1. Expression of the gene for tear lipocalin/von Ebner's gland protein in human prostate
- Author
-
Petra Merschak, Bernhard Redl, Helmut Klocker, Paul Holzfeind, Zoran Culig, Hans Feichtinger, and Hermann Rogatsch
- Subjects
Male ,Transcription, Genetic ,Lingual von Ebner's gland protein ,Biochemistry ,Polymerase Chain Reaction ,law.invention ,Structural Biology ,Prostate ,law ,Testis ,Cloning, Molecular ,Intestinal Mucosa ,Northern analysis ,Recombinant Proteins ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,Organ Specificity ,Recombinant DNA ,Female ,medicine.medical_specialty ,DNA, Complementary ,Sequence analysis ,Molecular Sequence Data ,RT-PCR ,Biophysics ,Thymus Gland ,Biology ,Complementary DNA ,Internal medicine ,Genetics ,medicine ,Escherichia coli ,Humans ,Amino Acid Sequence ,RNA, Messenger ,Salivary Proteins and Peptides ,Molecular Biology ,Gene ,Lipocalin 1 ,DNA Primers ,Base Sequence ,Ovary ,Cell Biology ,Blotting, Northern ,Molecular biology ,Reverse transcriptase ,Endocrinology ,Tear lipocalin ,Carrier Proteins ,recombinant protein ,Spleen - Abstract
Northern analysis of human multiple tissue blots containing poly A+ RNA from spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes revealed that a prostate specific transcript hybridizes to a tear lipocalin/von Ebner's gland protein (TL/VEGP) gene probe. To characterize this transcript, the corresponding cDNA was amplified by reverse transcription (RT)-PCR. Cloning and sequence analysis showed that it was identical to the tear lipocalin cDNA isolated from human lachrymal glands. Immunohistochemical analysis on thin layer sections of human prostate using a tear lipocalin specific antiserum confirmed the expression of this cDNA in prostate. Thus, our results clearly argue against a unique function of TL/VEGP in human tear fluid or saliva. The human cDNA was expressed in E. coli using the pQE system yielding a recombinant protein which shows biochemical properties identical to the native TL/VEGP.
- Published
- 1996