid, and linolenic acid, are the main categories. Among them, roasted coconut oil contained the greatest amount of saturated fatty acids followed by nangai nut (45.61%). Passion fruit oil contained the largest amount of linoleic acid (66.23%), while chia seed oil had the highest content of linolenic acid (58.25%). Oleic acid was exclusively present in camellia seed oil, constituting 78.57% of its composition. Notably, mustard oil had a significant presence of erucic acid (54.32%), while pomegranate seed oil exclusively contained punicic acid (74.77%). Jojoba oil primarily consisted of (Z)-11-eicosenoic acid (29.55%) and (Z)-docos-13-en-1-ol (27.96%). The major constituent in castor oil was ricinoleic acid (89.89%). Compared with other vegetable oils and fats, pili nut oil contained a significant amount of (E)-FA (20.62%), followed by sea buckthorn berry oil with a content of 9.60%. FA compositions from sources may be problematic in the human diet due to no labeling or the absence of essential components. Therefore, consumers must cast an eye over some essential components consumed in their dietary intake. [ABSTRACT FROM AUTHOR]

Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW. The three high-ALA accessions shared similar sequences with the promoters and CDSs of BnFAD3.C4 and BnFAD3.A3. In A28 and SW, substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5, an absence of BnFAD2.C1 locus, and a 549 bp long deletion on the BnFAD3.A3 promoter were detected. The profile of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies. The mutations in BnFAD3 in the high-ALA accessions are reported for the first time. In identifying the sites of these mutations, we provide detailed information to aid the design of molecular markers for accelerated breeding schemes. [ABSTRACT FROM AUTHOR]

In this study, we applied exogenous chlorogenic acid (CGA) to Lonicera japonica (L. japonica) leaves via foliar sprays every Monday, Wednesday, and Friday for a period of 12 months. Our continuous monitoring over this period revealed a consistent increase in flavonoid levels from the second to the tenth month following the commencement of CGA treatment. This was accompanied by a notable upregulation in the expression of four secondary metabolite-related enzyme genes: LjPAL1, LjPAL2, LjPAL3, and LjISY1. Concurrently, there was a significant enhancement in the total activity of the enzyme phenylalanine ammonia-lyase. The total antioxidant capacity of the plants also showed a marked increase from the third to the seventh month post-treatment initiation, subsequently stabilizing. This increase was also reflected in the elevated activities of key antioxidant enzymes: peroxidase, polyphenol oxidase, and superoxide dismutase. Furthermore, the treatment notably enhanced various indicators of nutrient growth, such as total protein content, total sugar content, and leaf area. Notably, the relative expression of LjTF1, a kind of BZIP transcription factor gene known for its extensive regulatory effects, showed a significant and sustained increase after the start of exogenous CGA treatment. Subsequent metabolomic analysis revealed significant changes in L. japonica metabolites. Specifically, 172 differentially expressed metabolites (DEMs) showed a notable increase (Fold > 1), predominantly in pathways related to nutrient metabolism such as carbohydrate, amino acid, and energy metabolism. Notably, some of the highly expressed DEMs (Fold > 4) are key antioxidants and medicinal components in L. japonica. The experimental findings were in alignment with the metabolomics analysis, indicating that exogenous CGA can act as a stimulant for L. japonica. It promotes the significant accumulation of certain secondary metabolites, enhances nutritive growth, and boosts the plant's total antioxidant capacity. [ABSTRACT FROM AUTHOR]

The study aims to develop a micronutrients and bioactive compounds rich, new malted drink formula using malted green gram (Vigna radiata), soybeans (Glycine max), watermelon (Citrullus lanatus) seed kernel and ginger (Zingiber officinale) rhizomes. The sensory analysis data revealed that the formula which contains 52.17 % of soybean flour, 26.00 % of malted green gram flour, 17.39 % of watermelon seed kernel flour and 4.34 % of ginger powder has the highest overall acceptability. This selected formula contained moisture (3.27 ± 0.29 %), total ash (3.96 ± 0.39 %), total fat (12.93 ± 0.19 %), protein (33.58 ± 0.64 %), crude fiber (2.70 ± 0.49 %), carbohydrate (43.56 ± 0.89 %), reducing sugars (4.98 ± 0.13 %) and dietary fiber (18.67 ± 0.36 %). Considerable amounts (mg/100 g) of Potassium (2332.19 ± 5.69), Phosphorus (1003.78 ± 9.12) and Magnesium (374.07 ± 3.36) were reported. The fatty acid profile was dominated by polyunsaturated fatty acids (60.53 %) including Linoleic acid (56.40 %) and α-Linolenic Acid (4.13 %). The detected amounts of prominent micronutrients and α-Linolenic Acid adequately comply with the Recommended Dietary Allowance (RDA) values. Moreover, the new formula possessed 2.3 ± 0.11 mg GAE/g of total phenolic compounds and exhibited significantly high DPPH (12.81 ± 2.19 mg/ml) and ABTS (25.16 ± 0.41 mg/ml) radical scavenging activities.

