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Background: Eurycoma longifolia Jack (Simaroubaceae) root extracts and Persicaria minor Huds. (Polygonaceae) leaf extracts are known to exhibit antioxidant activity, while their antihyperuricemic effects remain unclear. Objective: The objective of this study is to investigate the anti-hyperuricemic activity of Biokesum® (extract of P. minor) and Physta® (extract of E. longifolia). Methods: In vitro anti-hyperuricemic activity for Biokesum® and Physta® was measured by xanthine oxidase inhibition based on a biochemical enzymatic assay at concentrations of 1, 10, and 100 μg/mL. In vivo anti-hyperuricemic activity was assessed in potassium oxonate-induced hyperuricemia Sprague-Dawley rat model. Biokesum® at doses of 100, 300, and 500 mg/kg and Physta® at doses of 300 and 500 mg/kg were administered for 7 days to the hyperuricemic rats. Serum uric acid was measured on days 0, 1, 3, and 7, and liver xanthine oxidase activity was measured on day 8. Allopurinol was used as positive control in both in vitro and in vivo investigations. Results: Based on xanthine oxidase biochemical enzymatic assay, IC50 of Biokesum® and Physta® were 88.6 μg/mL and > 100 μg/mL respectively. In hyperuricemic rats, Biokesum® and Physta® significantly reduced the serum uric acid by 5.6% and 5.5% respectively at 300 mg/kg (p < 0.05) and 9.8% and 9.0% respectively at 500mg/kg (p < 0.001) compared to untreated rats. Significant liver xanthine oxidase inhibition at 37.5% and 34.1% were observed in rats treated with Biokesum® and Physta® respectively. Conclusion: The study has demonstrated hyperuricemic activity of Biokesum® and Physta®, clinical studies in hyperuricemia-related diseases population are required to further confirm the activity.

Bin-Bin Jin, Yan Ma, Xiu-Hua Zhao, Yi-Ling Teng, Shang-Yong Zhu Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of ChinaCorrespondence: Shang-Yong Zhu, Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, 530021, People’s Republic of China, Email zhushangyong@sina.comObjective: To analyze the risk factors of the operators on contrast agent intravasation during hysterosalpingo-contrast sonography (HyCoSy).Methods: We retrospectively collected 399 infertile women who underwent HyCoSy by the same sonographer. These patients were divided into two groups according to the way how the assistants connected the syringe to the uterus radiography catheter to inject the contrast agent. We analyzed whether the use of different contrast bolus injection methods had any influence on the incidence of intravasation during HyCoSy.Results: There was no significant difference between the two groups with different cross-sectional areas of the syringe outflow tract in the risk variables for intravasation, but the intravasation rates of the two groups were different, 26.4% in group A and 17.1% in group B, P < 0.05. The primary manifestation was that when both fallopian tubes were unobstructed, the intravasation rate of group B with smaller cross-sectional area of the outflow tract of the syringe was lower, and the difference was statistically significant. The inferences drawn from our physics model were also in line with the clinical results.Conclusion: The influence of different operators on the contrast agent intravasation rate of HyCoSy cannot be ignored. The assistants of HyCoSy examination should inject the contrast agent slowly and steady, and a needle can be used as a flow restrictor to control the flow into the uterine cavity per unit time, slow down the rising speed of intrauterine pressure, and avoid the accumulation of contrast agent in the uterine cavity, so as to reduce the intravasation caused by operator factors.Keywords: intravasation, hysterosalpingo-contrast sonography, ultrasonography, contrast agent, fallopian tube patency, infertility

The clinical application of complex molecular classifiers as diagnostic or prognostic tools has been limited by the time and cost needed to apply them to patients. Using an existing 50-gene expression signature known to separate two molecular subtypes of the pediatric cancer rhabdomyosarcoma, we show that an exhaustive iterative search algorithm can distill this complex classifier down to two or three features with equal discrimination. We validated the two-gene signatures using three separate and distinct datasets, including one that uses degraded RNA extracted from formalin-fixed, paraffin-embedded material. Finally, to show the generalizability of our algorithm, we applied it to a lung cancer dataset to find minimal gene signatures that can distinguish survival. Our approach can easily be generalized and coupled to existing technical platforms to facilitate the discovery of simplified signatures that are ready for routine clinical use. Cancer Res; 73(18); 5625–32. ©2013 AACR.

