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Subsoiling practice is an essential tillage practice in modern agriculture. Tillage forces and energy consumption during subsoiling are extremely high, which reduces the economic benefits of subsoiling technology. In this paper, a cicada-inspired biomimetic subsoiling tool (CIST) was designed to reduce the draught force during subsoiling. A soil–tool interaction model was developed using EDEM and validated using lab soil bin tests with sandy loam soil. The validated model was used to optimize the CIST and evaluate its performance by comparing it with a conventional chisel subsoiling tool (CCST) at various working depths (250–350 mm) and speeds (0.5–2.5 ms−1). Results showed that both simulated draught force and soil disturbance behaviors agreed well with those from lab soil bin tests, as indicated by relative errors of

Subsoiling is an essential practice in conservation tillage technology. The amount of disturbed soil at various depths resulting from subsoilers with different parameters has an important effect on soil properties (e.g., bulk density and water infiltration). The information regarding the effects of subsoiling on the characteristics of soil water infiltration is essential for the design of subsoiling tools. In this study, the effects of the wing mounting height (h) (75–155 mm) of the subsoiler on soil disturbance and soil water infiltration were modelled using HYDRUS-2D and validated using field experiments. Results showed that reducing h values resulted in larger soil disturbance area ratios, soil water infiltration rates (f(t)), distances of vertical wetting front movement (DVWs), accumulative infiltrations (AINs), and soil moisture contents at depths of 10–30 cm. The relationships among characteristics of soil water infiltration, h and time (t), were developed. The stable infiltration rates (fs) varied quadratically with h and the corresponding coefficient of determination (R2) was 0.9869. The Horton model is more suitable for describing the relationship between f(t) and t under the tested soil conditions, as compared with the Kostiakov and Philip models. According to the results of soil water content at different depths from the HYDRUS simulations and field experiments, the developed soil water infiltration model had a good accuracy, as indicated by RMSEs of 2 values of >0.95, and mean relative errors of

At present, the measurement of tillage depth is mainly based on manual measurement, but the manual raking method results in low measurement accuracy and high labor intensity. Due to the complexity of soil, theoretical research on tillage depth is relatively scarce. In order to provide a new research direction and research idea for soil stratification, topsoil was taken as the research object of this paper. The correlation between penetration resistance and penetration depth of a probe in a cultivated soil was studied, and a mathematical model was established. There is a certain similarity between the process of spherical cavity expansion and the process of probe penetration, so we introduced the theory of spherical cavity expansion into the modeling of penetration resistance of the cultivated soil. In this paper, the spherical cavity expansion theory of unsaturated soil was used as the basis for solving the penetration resistance. And the unified strength criterion was employed as a yield condition of the soil to set a stress solution and a displacement solutionin into of the probe penetrating into the elasto-plastic zone of the cultivated soil to determine the model of expansion force. We have carried out indoor tests to revise the expansion force model. Firstly, according to the range of soil density and water content in the field, the soil densities were classified into 1.1×103kg/m3, 1.2×103kg/m3 and 1.3×103kg/m3, and the water contents were divided into 10%, 15% and 20%. In addition, the orthogonal tests were performed at different levels. The soil was put into the barrel, and the probe was inserted into the soil in the barrel at the speed of 8mm/s to determine the test values of the change of the probe penetration resistance with depth. Finally, the expansion force model was fitted with the results of the indoor test, and coefficient B was introduced to express the influence degree of density and water content on the resistance. Coefficient B was substituted into the expansion force model to obtain the penetration resistance model of the cultivated soil. Through the goodness of fit analysis of the penetration resistance model, the results show that the overall average goodness of fit of the penetration resistance modelat was up to 0.871 at different water contents and densities, which was a good fit and could present novel insights into the study relating to soil stratification theory.

Staphylococcus aureus (S. aureus) is an extremely halotolerant pathogenic bacterium with high osmotic stress tolerance, and it is frequently encountered in aquatic production and preservation. However, the mechanism underlying the extremely high osmotic stress tolerance of S. aureus remains unclear. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) method was used to identify the differentially expressed proteins (DEPs) under different sodium chloride (NaCl) concentrations. Compared with the control group (0% NaCl), the 10 and 20% NaCl groups had 484 DEPs and 750 DEPs, respectively. Compared with the 10% NaCl group, the 20% NaCl group had 361 DEPs. Among the DEPs, proteins involved in fatty acid synthesis, proline/glycine betaine biosynthesis and transportation, stress tolerance, cell wall biosynthesis and the TCA cycle were upregulated, whereas proteins associated with biofilm formation and pathogenic infections were downregulated. The results obtained in this study indicate that under extremely high osmotic stress, modification of the cell membrane structure, increased biosynthesis and transportation of osmotic protectants, and redistribution of energy metabolism contribute to the osmotic stress tolerance of S. aureus, and the infectious ability of the bacteria may be limited. The aim of this study was to provide new insight into how S. aureus tolerates the high-salt conditions involved in aquatic production and preservation.

Objective: a new method for testing the performance index of the impurity removal rate of Chinese cabbage seeds cleaning machine is studied, so as to improve the cleaning efficiency of Chinese cabbage seeds. Methods: According to the cleaning process of Chinese cabbage seeds (pre-cleaning, basic cleaning, selection and size grading), the performance index of the impurity removal rate of cleaning machine at each stage is determined. According to the dynamic analysis results of Chinese cabbage seeds on the screen surface of the cleaning machine, the working parameters of the cleaning machine are determined, and the cleaning machine for Chinese cabbage seeds is carried out. The rationality of impurity removal rate is checked. Results: The optimum combination of working indexes of Chinese cabbage seed cleaning machine is: crank radius of 15.5 mm, crank speed of 320 r/min, vibration direction angle of 2.7° and screen inclination angle of 5°. When the impurity removal rate of Chinese cabbage seeds is 83%, the frequency of the wind turbine of the cleaning machine is 36 Hz, the opening of the air door is 80%, and the Chinese cabbage seeds are selected. The suitable cleaning voltage of the seed is 1000 V. Conclusion: The proposed method can comprehensively and accurately detect the performance indexes of impurity removal rate of Chinese cabbage seed cleaning machine, and improve the efficiency of Chinese cabbage seed cleaning. [ABSTRACT FROM AUTHOR]