The fatty acid (FA) compositions of thirty-nine vegetable oils and fats, including nangai nut, pili nut, shea butter, tamanu oil, baobab, sea buckthorn berry, Brazil nut, grape seed, black seed, evening primrose, passion fruit, milk thistle, sunflower, pumpkin seed, sesame, soybean, flax seed, kukui, red raspberry seed, walnut, chia seed, hemp seed, rosehip, almond, avocado, carrot seed, moringa, apricot kernel, camellia seed, macadamia, olive, marula, argan, castor, jojoba, pomegranate seed, medium-chain triglyceride (MCT) coconut, roasted coconut, canola, and mustard oil, were analyzed using gas chromatography–mass spectrometry (GC-MS). Vegetable oils and fats have different profiles in terms of their fatty acid composition, and their major constituents vary significantly. However, we categorized them into different classes based on the percentages of different fatty acids they contain. The saturated fatty acids, such as palmitic acid and stearic acid, and the unsaturated fatty acids, including oleic acid, linoleic acid, and linolenic acid, are the main categories. Among them, roasted coconut oil contained the greatest amount of saturated fatty acids followed by nangai nut (45.61%). Passion fruit oil contained the largest amount of linoleic acid (66.23%), while chia seed oil had the highest content of linolenic acid (58.25%). Oleic acid was exclusively present in camellia seed oil, constituting 78.57% of its composition. Notably, mustard oil had a significant presence of erucic acid (54.32%), while pomegranate seed oil exclusively contained punicic acid (74.77%). Jojoba oil primarily consisted of (Z)-11-eicosenoic acid (29.55%) and (Z)-docos-13-en-1-ol (27.96%). The major constituent in castor oil was ricinoleic acid (89.89%). Compared with other vegetable oils and fats, pili nut oil contained a significant amount of (E)-FA (20.62%), followed by sea buckthorn berry oil with a content of 9.60%. FA compositions from sources may be problematic in the human diet due to no labeling or the absence of essential components. Therefore, consumers must cast an eye over some essential components consumed in their dietary intake.

Oil flax seeds are valuable raw material for various industries. The purpose of the research was to form a genetic collection of oil flax for resistance to Fusarium wilt in order to create specialized varieties for various purposes with highly effective R-genes. On infectious backgrounds from populations of the fungus Fusarium oxysporum f. lini (Bolley) and highly virulent monoisolate No 39 of this pathogens, 28 promising genotypes of oilseed flax were assessed. Using a phytopathological test from the world gene pool of the species Linum usitatissimum L. 10 promising oil flax genotypes were identified – No. 3829, Uralsky (Russia), l. 6 AGT 1538, l. 12 AGT 987 (Czech Republic), Altess, Eole (France), Selectiong 3 (China), Arnu, Bison (USA) and Linda (Canada), which have effective resistance genes to the fungus F. oxysporum f. lini, differing in the fatty acid composition of the oil. Using the method of hybridological analysis, it has been established that resistance to Fusarium wilt in the low linolenic line l. 12 AGT 987 (Czech Republic) is determined by the Fu 11 gene, while in the high linolenic l. 3 Altess (France) it is Fu 4 (χ2 = 0.02…2.89). In the low linolenic line l. 6 AGT 1538 (Czech Republic) a previously unknown gene for resistance to this disease – Fu 12 was identified (χ2 = 0.0001…2.4100). The use of identified genetic sources in breeding programs will contribute to the creation of specialized varieties for the food, chemical and other industries with various R-genes of resistance, which will prevent the development of epiphytoties of this disease on oil flax.

Walnut production is expanding worldwide due to the high demand for natural products with proven nutraceutical properties. This expansion includes new growing areas, such as central–western Argentina, where crop water requirements have yet to be determined and little is known about the response in terms of production quality to the water regime. The aim of this study was to assess the effects of four irrigation regimes (at 50, 75, 100, and 125 % of crop evapotranspiration) and the weather conditions over two consecutive seasons in a young Chandler walnut orchard in terms of in–shell and kernel caliber, kernel color, oil concentration, and fatty acid profile. Quality production characteristics were not significantly affected by irrigation regimes within each season. In contrast, the quality parameters achieved were significantly different between seasons. The first season (S1) was wetter (462 mm) and cooler (17.05 °C mean air temperature in the oil accumulation period) and produced more extra–light kernels (92%). The second season (S2) was drier (326 mm) and warmer (19.02 °C) and produced a lower proportion of extra–light kernels (82.2%). In addition, during the second season, walnuts produced larger kernels with a higher oil concentration (62.7%; +3.5% of S1) and a better omega–6/omega–3 ratio (3.25 in S2 vs. 3.70 in S1). These results suggest that kernel quality was more sensitive to the seasonal temperature than the irrigation studied regimes. The nutritional quality of walnuts may increase significantly in warmer seasons/environments despite the deterioration of kernel color.

With the increasing public attention to the health benefit of polyunsaturated fatty acids (PUFAs) and demand for linolenic acid (C18:3), it is of great significance to increase the C18:3 content in our meal. As an oil crop with high content of C18:3, Camelina sativa has three homologous copies of FAD2 and three homologous copies FAD3. In this study, we seed-specifically overexpressed two Camelina sativa fatty acid desaturase genes, CsFAD2 and CsFAD3, in rapeseed cultivar Zhongshuang 9. The results show that C18:3 content in CsFAD2 and CsFAD3 overexpressed seeds is increased from 8.62% in wild-type (WT) to 10.62–12.95% and 14.54–26.16%, respectively. We crossed CsFAD2 and CsFAD3 overexpression lines, and stable homozygous digenic crossed lines were obtained. The C18:3 content was increased from 8.62% in WT to 28.46–53.57% in crossed overexpression lines. In addition, we found that the overexpression of CsFAD2 and CsFAD3 had no effect on rapeseed growth, development, and other agronomic traits. In conclusion, we successfully generated rapeseed germplasms with high C18:3 content by simultaneously overexpressing CsFAD2 and CsFAD3, which provides a feasible way for breeding high C18:3 rapeseed cultivars. [ABSTRACT FROM AUTHOR]