PDF file - 714K, Supplementary Figure 1 Two-gene combinations from the 50-gene F1/F2 classifier with >98% classification efficiency in the Williamson data set. Supplementary Figure 2 Thirteen two-gene combinations with >99% classification efficiency, identified from exhaustive analysis of top 2000 genes. Supplementary Figure 3 Gene dyads with classification efficiency >95% identified through exhaustive search of all possible combinations. Supplementary Figure 4 Detection of gene expression is comparable for fresh-frozen and paraformaldehyde-fixed material with nCounter. Supplementary Figure 5 Gene expression detected in each case with the Williamson F1/F2 metagene nCounter Code set. Supplementary Table 1 Four-gene combinations with ≥ 99% accuracy. Supplementary Table 2 Dyad classifiers from T-test ranking of Williamson data set with ≥ 98% accuracy, applied to Davicioni data. Supplementary Table 3 T-test dyad classifier genes found in F1/F2 metagenes (L) and F1/F2 metagene probes excluded from dyad pairs (R). Supplementary Table 4 Top-scoring classifiers from exhaustive search of 1.5 billion gene pairs from Williamson data set, also applied to Davicioni and Exon data. Supplementary Table 5 Tumor sample characteristics. Supplementary Table 6 Genes in nCounter set. Supplementary Table 7 PCA Scores by tumor sample for nCounter Data. Supplementary Table 8 CA Loadings for nCounter Data. Supplementary Table 9 Top-scoring nCounter dyad classifiers. Supplementary Table 10 Top gene combinations - Lung cancer data

Introduction: Mobile health (mHealth) lifestyle interventions can help mitigate cardiometabolic disease. However, Hispanic adults are underrepresented in these interventions, report lower physical activity (PA) levels and are disproportionately affected by cardiometabolic disease compared to non-Hispanic White (NHW) adults. We conducted a secondary analysis of the effects of an 8-week mHealth exercise intervention on exercise behavior in Hispanic and NHW adults. Hypothesis: A remotely delivered mHealth exercise program involving a wearable sensor, mobile application and wellness education would promote similar exercise behavior in both Hispanic and NHW adults. Methods: Thirty-eight Hispanic and 21 NHW sedentary (< 60 min/week of exercise) adults were randomized (1:1:1) to 8 weeks of increasing behavioral resources: Level 1 (wellness education, n=16), Level 2 (wellness education + pre-recorded exercise videos, n=24), or Level 3 (wellness education + livestream group exercise, n=19). Participants were given a chest strap sensor (Myzone MZ-3) to use during exercise and a mobile app to interact with other participants. A weekly email delivered wellness education, feedback and PA encouragement. Level 2 (videos) and Level 3 (classes) performed 35-min 3x/wk of instructor-led, high-intensity functional training designed to improve cardiometabolic risk factors. Sensor use (3x/wk encouraged) and time spent in moderate-to-vigorous exercise effort (MVET) were captured by the MZ-3. MVET was calculated as the time spent in effort >64% maximum heart rate. Baseline and post-intervention PA were captured using the International Physical Activity Questionnaire. Differences between groups were tested using univariate ANOVA with Bonferroni-adjusted t tests in post hoc analyses. Results: Hispanic participants were younger (30.6 ± 10.1 yr, mean ± SD) and performed less PA (22.6 ± 70.2 min/wk) at baseline compared to NHW (39.7 ± 16.8 yr, 90.1± 90.2 min/wk; p0.05), or in weekly sensor use during the intervention (Hispanic 1.7 ± 1.8 sessions/wk, NHW 2.4 ± 1.6 sessions/wk, p>0.05). Self-reported PA increased from baseline to post-intervention in both groups (p Conclusion: An 8-week mHealth lifestyle program increased PA behavior in sedentary participants. However, Hispanic participants exercised at lower intensities than non-Hispanic White participants. Future work is needed to identify barriers in meeting MVPA recommendations in minority populations so that culturally tailored interventions can be developed.

