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Sweet potato is a crop which is drought-resistant, tolerant of poor soil, and owns increasingly enormous potential. It is an important foodstuff, a raw material for starch industry, a kind of health food to prevent cancer and a new raw material for energy as well. The sweet potato planting area in China per year on average is usually 3 667 000 hectares, accounting for 45% of the world's planting area. As sweet potato vines are very long and broomy, the vines must be cleaned up before sweet potatoes are harvested, otherwise are unable to be harvested. Vine crushing and returning machine is usually the dominant form adopted to clean sweet potato vines internationally, but the problems existed in this type of machine were obvious such as low smashing proportion and more stubble residue on the top ridge. New problems have been brought by long stubble such as blocking, larger load and worse smooth working property. Aiming at these problems, the machinery and agronomy should be combined, in order to prove mechanical properties of sweet potato vine and stalk during harvest and working quality of sweet potato vine crushing and returning testing machine. In this paper, the types of fresh purple sweet potato Ningzi 1 and Ningzi 2 in Nanjing, Jiangsu Province were regarded as the tested subject, the electronic universal testing machine, electric drying oven with forced convection, and sweet potato vine crushing and returning testing machine etc. were applied, and the change of moisture percentage and shear strength of sweet potato stalk during harvest were studied. The results showed that at the beginning of experience harvest period, when the percentage of moisture were 81.5% and 78.1%, the shear force were 90.1 and 94.8 N, respectively, during 18 testing days the moisture percentage of sweet potato stalk reduced by 14.8% and 13.97%, respectively, but the shear force of stalk increased by 36.3 and 37.5 N respectively, which showed that the moisture percentage of sweet potato stalk was lower and the shear force was larger as the growth time of plants went on. The internal relations between mechanical crushing quality and percentage of moisture, shear strength were revealed: during the testing period of 18 days, the qualified rates of vine's crushing length for sweet potato type Ningzi 1 and Ningzi 2 dropped by 6.3% and 6.7% respectively, stubble length on top ridge increased by 3.6 and 4.4 cm respectively, which showed that with the increasing of the growth period and the rising of stalk's shear force, the working quality of sweet potato vine crushing and returning machine declined. The quadratic function regression equations on the change between shearing force and moisture percentage of the two types of fresh purple sweet potatoes' stalk were obtained with Matlab when the percentage of moisture was 86.92%-70.08%, and the fitting coefficient of determination R² both reached above 0.99. From the point of increasing the quality of mechanical smashing and mechanical work, it was put forward that the most suitable time of mechanical crushing for the two kinds of purple sweet potatoes during harvest was about 6-8 days, which was a relatively short time. The results offer the direct basis for parameter design of new sweet potato vine crushing and returning testing machine such as machine speed, cutter linear velocity, and also provide the important reference for the selection of suitable harvest period and cultivation agronomic research that fits for machinery operations. [ABSTRACT FROM AUTHOR]

Myocardial ischemia-reperfusion injury (MIRI) has been confirmed to induce endoplasmic reticulum stress (ERS) during downstream cascade reactions after the sufficient deterioration of cardiomyocyte function. However, clinically outcomes have been inconsistent with experimental findings because the mechanism has not been entirely elucidated. Dexmedetomidine (DEX), an α 2 adrenergic receptor agonist with anti-inflammatory and organ-protective activity, has been shown to attenuate IRI in the heart. The present study aimed to determine whether DEX is able to protect injured cardiomyocytes under in vitro hypoxia/reoxygenation (H/R) conditions and evaluate the conditions under which ERS is efficiently ameliorated. The cytotoxicity of DEX in H9c2 cells was evaluated 24 h after treatment with several different concentrations of DEX. The most appropriate H/R model parameters were determined by the assessment of cell viability and injury with Cell Counting Kit-8 and lactate dehydrogenase (LDH) release assays after incubation under hypoxic conditions for 3 h and reoxygenation conditions for 3, 6, 12 and 24 h. Additionally, the aforementioned methods were used to assess cardiomyocytes cultured with various concentrations of DEX under H/R conditions. Furthermore, the degree of apoptosis and the mRNA and protein expression levels of glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP) and caspase-12 were evaluated in all groups. The addition of 1, 5 and 10 µM DEX to the cell culture significantly increased the proliferation of H9c2 cells by >80% under normal culture conditions. In the H/R model assessment, following 3 h of anoxia exposure, H9c2 cell viability decreased to 62.67% with 3 h of reoxygenation and to 36% with 6 h of reoxygenation compared with the control. The viability of H9c2 cells subjected to hypoxia for 3 h and reoxygenation for 3 h increased by 61.3% when pretreated with 1 µM DEX, and the LDH concentration in the supernatant was effectively decreased by 13.7%. H/R significantly increased the percentage of apoptotic cells, as detected by flow cytometry, and increased the expression levels of GRP78, CHOP and caspase-12, while treatment with either DEX or 4-phenylbutyric acid (4-PBA) significantly attenuated these effects. Additionally, despite the protective effect of DEX against H/R injury, 4-PBA attenuated the changes induced by DEX and H/R. In conclusion, treatment with 1 µM DEX alleviated cell injury, apoptosis and the increases in GRP78, CHOP and caspase-12 expression levels in H9c2 cells induced by 3 h of hypoxia and 3 h of reoxygenation., (Copyright: © Zhu et al.)