Polyunsaturated Fatty Acids (PUFAs) are important nutrients for human health. We aimed to evaluate the efficiency of marine water fungus Aspergillus sp. (Accession no: MZ505709) for lipid biosynthesis. The Yeast Extract Glucose (YEG) medium was supplemented with different concentration of Borassus flabellifer Endocarps Hydrolysate (BFEH; 1–5%) to evaluate the fungal biomass and its lipid accumulation. The combination of glucose and BFEH as carbon source increased the fresh weight (25.43 ± 0.33 g/L), dry weight (21.39 ± 0.77 g/L) and lipid yield (3.14 ± 0.09 g/L) of fungal biomass. The lipid content of dried fungal biomass has shown 91.08 ± 5.07 mg cod liver oil equivalents/g and 125.98 ± 5.96 mg groundnut oil equivalents/g biomass. GC-MS and NMR spectrometry analysis revealed the compounds involved in fatty acid metabolism and lipid signaling pathways along with the presence of linolenic acid. Interestingly, fungus grown in BFEH enriched medium has recorded the maximum amount of lipids with major fatty acid derivatives. Increase in the growth rate of Artemia franciscana was observed, when the extracted fungal lipid was supplemented as a food supplement. Therefore, this study suggests that marine fungal lipid may serve as potential natural compound as nutraceuticals and aquafeeds. [ABSTRACT FROM AUTHOR]

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[Display omitted] Walnut production is expanding worldwide due to the high demand for natural products with proven nutraceutical properties. This expansion includes new growing areas, such as central–western Argentina, where crop water requirements have yet to be determined and little is known about the response in terms of production quality to the water regime. The aim of this study was to assess the effects of four irrigation regimes (at 50, 75, 100, and 125 % of crop evapotranspiration) and the weather conditions over two consecutive seasons in a young Chandler walnut orchard in terms of in–shell and kernel caliber, kernel color, oil concentration, and fatty acid profile. Quality production characteristics were not significantly affected by irrigation regimes within each season. In contrast, the quality parameters achieved were significantly different between seasons. The first season (S1) was wetter (462 mm) and cooler (17.05 °C mean air temperature in the oil accumulation period) and produced more extra–light kernels (92%). The second season (S2) was drier (326 mm) and warmer (19.02 °C) and produced a lower proportion of extra–light kernels (82.2%). In addition, during the second season, walnuts produced larger kernels with a higher oil concentration (62.7%; +3.5% of S1) and a better omega–6/omega–3 ratio (3.25 in S2 vs. 3.70 in S1). These results suggest that kernel quality was more sensitive to the seasonal temperature than the irrigation studied regimes. The nutritional quality of walnuts may increase significantly in warmer seasons/environments despite the deterioration of kernel color. [ABSTRACT FROM AUTHOR]

One of the main components of sustainable agricultural strategies in crop production is substitution of chemical fertilizers with alternatives. This study aimed to examine the effects of partial replacement of mineral fertilizers with bio-stimulants (yeast and/or moringa leaves extract). Field experiment was conducted during two successive seasons 2018/2019 and 2019/2020. The fertilization treatments were distributed in a factorial design. Treatments included 75%, 50% or 0% NPK combined with yeast, moringa extract, or their combination in comparison with control treatment (100% NPK). The partial substitution of mineral fertilizers with yeast and/or moringa extract had significant impacts on growth, yield and quality components. Decreasing NPK level significant decreased growth and yield components of chia plants, except for 75% NPK combined with yeast and/or moringa. In contrast, decreasing NPK level significantly increased the chemical and quality components of chia. Treating with 75% NPK combined with yeast and/or moringa gave the highest micronutrients and fatty acid content. GC analysis showed definition of nine main components, and the major components were linolenic and linoleic acids. Linolenic acid increased with decreasing NPK, and the highest values obtained with bio-stimulant treatments with zero % NPK. The results of this study recommended that partial substitution of 25% of mineral fertilizers with bio-stimulants (yeast and/or moringa) is the best alternative not only for maintaining the growth and yield component, but also improving the quality of chia plants.

Seed yield in soybean is a complex trait and is influenced by multiple genetic and environmental factors. Safflower is one of the oilseeds adapted to a wide range of soil conditions, but micronutrients deficiencies cause a decrease in its grain yield and quality. Hence, an experiment was conducted as a split factorial based on randomized complete block design with three replications at Agricultural and Natural Resources Research Center of Arak, Iran in 2019. Three tillage methods (including no tillage, low tillage and conventional tillage) were considered as the main plots and fertilizer treatments (including 1- Control, 2- Zinc, 3- Iron, 4- Sulfur, 5- Zinc + Iron, 6-Zinc + Sulfur, 7- Iron + Sulfur, and 8- Iron + Sulfur + Zinc) were considered as sub-plots. Results showed that there were significant differences between the fertilizer treatments in terms of seed yield, flower yield, seed weight per head, number of heads, number of seeds per head, percentage of seed protein and seed oil. In the treatment of tillage methods, thousand seed weight and linoleic fatty acid had significant differences. Interaction effects of soil tillage methods X fertilizer were significant on number of heads and linolenic acid. Fertilizer treatment of iron + sulfur + zinc had the highest seed yield (i.e. 1391 kg ha-1), flower yield (i.e. 183.7 kg ha-1), 1000 seed weight (i.e. 45.8 g), seed weight per head (i.e. 1.27 g), number of heads (i.e. 7.9), protein percentage (i.e. 21.2%) and seed oil percentage (i.e. 26.5). Results of this research showed that application of micronutrient elements by increasing the yield components, particularly 1000-seed weight, number of heads, and the weight of seeds per head led to increases in seed yield, oil percentage and protein percentage of safflower seeds. Though, tillage methods had no significant effect on safflower seed yield. According to the results of this experiment the use of zinc, sulfur, and iron fertilizers along with the method of low tillage can help to increase the grain yield and quality of rapeseed.