Metastatic renal carcinoma is a kind of tumor with high degree of malignancy, but there are no effective treatment methods and strategies at present. In this study, we designed a folate-grafted PEI600-CyD (H1) nanoparticle-mediated DNA vaccine containing an adjuvant of high mobility group box 1 protein (HMGB1) and a tumor-specific antigen of B7H3 (CD276) for renal carcinoma therapy. Mice bearing subcutaneous human B7H3 (hB7H3)-Renca tumor were immunized with H1-pHMGB1/pB7H3, H1-pB7H3, H1-pHMGB1, or Mock vaccine. Compared to other control groups, the growth of the tumor was significantly inhibited in H1-pHMGB1/pB7H3 vaccine group. The increased proportion and mature of CD11c+ DCs were observed in the spleen of H1-pHMGB1/pB7H3 treated mice. Likewise, HMGB1 promoted B7H3 vaccine to induce tumor-specific CD8+ T cell proliferation and CTL responses. Beyond that, H1-pHMGB1/pB7H3 vaccine strengthened the induction of functional CD8+ T cells. With the depletion of CD8+ T cells, the anti-tumor effect of H1-pHMGB1/pB7H3 also disappeared, indicating that CD8+ T cells are the key factor of the anti-tumor activity of the vaccine. So, to sum up, H1-pHMGB1/pB7H3 vaccine could achieve the desired anti-tumor effect by enhancing the response of tumor-specific functional CD8+ T cell responses. H1 nanoparticle-based vaccines may have great potential and prospect in the treatment of primary solid tumors.

Background Diminished gluteus maximus muscle strength has been proposed to be contributory to various lower-limb injuries. As such, it is of clinical importance to perform hip extensor strength testing in a position that biases torque contribution of the gluteus maximus relative to the other hip extensors (i.e. hamstrings). Objectives To determine the relative torque contributions of the gluteus maximus and hamstring muscles in various hip extensor strength testing positions. Methods 13 Young, healthy participants performed maximum isometric hip extension on a dynamometer in 4 different positions that varied in terms of hip and knee flexion. Surface electromyography (EMG) was used to assess activation of gluteus maximus and hamstrings during the maximum isometric contractions. Normalized EMG data were used as an input to determine individual muscle contribution to hip extension torque production using SIMM modeling software. The gluteus maximus/hamstring torque contribution ratio was compared across the 4 positions using a one-way repeated-measures ANOVA. Results The highest gluteus maximus torque contribution value occurred in positions where the hip was flexed to 45°, while the highest hamstring torque contribution occurred in positions in which the knee was fully extended. The gluteus maximus/hamstring torque contribution ratio was highest at 0° of hip extension and 90° of knee flexion. Conclusion Testing isometric hip extensor strength at 0° of hip extension and 90° of knee flexion should be considered in order to bias torque production of the gluteus maximus relative to the hamstrings.

Objective In this study, we trace the changes in the clinical and histological pattern of IgA nephritis (IgAN) in Singapore as it has evolved over 4 decades and compare the clinical, demographic, histological, and renal outcome of patients with IgAN from the 1st decade and the 4th decade. Materials and methods This is a retrospective study of all histologically proven IgAN diagnosed between 1976 and 2018. Clinical, laboratory, and histological characteristics between the 1st and the 4th decade, including treatment which could influence the disease progression and renal outcome of these two groups, were compared. We used the Oxford classification to compare the renal biopsy changes for these 2 decades as we were able to retrieve 125 renal biopsy tissues for the 1st cohort of IgAN studied in the 1970s for the comparative study. Results The commonest clinical presentation throughout the first 3 decades was asymptomatic hematuria and proteinuria (63, 52, and 49%, respectively). In the 4th decade, nephrotic syndrome (31%) was the commonest followed by asymptomatic hematuria and proteinuria (30%), hypertension (21%), and chronic renal failure (11%). The data showed that treatment can modify the Oxford MEST - Crescent scores. Renin-angiotensin system (RAS) blockers modified the S scores, immunosuppressants modified the T and C scores, and combination therapy with RAS blockers and immunosuppressants modified the E, S, and T scores. Conclusion The Oxford MEST classification offers a robust and expressive classification for early and late disease progression with respect to the development of end-stage renal disease (ESRD). E and S seem to be indices of continuing disease activity with progressive glomerulosclerosis, probably still amenable to therapy, but T was a predictive indicator for those destined for ESRD and no longer amenable to therapy.

The traditional methods to prepare electrodes of lithium-ion batteries need to add binders which reduce the capacity of electrode materials. In this work, CoO nanosheets/CoO film as self-supported anodes were designed and prepared. The CoO nanosheets/CoO film exhibited better electrochemical performance and structural stability than CoO nanosheets directly grown on copper foil and most previous CoO-based electrode materials. The CoO film prepared by magnetron sputtering firmly fixed the nanosheets prepared by the hydrothermal method on the film. The buffer layer of CoO film can prevent CoO nanosheets from crushing and falling off, thus greatly improving the electrochemical performance of this kind of material. The CoO nanosheets/CoO film has great potential applications in lithium-ion batteries.

Background: We recently developed the Coronary Artery Tree description and Lesion EvaluaTion (CatLet) angiographic scoring system. Our preliminary study demonstrated that the CatLet score better predicted clinical outcomes than the SYNTAX score. The current study aimed at assessing whether 3 clinical variables (CVs) – age, serum creatinine, and left ventricular ejection fraction (LVEF) – improved the performance of the CatLet score in outcome predictions in patients with acute myocardial infarction (AMI). Methods: This study was a post hoc study of the CatLet score validation trial. Primary endpoint was major adverse cardiac or cerebrovascular events (MACCEs), and secondary endpoints were all-cause deaths and cardiac deaths. Results: Over 1,185 person-years (median [interquartile range], 4.3 [3.8–4.9] years), there were 64 MACCEs (20.8%), 56 all-cause deaths (18.2%), and 47 cardiac deaths (15.2%). The addition of the 3 CVs to the stand-alone CatLet score significantly increased the Harrell’s C-index by 0.0967 (p = 0.002) in MACCEs, by 0.1354 (p < 0.001) in all-cause deaths, and by 0.1187 (p = 0.001) in cardiac deaths. When compared with the stand-alone CatLet score, improved discrimination and better calibration led to a significantly refined risk stratification, particularly at the intermediate-risk category. Conclusions: CatLet score had a predicting value for clinical outcome in AMI patients. This predicting value can be improved through a combination with age, serum creatinine, and LVEF (http://www.chictr.org.cn; unique identifier: ChiCTR-POC-17013536).

Amomum longiligulare polysaccharide 1 (ALP1) was extracted from Amomum longiligulare T.L. Wu fruits and the poly (lactic-co-glycolic acid) nanoparticle enveloping ALP1 (ALPP) showed a good promoting effect on the activation of macrophages in our previous study. To further understand the immunomodulatory property of ALPP, the effect of ALPP on T lymphocytes and dendritic cells was investigated in the present study. The proliferation rates of chicken T lymphocytes and chicken bone marrow dendritic cells (chBM-DCs) that were treated with ALP1 or ALPP were determined by using MTT method. Meanwhile, the relative mRNA levels of cytokines from T lymphocytes and surface molecules of chBM-DCs were determined by using qRT-PCR method. In addition, the drug uptake capacity of chBM-DCs was also tested. As a result, the promoting effect on the proliferation of T lymphocytes and the Th1-type immune response of ALPP was better than that of ALP1. In addition, ALPP was much more effectively swallowed by chBM-DCs so that its promoting effect on the proliferation and maturation of chBM-DCs was higher than that of ALP1. To conclude, ALPP had a stronger immunomodulatory activity than ALP1, and showed the potential to become a new type of immune booster.

Radiographers provide mobile radiography services for patients who are critically ill as well as patients isolated due to highly infectious diseases such as COVID-19. The pandemic has caused the demand for mobile radiography to increase. This study aims to understand the experience of radiographers performing mobile radiography during the COVID-19 pandemic to identify the success criteria and challenges faced.This study utilized a cross sectional online survey to obtain data. The online survey was disseminated to radiographers working in public hospitals who have performed mobile radiography from February 2020 to September 2021. The key sections explored in the survey are: (1) demographics, (2) operations, (3) adequacy of resources, and (4) success criteria. The answers were obtained in the form of multiple choice questions, Likert scales or free text.Radiographers reported a rise in mobile radiography workload as well as increased time required to perform an examination for COVID-19 patients. The factors identified for success criteria were: (1) infection control management, (2) resource management (3) modified techniques and (4) improved workflow. The challenges encountered were: (1) nature of exam, (2) juggling the demand for mobile imaging and (3) staff well-being.As the COVID-19 situation is evolving, departments have to constantly refine policies and processes as well as ensure the provision of adequate resources such as manpower and personal protective equipment (PPE) so that radiographers feel supported and can perform their duties safely.This study has identified challenges that radiographers face in mobile radiography as well as the success criteria that can aid radiographers in their job.