The oil derived from Psidium guajava seeds (TKSO) exhibits an abundance of diverse unsaturated fatty acids, notably oleic, linoleic, and α-linolenic acids, conferring substantial health advantages in addressing metabolic irregularities and human diseases. This research endeavor focused on elucidating the impacts of TKSO on colonic inflammatory responses and intestinal microbiota alterations in a murine model of colitis induced by dextran sulfate sodium (DSS), demonstrated that substantial supplementation with TKSO reduces the severity of colitis induced by DSS. Furthermore, TKSO effectively attenuated the abundance and expression of proinflammatory mediators while augmenting the expression of tight junction proteins in DSS-challenged mice. Beyond this, TKSO intervention modulated the intestinal microbial composition in DSS-induced colitis mice, specifically by enhancing the relative presence of Lactobacillus, Norank_f_Muribaculaceae, and Lachnospiraceae_NK4A136_group, while concurrently diminishing the abundance of Turicibacter. Additionally, an analysis of short-chain fatty acids (SCFAs) revealed noteworthy elevations in acetic, propionic, isobutyric, and butyric acids, and total SCFAs levels in TKSO-treated mice. In summary, these findings underscore the potential of TKSO to reduce the severity of colitis induced by DSS in mice through intricate modulation of the intestinal microbiota, metabolite profiles, and intestinal barrier repair, thereby presenting a promising avenue for the development of therapeutic strategies against intestinal inflammatory conditions.

Grape seeds are rich in bioactive substances, including polyphenols, terpenoids, and phytosterols. Linseed (Linum usitatissimum L.) boasts a high concentration of polyunsaturated fatty acids (PUFAs), lignans, phytoestrogens, and soluble fibers, all contributing to its therapeutic potential. In this study, we pioneered the formulation of an oil blend (GL) combining grape seed oil (G) and golden linseed oil (GL) in equal volumes (1:1 (v/v)) and we evaluated in terms of the nutritional, physical, and chemical properties and their influence in an in vivo experimental model. We analyzed the oils by performing physical–chemical analyses, examining the oxidative stability using Rancimat; conducting thermal analyses via thermogravimetry/derivative thermogravimetry (TG/DTG) and differential scanning calorimetry (DSC), performing optical UV–vis absorption analyses; examining the fluorescence emission–excitation matrix, total carotenoids, and color, and conducting metabolic assessments in an in vivo experimental trial. The fatty acid profile presented a higher fraction of linoleic acid (C18:2) in G and GL and alpha-linolenic acid (C18:3) in L. The acidity and peroxide indices were within the recommended ranges. The TG/DTG, DSC, and Rancimat analyses revealed similar behaviors, and the optical analyses revealed color variations caused by carotenoid contents in L and GL. In the in vivo trial, G (G2: 2000 mg/kg/day) promoted lower total consumption, and the blend (GL: 2000 mg/kg/day) group exhibited less weight gain per gram of consumed food. The group with G supplementation (G2: 2000 mg/kg/day) and GL had the highest levels of HDL-c. The group with L supplementation (L2: 2000 mg/kg/day) had the lowest total cholesterol level. The L2, G1 (1000 mg/kg/day), and G2 groups exhibited the lowest MCP-1 and TNF-α values. Additionally, the lowest adipocyte areas occurred in G and GL. Our results suggest that this combination is of high quality for consumption and can influence lipid profiles, markers of inflammation, and antioxidant status.

Dry bean (Phaseolus vulgaris L.) is a crop of high nutritional interest widespread throughout the world. This research had two objectives. On the one hand, the development and validation of an analytical method to quantify fatty acids in dry beans based on the extraction and derivatization in a single step and later quantification by gas chromatography. On the other, its application to characterize the fatty acid content in a diversity panel consisting of 172 lines. The method was successfully validated in terms of accuracy, precision and robustness. Among the 14 fatty acids that constitute the fatty acid profile of dry bean, the most quantitatively important were linolenic acid, the major fatty acid in all cases, with an average value of 6.7 mg/g, followed by linoleic acid (3.9 mg/g), palmitic acid (2.9 mg/g) and oleic acid (1.5 mg/g). The concentrations of fatty acids in dry bean were influenced by the gene pool, with the Mesoamerican gene pool showing a higher content of palmitic, stearic, linoleic and linolenic acids and the Andean gene pool a higher level of cis-vaccenic acid. Also, the expression of fatty acid content showed high heritability. The information generated constitutes a robust database of interest in food technology, nutrition and breeding programs.

Camelina oil is highly unsaturated and is a rich source of omega-3 fatty acids. Camelina oil contains high amounts of tocopherol which confers a reasonable shelf life without the need for special storage conditions. Generally crud oils contain many unwanted matters which must be removed to yield a stable product with a bland or pleasant taste. Chemical refining includes degumming, neutralization, bleaching and deodorization as separate process. In this study, the qualitative (FFA and PV) and compositional characteristics of camelina oil obtained from four regions of Ilam, Kermanshah, Hamedan and Fars were investigated after chemical refining operations. Changes in composional specifications were also evaluated before and after the final stage of refining, deodorization.Oils were extracted from camelina seeds from above region by soxhlet method, The extracted oils were refined. Fatty acid methyl ester (FAME), tocopherols FFA and PV were determined. Collected data was subjected to a one- way analysis of variance and Duncan's posthoc at the P

Linolenic acid (LA) has poor oxidative stability since it is a polyunsaturated fatty acid. Soybean oil has a high LA content and thus has poor oxidative stability. To identify candidate genes that affect the linolenic acid (LA) content in soybean seeds, a genome-wide association study (GWAS) was performed with 1,060 soybean cultivars collected in China between 2019-2021 and which LA content was measured using matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF IMS). A candidate gene, GmWRI14, encoding an APETALA2 (AP2)-type transcription factor, was detected by GWAS in cultivars from all three study years. Multiple sequence alignments showed that GmWRI14 belongs to the plant WRI1 family. The fatty acid contents of different soybean lines were evaluated in transgenic lines with a copy of GmWRI14, control lines without GmWRI14, and the gmwri14 mutant. MALDI-TOF IMS revealed that GmWRI14 transgenic soybeans had a lower LA content with a significant effect on seed size and shape, whereas gmwri14 mutants had a higher LA content. compared to control. The RNA-seq results showed that GmWRI14 suppresses GmFAD3s (GmFAD3B and GmFAD3C) and GmbZIP54 expression in soybean seeds, leading to decreased LA content. Based on the RNA-seq data, yeast one-hybrid (Y1H) and qRT-PCR were performed to confirm the transcriptional regulation of FAD3s by GmWRI14. Our results suggest that FAD3 is indirectly regulated by GmWRI14, representing a new molecular mechanism of fatty acid biosynthesis, in which GmWRI14 regulates LA content in soybean seeds. [ABSTRACT FROM AUTHOR]

Seed yield in soybean is a complex trait and is influenced by multiple genetic and environmental factors. Safflower is one of the oilseeds adapted to a wide range of soil conditions, but micronutrients deficiencies cause a decrease in its grain yield and quality. Hence, an experiment was conducted as a split factorial based on randomized complete block design with three replications at Agricultural and Natural Resources Research Center of Arak, Iran in 2019. Three tillage methods (including no tillage, low tillage and conventional tillage) were considered as the main plots and fertilizer treatments (including 1- Control, 2- Zinc, 3- Iron, 4- Sulfur, 5- Zinc + Iron, 6-Zinc + Sulfur, 7- Iron + Sulfur, and 8- Iron + Sulfur + Zinc) were considered as sub-plots. Results showed that there were significant differences between the fertilizer treatments in terms of seed yield, flower yield, seed weight per head, number of heads, number of seeds per head, percentage of seed protein and seed oil. In the treatment of tillage methods, thousand seed weight and linoleic fatty acid had significant differences. Interaction effects of soil tillage methods X fertilizer were significant on number of heads and linolenic acid. Fertilizer treatment of iron + sulfur + zinc had the highest seed yield (i.e. 1391 kg ha-1 ), flower yield (i.e. 183.7 kg ha-1 ), 1000 seed weight (i.e. 45.8 g), seed weight per head (i.e. 1.27 g), number of heads (i.e. 7.9), protein percentage (i.e. 21.2%) and seed oil percentage (i.e. 26.5). Results of this research showed that application of micronutrient elements by increasing the yield components, particularly 1000-seed weight, number of heads, and the weight of seeds per head led to increases in seed yield, oil percentage and protein percentage of safflower seeds. Though, tillage methods had no significant effect on safflower seed yield. According to the results of this experiment the use of zinc, sulfur, and iron fertilizers along with the method of low tillage can help to increase the grain yield and quality of rapeseed. [ABSTRACT FROM AUTHOR]

Background Significant associations have been reported between serum total nonesterified fatty acid (NEFA) concentrations and coronary heart disease (CHD) mortality and incident nonfatal myocardial infarction (MI) in some prospective cohort studies. Little is known about whether individual or subclasses (saturated, polyunsaturated [n-6 and n-3], and trans fatty acids) of serum NEFAs relate to CHD mortality and nonfatal MI. Methods and Results CHS (Cardiovascular Health Study) participants (N=1681) who had no history of MI, angina, or revascularization or were free of MI at baseline (1996-1997) were included. NEFAs were quantified using gas chromatography. Cox regression analysis was used to evaluate associations of 5 subclasses and individual NEFAs with CHD composite (CHD mortality and nonfatal MI), CHD mortality, and incident nonfatal MI. During a median follow-up of 11.7 years, 266 cases of CHD death and 271 cases of nonfatal MI occurred. In the fully adjusted model, no significant associations were identified between individual NEFA and CHD composite. Exploratory analyses indicated that lauric acid (12:0) was negatively associated (hazard ratio [HR], 0.76; 95% CI, 0.59-0.98; P=0.0328) and dihomo-γ-linolenic acid (20:3n-6) was positively associated with CHD mortality (HR, 1.34; 95% CI, 1.02-1.76; P=0.0351). Elaidic acid (18:1n-7t) was positively associated with incident nonfatal MI (HR, 1.46; 95% CI, 1.01-2.12; P=0.0445). No significant associations were observed for NEFA subclass and any outcomes. Conclusions In CHS participants, 2 NEFAs, dihomo-γ-linolenic and elaidic acids, were positively associated with CHD mortality and nonfatal MI, respectively, suggesting potential susceptibility biomarkers for risks of CHD mortality and nonfatal MI.