Objective Tuberculosis is the leading cause of death from a single infectious agent. The emergence of antimicrobial resistant Mycobacterium tuberculosis strains makes the problem more severe. Pyrazinamide (PZA) is an important component for short-course treatment regimens and first- and second-line treatment regimens. This research aims for fast diagnosis of M. tuberculosis resistance to PZA and identification of genetic features causing resistance. Materials and Methods We use clinically collected genomic data of M. tuberculosis that are resistant or susceptible to PZA. A machine learning platform is built to diagnose PZA resistance using the whole genome sequence data, and to identify resistance genes and mutations. The platform consists of a deep convolutional neural network (DCNN) model for resistance diagnosis and a support vector machine (SVM) model as a surrogate to identify resistance genes and mutations. Results The DCNN model achieves a PZA resistance diagnosis accuracy of 93%. Each prediction takes less than a second. The SVM has revealed 2 novel genes, embB and gyrA, besides the well-known pncA gene, and 9 mutations that harbor PZA resistance. Discussion The DCNN and SVM machine learning platform, if used together with the real-time genome sequencing machines, could allow for rapid PZA diagnosis, allowing for critical time to ensure good patient outcomes, and preventing outbreaks of deadly infections. Furthermore, identifying pertinent resistance genes and mutations will help researchers better understand the biological mechanisms behind resistance. Conclusions Machine learning can be used to achieve high-accuracy resistance prediction, and identify genes and mutations causing the resistance.

Background:. Previously, we developed a novel Coronary Artery Tree description and Lesion EvaluaTion (CatLet©) angiographic scoring system, which was capable of accounting for the variability in the coronary anatomy and assisting in the risk-stratification of patients with acute myocardial infarction (AMI). Our preliminary study revealed that the CatLet score better predicted clinical outcomes for AMI patients than the Synergy between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery score. However, the reproducibility of the CatLet score in both inter- and intra-observer remains to be evaluated. Methods:. A total of 30 consecutive AMI patients, admitted in September of 2015, were independently assessed by two experienced interventional cardiologists to evaluate the inter-observer reproducibility of the CatLet score. Another set of 49 consecutive AMI patients, admitted between September and October in 2014, were assessed by one of the two interventional cardiologists on two occasions 3 months apart to evaluate the intra-observer reproducibility of the CatLet score. The weighted kappa was used to express the degree of agreement. Results:. The weighted kappa values (95% confidence interval) for the intra- and inter-observer reproducibility of the CatLet Score were 0.82 (0.59–1.00, Z = 7.23, P 22). Regarding the adverse characteristics pertinent to lesions and dominance parameters, the kappa values for the inter-observer variability were 0.80 (0.56–1.00, Z = 6.47, P

An active miniature inverted-repeat transposable element (MITE), MCLas-A, was previously identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). To explore the recent transposition status of MCLas-A, 389 ‘Ca. L. asiaticus’ strains collected from nine regions in China were amplified using a specific primer set and three representative ‘Ca. L. asiaticus’ strains were analyzed by next-generation sequencing (NGS) approach. PCR and genomic analysis showed that the entire MCLas-A was only present in 1.80% (7/389) and the jumping-out type of the MITE was predominant (81.23%) in samples tested, suggesting high frequency transposition occurred in ‘Ca. L. asiaticus’ strains recently collected from China. Biological roles of transposition of the active MITE remain to be determined.

To evaluate short-term effects of a four-week gait retraining program using visual feedback on trunk flexion angle, patellofemoral joint (PFJ) stress, lower extremity biomechanics and motor skill automaticity.Longitudinal interventional study.University research laboratory.Twelve asymptomatic recreational runners (seven male and five female).Trunk kinematics as well as lower extremity kinematics and kinetics were assessed prior to training at week 1 (baseline) and week 2, 3, 4 and 8 (retention). PFJ stress was computed using a sagittal plane model. A dual-task procedure was performed to examine automaticity.At week 8, runners demonstrated 10.1° increase in trunk flexion angle (p .001) and 17.8% reduction in peak PFJ stress (p .001) compared to baseline. This is associated with a 16.8% decrease in knee extensor moment and less than 2.5° change in knee flexion angle. Participants also showed 33.3% increase in peak hip extensor moment and small reduction in peak ankle plantar flexor moment. Lastly, runners demonstrated automaticity of the modified skill with a dual-task cost of less than 3%.The gait retraining program is effective to elicit short term changes in trunk position, PFJ stress, and automaticity of the new motor skill.