α-Linolenic acid (ALA) is an important nutrient component in rapeseed oil, and rapeseed breeders want to either restrain or enhance the function of fatty acid desaturases (FADs) in the ALA biosynthesis pathway. To determine the reason for the upregulation of rapeseed BnFAD genes in two high-ALA accessions, R8Q10 and YH25005, we compared their transcriptome profiles in the seed at 24 days after pollination (DAP) with those of two low-ALA lines, A28 and SW. The expression levels of twenty-eight important genes in the seed samples at 20, 27, and 34 DAP were also investigated using an RT-qPCR. The expression levels of genes involved in flavonoid and proanthocyanidin synthesis, including BnCHS, BnCHI, BnDFR, BnFLS1, BnLDOX, BnBAN, BnTT10, and BnTT12 and genes encoding the transcription factors BnTT1, BnTT2, BnTT8, and BnTT16 were lower in R8Q10 and YH25005 than in A28 and SW. The expression levels of genes encoding master transcription factors in embryo development, such as BnLEC1, BnABI3, BnFUS3, BnL1L, BnAREB3, and BnbZIP67, were elevated significantly in the two high-ALA accessions. Combined with previous results in the Arabidopsis and rapeseed literature, we speculated that the yellow-seededness genes could elevate the activity of BnLEC1, BnABI3, BnFUS3, and BnbZIP67, etc., by reducing the expression levels of several transparent testa homologs, resulting in BnFAD3 and BnFAD7 upregulation and the acceleration of ALA synthesis. Yellow-seededness is a favorable factor to promote ALA synthesis in the two high-ALA accessions with the yellow-seeded trait. These findings provide initial insights into the transcriptomic differences between high-/low-ALA germplasms and a theoretic basis for seed quality breeding.

Soybean accounts for over a quarter of the world's oilseed consumption and over 70% of the world's protein meal consumption. The separate development of high oleic, low linolenic acid (HOLL) soybean and high‐protein (HP) soybean means that no soybean cultivar on the market has an optimal fatty acid profile and increased protein. The objective of this study was to develop and evaluate high protein, high oleic acid, and low linolenic acid (HP‐HOLL) soybean. A five‐gene stack was created using a two‐phase forward breeding scheme and marker‐assisted selection method. Forty‐six HP‐HOLL lines from three genetic backgrounds were grown in six environments in the Southeast United States. Although genotype‐by‐environment interaction was significant for seed composition traits, lines met the >75% and <3% cutoffs for oleic acid and linolenic acid, respectively, and met or exceeded the protein concentration of the HP parent. No negative interaction could be detected between the HP and HOLL traits. Additionally, yield testing in four environments indicated yield parity for some lines, suggesting HP and HOLL soybean cultivars with high yield could be selected. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the microbial oil production by three selected strains, Mortierella isabellina, Cunninghamella echinulata, and Thamnidium elegans, after fermentation of an enzymatic hydrolysate from alkali pre-treated giant reed biomass, in comparison to a semi-synthetic medium, at three increasing nitrogen supplementation levels (0.14, 0.25, and 0.47 g/L). M. isabellina showed the fastest sugar consumption, the highest final cell and oil concentrations (10.9 and 5.6 g/L, respectively), as well as the highest cellular oil content, oil yield/g sugar consumed, and oil productivity (63.4%, 0.19 g/g, and 1 g/L/day, respectively) in the giant reed hydrolysate. The oil yield tended to decrease with an increasing nitrogen content in the cultures. Oleic acid was the most copious fatty acid in the oil for all the fungi. On the contrary, T. elegans exhibited the poorest performances. In particular, M. isabellina showed, respectively, the highest and lowest values of oleic and γ-linolenic acid (52.2 and 3.1%, on average). In comparison, C. echinulata and T. elegans showed much higher γ-linolenic acid content (15.3 and 21.6%, on average). Notably, the C. echinulata cultures showed by far the highest γ-linolenic acid concentration in both substrates (345 and 595 g/L in the giant reed hydrolysate and in the synthetic medium, respectively). Finally, the estimated biodiesel properties of all the oils fell within the limits of the U.S. standards, while the oil of M. isabellina only respected the tighter limits fixed by the E.U. regulations. [ABSTRACT FROM AUTHOR]

Drought stress is one of the most important factors in reducing yield and seed quality of rapeseed. The present study chiefly tries to evaluate the effect of application of brassinosteroid on seed yield, oil content, oil yield, and fatty acids profile of rapeseed genotypes under late-season water deficit stresseen considered. A factorial split-plot test has been conducted in a randomized complete blocks design for two cultivation years (2017-2019) in Karaj, Iran. Experimental treatments include two levels of brassinosteroid (i.e., 0 (control) and 0.1μmol) and two levels of irrigation (full irrigation (control) and withholding irrigation from flowering stage) as factorial in the main plots, and rapeseed genotypes (Nafis, Ahmadi, Okapi, Nima, and Niloufar) as subplots. The levels of oleic acid and linoleic acid in the conditions of application of brassinosteroids have been 0.7% and 11% in full irrigation conditions and 1.1% and 6.4% in withholding irrigation conditions, respectively, compared to the control on the other hand, the use of brassinosteroids in withholding irrigation conditions reduce palmitic acid by 14% compared to the control. Okapi genotype has had the highest and lowest erucic acid and grain yield in irrigation conditions, respectively. Under drought stress conditions, the highest seed yield (3112.3 kg/ha) and the lowest amount of erucic acid (0.26%) belonged to Niloufar genotype. The use of brassinosteroids in both irrigation treatments improve the quality of fatty acids and Niloufar cultivar is recommended in both irrigation conditions [ABSTRACT FROM AUTHOR]