A Gram-stain-negative, rod-shaped, facultative anaerobic bacterium, designated strain 3539T, was isolated from coastal sediment of Weihai, PR China. Optimal growth occurred at 28 °C, pH 7.5–8.0 and in the presence of 3.0 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 3539T formed a robust clade with members of the genus Marinicella and was closely related to Marinicella litoralis JCM 16154T, Marinicella sediminis F2T and Marinicella pacifica sw153T with 97.7, 96.2 and 95.4 % sequence similarity, respectively. The average amino acid identity, percentage of conserved proteins, average nucleotide identity and digital DNA–DNA hybridization values between strain 3539T and M. litoralis JCM 16154T were 64.9, 68.3, 72.8 and 18.9 %, respectively. The genomic DNA G+C content of strain 3539T was 42.0 mol%. The dominant respiratory quinone was ubiquinone-8, and the major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c). The polar lipids of strain 3539T consisted of phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminophospholipid, one unidentified lipid and three unidentified phospholipids. Based on the combination of phylogenetic, phenotypic and chemotaxonomic data, strain 3539T is considered to represent a novel species within the genus Marinicella in he family Alcanivoracaceae , for which the name Marinicella rhabdoformis sp. nov. is proposed. The type strain of the new species is 3539T (=KCTC 72414T=MCCC 1H00388T).

Background. The aim of this study was to explore the relationship between coagulation/fibrinolysis function and serum lipid levels in people with advanced knee osteoarthritis (KOA).Methods. From January 1st 2013 to January 1st 2018, a total of 206 patients underwent total or unicompartmental knee arthroplasty in Changzhou No.1 People’s Hospital were enrolled consecutively in this study. Baseline characteristics and preoperative laboratory indicators of patients were collected. The association between coagulation/fibrinolysis function and serum lipid levels was analyzed using Spearman correlation analysis and multiple linear regression.Results. Spearman correlation analysis revealed that coagulation/fibrinolysis function was associated with serum lipid levels, especially prothrombin time was moderately negatively correlated with Apolipoprotein-A1 (r=-0.4585, PConclusions. The results of this study showed that coagulation/fibrinolysis function was correlated with serum lipid levels in patients with advanced KOA, although the correlation may be small. It may be hypothesized that strategies for lipid control may help improve coagulation/fibrinolytic dysfunction in patients with KOA.Trial registration. This clinical study was retrospectively registered in a publicly available registry because this study was a retrospective study began in 2018. This study was approved by the Ethics Committee of the Third Affiliated Hospital of Soochow University in Jiangsu Province of China (No.CZYY2021187).

Introduction: Lifestyle interventions incorporating exercise and nutrition can help prevent and manage cardiometabolic disease. However, not all interventions readily reach individuals who are most in need. A mobile health (mHealth) exercise program could be a cost-effective approach to broadening accessibility and providing support for physical activity behavior change. We tested the feasibility and acceptability of a mHealth exercise intervention in this 8-week pilot study. Hypothesis: A remotely delivered mHealth lifestyle program involving a wearable sensor, mobile application and social support is feasible and acceptable in sedentary adults. Methods: A total of 84 sedentary (< 30 min/week of exercise) adults (females 31.5 ± 11.3 yr, males 34.1 ± 28.9 yr, mean ± SD) were randomized (1:1:1) to 8 weeks of increasing physical activity behavioral support: Level 1 (wellness education, n=26), Level 2 (wellness education + pre-recorded exercise videos, n=30), or Level 3 (wellness education + livestream group exercise, n=28). Participants used a chest strap sensor (Myzone MZ-3) during exercise and a mobile application for interaction. A weekly email was used to deliver wellness education and encourage physical activity. Level 2 exercise videos and Level 3 livestream (Zoom) group classes were 35 min long, instructor-led, delivered 3x/wk and designed to meet national guidelines for weekly physical activity. Participants were surveyed at baseline and 8 weeks. Feasibility was defined through accrual and attrition; acceptability through sensor use (3x/wk encouraged) and survey data. Results: Between August 2020 and August 2021, 204 adults were screened for eligibility. Out of 135 eligible participants, 84 (62%) enrolled in the study. Preliminary analysis indicated 65% retention at 8-week follow-up. Participants were primarily Hispanic/Latinx (47%), 71% had 2-year college or more education, 45% reported income below federal poverty thresholds, and 36% reported qualifying for public assistance. Program acceptability varied by group with weekly median sensor use of 1 day/wk (Level 1), 2 days/wk (Level 2) and 3 days/wk (Level 3). Good-to-high program enjoyment was reported by 90% of Level 3 and 83% of Level 2 participants. Conclusions: This pilot study demonstrates that a livestream group-based or video-based exercise program is feasible and acceptable in sedentary adults. A randomized controlled trial investigating health-related outcomes using this mHealth program is warranted.