Introduction: Camelina is an oilseed crop that belongs to the Brassicacea family. Camelina is a low- input crop with minimum nutrient requirement and can grow well in low fertility or saline soils when compared to other oil- seed crops like canola, soybean or sunflower. Camelina oil is highly unsaturated and is a rich source of omega-3 fatty acids. The ratio of linoleic acid (LA n-6) to linolenic acid (ALA n-3) in camelina is unique compared to common vegtable oils such as soybean. Camelina oil contains high amounts of tocopherol, which confers a reasonable shelf life without the need for special storage conditions. Generally, crud oils contain many unwanted matters, which must be removed to yield a stable product with a bland or pleasant taste. Therefore, efficient industrial processing involves removing these unpleasant impurities. The processing involves a series of purifying steps, which may be chemical (caustic refining) or physical (bleaching, deodorization). Chemical refining includes degumming, neutralization, bleaching and deodorization as separate process. In this study, the qualitative (FFA and PV) and compositional characteristics of camelina oil obtained from four regins of Ilam, Kermanshah, Hamedan and Fars were investigated after chemical refining operations. Changes in composional specifications were also evaluated before and after the final stage of refining, deodorization. Materials and methods: Oil was extracted from camelina seeds (Soheil cultivar) from above regions by soxhlet method, the extracted oil was degummed with 0.1% phosphoric acid (65-70 ̊C, 15min). Then, it was neutrialized with NaoH (3N) with 10%excess (80 ̊C, 1 min, 1000RPM). The resulting soap was separated from the oil by centrifugation, and the oil was washed twice with distilled water, centrifuged and dried under vacuum at 80 ̊C. Then, the oil was bleached with 0.7% bleaching earth (80-90 ̊C, 30 min) in vacuum and then filtered. The bleached oil was deodorized at 230-240 ̊C for 2.5h at vacuum pressure of – 660 mmHg in a laboratory deodorizer. Fatty acid methyl ester (FAME) of the oils were prepared according to method no: 13126-2 of Iran national standard Organization (INSO). GC analysis of FAME was performed on Young Lin Y.L. 6500 gas chromatography, with a FID, according to INSO no:13126-4 method, separation of fatty acids were carried out on a highly polar RTX 2330 capillary column. The tocopherols were determined according to method no:7211 of the IRAN'S national standard. The HPLC system was used Young Lin Y.L. 9100 equipped with diol column, 25cm* 4mm*5m film thickness. Acetonitril was used as a mobile phase. UV detector was used set as 290nm. FFA and PV were determined according to no:4178, no: 4179. Analyses of fatty acid composition and tocopherol content on bleached oil and deodorized oil were performed and compared. FFA and PV were performed only on deodorized oil. Experiments were performed in three replicate, and data were reported as mean ± standard deviation. The collected data was subjected to a one- way analysis of variance and Duncan's posthoc at the P<0.05. Results and discussion: In fatty acid composition analysis, the most important fatty acid in terms of quantity in all samples was linolenic acid (LAn-3), which ranged from 32.59 to 35.5% in the refined oils and from 34.29 to 35.28% of total fatty acids, in the oils before deodorization, then omega- 6 linoleic acid with 16.49-18.30% of total fatty acids after deodorization, and 16.31-18.47% before deodorization. The trans acid isomers content was not detected more than 0.06% in any of the samples. These was no significant difference in the composition of the above essential fatty acids before and after deodorization, except for the Fars sample. In this oil, the linolenic acid content decreasd significantly after deodorization, which may be due to the difference between the quality of raw materials. Total tocopherol content in the camelina oil samples before deodorization were measured from 893.1-1122mg/kg and after deodorization ranged from 626.68-727.53mg/kg. Significantly loss in tocopherol content (total and individual) was observed after deodorization in all samples (p<0.05). The loss of tocopherol during deodorization may be due to the thermal degradation at high temperature (150-250 ̊C) by oxidation reaction or by chemical reaction, such as the formation of tocopherol esters. The predominantly measured tocopherol isomer was gamma tocopherol. Conclusion: The present study concluded that the chemical refining of camelina oil has no significant effect on the optimal composition of its fatty acids (low content of SFA, high content of α-linolenic acid and optimal pufa n-6 to pufa n-3 ratio), and no trans isomers was formed. FFA and PV after deodorization were in the range of National standard of Iran. The loss of tocopherol can be seen both in total and individual, but its amount in the camelina oil is still significant. [ABSTRACT FROM AUTHOR]

In the study, it was aimed to optimize oil extraction from camelina with ul trasonication at different temperature (30, 45, 60°C), time (15, 30, 45 min) and power (30, 65, 100%) conditions. The oil obtained under optimum conditions was compared with the oil by soxhlet extraction for yield, fatty acid composition and oxidation properties. The ultrasonication optimum conditions were 38.16°C, 15 min, and 30% ultrasonication power, and oil yield was 24.44 g/100g. The peroxide values of the oils obtained by soxhlet and ultrasonication methods were 3.39-69.25 and 2.15-54.19 meqO2/kg oil, respectively. The conjugated diene values of oils obtained by soxhlet and ultrasonication methods were 2.14-9.71 and 1.79-7.22, respectively. Moreover, the applied extraction methods did not cause a significant difference in the fatty acid composition of camelina oil. Ultrasonication technique can be an alternative for the Camelina sativa oil extraction and this oil has potential for using in the functional food production. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the effects of dietary supplementation with biocomplexed selenium and chromium on the fatty acid profile of the breast and thigh of males and females broilers of the Label Rouge lineage. The experimental design was completely randomized arranged in a 3 x 2 factorial scheme corresponding to three diets (control; 0.40 ppm of chromium; 0.50 ppm of selenium) and two sexes. Each treatment consisted of three plots of five broilers. The fatty acid profile was evaluated in the breast and thigh samples. The addition of biocomplexed selenium in the broilers diets increased the content of long chain fatty acid of the omega 3 and 6 series (docosahexaenoic - DHA and arachidonic acids, respectively), the total of omega 6 and the stearic acid content on the breast (pectoralis major muscle). In addition, selenium supplementation caused a reduction in oleic acid content and in total of monounsaturated in the same muscle. Males had higher contents of linoleic and linolenic acids, and total of polyunsaturated; and lower content of oleic acid, total of monounsaturated and saturated in the thigh. The broilers supplemented with biocomplexed selenium and males presented better lipid quality in the muscle by breast and thigh, respectively. [ABSTRACT FROM AUTHOR]

Context: Feeding dairy cows with plant oils rich in unsaturated fatty acids is a recommended strategy for increasing the proportion of polyunsaturated fatty acids in milk for human consumption. Aims: We evaluated the effects of supplementation with peanut oil (high oleic acid content) and sunflower oil (high linoleic acid content) on the fatty acid profile and oxidative stability of milk and the expression of oxidative stress response, lipogenic-related and insulin-like growth factor 1 (IGF-1) genes in pasture-fed Jersey cows in early lactation. Methods: Twenty-four cows were distributed in a randomised block design. Treatments were four dietary supplements of concentrate alone, or concentrate plus oil (59.6 g/kg dry matter): peanut oil, sunflower oil, or a 1:1 (v/v) mixture of peanut and sunflower oils. Analyses were performed at 30 and 60 days in milk. Key results: Both peanut oil and mixed oil diets increased the hypocholesterolemic/hypercholesterolemic ratio (P < 0.001) and health-promoting index (P < 0.001) of milk. The sunflower oil diet increased the thrombogenicity index (P = 0.005) and conjugated diene production (P < 0.001). mRNA abundance of fatty acid synthase (P = 0.003), acetyl-CoA carboxylase alpha (P = 0.001), and stearoyl-CoA desaturase (P < 0.001) decreased with the sunflower oil diet. mRNA abundance of superoxide dismutase decreased (P = 0.01) with sunflower oil and mixed oil diets. The control diet tended (P = 0.08) to reduce IGF-1 mRNA abundance. Conclusions and implications: Supplementation with peanut oil increases the health-promoting index of milk, whereas supplementation with sunflower oil increases susceptibility to lipid peroxidation. Supplementation with sunflower oil reduces the expression of lipogenic and antioxidant enzyme genes, and supplementation with unsaturated fats may reduce IGF-1 gene expression in early-lactation cows. The relatively small sample size means that more studies are needed. We evaluated the effects of supplementation with peanut and sunflower oils on fatty acid profile, milk oxidative stability, and expression of oxidative stress response genes in pasture-fed Jersey cows. Peanut oil supplementation increased the health-promoting index of milk, whereas sunflower oil supplementation increased susceptibility to lipid peroxidation. [ABSTRACT FROM AUTHOR]

Acetylcholinesterase (AChE, E.C. 3.1.1.7) termed as the true cholinesterase functions to end cholinergic transmission at synapses. Due to its diverse expression in non-neural tissues such as erythrocytes and bones along with its various molecular forms, researchers seek a non-classical role for this protein. Here, the inhibitory action of unsaturated 18 carbon fatty acids linoleic acid and alpha-linolenic acid and 20 carbon fatty acid arachidonic acid on AChE were investigated. Enzyme activity was measured in kinetic assay method according to Ellman assay utilizing acetylthiocholine. Analysis of the activity data revealed that among the fatty acids examined the IC50 values differed according to the length of the fatty acid and the number of the double bonds. Arachidonic acid, a 20-carbon fatty acid with 4 unsaturated bonds (20:4 n-6, cis 5,8,11,14) displayed an IC50 value of 2.78 µM and Ki value of 396.35 µM. Linoleic acid, an essential 18-carbon fatty acid (18:2 n-6, cis 9,12) had an IC50 value of 7.95 µM and Ki value of 8027.55 µM. The IC50 value of alpha-linolenic acid, 18-carbon fatty acid (18:3 n-3, cis-9,12,15) was found as 179.11 µM. Analysis of the data fit the inhibition mechanism for linoleic, alpha-linolenic and arachidonic acid as mixed-type; non-competitive. Molecular docking complied with these results yielding the best score for arachidonic acid. The alkenyl chain of the fatty acids predictably reached to the catalytic site while the carboxylate strongly interacted with the peripheric anionic site. [ABSTRACT FROM AUTHOR]

Maternal food habits and gut microbiota composition have potential effects on fetal neurodevelopment, impacting Autism Spectrum Disorder (ASD). Our research aims to outline the relationship that ingestion of polyunsaturated fatty acids (PUFAs) and the composition of maternal gut microbiota have with the possible development of ASD in offspring. We suggest that genetic factors could be related to the different conversions between unsaturated fatty acids according to sex and, mainly, the impact of the pregnancy diet on the higher or lower risk of neurological impairments. The proportion of the phyla Firmicutes/Bacteroidetes is high with an increased consumption of linoleic acid (LA, n-6 PUFA), which is associated with maternal intestinal dysbiosis and consequently starts the inflammatory process, harming myelinization. In contrast, the consumption of α-linolenic acid (ALA, n-3 PUFA) tends to re-establish the balance of the maternal microbiota with anti-inflammatory action. Moreover, human observational studies showed a strong correlation between the consumption of n-3 PUFA, mainly above 340 g of fish per week, with beneficial effects on infant neurodevelopment. Therefore, we suggest that the proper intake of foods rich in n-3 PUFAs and their supplementation during pregnancy until lactation has an impact on reducing the development of ASD. Controlled studies with n-3 PUFA supplementation are still necessary to verify the ideal dose and the best form of administration. [ABSTRACT FROM AUTHOR]